sp|Q8GXZ3|PBL8_ARATH ----> Probable serine/threonine-protein kinase PBL8

Intron 1 GTATGCACGC ... TTGTTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.11	17.8	7.8	33.3
A2	72.99	11	3.8	32
A3	76.05	9.8	6	31.5
A4	76.54	13.8	16	48.5
A5	95.69	1	10	22.5
A6	78.34	12	18.8	45
A7	80.2	25	15	104.5
A8	76.69	10	10	33.8
A9	78.66	20.3	33.3	76
A10	74.71	26.5	14	82.8

>TRINITY_DN116140_c0_g2_i2 from sample A1 / Example spliced isoform

GAAAAAACAAAGTCCAAGACGACCATGGCAAAATGAATTATACACAGCACAAACCGTGTGTATATCTCTTTATCTCCCACAACCATTTAGAACACAATATTTTCGTCAAACACGATTTCCAATTGTGGCTATGCTCTCTCGTATTTATACTAAATTAATACACGATCTCAGCGCTCGTTAGGTAGGCGGGTTCATATGTC
AAGGGATTAATGGGAAACTGCTGGACCGGAGAGCAGGGCTCTTTGAGCAGAGTCTCGTCTCATAACAAATCATCCTACCCGCCATCCCCATCAACAACACACGGGCCAATTCCAACAGACAGCAATGGAGGAAATTCAGTTACTCCGTTATCAGCCACATATGAGGTCGAAGACCTGAGACAGAACTCTGATTCCAATGT
TTATGTTTTCACATTTAATGAGCTCAAGGCAATTACCAAAAACTTCAGGGTGGATAATGTATTGGGAGAAGGAGGGTTCGGTACAGTTTACAAGGGTAACATTGATGAGGATATGAAGCCAGGCCTCGATGCCCAGCTAGTTGCTGTCAAGGTCCTTGATCCGGAAGGTCACCAGGGGCACAGAGAATGGCTGGCAGAGG
TCATCTTTCTAGGGCAGCTGAGTCATCCCCATCTGGTGAAACTCATCGGATACTGTTGCGAAGCTGAGCACAGGTTGCTGGTTTACGAGTATATGGCTCGTGGAAGTCTGGAAAATCATCTATTCCGAAAGATGTCTGTTCCTCTGCCTTGGTCAACAAGAATGAAGATTGCACTCGGGGCTGCCGAAGGGCTTGCTTTT
CTTCATGGGGCAGAAAGGCCTGTTATCTATCGCGATTTCAAGACGTCCAATATACTACTAGACACAGACTATACAGCAAAGCTTTCAGACTTTGGGCTTGCAAAAGGGGGACCAGAAGGTGACAAGACTCATGTTTCCACTCGCGTGATGGGTACATATGGTTATGCCGCTCCAGAGTACGTCATGACTGGTCATTTGAC
AGCTCGAAGTGATGTCTACAGCTTCGGTGTTGTATTGCTTGAGCTGCTTACTGGCAGGCGGTCTATGGATAAGACTAGGCCTAGCAGAGAATATAACTTGGCTGAGTGGGCTCGTCCACTTCTCAATGAGAAGAGGAAGTTGTTACGGATACTTGATCCTAGAATGGAGGGTGAGTATTCTGTTAAGGGAGCTCTGAAAG
CTGCTAACTTGGCCTATCACTGCCTCAGCCAAAATCCTAAAGCAAGGCCGCTTATGAAGGATGTTGTCGAGACGTTGGAACCTCTGCAAATTATGACAGATATGGAAAATACTTCCTTCACATATTCTGTTGGTAGTGGTGTGACTACATATGAGGTTCGCTCTCAGTCAACTGAAAATGGTATCTAAGAAAATGACTGC
AAGCATCATCAGATAACTTACTGTTATAAATGAAGTTGCAATGTTGCAAGATTACTCCTGGTTCATATAAACAATCTTGGAAGCATCCATATAGAAGTGTAGATGTGCAGAGTTTCCCATTCCTCTGAAGAGGATCTGTCTCTTCCTGTTGTACGAGGTAAACTACTTACAGTCTCTACTCCGCATCCACTCATCAATCT
GCTGAAGATCCCCTTTAACCTTCATAGATGATAATGTTATTTCTTAATCACCTCTGGTAAGGAATGGCTTATTGTACTCTGAATAAAGGGAAAATACTAATCTTTGCTGACTCAGAAGGTAAGGTACATGATACTTGTAAGCTGCAAGTTCGCACATTGTTTCTTAGATCACTGTGAGGCCTTAGTCAGTCATATTTTTT
GTTTCAGAAAGTGGTAGATTATTTGATCCTTCATCTGCTACCTGTATATAAATTTGTATTTGCTACTGCTAGAAAAGTACGAATAGCTGTGTATTATCTACAGAGTATAATTTTCCATTAAATGAACCTTGGTGGAGCCTTCATATTCATTTCTACAAAGACAAGTTGTTATGAAAAAACTTGTCTGGGACTGCCCATAA
AGGGCCACGTTTCTGTATTTGAGCTAAAACATTTTTACTGTTCATAAGCAAAA

