sp|P68430|H32_ONOVI ----> Histone H3.2

Intron 1 GTGTGTTTTC ... GCCTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.44	4.3	21.7	5
A2	97.53	0.7	22	0
A3	85.19	5.3	23.3	0
A4	88.21	2	15.7	2
A5	100	0	8.7	0
A6	90.23	90	724.7	12
A7	89.83	39.7	316	7
A8	89.32	66	506.3	16
A9	89.63	59.3	465.7	18
A10	90.95	25	220.7	5

>TRINITY_DN44146_c1_g1_i1 from sample A9 / Example spliced isoform

GCCGGGCACTGTTGCTCTCCGTGAAATCCGAAAATACCAGAAGAGTACCGAGCTTCTGATAAGAAAGCTGCCCTTCCAAAGGCTCGTAAGGGAGATTGCCCAGGATTTCAAGACAGACTTGAGGTTCCAGTCGTCAGCAGTAGCTGCACTGCAAGAAGCAGCAGAGGCATACCTCGTTGGCCTCTTCGAAGATACCAACC
TGTGCGCCATTCATGCTAAGAGGGTTACAATAATGCCCAAAGATATCCAGCTCGCTCGCCGCATCCGTGGAGAGAGGGCATAAACGTGCATTCATAATGCTTTTGCCTATCATTGTCAATGGGTAGTTTATTCTGATAATGCTAAAACCATTAAAGGAATGCATTGATTCTAAATGCCGCTATAAGCTATCCACAGCAAG
TTTGTAGCGGTTATATCCAATAAGGTGAATTGTGTGCTCTCGGCTGCAGAAATTAAGTGCATTGGTTTATAAATTGTTTAAGCTATCTAATTATTAGACAAATCATAGAAAACCAAGTTAAAGAAATATTAGATTCTTTGATTTTTTTGTTTGGCTTGTGATTTTGTTTATTGGGTTTGTAGCTTGAAGAGGCCTTTATT
TAGTTTGTTCTTGGGAGTTTGTTGAGGATTGTTACAAGTCCAGGATCTATTTTTATTTTTATTTTTATTTTGAAGGAGAGGGAGAATTAGCAAAACACCTGGGATGCAATTTGCACCATGCACCATGGCTTTTTTCTAAATCATCCATTTGCATCTTTAGATTTTTGCTATGTTGAATGTTTGGGCTGTTCTAGGTGGCC
CCTCATAAGGGGATCCTCAAATGTGAGTACTTTTTCTTGGAAGGAACAGACTAAAGTTTAAAGCTTAGCTTGGTTAGGTTAGGGTCAGGTAATTTTTGCAGAATTTATGGCACTAAAGTTGAGTAATACTGTGATAGTTGTAAGAAAGAGTCTCTAAACTTTAAATGGAGGAAACTCCAATGGAGCGAGTGATATGATCC
ACATCATTAATGGCCGATGCATTGTAGAAGTTGAGATCTCTTTGAATTCCATGTGATACA

>TRINITY_DN44146_c1_g1_i2 from sample A9 / Example unspliced isoform:

GCCGGGCACTGTTGCTCTCCGTGAAATCCGAAAATACCAGAAGAGTACCGAGCTTCTGATAAGAAAGCTGCCCTTCCAAAGGCTCGTAAGGGAGATTGCCCAGGATTTCAAGACAGACTTGAGGTTCCAGTCGTCAGCAGTAGCTGCACTGCAAGAAGCAGCAGAGGCATACCTCGTTGGCCTCTTCGAAGATACCAACC
TGTGCGCCATTCATGCTAAGAGGGTTACAATAATGCCCAAAGATATCCAGCTCGCTCGCCGCATCCGTGGAGAGAGGGCATAAACGTGCATTCATAATGCTTTTGCCTATCATTGTCAATGgtgtgttttcacaccaccatttggttctttaaatttgttctccctattattttcctttaatctgaaacagatttgtgcg
aaatttctcaatttcatgcatttagtagcagccgcataataatgcatctacacacggagtttcgatcctgggatctgtgtatgtttttaatattttgctgacttgtatatttgaattcagggtaatcttgtgtgagtgttgctattatggttttattcttgtgctgatagtgtgtcttatttcagcgtattcttgccttt
gcagGGTAGTTTATTCTGATAATGCTAAAACCATTAAAGGAATGCATTGATTCTAAATGCCGCTATAAGCTATCCACAGCAAGTTTGTAGCGGTTATATCCAATAAGGTGAATTGTGTGCTCTCGGCTGCAGAAATTAAGTGCATTGGTTTATAAATTGTTTAAGCTATCTAATTATTAGACAAATCATAGAAAACCAAG
TTAAAGAAATATTAGATTCTTTGATTTTTTTGTTTGGCTTGTGATTTTGTTTATTGGGTTTGTAGCTTGAAGAGGCCTTTATTTAGTTTGTTCTTGGGAGTTTGTTGAGGATTGTTACAAGTCCAGGATCTATTTTTATTTTTATTTTTATTTTGAAGGAGAGGGAGAATTAGCAAAACACCT

