NA ----> NA

Intron 1 GTAGGCAAGT ... TCAAAGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75.76	8	25	2
A2	57.69	11	15	3
A3	65.52	10	19	2
A4	53.13	15	17	1
A5	60	10	15	2
A6	61.76	13	21	3
A7	72.22	20	52	17
A8	67.31	17	35	9
A9	60.53	30	46	7
A10	73.13	18	49	13

>TRINITY_DN36130_c0_g1_i2 from sample A8 / Example spliced isoform

CTCCAAAGCAAATTCAGAAGTACTCAATTTTATATCATTTTTATGGGTTTTTATAGCGAATTAGATTTTTATATTTGTGGAGGCTGTTCATCTATGTCACATACTCAGAATCTGTTTACCTATTAATAGGCTATATTATGAATTCAAAAATTTCACTGCAGTGAATTTTATTGTCAGTTGTATGGGTGATTGCAGGCAGA
ATACATGGACTTGAAAACCTTGCATTCCCGTGTAGAAGATGCTGTAAATACCATGATTCGAAGAGATGAAAGTACTGAAAATGGTCAATTTTTGAAACCTTGTGTTGAAGCGGCTCTCTATGTGGGATGTCCACCAAGGAAAGCACCTAGGAGTCAGAGGCATAATTCACGATTCTGTGCAGGCAGCAGCCAACGACTTA
CTACATCCTCTTCTGTCCCTGTGGCATGTTGCTGATGTCGGGTGTGACTACCATGTACTCTTAAATGCTAGCTGGAGCGAAATCAGGCAGTCAGAGTAACTTGTTCTCACATTCAAAGAGAGTGGCTAAAATTGCTGAGGCCTCCGCTTGTATTCCTGATGAATTCACTTAACTATAAAGCCGAAATGTGTTGGAATTAA
CTTGGCTTATGATACGGATTTAATTATTAGGAAGTACTTAGATGAGAAACCATTTTCTTCGGATATTAAACTTTCATGCCAGTGGACTCGAAGTTTATTCTTGATACCTTGATTTCAACAGCCGATATGTAGAACAATTTTCTTATCACCAGTCCACATTTTTATGGAGCTCTAGTTTATTTCAGGCCCTCGAGGGCTCA
CAGTAATGTTTTACAAATGTAAAAGATGCACAGATTTTAACTCTTCTGGTAAGAATAAATTGAACTGTTGCGAGTTTCTAGGTTATTATATATTAGGTTGGAAAGGGATTACTCCATGCTGAGTTGGGAAAGACATTGCTATTGCATGGGAGAGATGTAAAGCCCTACCTCTATTCCAGAGGCTTGCATTGCATTATTGT
GGTTCATGGGGAGTTGGGGTTCATAACTCACAAATCAATCAAAATCACTGATAATGGTGTTTGACTAGTTAAAAGGATGTATTTTAATGCTTCAGCC

>TRINITY_DN36130_c0_g1_i1 from sample A8 / Example unspliced isoform:

