sp|P69309|UBIQP_AVEFA ----> Polyubiquitin

Intron 1 GTATTCTGCT ... TTTGGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22	797.5	21	225
A2	22.34	890	11.5	256
A3	22.02	935	9	264
A4	23.63	924.5	15.5	286
A5	20.41	557.5	11.5	143
A6	21.83	612.5	8.5	171
A7	19.89	1695	20.5	421
A8	20.8	506.5	3.5	133
A9	19.14	1668.5	16	395
A10	18.13	1864	10.5	413

>TRINITY_DN44082_c1_g3_i3 from sample A8 / Example spliced isoform

GAGCAGGCCAGTTATAGTAAGGAAGGAAGAAAGCAAAAAAGGTCTGCTGCCTCTATCTCCGTCGTATAATTTATGCAGGTTAGATTTGTTTTGCACAATTTGTTGCTAATATGCAGATATTTGTGAGGTCATATACTGGAAAGACTATAGTTCTGGTGGTAGAGAGTTCTGACACGATAGATAATGTAAAATCCAAGATT
CAAGACAAGGAATGCATTCCTGCTGACCAGCAAAGACTGATATTTGGTGGAAAGCAGTTGGAGGATGGACAGACCCTTGCTTATTACAATATCGATACGGATTCTACACTTCAAGTGGATCTTCGTCTCAGAGGTGGAATGCAAATCTTTGTTAAGACTTTGACTGGCAAAATCATAACTCTTGAGGTTGAGAGTGCGGA
AACCATTGAGAAGGTCAAGGCAAAGATTCAAGAAAAGGATGGTGCACCTCTCGATCAGCAACAGGTGATCTTTGCTGGCAAGCAACTGGAGGATGGCCGTACTTTAGCTGATTATAATATCCAGAAGGAATCAACTCTTCACTTGGATCTGCGTTTGAGAGGCGGAATGCAGATCTTTGTGAAGACCTTGACCGGTAAGA
CTATCACTCTTGAAGTTGAGAGTTCTGACACAATTGACAATGTCAAGGCCAAGATCCAGGATAAAGAGGGAATTCCTCCAGATCAGCAGAGGTTGATATTTGCTGGGAAGCAACTTGAAGATGGAAGGAAGACTATCACTCTTGAAGTTGAGAGTTCTGACACAATTGACAATGTCAAGGCCAAGATTCAGGATAAAGAG
GGAGTTCCTCCAGATCAGCAGAGGTTGATATATGCTGGAAAGCAACTTGAAGATGGAAGAACTCTTGCAGATTACAATATTCAGAAGGAATCGACCCTCCACCTGGTTCTTCGGCTGCGTGGTGGTATGCAGATTTTTGTGAAGACATTGACTGGCAAGACTATCACTCTTGAAGTTGAGAGTTCTGACACAATTGACAA
TGTCAAGGCCAAGATTCAGGATAAAGAGGGAATTCCTCCAGATCAGCAGAGGTTAATATTTGCTGGGAAGCAACTTGAAGATGGAAGGACTCTTGCAGATTACAATATTCAGAAGGAATCGACCCTCCACCTTGTTCTTCGG

>TRINITY_DN44082_c1_g3_i4 from sample A8 / Example unspliced isoform:

GAGCAGGCCAGTTATAGTAAGGAAGGAAGAAAGCAAAAAAGGTCTGCTGCCTCTATCTCCGTCGTATAATTTATGCAGgtattctgcttctcttctgtgtgcaagattggtagtctgttcaaatttaaagccacaatttgttgaaccattaaatgctcaatttctgcgtgctatcccacaaaatcgatggtctgtgttct
gtttttcctttatatgtatagtccatgcaatggtggagtttgcctatttctttacttcggcattaacataatatgaatatatatgcctgacctcatgttgaacgtttagtttcctaatggtttccggttaaaccccagatttcggggttagaaaccctgagaatttacatttttatactctgcattctcttgatttcaac
tacaaatttcgggggttagctactgaggttcaagggttggtattcatattcttttggcgttggttactggtcaaatttggcaagcagagaattcgggtcttgctagttttcctccatactatattacaaatagccaatttcaaagttattgataatctgtgtagagtttaatatacagtcctgaagagtagacaatttgg
aatccttttgtaaactactgtatttgttagaggagtttcccatgaacattttgccatcgactttcataagagtttgatctcatgatctcagcatcgtttggtagtttggagttaaaaccttcttctgcctttacattaaaacctgatattggaagtgaagttgatttggagtattttgggttagagaaggaaaagttgtc
tgtcttgtctgaattttttttattgtataagttattgacttctcgtcatatccttttttggcacagGTTAGATTTGTTTTGCACAATTTGTTGCTAATATGCAGATATTTGTGAGGTCATATACTGGAAAGACTATAGTTCTGGTGGTAGAGAGTTCTGACACGATAGATAATGTAAAATCCAAGATTCAAGACAAGGAA
TGCATTCCTGCTGACCAGCAAAGACTGATATTTGGTGGAAAGCAGTTGGAGGATGGACAGACCCTTGCTTATTACAATATCGATACGGATTCTACACTTCAAGTGGATCTTCGTCTCAGAGGTGGAATGCAAATCTTTGTTAAGACTTTGACTGGCAAAATCATAACTCTTGAGGTTGAGAGTGCGGAAACCATTGAGAA
GGTCAAGGCAAAGATTCAAGAAAAGGATGGTGCACCTCTCGATCAGCAACAGGTGATCTTTGCTGGCAAGCAACTGGAGGATGGCCGTACTTTAGCTGATTATAATATCCAGAAGGAATCAACTCTTCACTTGGATCTGCGTTTGAGAGGCGGAATGCAGATCTTTGTGAAGACCTTGACCGGTAAGACTATCACTCTTG
AAGTTGAGAGTTCTGACACAATTGACAATGTCAAGGCCAAGATCCAGGATAAAGAGGGAATTCCTCCAGATCAGCAGAGGTTGATATTTGCTGGGAAGCAACTTGAAGATGGAAGGAAGACTATCACTCTTGAAGTTGAGAGTTCTGACACAATTGACAATGTCAAGGCCAAGATTCAGGATAAAGAGGGAGTTCCTCCA
GATCAGCAGAGGTTGATATATGCTGGAAAGCAACTTGAAGATGGAAGAACTCTTGCAGATTACAATATTCAGAAGGAATCGACCCTCCACCTGGTTCTTCGGCTGCGTGGTGGTATGCAGATTTTTGTGAAGACATTGACTGGCAAGACTATCACTCTTGAAGTTGAGAGTTCTGACACAATTGACAATGTCAAGGCCAA
GATTCAGGATAAAGAGGGAATTCCTCCAGATCAGCAGAGGTTAATATTTGCTGGGAAGCAACTTGAAGATGGAAGGACTCTTGCAGATTACAATATTCAGAAGGAATCGACCCTCCACCTTGTTCTTCGG

Intron 2 GTCTGTGTTC ... TTTGGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	225	22	225
A2	50.49	251	9	256
A3	50.19	262	6	264
A4	50.89	276	10	286
A5	51.07	137	23	143
A6	50.59	167	11	171
A7	51.53	396	19	421
A8	51.95	123	10	133
A9	50.58	386	6	395
A10	50.24	409	14	413

>TRINITY_DN40765_c1_g1_i2 from sample A9 / Example spliced isoform

ATTTGCCTCTACAAATACTAATCTCGGCAACCTCGCTCGGCCTCATGTGTTATGAGCAGGCCAGTTATAGTAAGGAAGGAAGAAAGCAAAAAAGGTCTGCTGCCTCTATCTCCGTCGTATAATTTATGCAGGTATTCTGCTTCTCTTCTGTGTGCAAGATTGGTAGTCTGTTCAAATTTAAAGCCACAATTTGTTGAACC
ATTAAATGCTCAATTTCTGCGTGCTATCCCACAAAATCGATGGTTAGATTTGTTTTGCACAATTTGTTGCTAATATGCAGATATTTGTGAGGTCATATACTGGAAAGACTATAGTTCTGGTGGTAGAGAGTTCTGACACGATAGATAATGTAAAATCCAAGATTCAAGACAAGGAATGCATTCCTGCTGACCAGCAAAGA
CTGATATTTGGTGGAAAGCAGTTGGAGGATGGACAGACCCTTGCTTATTACAATATCGATACGGATTCTACACTTCAAGTGGATCTTCGTCTCAGAGGTGGAATGCAAATCTTTGTTAAGACTTTGACTGGCAAAATCATAACTCTTGAGGTTGAGAGTGCGGAAACCATTGAGAAGGTCAAGGCAAAGATTCAAGAAAA
GGATGGTGCACCTCTCGATCAGCAACAGGTGATCTTTGCTGGCAAGCAACTGGAGGATGGCCGTACTTTAGCTGATTATAATATCCAGAAGGAATCAACTCTTCACTTGGATCTGCGTTTGAGAGGCGGAATGCAGATCTTTGTGAAGACCTTGACCGGTAAGACTATCACTCTTGAAGTTGAGAGTTCTGACACAATTG
ACAATGTCAAGGCCAAGATCCATCGGGATAAAGAGGGAATTCCTCCAGATCAGCAGAGGTTAATATTTGCTGGGAAGCAACTTGAAGATGGAAGGACTCTTGCAGATTACAATATTCAGAAGGAATCGACCCTCCACCTTGTTCTTCGGCTGCGTGGTGGTACTCCACCTTGTTCTACGGCTGCGTGGTGGCATGCAGAT
TTTTGTGAAGACATTGACTGGCAAGACTATCACTCTTGAAGTTGAGAGTTCTGACACAATTGACAATGTCAAGGCCAAGATTCAGGATAAAGAGGGAATTCCTCCAGATCAGCAGAGGTTGATATATGCTGGAAAGCAACTTGAAGATGGAAGAACTCTTGCAGATTACAATATTCAGAAGGAATCGACCCTCCACCTTG
TTCTTCGGTTGCGTGGTGGTATGCAGATTTTCGTCAAACTCCACCTTGTTCTACGGCTGCGTGGTGGTATGCAGATTTTTGTGAAGACATTGACTGGCAAGACTATCACTCTTGAAGTTGAGAGTTCTGACACAATTGACAATGTCAAGGCCAAGATTCGGGATAAAGAGGGAA