Intron 2 GTGTGTTTTC ... TCATTAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76.87	34	113	26
A2	70.8	33	80	8
A3	87.22	17	116	8
A4	80.91	21	89	7
A5	81.82	14	63	4
A6	81.64	330	1467	70
A7	83.47	215	1086	72
A8	84.16	337	1790	70
A9	80.82	346	1458	71
A10	82.08	150	687	67

>TRINITY_DN106272_c1_g1_i10 from sample A4 / Example spliced isoform

TATTAAGGGTTTGTTCAATTCGGAGTTGCAGATCAGAGGAGAAAGAAATCGCAGGGAGACAGAAGAAAAGAGAAGAGGAGATGGCACGAACAAAGCAGACTGCGCGTAAATCAACAGGGGGTAAGGCACCACGGAAGCAGCTGGCAACCAAGGCTGCAAGGAAGTCTGCACCTGCCACCGGTGGAGTGAAGAAACCTCAC
AGATTCAGGCCCGGCACAGTCGCTCTCCGTGAAATTCGAAAATACCAGAAGAGCACCGAGCTTCTGATAAGAAAGCTTCCCTTCCAGAGGCTTGTGAGGGAGATTGCTCAAGATTTCAAGACAGACTTGAGATTCCAGTCGTCAGCGGTAGCGGCATTGCAAGAAGCGGCTGAGGCATATCTTGTTGGGCTGTTTGAGGA
CACAAACCTGTGCGCCATCCACGCCAAGAGGGTTACAATAATGCCCAAGGATATCCAGCTTGCTCGCCGCATCCGTGGAGAGAGGGCATAAACTTCATAACTTATAATTATGCAATGCCAGTGCATTTACCTACAGTGTTAATGGTACGAATCTATTAAATGATAGTTGTGAAGGAGAAAAAATAATATTTAATTCTTAG
ATTCTTCAGGTGCAATGATGGGAACCTATTAAATGGTGCTGAAAATAAAAAGACCGAACCC

>TRINITY_DN106272_c1_g1_i2 from sample A4 / Example unspliced isoform:

TATTAAGGGTTTGTTCAATTCGGAGTTGCAGATCAGAGGAGAAAGAAATCGCAGGGAGACAGAAGAAAAGAGAAGAGGAGATGGCACGAACAAAGCAGACTGCGCGTAAATCAACAGGGGGTAAGGCACCACGGAAGCAGCTGGCAACCAAGGCTGCAAGGAAGTCTGCACCTGCCACCGGTGGAGTGAAGAAACCTCAC
AGATTCAGGCCCGGCACAGTCGCTCTCCGTGAAATTCGAAAATACCAGAAGAGCACCGAGCTTCTGATAAGAAAGCTTCCCTTCCAGAGGCTTGTGAGGGAGATTGCTCAAGATTTCAAGACAGACTTGAGATTCCAGTCGTCAGCGGTAGCGGCATTGCAAGAAGCGGCTGAGGCATATCTTGTTGGGCTGTTTGAGGA
CACAAACCTGTGCGCCATCCACGCCAAGAGGGTTACAATAATGCCCAAGGATATCCAGCTTGCTCGCCGCATCCGTGGAGAGAGGGCATAAACTTCATAACTTATAATTATGCAATGCCAGTGCATTTACCTACAGTGTTAATGgtgtgttttcacaccaccacctgcttctttaaatttgttttccctcgacttctctc
ttttatctttcttttgcattcttgaattatgtacaacaaaataagtttgcccgatcttaattgattttttttttttttttcttatgtttatgcatcattagcagGTACGAATCTATTAAATGATAGTTGTGAAGGAGAAAAAATAATATTTAATTCTTAGATTCTTCAGGTGCAATGATGGGAACCTATTAAATGGTGCT
GAAAATAAAAAGACCGAACCC