NA ----> NA

Intron 1 GTTTAAGTTC ... GGTTGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.81	35	9	6
A2	11.54	32	4	0
A3	15.76	27	5	0
A4	19.23	26.5	6	5
A5	16.23	15.5	3	0
A6	17.34	108	22	5
A7	14.54	127.5	21	0
A8	11.72	179	23	3
A9	11.82	138.5	18	1
A10	8.11	140.5	12	4

>TRINITY_DN122353_c0_g1_i5 from sample A1 / Example spliced isoform

GTTTCATTTCCCCGCTTCGGGGTATGGTAGTTCATAAATCATTCAATTTGTTCCAACGGGTTCTCGCTCGAACAAATCTTGTTTCCTGGGTCCTAAAAACCAAGGTATCTTACATACTATGCCTTAAAGCCCTAGGCGAACAACCATGCCGCCGAGAAGGAGGTAGAAGAGAAGGATAGTGATGCCGGTCCATCCTACGT
CCCACCCGCTACGCCCATTGTTCTTTGTCAGAATCCAGTGATCACTGTTGCTTATAATGCTAGTGTGTGTTTGATTAGCAGAACTTTTTGCCCCAACCTGCTAGGTGCCGGAGCCCAGAATCAATAATGCCATGAACAGGCTGATCGGGGAAGGCAAAGCTAGCAGTTTTCCAGCCAGAAGATATCAAGCAGAGCAAAGA
TGATCAAGAGACAGTTTTACAGCATAGACCATCCCAATGACAACTGCGGTGCCTCTGATAATTCTTCATCATCTTCAGAGGATGATCCTGAACCTACTCATGAACTTTCCACCGATGGCAAATCAGAAGAAGAAGAAACAGCAGCAGCAGCAGCGGTGGAATCCCCAGAGTCCGGATACCAGAGTGAGAATAGCTCAGAG
AGTGATGAAGAGTTCGATGCCACTGAAGCTTGGCCAGGTCAGTTAACCAGTGATGACAATGGCAAGTCTGAATTGCTTCCAGACAGTTTTAAACAAAAGAGGAGCTGTGATTCAATAGATAGAGAAAACATAACTATTGCAATAGCCCAGGAAAATCATTCTAATCAGTGGGCAGAAGCTGTAGAAGAGACAGAGAAGGA
CCGGGCTTTGCATAACTTAGATGATTGTGTTATAAAGTGCAAATCAGTCTTTAAGTGCAAGATCTGTCCAAGAATTGTTTGTCTATCTGTGGACACAATGGAAGCACACCTTGTTTCAAAGAGGCATGCACGGTCCCAAAAGCTATTTGCTGAAGGAAGGTTGAAGTTGATGCTTAACAGTGATGGAGAAGTGGAGGAAG
ATGCTGAAACTCATGCAGAAAGGCATGCCCGGATAGTTGCTGCTGTGGAGCCAACTAAAAACTTGAAGAAAAGAAACAGAGGCCGGCAACGCCAGAAAAAACGAAAGAAGAAAACTTCCCAGACTGGAAGCAAAAATGCTTAAGGGAAGTTCTCATCAGAGGTTTCTACAAAGGCTGGGAAAATATGAGCATTGCAAAAG
CATCTATATTGGTTTTGGCAATGTTTGGCCGGAGATGGATGTTTTACATGTTTTTCAGCCAGTGAAAAAGGTTTTTATTTTGAGATGACTACCACATTTGAAAACAGAGTGAAATTGCAAACAAGCTTAAAAAGCTTTCCTTCTGAGCATGTTTATCAGTTTTTCTCAGATGAGAAGCCATAAAAATAAAACTTTTATTT
GAAGGATTTTTTAGTGACTGGAAAAGAAAAGACTGAAATGTAACTTACTTATCAAGCTTTTTTCTGAATATGCATTCATGCTACTTGAACCTAATGCTTTATAGGAGCAATGGATACGCAATCGAGTGTATAAAGAATTTGTAACTGTAATCTAGAATTCATGGACGACGGCTTATATTGCCTTATCATTGATTTTTATG
GAAATGTTTTGGTTTAGTATTTAATTATAAGAGAGCAGTTGATTGCCTCCTGCCTCATAGACTTTTATTTGGGTGTAGACGGTCACCCCTACTTGGC

