sp|Q39033|PLCD2_ARATH ----> Phosphoinositide phospholipase C 2

Intron 1 GTAACATAGC ... AAATCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	11.32	525	67	34.8
A2	11.51	415	54	29.6
A3	10.41	508	59	26
A4	10.88	303	37	18
A5	5.09	205	11	10.4
A6	7.24	1141	89	67.2
A7	8.71	2108	201	108.4
A8	8.92	1961	192	97.2
A9	9.58	1868	198	110
A10	8.74	1587	152	72

>TRINITY_DN41094_c0_g1_i2 from sample A7 / Example spliced isoform

GAAGGATTAAAAGGATAAATTTTGCCTTGATGCGTCCAAATGGTTCTTCCGCCTGCACCAGATTTTGCAGTCGGTGACCAGTAGGTTTAAGCAAATATGGAATTAATTATCATTTATTTATTATACTGTATAGTACGTGTAAAATCCGCGTGCCCTTGACACGAAAACCCAACAAATTCGGACCATGACTCGGCTGTTCG
AAGCATTTGAATAGGCGTTGTGCCCACGCAGTCCAGCCTTCTGCTATATACATTGCATCACAGTGTTCCTGTGACCGCGGTAGGCAACGATAAGGTGCAGAATCGAACCCGCGACGAATGGGGAGCTACAGAGTATGCATCTGCTTCACCCGCAAGTTCAAATCTGGCGCCCTGAGCCCGCCCAAGGATGTCAAGGATCT
GTTCGACAAGTACTCGGAGAGCGGCGGGCAGATGAGCGTCAGGAATCTCCAGCGATTTCTCGTCGAAGTTCAGGGGCAGAACGACGCCACCGAGAAGGACGCCGAGAATGTCATCGAAGTGCTGCGGCAGCGCAGGCATGTGAGCGTCTTCCACCGTCACGGTATCAATCTCGACGGATTCTTCCATTTTCTCTTCAGCG
ACGATCTTAACCCGGTCATCGATTCTAAGGTCCACCATGATATGACAGCACCATTGTCTCACTACTTCATATATACAGGTCATAATTCATATCTAACTGGAAATCAGCTGAGCAGTGACTGTAGCGATGCACCTATCATCAAAGCTCTTAAGAGAGGTGTACGGGTCATAGAGTTGGATATTTGGCCAAATTCAGCAAAA
GATGATGTGCATGTCCTTCATGGAAGGACCTTGACCACTCCTGTAGAGTTGATTAAATGTCTTAGGTCAATAAAAGAGCACGCCTTTGTTGCATCACAGTATCCTGTGATAATAACATTGGAGGATCACCTTACTCGAGATCTCCAAGCCAAAGCAGCTGAGATGATCACCAAAACCTTTGGCGATTTGCTCTACTACCC
CAAATCAGAAGTCTTAGAAGAGTTCCCTTCACCAGAATTCTTGAAGGGTCGCATAATCATCTCAACTAAACCTCCAAAGGAGTACTTAGAAGTTAAAACCACTGAAGTCCAGGGAAGTGGGGACAAGGAGAAGCTCAAGACACCTGATGAAGAGCCGTGGGGTGATGACATCCCGGATTATGGCTCTGATGTGCTAGGCG
