NA ----> NA

Intron 1 GTAGGTGGAG ... TAAGAAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.52	73	54	27
A2	48.91	47	45	20
A3	45.65	75	63	11
A4	54.72	24	29	12
A5	47.62	11	10	5
A6	47.75	58	53	19
A7	43.57	307	237	50
A8	40.83	326	225	54
A9	47.33	128	115	32
A10	44.81	271	220	67

>TRINITY_DN130677_c2_g1_i8 from sample A1 / Example spliced isoform

TTCAGGGAGCATATTTTACTGTCTGTTTGATTATTATTTTGTATTTGTGTTTTTTGTTTCGAGCATGGCCATGCTTATTTGATTTCCGTTTTGATTTCCTGTGTGGATTCTGTTTTCAATTCTTTCTATTCTCTTCTTTTTTAATTTTTATTTTCTATAATTCTTTCGTTTTCGTTTTGGGTTTGCTTTTTTTTGTCTCT
GTTTTGAGTCTGGTTTGTTTGAGATTAAGAGGGAGGATGGAGGACTTAAAATATCCAGAAGTGAGCATCACTGGATGGTCAATTGCGAAGGCGGAGCAGATTCAGAGCTACACGAACAATAATTAGGTGCAGAAATCGCTGGTCCCGCGGAATTTGAGGATTACAGGTGAGGTGGAGAAGAGTTCTGGCCATATTTTGAA
GTGCCTTGAGAAATATAAGAAAAAGGTCTAATAGGTGGGTGGGTGGATGAAGACTGCAAGTTATTTTTGCATAGACAGAATAATTGAAGAAGAGATGGAAAGATAGAAAGC

>TRINITY_DN130677_c2_g1_i11 from sample A1 / Example unspliced isoform:

TTCAGGGAGCATATTTTACTGTCTGTTTGATTATTATTTTGTATTTGTGTTTTTTGTTTCGAGCATGGCCATGCTTATTTGATTTCCGTTTTGATTTCCTGTGTGGATTCTGTTTTCAATTCTTTCTATTCTCTTCTTTTTTAATTTTTATTTTCTATAATTCTTTCGTTTTCGTTTTGGGTTTGCTTTTTTTTGTCTCT
GTTTTGAGTCTGGTTTGTTTGAGATTAAGAGGGAGGATGGAGGACTTAAAATATCCAGAAGTGAGCATCACTGGATGGTCAATTGCGAAGGCGGAGCAGATTCAGAGCTACACGAACAATAATTAGGTGCAGAAATCGCTGGTCCCGCGGAATTTGAGGATTACAGGTGAGGTGGAGAAGAGTTCTGGCCATATTTTGAA
GTGCCTTGAGAAATATAAGAAAAAGGTCTAATAGgtaggtggagaagactgcaagctattttgaagtgcctttaaataaattaagaaaaagGTGGGTGGGTGGATGAAGACTGCAAGTTATTTTTGCATAGACAGAATAATTGAAGAAGAGATGGAAAGATAGAAAGC

Intron 2 GTTTGTGCTC ... CGGAGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.29	289	114	18
A2	30.82	229	102	11.5
A3	27.25	323	121	16.5
A4	27.66	221	84.5	17.5
A5	45.1	84	69	10
A6	25.43	302	103	15.5
A7	22.06	1434.5	406	71.5
A8	23.44	1388	425	64
A9	21.59	552	152	26
A10	22.58	1368	399	56

>TRINITY_DN130677_c2_g1_i12 from sample A1 / Example spliced isoform

TTCAGGGAGCATATTTTACTGTCTGTTTGATTATTATTTTGTATTTGTGTTTTTTGTTTCGAGCATGGCCATGCTTATTTGATTTCCGTTTTGATTTCCTGTGTGGATTCTGTTTTCAATTCTTTCTATTCTCTTCTTTTTTAATTTTTATTTTCTATAATTCTTTCGTTTTCGCTTTGGGTTTGCTATTTTTTGTCTCT
GTTTTGAGTCTGGTTTGTTTGAGATTAAGAGGGAGGATGGAGGACTACAAATATCACTTAGTCTGGTTTGTTTGGGATTAAGAGGGAGTATGGAGGACTAAAAATATCCAGAAGTGAGCATCACTGGATGGTCAATTGCGAAGGCGGAGAAGATTGAGAGCTACAAGAGCAATAATTAGGTGCAGAAATCGCTGATCCCG
CGGAATTTGAGGATTACAGGTGAGGTGGAGAAGAGTTCTGGCCATATTTTGAAGTGCCTTGAGAAATATCAGAAAAGGTCTAACAGGTGGGTGGGTGGATGAAGACTGCAAGTTATTTTTGCATAGACAGAATAATTGAAGAAGAGATGGAAAGATAGAAAGC

