sp|Q9FNQ1|DEXHE_ARATH ----> DExH-box ATP-dependent RNA helicase DExH14

Intron 1 GTAGATTCGT ... GTGCATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	8	15	1
A2	20	4	2	0
A3	36.84	12	11	3
A4	53.49	10	15	8
A5	57.89	4	8	3
A6	34.38	21	16	6
A7	31.43	12	2	9
A8	28	9	5	2
A9	35.71	9	5	5
A10	26.09	17	7	5

>TRINITY_DN43227_c0_g2_i10 from sample A7 / Example spliced isoform

CTTAATTTAATTATATTTATGCTTATTGCTGGTGCGCCATTGAGGAGACCAATTGCTCCTCAGCAGGAAGAATATTTCATAGTGGTACCCAGAAGATAATACTTGGTACCCAACAGTATCTTCAAGCAAGTGTGATTAAAGAAAGGTGTGCCTGTGAATACTGTCAGGCGGTTCAACCAAAAGCTTTATGGGAAGCAAGT
GCAAGGATTGGGGAATGTACAAAGAGAATTCTCTTGCTATAGATTGATCAATTTCAATTTTTGTGCGATTTGAAGAATCAGAGAAGCAGTCTTTATGCCTCTAAACCAGTTCCCTCGTTTGACTATGAAGCTGAGAGCGTCCAGGCATCAATCAGAGTCTGTGTATGACATTGAGAAGGCCTATTTGGAACGCAAGCAGA
TCCTTCAGAATAAGCATTGCAGAAGGTATGAGTGGTATATGTTGCAGAAGGAAAAAGGAAACCATGGACATATGCCAACCTCTTACCATTGGATAGAATTGTTTATATAATGTGTATTTTTTTAGCACTCGGTCTCTTATGTTTCACACAGTTCTATTCCACCAAGTATTGTGGTCAAATAATTATAGTGCTTGCTGGAT
TCAGAATAACTTGCGTGAATTTATGGATATATATTCTCTTTGCCATGAAAAAAAAAAAAAAACTGGACTTCCTAAGAACTTGGTCTTAATGACTTAAGTTAGAAGGGGGATACAAAATTCTTGTATTTTGCTGGAA

>TRINITY_DN43227_c0_g2_i1 from sample A7 / Example unspliced isoform:

GGAATTATTTACAAAATTGAAAGAAAACTTCAATGGTGAGAAACTCAAGGCAGGCATTCCGCTTGCAAGACAAATCACTACCTATCTCCCAAGAAAGAGTAAGCGGGGGCCGTCTTCTCTAGTATGAAGGTACTCCACTAGGCGTCGGTTCACAGATTGCAAGACGTACTGATGAAAAACTCAGCTCTTAATTTTGAGTT
CTGCTTTCGAGTCCGTGGGAATCCCTGCAGGCAGGAAGTGTCCGCCAACACAGATGAACAAGATAAGGAAACTAAAACATGATATGATACACTGACAAGTAATGTTTTCAACTATTTTTTTGTAATGCAGATATTAATAATGACAAGTAGGGTGAATTGGGCTCTTTATTTGAAGGGATGGTTTTGATGACGCCCTCCCA
AAGTGATACCCTGGAAACCCCAGCACAACTCCAGCAAAATCCAGAGAATTGCCCTCCATCGGCATTAGTGTTGCTCCTCAGCAGGAAGAATATTTCATAGTGGTACCCAGAAGATAATACTTGGTACCCAACAGTATCTTCAAGCAAGTGTGATTAAAGAAAGGTGTGCCTGTGAATACTGTCAGGCGGTTCAACCAAAA
GCTTTATGGGAAGCAAGTGCAAGGATTGGGGAATGTACAAAGAGAATTCTCTTGCTATAGATTGATCAATTTCAATTTTTGTGCGATTTGAAGAATCAGAGAAGCAGTCTTTATGCCTCTAAACCAGTTCCCTCGTTTGACTATGAAGCTGAGAGCGTCCAGGCATCAATCAGAGTCTGTGTATGACATTGAGAAGGCCT
ATTTGGAACGCAAGCAGATCCTTCAGAATAAGCATTGCAGAAGgtagattcgttatcttcccactttatatttatattcaaatcactgttcttggttgaagaaataaatggatttattataaaaacacaatcacacagaagactacagtgagcctccagtgatgaatgccttggtttagtaaggcattgggatccttgtt
ttcttttgcgtgtgtgcatgcagGTATGAGTGGTATATGTTGCAGAAGGAAAAAGGAAACCATGGACATATGCCAACCTCTTACCATTGGATAGAATTGTTTATATAATGTGTATTTTTTTAGCACTCGGTCTCTTATGTTTCACACAGTTCTATTCCACCAAGTATTGTGGTCAAATAATTATAGTGCTTGCTGGATTC
AGAATAACTTGCGTGAATTTATGGATATATATTCTCTTTGCCATGAAAAAAAAAAAAAAACTGGACTTCCTAAGAACTTGGTCTTAATGACTTAAGTTAGAAGGGGGATACAAAATTCTTGTATTTTGCTGGAA