AACGGGAGGTGATAATATATACTCAATTCAAGTCTTTTCGTGGGCACTCTTATCTACTTGTCCATACTTTCCTACGTTGTTGATATGGATCCCTTTATCCAGAAAAAGTCAATGCAGCGTGTGCAGTTGCATTCTAGCTGGATTTTAAATTTCCGCCTCCCTCTTGCTGTCGATTCTAAGATCTTAAAGTCTGTACTGGC
AGAGTGCAAATGTTGCTGGACACATTGAAGAAGATTCTGATGATGATGAAAGCATTTCACAAGCGAATCAAGATAAAGATGTACCACCTGAGTATAAGCGCCTTATAACCATTAGAGCAGGGAAGCCAAAAGGAGTGTCTTTGAAAGATGCTATAAGAGTTGACAGTAAGCAGGTCAAGCGGGTTAGTTTAAGTGAACCA
CAACTTCAAAAGGTCTCTAGGTCTCATCCAACCCATATTGTTAGATTTACCCACAATAACTTCTTGAGAATATATCCAAAGGGTACTCGTATTGATTCATCAAACTATAACCCTTTGGTTGCTTGGACTCATGGAGCTCAGATGGTGGCCTTAAATATGCAGGGATATGGAAGGCAGCTTTGGTTAGTGCAGGGTTTCTT
TAGAGCCAATGGGGGCTGCGGTTATGTCAAAAAGCCTGACTTTCTTCTTTCAAACGGATCTAAAGATGATGACCAACATATTTTTAATCCAAAGACACCGGGAGCAGTCAAGACTACACTGAAGGTCAATATATATATGGGAGATGGATGGAATCAGGATTTCAAGAAAACACACTTTGACACCTATTCTCCGCCGGACT
TTTACAGCAGGGTTGGGATTGCAGGTGTGCCTGCAGATACTATCATGAAAAAGACAAGGGCGATAGAAGATGACTGGGTTCCGTGTTGGAATGAGGAGTTTGTTTTTCCATTGACTGTCCCTGAATTAGCTTTGCTTCGTATTGAAGTGCACGAGTATGATATGTCTGAGAAAGATGACTTTGGTGGCCAGACATGCTTA
CCTGTGGCAGAGTTGAAGTCAGGGATTCGCTCAATTCCGCTATATGATAAGAAAGGAGAAAAATACAAGTCTGTAAGACTTTTGGTGGGAATCTCTAAGACTTGATTTCTACTAGCAATCATTCCTGCCGGGATAAGCGTCTTGTAATATATATCTATGAATCCAATTTGGGATTCTGGTTTAGTTTGGATTTTGGCAGG
GAAAGTCTGTCTTGTCTATTTCTACAGAGGATATTTGGTAGCCAAAATTGAGTCTGAAACACTCACTTTCTGATGTGATTACTGATGGTGGGAATCTCTAAGACTTATGGTGCGAATTGATTTCTACTAGCAATAATTCCTGCAGGGATAAAAGACTTGTAATATATATCTGTGAATCAAGTTTTGGGATTCTGGTTTAG
TTTGAATTTTGGCATGGGAGATCTAATTATCTTTGTCTACCTTTATTAATACGTTTTTGTAATAATTCTATTTTGTAACGTAGGATACAAATGTCTTTCGGAGATATTTTAGAATTCCTACAAAAAGATGTTTGGTAGCCAAAATTGAGTCTGCAGCATTCACTTTATGACGTGACTATTTAACCATCTTTCTCAGGGAG
TATATATTACAATGGATGTTCATGAAATAGCAATGAACAGTACATTTGGATTGAATTAATTTTCAGTAATTACTCGAGACTCGTACAATTCTAAGGATATCAGTACAACAAACAAA

