sp|Q8W463|RK191_ARATH ----> Large ribosomal subunit protein bL19cz

Intron 1 GTAAGTTGCA ... GAATCTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.56	8	8	12
A2	60	11	21	12
A3	64.84	9.5	18	17
A4	72.73	3	2	14
A5	100	0	0	3
A6	65.13	26.5	38	61
A7	76.36	11.5	27	47
A8	64.66	17.5	22	42
A9	68.46	23.5	44	58
A10	79.47	9	28	41

>TRINITY_DN113510_c3_g1_i2 from sample A2 / Example spliced isoform

GGCTTTTAACATGCTTAGCTACAACTTTTAGTTTTATGATGCTAGATTGTTTGTCCCTGGATTGATATTATTATTATTAATATATCAAAGAGTTCAACACTGGAGATGTATATCTACTCATAGGATAAGGTTGGTGTGGTTGATTGTCCATTATTCGTCTCACTACAGAAGAGAAACGAGTTGCACCGTTGCATCCAATG
TTGGCGTGTTGAAGAGAGATATACAGATTCTTGGTTTACTAGCAGTCTTCTATACTGCTGGGGGAGCTCAATGGCAGCAAACATACTTCATCAGGTTCCAGGATTTATGCCCGGCAGAGGTGCTTTGTTTTCTTCAAAATCGGAACTAGCTCTTTCTTTCTCATTTTCATCATCTCTGAATTTAAAAGGACGACCCTTGA
AGACATGTGCACCTTTAACTGCGGCTGCTAAGGTGGCACACCGAATGAAGTAGCTTCTTTGGATGAATCAACTTTGGAAGTATCTGAGGAACAACCTATACTCCCTCCAAGAAAACCAAGGATCAAACTTGGCCAAATCATGGGGATACTACACAAGCAAGCTATTGAAGCCACCGAGAAGGAAAGACCTACTCCTGATG
TCAGAACGGGAGATGTTGTAGAACTTCGAGTGTATGTGCCCGAGACTCGACGCAGATTTTCTGTTTATAAGGGTATAGTGATATCGAGGCAAAATTCTGGTATACATACAACTATTCGGATTCGAAGAATAATTGCTGGTGTTGGCATCGAGATAGTCTTCCCATTATACTCGCCAAACCTCAAAGAAATTAAGGTTGTG
AAACACAGGAAGGTCCGCAGAGCAAGACTGTATTACCTAAGAGAAAAACTCCCGAGGTTTTCAACCTTTAAATGAGGATTCTA

>TRINITY_DN113510_c3_g1_i4 from sample A2 / Example unspliced isoform:

GGCTTTTAACATGCTTAGCTACAACTTTTAGTTTTATGATGCTAGATTGTTTGTCCCTGGATTGATATTATTATTATTAATATATCAAAGAGTTCAACACTGGAGATGTATATCTACTCATAGGATAAGGTTGGTGTGGTTGATTGTCCATTATTCGTCTCACTACAGAAGAGAAACGAGTTGCACCGTTGCATCCAATG
TTGGCGTGTTGAAGAGAGATATACAGATTCTTGGTTTACTAGCAGTCTTCTATACTGCTGGGGGAGCTCAATGGCAGCAAACATACTTCATCAGGTTCCAGGATTTATGCCCGGCAGAGGTGCTTTGTTTTCTTCAAAATCGGAACTAGCTCTTTCTTTCTCATTTTCATCATCTCTGAATTTAAAAGGACGACCCTTGA
AGACATGTGCACCTTTAACTGCGGCTGCTgtaagttgcaactggtgcatgtcatcttcttccactaccagagagagcaattttattgtttatgcacaatctgttgactcggtacctgaatctgaagAAGGTGGCACACCGAATGAAGTAGCTTCTTTGGATGAATCAACTTTGGAAGTATCTGAGGAACAACCTATACTC
CCTCCAAGAAAACCAAGGATCAAACTTGGCCAAATCATGGGGATACTACACAAGCAAGCTATTGAAGCCACCGAGAAGGAAAGACCTACTCCTGATGTCAGAACGGGAGATGTTGTAGAACTTCGAGTGTATGTGCCCGAGACTCGACGCAGATTTTCTGTTTATAAGGGTATAGTGATATCGAGGCAAAATTCTGGTAT
ACATACAACTATTCGGATTCGAAGAATAATTGCTGGTGTTGGCATCGAGATAGTCTTCCCATTATACTCGCCAAACCTCAAAGAAATTAAGGTTGTGAAACACAGGAAGGTCCGCAGAGCAAGACTGTATTACCTAAGAGAAAAACTCCCGAGGTTTTCAACCTTTAAATGAGGATTCTATGACTTTAAAATCTGAGTCA
ACTCATTGTTCATGATTGCTAATTGCTTATTTTGGTAAGTAAATAATTGACCAATGCAGTCCAATATCTGTAAAATGAAGGTGTCAAATGTGGGCAATGTTGGCTGCCATATGTTATAAGAAATTCTGGGACATCATTATTTGATGTTGAAAAGTTTTTTTGATTCCAACCCATACAGGTGACCAACAAATTGCATGAAT
TTCTTTACATCTTATTTTTGTATGTTACTCTCTATTTGATGAATGACATGATATTTTTCTTGATTGGTCTCATGCAAGTTACCCAAT

Intron 2 GTAGAATGTC ... AGAAATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.82	15	0	7
A2	29.03	22	0	9
A3	29.03	22	4	9
A4	33.33	12	0	6
A5	36	16	0	9
A6	31.09	82	14	37
A7	37.93	54	4	33
A8	30.43	32	2	14
A9	34.29	69	7	36
A10	35.42	31	5	17

>TRINITY_DN41324_c1_g1_i2 from sample A9 / Example spliced isoform

CTTAGTTCCAAGATTATGTGACGAGGGCTTTTAACATGCTTAGCTACAACTTTTAGTTTTATGATGCTAGATTGTTTGTCCCTGGATTGATATTAGTATTATTAATATATCAAAGAGTTCAACACTGGAGATGTATATCTACTCATAGGATAAGGTTGGTGTGGTTGATTGTCCATTATTCATCTCACTACAGAAGAGAA
ACGAGTTGCACCGTTGCATCCAATGTTGGCGTGTTGAAGAGAGATATACAGATTCTTGGTTTACTAGCAGTCTTCTATACTGCTGGGGGAGCTCAATGGCAGCAAACATACTTCATCAGGTTCCAGGATTTATGCCCGGCAGAGGTGCTTTGTTTTCTTCAAAATCGGAACTAGCTCTTTCTTTCTCATTTTCATCATCT
CTGAATTTAAAAGGACGACCCTTGAAGACATGTGCACCTTTAACTGCGGCTGCTGTAAGTTGCAACTGGTGCATGTCATCTTCTTCCACTACCAGAGAGAGCAATTTTATTGTTTATGCACAATCTGTTGACTCGGTACCTGAATCTGAAGAAGGTGGCACACCGAATGAAGTAGCTTCTTTGGATGAATCAACTTTGGA
AGTATCTGAGGAACAACCTATACTCCCTCCAAGAAAACCAAGGATCAAACTTGGCCAAATCATGGGGATACTACACAAGCAAGCTATTGAAGCCACCGAGAAGGAAAGACCTACTCCTGATGTCAGAACGGGAGATGTTGTAGAACTTCGAGTGTATGTGCCCGAGACTCGACGCAGATTTTCTGTTTATAAGGGTATAG
TGATATCGAGGCAAAATTCTGGTATACATACAACTATTCGGATTCGAAGAATAATTGCTGGCGTTGGCATCGAGATAGTCTTCCCATTATACTCGCCAAACCTCAAAGAAATTAAGGTTGTGAAACACAGGAAGGTCCGCAGAGCAAGACTGTATTACCTAAGAGAAAAACTCCCGAGGTTTTCAACCTTTAAATGAGGA
TTCTAGGACTTTAAAATCTGAGTCGGCTCATTGTTCATGATTGCTAATTGCTTATTTTGGTAAGTAAATAATTGACCAATGCAGTCCAATATCTGTAAAATGAAGGTGTCAAATGTGGGCAATGTTGGCTGCCATATGTTATAAGAAATTCTGGGACATCATTATTTGATGTTGAAAAGTTTTTTTGATTCCAACCCATA
CAGGTGACCAACAAATTGCATGAATTTCTTTACATCTTATTTTTGTATGTTACTCTCTATTTGATGAATGACATGATATTTTTCTTGATTGGTCTCATGCAAGTTACCCAATATAGCTAGTCACTCGTATCAATGTGGAACAAAATCCTTCAATTTTAGTAGCGTTGAGGAAGAGGTG