Intron 2 GCTCCTCAGC ... ATGTACAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	34.69	16	0	17
A2	40	9	3	9
A3	33.33	16	2	14
A4	31.15	21	2	17
A5	34.78	15	8	8
A6	16.36	46	6	12
A7	28.57	20	4	12
A8	18.52	22	5	5
A9	12.5	28	3	5
A10	15.25	25	6	3

>TRINITY_DN43227_c0_g2_i8 from sample A7 / Example spliced isoform

GGAATTATTTACAAAATTGAAAGAAAACTTCAATGGTGAGAAACTCAAGGCAGGCATTCCGCTTGCAAGACAAATCACTACCTATCTCCCAAGAAAGAGTAAGCGGGGGCCGTCTTCTCTAGTATGAAGGTACTCCACTAGGCGTCGGTTCACAGATTGCAAGACGTACTGATGAAAAACTCAGCTCTTAATTTTGAGTT
CTGCTTTCGAGTCCGTGGGAATCCCTGCAGGCAGGAAGTGTCCGCCAACACAGATGAACAAGATAAGGAAACTAAAACATGATATGATACACTGACAAGTAATGTTTTCAACTATTTTTTTGTAATGCAGATATTAATAATGACAAGTAGGGTGAATTGGGCTCTTTATTTGAAGGGATGGTTTTGATGACGCCCTCCCA
AAGTGATACCCTGGAAACCCCAGCACAACTCCAGCAAAATCCAGAGAATTGCCCTCCATCGGCATTAGTGTTAGAATTCTCTTGCTATAGATTGATCAATTTCAATTTTTGTGCGATTTGAAGAATCAGAGAAGCAGTCTTTATGCCTCTAAACCAGTTCCCTCGTTTGACTATGAAGCTGAGAGCGTCCAGGCATCAAT
CAGAGTCTGTGTATGACATTGAGAAGGCCTATTTGGAACGCAAGCAGATCCTTCAGAATAAGCATTGCAGAAGGTCTGCGACTTTGGTTGGTAATTCTGATCTTGCATAGAAGATTGTACCTGGATGGGAAACAGGTAATAGATAATTATTCTTGATGGCAAATGCTAAGTAGATTTGGCATCTTTGTTGTTTAGGTGTG
ATGCACCAGTGAGCTTATCACTTATTTGCAATTGCATATCTGCTTAATGGTAGACTGTTATAAGATCTTGACAAAGTGAATTCTTTTGTTTCTTTCAATGCTAGAGCTGATTTGGACATGGATAAATTTTTTTAAATTATGCACGGTTAAGGTGTCATGGAGGTCACGCATGACACCTTTAGCAAAACTGCATATTTCGG
TTTTTGCTTTATTGTTTTTTTTTTGTGGGGAAAGGGCAGTGCTTTTGTGGTGAAGAAAGGTGGCAACTTATTCATATCTCCAAATTTGTGAATGCTTGGCTTTTAAGACCTGATTATATGTTTAATGATTCATGGTTATTCTCTGAC