>TRINITY_DN130677_c2_g1_i7 from sample A1 / Example unspliced isoform:

TTCAGGGAGCATATTTTACTGTCTGTTTGATTATTATTTTGTATTTGTGTTTTTTGTTTCGAGCATGGCCATGCTTATTTGATTTCCGTTTTGATTTCCTGTGTGGATTCTGTTTTCAATTCTTTCTATTCTCTTCTTTTTTAATTTTTATTTTCTATAATTCTTTCGTTTTCGCTTTGGGTTTGCTATTTTTTGTCTCT
GTTTTGAGTCTGGTTTGTTTGAGATTAAGAGGGAGGATGGAGGACTACAAATATCACTTAGTCTGGTTTGTTTGGGATTAAGAGGGAGTATGGAGGACTAAAAATATCCAGAAGTGAGCATCACTGGATGGTCAATTGCGAAGGCGGAGAAGgtttgtgctcatttaattaatgtgtaaatatctggattgaaatttaat
ccctaatgcattctttcatcatgatctcttttgtccatattttaatcatgatctgttttgtcccggagtgcagATTGAGAGCTACAAGAGCAATAATTAGGTGCAGAAATCGCTGATCCCGCGGAATTTGAGGATTACAGGTGAGGTGGAGAAGAGTTCTGGCCATATTTTGAAGTGCCTTGAGAAATAT

Intron 3 GTTTGTGCTC ... CGGAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.22	193	34	32
A2	14.63	140	26	24
A3	13.52	211	35	33
A4	15.24	139	15	25
A5	28.33	43	34	17
A6	11.42	194	5	25
A7	11	979	15	121
A8	11.96	883	18	120
A9	11.33	368	3	47
A10	12.61	901	4	130

>TRINITY_DN119047_c2_g1_i7 from sample A3 / Example spliced isoform

AGAAAAGGTTGACAGATAGAGAGGAGCCGAGAAGATTTCAGGGAGCATATTTTACTGTCTGTTTGATTATTATTTTGTATTTGTGTTTTTTGTTTCGAGCATGGCCATGCTTATTTGATTTCCGTTTTGATTTCCTGTGTGGATTCTGTTTTCAATTCTTTCTATTCTCTTCTTTTTTAATTTTTATTTTCTATAATTCT
TTCGTTTTCGCTTTGGGTTTGCTATTTTTTGTCTCTGTTTTGAGTCTGGTTTGTTTGAGATTAAGAGGGAGGATGGAGGACTTAAAATATCCAGAAGTGAGCATCACTGGATGGTCAATTGCGAAGGCGGAGCAGATTCAGAGCTACACGAACAATAATTAGGTGCAGAAATCGCTGGTCCCGCGGAATTTGAGGATTAC
AGGTGAGGTGGAGAAGAGTTCTGGCCATATTTTGAAGTGCCTTGAGAAATATAAGAAAAAGGTCTAATAGGTAGGTGGAGAAGACTGCAAGCTATTTTGAAGTGCCTTTAAATAAATTAAGAAAAAGGTAAACAATTACATTTTTTTTTTGCGTACAGGTGGGTGGGTGGATGAAGACT

>TRINITY_DN119047_c2_g1_i5 from sample A3 / Example unspliced isoform:

AGAAAAGGTTGACAGATAGAGAGGAGCCGAGAAGATTTCAGGGAGCATATTTTACTGTCTGTTTGATTATTATTTTGTATTTGTGTTTTTTGTTTCGAGCATGGCCATGCTTATTTGATTTCCGTTTTGATTTCCTGTGTGGATTCTGTTTTCAATTCTTTCTATTCTCTTCTTTTTTAATTTTTATTTTCTATAATTCT
TTCGTTTTCGCTTTGGGTTTGCTATTTTTTGTCTCTGTTTTGAGTCTGGTTTGTTTGAGATTAAGAGGGAGGATGGAGGACTTAAAATATCCAGAAGTGAGCATCACTGGATGGTCAATTGCGAAGGCGGAGCAGgtttgtgctcatttaattaatgtgtaaatatctggattgaaatttaatccctaatgcattttttc
atcatgatctgttttgtcccggaatgcagATTCAGAGCTACACGAACAATAATTAGGTGCAGAAATCGCTGGTCCCGCGGAATTTGAGGATTACAGGTGAGGTGGAGAAGAGTTCTGGCCATATTTTGAAGTGCCTTGAGAAATATAAGAAAAAGGTCTAATAGGTAGGTGGAGAAGACTGCAAGCTATTTTGAAGTGCC
TTTAAATAAATTAAGAAAAAGGTAAACAATTACATTTTTTTTTTGCGTACAGGTGGGTGGGTGGATGAAGACT