sp|Q9SXZ2|FT_ARATH ----> Protein FLOWERING LOCUS T

Intron 1 GTAGTTTCCA ... GTCTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.01	53	95	78
A2	57.45	50	76	59
A3	65.17	35	79	52
A4	67.46	48	122	77
A5	71.85	19	49	48
A6	64.9	63	121	112
A7	59.38	156	252	204
A8	63.01	96	172	155
A9	65.13	125	257	210
A10	59.08	142	224	186

>TRINITY_DN48487_c1_g1_i4 from sample A10 / Example spliced isoform

CTTTCAAGAATCCAAGGATTCATTAACCGCAGTTGGTATCGTGTTGAATGTCACTATTTTTGCGATAGAGTTTCTGCTCTTCCGTCTTTCAAGAATCCAAGGATTCATTAACCGCAGTTGGTATCGTGTTGAATGTCACTATTTTTGCGATAGAGTTTCTGCTGTTCCTTCTTTCAAGAATCCAAGGATTCATGAACCGC
AGTCGAAATGCTCCATCAATTTAGTTCTACCAGACAAGATTTGAATACCGCTCGACAATGGAGGAGATAAAGCGAAAACTTGTGAAAGCAGCAGTTATCGGGCGTGTTGTTGACGATTTCAATCCTCATATAAAGTTGAATGTATTCTACGGCGATAAACGAATCAGCCTAGGAACTCAAGTATCTCTGGCTGAAGTGCA
ACACAAACCTGATCATCTTCAATACCGTTACGATCCTGATCATTGCAAGTTCTTGGGAACATTGAAGAACCCTATATTACTAACAGTGGTTATGACGGATCCTGACGCTCCTGGAGGCGAGTATTTGTACTGGTTATCTAGGCATTTCATTGCCGCCCAACAAGAACTAGTGGTGAAATGGCA

>TRINITY_DN48487_c1_g1_i2 from sample A10 / Example unspliced isoform:

CTTTCAAGAATCCAAGGATTCATTAACCGCAGTTGGTATCGTGTTGAATGTCACTATTTTTGCGATAGAGTTTCTGCTCTTCCGTCTTTCAAGAATCCAAGGATTCATTAACCGCAGTTGGTATCGTGTTGAATGTCACTATTTTTGCGATAGAGTTTCTGCTGTTCCTTCTTTCAAGAATCCAAGGATTCATGAACCGC
AGTCGAAATGCTCCATCAATTTAGTTCTACCAGACAAGATTTGAATACCGCTCGgtagtttccaagtgctttcgcaagcaacctttattatttccttcttcttgatttgagacttctttgtgcattatgtctctgcagACAATGGAGGAGATAAAGCGAAAACTTGTGAAAGCAGCAGTTATCGGGCGTGTTGTTGACGA
TTTCAATCCTCATATAAAGTTGAATGTATTCTACGGCGATAAACGAATCAGCCTAGGAACTCAAGTATCTCTGGCTGAAGTGCAACACAAACCTGATCATCTTCAATACCGTTACGATCCTGATCATTGCAAGTTCTTGGGAACATTGAAGAACCCTATATTACTAACAGTGGTTATGACGGATCCTGACGCTCCTGGAG
GCGAGTATTTGTACTGGTTATCTAGGCATTTCATTGCCGCCCAACAAGAACTAGTGGTGAAATGGCA

Intron 2 GTTATCAAGA ... TCTTATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.73	275.4	49	86
A2	16.89	199.6	51	30
A3	19.22	208	44	54.8
A4	22.46	195.4	35	78
A5	24.84	115.2	41	35
A6	17.7	1788.2	390	378.4
A7	18.3	1334.8	314	283.4
A8	19.16	1096.2	252	267.2
A9	17.5	1309.4	263	292
A10	18.71	1043.2	227	252.6

>TRINITY_DN48487_c1_g1_i10 from sample A10 / Example spliced isoform

CCCATACTATGGTACGATCTGCTTGACTACGAAGCTCCAAGGGAAAGACGCATGAGTACCCGCAAGATAGTTTTCACACTATTCATGACAGCGAATGTGCCGCACCGTATCATGCTTCCCCAGTCGCGGAAGAATTTCAGTACCAGACTTTTTGCAGACACTAATGCACTTGGACCTCCGCTGGCTGCTGCCTTTTTCAA
AATGATCGATCCGAATGAGAAGACAAAGGAGGAGGTGAAGCAGCTGCTTACAGAAGCTGGAGTTATCGGAGGGATTTTGGGCGATTTTGATCCTGATTTTCAATTGGATCTAATCTACAGCTCTACACACTTCGGCGATCAACGAATATGTCTAGGTGATGAAGTCTCGCGTTATCAACTGAATCACAGACCAAGTAGTT
TGGACTATATTCATGATCTTGATCATCCCGAGCTGCTGGACAACTTCATGGAGCCCGCATTACTCACAATTGTTATGACGGATCCTGACGCTCCTGG

