sp|Q9FG14|MYOB7_ARATH ----> Myosin-binding protein 7


Intron 1 GTAGGTGCAA ... GACCTGGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A147.13232219
A247.92252818
A353.04272833
A444.53383724
A555.84172518
A633.33550
A750.77161716
A875124
A951.61151616
A1038.98362323



>TRINITY_DN125058_c0_g1_i8 from sample A1 / Example spliced isoform
GTTGGAAGGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAA
AGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGAGTCTTTTACAAAGTCTATAAGAAATCGACTTCAATTCTGAATCTCACTGGTTTTTTGCGGGCGTCATTCGCTCGCATGCCTGAGTCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGA
GAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCA
GGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTC
TCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGA
AAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTG
TCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGACGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGG
TTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAA
AAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATG
AATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAA
TGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTG
GAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTA
TACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGT
ATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAG
CTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCA
ACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGA
CTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCAT
TAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACC


>TRINITY_DN125058_c0_g1_i6 from sample A1 / Example unspliced isoform:
GTTGGAAGGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAA
AGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGgtaggtgcaagtgcaaatgatccggtaaacgcgtaaagggaaataaaggatgaagaataagggcattacggcttttcgatttgtttaaactacggatttgaatgaatctgcatatttacacctgt
agtaagtggttttgtgacctggcagAGTCTTTTACAAAGTCTATAAGAAATCGACTTCAATTCTGAATCTCACTGGTTTTTTGCGGGCGTCATTCGCTCGCATGCCTGAGTCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTG
AGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGA
GGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAA
GTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAA
ACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGC
CAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGACGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATC
CAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACA
TGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGC
AAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGC
AGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGAT
CTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAA
AGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAG
AGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTC
AAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTT
CTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTT
AATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATAC
TATTTTGGATAGACTTACC



Intron 2 GTATGCTACT ... TATCCCAAAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A135.5732.61810.5
A233.3733.9175
A335.2647.82613.6
A432.5960.929.51.9
A528.0741163
A643.75976
A744.8121.917.84.9
A816.879.921.9
A926.5333.912.22.9
A1026.0156.9206.8



>TRINITY_DN113498_c0_g1_i6 from sample A2 / Example spliced isoform
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGAAATGAAAGGAAGATTTCTCTTTCGATTTCTTTATTAGGTAGGTGCAAGTGCAAATGATCCGGTAAACGCGTAAAGGGAAATAAAGGATGAAGAATAAGGGCATTACGGCTTTTCGATTTGTTTAAACTACGGATTTGAATGAATCTGCATATTTACACCT
GTAGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATG
AGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAA
TTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTC
TTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGG
AGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGT
GAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACA
TTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATA
GGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGT
GAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGG
AGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCA
ATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAA
AGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAA
AAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCA
ACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTT
ATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTT
GTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA


>TRINITY_DN113498_c0_g1_i12 from sample A2 / Example unspliced isoform:
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGgtatgctacttactctactctacaatgtggacctgaaattgaaattgttgctttcataatgcctttccaacgtcactggttggaatcccgacctgcaaagatagttgataaaccggcctttcatatcccaaagGAAATGAAAGGAAGATTTCTCTTTCGATT
TCTTTATTAGGTAGGTGCAAGTGCAAATGATCCGGTAAACGCGTAAAGGGAAATAAAGGATGAAGAATAAGGGCATTACGGCTTTTCGATTTGTTTAAACTACGGATTTGAATGAATCTGCATATTTACACCTGTAGTAAGTGGTTTTGTGACCTGGCAGAGTCTTTTACAAAGTCTATAAGAAATCGACTTCAATTCTG
AATCTCACTGGTTTTTTGCGGGCGTCATTCGCTCGCATGCCTGAGTCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGC
TCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACAT
GGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACT
GAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCG
TAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTG
AAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTT
GATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTC
TAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAG
AACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGG
GAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGA
AGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGC
AGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCA
CTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAA
CATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGT
ACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTA
TTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA



Intron 3 GCTCAGGCTA ... TTTTGTGAAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A135.56582836
A231.82452022
A334.63673536
A429.76723328
A529.79331711
A625951
A720.8836109
A80600
A927.78391317
A1030.16442216



>TRINITY_DN113498_c0_g1_i7 from sample A2 / Example spliced isoform
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAA
TTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATG
ACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCG
AAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAAC
ATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTA
TTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGAC
AAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAA
AATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTA
GCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAAC
ACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGA
TGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCC
AGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGAT
ATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGAC
ACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTC
ATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCG
TCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA


>TRINITY_DN113498_c0_g1_i8 from sample A2 / Example unspliced isoform:
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGgctcaggctaggtcgtgggtttgaattttataggagaggagtcccagaattgttgtagcatgagacgaggtaatacattcagtgcgtattttgtactaaggttttaatatgtacatttccagcgttttgttttgtgctttatgttagcaatatctgtaaagg
tttgacaccattttgaggcagtcgagtggaagctgcatttactttggcatttacatttattcaaagaatcgttgtttgttagtggcttatgttttttttcttgttgcaatgtatacttttttgcttcattttctgggagattggaatcctttctctgattaacgaggcattgttttttgtgaagGACATAACGCAGCTTG
AGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGA
GGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAA
GTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAA
ACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGC
CAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATC
CAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACA
TGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGC
AAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGC
AGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGAT
CTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAA
AGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAG
AGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTC
AAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTT
CTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTT
AATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATAC
TATTTTGGATAGACTTACTCTCTAAA



Intron 4 GTATGCTACT ... GACCTGGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A144.58231819
A241.18251718
A352.21272633
A441.09382924
A550171618
A641.18570
A751.52161816
A875124
A948.28151216
A1037.39362023



>TRINITY_DN112061_c0_g1_i4 from sample A3 / Example spliced isoform
GGCTCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGAGTCTTT
TACAAAGTCTATAAGAAATCGACTTCAATTCTGAATCTCACTGGTTTTTTGCGGGCGTCATTCGCTCGCATGCCTGAGTCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTG
CATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTG
ATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACT
CACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGA
AAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAAC
TTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGG
ACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGG
TCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAA
CACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGT
CATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAA
GCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATG
AGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGG
CAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGT
TTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAG
CAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTA
TATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAGTATTGAG
TGTTCAATATAAATGCAATAAAATGTCTGAAATGCTT


>TRINITY_DN112061_c0_g1_i5 from sample A3 / Example unspliced isoform:
GGCTCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGgtatgct
acttactctactctacaatgtggacctgaaattgaaattgttgctttcataatgcctttccaacgtcactggttggaatcccgacctgcaaagatagttgataaaccggcctttcatatcccaaaggaaatgaaaggaagatttctctttcgatttctttattaggtaggtgcaagtgcaaatgatccggtaaacgcgta
aagggaaataaaggatgaagaataagggcattacggcttttcgatttgtttaaactacggatttgaatgaatctgcatatttacacctgtagtaagtggttttgtgacctggcagAGTCTTTTACAAAGTCTATAAGAAATCGACTTCAATTCTGAATCTCACTGGTTTTTTGCGGGCGTCATTCGCTCGCATGCCTGAG
TCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAA
TGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATC
AATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGT
TCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGA
GGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGT
GTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAA
CATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCA
TAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCT
GTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAA
GGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGC
CAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTG
AAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTA
AAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGCTATGTTAAGTTTTTCCATATAGTGTTTTGCAACATTCAAAAATTGTCAGTTCATGCCTGG
GGTTTTGGAGCAGTTATTTTTCCTGCAGGCATCTTGTAGGGTAAGGAATCTGTATCAGCTGCACAGTACAAAGGGTACTATCATGTGGGTTGCTGGTAGCATGTTAACTTCAACCTATCTACACCATTGAACATGGGCCGTACACAACCCATCATATGAAACCATCTCAAGCATGTGCTGGCACATAAGTCTTTAAAAGT
GGGTGGTCAATATTCAACTCTTCTCATGTTTGTGAGATCGGAGTGGATTGAGGAGAGACTATAGAAATGTTTATGTCGAATTATGGTATATTTACTTCCAGCTTGTATTATCTTATATTGTAAACAGCACCAAGAAACGTGGCAGATACATGGACAGGAGCAGATGGGGAATGGGAAGTGAGGAGGATTTG



Intron 5 GTATGCTACT ... ATGAGACGAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A140.21581860
A240451743
A338.81672659
A435.537229.450
A538.89331626
A640975
A728.583617.811
A820621
A932.283912.225
A1038.89442036



>TRINITY_DN113498_c0_g1_i7 from sample A2 / Example spliced isoform
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAA
TTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATG
ACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCG
AAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAAC
ATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTA
TTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGAC
AAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAA
AATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTA
GCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAAC
ACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGA
TGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCC
AGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGAT
ATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGAC
ACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTC
ATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCG
TCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA


>TRINITY_DN113498_c0_g1_i12 from sample A2 / Example unspliced isoform:
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGgtatgctacttactctactctacaatgtggacctgaaattgaaattgttgctttcataatgcctttccaacgtcactggttggaatcccgacctgcaaagatagttgataaaccggcctttcatatcccaaaggaaatgaaaggaagatttctctttcgatt
tctttattaggtaggtgcaagtgcaaatgatccggtaaacgcgtaaagggaaataaaggatgaagaataagggcattacggcttttcgatttgtttaaactacggatttgaatgaatctgcatatttacacctgtagtaagtggttttgtgacctggcagagtcttttacaaagtctataagaaatcgacttcaattctg
aatctcactggttttttgcgggcgtcattcgctcgcatgcctgagtccaggctcaggctaggtcgtgggtttgaattttataggagaggagtcccagaattgttgtagcatgagacgagGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGC
TCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACAT
GGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACT
GAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCG
TAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTG
AAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTT
GATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTC
TAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAG
AACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGG
GAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGA
AGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGC
AGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCA
CTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAA
CATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGT
ACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTA
TTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA



Intron 6 GTAGGTGCAA ... ATGAGACGAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A141.41582260
A244.1452843
A339.37672859
A437.66723750
A543.59332526
A635.71955
A728361711
A820621
A934.45391625
A1040.14442336



>TRINITY_DN125058_c0_g1_i5 from sample A1 / Example spliced isoform
GTTGGAAGGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAA
AGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGA
ATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAAT
CAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCG
TTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGG
AGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGACGAGGAAATTGCTTCCCTCGAG
TGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGA
ACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTC
ATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTC
TGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGA
AGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTG
CCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACT
GAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCT
AAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGCTATGTTAAGTTTTTCCATATAGTGTTTTGCAACATTCAAAAATTGTCAGTTCATGCCTG
GGGTTTTGGAGCAGTTATTTTTCCTGCAGGCATCTTGTAGGGTAAGGAATCTGTATCAGCTGCACAGTACAAAGGGTACTATCATGTGGGTTGCTGGTAGCATGTTAACTTCAACCTATCTACACCATTGAACATGGGCCGTACACAACCCATCATATGAAACCATCTCAAGCATGTGCTGGCACATAAGTCTTTAAAAG
TGGGTGGTCAATATTCAACTCTTCTCATGTTTGTGAGATCGGAGTGGATTGAGGAGAGACTATAGAAATGTTTATGTCGAATTATGGTATATTTACTTCCAGCTTGTATTATCTTATATTGTAAACAGCACCAAGAAACGTGGCAGATACATGGACAGGAGCAGATGGGG


>TRINITY_DN125058_c0_g1_i6 from sample A1 / Example unspliced isoform:
GTTGGAAGGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAA
AGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGgtaggtgcaagtgcaaatgatccggtaaacgcgtaaagggaaataaaggatgaagaataagggcattacggcttttcgatttgtttaaactacggatttgaatgaatctgcatatttacacctgt
agtaagtggttttgtgacctggcagagtcttttacaaagtctataagaaatcgacttcaattctgaatctcactggttttttgcgggcgtcattcgctcgcatgcctgagtccaggctcaggctaggtcgtgggtttgaattttataggagaggagtcccagaattgttgtagcatgagacgagGACATAACGCAGCTTG
AGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGA
GGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAA
GTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAA
ACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGC
CAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGACGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATC
CAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACA
TGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGC
AAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGC
AGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGAT
CTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAA
AGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAG
AGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTC
AAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTT
CTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTT
AATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATAC
TATTTTGGATAGACTTACC



Intron 7 GCGTCATTCG ... CTGAGTCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A136.8412410
A226.0327109
A335.5919147
A4301484
A548.15794
A660112
A747.83692
A8100011
A920831
A1011.111212



>TRINITY_DN113498_c0_g1_i6 from sample A2 / Example spliced isoform
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGAAATGAAAGGAAGATTTCTCTTTCGATTTCTTTATTAGGTAGGTGCAAGTGCAAATGATCCGGTAAACGCGTAAAGGGAAATAAAGGATGAAGAATAAGGGCATTACGGCTTTTCGATTTGTTTAAACTACGGATTTGAATGAATCTGCATATTTACACCT
GTAGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATG
AGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAA
TTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTC
TTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGG
AGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGT
GAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACA
TTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATA
GGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGT
GAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGG
AGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCA
ATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAA
AGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAA
AAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCA
ACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTT
ATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTT
GTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA


>TRINITY_DN113498_c0_g1_i11 from sample A2 / Example unspliced isoform:
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGAAATGAAAGGAAGATTTCTCTTTCGATTTCTTTATTAGGTAGGTGCAAGTGCAAATGATCCGGTAAACGCGTAAAGGGAAATAAAGGATGAAGAATAAGGGCATTACGGCTTTTCGATTTGTTTAAACTACGGATTTGAATGAATCTGCATATTTACACCT
GTAgcgtcattcgctcgcatgcctgagtccagGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTA
AATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATG
TTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTC
AAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTT
ACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGA
AAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTT
GGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGG
ACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAA
GTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTA
GTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAA
TAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATA
GTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAG
TTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATAT
GCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCT
GCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAA
ATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA



Intron 8 GTAGGTGCAA ... CTGAGTCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A138.74342221
A253.57262832
A337.98402821
A445.74353722
A551.35182513
A636.84652
A756.5210179
A8100021
A950141612
A1050242325