>TRINITY_DN43227_c0_g2_i1 from sample A7 / Example unspliced isoform:

GGAATTATTTACAAAATTGAAAGAAAACTTCAATGGTGAGAAACTCAAGGCAGGCATTCCGCTTGCAAGACAAATCACTACCTATCTCCCAAGAAAGAGTAAGCGGGGGCCGTCTTCTCTAGTATGAAGGTACTCCACTAGGCGTCGGTTCACAGATTGCAAGACGTACTGATGAAAAACTCAGCTCTTAATTTTGAGTT
CTGCTTTCGAGTCCGTGGGAATCCCTGCAGGCAGGAAGTGTCCGCCAACACAGATGAACAAGATAAGGAAACTAAAACATGATATGATACACTGACAAGTAATGTTTTCAACTATTTTTTTGTAATGCAGATATTAATAATGACAAGTAGGGTGAATTGGGCTCTTTATTTGAAGGGATGGTTTTGATGACGCCCTCCCA
AAGTGATACCCTGGAAACCCCAGCACAACTCCAGCAAAATCCAGAGAATTGCCCTCCATCGGCATTAGTGTTgctcctcagcaggaagaatatttcatagtggtacccagaagataatacttggtacccaacagtatcttcaagcaagtgtgattaaagaaaggtgtgcctgtgaatactgtcaggcggttcaaccaaaa
gctttatgggaagcaagtgcaaggattggggaatgtacaaagAGAATTCTCTTGCTATAGATTGATCAATTTCAATTTTTGTGCGATTTGAAGAATCAGAGAAGCAGTCTTTATGCCTCTAAACCAGTTCCCTCGTTTGACTATGAAGCTGAGAGCGTCCAGGCATCAATCAGAGTCTGTGTATGACATTGAGAAGGCCT
ATTTGGAACGCAAGCAGATCCTTCAGAATAAGCATTGCAGAAGGTAGATTCGTTATCTTCCCACTTTATATTTATATTCAAATCACTGTTCTTGGTTGAAGAAATAAATGGATTTATTATAAAAACACAATCACACAGAAGACTACAGTGAGCCTCCAGTGATGAATGCCTTGGTTTAGTAAGGCATTGGGATCCTTGTT
TTCTTTTGCGTGTGTGCATGCAGGTATGAGTGGTATATGTTGCAGAAGGAAAAAGGAAACCATGGACATATGCCAACCTCTTACCATTGGATAGAATTGTTTATATAATGTGTATTTTTTTAGCACTCGGTCTCTTATGTTTCACACAGTTCTATTCCACCAAGTATTGTGGTCAAATAATTATAGTGCTTGCTGGATTC
AGAATAACTTGCGTGAATTTATGGATATATATTCTCTTTGCCATGAAAAAAAAAAAAAAACTGGACTTCCTAAGAACTTGGTCTTAATGACTTAAGTTAGAAGGGGGATACAAAATTCTTGTATTTTGCTGGAA

Intron 3 GCTCCTCAGC ... CGATTTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.54	11	0	52
A2	90	3	3	27
A3	71.05	22	2	54
A4	82.35	6	2	28
A5	72.22	10	8	26
A6	56.41	17	6	22
A7	73.91	6	4	17
A8	50	11	5	11
A9	60	8	3	12
A10	55.56	4	6	5

