sp|Q40864|EML_PICGL ----> Em-like protein

Intron 1 GTACTGATTG ... TAAATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.33	708	192	9
A2	22.3	958	275	8
A3	21.35	1131	307	8
A4	23.78	737	230	4
A5	19.56	732	178	2
A6	23.16	14957	4508	49
A7	23.4	10198	3115	44
A8	21.92	8024	2252	33
A9	22.68	7432	2180	24
A10	22.13	8059	2290	21

>TRINITY_DN40203_c0_g5_i2 from sample A6 / Example spliced isoform

AGTTAATGTTACTGTTTGGTATTTCTTGTTGAAGTTACTGCGTTTTCTTTTGTTTATTCAGAGAAGAGTGATTGTTATAAGTAAAGATGGCACATAGGCCAGTTACGAGAGAGCTGGATGCCAAGGCGTGGGAGGGAGAGACTATCGTGCCCGGTGGCACCGGTGGGAAGTCCGTTGATGCCCAAGAGCGTCTTGCTGAA
GGGCGGAGCAGAGGTGGCCAGACTCGAAAGGAACAAATTGGCAGCGAAGGATACCAGGAGATGGGCCGCAAGGGAGGGCTTACCACACAAGACAAGTCCGGTGCAGAACGGGCAGCTGAAGAGGGTATCCCCATTGATGAGTCCAAATACATGACCAATTGATTCCAGCTGTTACCGTTTTCCTGCAATATATATACACA
TGGATATATATTGTATATATCCTCTGTGTTCCAACTTCCGCCAACAGATTTCTTCTTCTTTCGAAATTTCTCTATTGTCATATAGTTATCGTTTCTTTGTGTTACATGTTAGTTTGATTTCAATAACCATTCGTTGGTTTCCTCTCCTTTTTTAATGAAATAAATCTTCTTTGTTATAAACACTGGAATGACTCTGCCTA
TTAAGTTTTGATGGAGG

>TRINITY_DN40203_c0_g5_i4 from sample A6 / Example unspliced isoform:

AGTTAATGTTACTGTTTGGTATTTCTTGTTGAAGTTACTGCGTTTTCTTTTGTTTATTCAGAGAAGAGTGATTGTTATAAGTAAAGATGGCACATAGGCCAGTTACGAGAGAGCTGGATGCCAAGGCGTGGGAGGGAGAGACTATCGTGCCCGGTGGCACCGGTGGGAAGTCCGTTGATGCCCAAGAGCGTCTTGCTGAA
GgtactgattggcttgtattataaatatatatttcactgtattctagtaatataatatagttcttgacggttttcattgaattaaattgcagGGCGGAGCAGAGGTGGCCAGACTCGAAAGGAACAAATTGGCAGCGAAGGATACCAGGAGATGGGCCGCAAGGGAGG

Intron 2 GTATGATTGC ... TGAATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	7.07	698	24	53.2
A2	6.02	947	30.4	60.8
A3	7.36	1126	27.2	89.6
A4	6.55	722	34	50.6
A5	4.84	735	16	37.4
A6	7.11	14812	267	1137
A7	6.12	10089	154.2	659.4
A8	6.99	7986	103.2	602.4
A9	6.61	7368	102.4	523
A10	5.78	7966	126	490.2

>TRINITY_DN107228_c0_g1_i2 from sample A2 / Example spliced isoform

CTTGAAGTTAAAGTAATTACTATTACTGTTTGGTATTTCTACTGATCTCAAGAAGGCGATACTGCGTTTTGTTTTGCTGTGCTTTTTTTTGTTGATTCAGAGAAAGCTGTAGTGAGTATTATAAGAAGGAAAGATGGCATATAGGCAAGATCCCAGAGAGCTGGATGCCAAGGCGCGGGAGGGCGAGACTGTGGTGCCCG
GTGGCACCGGTGGGAAGTCCGTTGATGCCCAAGAGCGTCTTGCTGAAGGGCGTAGCAGAGGCGGCCAAACTCGAAAGGAACAAATTGGTAGCGAAGGATACCAGGAGATGGGCCGCAAGGGAGGTCTCACCACACAAGACAAGCCCGGTGGAGAACGGGCAGCTGAAGAGGGTATCCCCATTGACGAGTCCAAATACAAG
AACACTTGATTCCTGTTAGCGTTGTCACAATATATATACATAGATATATATATTGTATATACCCTGATCAGTGTTTCAACTTCCGCCATCATATTTCTTCTTCCGATTTGTCTATTGTAATATAGTTATCGTTTACTTTGTGGTGTACGTTGGTGTATGTCAATAACCATTCGTTGGTTTCTTCTCCTGTGAATTTAATG
AAATAAACCTTCTTTGTATATTTCATGAAATACAACTTCTTTGTTATATATACAGTGTACACTGTACTGCACTGA

>TRINITY_DN107228_c0_g1_i5 from sample A2 / Example unspliced isoform:

CCAAGAGCGTCTTGCTGAAGgtatgattgccttgtattataaatacatatttcaatattgtattcattataattcttgacggttttcattgaattgaattgcagGGCGTAGCAGAGGCGGCCAAACTCGAAAGGAACAAATTGGTAGCGAAGGATACCAGGAGATGGGCCGCAAGGGAGGTCTCACCACACAAGACAAGC
CCGGTGGAGAACGGGCAGCTGAAGAGGGTATCCCCATTGACGAGTCCAAATACAAGAACACTTGATTCCTGTTAGCGTTGTCACAATATATATACATAGATATATATATTGTATATACCCTGATCAGTGTTTCAACTTCCGCCATCATATTTCTTCTTCCGATTTGTCTATTGTAATATAGTTATCGTTTACTTTGTGGT
GTACGTTGGTGTATGTCAATAACCATTCGTTGGTTTCTTCTCCTGTGAATTTAATGAAATAAACCTTCTTTGTATATTTCATGAAATACAACTTCTTTGTTATATATACAGTGTACACTGTACTGCACTGA