>TRINITY_DN41324_c1_g1_i1 from sample A9 / Example unspliced isoform:

CTTAGTTCCAAGATTATGTGACGAGGGCTTTTAACATGCTTAGCTACAACTTTTAGTTTTATGATGCTAGATTGTTTGTCCCTGGATTGATATTAGTATTATTAATATATCAAAGAGTTCAACACTGGAGATGTATATCTACTCATAGGATAAGGTTGGTGTGGTTGATTGTCCATTATTCATCTCACTACAGAAGAGAA
ACGAGTTGCACCGTTGCATCCAATGTTGGCGTGTTGAAGAGAGATATACAGgtagaatgtcactggattttcacaactgaattgagaagaggaaggagaagatctatttggcgtttttgagctttggacgaactcttccaaccatcccggatcaagaaatacagATTCTTGGTTTACTAGCAGTCTTCTATACTGCTGGG
GGAGCTCAATGGCAGCAAACATACTTCATCAGGTTCCAGGATTTATGCCCGGCAGAGGTGCTTTGTTTTCTTCAAAATCGGAACTAGCTCTTTCTTTCTCATTTTCATCATCTCTGAATTTAAAAGGACGACCCTTGAAGACATGTGCACCTTTAACTGCGGCTGCTGTAAGTTGCAACTGGTGCATGTCATCTTCTTCC
ACTACCAGAGAGAGCAATTTTATTGTTTATGCACAATCTGTTGACTCGGTACCTGAATCTGAAGAAGGTGGCACACCGAATGAAGTAGCTTCTTTGGATGAATCAACTTTGGAAGTATCTGAGGAACAACCTATACTCCCTCCAAGAAAACCAAGGATCAAACTTGGCCAAATCATGGGGATACTACACAAGCAAGCTAT
TGAAGCCACCGAGAAGGAAAGACCTACTCCTGATGTCAGAACGGGAGATGTTGTAGAACTTCGAGTGTATGTGCCCGAGACTCGACGCAGATTTTCTGTTTATAAGGGTATAGTGATATCGAGGCAAAATTCTGGTATACATACAACTATTCGGATTCGAAGAATAATTGCTGGCGTTGGCATCGAGATAGTCTTCCCAT
TATACTCGCCAAACCTCAAAGAAATTAAGGTTGTGAAACACAGGAAGGTCCGCAGAGCAAGACTGTATTACCTAAGAGAAAAACTCCCGAGGTTTTCAACCTTTAAATGAGGATTCTAGGACTTTAAAATCTGAGTCGGCTCATTGTTCATGATTGCTAATTGCTTATTTTGGTAAGTAAATAATTGACCAATGCAGTCC
AATATCTGTAAAATGAAGGTGTCAAATGTGGGCAATGTTGGCTGCCATATGTTATAAGAAATTCTGGGACATCATTATTTGATGTTGAAAAGTTTTTTTGATTCCAACCCATACAGGTGACCAACAAATTGCATGAATTTCTTTACATCTTATTTTTGTATGTTACTCTCTATTTGATGAATGACATGATATTTTTCTTG
ATTGGTCTCATGCAAGTTACCCAATATAGCTAGTCACTCGTATCAATGTGGAACAAAATCCTTCAATTTTAGTAGCGTTGAGGAAGAGGTG