>TRINITY_DN40765_c1_g1_i6 from sample A9 / Example unspliced isoform:

ATTTGCCTCTACAAATACTAATCTCGGCAACCTCGCTCGGCCTCATGTGTTATGAGCAGGCCAGTTATAGTAAGGAAGGAAGAAAGCAAAAAAGGTCTGCTGCCTCTATCTCCGTCGTATAATTTATGCAGGTATTCTGCTTCTCTTCTGTGTGCAAGATTGGTAGTCTGTTCAAATTTAAAGCCACAATTTGTTGAACC
ATTAAATGCTCAATTTCTGCGTGCTATCCCACAAAATCGATGgtctgtgttctgtttttcctttatatgtatagtccatgcaatggtggagtttgcctatttctttacttcggcattaacataatatgaatatatatgcctgacctcatgttgaacgtttagtttcctaatggtttccggttaaaccccagatttcgggg
ttagaaaccctgagaatttacatttttatactctgcattctcttgatttcaactacaaatttcgggggttagctactgaggttcaagggttggtattcatattcttttggcgttggttactggtcaaatttggcaagcagagaattcgggtcttgctagttttcctccatactatattacaaatagccaatttcaaagtt
attgataatctgtgtagagtttaatatacagtcctgaagagtagacaatttggaatccttttgtaaactactgtatttgttagaggagtttcccatgaacattttgccatcgactttcataagagtttgatctcatgatctcagcatcgtttggtagtttggagttaaaaccttcttctgcctttacattaaaacctgat
attggaagtgaagttgatttggagtattttgggttagagaaggaaaagttgtctgtcttgtctgaattttttttattgtataagttattgacttctcgtcatatccttttttggcacagGTTAGATTTGTTTTGCACAATTTGTTGCTAATATGCAGATATTTGTGAGGTCATATACTGGAAAGACTATAGTTCTGGTGG
TAGAGAGTTCTGACACGATAGATAATGTAAAATCCAAGATTCAAGACAAGGAATGCATTCCTGCTGACCAGCAAAGACTGATATTTGGTGGAAAGCAGTTGGAGGATGGACAGACCCTTGCTTATTACAATATCGATACGGATTCTACACTTCAAGTGGATCTTCGTCTCAGAGGTGGAATGCAAATCTTTGTTAAGACT
TTGACTGGCAAAATCATAACTCTTGAGGTTGAGAGTGCGGAAACCATTGAGAAGGTCAAGGCAAAGATTCAAGAAAAGGATGGTGCACCTCTCGATCAGCAACAGGTGATCTTTGCTGGCAAGCAACTGGAGGATGGCCGTACTTTAGCTGATTATAATATCCAGAAGGAATCAACTCTTCACTTGGATCTGCGTTTGAG
AGGCGGAATGCAGATCTTTGTGAAGACCTTGACCGGTAAGACTATCACTCTTGAAGTTGAGAGTTCTGACACAATTGACAATGTCAAGGCCAAGATCCATCGGGATAAAGAGGGAATTCCTCCAGATCAGCAGAGGTTAATATTTGCTGGGAAGCAACTTGAAGATGGAAGGACTCTTGCAGATTACAATATTCAGAAGG
AATCGACCCTCCACCTTGTTCTTCGGCTGCGTGGTGGTACTCCACCTTGTTCTACGGCTGCGTGGTGGCATGCAGATTTTTGTGAAGACATTGACTGGCAAGACTATCACTCTTGAAGTTGAGAGTTCTGACACAATTGACAATGTCAAGGCCAAGATTCAGGATAAAGAGGGAATTCCTCCAGATCAGCAGAGGTTGAT
ATATGCTGGAAAGCAACTTGAAGATGGAAGAACTCTTGCAGATTACAATATTCAGAAGGAATCGACCCTCCACCTTGTTCTTCGGTTGCGTGGTGGTATGCAGATTTTCGTCAAACTCCACCTTGTTCTACGGCTGCGTGGTGGTATGCAGATTTTTGTGAAGACATTGACTGGCAAGACTATCACTCTTGAAGTTGAGA
GTTCTGACACAATTGACAATGTCAAGGCCAAGATTCGGGATAAAGAGGGAA