>TRINITY_DN125058_c0_g1_i2 from sample A1 / Example spliced isoform
GTTGGAAGGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAA
AGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAA
GCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAAT
CTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAG
AACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAG
TTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCAT
GATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGACGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTC
CTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCAC
AACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGAT
TATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGA
TGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGG
CAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGA
CTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGA
ACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATT
TCATGAAGGTATACTGTAGTCTGCTTGTATTAGTTGATGTTCCTGTTGTATATTTTGCTTGATGTTTGGCATGCATTTTCCTGTTAAATCAAATAATTTGTCTTCAAATGTGTCTGTGTATATTTGTGTAATAGTCTATAGTTTCTGAACTTCTTGGTAGCAGTCTATTTTTAATATTTATTTATATAAATTTTGATTAG
CAAGTTTTTGGAGATTTTAAAATGTATGTTCATTTTCTATTCAAATGTACAATGCAAAGCAACAATCTGGGTGGGGCAATGTTGTCAGTGCTGTTTGATCTAGC


>TRINITY_DN125058_c0_g1_i6 from sample A1 / Example unspliced isoform:
GTTGGAAGGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAA
AGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGgtaggtgcaagtgcaaatgatccggtaaacgcgtaaagggaaataaaggatgaagaataagggcattacggcttttcgatttgtttaaactacggatttgaatgaatctgcatatttacacctgt
agtaagtggttttgtgacctggcagagtcttttacaaagtctataagaaatcgacttcaattctgaatctcactggttttttgcgggcgtcattcgctcgcatgcctgagtccagGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTG
AGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGA
GGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAA
GTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAA
ACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGC
CAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGACGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATC
CAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACA
TGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGC
AAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGC
AGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGAT
CTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAA
AGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAG
AGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTC
AAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTT
CTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTT
AATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATAC
TATTTTGGATAGACTTACC



Intron 9 GTAAGTGGTT ... CTGAGTCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A163.89.91320.5
A249.6125.11731.5
A363.7217.64120.6
A462.1413.12121.8
A571.936.82212.9
A658.330.801.9
A768.596179
A8100040.9
A964.627.71611.9
A1062.1111.11124.8



>TRINITY_DN125058_c0_g1_i13 from sample A1 / Example spliced isoform
GGTTGGAATCCCGACCTGCAAAGATAGTTGATAAACCGGCCTTTCATATCCCAAAGGAAATGAAAGGAAGATTTCTCTTTCGATTTCTTTATTAGGTAGGTGCAAGTGCAAATGATCCGGTAAACGCGTAAAGGGAAATAAAGGATGAAGAATAAGGGCATTACGGCTTTTCGATTTGTTTAAACTACGGATTTGAATGA
ATCTGCATATTTACACCTGTAGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGG


>TRINITY_DN125058_c0_g1_i6 from sample A1 / Example unspliced isoform:
GTTGGAAGGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAA
AGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGTAGGTGCAAGTGCAAATGATCCGGTAAACGCGTAAAGGGAAATAAAGGATGAAGAATAAGGGCATTACGGCTTTTCGATTTGTTTAAACTACGGATTTGAATGAATCTGCATATTTACACCTGT
AgtaagtggttttgtgacctggcagagtcttttacaaagtctataagaaatcgacttcaattctgaatctcactggttttttgcgggcgtcattcgctcgcatgcctgagtccagGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTG
AGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGA
GGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAA
GTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAA
ACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGC
CAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGACGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATC
CAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACA
TGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGC
AAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGC
AGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGAT
CTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAA
AGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAG
AGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTC
AAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTT
CTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTT
AATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATAC
TATTTTGGATAGACTTACC



Intron 10 GTATGCTACT ... CTGAGTCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A136.45341821
A248.51261732
A337.01402621
A442.273529.322
A544.62181613
A642.86672
A757.331017.99
A8100021
A946.281412.112
A1048.39242025



>TRINITY_DN113498_c0_g1_i10 from sample A2 / Example spliced isoform
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCT
GTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAG
AGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAA
GAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCA
GGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATAC
ATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGAT
GCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTAC
ACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTA
GTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAA
ACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTC
AACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAG
AAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGAC
AGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAG
TAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAA
GGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAAT
TTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA


>TRINITY_DN113498_c0_g1_i12 from sample A2 / Example unspliced isoform:
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGgtatgctacttactctactctacaatgtggacctgaaattgaaattgttgctttcataatgcctttccaacgtcactggttggaatcccgacctgcaaagatagttgataaaccggcctttcatatcccaaaggaaatgaaaggaagatttctctttcgatt
tctttattaggtaggtgcaagtgcaaatgatccggtaaacgcgtaaagggaaataaaggatgaagaataagggcattacggcttttcgatttgtttaaactacggatttgaatgaatctgcatatttacacctgtagtaagtggttttgtgacctggcagagtcttttacaaagtctataagaaatcgacttcaattctg
aatctcactggttttttgcgggcgtcattcgctcgcatgcctgagtccagGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGC
TCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACAT
GGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACT
GAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCG
TAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTG
AAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTT
GATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTC
TAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAG
AACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGG
GAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGA
AGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGC
AGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCA
CTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAA
CATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGT
ACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTA
TTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA



Intron 11 GTAATACATT ... TTTTGTGAAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A137.560.1836
A236.7738.21022
A338.4157.89.736
A437.8946.17.728
A533.2822.4411
A618.154.60.31
A745.7910.70.79
A800.600
A940.8124.70.717
A1032.2133.83.316



>TRINITY_DN125058_c0_g1_i2 from sample A1 / Example spliced isoform
GTTGGAAGGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAA
AGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAA
GCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAAT
CTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAG
AACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAG
TTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCAT
GATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGACGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTC
CTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCAC
AACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGAT
TATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGA
TGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGG
CAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGA
CTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGA
ACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATT
TCATGAAGGTATACTGTAGTCTGCTTGTATTAGTTGATGTTCCTGTTGTATATTTTGCTTGATGTTTGGCATGCATTTTCCTGTTAAATCAAATAATTTGTCTTCAAATGTGTCTGTGTATATTTGTGTAATAGTCTATAGTTTCTGAACTTCTTGGTAGCAGTCTATTTTTAATATTTATTTATATAAATTTTGATTAG
CAAGTTTTTGGAGATTTTAAAATGTATGTTCATTTTCTATTCAAATGTACAATGCAAAGCAACAATCTGGGTGGGGCAATGTTGTCAGTGCTGTTTGATCTAGC


>TRINITY_DN125058_c0_g1_i9 from sample A1 / Example unspliced isoform:
GTTGGAAGGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAA
AGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGAAATGAAAGGAAGATTTCTCTTTCGATTTCTTTATTAGGTAGGTGCAAGTGCAAATGATCCGGTAAACGCGTAAAGGGAAATAAAGGATGAAGAATAAGGGCATTACGGCTTTTCGATTTGTTT
AAACTACGGATTTGAATGAATCTGCATATTTACACCTGTAGTAAGTGGTTTTGTGACCTGGCAGAGTCTTTTACAAAGTCTATAAGAAATCGACTTCAATTCTGAATCTCACTGGTTTTTTGCGGGCGTCATTCGCTCGCATGCCTGAGTCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCA
GAATTGTTGTAGCATGAGACGAGgtaatacattcagtgcgtattttgtactaaggttttaatatgtacatttccagcgttttgttttgtgctttatgttagcaatatctgtaaaggtttgacaccattttgaggcagtcgagtggaagctgcatttactttggcatttacatttattcaaagaatcgttgtttgttagtg
gcttatgttttttttcttgttgcaatgtatacttttttgcttcattttctgggagattggaatcctttctctgattaacgaggcattgttttttgtgaagGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAA
ATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGT
TGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCA
AGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTA
CGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAA
AGACGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTG
GTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGA
CAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAG
TCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAG
TAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAAT
AAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAG
TTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGT
TTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATG
CACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTG
CATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAA
TATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACC



Intron 12 GTAATACATT ... CATAACGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A191.494543
A263.8913723
A385.716636
A472.9710527
A563.648114
A666.67102
A793.331114
A8-1000
A91000120
A1079.316323



>TRINITY_DN112061_c0_g1_i2 from sample A3 / Example spliced isoform
GGCTCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGAAATGA
AAGGAAGATTTCTCTTTCGATTTCTTTATTAGGTAGGTGCAAGTGCAAATGATCCGGTAAACGCGTAAAGGGAAATAAAGGATGAAGAATAAGGGCATTACGGCTTTTCGATTTGTTTAAACTACGGATTTGAATGAATCTGCATATTTACACCTGTAGTAAGTGGTTTTGTGACCTGGCAGAGTCTTTTACAAAGTCTA
TAAGAAATCGACTTCAATTCTGAATCTCACTGGTTTTTTGCGGGCGTCATTCGCTCGCATGCCTGAGTCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGCTTGAGAACTTCTGCAAAAGT


>TRINITY_DN112061_c0_g1_i13 from sample A3 / Example unspliced isoform:
TATGCTACTTACTCTACTCTACAATGTGGACCTGAAATTGAAATTGTTGCTTTCATAATGCCTTTCCAACGTCACTGGTTGGAATCCCGACCTGCAAAGATAGTTGATAAACCGGCCTTTCATATCCCAAAGGAAATGAAAGGAAGATTTCTCTTTCGATTTCTTTATTAGGTAGGTGCAAGTGCAAATGATCCGGTAAA
CGCGTAAAGGGAAATAAAGGATGAAGAATAAGGGCATTACGGCTTTTCGATTTGTTTAAACTACGGATTTGAATGAATCTGCATATTTACACCTGTAGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGgtaatacattcagtgcgtattttgtactaaggtt
ttaatatgtacatttccagcgttttgttttgtgctttatgttagcaatatctgtaaaggtttgacaccattttgaggcagtcgagtggaagctgcatttactttggcatttacatttattcaaagaatcgttgtttgttagtggcttatgttttttttcttgttgcaatgtatacttttttgcttcatttcctgggagat
tggaatcctttctctgattaacgaggcattgttttttgtgaaggacataacgcagCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAAT
TGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAG
CAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTT
CCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAAT
GAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCT
GAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGAC
AAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCC
AGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGC
CCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAG
ATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTG
CATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAA
TGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCA
AGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAG
AAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGC
AAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATT
CTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAGTATTGAGTGTTCAATATAAATGCAATAAAATGTCTGAAATGCTT