>TRINITY_DN48487_c1_g1_i17 from sample A10 / Example unspliced isoform:

CTCTGTTTAAGTTGGATGTAATCTACCGCGATAAAAGAATAAGCCTAGGTGATTATGTCTCGCCTGATCAAGTTCAACACAGACCTCATCATTTTGAATACTTTTACGAATCTGCTAATACCCAGTTACCGGAATCCGTCATCCACGCCCCATCAATGGCAATGGTTATGACGGATCTTGACGCTGATGGCGGCGAGTAT
TTGCACTGGTTATGTGTGAATGTGAATCCCCGCCAAGAATTATTAGTATTACCGCCCACGATATTACCGCCCACGATATTACCGCCCACGATATTACCGCCCATACTATGGTACGATCTGCTTGACTACGAAGCTCCAAGGGAAAGACGCATGAGTACCCGCAAGATAGTTTTCACACTATTCATGACAGCGAATGTGCC
GCACCGTATCATGCTTCCCCAGTCGCGGAAGAATTTCAGTACCAGACTTTTTGCAGACACTAATGCACTTGGACCTCCGCTGGCTGCTGCCTTTTTCAAAATGATCGATCCGAATGAGAAGgttatcaagaattacccgcatgtaacggcggtgtacgttgagtcccgttgaatatctgtgaactggctacacgttttat
gacaaaaacccaaataaaattttaaccgccatgaaagtctaagccgtgcttattatcatttcaatagcttttaaacgttttaatgaggaggttgtgaaggttttctgagtttctcaacgtatttttatctgtaatttcttcatagaaatattccacagagatgtgttacatataacactgtgcttaacgttataatttta
tttaaaatatatctatttttttatttaagtttttaatggaattgatatttacttttactgatttaaataccgccgagcagttttcattgctttcacgagcgacctccatcatttaatgcttcttcatttgggatcttatgcagACAAAGGAGGAGGTGAAGCAGCTGCTTACAGAAGCTGGAGTTATCGGAGGGATTTTG
GGCGATTTTGATCCTGATTTTCAATTGGATCTAATCTACAGCTCTACACACTTCGGCGATCAACGAATATGTCTAGGTGATGAAGTCTCGCGTTATCAACTGAATCACAGACCAAGTAGTTTGGACTATATTCATGATCTTGATCATCCCGAGCTGCTGGACAACTTCATGGAGCCCGCATTACTCACAATTGTTATGAC
GGATCCTGACGCTCCTGGCGGCGAGTATTTGCACTGGCTATGTAGGCAATTCATCGTCGCCGAACAAGAAGAAGATAAGCCATCGGAGGAGCTGGTTTCCTATGAAGCTCCAAGGGAGGGAACCCGGAAGATAGTTTGCACAGTATTCAAACCGAATGAATCTCAGCCCAATACCAATATCGGTATGACATTCCCCAATA
TCGTTATACCCGAGTCGCGCAACAATTTCAGTACGAGGGATTTTGAATCCACAAATGCAGTTGCTCCGCTAGCTGCTGCGTTTTTTGAAATCAACTATCCGAATGAGAATGGTCATCAAGAATTGCCCATGTAACGGCCATGTAAGTTGATTGCCATTTGAATTTCTGTGAACTGGCTACACGTTTTATGACAAAAACCC
GAATAGTTTGAATTTCTGTGAACTGGCTACACGTTTTATGACAAAAACCAGAATAGTTTGAATTTCTGTGAACTGGCTACACGTTTTATGACACACTAATGCACTTGGACCTCCGCTGGCTGCTGCTTTTTTCAACATCAGCAACCAGAATAAGAATAATGATAAAGAATCACACACTTGTAACGGCCATGTACTTTGAG
TACCATTTGAATATCTGCGATAAAGAATCACACACTTGTAACAAGAATAGAATTTTGACAGCCGTGAGAATCTCAGCCGTGTTTATTATCGTGTCAGTAGATTGTTAACGTTTCAAACAAGTGATTGCGAGGTATGTGATGTGACAGTCGGTCCATCCGTCTTTTCTGTATAACTTTTAAATCTATAATGTGAAATTAGG
CACAAAGGTTCTATTGTTTATCTTTTTCATGCTTAAGTTTTAAATCAATTCGACTCAATTCGAC

Intron 3 GTATGCTATA ... CGTTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.64	263	12	86
A2	25.95	234	8	82
A3	24.57	261	4	85
A4	25.87	149	4	52
A5	22.58	144	8	42
A6	24.43	2506	12	810
A7	24.79	1754.3	54	578
A8	24.11	2018	35	641
A9	25.95	1758	37	616
A10	25.43	1158.5	57	395

