NA ----> NA

Intron 1 GTTGCTCATT ... CCGTAGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	56.52	15	20	19
A2	62.5	9	17	13
A3	57.75	15	24	17
A4	58.97	16	25	21
A5	64.71	3	6	5
A6	50	22	24	20
A7	54.72	12	18	11
A8	53.62	16	21	16
A9	54.55	20	27	21
A10	62.86	26	49	39

>TRINITY_DN40718_c1_g1_i1 from sample A6 / Example spliced isoform

AGGAACCCAATAACTGTCCATTCCTCTCTCCCCCAACTCAGTGGGTCGGTTGTGGTGCAAGTTTCTCTAGTCCGTAGGCATGTTCCTCATTCCGTCGAGCTAGGCTCTTGACTCTGCTCCAAAGGCTATTGGACGAGGATCGACCGTGGCCCTGCGGACTTTGTCGATCCTTTGGTTTCTTTCTTTCTTGGTTCTTGGAG
GGAAGGCGATATGAGGAATGCGATATGAGGAATGCTGGAGCAAATCGGTTCTCTGGAGGGAAGGCGAAGGAACCCAATAACTGTCCATTCCTCTCTTCCCCAACTCAGTGAGTCGGTTGTGGTGCAAGTTCCTCTAGTCCGTAGGCAGGTTGCTCATTCCGTCGAGCTAGGCTCTTGACTCTGCTCCAAAGGCTATTGGA
CGAGGATCGACCGTGGCCCTGCGGACTTTGTCGATCCTTTGGTTTCTTTCTTTCTTGGTTCTTGGAGGGAAGGCGATATGAGGAATGCGATATGAGGAATGCTGGAGCAAATCGGTTCTCGGGAGGGAAGTCGATATGACGAATCCTTACCCAAACGGAACAGGAAAGAAATCCGCTGGTTCATCTACCAGGATATTTCT
ATTTGCAAAGGATTTTTCTTATTTAATGGAGTTATTTATCATCATACTTTTACATTGTACTGATCTCCCTAGTAGGATATTGGACGAGGATCG

>TRINITY_DN40718_c1_g1_i2 from sample A6 / Example unspliced isoform:

AGGAACCCAATAACTGTCCATTCCTCTCTCCCCCAACTCAGTGGGTCGGTTGTGGTGCAAGTTTCTCTAGTCCGTAGGCATGTTCCTCATTCCGTCGAGCTAGGCTCTTGACTCTGCTCCAAAGGCTATTGGACGAGGATCGACCGTGGCCCTGCGGACTTTGTCGATCCTTTGGTTTCTTTCTTTCTTGGTTCTTGGAG
GGAAGGCGATATGAGGAATGCGATATGAGGAATGCTGGAGCAAATCGGTTCTCTGGAGGGAAGGCGAAGGAACCCAATAACTGTCCATTCCTCTCTTCCCCAACTCAGTGAGTCGGTTGTGGTGCAAGTTCCTCTAGTCCGTAGGCAGgttgctcattccgtcgagctaggctcttgactctgctccaaagtaggatatt
ggacgaggatcgaccgtggccctgcggactttgtcgatcctttggtttctttctttcttggttcttggagggaaggcgatatgaggaatgcgatatgaggaatgctggagcaaatcggttctctggagggaaggcgaaggaacccaataactgtccattcctctcttccccaactcagtgagtcggttgtggtgcaagtt
cctctagtccgtaggcagGTTGCTCATTCCGTCGAGCTAGGCTCTTGACTCTGCTCCAAAGGCTATTGGACGAGGATCGACCGTGGCCCTGCGGACTTTGTCGATCCTTTGGTTTCTTTCTTTCTTGGTTCTTGGAGGGAAGGCGATATGAGGAATGCGATATGAGGAATGCTGGAGCAAATCGGTTCTCGGGAGGGAAG
TCGATATGACGAATCCTTACCCAAACGGAACAGGAAAGAAATCCGCTGGTTCATCTACCAGGA

Intron 2 GTATTTCTAT ... TCCCTAGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.03	10	5	4
A2	50	8	6	10
A3	12.5	21	2	4
A4	36.59	13	8	7
A5	64.71	3	5	6
A6	10	27	4	2
A7	12.82	34	6	4
A8	10	18	4	0
A9	15.25	25	4	5
A10	23.46	31	13	6