>TRINITY_DN122353_c0_g1_i4 from sample A1 / Example unspliced isoform:

GTTTCATTTCCCCGCTTCGGGGTATGGTAGTTCATAAATCATTCAATTTGTTCCAACGGGTTCTCGCTCGAACAAATCTTGTTTCCTGGGTCCTAAAAACCAAGGTATCTTACATACTATGCCTTAAAGCCCTAGGCGAACAACCATGCCGCCGAGAAGGAGGTAGAAGAGAAGGATAGTGATGCCGGTCCATCCTACGT
CCCACCCGCTACGCCCATTGTTCTTTGTCAGAATCCAGTGATCACTGTTGCTTATAATGCTAGTGTGTGTTTGATTAGCAGAACTTTTTGCCCCAACCTGCTAGGTGCCGGAGCCCAGAATCAATAATGCCATGAACAGGCTGATCGGGGAAGGCAAAGCTAGCAGTTTTCCAGCCAGAAGATATCAAGCAGAGCAAAGA
TGATCAAGAGACAGTTTTACAGCATAGACCATCCCAATGACAACTGCGGTGCCTCTGATAATTCTTCATCATCTTCAGAGGATGATCCTGAACCTACTCATGAACTTTCCACCGATGGCAAATCAGAAGAAGAAGAAACAGCAGCAGCAGCAGCGGTGGAATCCCCAGAGTCCGGATACCAGAGTGAGAATAGCTCAGAG
AGTGATGAAGAGTTCGATGCCACTGAAGCTTGGCCAGGTCAGTTAACCAGTGATGACAATGGCAAGTCTGAATTGCTTCCAGACAGTTTTAAACAAAAGAGGAGCTGTGATTCAATAGATAGAGAAAACATAACTATTGCAATAGCCCAGGAAAATCATTCTAATCAGTGGGCAGAAGCTGTAGAAGAGACAGAGAAGGA
CCGGGCTTTGCATAACTTAGATGATTGTGTTATAAAGTGCAAATCAGTCTTTAAGTGCAAGATCTGTCCAAGAATTGTTTGTCTATCTGTGGACACAATGGAAGCACACCTTGTTTCAAAGAGGCATGCACGGTCCCAAAAGCTATTTGCTGAAGGAAGGTTGAAGTTGATGCTTAACAGTGATGGAGAAGTGGAGGAAG
ATGCTGAAACTCATGCAGAAAGGCATGCCCGGATAGTTGCTGCTGTGGAGgtttaagttcttcactttgacaatcatattcttccgattttttcctgttatatacatttatttggagatgtgtaacatgtgttgatcttttgggttgcacagCCAACTAAAAACTTGAAGAAAAGAAACAGAGGCCGGCAACGCCAGAAA
AAACGAAAGAAGGTGGATGATTTCAATTCTGTACATGGTAATTTTTCTCTTCTCTCTAGTCTTTATATGTTTGTTTGACATGATATTATTGTTCTTTCCAGAAAACTTCCCAGACTGGAAGCAAAAATGCTTAAGGGAAGTTCTCATCAGAGGTTTCTACAAAGGCTGGGAAAATATGAGCATTGCAAAAGCATCTATAT
TGGTTTTGGCAATGTTTGGCCGGAGATGGATGTTTTACATGTTTTTCAGCCAGTGAAAAAGGTTTTTATTTTGAGATGACTACCACATTTGAAAACAGAGTGAAATTGCAAACAAGCTTAAAAAGCTTTCCTTCTGAGCATGTTTATCAGTTTTTCTCAGATGAGAAGCCATAAAAATAAAACTTTTATTTGAAGGATTT
TTTAGTGACTGGAAAAGAAAAGACTGAAATGTAACTTACTTATCAAGCTTTTTTCTGAATATGCATTCATGCTACTTGAACCTAATGCTTTATAGGAGCAATGGATACGCAATCGAGTGTATAAAGAATTTGTAACTGTAATCTAGAATTCATGGACGACGGCTTATATTGCCTTATCATTGATTTTTATGGAAATGTTT
TGGTTTAGTATTTAATTATAAGAGAGCAGTTGATTGCCTCCTGCCTCATAGACTTTTATTTGGGTGTAGACGGTCACCCCTACTTGGC

