NA ----> NA

Intron 1 GTAATATATG ... AGTTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.99	140	47.3	46
A2	29.12	101	41	42
A3	25.66	113	35	43
A4	26.67	121	72	16
A5	21.11	47	15.2	10
A6	23.26	955	229	350
A7	23.77	846	189.6	338
A8	24.11	946	224	377
A9	24.46	956	225	394
A10	25.51	590	163	241

>TRINITY_DN124124_c16_g1_i3 from sample A1 / Example spliced isoform

ATTGCGTCTTAGCGTTTGAAATAATTCTCTGTTCGTCTATGCCTTTGACAATCACAAATGAACAGAAGGCTAAGATCTTGCAAGGACTGTAGAGATCCCTGCTCTGCTCATCACCCACCAGAGCTGGCGGGCTCGACTGCGCGCTCAAGGAAATAAATTCATAGGAATATTGATAATGTCATGAAGAGCATGCTTTGAGT
TAGACGATATGGAGGAATCACTTCAGCCTAATGGCCTTGTCTTGAAGAGCAATCTTGCCATTCGGGAGTTACTGGAAATGGCAGTGTCATTTGCAAATACAGATTCTTCCAAAATTCTCTCATCTGCAGGAAAATATGTCTCGTCCGATGAAGTTCCTTACACAGCAATCAGAGATATATGGTTTCAAATAGAGCCTTCT
CGAAGACCAAAATTTTCTCGTCTGCTTACCGGTGTCAGCTTTGTACTTCCAAGCCCAAAGCCTAGGGAGAAGAGTAAAGAGCTTAAGGATCGCCTAGACAGGCTTCAAGAAATGGCAGATAGGAAGGAATATGATGCACTAGTTAGAGATGTCAGCTTAAAAAAGGAAGACAAGGAATATTTTTCCTCTTTCAAAGACCA
ATTTGGATATGGTTTACATGTTATTGTAATAATGTTCACTGGCTACTTGGTTGGGTATGCGGCATTCCGAGCTTTGTTTGGACACAACCCAGTCATGAATGCCGCAGGAGGAATTTGTGGATTGGTGTCTGCTATGTTGCTTGAAACTGTATTGTTTATCATCAGAAGTTCTAGTCACCAGATGTCACCAGCGTCAACTA
CACTATCAGAAAAGCCAGATATATTTCGACCAAGAAGAAATGATAACCAAAGGTAGTATAATAGTATTGGTATAAATGTTTTTTTATTTGCTGGAACTCATCTATTGCATTTTTGTACGCTGATATTGATTTGAATAACGATTTACATGTCAAGTGTTTCTCTGGCATTAGACAAAGTCTTCCAGGTTTGAGGATTAATT
AGAAGATCAGTTTCTTTCATGATTGAGATTTTAGTATGTCTTGAAAATAAGGACTGATATGTCCATGTTATCATTTTCTATTACTTAAATGGCTATCATATATATGTTTGCACTGAGAGTAGCGAGGTGCTATACAGTCCAGTATGGGGGAGATGTTGTCATGTAACATAACGTAATGTTATGTAATGTCACCG

>TRINITY_DN124124_c16_g1_i1 from sample A1 / Example unspliced isoform:

ATTGCGTCTTAGCGTTTGAAATAATTCTCTGTTCGTCTATGCCTTTGACAATCACAAATGAACAGAAGGCTAAGATCTTGCAAGGACTGTAGAGATCCCTGCTCTGCTCATCACCCACCAGAGCTGGCGGGCTCGACTGCGCGCTCAAGGAAATAAATTCATAGGAATATTGATAATGTCATGAAGAGCATGCTTTGAGT
TAGACGATATGGAGGAATCACTTCAGCCTAATGGCCTTGTCTTGAAGAGCAATCTTGCCATTCGGGAGTTACTGGAAATGGCAGTGTCATTTGCAAATACAGATTCTTCCAAAATTCTCTCATCTGCAGGAAAATATGTCTCGTCCGATGAAGTTCCTTACACAGCAATCAGAGATATATGGTTTCAAATAGAGCCTTCT
CGAAGACCAAAATTTTCTCGTCTGCTTACCGGTGTCAGCTTTGTACTTCCAAGCCCAAAGCCTAGGGAGAAGAGTAAAGAGCTTAAGGATCGCCTAGACAGGCTTCAAGAAATGGCAGATAGGAAGGAATATGATGCACTAGTTAGAGATGTCAGCTTAAAAAAGGAAGACAAGGAATATTTTTCCTCTTTCAAAGACCA
ATTTGGATATGGTTTACATGTTATTGTAATAATGTTCACTGGCTACTTGGTTGGGTATGCGGCATTCCGAGCTTTGTTTGGACACAACCCAGTCATGgtaatatatgatgtggatattctttctatatttcttggatcaacttgctcaatatctcattttcttgtgttgtgaatcttagtggttgaagtagaaacgtgag
attagacatttttttcaagaaaagtcatcacatgaacataattttgaatccgactgaatattggttagtaactggtaagagttgtacagAATGCCGCAGGAGGAATTTGTGGATTGGTGTCTGCTATGTTGCTTGAAACTGTATTGTTTATCATCAGAAGTTCTAGTCACCAGATGTCACCAGCGTCAACTACACTATCA
GAAAAGCCAGATATATTTCGACCAAGAAGAAATGATAACCAAAGACAGTTCTCACATCATTTAAGAGAATGACATTTCAATCATGAGGCATTGACCATATTGTCTGTTTGCTACTTCTTGTTCGACATTTGTTAAACTCTAGCAATTTGCTGGCCTTTGGATATCATAGTAGCTTGCTTGCTTACACAAAATCCTTTTTT
ATTTCTAATTGCTGTGTTTTTGGTGAACATGTGTATGACCACCACATATGGTTACATTGTACTACTTTAAGTGGACTGTAAGGCAAGTAGAATTCTAAACATAGATACATCATGTATTTCACG

Intron 2 GTTTACATGT ... ATAACCAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	87.67	27	192	52
A2	84.35	41	221	33
A3	85.21	25	144	32
A4	90.79	21	207	27
A5	91.3	8	84	8
A6	87.63	163	1155	350
A7	86.18	178	1110	278
A8	84.43	194	1052	396
A9	84.26	214	1146	318
A10	84.67	163	900	181

>TRINITY_DN42465_c6_g1_i5 from sample A6 / Example spliced isoform

GGAATATATGAATCAATAATTTTTCCAAATTGCGTCTTAGCGTTTGAAATAATTCTCTGTTCGTCTATGCCTTTGACAATCACAAATGAACAGAAGGCTAAGATCTTGCAAGGACTGTAGAGATCCCTGCTCTGCTCATCACCCACCAGAGCTGGCGGGCTCGACTGCGCGCTCAAGGAAATAAATTCATAGGAATATTG
ATAATGTCATGAAGAGCATGCTTTGAGTTAGACGATATGGAGGAATCACTTCAGCCTAATGGCCTTGTCTTGAAGAGCAATCTTGCCATTCGGGAGTTACTGGAAATGGCAGTGTCATTTGCAAATACAGATTCTTCCAAAATTCTCTCATCTGCAGGAAAATATGTCTCGTCCGATGAAGTTCCTTACACAGCAATCAG
AGATATATGGTTTCAAATAGAGCCTTCTCGAAGACCAAAATTTTCTCGTCTGCTTACCGGTGTCAGCTTTGTACTTCCAAGCCCAAAGCCTAGGGAGAAGAGTAAAGAGCTTAAGGATCGCCTAGACAGGCTTCAAGAAATGGCAGATAGGAAGGAATATGATGCACTAGTTAGAGATGTCAGCTTAAAAAAGGAAGACA
AGGAATATTTTTCCTCTTTCAAAGACCAATTTGGATATGACAGTTCTCACATCATTTAAGAGAATGACATTTCAATCATGAGGCATTGACCATATTGTCTGTTTGCTACTTCTTGTTCGACATTTGTTAAACTCTAGCAATTTGCTGGCCTTTGGATATCATAGTAGCTTGCTTGCTTACACAAAATCCTTTTTTATTTC
TAATTGCTGTGTTTTTGGTGAACATGTGTATGACCACCACATATGGTTACATTGTACTACTTTAAGTGGACTGTAAGGCAAGTAGAATTCTAAACATAGATACATCATGTATTTCACGTTGTAATTTTTAAACTATTCATACTATGTATTAAGGTAACTAACGACTTTGGACATACCCGAGATACATGGGTAACTAGCCG
CTCCACCCATGCTTTTCTAAGGGAACATTCATATGTCACTTGCATCTCTACTCTTTTAACTACCGACACTTAATATTTGTTGGATCTTTGTAACATTCCCCTTGAAGACAATATCCGTTAGCTTCTTGCTTAGAGATTTAGCCAACCATTGACCGGACCACTGTGCTTGACACACTAGTATCAAC

