sp|Q9SAR5|AKR2A_ARATH ----> Ankyrin repeat domain-containing protein 2A

Intron 1 GTAAGTGTTT ... TCTACTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	6	6	1
A2	42.22	6	5	2.3
A3	50	4.7	4.7	0
A4	50	2	2	1.7
A5	100	0	2	1
A6	43.33	19	16	6
A7	62.34	23.7	32.7	9.7
A8	48.46	14	13.7	1
A9	49.85	32	31	15.3
A10	46.97	12.7	10.7	3.3

>TRINITY_DN101064_c0_g3_i4 from sample A4 / Example spliced isoform

TGGAAGGCCGGCTCTTCATCAGCAATTACAAGCAACAACAGCAGCCATGGCATCTGCATCAGCTCCCTCAGGCAAGCCTTTTCGGTGGTGGCGAGAGCATACTTTGAGAGAGCAATGCTGGATTCGTTTATTCAACTTGCAAAAAGGAAATGAAAGGTGTGACTAATTACTACACTACGAAGCTCCGCCCCTTGCTCCAA
AAAGACAACACAAAAGAAGCCTCAAGCACGTCTACTGAGGCTTCAGTCAGTCCATCTACAGTTGCTGGTGACATTGCAGACAATGCAGGAGGAAATAGACAAACAGGCAATCAAGACAATGCAACATTGCCTAGCCCTTTTGACTTATCTTCAATGTCGAGCCTTTTAAATGATCCCAGCATAAAAGAGTTGGCAAAAGA
TGTTTCCAAGGATCCAGCATTTCGGAAGATGGCTCAACAACTTCAGCAAAGTGTGAAGGGTGCTGGTCATTCTGCTACACCCCAACTAGATCCAGAAAAGTATGTCAAGAGCATGCAACAGGTTATGCAAAATCCTCAGTTCATGACAATGGCACAACACCTTCGCCATTCTCTGATGCAGGATCCAGCAGTAATGAATA
TGATTCAGACCTTGTCAAACCCTACACAAAAAATGCAATTTGAAGCACAGTTGGCCCAGCTTAAGAATGATCCTTCTCTAAAGCCTGTTTTGGACGAAATTTCAAAGGGCGGACCTCCTGCAATAATGAAGTATTGGAATGATCCGGTGGTGCTCAGTAAACTTGGGAAAGCTTTCCAAATGGGAAGCTCAGGAGATATG
CCAGTTCCCAAGCAGTCTGCTAAAAATAAAAGAGAAGAAACTAATGAAGACACTGAAAAGGAGACATCTTTGACTTTGACTCATGCAGCCAGCACTGGCGATGTTAAGGAGCTGCAAGGTTTGCTTGCAAGGGGTGTAGACAAAGATAAGAAAGATTCACAGGGAAGAACAGCCTTACATTTTGCATGTGGATATGGAAA
TGCAAGGTGTGTAGAAGTGTTGCTTGAAGCAGCTGCTTCAGTTGATCCTCTTGATAAGAACAACAACACTCCTCTTCATTATGCTTCAGTTGATCCTCTTGATAAGAACAACAACACTCCTCTTCATTATGCTTCAGGTTATGGACAATATGAATGTGCTGAACTTCTACTCAAGGGAGGAGCTGCAGTCCAAAGAGGAT
GAAACAGCAATTTCCAGGGCACAATTGTTCATATTGTTTGGCTATTGCTCAGATCGATTTTGACTGTTTAGCATAGAGCTGAGCTTTATTGATTTTGTGTTGACAGAACTCTGGTGAACTTGGAAGGGAAAACACCAATGGATGTTGCAAAGCTTAATAAGCGCATGCAAGTCATAAAGTTGCTTGAGAAAAATGCATTT
TGTAGTTGATGTAAGATGCTCAAGATAAATCTGGTAGGTGACATAAAGAATAATATGGCCAATTTGAGCAGTTTTCTGTAAATTAAGATGTGTTGATGCCATTTAAGTAGGCTGGGATCAAACTTGCTACTTTGATCTATCACTCAAAGTATCTGTTGTTAAAATTAGAAGTTTGTGTTGCTCATTTTAACTAATTTTAA
GAGTTTTCTCTTGCTCGTGGAGAATCTAAAAATACAG

>TRINITY_DN101064_c0_g3_i1 from sample A4 / Example unspliced isoform:

TGGAAGGCCGGCTCTTCATCAGCAATTACAAGCAACAACAGCAGCCATGGCATCTGCATCAGCTCCCTCAGACAACACAAAAGAAGCCTCAAGCACGTCTACTGAGGCTTCAGTCAGTCCATCTACAGTTGCTGGTGACATTGCAGACAATGCAGGAGGAAATAGACAAACAGGCAATCAAGACAATGCAACATTGCCTA
GCCCTTTTGACTTATCTTCAATGTCGAGCCTTTTAAATGATCCCAGCATAAAAGAGTTGGCAAAAGATGTTTCCAAGGATCCAGCATTTCGGAAGATGGCTCAACAACTTCAGCAAAGTGTGAAGGGTGCTGGTCATTCTGCTACACCCCAACTAGATCCAGAAAAGTATGTCAAGAGCATGCAACAGGTTATGCAAAAT
CCTCAGTTCATGACAATGGCACAACACCTTCGCCATTCTCTGATGCAGGATCCAGCAGTAATGAATATGATTCAGACCTTGTCAAACCCTACACAAAAAATGCAATTTGAAGCACAGTTGGCCCAGCTTAAGAATGATCCTTCTCTAAAGCCTGTTTTGGACGAAATTTCAAAGGGCGGACCTCCTGCAATAATGAAGTA
TTGGAATGATCCGGTGGTGCTCAGTAAACTTGGGAAAGCTTTCCAAATGGGAAGCTCAGGAGATATGCCAGTTCCCAAGCAGTCTGCTAAAAATAAAAGAGAAGAAACTAATGAAGACACTGAAAAGGAGACATCTTTGACTTTGACTCATGCAGCCAGCACTGGCGATGTTAAGGAGCTGCAAGGTTTGCTTGCAAGGG
GTGTAGACAAAGATAAGAAAGATTCACAGGGAAGAACAGCCTTACATTTTGCATGTGGATATGGAAATGCAAGGTGTGTAGAAGTGTTGCTTGAAGCAGCTGCTTCAGTTGATCCTCTTGATAAGAACAACAACACTCCTCTTCATTATGCTTCAGTTGATCCTCTTGATAAGAACAACAACACTCCTCTTCATTATGCT
TCAGGTTATGGACAATATGAATGTGCTGAACTTCTACTCAAGGGAGGAGCTGCAGTgtaagtgtttccatctttctcttttggttgttagtctgcctaatttctaatttgtctttgcaagaaaccagagcaatgacttgaagcatgcaaaaggtctttgatgagaccattattgtgggtttctactacagCCAAAGAGGA
TGAAACAGCA

Intron 2 GTAAGTGTTT ... GTGTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	7.14	13	5.9	1
A2	34.78	15	5.9	8
A3	23.08	10	5.1	3
A4	66.67	2	1.7	4
A5	33.33	2	2	1
A6	27.94	49	19.2	19
A7	36.19	67	36.3	38
A8	29.41	48	14.8	20
A9	33.75	53	34.8	27
A10	29.41	36	12.9	15