>TRINITY_DN41094_c0_g1_i4 from sample A7 / Example unspliced isoform:

GAAGGATTAAAAGGATAAATTTTGCCTTGATGCGTCCAAATGGTTCTTCCGCCTGCACCAGATTTTGCAGTCGGTGACCAGTAGGTTTAAGCAAATATGGAATTAATTATCATTTATTTATTATACTGTATAGTACGTGTAAAATCCGCGTGCCCTTGACACGAAAACCCAACAAATTCGGACCATGACTCGGCTGTTCG
AAGCATTTGAATAGGCGTTGTGCCCACGCAGTCCAGCCTTCTGCTATATACATTGCATCACAGTGTTCCTGTGACCGCGGTAGGCAACGATAAGGTGCAGAATCGAACCCGCGACGAATGGGGAGCTACAGAGTATGCATCTGCTTCACCCGCAAGTTCAAATCTGGCGCCCTGAGCCCGCCCAAGGATGTCAAGGATCT
GTTCGACAAGTACTCGGAGAGCGGCGGGCAGATGAGCGTCAGGAATCTCCAGCGATTTCTCGTCGAAGTTCAGGGGCAGAACGACGCCACCGAGAAGGACGCCGAGAATGTCATCGAAGTGCTGCGGCAGCGCAGGCATGTGAGCGTCTTCCACCGTCACGGTATCAATCTCGACGGATTCTTCCATTTTCTCTTCAGCG
ACGATCTTAACCCGGTCATCGATTCTAAGGTCCACCATGATATGACAGCACCATTGTCTCACTACTTCATATATACAGGTCATAATTCATATCTAACTGGAAATCAGCTGAGCAGTGACTGTAGCGATGCACCTATCATCAAAGCTCTTAAGAGAGGTGTACGGGTCATAGAGTTGGATATTTGGCCAAATTCAGCAAAA
GATGATGTGCATGTCCTTCATGGAAGGACCTTGACCACTCCTGTAGAGTTGATTAAATGTCTTAGGTCAATAAAAGAGCACGCCTTTGTTGCATCACAGTATCCTGTGATAATAACATTGGAGGATCACCTTACTCGAGATCTCCAAGCCAAAGCAGCTGAGATGATCACCAAAACCTTTGGCGATTTGCTCTACTACCC
CAAATCAGAAGTCTTAGAAGAGTTCCCTTCACCAGAATTCTTGAAGGGTCGCATAATCATCTCAACTAAACCTCCAAAGGAGTACTTAGAAGTTAAAACCACTGAAGTCCAGGGAAGTGGGGACAAGGAGAAGCTCAAGACACCTGATGAAGAGCCGTGGGGTGATGACATCCCGGATTATGGCTCTGATGTGCTAGGCG
AACGGGAGAGTGCAAATGTTGCTGGACACATTGAAGAAGATTCTGATGATGATGAAAGCATTTCACAAGCGAATCAAGATAAAGATGTACCACCTGAGTATAAGCGCCTTATAACCATTAGAGCAGGGAAGCCAAAAGGAGTGTCTTTGAAAGATGCTATAAGAGTTGACAGTAAGCAGGTCAAGCGGGTTAGTTTAAGT
GAACCACAACTTCAAAAGGTCTCTAGGTCTCATCCAACCCATATTGTTAGATTTACCCACAATAACTTCTTGAGAATATATCCAAAGGGTACTCGTATTGATTCATCAAACTATAACCCTTTGGTTGCTTGGACTCATGGAGCTCAGATGGTGGCCTTAAATATGCAGGGATATGGAAGGCAGCTTTGGTTAGTGCAGGG
TTTCTTTAGAGCCAATGGGGGCTGCGGTTATGTCAAAAAGCCTGACTTTCTTCTTTCAAACGGATCTAAAGATGATGACCAACATATTTTTAATCCAAAGACACCGGGAGCAGTCAAGACTACACTGAAGGTCAATATATATATGGGAGATGGATGGAATCAGGATTTCAAGAAAACACACTTTGACACCTATTCTCCGC
CGGACTTTTACAGCAGGgtaacatagcaacttcttattaagaaatcttaatggtgtagcatattgagctcttaaaaaaagtttccaatgtggacattgcctgacaaacttaaatctgcagGTTGGGATTGCAGGTGTGCCTG

Intron 2 GTAACCCTGG ... TTCATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.37	459	140	20.5
A2	27.59	349.2	133	17
A3	25.71	390	135	12
A4	20.75	271.3	71	12.5
A5	28.55	192.7	77	7.5
A6	25.02	650.5	217	31
A7	22.33	1231.2	354	53.5
A8	24.87	1045.3	346	48
A9	24.4	1143.2	369	45.5
A10	22.24	1042.2	298	50

