sp|P46086|KIME_ARATH ----> Mevalonate kinase

Intron 1 GCATGGTGAA ... CTGCAACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.56	4	7	3
A2	73.59	4.5	10	15
A3	56.09	5.5	7	7
A4	42.86	10	11	4
A5	89.47	1	8	7
A6	61.84	25	37	44
A7	45.63	18	26	4
A8	47.16	18.5	18	15
A9	47.34	12.5	16	6
A10	41.43	17	22	2

>TRINITY_DN40850_c0_g1_i7 from sample A8 / Example spliced isoform

GAAGGGCAGTGGCGACTGCACGATATCCCTAAGGCGCAGCAGCAGGTTGCGGTAGAAAACTTGGGCAATCAATTTGTTGTTGGTAGATTTGAGGTTTTGCATAGAACAACCGCCATTAACAACAAACCGCCTTATGTTTTGTATCGGAACCTGTTACTGTTTCTTAGGGTTCAAGCAACTACGGAAACCCCTCGCGACTG
CTGTGTGATACGCCTAATGCGCAGCATTAGGTTCTGATAGAAGACTCCGGCTATGCGTTTCTGATAGAAGACTCCGGCTATGCGTTTGTTTGTTGGTGGGTTGAATGCTTGAAGCAGAGCCACCAACAACAAACGGCCTGGGGTTTTGTATCGGAATCTGTTACCGTTTCCTACGCGTCAAGCAACAATCTAAAATCTCC
TCGCAAAATCTGAAATGGCTGTGGAATATCGTTCTGGAGTCGGATCTTCGCCGAACCCACTTGGGGCCCTAAAACTCTCACTTCCGCCTATGAAATAGCGCTTCTCTCCCAACTCTAAAAACATCAATATCTAACATGCCCGATCTCCAGGTTGAAGCCTGCCACAGTTATTGTGATTTCAGACACACTTCTAGCCTGTG
GGTTCATGTTTGAGATCATGTGCTGCCTTTTGCATGGCAGTATTGGGCGCTGTCCTTGCCTTATTGGACACACTAGATTGGGATGCTATCTAAGGGAATTGATTGAGTTTGAAACAAAGTGATTGCAAGATAGTGTACATGTGGGTTTCCAAAGGAGGAAAAATCATCCACGGTGGACCTTCAGACATTGACAATGCGAT
TAGTGCACATTGTATGTAAATAAAATTTGCTATAAAGACGGATAATGAAACATGATTGCTTTAGTCGTTATTGGTGGTTGTTAACTAGTAACAGCATTTCTTAGCGTTTTTGGAATGCGCTTATAACTGGTTCTTTTATTGTGTGTATACATGTCAGAAATCACTGTAGTTTGCTTCTCTCTGCTGTCATGGCTCAGGAG
TTTGCTTAAAAAATCAAATATCTGAACTTCAAGTTATTT

>TRINITY_DN40850_c0_g1_i2 from sample A8 / Example unspliced isoform:

GAAGGGCAGTGGCGACTGCACGATATCCCTAAGGCGCAGCAGCAGGTTGCGGTAGAAAACTTGGGCAATCAATTTGTTGTTGGTAGATTTGAGGTTTTGCATAGAACAACCGCCATTAACAACAAACCGCCTTATGTTTTGTATCGGAACCTGTTACTGTTTCTTAGGGTTCAAGCAACTACGGAAACCCCTCGCGACTG
CTGTGTGATACGCCTAATGCGCAGCATTAGGTTCTGATAGAAGACTCCGGCTATGCGTTTCTGATAGAAGACTCCGGCTATGCGTTTGTTTGTTGGTGGGTTGAATGCTTGAAGCAGAGCCACCAACAACAAACGGCCTGGGGTTTTGTATCGGAATCTGTTACCGTTTCCTACGCGTCAAGCAACAATCTAAAATCTCC
TCGCAAAATCTGAAATGGCTGTGGAATATCGTTCTGGAGgcatggtgaaattgatccgattaaggccttggccagtttaggcggttagctgtttagcagttctagggtttttatttatttattagtttctctgttaacaatgcttttggtatttcatttatcataaggcttttgtgcaaagttgtgatttctggttctgg
atctgcaaccagTCGGATCTTCGCCGAACCCACTTGGGGCCCTAAAACTCTCACTTCCGCCTATGAAATAGCGCTTCTCTCCCAACTCTAAAAACATCAATATGTGAGTATTTCATAGCAGTCTACTCTTTTTGGACCCTTAAAGCATTTGGAAG

Intron 2 GTTAGCTGTT ... CTGCAACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.43	1	2	3
A2	59.38	9	11	15.3
A3	46	6.3	3	7.7
A4	43.72	3.7	2	3.7
A5	52.26	7	10	5.3
A6	57.03	23.7	18	44.7
A7	23.26	28	11	6
A8	28.24	23.7	6	12.7
A9	30.97	23.7	13	8.3
A10	34.85	14.3	12	3.3