>TRINITY_DN115827_c1_g2_i7 from sample A3 / Example spliced isoform

GTTGACCTCTAGTTTAGTTTTGGTTTTGTTTCCGCATAGGGGCTTCAGTTTACTTGGATTATGGTTTCAATGGTTGATAGTTTGATGATGTCTCCTCTTTATTCTTGCGAGGAACCCAATAACTGTCCATTCCTCTCTTCCCCAACTCAGTGGGTCGGTTGTGGTGCAAGTTTCTCTAGTCCGTAGGCATGTTCCTCATT
CCGTCGAGCTAGGCTCTTGACTCTGCTCCAAAGGATATTGGACGAGGATCGACCGTGGCCCTGCGGACTTTGTCGATCCTTTGGTTTCTTTCTTTCTTGGTTCTTGGAGGGAAGGCGATATGAGGAATGCGATATGAGGAATGCTGGAGCAAATCGGTTCTCTGGAGGGAAGGCGAAGGAACCCAATAACTGTCCATTCC
TCTCTTCCCCAACTCAGTGAGTCGGTTGTGGTGCAAGTTCCTCTAGTCCGTAGGCAGGTTGCTCATTCCGTCGAGCTAGGCTCTTGACTCTGCTCCAAAGGCTATTGGACGAGGATCGACCGTGGCCCTGCGGACTTTGTCGATCCTTTGGTTTCTTTCTTTCTTGGTTCTTGGAGGGAAGGCGATATGAGGAATGCGAT
A

>TRINITY_DN115827_c1_g2_i8 from sample A3 / Example unspliced isoform:

TCTTGCGAGGAACCCAATAACTGTCCATTCCTCTCTTCCCCAACTCAGTGGGTCGGTTGTGGTGCAAGTTTCTCTAGTCCGTAGGCATGTTCCTCATTCCGTCGAGCTAGGCTCTTGACTCTGCTCCAAAGgtatttctatttgcaaaggatttttcttatttaatggagttatttatcatcatacttttacattgtaca
ttgtactgatcccggtattgatctccctagtagGATATTGGACGAGGATCGACCGTGGCCCTGCGGACTTTGTCGATCCTTTGGTTTCTTTCTTTCTTGGTTCTTGGAGGGAAGGCGATATGAGGAATGCGATATGAGGAATGCTGGAGCAAATCGGTTCTCTGGAGGGAAGGCGATATGACGAATCCTGGTTCATCTAC
TAGGAGTAATAAGGAAGGTCGGTCGACCATAGATCCATTATTTTTATAGGCTGTAGGATTTATATACTGCACCATTGCATGAATTGTAAATTTCAGTTATTCTTGTAGGATTTATATACTTTCAATTTATAGTAACAAAAGATTTCCATCAAGACGATTCAGTGAATTAAATATACAAACATAAAAAATCTGGAAGGCTT
GAACAATCATAAATATAATTGAAATAGGAATTCTTTTAAGTGGGATTTATCAAGATCGTTGAAGTGAGGGTGATCTTTGGCCGTCTTTGTTGAGATAAACGTAGTAGAGACTCTGCAGAAGATCCCGGAGGAACAACGGAAAGAAATGAGGAAAACCATAATCCAGGATGCTTTTATGATTTCCTTCATAAATGGAGATG
GCAAACCATAAGCCTCACGATCTGCACAGTGCCACCGATAGAATTCAATAAAACAATTACAGCAAATACACAAAACATATATGAATAGCGTGAATAAGAAACAATTAAATTCAACATGTTTATGTTTAACTAATGATAAGTACCTCAATTAATGCTAATAAAGAACAGGTGAACATCGATCTAGTTTGCAGCTTCCTTAA
TGGGCCATCACATTTCTTCTCGGGGCAGAGTTTTTTCTTTGAGGGAATTAATGATTTTTCTGATCGACTTCGGGAACTCGAACTCTGAGTATCGGGCTGGAAATGACAGTTGTGAATAGATACTCAGAGTTCACTGCATTCTTGGCCATATTATATTATCAGCCATGGCATTCATTAGTCATATATTGATCGAGGAAAAA
AATCTGTACAAATGAGTTCACTACATTCATTGGTCATATTATATTATCAGCCATGGCATTCATTAGTCATATATTGATCGAGGAAAAAAATCTGGTTGCCACATTAGAATGTTCCATTAAATTGAATAGTGAAGAATTCCGAGGAATTGCAAGTAGAGTGGAAGCGGGCACCATAATAATTACCATCTGGCCATGAGATC
AGATGGTTCAACATCCCACAACAACGTAGTTTTTCAAGATATAGTTGATCTGTTATTCATCTTGCTAACGAGACCAAATGGATTTTCTTAGCTTTGGAAACCAGAAAGATGGGAAAGTTATCGACTATGTATGCAGTACAGTCTTATAGACTTGTTAGATAGCACAGTTATTTAATACAGACAAACAATAATTTCACAAA
TTTTTCTTAACTCATACGAATACCCACTCTTTAAGCCCATGTTTGTGCTATGCGGCCGGTACATACTTACCCACACCATCTATAGGCACCCTTAGGTTCTGACTAAGGGCAGGTATATATTCGGG