>TRINITY_DN106726_c0_g1_i3 from sample A3 / Example spliced isoform

CACAGTATCCTGTGATAATAACATTGGAGGATCACCTTACTCGAGATCTCCAAGCCAAAGCAGCTGAGATGATCACCAAAACCTTTGGCGATTTGCTCTACTACCCCAAATCAGAAGTCTTAGAAGAGTTCCCTTCACCAGAATTCTTGAAGGGTCGCATAATCATCTCAACTAAACCTCCAAAGGAGTACTTAGAAGTT
AAAACCACTGAAGTCCAGGGAAGTGGGGACAAGGAGAAGCTCAAGACACCTGATGAAGAGCCGTGGGGTGATGACATCCCGGATTATGGCTCTGATGTGCTAGGCGAACGGGAGAGTGCAAATGTTGCTGGACACATTGAAGAAGATTCTGATGATGATGAAAGCATTTCACAAGCGAATCAAGATAAAGATGTACCACC
TGAGTATAAGCGCCTTATAACCATTAGAGCAGGGAAGCCAAAAGGAGTGTCTTTGAAAGATGCTATAAGAGTTGACAGTAAGCAGGTCAAGCGGGTTAGTTTAAGTGAACCACAACTTCAAAAGGTCTCTAGGTCTCATCCAACCCATATTGTTAGATTTACCCACAATAACTTCTTGAGAATATATCCAAAGGGTACTC
GTATTGATTCATCAAACTATAACCCTTTGGTTGCTTGGACTCATGGAGCTCAGATGGTGGCCTTAAATATGCAGGGATATGGAAGGCAGCTTTGGTTAGTGCAGGGTTTCTTTAGAGCCAATGGGGGCTGCGGTTATGTCAAAAAGCCTGACTTTCTTCTTTCAAACGGATCTAAAGATGATGACCAACATATTTTTAAT
CCAAAGACACCGGGAGCAGTCAAGACTACACTGAAGGTCAATATATATATGGGAGATGGATGGAATCAGGATTTCAAGAAAACACACTTTGACACCTATTCTCCGCCGGACTTTTACAGCAGGGTTGGGATTGCAGGTGTGCCTGCAGATACTATCATGAAAAAGACAAGGGCGATAGAAGATGACTGGGTTCCGTGTTG
GAATGAGGAGTTTGTTTTTCCATTGACTGTCCCTGAATTAGCTTTGCTTCGTATTGAAGTGCACGAGTATGATATGTCTGAGAAAGATGACTTTGGTGGCCAGACATGCTTACCTGTGGCAGAGTTGAAGTCAGGGATTCGCTCAATTCCGCTATATGATAAGAAAGGAGAAAAATACAAGTCTGTAAGACTTTTGGTGG
GAATCTCTAAGACTTGATTTCTACTAGCAATCATTCCTGCCGGGATAAGCGTCTTGTAATATATATCTATGAATCCAATTTGGGATTCTGGTTTAGTTTGGATTTTGGCAGGGAAAGTCTGTCTTGTCTATTTCTACAGAGGATATTTGGTAGCCAAAATTGAGTCTGAAACACTCACTTTCTGATGTGATTACTGATGG
TGGGAATCTCTAAGACTTATGGTGCGAATTGATTTCTACTAGCAATAATTCCTGCAGGGATAAAAGACTTGTAATATATATCTGTGAATCAAGTTTTGGGATTCTGGTTTAGTTTGAATTTTGGCATGGGAGATCTAATTATCTTTGTCTACCTTTATTAATACGTTTTTGTAATAATTCTATTTTGTAACGTAGGATAC
AAATGTCTTTCGGAGATATTTTAGAATTCCTACAAAAAGATGTTTGGTAGCCAAAATTGAGTCTGCAGCATTCACTTTATGACGTGACTATTTAACCATCTTTCTCAGGGAGTATATATTACAATGGATGTTCA

>TRINITY_DN106726_c0_g1_i4 from sample A3 / Example unspliced isoform:

ATTGAAGAAGATTCTGATGATGATGAAAGCATTTCACAAGCGAATCAAGATAAAGATGTACCACCTGAGTATAAGCGCCTTATAACCATTAGAGCAGGGAAGCCAAAAGGAGTGTCTTTGAAAGATGCTATAAGAGTTGACAGTAAGCAGGTCAAGCGGGTTAGTTTAAGTGAACCACAACTTCAAAAGGTCTCTAGGTC
TCATCCAACCCATATTGTTAGgtaaccctggaacttccttttatttctagattctgaaattatatgcaattcattattgtttgtttgattttcaattagttgcatatttcttaatatttcattgcagATTTACCCACAATAACTTCTTGAGAATATATCCAAAGGGTACTCGTATTGATTCATCAAACTATAACCCTTTG
GTTGCTTGGACTCATGGAGCTCAGATGGTGGCCTTAAATATGCAGG

Intron 3 GTGATAATAT ... GTACTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.85	443	47.4	171
A2	28.86	387	33.6	157
A3	25.16	470	51.5	158
A4	32.56	290	30.5	140
A5	21.35	221	12.4	60
A6	27.14	553	23.6	206
A7	27.55	1273	93.1	484
A8	29.2	817	51	337
A9	26.81	1223.3	87.5	448
A10	26.21	1053	74.6	374