>TRINITY_DN42465_c6_g1_i2 from sample A6 / Example unspliced isoform:

GGAATATATGAATCAATAATTTTTCCAAATTGCGTCTTAGCGTTTGAAATAATTCTCTGTTCGTCTATGCCTTTGACAATCACAAATGAACAGAAGGCTAAGATCTTGCAAGGACTGTAGAGATCCCTGCTCTGCTCATCACCCACCAGAGCTGGCGGGCTCGACTGCGCGCTCAAGGAAATAAATTCATAGGAATATTG
ATAATGTCATGAAGAGCATGCTTTGAGTTAGACGATATGGAGGAATCACTTCAGCCTAATGGCCTTGTCTTGAAGAGCAATCTTGCCATTCGGGAGTTACTGGAAATGGCAGTGTCATTTGCAAATACAGATTCTTCCAAAATTCTCTCATCTGCAGGAAAATATGTCTCGTCCGATGAAGTTCCTTACACAGCAATCAG
AGATATATGGTTTCAAATAGAGCCTTCTCGAAGACCAAAATTTTCTCGTCTGCTTACCGGTGTCAGCTTTGTACTTCCAAGCCCAAAGCCTAGGGAGAAGAGTAAAGAGCTTAAGGATCGCCTAGACAGGCTTCAAGAAATGGCAGATAGGAAGGAATATGATGCACTAGTTAGAGATGTCAGCTTAAAAAAGGAAGACA
AGGAATATTTTTCCTCTTTCAAAGACCAATTTGGATATGgtttacatgttattgtaataatgttcactggctacttggttgggtatgcggcattccgagctttgtttggacacaacccagtcatgaatgccgcaggaggaatttgtggattggtgtctgctatgttgcttgaaactgtattgtttatcatcagaagttct
agtcaccagatgtcaccagcgtcaactacactatcagaaaagccagatatatttcgaccaagaagaaatgataaccaaagACAGTTCTCACATCATTTAAGAGAATGACATTTCAATCATGAGGCATTGACCATATTGTCTGTTTGCTACTTCTTGTTCGACATTTGTTAAACTCTAGCAATTTGCTGGCCTTTGGATAT
CATAGTAGCTTGCTTGCTTACACAAAATCCTTTTTTATTTCTAATTGCTGTGTTTTTGGTGAACATGTGTATGACCACCACATATGGTTACATTGTACTACTTTAAGTGGACTGTAAGGCAAGTAGAATTCTAAACATAGATACATCATGTATTTCACGTTGTAATTTTTAAACTATTCATACTATGTATTAAGGTAACT
AACGACTTTGGACATACCCGAGATACATGGGTAACTAGCCGCTCCACCCATGCTTTTCTAAGGGAACATTCATATGTCACTTGCATCTCTACTCTTTTAACTACCGACACTTAATATTTGTTGGATCTTTGTAACATTCCCCTTGAAGACAATATCCGTTAGCTTCTTGCTTAGAGATTTAGCCAACCATTGACCGGACC
ACTGTGCTTGACACACTAGTATCAAC