>TRINITY_DN48487_c1_g1_i23 from sample A10 / Example spliced isoform

CTCTGTTTAAGTTGGATGTAATCTACCGCGATAAAAGAATAAGCCTAGGTGATTATGTCTCGCCTGATCAAGTTCAACACAGACCTCATCATTTTGAATACTTTTACGAATCTGCTAATACCCAGTTACCGGAATCCGTCATCCACGCCCCATCAATGGCAATGGTTATGACGGATCTTGACGCTGATGGCGGCGAGTAT
TTGCACTGGTTATGTGTGAATGTGAATCCCCGCCAAGAATTATTAGTATTACCGCCCACGATATTACCGCCCACGATATTACCGCCCACGATATTACCGCCCATACTATGGTACGATCTGCTTGACTACGAAGCTCCAAGGGAAAGACGCATGAGTACCCGCAAGATAGTTTTCACACTATTCATGACAGCGAATGTGCC
GCACCGTATCATGCTTCCCCAGTCGCGGAAGAATTTCAGTACCAGACTTTTTGCAGACACTAATGCACTTGGACCTCCGCTGGCTGCTGCCTTTTTCAAAATCATCGATCCGAATGAGAAGACAAAGGAGGAGGTGAAGCAGCTGCTTACAGAAGCGGGAGTTATCGGAGGGATTTTGGGCGATTTTGATCCTGATTTTC
AATTGGATCTAATCTACAGCTCTACACACTTCGGCGATCAACGAATATGTCTAGGTGATGAAGTCTCGCGTTATCAACTGAATCACAGACCTAGTAGTTTGGACTACATTCATGATCTTGATCATCCCGAGCTGCTGGACAACTTCATGGAGCCCGCATTACTCACAATTGTTATGACGGATCCTGACGCTCCTGGCGGC
GAGTATTTGCACTGGCTATGTAGGCAATTCATCGTCGCCGAACAAGAAGAAGATAAGCCATCGGAGGAGCTGGTTTCCTATGAAGCTCCAAGGGAGGGAACCCGGAAGATAGTTTGCACAGTATTCAAACCGAATGAATCTCAGCCCAATACCAATATCGGTATGACATTCCCCAATATCGTTATACCCGAGTCGCGCAA
CAATTTCAGCACCAGAGATTTTGCATCCACTCATGCACTTGGACCTCCGCTGGCTAGTACTTTTGTCAAAATCAACAATCGGCATCTAGCTAATTGTCTACTGCAGTTTCTGCCAACTGGCTAGAATGCAAGTCTCAACCGCGTCTATTATCATTTCAATAGATTTTGAATGTTTCAGTCAGGAGTTTTTGAAGGTTTCC
AGAGTTTCTTAACGTAATTTTATCTATCATTTCTTAATAGAATCATTTGACCAGAGATGTGTTACATGTTATCTGTCATTTCTTAATAGAATTATTCACTAATGCACTTGGACCTCCGCTGGCTGCTGCTTTTTTCAACATCAGCAACCAGAATAAGAATAATGATAAAGAATCACACACTTGTAACGGCCATGTACTTT
GAGTACCATTTGAATATCTGCGATAAAGAATCACACACTTGTAACAAGAATAGAATTTTGACAGCCGTGAGAATCTCAGCCGTGTTTATTATCGTGTCAGTAGATTGTTAACGTTTCAAACAAGTGATTGCGAGGTATGTGATGTGACAGTCGGTCCATCCGTCTTTTCTGTATAACTTTTAAATCTATAATGTGAAATT
AGGCACAAAGGTTCTATTGTTTATCTTTTTCATGCTTAAGTTTTAAATCAATTCGACTCAATTCGAC

>TRINITY_DN48487_c1_g1_i18 from sample A10 / Example unspliced isoform:

ATTTGGTCAAATTGAAATTCATATTCTCAAGCATTTCTTGAACCCGTAACAAGGTTATGACGGATCCTGACGCTCCTGGCGGCGAGTATTTGCACTGgtatgctataaacctcgtgaagttacatcaaattaatgattgtagagcaccggaatccattaaataactattcatcgcaacgttgtgcagGCTATGTAGGCAA
TTCATCGTCGCCGAACAAGAAGAAGATAAGCCATCGGAGGAGCTGGTTTCCTATGAAGCTCCAAGGGAGGGAACCCGGAAGATAGTTTGCACAGTATTCAAACCGAATGAATCTCAGCCCAATACCAATATCGGTATGACATTCCCCAATATCGTTATACCCGAGTCGCGCAACAATTTCAG

