NA ----> NA

Intron 1 GTATGTTCCA ... AATCACGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.26	11	13	19
A2	38.46	8	6	4
A3	53.85	3	3	4
A4	62.16	7	13	10
A5	100	0	1	1
A6	65.52	10	16	22
A7	65.38	18	36	32
A8	57.61	22	30	29.8
A9	63.41	15	24	28
A10	81.13	10	43	43

>TRINITY_DN112815_c0_g1_i4 from sample A1 / Example spliced isoform

CCTGGGTGAGAAAATGGCTAATACCCTTCAGTCCAAAACCCACATTTTTTTAAACTTACGAAAAATAATACAACTGTGAACACCATATCATTTTCCATCATTGCGCTTCAGCCTAGCATGTATCCCAGACTTTATGACTGTTTCAAAGAGTAATACACAAGACTGGTGGAAATGGATATACAATAAAATTCAATTTTCAT
CATATGACATGTGACTTTTTTGTTCTTCTCAGCTGTTGAAACAGACAGCAATGGCCTCAAAATTCAATGGCATTGTAACGCTGCCTTCTCCTTCTCTAGCGGTTCTTGGATGTCAGAGAATTCGAAATCAGCAACAACTATGCAATCGAAATGTATGCAGAAGACTGAAAAATAGGTCACCCTGTCCGCAATGGACGGAT
GCTAGACCTCAACAAATTCTTCAGCTTCAAGAGTTTTATCATCCGAGGTATGCTCCAAGAAACTGTGTTTCAGAAGAGTCCACGGATTCCTCAGCGGAAAGTGTATCTGAGATCAATTTCTACATGGGGGATGTCAATGAATTTGAAGAAGAGATAGAGGAGCCTGCGAAATGCAGAAATGATTGGCAAAACGGAAATTG
TACTGTATGCAATGAAAGATTTTCTTTGTTGCTTGCCGAAAAAACATGAAGCAGATTGGAAGATCTGTAAAAAGGTTTGGAAGCTGTTCAGTAGATCTGTGGAGTTCCAGGATCCTCTAATTTGTTTTCGTGGTATTAAGAGGTCATGTCCATCCCATCTCTAAGGAAAAATGGAAGCAAGCAAGAGAATACAGTCAGGA
GAAGGACGATACAACAAAGATTTTTGGTGAAAAGCCTACACTAGAGCAGATCATGGAGAATTACAAAGGTGTTGTCTATCAGAGGAGGAAGAAGATCATCCCAACTAAATCAGATCAACACATGCCGAAGGAACCAAAGATTCTAGGTTTCTCGTACGATCGATTTCCACGAAGTTCAGATCAAT

>TRINITY_DN112815_c0_g1_i2 from sample A1 / Example unspliced isoform:

CCTGGGTGAGAAAATGGCTAATACCCTTCAGTCCAAAACCCACATTTTTTTAAACTTACGAAAAATAATACAACTGTGAACACCATATCATTTTCCATCATTGCGCTTCAGCCTAGCATGTATCCCAGACTTTATGACTGTTTCAAAGAGTAATACACAAGACTGGTGGAAATGGATATACAATAAAATTCAATTTTCAT
CATATGACATGTGACTTTTTTGTTCTTCTCAGCTGTTGAAACAGACAGCAATGGCCTCAAAATTCAATGGCATTGTAACGCTGCCTTCTCCTTCTCTAGCGGTTCTTGGATGTCAGAGAATTCGAAATCAGCAACAACTATGCAATCGAAATGTATGCAGAAGACTGAAAAATAGGTCACCCTGTCCGCAATGGACGGAT
GCTAGACCTCAACAAATTCTTCAGCTTCAAGAGTTTTATCATCCGAGGTATGCTCCAAGAAACTGTGTTTCAGAAGAGTCCACGGATTCCTCAGCGGAAAGTGTATCTGAGATCAATTTCTACATGGGGGATGTCAATGAATTTGAAGAAGAGATAGAGGAGCCTGCGAAgtatgttccacctccacctttaaatcacga
agATGCAGAAATGATTGGCAAAACGGAAATTGTACTGTATGCAATGAAAGATTTTCTTTGTTGCTTGCCGAAAAAACATGAAGCAGATTGGAAGATCTGTAAAAAGGTTTGGAAGCTGTTCAGTAGATCTGTGGAGTTCCAGGATCCTCTAATTTGTTTTCGTGGTATTAAGAGTTTAATATGGTGGCTGGAAGCTGTGC
GTGATGCGGTGATTTTAGTGGATGTGGATATAAATGGGATCTATAGTGTGATTGAGGAAAAAGGTGATGGTAAGTTTTGACTCGATTGTTAAATGTGGAAAATGTTTGTGACCAGTTCTATTGTAGTTATAAGCTTTTCGTTTAGCTCCA