Intron 13 GTAGGTGCAA ... ATGAGACGAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A140.21582256
A240.4452833
A337.67672853
A435.71723743
A539.45332518
A633.33954
A728361711
A814.29620
A934.45391625
A1038.89442333



>TRINITY_DN113498_c0_g1_i7 from sample A2 / Example spliced isoform
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAA
TTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATG
ACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCG
AAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAAC
ATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTA
TTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGAC
AAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAA
AATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTA
GCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAAC
ACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGA
TGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCC
AGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGAT
ATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGAC
ACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTC
ATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCG
TCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA


>TRINITY_DN113498_c0_g1_i3 from sample A2 / Example unspliced isoform:
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGgtaggtgcaagtgcaaatgatccggtaaacgcgtaaagggaaataaaggatgaagaataagggcattacggcttttcgatttgtttaaactacggatttgaatgaatctgcatatttacacctgtagctcaggctaggtcgtgggtttgaattttataggag
aggagtcccagaattgttgtagcatgagacgagGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAG
GCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCT
CCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAA
AATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGT
CGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGT
TTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAA
AGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGA
ATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAAT
GACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGG
AATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTAT
ACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTA
TCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGC
TCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAA
CAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGAC
TTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATT
AGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA



Intron 14 GTATGTATTG ... GCTACTTCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A110.24405
A226.2345316
A3184109
A411.763014
A58.331101
A622.63106031
A721.66123134
A816.1172033
A916.56136027
A1016.6785017



>TRINITY_DN42612_c1_g1_i3 from sample A7 / Example spliced isoform
GGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGC
GTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGA
TCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTT
CAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCAT
GGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCAC
TTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGG
AATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACA
GAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCA
GCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATA
TCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAA
TGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGT
ATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAA
AACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTT
AGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAA
GGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCA
TGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAG
GCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAGTATTGAGTGTTCAATATAAATG
CAATAAAATGTCTGAAATGCTTCGGTTGCATTTATTC


>TRINITY_DN42612_c1_g1_i8 from sample A7 / Example unspliced isoform:
GTAATATGCACAAgtatgtattgctttgaacctttcacctttataggttcacatggtaatccttgttttatagattgtagttttgttttactttacttttaataaatgctatgctttctgtacgatggctacttcagGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAAC
AAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACT
TGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTA
GTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAGTATTGAGTGTTCAATATAAATGCAATAAAATGTCTGAAATGCTTCGGTTGCATTTATTC



Intron 15 GCTCAGGCTA ... ATGAGACGAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A145.54583463
A240452634
A342.49674059
A434.84723542
A535.92331819
A635.71964
A721.74361010
A80600
A932.76391424
A1038.46442431



>TRINITY_DN125058_c0_g1_i5 from sample A1 / Example spliced isoform
GTTGGAAGGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAA
AGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGA
ATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAAT
CAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCG
TTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGG
AGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGACGAGGAAATTGCTTCCCTCGAG
TGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGA
ACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTC
ATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTC
TGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGA
AGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTG
CCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACT
GAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCT
AAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGCTATGTTAAGTTTTTCCATATAGTGTTTTGCAACATTCAAAAATTGTCAGTTCATGCCTG
GGGTTTTGGAGCAGTTATTTTTCCTGCAGGCATCTTGTAGGGTAAGGAATCTGTATCAGCTGCACAGTACAAAGGGTACTATCATGTGGGTTGCTGGTAGCATGTTAACTTCAACCTATCTACACCATTGAACATGGGCCGTACACAACCCATCATATGAAACCATCTCAAGCATGTGCTGGCACATAAGTCTTTAAAAG
TGGGTGGTCAATATTCAACTCTTCTCATGTTTGTGAGATCGGAGTGGATTGAGGAGAGACTATAGAAATGTTTATGTCGAATTATGGTATATTTACTTCCAGCTTGTATTATCTTATATTGTAAACAGCACCAAGAAACGTGGCAGATACATGGACAGGAGCAGATGGGG