Intron 3 GTAACTCACA ... ACGCACGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.55	31	19	7
A2	8.71	22.7	4.3	0
A3	50	10	12	8
A4	16.08	15.7	5	1
A5	76.47	2	6	7
A6	36.36	7	6	2
A7	31.47	32.7	21	9
A8	27.78	26	15	5
A9	51.02	12	11	14
A10	38.55	18.3	6	17

>TRINITY_DN105408_c2_g3_i1 from sample A4 / Example spliced isoform

GCTAAATCTCAAACAGAAATTATCCTTTTTAAGGCATATATTTGGAAATTCATGTTAAAATGCTGTGTGACATTTAACCTCTTCGCTGCATATTTTTCCAGAGAATTAACGCACGCAGGTAACTCACAACACACCCAGCTACTAAGGTGTGCTAGTAGATGGTGGGAGATCAATTTTTAGAAAGGTATAATTATTTATAA
TTACTGGTGTACAGTGACCTAATTCATTTATATGTGGCAGCTAATTTGTCGTGGTGACATTGGCATACCTGGTGTAATTTTAGGAGGTATTGTTAAATTTATTGTATGTATTAGGTATTTACCCTATTAAAGGCACTGGTATCGACAATGGCATGCTAAATCTCAAACAGAAATTATCCTTTTTAAGGCATATATTTGGA
AATTCATGTTAAAATGCTGTGTGACATTTAACCTCTTCGCTGCATATTTTTCCAGAGAATTAACGCACGCAGGTAACTCACAACACACCCAGCAAAACCCAGCTACTAAGGTGTGCCAGTAGATGGTGGGGATCAATTTTTAGAAAGAGAATTAACGCACGCAGGTAACTCACAACACACCCAGCTACTAAGGTGTGCTA
GTAGATGGTGGGGATCAATTTTTAGAAAGAGAATTAACGCACGCAGGT

>TRINITY_DN105408_c2_g3_i1 from sample A4 / Example unspliced isoform:

GCTAAATCTCAAACAGAAATTATCCTTTTTAAGGCATATATTTGGAAATTCATGTTAAAATGCTGTGTGACATTTAACCTCTTCGCTGCATATTTTTCCAGAGAATTAACGCACGCAGGTAACTCACAACACACCCAGCTACTAAGGTGTGCTAGTAGATGGTGGGAGATCAATTTTTAGAAAGGTATAATTATTTATAA
TTACTGGTGTACAGTGACCTAATTCATTTATATGTGGCAGCTAATTTGTCGTGGTGACATTGGCATACCTGGTGTAATTTTAGGAGGTATTGTTAAATTTATTGTATGTATTAGGTATTTACCCTATTAAAGGCACTGGTATCGACAATGGCATGCTAAATCTCAAACAGAAATTATCCTTTTTAAGGCATATATTTGGA
AATTCATGTTAAAATGCTGTGTGACATTTAACCTCTTCGCTGCATATTTTTCCAGAGAATTAACGCACGCAGgtaactcacaacacacccagcaaaacccagctactaaggtgtgccagtagatggtggggatcaatttttagaaagagaattaacgcacgcagGTAACTCACAACACACCCAGCTACTAAGGTGTGCTA
GTAGATGGTGGGGATCAATTTTTAGAAAGAGAATTAACGCACGCAGGT

Intron 4 GTAAAAGGTT ... TCCCTAGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.23	7.9	4	5.8
A2	54.67	7	6	10.9
A3	22.95	19.6	5.9	5.8
A4	41.43	13.9	10.8	8.9
A5	68.01	3	4.1	8.7
A6	7.35	25.2	2	2
A7	13.7	35.4	7	4.2
A8	14.06	18	4.9	1
A9	19.35	22.9	4	7
A10	22.02	26.8	8.9	6