Intron 2 GTGAGACAAA ... TTTAGGCGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.96	5	11	6
A2	-1	0	0	0
A3	45.45	3	3	2
A4	73.33	2	6	5
A5	50	1	1	1
A6	50.94	13	17	10
A7	36.11	23	14	12
A8	51.02	24	30	20
A9	72.5	11	37	21
A10	60.87	9	16	12

>TRINITY_DN36043_c0_g1_i8 from sample A9 / Example spliced isoform

TTGCAGACTTTTTGAATTGTCTGATTGAGTCTATTCACACAAGTTGGCCCTCACTAAGAGATGAACCAGAGTAATTACAAGGGTATGCCAAACCAGTGCCAGTTGCTCTGCTGTCTAGAAAATCTTGAAGGCTTATGTGGATGTATTAAGACTCGAGGGATTGTCTGTTACCTTTGGTTCTACACAAAGATTAGAAAGAT
CTCGATGGATAATTATGTTGGATTTTCCATTTGGCTCTCTCTCTCTCTTTGACTAAT

>TRINITY_DN36043_c0_g1_i3 from sample A9 / Example unspliced isoform:

GTGAGAAAATGGCTAATACCCTTCAGTCCAAAACCCACATTTTTTTAAACTTACGAAAAATAATACAACTGTGAACACCATATCATTTTCCATCATTGCGCTTCAGCCTAGCATGTATCCCAGACTTTATGACTGTTTCAAAGAGTAATACACAAGACTGGTGGAAATGGATATACAATAAAATTCAATTTTCATCATAT
GACATGTGACTTTTTTGTTCTTCTCAGCTGTTGAAACAGACAGCAATGGCCTCAAAATTCAATGGCATTGTAACGCTGCCTTCTCCTTCTCTAGCGGTTCTTGGATGTCAGAGAATTCGAAATCAGCAACAACTATGCAATCGAAATGTATGCAGAAGACTGAAAAATAGGTCACCCTGTCCGCAATGGACGGATGCTAG
ACCTCAACAAATTCTTCAGCTTCAAGAGTTTTATCATCCGAGGTATGCTCCAAGAAACTGTGTTTCAGAAGAGTCCACGGATTCCTCAGCGGAAAGTGTATCTGAGATCAATTTCTACATGGGGGATGTCAATGAATTTGAAGAAGAGATAGAGGAGCCTGCGAAATGCAGAAATGATTGGCAAAACGGAAATTGTACTG
TATGCAATGAAAGATTTTCTTTGTTGCTTGCCGAAAAAACATGAAGCAGATTGGAAGATCTGTAAAAAGGTTTGGAAGCTGTTCAGTAGATCTGTGGAGTTCCAGGATCCTCTAATTTGTTTTCGTGGTATTAAGAGTTTAATATGGTGGCTGGAAGCTGTGCGTGATGCGGTGATTTTAGTGGATGTGGATATAAATGG
GATCTATAGTGTGATTGAGGAAAAAGGTGATGCTAAGCCACAGATCTACATAATATGGACAGTGGCATTAACAAAAGATCGACAAGAGAGTTTTGTAAACCTTGTTAGACAGGATTGGTGTAGAAAAGAATATGGGACATTACTGCAATACCTTCATAATCAGAAGGAAGTTAGCAAGAACACCTGTCAATCTTTGCTTG
CCAATGAAGATCTGAAAGAAATGGGTCAGGAATCAACCTCAAGTTTGGATGATAATCGAGACCTACAGGAGCACAGTTGCAGACTTTTTGAATTGTCTGgtgagacaaagctaggagttaactctttaggcgagATTGAGTCTATTCACACAAGTT