>TRINITY_DN44681_c1_g4_i1 from sample A7 / Example spliced isoform

ATCACCGAAAAGATAACGTGGATAATCTCTCCTTCCATTATTTTGCAGTTTTAAACCTCTGACTGAACCCAATGGAAGGCCGGCTCTTCATCAGCAATTACAAGCAACAACAGCAGCCATGGCATCTGCATCAGCTCCCTCAGGCAAGCCTTTTCGGTGGTGGCGAGAGCATACTTTGAGAGAGCAATGCTGGATTCGTT
TATTCAACTTGCAAAAAGGAAATGAAAGGTGTGACTAATTACTACACTACGAAGCTCCGCCCCTTGCTCCAAAAAGACAACACAAAAGAAGCCTCAAGCACGTCTACTGAGGCTTCAGTCAGTCCATCTACAGTTGCTGGTGACATTGCAGACAATGCAGGAGGAAATAGACAAACAGGCAATCAAGACAATGCAACATT
GCCTAGCCCTTTTGACTTATCTTCAATGTCGAGCCTTTTAAATGATCCCAGCATAAAAGAGTTGGCAAAAGATGTTTCCAAGGATCCAGCATTTCGGAAGATGGCTCAACAACTTCAGCAAAGTGTGAAGGGTGCTGGTCATTCTGCTACACCCCAACTAGATCCAGAAAAGTATGTCAAGAGCATGCAACAGGTTATGC
AAAATCCTCAGTTCATGACAATGGCACAACACCTTCGCCATTCTCTGATGCAGGATCCAGCAGTAATGAATATGATTCAGACCTTGTCAAACCCTACACAAAAAATGCAATTTGAAGCACAGTTGGCCCAGCTTAAGAATGATCCTTCTCTAAAGCCTGTTTTGGACGAAATTTCAAAGGGCGGACCTCCTGCAATAATG
AAGTATTGGAATGATCCGGTGGTGCTCAGTAAACTTGGGAAAGCTTTCCAAATGGGAAGCTCAGGAGATATGCCAGTTCCCAAGCAGTCTGCTAAAAATAAAAGAGAAGAAACTAATGAAGACACTGAAAAGGAGACATCTTTGACTTTGACTCATGCAGCCAGCACTGGCGATGTTAAGGAGCTGCAAGGTTTGCTTGC
AAGGGGTGTAGACAAAGATAAGAAAGATTCACAGGGAAGAACAGCCTTACATTTTGCATGTGGATATGGAAATGCAAGGTGTGTAGAAGTGTTGCTTGAAGCAGCTGCTTCAGTTGATCCTCTTGATAAGAACAACAACACTCCTCTTCATTATGCTTCAGGTTATGGACAATATGAATGTGCTGAACTTCTACTCAAGG
GAGGAGCTGCAGTAACTCTGGTGAACTTGGAAGGGAAAACACCAATGGATGTTGCAAAGCTTAATAAGCGCATGCAAGTCATAAAGTTGCTTGAGAAAAATGCATTTTGTAGTTGATGTAAGATGCTCAAGATAAATCTGGTAGGTGACATAAAGAATAATATGGCCAATTTGAGCAGTTTTCTGTAAATTAAGATGTGT
TGATGCCATTTAAGTAGGCTGGGATCAAACTTGCTACTTTGATCTATCACTCAAAGTATCTGTTGTTAAAATTAGAAGTTTGTGTTGCTCATTTTAACTAATTTTAAGAGTTTTCTCTTGCTCGTGGAGAATCTAAAAATACAGTTGCACCTTTCTAATTTACAGCAGCAACTATTTTTATCTATGCTCTAGAAAAGCAC
ACAGTTTTCTAAGCTGGCTTCCAAATTTTAGAAATTTACAGCATTGAAAACTTTGAAAGATTGGGTTCCCT

>TRINITY_DN44681_c1_g4_i5 from sample A7 / Example unspliced isoform:

ATCACCGAAAAGATAACGTGGATAATCTCTCCTTCCATTATTTTGCAGTTTTAAACCTCTGACTGAACCCAATGGAAGGCCGGCTCTTCATCAGCAATTACAAGCAACAACAGCAGCCATGGCATCTGCATCAGCTCCCTCAGACAACACAAAAGAAGCCTCAAGCACGTCTACTGAGGCTTCAGTCAGTCCATCTACAG
TTGCTGGTGACATTGCAGACAATGCAGGAGGAAATAGACAAACAGGCAATCAAGACAATGCAACATTGCCTAGCCCTTTTGACTTATCTTCAATGTCGAGCCTTTTAAATGATCCCAGCATAAAAGAGTTGGCAAAAGATGTTTCCAAGGATCCAGCATTTCGGAAGATGGCTCAACAACTTCAGCAAAGTGTGAAGGGT
GCTGGTCATTCTGCTACACCCCAACTAGATCCAGAAAAGTATGTCAAGAGCATGCAACAGGTTATGCAAAATCCTCAGTTCATGACAATGGCACAACACCTTCGCCATTCTCTGATGCAGGATCCAGCAGTAATGAATATGATTCAGACCTTGTCAAACCCTACACAAAAAATGCAATTTGAAGCACAGTTGGCCCAGCT
TAAGAATGATCCTTCTCTAAAGCCTGTTTTGGACGAAATTTCAAAGGGCGGACCTCCTGCAATAATGAAGTATTGGAATGATCCGGTGGTGCTCAGTAAACTTGGGAAAGCTTTCCAAATGGGAAGCTCAGGAGATATGCCAGTTCCCAAGCAGTCTGCTAAAAATAAAAGAGAAGAAACTAATGAAGACACTGAAAAGG
AGACATCTTTGACTTTGACTCATGCAGCCAGCACTGGCGATGTTAAGGAGCTGCAAGGTTTGCTTGCAAGGGGTGTAGACAAAGATAAGAAAGATTCACAGGGAAGAACAGCCTTACATTTTGCATGTGGATATGGAAATGCAAGGTGTGTAGAAGTGTTGCTTGAAGCAGCTGCTTCAGTTGATCCTCTTGATAAGAAC
AACAACACTCCTCTTCATTATGCTTCAGGTTATGGACAATATGAATGTGCTGAACTTCTACTCAAGGGAGGAGCTGCAGTgtaagtgtttccatctttctcttttggttgttagtctgcctaatttctaatttgtctttgcaagaaaccagagcaatgacttgaagcatgcaaaaggtctttgatgagaccattattgtg
ggtttctactacagccaaagaggatgaaacagcaatttccagggcacaattgttcatattgtttggctattgctcagatcgattttgactgtttagcatagagctgagctttattgattttgtgttgacagAACTCTGGTGAACTTGGAAGGGAAAACACCAATGGATGTTGCAAAGCTTAATAAGCGCATGCAAGTCAT
AAAGTTGCTTGAGAAAAATGCATTTTGTAGTTGATGTAAGATGCTCAAGATAAATCTGGTAGGTGACATAAAGAATAATATGGCCAATTTGAGCAGTTTTCTGTAAATTAAGATGTGTTGATGCCATTTAAGTAGGCTGGGATCAAACTTGCTACTTTGATCTATCACTCAAAGTATCTGTTGTTAAAATTAGAAGTTTG
TGTTGCTCATTTTAACTAATTTTAAGAGTTTTCTCTTGCTCGTGGAGAATCTAAAAATACAGTTGCACCTTTCTAATTTACAGCAGCAACTATTTTTATCTATGCTCTAGAAAAGCACACAGTTTTCTAAGCTGGCTTCCAAATTTTAGAAATTTACAGCATTGAAAACTTTGAAAGATTGGGTTCCCT

Intron 3 GCAAGCCTTT ... CTCCAAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.81	16	2	8
A2	45.45	6	3	7
A3	20	16	1	7
A4	36	8	2	7
A5	50	3	4	2
A6	42.86	2	3	0
A7	36.51	20	10	13
A8	18.92	15	3	4
A9	34.62	17	3	15
A10	37.93	18	6	16

>TRINITY_DN44681_c1_g4_i6 from sample A7 / Example spliced isoform

ATCACCGAAAAGATAACGTGGATAATCTCTCCTTCCATTATTTTGCAGTTTTAAACCTCTGACTGAACCCAATGGAAGGCCGGCTCTTCATCAGCAATTACAAGCAACAACAGCAGCCATGGCATCTGCATCAGCTCCCTCAGACAACACAAAAGAAGCCTCAAGCACGTCTACTGAGGCTTCAGTCAGTCCATCTACAG
TTGCTGGTGACATTGCAGACAATGCAGGAGGAAATAGACAAACAGGCAATCAAGACAATGCAACATTGCCTAGCCCTTTTGACTTATCTTCAATGTCGAGCCTTTTAAATGATCCCAGCATAAAAGAGTTGGCAAAAGATGTTTCCAAGGATCCAGCATTTCGGAAGATGGCTCAACAACTTCAGCAAAGTGTGAAGGGT
GCTGGTCATTCTGCTACACCCCAACTAGATCCAGAAAAGTATGTCAAGAGCATGCAACAGGTTATGCAAAATCCTCAGTTCATGACAATGGCACAACACCTTCGCCATTCTCTGATGCAGGATCCAGCAGTAATGAATATGATTCAGACCTTGTCAAACCCTACACAAAAAATGCAATTTGAAGCACAGTTGGCCCAGCT
TAAGAATGATCCTTCTCTAAAGCCTGTTTTGGACGAAATTTCAAAGGGCGGACCTCCTGCAATAATGAAGTATTGGAATGATCCGGTGGTGCTCAGTAAACTTGGGAAAGCTTTCCAAATGGGAAGCTCAGGAGATATGCCAGTTCCCAAGCAGTCTGCTAAAAATAAAAGAGAAGAAACTAATGAAGACACTGAAAAGG
AGACATCTTTGACTTTGACTCATGCAGCCAGCACTGGCGATGTTAAGGAGCTGCAAGGTTTGCTTGCAAGGGGTGTAGACAAAGATAAGAAAGATTCACAGGGAAGAACAGCCTTACATTTTGCATGTGGATATGGAAATGTAATGAGCCCCATAGCATCCCATGAACCTATTTTTCGAATGAGTATGAAAGTCTTTGCT
TGGTTTTGGGTTTTATAATTTAGTTATTGCCTATTTTTGATAGCTGATTTACAGCTGTAGCTGTATTGAAAAGAGCATTATATTAAAATGCATTATTTTTTAAAAAACAAAACCATTATTTTAAAACTACTTTTTG