>TRINITY_DN122107_c0_g1_i7 from sample A1 / Example spliced isoform

CTCTCATTACCGTTAGATGTAGGTTTCTGAAAAAGCGAAAAACCTGCCTGTCCTTTTCGATGTCAGGAGGTGCGGAGCTATCGCTCACTCGTCTCTAACATCTCCACTACCTAACCTCACCACCTTCGATGATCGCGCAGTTACCTAACCACACCAGCTTCGATGATCGCGCAGTTAATGGATTATTGCTTCACTTGGAG
CTCATCCTGGATTATTCTAAACATTAACTTTACTGACTGCCATGGTGTTTTGGGCTCGAGATTAGGTTTTGGTATGCGATTTGGTCATTGTACATGGTTGACCTCTAGTTTAGTTCTGGTTTTGTTTCCGCACAGGGGCTTCAGTTTACTTGGATTATGGTTTCAATGTATGGTAGTTTGATGATGGCTCCTCTTTACTC
TTGCGAGGAACCCAATAACTGTCCATTCCTCTCTCCCCCAACTCAGTGGGTCGGTTGTGGTGCAAGTTTCTCTAGTCCGTAGGCATGTTCCTCATTCCGTCGAGCTAGGCTCTTGACTCTGCTCCAAAGGATATTGGACGAGGATCGACCGTGGCCCGGCGGACTTTGTCGATCCTTTGGTTTCTTTCTTTCTTGGTTCT
TGGAGGGAAGGCGATATGAGGAATGCGATATGAGGAATGCTGGAGCAAATCGGTTCTCTGGAGGGAAGGCGAAGGAACCCAATAACTGTCCATTCCTCTCTTCCCCAACTCAGTGAGTCGGTTGTGGTGCAAGTTCCTCTAGTCCGTAGGCAGGTTGCTCATTCCGTCGAGCTAGGCTCTTGACTCTGCTCCAAAGGCTA
TTGGACGAGGATCGACCGTGGCCCGGCGGACTTTGTCGATCCTTTGGTTTCTTTCTTTCTTGGTTCTTGGAGGGAAGGCGATATGAGGAATGCTGGAGCAAATCGGTTCTCGGGAGGGAAGTCGATATGACGAATCCTTACCCAAACGGAACAGGAAAGAAATCCGCTGGTTCATCTACCAGGAGTATGGCCCATTATTT
TTATAGGCTGTAGGGTTTATATACTGCACCATTGCATGAATTGTAAATTTCAGTTATTCTTGTAGGATTTATATACTTTCAATTTATAGTAACAAAAGATTTCCATCAAGACGATTCAGTGAATTAAATATACGAACATAAAAAATCTGGAAGGCTTGAACAATCATAAATATTGAAATAGGAATTCTTTTAAGTGGGAT
TTATCAAGATCGTTGAAATAGGGTGATCTTTGGCCGTCTTTGTTGAGATAAACGTAGTAGAGACTCTGCAGAAGATCCCCGAGGAACAACGCAAAGAAATCAGGAAAACCATAATCCAGGATGCTTTTATGATTTCCTTCATAAATGGAGATGGCAAACCATAATGGAGATGGAGGTACTTTCTGCCATCTCCTGTATAC
GCCTCACGATCTGCACAGTGCCACCGATAGAATTCAATAAAACAATTACAGCAAATACACAAAACATATATGAATAGCGTGAATAAGAAACAATTAAATTCAACATGTTTATGTTTAACTAATGATAAGTACCTCAATTAATGCTAATAAAGAACAGGTGAACATCGATCTAGTTTGCAGCTTCCTTAATGGGCCATCAC
ATTTCTTCTCGGGGCAGAGTTTTTTCTTTGAGGGAATTAATGATTTTTCTGATCGTCTTCGGGAACTCGAACTCTGAGTATCGGGCTGGAAATGACAGTTGTGAATAGATACTCAGAGTTCACTGCATTCTTGGCCATATTATATTATCAGCCATGGCATTCATTAGTCATATATTGATCGAGGAAAAAAATCTGTACAA
ATGAGTTCACTACATTCATTGGTCATATTATATTATCAGCCATGGCATTCATTAGTCATATATTGATCGAGGAAAAAAATCTGTACAAATGAGTTCACTACATTCATTGGTCATATTATATTATCAGCCATGGC

>TRINITY_DN122107_c0_g1_i8 from sample A1 / Example unspliced isoform:

CTCTCATTACCGTTAGATGTAGGTTTCTGAAAAAGCGAAAAACCTGCCTGTCCTTTTCGATGTCAGGAGGTGCGGAGCTATCGCTCACTCGTCTCTAACATCTCCACTACCTAACCTCACCACCTTCGATGATCGCGCAGTTACCTAACCACACCAGCTTCGATGATCGCGCAGTTAATGGATTATTGCTTCACTTGGAG
CTCATCCTGGATTATTCTAAACATTAACTTTACTGACTGCCATGGTGTTTTGGGCTCGAGATTAGGTTTTGGTATGCGATTTGGTCATTGTACATGGTTGACCTCTAGTTTAGTTCTGGTTTTGTTTCCGCACAGGGGCTTCAGTTTACTTGGATTATGGTTTCAATGTATGGTAGTTTGATGATGGCTCCTCTTTACTC
TTGCGAGGAACCCAATAACTGTCCATTCCTCTCTCCCCCAACTCAGTGGGTCGGTTGTGGTGCAAGTTTCTCTAGTCCGTAGGCATGTTCCTCATTCCGTCGAGCTAGGCTCTTGACTCTGCTCCAAAGgtaaaaggttatgtttatttctatttgcaaaggatttttcttatttaatggagttatttatcatcatactt
ttacattgtacattgtactgatcccggtattgatctccctagtagGATATTGGACGAGGATCGACCGTGGCCCGGCGGACTTTGTCGATCCTTTGGTTTCTTTCTTTCTTGGTTCTTGGAGGGAAGGCGATATGAGGAATGCGATATGAGGAATGCTGGAGCAAATCGGTTCTCTGGAGGGAAGGCGAAGGAACCCAATA
ACTGTCCATTCCTCTCTTCCCCAACTCAGTGAGTCGGTTGTGGTGCAAGTTCCTCTAGTCCGTAGGCAGGTTGCTCATTCCGTCGAGCTAGGCTCTTGACTCTGCTCCAAAGGCTATTGGACGAGGATCGACCGTGGCCCGGCGGACTTTGTCGATCCTTTGGTTTCTTTCTTTCTTGGTTCTTGGAGGGAAGGCGATAT
GAGGAATGCTGGAGCAAATCGGTTCTCGGGAGGGAAGTCGATATGACGAATCCTTACCCAAACGGAACAGGAAAGAAATCCGCTGGTTCATCTACCAGGAGTATGGCCCATTATTTTTATAGGCTGTAGGGTTTATATACTGCACCATTGCATGAATTGTAAATTTCAGTTATTCTTGTAGGATTTATATACTTTCAATT
TATAGTAACAAAAGATTTCCATCAAGACGATTCAGTGAATTAAATATACGAACATAAAAAATCTGGAAGGCTTGAACAATCATAAATATTGAAATAGGAATTCTTTTAAGTGGGATTTATCAAGATCGTTGAAATAGGGTGATCTTTGGCCGTCTTTGTTGAGATAAACGTAGTAGAGACTCTGCAGAAGATCCCCGAGG
AACAACGCAAAGAAATCAGGAAAACCATAATCCAGGATGCTTTTATGATTTCCTTCATAAATGGAGATGGCAAACCATAATGGAGATGGAGGTACTTTCTGCCATCTCCTGTATACGCCTCACGATCTGCACAGTGCCACCGATAGAATTCAATAAAACAATTACAGCAAATACACAAAACATATATGAATAGCGTGAAT
AAGAAACAATTAAATTCAACATGTTTATGTTTAACTAATGATAAGTACCTCAATTAATGCTAATAAAGAACAGGTGAACATCGATCTAGTTTGCAGCTTCCTTAATGGGCCATCACATTTCTTCTCGGGGCAGAGTTTTTTCTTTGAGGGAATTAATGATTTTTCTGATCGTCTTCGGGAACTCGAACTCTGAGTATCGG
GCTGGAAATGACAGTTGTGAATAGATACTCAGAGTTCACTGCATTCTTGGCCATATTATATTATCAGCCATGGCATTCATTAGTCATATATTGATCGAGGAAAAAAATCTGTACAAATGAGTTCACTACATTCATTGGTCATATTATATTATCAGCCATGGCATTCATTAGTCATATATTGATCGAGGAAAAAAATCTGT
ACAAATGAGTTCACTACATTCATTGGTCATATTATATTATCAGCCATGGC