Intron 3 GTATGTTCCA ... GTATTAAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.49	9	13	28
A2	60	4	6	6
A3	100	0	3	11
A4	92.31	1	13	11
A5	100	0	1	3
A6	66.67	11	16	28
A7	75.7	13	36	45
A8	82.52	9	30	55
A9	78.64	11	24	57
A10	84.31	8	43	43

>TRINITY_DN38413_c0_g1_i2 from sample A6 / Example spliced isoform

GAAGAGATAGAGGAGCCTGCGAATTTAATATGGTGGCTGGAAGCTGTGCGTGATGCGGTGATTTTAGTGGATGTGGATATAAATGGGATCTATAGTGTGATTGAGGAAAAAGGTGATGCTAAGCCACAGATCTACATAATATGGACAGTGGCATTAACAAAAGATCGACAAGAGAGTTTTGTAAACCTTGTTAGACAGGA
TTGGTGTAGAAAAGAATATGGGACATTACTGCAATACCTTCATAATCAGAAGGAAGTTAGCAAGAACACCTGTCAATCTTTGCTTGCCAATGAAGATCTGAAAGAAATGGGTCAGGAATCAACCTCAAGTTTGGATGATAATCGAGACCTACAGGAGCACAGTTGCAGACTTTTTGAATTGTCTGGTGAGACAAAGCTAG
GAGTTAACTCTTTAGGCGAGATTGAGTCTATTCACACAAGTTGGCCCTCACTAAGAGATGAACCAGAGTAATTACAAGGGTATGCCAAACCAGTGCCAGTTGCTCTGCTGTCTAGAAAATCTTGAAGGCTTATGTGGATGTATTAAGACTCGAGGGATTGTCTGTTACCTTTGGTTCTACACAAAGATTAGAAAGATCTC
GATGGATAATTATGTTGGATTTTCCATTTGGTGCTCTCTCTCTCTTTGACTAATAATAGTTTAGTTGCATCTAACATCTTACAGGAATTCGCTTTATATGTAAACATCTTGGTCCCATAGGCCAGTTGGCCTGGCAGATGGATTAATAAAACCTTTCTTTCTATAACGTAGATTTTGTTACAATACCCAATACATGCACA
ATTCTACACCCCTTTACTTGGAATTATTACAATGAATTTGAATAGGATAAATGGCAATGAGGTGATTGGAGTTTCTGACTGACCCTGCTAAAGAGTTATGGCCATTATGAAATTGTTAATTGCCTGGATAAAATTACTGTCATATGTATGGTAATTAATTCCATTTATTATAAGAGTTGTCCATGTCTTAAACGTGGAAA
CTGTTCAGTTATTATTTCATAATATTTAAACAATACTAT

>TRINITY_DN38413_c0_g1_i4 from sample A6 / Example unspliced isoform:

AAAAAATGTGTGCTTTGTTTTCCCGTGTTCACATTCACTTTCCCAGCCTGGCTGTTGAAACAGACAGCAATGGCCTCAAAATTCAATGGCATTGTAACGCTGCCTTCTCCTTCTCTAGCGGTTCTTGGATGTCAGAGAATTCGAAATCAGCAACAACTATGCAATCGAAATGTATGCAGAAGACTGAAAAATAGGTCACC
CTGTCCGCAATGGACGGATGCTAGACCTCAACAAATTCTTCAGCTTCAAGAGTTTTATCATCCGAGGTATGCTCCAAGAAACTGTGTTTCAGAAGAGTCCACGGATTCCTCAGCGGAAAGTGTATCTGAGATCAATTTCTACATGGGGGATGTCAATGAATTTGAAGAAGAGATAGAGGAGCCTGCGAAgtatgttccac
ctccacctttaaatcacgaagatgcagaaatgattggcaaaacggaaattgtactgtatgcaatgaaagattttctttgttgcttgccgaaaaaacatgaagcagattggaagatctgtaaaaaggtttggaagctgttcagtagatctgtggagttccaggatcctctaatttgttttcgtggtattaagagTTTAATA
TGGTGGCTGGAAGCTGTGCGTGATGCGGTGATTTTAGTGGATGTGGATATAAATGGGATCTATAGTGTGATTGAGGAAAAAGGTGATGCTAAGCCACAGATCTACATAATATGGACAGTGGCATTAACAAAAGATCGACAAGAGAGTTTTGTAAACCTTGTTAGACAGGATTGGTGTAGAAAAGAATATGGGACATTACT
GCAATACCTTCATAATCAGAAGGAAGTTAGCAAGAACACCTGTCAATCTTTGCTTGCCAATGAAGATCTGAAAGAAATGGGTCAGGAATCAACCTCAAGTTTGGATGATAATCGAGACCTACAGGAGCACAGTTGCAGACTTTTTGAATTGTCTGGTGAGACAAAGCTAGGAGTTAACTCTTTAGGCGAGATTGAGTCTA
TTCACACAAGTTGGCCCTCACTAAGAGATGAACCAGAGTAATTACAAGGGTATGCCAAACCAGTGCCAGTTGCTCTGCTGTCTAGAAAATCTTGAAGGCTTATGTGGATGTATTAAGACTCGAGGGATTGTCTGTTACCTTTGGTTCTACACAAAGATTAGAAAGATCTCGATGGATAATTATGTTGGATTTTCCATTTG
GTGCTCTCTCTCTCTTTGACTAATAATAGTTTAGTTGCATCTAACATCTTACAGGAATTCGCTTTATATGTAAACATCTTGGTCCCATAGGCCAGTTGGCCTGGCAGATGGATTAATAAAACCTTTCTTTCTATAACGTAGATTTTGTTACAATACCCAATACATGCACAATTCTACACCCCTTTACTTGGAATTATTAC
AATGAATTTGAATAGGATAAATGGCAATGAGGTGATTGGAGTTTCTGACTGACCCTGCTAAAGAGTTATGGCCATTATGAAATTGTTAATTGCCTGGATAAAATTACTGTCATATGTATGGTAATTAATTCCATTTATTATAAGAGTTGTCCATGTCTTAAACGTGGAAACTGTTCAGTTATTATTTCATAATATTTAAA
CAATACTAT

Intron 4 GTGAGAAAAT ... TTCTTCTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70.45	13	4	31
A2	75	2	3	6
A3	80.95	4	0	17
A4	60	10	10	15
A5	66.67	6	1	12
A6	63.33	11	3	19
A7	77.36	12	2	41
A8	61.11	7	7	11
A9	66.67	17	8	34
A10	72.97	10	6	27