>TRINITY_DN125058_c0_g1_i15 from sample A1 / Example unspliced isoform:
GTTGGAAGGCGCTCAAAATTTTGGATTTTGACCGACCCAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAA
AGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGAAAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGgctcaggctaggtcgtgggtttgaattttataggagaggagtcccagaattgttgtagcatgagacgagGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAA
GCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAAT
CTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACACAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAG
AACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAG
TTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCAT
GATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGACGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTC
CTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCAC
AACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGAT
TATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGA
TGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGG
CAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGA
CTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGA
ACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATT
TCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAA
GCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTG
TAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACC



Intron 16 GTATGCTACT ... TCTTTATTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A142.86221815
A237.78281717
A346.67282623
A434.9372910.7
A534.21251610
A656.52576
A744.2617189
A860224
A938.4616128
A1039.47232010



>TRINITY_DN113498_c0_g1_i3 from sample A2 / Example spliced isoform
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGGTAGGTGCAAGTGCAAATGATCCGGTAAACGCGTAAAGGGAAATAAAGGATGAAGAATAAGGGCATTACGGCTTTTCGATTTGTTTAAACTACGGATTTGAATGAATCTGCATATTTACACCTGTAGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAG
AGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGCTCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAG
GCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACATGGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCT
CCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACTGAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAA
AATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCGTAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGT
CGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTGAAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGT
TTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTTGATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAA
AGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTCTAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGA
ATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAGAACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAAT
GACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGGGAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGG
AATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGAAGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTAT
ACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGCAGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTA
TCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCACTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGC
TCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAACATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAA
CAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGTACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGAC
TTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTATTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATT
AGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA


>TRINITY_DN113498_c0_g1_i12 from sample A2 / Example unspliced isoform:
CAGAAATAAAATAAAACAAAAAGCAAAGGCAGGGACATCCAAGGTGGCCCAGCGTGGAACCGGTTGTTCAAAATTGCACAGACCCCGATAAAGAGGAACAATTGAATTTTCAACTCTAATTGGAGGAAAAAGACGAGAGAGAGAGCGAGCGCGAGAGATTCAAAGAGGGCGTCTGTTCAATCCAGCGTTGAGTACAGGGA
AAAGCAATACGCCCCCGCTTTCTACCGGGCCAGTCCAGgtatgctacttactctactctacaatgtggacctgaaattgaaattgttgctttcataatgcctttccaacgtcactggttggaatcccgacctgcaaagatagttgataaaccggcctttcatatcccaaaggaaatgaaaggaagatttctctttcgatt
tctttattagGTAGGTGCAAGTGCAAATGATCCGGTAAACGCGTAAAGGGAAATAAAGGATGAAGAATAAGGGCATTACGGCTTTTCGATTTGTTTAAACTACGGATTTGAATGAATCTGCATATTTACACCTGTAGTAAGTGGTTTTGTGACCTGGCAGAGTCTTTTACAAAGTCTATAAGAAATCGACTTCAATTCTG
AATCTCACTGGTTTTTTGCGGGCGTCATTCGCTCGCATGCCTGAGTCCAGGCTCAGGCTAGGTCGTGGGTTTGAATTTTATAGGAGAGGAGTCCCAGAATTGTTGTAGCATGAGACGAGGACATAACGCAGCTTGAGAACTTCTGCAAAAGTAAGGTTTCAAATTTGCATTGTAAGCATGGATCCTTTTGGTGCCAATGC
TCGCTTCTCCCTGTTTTAAATGGCTACTGACTGCAGTCATTGGAATGAGACCAAGGCTCTCCCTATTGAATGTAATTGCAATTCAGGCACTGAATCAGATTATTCATGGCAGCGCTCTGTTAAGAGGAAATTTGAGGGATTAAACTTAGAATGTAATCATTCTTGTGATGAGAATCTGATTTCAGCAGAAGAGGGTACAT
GGCGTACTGCAGAGGATGTTGTCTCGGAAGATATCCATTGGAATCAATTTAATCAACAATGTAACGACACGCTTAGCAATGACTCTCCAACAAAGCCTATTGAAGGGTTTAGCAATTCTGCACATGCCCGTACAAGTTTTATATTAGGAACGTCAAATGAACGAACTCACAGTAGAACTCATGGTCCTTTTAGGCTGACT
GAGGAAAAAGAAGAACTCAAGTCTTCATGCAGCAATGAACAGCGTTCTTCATTCACTTCTGTTGTCAATGGTATTTCCCCGAAGAAAATGTTCATGGAGAAGAAAGCAGCTTCTAGAAACATGGCTGATGGAGAAACTGGGAGCCATTCTCGTATGTTAGAGGTTGAAAATGAAGTTCTCACTTTAAGAGAGGCTCTCCG
TAGCCAACATCAGGCTTTACGGGACCTTTATACGGAACTAGAGGAGGAGAGAAATTCTTCGTCTACTGCAGCAAATGAAACATTGTCGATGATTTTGCGGCTTCAAGGAGAAAAAGCTGCTGTGCGTCTAGAGGCCAATCAGTATAAAAGATTGGCCGAGGAAAAACTTGCTCATGATCAGGAATCCTTGGCATATTTTG
AAGATCTTATACATAGGAAAGGCGAGGAAATTGCTTCCCTCGAGTGTGAAGTACAAGCATACAGACACAAGTTGCTGAGTATTGGTTTTGATGATCTTGAGATTGGAAAGATTAGATGGCCTGGACGGTCTAATCCAAATGACAGAGAGGAGGATTGTGTTAGCTGGACAAGTTCCTGCACAGAAAATTTGGAGGGCTTT
GATGGCTATGATGCTCTTGGTTGGAAGACTCCAAATAATGCTGAACATTTGCATTCCAAAGATATGTTGCAGAGACAAGACAAAAAAGGCAATAGACATATTTCCATGAATGATCGCAGATGCTTAAATGATACATGGATTGATGGGGCGACTGCAGTCAATTATGGTCAGCCACAACTCCAGCGGGGAGACTTGTACTC
TAAGAGAGGTACACGTGGACAAGATAGAACCTCAGGTAACTCTCATAGGATTCAGGCAGAAGGTTACTCTGTTTCCAGAAAAATGAATAAAGAATCTACGAAAGGGTATTCAAAAGGCGAAAGCAAAAGTTATGCAAGTTACAGGATGGATTCTGATTTCGAAGTAACACAAGATTATGATATCTGTGATGTTGATCTAG
AACCTGATATTAGTACAAGTCAATTAAAAGAGCAGGATGCCGTCTGTGAAATGGCAATAGATATGCGAGAAACAGCCCTTAGCAATGACAATCTTACTCTTTGGGAACAGATAGAAAAGTTAGAGGACAGGCTGCAGCAATTATCAAAAAGAAGAGCGCATAATTGTCATGTGGATGATGAATGGGTAGCAACAAACAGG
GAGCTTCGGAAAACAGTAGTAGCTTACACTGCCAGTTTAAGGGAAGGAGCAAGGTCTCGTTGGAGACTTTCTTCAGATAACACTGGAATCTGTGATGGTTTGATGGGAACAGGTCTAGGAGAAAGTGCAGAAGATCTTCAATCATCAATTATCTTTGCAAAAAACAAGCAAATGGCAGAGGTATCTAATACTGATATTGA
AGTGCTATGTTCAACAAATAAGAGCGTGACAGATGCTAAAGCTGCCAATGATGAAATTGATTCAAATTTGAATGTGCATGATGTATACGTAGTCCAACATGAATGTGAAAGGACTGGAAGATACAAGTCTCATAAAGGAACTGCTTTTAATTTTGACGTAGCGGATGAGGATCGACTTGGAAAACCAGATCCTTTACCGC
AGAAACATCAAGAAGATAGTTCTTCCCAGAATCCTGATTTGACTGAAAGAAACTTACTGCAGTCTCCACTGCACAATGTCCAGGTATCTAAAGCAGGTGATAGTCTTTGCAATGCCTTTTCTGGTGGTTTAGCAGAGTCAATGCTTGACACTTCTCCTTTGCAAGGGCAAGTTGAACAGCTTAGCATGAGGCTGCAGGCA
CTGGAGAGTGACAGAGAGTTTATGAAACAGACCATTGATTCCCTAAAAAGAGAGAAAGAAGAATTGACATTGCTCAAGGATATAGCTCAGCAACTCCGTGATTTGAAACCACCATCCAAGAGCACCAAAGTTGTCAAAAGTCCAACCCCACAAGAGGATGCATCAGTTTTAAATTTCATGAAGGGAATTCTATCGTTCAA
CATTTTGAGAAGTAATATGCACAAGAGCACGGATGTTGGTTCACGCAACATTGTGGGCTTGTCACACCTGTTGGAGAAGACACCAACAAGGAGCATGTCCACCTGTCTTACCAGAGTTGTGAAAGTCAATTCCTTCTCAGAGGCCAGAAATGGTCATCCAGATAAAGCAATGGAAGCTGTCATGGTTCGAAACAGACTGT
ACAATGAGTAAAGGCTCTGCATCTGAATCCTCTTTCTATATTCTTTTATTAGAGGTTACAAGGCAATCTATCATGCAAGTCATGACTTGTGTGGCCTCTTTAAGATTGTTGTTTTTGCCTTGTGATTGTATACTTAATTCACGTTGCGCGGTGAATTTGTTCTCTTATATGATTGTAAGCAGGCTGACATGATCTGTTTA
TTTTGACAAAATTTACAAATATTTGGTCCCCGAGGACCATACTTTTTGTAAATGCATTCAGAGGTATGCATATATTCTGCGTCATTAGTAAGAGTAAATTTTGCCTCATTGTAAGCTTTTACAAGCATTCAATACTATTTTGGATAGACTTACTCTCTAAA