>TRINITY_DN44681_c1_g4_i1 from sample A7 / Example unspliced isoform:

ATCACCGAAAAGATAACGTGGATAATCTCTCCTTCCATTATTTTGCAGTTTTAAACCTCTGACTGAACCCAATGGAAGGCCGGCTCTTCATCAGCAATTACAAGCAACAACAGCAGCCATGGCATCTGCATCAGCTCCCTCAGgcaagccttttcggtggtggcgagagcatactttgagagagcaatgctggattcgtt
tattcaacttgcaaaaaggaaatgaaaggtgtgactaattactacactacgaagctccgccccttgctccaaaaagACAACACAAAAGAAGCCTCAAGCACGTCTACTGAGGCTTCAGTCAGTCCATCTACAGTTGCTGGTGACATTGCAGACAATGCAGGAGGAAATAGACAAACAGGCAATCAAGACAATGCAACATT
GCCTAGCCCTTTTGACTTATCTTCAATGTCGAGCCTTTTAAATGATCCCAGCATAAAAGAGTTGGCAAAAGATGTTTCCAAGGATCCAGCATTTCGGAAGATGGCTCAACAACTTCAGCAAAGTGTGAAGGGTGCTGGTCATTCTGCTACACCCCAACTAGATCCAGAAAAGTATGTCAAGAGCATGCAACAGGTTATGC
AAAATCCTCAGTTCATGACAATGGCACAACACCTTCGCCATTCTCTGATGCAGGATCCAGCAGTAATGAATATGATTCAGACCTTGTCAAACCCTACACAAAAAATGCAATTTGAAGCACAGTTGGCCCAGCTTAAGAATGATCCTTCTCTAAAGCCTGTTTTGGACGAAATTTCAAAGGGCGGACCTCCTGCAATAATG
AAGTATTGGAATGATCCGGTGGTGCTCAGTAAACTTGGGAAAGCTTTCCAAATGGGAAGCTCAGGAGATATGCCAGTTCCCAAGCAGTCTGCTAAAAATAAAAGAGAAGAAACTAATGAAGACACTGAAAAGGAGACATCTTTGACTTTGACTCATGCAGCCAGCACTGGCGATGTTAAGGAGCTGCAAGGTTTGCTTGC
AAGGGGTGTAGACAAAGATAAGAAAGATTCACAGGGAAGAACAGCCTTACATTTTGCATGTGGATATGGAAATGCAAGGTGTGTAGAAGTGTTGCTTGAAGCAGCTGCTTCAGTTGATCCTCTTGATAAGAACAACAACACTCCTCTTCATTATGCTTCAGGTTATGGACAATATGAATGTGCTGAACTTCTACTCAAGG
GAGGAGCTGCAGTAACTCTGGTGAACTTGGAAGGGAAAACACCAATGGATGTTGCAAAGCTTAATAAGCGCATGCAAGTCATAAAGTTGCTTGAGAAAAATGCATTTTGTAGTTGATGTAAGATGCTCAAGATAAATCTGGTAGGTGACATAAAGAATAATATGGCCAATTTGAGCAGTTTTCTGTAAATTAAGATGTGT
TGATGCCATTTAAGTAGGCTGGGATCAAACTTGCTACTTTGATCTATCACTCAAAGTATCTGTTGTTAAAATTAGAAGTTTGTGTTGCTCATTTTAACTAATTTTAAGAGTTTTCTCTTGCTCGTGGAGAATCTAAAAATACAGTTGCACCTTTCTAATTTACAGCAGCAACTATTTTTATCTATGCTCTAGAAAAGCAC
ACAGTTTTCTAAGCTGGCTTCCAAATTTTAGAAATTTACAGCATTGAAAACTTTGAAAGATTGGGTTCCCT