CTCCAAAGCAAATTCAGAAGTACTCAATTTTATATCATTTTTATGGGTTTTTATAGCGAATTAGATTTTTATATTTGTGGAGGCTGTTCATCTATGTCACATACTCAGAATCTGTTTACCTATTAATAGGCTATATTATGAATTCAAAAATTTCACTGCAGTGAATTTTATTGTCAGTTGTATGGGTGATTGCAGGCAGA
ATACATGGACTTGAAAACCTTGCATTCCCGTGTAGAAGATGCTGTAAATACCATGATTCGAAGAGATGAAAGTACTGAAAATGGTCAATTTTTGAAACCTTGTGTTGAAGCGGCTCTCTATGTGGGATGTCCACCAAGGAAAGCACCTAGGAGTCAGAGGCATAATTCACGATTCTGTGCAGGCAGCAGCCAACGACTTA
CTACATCCTCTTCTGTCCCTGTGgtaggcaagtgcaatgaatatgaggggtcttcattggttattcaagcaaactcaaaggaagGCATGTTGCTGATGTCGGGTGTGACTACCATGTACTCTTAAATGCTAGCTGGAGCGAAATCAGGCAGTCAGAGTAACTTGTTCTCACATTCAAAGAGAGTGGCTAAAATTGCTGAG
GCCTCCGCTTGTATTCCTGATGAATTCACTTAACTATAAAGCCGAAATGTGTTGGAATTAACTTGGCTTATGATACGGATTTAATTATTAGGAAGTACTTAGATGAGAAACCATTTTCTTCGGATATTAAACTTTCATGCCAGTGGACTCGAAGTTTATTCTTGATACCTTGATTTCAACAGCCGATATGTAGAACAATT
TTCTTATCACCAGTCCACATTTTTATGGAGCTCTAGTTTATTTCAGGCCCTCGAGGGCTCACAGTAATGTTTTACAAATGTAAAAGATGCACAGATTTTAACTCTTCTGGTAAGAATAAATTGAACTGTTGCGAGTTTCTAGGTTATTATATATTAGGTTGGAAAGGGATTACTCCATGCTGAGTTGGGAAAGACATTGC
TATTGCATGGGAGAGATGTAAAGCCCTACCTCTATTCCAGAGGCTTGCATTGCATTATTGTGGTTCATGGGGAGTTGGGGTTCATAACTCACAAATCAATCAAAATCACTGATAATGGTGTTTGACTAGTTAAAAGGATGTATTTTAATGCTTCAGCC

Intron 2 GTACTCAATT ... GTGATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.13	30	0	6.3
A2	23.52	29	0	9
A3	11.22	39	0	5
A4	10.09	44	0	5
A5	59.38	3	0	4.5
A6	13.69	29	0	4.8
A7	18.68	69	2	16
A8	24.48	35	1	11.5
A9	21.3	55	0	15.8
A10	16.98	62	0	12.8