Intron 2 GTGGATGATT ... TTCTTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.83	57	6.9	15
A2	19.51	33	7.3	8
A3	30.56	25	7.5	11
A4	15.63	27	7.5	5
A5	11.11	8	4.4	1
A6	31.87	171	25.2	80
A7	20.5	151.3	31.6	39
A8	22.01	202	44.8	57
A9	27.85	189.1	39.3	73
A10	20.78	183	39.2	48

>TRINITY_DN122353_c0_g1_i5 from sample A1 / Example spliced isoform

GTTTCATTTCCCCGCTTCGGGGTATGGTAGTTCATAAATCATTCAATTTGTTCCAACGGGTTCTCGCTCGAACAAATCTTGTTTCCTGGGTCCTAAAAACCAAGGTATCTTACATACTATGCCTTAAAGCCCTAGGCGAACAACCATGCCGCCGAGAAGGAGGTAGAAGAGAAGGATAGTGATGCCGGTCCATCCTACGT
CCCACCCGCTACGCCCATTGTTCTTTGTCAGAATCCAGTGATCACTGTTGCTTATAATGCTAGTGTGTGTTTGATTAGCAGAACTTTTTGCCCCAACCTGCTAGGTGCCGGAGCCCAGAATCAATAATGCCATGAACAGGCTGATCGGGGAAGGCAAAGCTAGCAGTTTTCCAGCCAGAAGATATCAAGCAGAGCAAAGA
TGATCAAGAGACAGTTTTACAGCATAGACCATCCCAATGACAACTGCGGTGCCTCTGATAATTCTTCATCATCTTCAGAGGATGATCCTGAACCTACTCATGAACTTTCCACCGATGGCAAATCAGAAGAAGAAGAAACAGCAGCAGCAGCAGCGGTGGAATCCCCAGAGTCCGGATACCAGAGTGAGAATAGCTCAGAG
AGTGATGAAGAGTTCGATGCCACTGAAGCTTGGCCAGGTCAGTTAACCAGTGATGACAATGGCAAGTCTGAATTGCTTCCAGACAGTTTTAAACAAAAGAGGAGCTGTGATTCAATAGATAGAGAAAACATAACTATTGCAATAGCCCAGGAAAATCATTCTAATCAGTGGGCAGAAGCTGTAGAAGAGACAGAGAAGGA
CCGGGCTTTGCATAACTTAGATGATTGTGTTATAAAGTGCAAATCAGTCTTTAAGTGCAAGATCTGTCCAAGAATTGTTTGTCTATCTGTGGACACAATGGAAGCACACCTTGTTTCAAAGAGGCATGCACGGTCCCAAAAGCTATTTGCTGAAGGAAGGTTGAAGTTGATGCTTAACAGTGATGGAGAAGTGGAGGAAG
ATGCTGAAACTCATGCAGAAAGGCATGCCCGGATAGTTGCTGCTGTGGAGCCAACTAAAAACTTGAAGAAAAGAAACAGAGGCCGGCAACGCCAGAAAAAACGAAAGAAGAAAACTTCCCAGACTGGAAGCAAAAATGCTTAAGGGAAGTTCTCATCAGAGGTTTCTACAAAGGCTGGGAAAATATGAGCATTGCAAAAG
CATCTATATTGGTTTTGGCAATGTTTGGCCGGAGATGGATGTTTTACATGTTTTTCAGCCAGTGAAAAAGGTTTTTATTTTGAGATGACTACCACATTTGAAAACAGAGTGAAATTGCAAACAAGCTTAAAAAGCTTTCCTTCTGAGCATGTTTATCAGTTTTTCTCAGATGAGAAGCCATAAAAATAAAACTTTTATTT
GAAGGATTTTTTAGTGACTGGAAAAGAAAAGACTGAAATGTAACTTACTTATCAAGCTTTTTTCTGAATATGCATTCATGCTACTTGAACCTAATGCTTTATAGGAGCAATGGATACGCAATCGAGTGTATAAAGAATTTGTAACTGTAATCTAGAATTCATGGACGACGGCTTATATTGCCTTATCATTGATTTTTATG
GAAATGTTTTGGTTTAGTATTTAATTATAAGAGAGCAGTTGATTGCCTCCTGCCTCATAGACTTTTATTTGGGTGTAGACGGTCACCCCTACTTGGC