Intron 3 GTTCCAGGAT ... AATTTAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	94.74	1	18	18
A2	69.23	12	26	28
A3	69.86	11	28	23
A4	64.1	7	17	8
A5	75	2	11	1
A6	71.89	26	79	54
A7	76.12	16	63	39
A8	78.82	9	36	31
A9	71.43	27	84	51
A10	76.47	10	37	28

>TRINITY_DN106748_c0_g1_i6 from sample A3 / Example spliced isoform

CTTAGTTCCAAGATTATGTGACGAGGGCTTTTAACATGCTTAGCTACAACTTTTAGTTTTATGATGCTAGATTGTTTGTCCCTGGATTGATATTAGTATTATTAATATATCAAAGAGTTCAACACTGGAGATGTATATCTACTCATAGGATAAGGTTGTTGTGGTTGATTGTCCATTATTCGTCTCACTACAGAAGAGAA
ACGAGTTGCACCGTTGCATCCAATGTTGGCGTGTTGAAGAGAGATATACAGATTCTTGGTTTACTAGCAGTCTTCTATACTGCTGGGGGAGCTCAATGGCAGCAAACATACTTCATCAGGACGACCCTTGAAGACATGTGCACCTTTAACTGCGGCTGCTGTAAGTTGCAACTGGTGCATGTCATCTTCTTCCACTACCA
GAGAGAGCAATTTTATTGTTTATGCACAATCTGTTGACTCGGTACCTGAATCTGAAGAAGGTGGCACACAGAATGAAGTAGCTTCTTTGGATGAATCAACTTTGGAAGTATCTGAGGATCAACCTATACTCCCTCCAAGAAAACCAAGGATCAAACTTGGCCAAATCATGGGGATACTACACAAGCAAGCTATTGAAGCC
ACCGAGAAGGAAAGACCTACTCCTGATGTCAGAACGGGAGATGTTGTAGAACTTCGAGTGTATGTGCCCGAGACTCGACGCAGATTTTCTGTTTATAAGGGTATAGTGATATCGAGGCAAAATTCTGGTATACATACAACTATTCGGATTCGAAGAATAATTGCTGGCGTTGGCATCGAGATAGTCTTCCCATTATACTC
GCCAAACCTCAAAGAAATTAAGGTTGTGAAACACAGGAAGGTCCGCAGAGCAAGACTGTATTACCTAAGAGAAAAACTCCCGAGGTTTTCAACCTTTAAATGAGGATTCTAGGACTTTAAAATCTGAGTCGGCTCATTGTTCATGATTGCTAATTGCTTATTTTGGTAAGTAAATAATTAACCAATGCAGTCCAATATCT
GTAAAATGAAGGTGGCAAATGTGGGCAATGTTGGCTGCCATATGTTATAAGAAATTCTGGGACATCATTATTTGATGTTGAAAAGTTTTTTTGATTCCAACCCATACAGGTGACCAACAAATTGCATGAATTTCTTTACATCTTATTTTTGTATGTTACTCTCTATTTGATGAATG

>TRINITY_DN106748_c0_g1_i3 from sample A3 / Example unspliced isoform:

CTTAGTTCCAAGATTATGTGACGAGGGCTTTTAACATGCTTAGCTACAACTTTTAGTTTTATGATGCTAGATTGTTTGTCCCTGGATTGATATTAGTATTATTAATATATCAAAGAGTTCAACACTGGAGATGTATATCTACTCATAGGATAAGGTTGTTGTGGTTGATTGTCCATTATTCGTCTCACTACAGAAGAGAA
ACGAGTTGCACCGTTGCATCCAATGTTGGCGTGTTGAAGAGAGATATACAGATTCTTGGTTTACTAGCAGTCTTCTATACTGCTGGGGGAGCTCAATGGCAGCAAACATACTTCATCAGgttccaggatttatgcctggcagaggtgctttgctttcttcaaaatcagaactagctctttctttctcattttcatcatct
ctgaatttaaaagGACGACCCTTGAAGACATGTGCACCTTTAACTGCGGCTGCTGTAAGTTGCAACTGGTGCATGTCATCTTCTTCCACTACCAGAGAGAGCAATTTTATTGTTTATGCACAATCTGTTGACTCGGTACCTGAATCTGAAGAAGGTGGCACACAGAATGAAGTAGCTTCTTTGGATGAATCAACTTTGGA
AGTATCTGAGGATCAACCTATACTCCCTCCAAGAAAACCAAGGATCAAACTTGGCCAAATCATGGGGATACTACACAAGCAAGCTATTGAAGCCACCGAGAAGGAAAGACCTACTCCTGATGTCAGAACGGGAGATGTTGTAGAACTTCGAGTGTATGTGCCCGAGACTCGACGCAGATTTTCTGTTTATAAGGGTATAG
TGATATCGAGGCAAAATTCTGGTATACATACAACTATTCGGATTCGAAGAATAATTGCTGGCGTTGGCATCGAGATAGTCTTCCCATTATACTCGCCAAACCTCAAAGAAATTAAGGTTGTGAAACACAGGAAGGTCCGCAGAGCAAGACTGTATTACCTAAGAGAAAAACTCCCGAGGTTTTCAACCTTTAAATGAGGA
TTCTAGGACTTTAAAATCTGAGTCGGCTCATTGTTCATGATTGCTAATTGCTTATTTTGGTAAGTAAATAATTAACCAATGCAGTCCAATATCTGTAAAATGAAGGTGGCAAATGTGGGCAATGTTGGCTGCCATATGTTATAAGAAATTCTGGGACATCATTATTTGATGTTGAAAAGTTTTTTTGATTCCAACCCATA
CAGGTGACCAACAAATTGCATGAATTTCTTTACATCTTATTTTTGTATGTTACTCTCTATTTGATGAATG

Intron 4 GTAAGTTGCA ... TGGCACACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	56.1	9	8	15
A2	58.33	10	21	7
A3	67.35	8	18	15
A4	72.73	3	2	14
A5	100	0	0	3
A6	60.45	35	38	69
A7	76.47	12	27	51
A8	61.47	21	22	45
A9	65.38	27	44	58
A10	81.18	8	28	41

>TRINITY_DN39820_c0_g2_i2 from sample A6 / Example spliced isoform

GTGACGAGGGCTTTTAACATGCTTAGCTACAACTTTTAGTTTTATGATGCTAGATTGTTTGTCCCTGGATTGATATTAGTATTATTAATATATCAAAGAGTTCAACACTGGAGATGTATATCTACTCATAGGATAAGGTTGGTGTGGTTGATTGTCCATTATTCATCTCACTACAGAAGAGAAACGAGTTGCACCGTTGC
ATCCAATGTTGGCGTGTTGAAGAGAGATATACAGGAAATACAGATTCTTGGTTTACTAGCAGTCTTCTATACTGCTGGGGGAGCTCAATGGCAGCAAACATACTTCATCAGGTTCCAGGATTTATGCCTGGCAGAGGTGCTTTGCTTTCTTCAAAATCAGAACTAGCTCTTTCTTTCTCATTTTCATCATCTCTGAATTT
AAAAGGACGACCCTTGAAGACATGTGCACCTTTAACTGCGGCTGCTAATGAAGTAGCTTCTTTGGATGAATCAACTTTGGAAGTATCTGAGGATCAACCTATACTCCCTCCAAGAAAACCAAGGATCAAACTTGGCCAAATCATGGGGATACTACACAAGCAAGCTATTGAAGCCACCGAGAAGGAAAGACCTACTCCTG
ATGTCAGAACGGGAGATGTTGTAGAACTTCGAGTGTATGTGCCCGAGACTCGACGCAGATTTTCTGTTTATAAGGGTATAGTGATATCGAGGCAAAATTCTGGTATACATACAACTATTCGGATTCGAAGAATAATTGCTGGTGTTGGCATCGAGATAGTCTTCCCATTATACTCGCCAAACCTCAAAGAAATTAAGGTT
GTGAAACACAGGAAGGTCCGCAGAGCAAGACTGTATTACCTAAGAGAAAAACTCCCGAGGTTTTCAACCTTTAAATGAGGATTCTATGACTTTAAAATCTGAGTCAACTCATTGTTCATGATTGCTAATTGCTTATTTTGGTAAGTAAATAATTGACCAATGCAGTCCAATATCTGTAAAATGAAGGTGTCAAATGTGGG
CAATGTTGGCTGCCATATGTTATAAGAAATTCTGGGACATCATTATTTGATGTTGAAAAGTTTTTTTGATTCCAACCCATACAGGTGACCAACAAATTGCATGAATTTCTTTACATCTTATTTTTGTATGTTACTCTCTATTTGATGAATGACATGATATTTTTCTTGATTGGTCTCATGCAAGTTA