>TRINITY_DN43227_c0_g2_i9 from sample A7 / Example spliced isoform

GGAATTATTTACAAAATTGAAAGAAAACTTCAATGGTGAGAAACTCAAGGCAGGCATTCCGCTTGCAAGACAAATCACTACCTATCTCCCAAGAAAGAGTAAGCGGGGGCCGTCTTCTCTAGTATGAAGGTACTCCACTAGGCGTCGGTTCACAGATTGCAAGACGTACTGATGAAAAACTCAGCTCTTAATTTTGAGTT
CTGCTTTCGAGTCCGTGGGAATCCCTGCAGGCAGGAAGTGTCCGCCAACACAGATGAACAAGATAAGGAAACTAAAACATGATATGATACACTGACAAGTAATGTTTTCAACTATTTTTTTGTAATGCAGATATTAATAATGACAAGTAGGGTGAATTGGGCTCTTTATTTGAAGGGATGGTTTTGATGACGCCCTCCCA
AAGTGATACCCTGGAAACCCCAGCACAACTCCAGCAAAATCCAGAGAATTGCCCTCCATCGGCATTAGTGTTAATCAGAGAAGCAGTCTTTATGCCTCTAAACCAGTTCCCTCGTTTGACTATGAAGCTGAGAGCGTCCAGGCATCAATCAGAGTCTGTGTATGACATTGAGAAGGCCTATTTGGAACGCAAGCAGATCC
TTCAGAATAAGC

>TRINITY_DN43227_c0_g2_i1 from sample A7 / Example unspliced isoform:

GGAATTATTTACAAAATTGAAAGAAAACTTCAATGGTGAGAAACTCAAGGCAGGCATTCCGCTTGCAAGACAAATCACTACCTATCTCCCAAGAAAGAGTAAGCGGGGGCCGTCTTCTCTAGTATGAAGGTACTCCACTAGGCGTCGGTTCACAGATTGCAAGACGTACTGATGAAAAACTCAGCTCTTAATTTTGAGTT
CTGCTTTCGAGTCCGTGGGAATCCCTGCAGGCAGGAAGTGTCCGCCAACACAGATGAACAAGATAAGGAAACTAAAACATGATATGATACACTGACAAGTAATGTTTTCAACTATTTTTTTGTAATGCAGATATTAATAATGACAAGTAGGGTGAATTGGGCTCTTTATTTGAAGGGATGGTTTTGATGACGCCCTCCCA
AAGTGATACCCTGGAAACCCCAGCACAACTCCAGCAAAATCCAGAGAATTGCCCTCCATCGGCATTAGTGTTgctcctcagcaggaagaatatttcatagtggtacccagaagataatacttggtacccaacagtatcttcaagcaagtgtgattaaagaaaggtgtgcctgtgaatactgtcaggcggttcaaccaaaa
gctttatgggaagcaagtgcaaggattggggaatgtacaaagagaattctcttgctatagattgatcaatttcaatttttgtgcgatttgaagAATCAGAGAAGCAGTCTTTATGCCTCTAAACCAGTTCCCTCGTTTGACTATGAAGCTGAGAGCGTCCAGGCATCAATCAGAGTCTGTGTATGACATTGAGAAGGCCT
ATTTGGAACGCAAGCAGATCCTTCAGAATAAGCATTGCAGAAGGTAGATTCGTTATCTTCCCACTTTATATTTATATTCAAATCACTGTTCTTGGTTGAAGAAATAAATGGATTTATTATAAAAACACAATCACACAGAAGACTACAGTGAGCCTCCAGTGATGAATGCCTTGGTTTAGTAAGGCATTGGGATCCTTGTT
TTCTTTTGCGTGTGTGCATGCAGGTATGAGTGGTATATGTTGCAGAAGGAAAAAGGAAACCATGGACATATGCCAACCTCTTACCATTGGATAGAATTGTTTATATAATGTGTATTTTTTTAGCACTCGGTCTCTTATGTTTCACACAGTTCTATTCCACCAAGTATTGTGGTCAAATAATTATAGTGCTTGCTGGATTC
AGAATAACTTGCGTGAATTTATGGATATATATTCTCTTTGCCATGAAAAAAAAAAAAAAACTGGACTTCCTAAGAACTTGGTCTTAATGACTTAAGTTAGAAGGGGGATACAAAATTCTTGTATTTTGCTGGAA