>TRINITY_DN93510_c0_g1_i4 from sample A4 / Example spliced isoform

GTGCTTTGTTTTCCCGTGTTCACATTCACTTTCCCAGCCTGGCTGTTGAAACAGACAGCAATGGCCTCAAAATTCAATGGCATTGTAACGCTGCCTTCTCCTTCTCTAGCGGTTCTTGGATGTCAGAGAATTCGAAATCAGCAACAACTATGCAATCGAAATGTATGCAGAAGACTGAAAAATAGGTCACCCTGTCCGCA
ATGGACGGATGCTAGACCTCAACAAATTCTTCAGCTTCAAGAGTTTTATCATCCGAGGTATGCTCCAAGAAACTGTGTTTCAGAAGAGTCCACGGATTCCTCAGCGGAAAGTGTATCTGAGATCAATTTCTACATGGGGGATGTCAATGAATTTGAAGAAGAGATAGAGGAGCCTGCGAAGTATGTTCCACCTCCACCTT
TAAATCACGAAGATGCAGAAATGATTGGCAAAACGGAAATTGTACTGTATGCAATGAAAGATTTTCTTTGTTGCTTGCCGAAAAAACATGAAGCAGATTGGAAGATCTGTAAAAAGGTTTGGAAGCTGTTCAGTAGATCTGTGGAGTTCCAGGATCCTCTAATTTGTTTTCGTGGTATTAAGAGTTTAATATGGTGGCTG
GAAGCTGTGCGTGATGCGGTGATTTTAGTGGATGTGGATATAAATGGGATCTATAGTGTGATTGAGGAAAAAGGTGATGCTAAGCCACAGATCTACATAATATGGACAGTGGCATTAACAAAAGATCGACAAGAGAGTTTTGTAAACCTTGTTAGACAGGATTGGTGTAGAAAAGAATATGGGACATTACTGCAATACCT
TCATAATCAGAAGGAAGTTAGCAAGAACACCTGTCAATCTTTGCTTGCCAATGAAGATCTGAAAGAAATGGGTCAGGAATCAACCTCAAGTTTGGATGATAATCGAGACCTACAGGAGCACAGTTGCAGACTTTTTGAATTGTCTGGTGAGACAAAGCTAGGAGTTAACTCTTTAGGCGAGATTGAGTCTATTCACACAA
GTTGGCCCTCACTAAGAGATGAACCAGAGTAATTACAAGGGTATGCCAAACCAGTGCCAGTTGCTCTGCTGTCTAGAAAATCTTGAAGGCTTATGTGGATGTATTAAGACTCGAGGGATTGTCTGTTACCTTTGGTTCTACACAAAGATTAGAAAGATCTCGATGGATAATTATGTTGGATTTTCCATTTGGTGCTCTCT
CTCTCTTTGACTAATAATAGTTTAGTTGCATCTAACATCTTACAGGAATTCGCTTTATATGTAAACATCTTGG

>TRINITY_DN93510_c0_g1_i2 from sample A4 / Example unspliced isoform:

GTGCTTTGTTTTCCCGTGTTCACATTCACTTTCCCAGCCTGGgtgagaaaatggctaatacccttcagtccaaaacccacatttttttaaacttacgaaaaataatacaactgtgaacaccatatcattttccatcattgcgcttcagcctagcatgtatcccagactttatgactgtttcaaagagtaatacacaagac
tgatggaaatggatatacaataaaattcaattttcatcatatgacatgtgacttttttgttcttctcagCTGTTGAAACAGACAGCAATGGCCTCAAAATTCAATGGCATTGTAACGCTGCCTTCTCCTTCTCTAGCGGTTCTTGGATGTCAGAGAATTCGAAATCAGCAACAACTATGCAATCGAAATGTATGCAGAAG
ACTGAAAAATAGGTCACCCTGTCCGCAATGGACGGATGCTAGACCTCAACAAATTCTTCAGCTTCAAGAGTTTTATCATCCGAGGTATGCTCCAAGAAACTGTGTTTCAGAAGAGTCCACGGATTCCTCAGCGGAAAGTGTATCTGAGATCAATTTCTACATGGGGGATGTCAATGAATTTGAAGAAGAGATAGAGGAGC
CTGCGAAGTATGTTCCACCTCCACCTTTAAATCACGAAGATGCAGAAATGATTGGCAAAACGGAAATTGTACTGTATGCAATGAAAGATTTTCTTTGTTGCTTGCCGAAAAAACATGAAGCAGATTGGAAGATCTGTAAAAAGGTTTGGAAGCTGTTCAGTAGATCTGTGGAGTTCCAGGATCCTCTAATTTGTTTTCGT
GGTATTAAGAGGTCATGTCCATCCCATCTCTAAGGAAAAATGGAAGCAAGCAAGAGAATACAGTCAGGAGAAGG