>TRINITY_DN39820_c0_g2_i1 from sample A6 / Example unspliced isoform:

GTGACGAGGGCTTTTAACATGCTTAGCTACAACTTTTAGTTTTATGATGCTAGATTGTTTGTCCCTGGATTGATATTAGTATTATTAATATATCAAAGAGTTCAACACTGGAGATGTATATCTACTCATAGGATAAGGTTGGTGTGGTTGATTGTCCATTATTCATCTCACTACAGAAGAGAAACGAGTTGCACCGTTGC
ATCCAATGTTGGCGTGTTGAAGAGAGATATACAGGAAATACAGATTCTTGGTTTACTAGCAGTCTTCTATACTGCTGGGGGAGCTCAATGGCAGCAAACATACTTCATCAGGTTCCAGGATTTATGCCTGGCAGAGGTGCTTTGCTTTCTTCAAAATCAGAACTAGCTCTTTCTTTCTCATTTTCATCATCTCTGAATTT
AAAAGGACGACCCTTGAAGACATGTGCACCTTTAACTGCGGCTGCTgtaagttgcaactggtgcatgtcatcttcttccactaccagagagagcaattttattgtttatgcacaatctgttgactcggtacctgaatctgaagaaggtggcacacagAATGAAGTAGCTTCTTTGGATGAATCAACTTTGGAAGTATCTG
AGGATCAACCTATACTCCCTCCAAGAAAACCAAGGATCAAACTTGGCCAAATCATGGGGATACTACACAAGCAAGCTATTGAAGCCACCGAGAAGGAAAGACCTACTCCTGATGTCAGAACGGGAGATGTTGTAGAACTTCGAGTGTATGTGCCCGAGACTCGACGCAGATTTTCTGTTTATAAGGGTATAGTGATATCG
AGGCAAAATTCTGGTATACATACAACTATTCGGATTCGAAGAATAATTGCTGGTGTTGGCATCGAGATAGTCTTCCCATTATACTCGCCAAACCTCAAAGAAATTAAGGTTGTGAAACACAGGAAGGTCCGCAGAGCAAGACTGTATTACCTAAGAGAAAAACTCCCGAGGTTTTCAACCTTTAAATGAGGATTCTATGA
CTTTAAAATCTGAGTCAACTCATTGTTCATGATTGCTAATTGCTTATTTTGGTAAGTAAATAATTGACCAATGCAGTCCAATATCTGTAAAATGAAGGTGTCAAATGTGGGCAATGTTGGCTGCCATATGTTATAAGAAATTCTGGGACATCATTATTTGATGTTGAAAAGTTTTTTTGATTCCAACCCATACAGGTGAC
CAACAAATTGCATGAATTTCTTTACATCTTATTTTTGTATGTTACTCTCTATTTGATGAATGACATGATATTTTTCTTGATTGGTCTCATGCAAGTTA