>TRINITY_DN115892_c1_g1_i5 from sample A1 / Example spliced isoform

AGAAGGTAAGCAAACATTGAGTTGATAGAAAAAAATGATTTGCAACAATTCACTTACCGTGTTCCTCTTTATTGCAGGTCCACCATGATATGACAGCACCATTGTCTCACTACTTCATATATACAGGTCATAATTCATATCTAACTGGAAATCAGCTGAGCAGTGACTGTAGCGATGCACCTATCATCAAAGCTCTTAAG
AGAGGTGTACGGGTCATAGAGTTGGATATTTGGCCAAATTCAGCAAAAGATGATGTGCATGTCCTTCATGGAAGGACCTTGACCACTCCTGTAGAGTTGATTAAATGTCTTAGGTCAATAAAAGAGCACGCCTTTGTTGCATCACAGTATCCTGTGATAATAACATTGGAGGATCACCTTACTCGAGATCTCCAAGCCAA
AGCAGCTGAGATGATCACCAAAACCTTTGGCGATTTGCTCTACTACCCCAAATCAGAAGTCTTAGAAGAGTTCCCTTCACCAGAATTCTTGAAGGGTCGCATAATCATCTCAACTAAACCTCCAAAGGAGTACTTAGAAGTTAAAACCACTGAAGTCCAGGGAAGTGGGGACAAGGAGAAGCTCAAGACACCTGATGAAG
AGCCGTGGGGTGATGACATCCCGGATTATGGCTCTGATGTGCTAGGCGAACGGGAGAGTGCAAATGTTGCTGGACACATTGAAGAAGATTCTGATGATGATGAAAGCATTTCACAAGCGAATCAAGATAAAGATGTACCACCTGAGTATAAGCGCCTTATAACCATTAGAGCAGGGAAGCCAAAAGGAGTGTCTTTGAAA
GATGCTATAAGAGTTGACAGTAAGCAGGTCAAGCGGGTTAGTTTAAGTGAACCACAACTTCAAAAGGTCTCTAGGTCTCATCCAACCCATATTGTTAGATTTACCCACAATAACTTCTTGAGAATATATCCAAAGGGTACTCGTATTGATTCATCAAACTATAACCCTTTGGTTGCTTGGACTCATGGAGCTCAGATGGT
GGCCTTAAATATGCAGGGATATGGAAGGCAGCTTTGGTTAGTGCAGGGTTTCTTTAGAGCCAATGGGGGCTGCGGTTATGTCAAAAAGCCTGACTTTCTTCTTTCAAACGGATCTAAAGATGATGACCAACATATTTTTAATCCAAAGACACCGGGAGCAGTCAAGACTACACTGAAGGTCAATATATATATGGGAGATG
GATGGAATCAGGATTTCAAGAAAACACACTTTGACACCTATTCTCCGCCGGACTTTTACAGCAGGGTTGGGATTGCAGGTGTGCCTGCAGATACTATCATGAAAAAGACAAGGGCGATAGAAGATGACTGGGTTCCGTGTTGGAATGAGGAGTTTGTTTTTCCATTGACTGTCCCTGAATTAGCTTTGCTTCGTATTGAA
GTGCACGAGTATGATATGTCTGAGAAAGATGACTTTGGTGGCCAGACATGCTTACCTGTGGCAGAGTTGAAGTCAGGGATTCGCTCAATTCCGCTATATGATAAGAAAGGAGAAAAATACAAGTCTGTAAGACTTTTGGTGGGAATCTCTAAGACTTGATTTCTACTAGCAATCATTCCTGCCGGGATAAGCGTCTTGTA
ATATATATCTATGAATCCAATTTGGGATTCTGGTTTAGTTTGGATTTTGGCAGGGAAAGTCTGTCTTGTCTATTTCTACAGAGGATATTTGGTAGCCAAAATTGAGTCTGAAACACTCACTTTCTGATGTGATTACTGATGGTGGGAATCTCTAAGACTTATGGTGCGAATTGATTTCTACTAGCAATAATTCCTGCAGG
GATAAAAGACTTGTAATATATATCTGTGAATCAAGTTTTGGGATTCTGGTTTAGTTTGAATTTTGGCATGGGAGATCTAATTATCTTTGTCTACCTTTATTAATACGTTTTTGTAATAATTCTATTTTGTAACGTAGGATACAAATGTCTTTCGGAGATATTTTAGAATTCCTACAAAAAGATGTTTGGTAGCCAAAATT
GAGTCTGCAGCATTCACTTTATGACGTGACTATTTAACCATCTTTCTCAGGGAGTATATATTACAATGGATGTTCATGAAATAGCAATGAACAGTACATTTGGATTGAATTAATTTTCAGTAATTACTCGAGACTCGTACAATTCTAAGGATATCAGTACAACAAACAAAAC

>TRINITY_DN115892_c1_g1_i1 from sample A1 / Example unspliced isoform:

CTGATGTGCTAGGCGAACGGGAGgtgataatatatactcaattcaagtcttttcgtgggcactcttatctacttgtccatactttcctacgttgttgatatggatccctttatccagaaaaagtcaatgcagcgtgtgcagttgcattctagctggattttaaatttccgcctccctcttgctgtcgattctaagatctt
aaagtctgtactggcagAGTGCAAATGTTGCTGGACACATTGAAGAAGATTCTGATGATGATGAAAGCATTTCACAAGCGAATCAAGATAAAGATGTACCACCTGAGTATAAGCGCCTTATAACCATTAGAGCAGGGAAGCCAAAAGGAGTGTCTTTGAAAGATGCTATAAGAGTTGACAGTAAGCAGGTCAAGCGGGTT
AGTTTAAGTGAACCACAACTTCAAAAGGTCTCTAGGTCTCATCCAACCCATATTGTTAGATTTACCCACAATAACTTCTTGAGAATATATCCAAAGGGTACTCGTATTGATTCATCAAACTATAACCCTTTGGTTGCTTGGACTCATGGAGCTCAGATGGTGGCCTTAAATATGCAGGGATATGGAAGGCAGCTTTGGTT
AGTGCAGGGTTTCTTTAGAGCCAATGGGGGCTGCGGTTATGTCAAAAAGCCTGACTTTCTTCTTTCAAACGGATCTAAAGATGATGACCAACATATTTTTAATCCAAAGACACCGGGAGCAGTCAAGACTACACTGAAGGTCAATATATATATGGGAGATGGATGGAATCAGGATTTCAAGAAAACACACTTTGACACCT
ATTCTCCGCCGGACTTTTACAGCAGGGTTGGGATTGCAGGTGTGCCTGCAGATACTATCATGAAAAAGACAAGGGCGATAGAAGATGACTGGGTTCCGTGTTGGAATGAGGAGTTTGTTTTTCCATTGACTGTCCCTGAATTAGCTTTGCTTCGTATTGAAGTGCACGAGTATGATATGTCTGAGAAAGATGACTTTGGT
GGCCAGACATGCTTACCTGTGGCAGAGTTGAAGTCAGGGATTCGCTCAATTCCGCTATATGATAAGAAAGGAGAAAAATACAAGTCTGTAAGACTTTTGGTGGGAATCTCTAAGACTTGATTTCTACTAGCAATCATTCCTGCCGGGATAAGCGTCTTGTAATATATATCTATGAATCCAATTTGGGATTCTGGTTTAGT
TTGGATTTTGGCAGGGAAAGTCTGTCTTGTCTATTTCTACAGAGGATATTTGGTAGCCAAAATTGAGTCTGAAACACTCACTTTCTGATGTGATTACTGATGGTGGGAATCTCTAAGACTTATGGTGCGAATTGATTTCTACTAGCAATAATTCCTGCAGGGATAAAAGACTTGTAATATATATCTGTGAATCAAGTTTT
GGGATTCTGGTTTAGTTTGAATTTTGGCATGGGAGATCTAATTATCTTTGTCTACCTTTATTAATACGTTTTTGTAATAATTCTATTTTGTAACGTAGGATACAAATGTCTTTCGGAGATATTTTAGAATTCCTACAAAAAGATGTTTGGTAGCCAAAATTGAGTCTGCAGCATTCACTTTATGACGTGACTATTTAACC
ATCTTTCTCAGGGAGTATATATTACAATGGATGTTCATGAAATAGCAATGAACAGTACATTTGGATTGAATTAATTTTCAGTAATTACTCGAGACTCGTACAATTCTAAGGATATCAGTACAACAAACAAAAC

Intron 4 GTGATAATAT ... CTTTATCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.06	75	52.3	9
A2	34.75	55	37	17
A3	40.13	66	56	18
A4	35.9	48	33.3	8
A5	34.92	20	13.7	8
A6	34.07	40	26	11
A7	34.08	153	102.7	27
A8	37.08	75	56	21
A9	35.53	137	96	34
A10	34.37	126	82	20.7