Intron 4 GTACGTTAGT ... TTTTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.65	112	8	24
A2	24.14	44	3	14
A3	14.29	42	7	7
A4	27.54	50	7	19
A5	46.15	7	2	6
A6	21.38	250	17	68
A7	17.58	286	17	61
A8	21.75	367	13	102
A9	20.39	289	39	74
A10	17	249	11	51

>TRINITY_DN48487_c1_g1_i9 from sample A10 / Example spliced isoform

TTTCAGTACCAGAGATTTTGCATATTCTAATGCACTCGGAGCTCCGCTGGCTGCTGCTTTTTTCAAGATCAACAATCAGAATCAGAATGAGGATAAAGAATCATCCACATGTAAAGGCTATAAAGTGGAGGATATAAAACAGAAACTTGTGGAAGCGGGAGTTATCGGACGTGTTGTCGACGATTTCAATCCTCATATAA
AATTAAGTGTATTCTACGGCGATAAACATATAACCCTAGGTACTGAAGTCACGCCTGCTGAAGTGCAACATAAACCTGATCATTTGCAATACCGTTACTATCCCAATCATTCCAAGTTCTTGGCATCATTGAGGAACCCCATATTACTAACAGTGGTTATGACGGATCCTGACGCTCCTGGAGGCGAGTATTTATACTGG
TTAAATCGGCATTTCGTTTCCGCCCAACCAGAAGGTATCATGGTTGTGTTTCTTTTCTTGAG

>TRINITY_DN48487_c1_g1_i19 from sample A10 / Example unspliced isoform:

TTTCAGTACCAGAGATTTTGCATATTCTAATGCACTCGGAGCTCCGCTGGCTGCTGCTTTTTTCAAGATCAACAATCAGAATCAGAATGAGGATAAAGAATCATCCACATGTAAAGGCTATgtacgttagtgccgtttgattatcagtgaattggctataccttttatgatcaaaattttcgcagccatgaaagtctcag
ccatcttggtgcattaattttgtgcagAAAGTGGAGGATATAAAACAGAAACTTGTGGAAGCGGGAGTTATCGGACGTGTTGTCGACGATTTCAATCCTCATATAAAATTAAGTGTATTCTACGGCGATAAACATATAACCCTAGGTACTGAAGTCACGCCTGCTGAAGTGCAACATAAACCTGATCATTTGCAATACCG
TTACTATCCCAATCATTCCAAGTTCTTGGCATCATTGAGGAACCCCATATTACTAACAGTGGTTATGACGGATCCTGACGCTCCTGGAGGCGAGTATTTATACTGGTTAAATCGGCATTTCGTTTCCGCCCAACCAGAAGGTATCATGGTTGTGTTTCTTTTCTTGAG

Intron 5 GTATCATGGT ... GCAATATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.64	10.5	7.5	5
A2	31.7	15	13.5	4
A3	61.98	7	15	4
A4	36.66	10.5	9	4
A5	94.12	0.5	7	3
A6	56.66	63	95	55
A7	41.98	213	140.5	161
A8	38.05	238	140	148
A9	37.05	365	179.5	235
A10	47.75	223	190	201.5

>TRINITY_DN46360_c3_g3_i4 from sample A8 / Example spliced isoform

CCTGGCGGCGAGTATTTGCACTGGCTATGTAGGCATTTCACGGCCGTCCCACAAGAAAGAGATATGACATGGCAGGAGCTGCTTACCTATGAACCTACAACGGCAAGACACATGGGAACACGCAAGATAGTTTTCACAGCATTCAAACCGAATGAACCTCTGCCCAATGCCAATATCGGTATGATTATGCCCAATATCAT
TATGCCCGAGTCGCGGAACAATTTCAGTACCAGAGATTTTGCATCAACTAATTCACTTGGAGCTCCGGTGGCTGCTGCTTTTTTCAAAATCATCGATCCGG

>TRINITY_DN46360_c3_g3_i1 from sample A8 / Example unspliced isoform:

CCTGGCGGCGAGTATTTGCACTGGCTATGTAGGCATTTCACGGCCGTCCCACAAGAAAGAGgtatcatggttgtgtttattattttgttgacctagaaatcgatcgatcgaatgcgctccgtaatgttaaccgtcgtggatgttgcgctgcaatattagATATGACATGGCAGGAGCTGCTTACCTATGAACCTACAACG
GCAAGACACATGGGAACACGCAAGATAGTTTTCACAGCATTCAAACCGAATGAACCTCTGCCCAATGCCAATATCGGTATGATTATGCCCAATATCATTATGCCCGAGTCGCGGAACAATTTCAGTACCAGAGATTTTGCATCAACTAATTCACTTGGAGCTCCGGTGGCTGCTGCTTTTTTCAAAATCATCGATCCGG