>TRINITY_DN106114_c0_g1_i1 from sample A2 / Example spliced isoform

AGTGGCGACTGCACGATATCCCTAAGGCGCAGCAGCAGGTTGCGGTAGAAAACTTGGGCAATCAATTTGTTGTTGGTAGATTTGAGGTTTTGCATAGAACAACCGCCATTAACAACAAACCGCCTTATGTTTTGTATCGGAACCTGTTACTGTTTCTTAGGGTTCAAGCAACTACGGAAACCCCTCGCGACTGCTGTGTG
ATACGCCTAATGCGCAGCATTAGGTTCTGATAGAAGACTCCGGCTATGCGTTTCTGATAGAAGACTCCGGCTATGCGTTTGTTTGTTGGTGGGTTGAATGCTTGAAGCAGAGCCACCAACAACAAACGGCCTGGGGTTTTGTATCGGAATCTGTTACCGTTTCCTACGCGTCAAGCAACAATCTAAAATCTCCTCGCAAA
ATCTGAAATGGCTGTGGAATATCGTTCTGGAGGCATGGTGAAATTGATCCGATTAAGGCCTTGGCCAGTTTAGGCGTCGGATCTTCGCCGAACCCACTTGGGGCCCTAAAACTCTCACTTCCGCCTATGAAATAGCGCTTCTCTCCCAACTCTAAAAACATCAATATTGCATTGCTACCCTGGTGAATGAACCAAGAGTT
CCCATACTCTGTAATTGGTGTGGTGCTGGAGATTCAGCTTTTTTTCCCCTTATACGTCTCCTTTTTATGTATGCTTTGGTACTACCATTGGACTTGGGTGTTTTCCTTTAAGGACTGCTTGTATTAGGTCTTGGCATCTCAGTTTTGGATCTCTGTAGAATCTCTTTTATTTGTAACAGTATATGTAGATTAACATAAGC
AGCTCCAGGCGTATTAAAATTTTTCGCTGCCATTAAAACTGTTTTTTTGTACAGCTAACATGCCCGATCTCCAGGTTGAAGCCTGCCACAGTTATTGTGATTTCAGACACACTTCTAGCCTGTGGGTTCATGTTTGAGATCATGTGCTGCCTTTTGCATGGCAGTATTGGGCGCTGTCCTTGCCTTATTGGACACACTAG
ATTGGGATGCTATCTAAGGGAATTGATTGAGTTTGAAACAAAGTGATTGCAAGATAGTGTACATGTGGGTTTCCAAAGGAGGAAAAATCATCCACGGTGGACCTTCAGACATTGACAATGCGATTAGTGCACATTGTATGTAAATAAAATTTGCTATAAAGACGGATAATGAAAC

>TRINITY_DN106114_c0_g1_i4 from sample A2 / Example unspliced isoform:

AGTGGCGACTGCACGATATCCCTAAGGCGCAGCAGCAGGTTGCGGTAGAAAACTTGGGCAATCAATTTGTTGTTGGTAGATTTGAGGTTTTGCATAGAACAACCGCCATTAACAACAAACCGCCTTATGTTTTGTATCGGAACCTGTTACTGTTTCTTAGGGTTCAAGCAACTACGGAAACCCCTCGCGACTGCTGTGTG
ATACGCCTAATGCGCAGCATTAGGTTCTGATAGAAGACTCCGGCTATGCGTTTCTGATAGAAGACTCCGGCTATGCGTTTGTTTGTTGGTGGGTTGAATGCTTGAAGCAGAGCCACCAACAACAAACGGCCTGGGGTTTTGTATCGGAATCTGTTACCGTTTCCTACGCGTCAAGCAACAATCTAAAATCTCCTCGCAAA
ATCTGAAATGGCTGTGGAATATCGTTCTGGAGGCATGGTGAAATTGATCCGATTAAGGCCTTGGCCAGTTTAGGCGgttagctgtttagcagttctagggtttttatttatttattagtttctctgttaacaatgcttttggtatttcatttatcataaggcttttgtgcaaagttgtgatttctggttctggatctgca
accagTCGGATCTTCGCCGAACCCACTTGGGGCCCTAAAACTCTCACTTCCGCCTATGAAATAGCGCTTCTCTCCCAACTCTAAAAACATCAATATTGCATTGCTACCCTGGTGAATGAACCAAGAGTTCCCATACTCTGTAATTGGTGTGGTGCTGGAGATTCAGCTTTTTTTCCCCTTATACGTCTCCTTTTTATGTA
TGCTTTGGTACTACCATTGGACTTGGGTGTTTTCCTTTAAGGACTGCTTGTATTAGGTCTTGGCATCTCAGTTTTGGATCTCTGTAGAATCTCTTTTATTTGTAACAGTATATGTAGATTAACATAAGCAGCTCCAGGCGTATTAAAATTTTTCGCTGCCATTAAAACTGTTTTTTTGTACAGCTAACATGCCCGATCTC
CAGGTTGAAGCCTGCCACAGTTATTGTGATTTCAGACACACTTCTAGCCTGTGGGTTCATGTTTGAGATCATGTGCTGCCTTTTGCATGGCAGTATTGGGCGCTGTCCTTGCCTTATTGGACACACTAGATTGGGATGCTATCTAAGGGAATTGATTGAGTTTGAAACAAAGTGATTGCAAGATAGTGTACATGTGGGTT
TCCAAAGGAGGAAAAATCATCCACGGTGGACCTTCAGACATTGACAATGCGATTAGTGCACATTGTATGTAAATAAAATTTGCTATAAAGACGGATAATGAAAC