>TRINITY_DN115892_c1_g1_i3 from sample A1 / Example spliced isoform

AAAAGGATAAATTTTGCCTTGATGCGTCCAAATGGTTCTTCCGCCTGCACCAGATTTTGCAGTCGGTGACCAGTAGGTTTAAGCAAATATGGAATTAATTATCATTTATTTATTATACTGTATAGTACGTGTAAAATCCGCGTGCCCTTGACACGAAAACCCAACAAATTCGGACCATGACTCGGCTGTTCGAAGCATTT
GAATAGGCGTTGTGCCCACGCAGTCCAGCCTTCTGCTATATACATTGCATCACAGTGTTCCTGTGACCGCGGTAGGCAACGATAAGGTGCAGAATCGAACCCGCGACGAATGGGGAGCTACAGGGTATGCATCTGCTTCACCCGCAAGTTCAAATCTGGCGCCCTGAGCCCGCCCAAGGATGTCAAGGATCTGTTCGACA
AGTACTCGGAGAGCGGCGGGCAGATGAGCGTCAGGAATCTCCAGCGATTTCTCGTCGAAGTTCAGGGGCAGAACGACGCCACCGAGAAGGACGCCGAGAATGTCATCGAAGTGCTGCGGCAGCGCAGGCATGTGAGCGTCTTCCACCGTCACGGTATCAATCTCGACGGATTCTTCCATTTTCTCTTCAGCGACGATCTT
AACCCGGTCATCGATTCTAAGGTCCACCATGATATGACAGCACCATTGTCTCACTACTTCATATATACAGGTCATAATTCATATCTAACTGGAAATCAGCTGAGCAGTGACTGTAGCGATGCACCTATCATCAAAGCTCTTAAGAGAGGTGTACGGGTCATAGAGTTGGATATTTGGCCAAATTCAGCAAAAGATGATGT
GCATGTCCTTCATGGAAGGACCTTGACCACTCCTGTAGAGTTGATTAAATGTCTTAGGTCAATAAAAGAGCACGCCTTTGTTGCATCACAGTATCCTGTGATAATAACATTGGAGGATCACCTTACTCGAGATCTCCAAGCCAAAGCAGCTGAGATGATCACCAAAACCTTTGGCGATTTGCTCTACTACCCCAAATCAG
AAGTCTTAGAAGAGTTCCCTTCACCAGAATTCTTGAAGGGTCGCATAATCATCTCAACTAAACCTCCAAAGGAGTACTTAGAAGTTAAAACCACTGAAGTCCAGGGAAGTGGGGACAAGGAGAAGCTCAAGACACCTGATGAAGAGCCGTGGGGTGATGACATCCCGGATTATGGCTCTGATGTGCTAGGCGAACGGGAG


AAAAAGTCAATGCAGCGTGTGCAGTTGCATTCTAGCTGGATTTTAAATTTCCGCCTCCCTCTTGCTGTCGATTCTAAGATCTTAAAGTCTGTACTGGCAGAGTGCAAATGTTGCTGGACACATTGAAGAAGATTCTGATGATGATGAAAGCATTTCACAAGCGAATCAAGATAAAGATGTACCACCTGAGTATAAGCGCC
TTATAACCATTAGAGCAGGGAAGCCAAAAGGAGTGTCTTTGAAAGATGCTATAAGAGTTGACAGTAAGCAGGTCAAGCGGGTTAGTTTAAGTGAACCACAACTTCAAAAGGTCTCTAGGTCTCATCCAACCCATATTGTTAGATTTACCCACAATAACTTCTTGAGAATATATCCAAAGGGTACTCGTATTGATTCATCA
AACTATAACCCTTTGGTTGCTTGGACTCATGGAGCTCAGATGGTGGCCTTAAATATGCAGGGATATGGAAGGCAGCTTTGGTTAGTGCAGGGTTTCTTTAGAGCCAATGGGGGCTGCGGTTATGTCAAAAAGCCTGACTTTCTTCTTTCAAACGGATCTAAAGATGATGACCAACATATTTTTAATCCAAAGACACCGGG
AGCAGTCAAGACTACACTGAAGGTCAATATATATATGGGAGATGGATGGAATCAGGATTTCAAGAAAACACACTTTGACACCTATTCTCCGCCGGACTTTTACAGCAGGGTTGGGATTGCAGGTGTGCCTGCAGATACTATCATGAAAAAGACAAGGGCGATAGAAGATGACTGGGTTCCGTGTTGGAATGAGGAGTTTG
TTTTTCCATTGACTGTCCCTGAATTAGCTTTGCTTCGTATTGAAGTGCACGAGTATGATATGTCTGAGAAAGATGACTTTGGTGGCCAGACATGCTTACCTGTGGCAGAGTTGAAGTCAGGGATTCGCTCAATTCCGCTATATGATAAGAAAGGAGAAAAATACAAGTCTGTAAGACTTTTGGTGGGAATCTCTAAGACT
TGATTTCTACTAGCAATCATTCCTGCCGGGATAAGCGTCTTGTAATATATATCTATGAATCCAATTTGGGATTCTGGTTTAGTTTGGATTTTGGCAGGGAAAGTCTGTCTTGTCTATTTCTACAGAGGATATTTGGTAGCCAAAATTGAGTCTGAAACACTCACTTTCTGATGTGATTACTGATGGTGGGAATCTCTAAG
ACTTATGGTGCGAATTGATTTCTACTAGCAATAATTCCTGCAGGGATAAAAGACTTGTAATATATATCTGTGAATCAAGTTTTGGGATTCTGGTTTAGTTTGAATTTTGGCATGGGAGATCTAATTATCTTTGTCTACCTTTATTAATACGTTTTTGTAATAATTCTATTTTGTAACGTAGGATACAAATGTCTTTCGGA
GATATTTTAGAATTCCTACAAAAAGATGTTTGGTAGCCAAAATTGAGTCTGCAGCATTCACTTTATGACGTGACTATTTAACCATCTTTCTCAGGGAGTATATATTACAATGGATGTTCATGAAATAGCAATGAACAGTACATTTGGATTGAATTAATTTTCAGTAATTACTCGAGACTCGTACAATTCTAAGGATATCA
GTACAACAAACAAAAC