>TRINITY_DN116140_c0_g2_i3 from sample A1 / Example unspliced isoform:

GAAAAAACAAAGTCCAAGACGACCATGGCAAAATGAATTATACACAGCACAAACCGTGTGTATATCTCTTTATCTCCCACAACCATTTAGAACACAATATTTTCGTCAAACACGATTTCCAATTGTGGCTATGCTCTCTCGTATTTATACTAAATTAATACACGATCTCAGCGCTCGTTAGGTAGGCGGGTTCATATGTC
AAGgtatgcacgcaaatgaaaagagggaataagaatctagtctttccctattctccgcctgaagtgtattgtgaatgggctgacttttgatcgccattaaaggcaggctttgatttcaaggcctgatcaccaatattgaggcgatgtacagggtagtttgattgaaggaaaaataaaaactatgcattgttggcagGGAT
TAATGGGAAACTGCTGGACCGGAGAGCAGGGCTCTTTGAGCAGAGTCTCGTCTCATAACAAATCATGTAAGTTTGGTCTAGCCTGCCTCATCAATATCAATGTAAATTCTTCAGTTTTATTACTTCTTTCTTCATCTGCATCGTTACAATTATGTTCAGACAGGTGACTATAGATCTGCACCATGCTTTTTAG

Intron 2 GTAAGTATAA ... ATTGTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	33.33	2	2	0
A3	100	0	1	1
A4	-1	0	0	0
A5	-1	0	0	0
A6	71.43	2	9	1
A7	100	0	5	2
A8	100	0	9	13
A9	66.67	3	9	3
A10	80	1	7	1

>TRINITY_DN39848_c0_g1_i7 from sample A9 / Example spliced isoform

CTGGAAAATCATCTATTCCGAAACTCTAAGAGAGAGAGAGTGCCGCGTGGAAGTGATTTCTTGACGTTCTAGTGCATGGTGGTGATCCAGCTCGGGGGTGGAGTTGGGAAAACAGTCGAGTTGCTCTCTCCTATGGTTGGGAGGAGCTGACTCTCCAAGTAGCCATTTGTTTACATTTCAATTCAATGAATATTTGACGA
ATTTACATACATGGGCTCGGGTTCTCTCTCGACTATGGGTG

>TRINITY_DN39848_c0_g1_i6 from sample A9 / Example unspliced isoform:

ATTAAAAATGGCCATTATCTAGATCACTGTAGAATAGAAAAAACAAAGTCCAAGACGACCATGGCAAAATGAATTATACACAGCACAAACCGTGTGTATATCTCTTTATCTCCCACAACCATTTAGAACACAATATTTTCGTCAAACACGATTTCCAATTGTGGCTATGCTCTCTCGTATTTATACTAAATTAATACACG
ATCTCAACGCTCGTTAGGTAAGTGGGTTCATATGTCGAGGTATGCACGCAAATGAAAAGAGGGAATAAGAATCTAGTCTTTCCCTATTCTCCGCCTGAAGTGTATTGTGAATGGGCTGACTTTTGATCGCCATTAAAGGCAGGCTTTGATTTCAAGGCCTGATCACCAATATTGAGGCGATGTACAGGGTAGTTTGATTG
AAGGAAAAATAAAAACTATGCATTGTTGGCAGGGATTAATGGGAAACTGCTGGACCGGAGAGCAGGGCTCTTTGAGCAGAGTCTCGTCTCATAACAAATCATCCTACCCGCCATCCCCATCAACAACACACGGGCCAATTCCAACAGACAGCAATGGAGGAAATTCAGTTACTCCGTTATCAGCCACATATGAGGTCGAA
GACCTGAGACAGAACTCTGATTCCAATGTTTATGTTTTCACATTTAATGAGCTCAAGGCAATTACCAAAAACTTCAGGGTGGATAATGTATTGGGAGAAGGAGGGTTCGGTACAGTTTACAAGGGTAACATTGATGAGGATATGAAGCCAGGCCTCGATGCCCAGCTAGTTGCTGTCAAGGTCCTTGATCCGGAAGGTCA
CCAGGGGCACAGAGAATGGCTGGCAGAGGTCATCTTTCTAGGGCAGCTGAGTCATCCCCATCTGGTGAAACTCATCGGATACTGTTGCGAAGCTGAGCACAGGTTGCTGGTTTACGAGTATATGGCTCGTGGAAGTCTGGAAAATCATCTATTCCGAAAGATGTCCGTTCCTCTGCCTTGGTCAACAAGAATGAAGATTG
CACTCGGGGCTGCCGAAGGGCTTGCTTTTCTTCATGGGGCAGAAAGGCCTGTTATCTATCGCGATTTCAAGACGTCCAATATACTACTAGACACAGACTATACAGCAAAGCTTTCAGATTTTGGGCTTGCAAAAGGGGGACCAGAAGGTGACAAGACTCATGTTTCCACTCGCGTGATGGGTACATATGGTTATGCCGCT
CCAGAGTACGTCATGACTGGTCATTTGACAGCTCGAAGTGATGTCTACAGCTTCGGTGTTGTATTGCTTGAGCTGCTTACTGGCAGGCGGTCTATGGATAAGACTAGGCCTAGCAGAGAATATAATTTGGCTGAGTGGGCTCGTCCACTTCTCAATGAGAAGAGGAAGTTGTTACGGATACTTGATCCTAGAATGGAGGG
TGAGTATTCTGTTAAGGGAGCTCTGAAAGCTGCTAACTTGGCCTATCACTGCCTCAGCCAAAATCCTAAAGCAAGGCCGCTTATGAAGGATGTTGTCGAGACGTTGGAACCTCTGCAAATTATGACAGATATGGAAAATACTTCCTTCACATATTCTGTTGGTAGTGGTGTGACTACATATGAGGTTCGCTCTCAGTCAA
CTGAAAATGGTATCTAAGAAAATGACTGCAAGCATCATCAGATAACTTACTGTTATAAATGAAGTTGCAATGTTGCAAGATTACTCCTGGTTCATATAAACAATCTTGGAAGCATCCATATAGAAGTGTAGATGTGCAGAGTTTCCCATTCCTCTGAAGAGGATCTGTCTCTTCCTGTTGTACGAGACTCTAAGAGAGAG
AGAGTGCCGCGTGGAAGTGATTTCTTGACGTTCTAGTGCATGGTGGTGATCCAGCTCGGGGGTGGAGTTGGGAAAACAGTCGAGTTGCTCTCTCCTATGGTTGGGAGGAGCTGACTCTCCAAGTAGCCATTTGTTTACATTTCAAgtaagtataactctgttcttgtataaatagtcatcgaatgtatgaaaaaatacgc
tgacttttatttttatcagcttatatacctgttgtacaatctattgtaaatcatggattattgattgtatcagTTCAATGAATATTTGACGAATTTACATACATGGGCTCGGGTTCTCTCTCGACTATGGGTG