>TRINITY_DN37926_c0_g1_i2 from sample A7 / Example spliced isoform

GCGGAAGTGGAGTAGAGTAGATTTTAATGTCAATGAAATCTCATCATAGTAATATGGCACTGAGTAAGATTTGGTCCACTCTTCCGTAAAAAGATCCTGAGGGGACAAATAATGGAAACATGAAAGAAATTGTCCCACCATGAGAGCAATCGATGTGAAGAGGGGATCAAAAGAAAGATAATGGGGCCGTGGGTTTCATG
GAAGGCTGTACTTCATAATGAAAGGAAGCTCCTCCACATGAAGTCTGATTGCATGGCATGTCATGTCATTTGGATGTGATTTTGGAGAGAGAGAGAGACGGGGAACAATGTGCAAAAGCTTTGCATAGCATAAGGTTTTGGGCCCGAAAGCAAATTGAGCCCACGAAAGCCGCCCTATTTTTTCAATCTTTGTTCATTTG
GGTCTCTTCCAATAGCGCTTCTGACAAAACCCAATTGACATTTTCTGTCGATAATCTGGACGAAGTACAGAGCTAGTAATGGGTCTCTGGAACGCTACAGCTCATATAGAGGACTCCAAGCTCTGGTAGGATTATTGGGCAGCTTCATATTGAGTTTCTTGGTGCAATTGGGCCGTTGCAATCAGGGAGCCGTGATTATA
GAGAATGCCAAGGCCAGGACCCAGGCCCTATGAGTGTATCAGGAGAGCGTGGCATAGTGACAGCCACCATCCCCTGAGGGGCAGTCTGATACAGGAGATATTCAGGGTTGCAGACGAGGTTCATAGCCCAACTACAAGACAGAATAAAGAATGGCAAAAGAAGCTACCTTTGGTGGTCCTGCGTGCAGAGGAGATATTAT
ACTCCAAAGCAAATTCAGAAGCAGAATACATGGACTTGAAAACCTTGCATTCCCGTGTAGAAGATGCTGTAAATACCATGATTCGAAGAGATGAAAGTACTGAAAATGGTCAATTTTTGAAACCTTGTGTTGAAGCGGCTCTCTATGTGGGATGTCCACCAAGGAAAGCACCTAGGAGTCAGAGGCATAATTCACGATTC
TGTGCAGGCAGCAGCCAACGACTTACTACATCCTCTTCTGTCCCTGTGGTAGGCAAGTGCAATGAATATGAGGGGTCTTCATTGGTTATTCAAGCAAACTCAAAGGAAGGTAGATTTAGTAATTCAGAGTATTGTGCTGGAGTTGCATCGTCTTTCCCGTCAATAGACCACCAACAGTATTCTACAGTGCCTTTCCGTGC
TATACCTAGCTGTCTGAGTTCGGTAGTATGTACACCTAGAATTGACAGCACAACCTCCACTAGAGATACAACCTTTTGTTTTCCTAATCCATGTTCAACAGCCAACATAACTCCTTTAGGTTTTAACCAAGGAATTCCTTCGGCATGTAATGGACCATCTAACCCACAGAAGTTTTTAAGTACGAGCCAAAATGGAATGA
TAGTGCACCAACCCAACCCCCAAACAAGGACAGAGACAAGACTTATTTTGCCTGTACCATGGCCGATGTCTTCAGCATCTACAGGCAATTCACTATCAACCCCCTGGGCAGTTCCAACTTCATTTCAACCAGAAGTGTCAAATGAGTCTTCTATTGGTTTCTATTATCCAGAAAGATTAAGTACTCGTGTACCCGAGACC
CCTGCAGCCCATGTAGATTCTGAAATGCGATTTCAATTGCCTTCAAACAATAGCTATCGCTCAGGGTTCAATCATGGGAAATACATGGCAGAACTGGGCTCTTCGGATGAATGCAATTATAACACTGATGCGTCTTGCAAAAGTTTTCAAAATAGTTCAATGAAAGTAATAAGAGATTTATCTGTAGAGACGGATGGCAA
AACAGCATCTGAGAATGCATGTGATTTACTTTTACGGTTGGGTCCATCTTCAAATCCATGCTCTAATACGGCGACAAATGGAAACTGTGATGTTGAAGACTTAGAATCAAGTAATTCCAGAGCAAGAGTTCAAGTTGAAGATCGGGCTGTAGAAACATTAAAGATCAATGATTCCTTTGGTTCTGGTTCTTGTGAATCAA
ATCATGAATTTCTTTGCTTTTCTCCATCTCTCAATTCTGGTGTCGATGTCCCAGAGGCATTTCCAAAGGAAAAGGGTCCAAATGTTGACATGTATACAGTCAGGCAAGCAGCAGGTGCTGAAATGCAAAGTAGAAAGCGCAGAAAATATGATGATGGATTAACAGTCAATGCATTTGATGGCAAGTCCACAGATCAGCAA
CATGACATGTGTGATCTGCAGATGGGGCTGGGGCTTTCTTTCTCTAAACGAAACTGTGATGTATACAGTATGATGGAATAGTACCTTGTTAGAAACTGGACAGTTGAAGGGATGAAATCTTTTTCTGTTATTGTAAACAATGCCAATTTTGGGCTCAATGCCTGTTGGCTTAAGAGTTCCATTTTCTATAACTGGATCTC
ATAGTAGAAAATGCATTATCATGTATTTTGAAAGCTTCCCTTTTGTTGTTAAGAAATATTCCCACCCAAGTAATTGTATCTTTAGTTGTGAATTGTTATAGGAAAGTGCCCACAGCTAAACATACATATTCAAATTAATCTGTTTTATGAAGTATGGTACTTGAAAATGTCCGTTGCTTGTACCTGCTGAAGCTTTTATG
TATACATCCGTGTTTTACAATTTTGAAAAAAA

>TRINITY_DN37926_c0_g1_i1 from sample A7 / Example unspliced isoform:

ATACTCCAAAGCAAATTCAGAAgtactcaattttatatcatttttatgggtttttatagcgaattagatttttatatttgaggaggctgttcatctatgtcacatactcagaatctgtttacctattaataggctatattatgaattcaaaaatttcactgcagtgaattttattgtcagttgtatgggtgattgcagGC
AGAATACATGGACTTGAAAACCTTGCATTCCCGTGTAGAAGATGCTGTAAATACCATGATTCGAAGAGATGAAAGTACTGAAAATGGTCAATTTTTGAAACCTTGTGTTGAAGGTATGTCATGCTATGTACAATCCTCTGTGA