>TRINITY_DN115892_c1_g1_i1 from sample A1 / Example unspliced isoform:

CTGATGTGCTAGGCGAACGGGAGgtgataatatatactcaattcaagtcttttcgtgggcactcttatctacttgtccatactttcctacgttgttgatatggatccctttatccagAAAAAGTCAATGCAGCGTGTGCAGTTGCATTCTAGCTGGATTTTAAATTTCCGCCTCCCTCTTGCTGTCGATTCTAAGATCTT
AAAGTCTGTACTGGCAGAGTGCAAATGTTGCTGGACACATTGAAGAAGATTCTGATGATGATGAAAGCATTTCACAAGCGAATCAAGATAAAGATGTACCACCTGAGTATAAGCGCCTTATAACCATTAGAGCAGGGAAGCCAAAAGGAGTGTCTTTGAAAGATGCTATAAGAGTTGACAGTAAGCAGGTCAAGCGGGTT
AGTTTAAGTGAACCACAACTTCAAAAGGTCTCTAGGTCTCATCCAACCCATATTGTTAGATTTACCCACAATAACTTCTTGAGAATATATCCAAAGGGTACTCGTATTGATTCATCAAACTATAACCCTTTGGTTGCTTGGACTCATGGAGCTCAGATGGTGGCCTTAAATATGCAGGGATATGGAAGGCAGCTTTGGTT
AGTGCAGGGTTTCTTTAGAGCCAATGGGGGCTGCGGTTATGTCAAAAAGCCTGACTTTCTTCTTTCAAACGGATCTAAAGATGATGACCAACATATTTTTAATCCAAAGACACCGGGAGCAGTCAAGACTACACTGAAGGTCAATATATATATGGGAGATGGATGGAATCAGGATTTCAAGAAAACACACTTTGACACCT
ATTCTCCGCCGGACTTTTACAGCAGGGTTGGGATTGCAGGTGTGCCTGCAGATACTATCATGAAAAAGACAAGGGCGATAGAAGATGACTGGGTTCCGTGTTGGAATGAGGAGTTTGTTTTTCCATTGACTGTCCCTGAATTAGCTTTGCTTCGTATTGAAGTGCACGAGTATGATATGTCTGAGAAAGATGACTTTGGT
GGCCAGACATGCTTACCTGTGGCAGAGTTGAAGTCAGGGATTCGCTCAATTCCGCTATATGATAAGAAAGGAGAAAAATACAAGTCTGTAAGACTTTTGGTGGGAATCTCTAAGACTTGATTTCTACTAGCAATCATTCCTGCCGGGATAAGCGTCTTGTAATATATATCTATGAATCCAATTTGGGATTCTGGTTTAGT
TTGGATTTTGGCAGGGAAAGTCTGTCTTGTCTATTTCTACAGAGGATATTTGGTAGCCAAAATTGAGTCTGAAACACTCACTTTCTGATGTGATTACTGATGGTGGGAATCTCTAAGACTTATGGTGCGAATTGATTTCTACTAGCAATAATTCCTGCAGGGATAAAAGACTTGTAATATATATCTGTGAATCAAGTTTT
GGGATTCTGGTTTAGTTTGAATTTTGGCATGGGAGATCTAATTATCTTTGTCTACCTTTATTAATACGTTTTTGTAATAATTCTATTTTGTAACGTAGGATACAAATGTCTTTCGGAGATATTTTAGAATTCCTACAAAAAGATGTTTGGTAGCCAAAATTGAGTCTGCAGCATTCACTTTATGACGTGACTATTTAACC
ATCTTTCTCAGGGAGTATATATTACAATGGATGTTCATGAAATAGCAATGAACAGTACATTTGGATTGAATTAATTTTCAGTAATTACTCGAGACTCGTACAATTCTAAGGATATCAGTACAACAAACAAAAC