>TRINITY_DN122353_c0_g1_i4 from sample A1 / Example unspliced isoform:

GTTTCATTTCCCCGCTTCGGGGTATGGTAGTTCATAAATCATTCAATTTGTTCCAACGGGTTCTCGCTCGAACAAATCTTGTTTCCTGGGTCCTAAAAACCAAGGTATCTTACATACTATGCCTTAAAGCCCTAGGCGAACAACCATGCCGCCGAGAAGGAGGTAGAAGAGAAGGATAGTGATGCCGGTCCATCCTACGT
CCCACCCGCTACGCCCATTGTTCTTTGTCAGAATCCAGTGATCACTGTTGCTTATAATGCTAGTGTGTGTTTGATTAGCAGAACTTTTTGCCCCAACCTGCTAGGTGCCGGAGCCCAGAATCAATAATGCCATGAACAGGCTGATCGGGGAAGGCAAAGCTAGCAGTTTTCCAGCCAGAAGATATCAAGCAGAGCAAAGA
TGATCAAGAGACAGTTTTACAGCATAGACCATCCCAATGACAACTGCGGTGCCTCTGATAATTCTTCATCATCTTCAGAGGATGATCCTGAACCTACTCATGAACTTTCCACCGATGGCAAATCAGAAGAAGAAGAAACAGCAGCAGCAGCAGCGGTGGAATCCCCAGAGTCCGGATACCAGAGTGAGAATAGCTCAGAG
AGTGATGAAGAGTTCGATGCCACTGAAGCTTGGCCAGGTCAGTTAACCAGTGATGACAATGGCAAGTCTGAATTGCTTCCAGACAGTTTTAAACAAAAGAGGAGCTGTGATTCAATAGATAGAGAAAACATAACTATTGCAATAGCCCAGGAAAATCATTCTAATCAGTGGGCAGAAGCTGTAGAAGAGACAGAGAAGGA
CCGGGCTTTGCATAACTTAGATGATTGTGTTATAAAGTGCAAATCAGTCTTTAAGTGCAAGATCTGTCCAAGAATTGTTTGTCTATCTGTGGACACAATGGAAGCACACCTTGTTTCAAAGAGGCATGCACGGTCCCAAAAGCTATTTGCTGAAGGAAGGTTGAAGTTGATGCTTAACAGTGATGGAGAAGTGGAGGAAG
ATGCTGAAACTCATGCAGAAAGGCATGCCCGGATAGTTGCTGCTGTGGAGGTTTAAGTTCTTCACTTTGACAATCATATTCTTCCGATTTTTTCCTGTTATATACATTTATTTGGAGATGTGTAACATGTGTTGATCTTTTGGGTTGCACAGCCAACTAAAAACTTGAAGAAAAGAAACAGAGGCCGGCAACGCCAGAAA
AAACGAAAGAAGgtggatgatttcaattctgtacatggtaatttttctcttctctctagtctttatatgtttgtttgacatgatattattgttctttccagAAAACTTCCCAGACTGGAAGCAAAAATGCTTAAGGGAAGTTCTCATCAGAGGTTTCTACAAAGGCTGGGAAAATATGAGCATTGCAAAAGCATCTATAT
TGGTTTTGGCAATGTTTGGCCGGAGATGGATGTTTTACATGTTTTTCAGCCAGTGAAAAAGGTTTTTATTTTGAGATGACTACCACATTTGAAAACAGAGTGAAATTGCAAACAAGCTTAAAAAGCTTTCCTTCTGAGCATGTTTATCAGTTTTTCTCAGATGAGAAGCCATAAAAATAAAACTTTTATTTGAAGGATTT
TTTAGTGACTGGAAAAGAAAAGACTGAAATGTAACTTACTTATCAAGCTTTTTTCTGAATATGCATTCATGCTACTTGAACCTAATGCTTTATAGGAGCAATGGATACGCAATCGAGTGTATAAAGAATTTGTAACTGTAATCTAGAATTCATGGACGACGGCTTATATTGCCTTATCATTGATTTTTATGGAAATGTTT
TGGTTTAGTATTTAATTATAAGAGAGCAGTTGATTGCCTCCTGCCTCATAGACTTTTATTTGGGTGTAGACGGTCACCCCTACTTGGC