sp|P27323|HS901_ARATH ----> Heat shock protein 90-1

Intron 1 GTGAGTTTTT ... CTCAATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	24.44	1.7	1	0
A3	33.33	1	0	1
A4	77.78	0.7	3	1
A5	25	3	0	2
A6	32.56	9.3	1	8
A7	28.5	21.3	8	9
A8	27.01	19	6	8
A9	31.11	15.7	4	10
A10	33.33	2	1	1

>TRINITY_DN43478_c4_g1_i8 from sample A7 / Example spliced isoform

GTTTACAGTTACCAGAGATGTCGACGGAGAGCCCCTCGGAAGGGGAACTAAAATGACTCTGTTTCTAAAGGAGGACCAGTGTGCTATTGGATAGACTTTGACCATCCACAACGATTGTCTGGTGTTGATGCATCGACTATCAATTGATCAGTTGTTGTGGCCTCGTAGAAACTTGTGAATGGAGGGATCAACATGCCAAC
ACCTTTGATCTTTTGAGTACCAGTGTATCTCTGATTCTCAAAGAAAATGTAATTGCAGATCATTACTCCCATTGCATCATTTTCAAAGACTAATTGAAGTATCTTTAACTTGTTTGATTAGTGTGTGTTTGCAATTTGTTATTTAACAAACTCTTGCAAAAATGGTCAAGGCATCCATTATTATGGCCTTGAACTAGGAT
GCTCAATTGATGGATCCACCTTGGAACACATTGCCTATAAGATTGACTTACTCATGCATGGCAGCCTCAAACTTCCTCGAGCATAGGGAAGAGGAAGATCAAAATTTTGGAGGCATATGGTAGTGGCAAGGGAAGTAATGGTCATTGATTTTTCATATTGTACTTTGGTACTTTTGGCATGAGATGCCCTTTATGTAATA
TGGGAACTAGTTGGCCATTTTGTACATGAGTTTTCATTATGTATTGGAGATATGATGTTGCATTTTTTAATACATGGATGAAATCAATATTTTGGAATACATGTGCATACTTTGGACATATTGTTGAG

>TRINITY_DN43478_c4_g1_i9 from sample A7 / Example unspliced isoform:

GCCGACATGCCGCCACTGGAGGAGGACGCCTCCGTCCAGGAGGAGAGCAAGATGGAGGAGTGTGCTATTGGATAGACTTTGACCATCCACAACGATTGTCTGGTGTTGATGCATCGACTATCAATTGATCAGTTGTTGTGGCCTCGTAGAAACTTGTGAATGGAGGGATCAACATGCCAACACCTTTGATCTTTTGAGTA
CCAGTGTATCTCTGATTCTCAAAGAAAATGTAATTGCAGATCATTACTCCCATTGCATCATTTTCAAAGACTAATTGAAGTATCTTTAACTTGTTTGATTAGTGTGTGTTTGCAATTTGTTATTTAACAAACTCTTGCAAAAATGGTCAAGGCATCCATTATTATGGCCTTGAACTAGGATGCTCAATTGATGGATCCAC
CTTGGAACACATTGCCTATAAGATTGACTTACTCATGCATGgtgagtttttgttgtattgaattgtagtttaataattatatatttgttcattttttaaatggtgtttcatatctaaactctaataatgaacatcaatcctcaatgtagGCAGCCTCAAACTTCCTCGAGCATAGGGAAGAGGAAGATCAAAATTTTGGA
GGCATATGGTAGTGGCAAGGGAAGTAATGGTCATTGATTTTTCATATTGTACTTTGGTACTTTTGGCATGAGATGCCCTTTATGTAATATGGGAACTAGTTGGCCATTTTGTACATGAGTTTTCATTATGTATTGGAGATATGATGTTGCATTTTTTAATACATGGATGAAATCAATATTTTGGAATACATGTGCATACT
TTGGACATATTGTTGAG

Intron 2 GTAATTGCAG ... AATGGTCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.23	4	15	3
A2	42.86	2	2	1
A3	66.67	1	3	1
A4	100	0	1	2
A5	50	2	3	1
A6	60	9	17	10
A7	42.65	24.3	21	15
A8	51.84	13.8	19	10.7
A9	68.99	6.9	12	18.7
A10	42.86	4	4	2

>TRINITY_DN44735_c0_g2_i2 from sample A6 / Example spliced isoform

GCGGAGAGCAAGATGGAGGAGTGTGCTATTGGATAGACTTTGACCATCCACAACGATTGTCTGGTGTTGATGCATCGACTATCAATTGATCAGTTGTTGTGGCCTCGTAGAAACTTGTGAATGGAGGGATCAACATGCCAACACCTTTGATCTTTTGAGTACCAGTGTATCTCTGATTCTCAAAGAAAATGCATCCATTA
TTATGGCCTTGAACTAGGATGCTCAATTGATGGATCCACCTTGGAACACATTGCCTATAAGATTGACTTACTCATGCATGGCAGCCTCAAACTTCCTCGAGCATAGGGAAGAGGAAGATCAAAATTTTGGAGGCATATGGTAGTGGCAAGGGAAGAGGAAGATCAAAATTTTGGAGGCATATGGTAGTGGCAAGGGAAGT
AATGGTCATTGATTTTTCATATTGTACTTTGGTACTTTTGGCATGAGATGCCCTTTATGTAATATGGGAACTAGTTGGCCATTTTGTACATGAGTTTTCATTATGTATTGGAGATATGATGTTGCATTTTTTAATACATGGATGAAATCAATATTTTGGAATACATGTGCATACTTTGGACATATCGTTGAGTG

>TRINITY_DN44735_c0_g2_i5 from sample A6 / Example unspliced isoform:

CGACGGAGAGCCCCTCGGAAGGGGAACTAAAATGACTCTGTTTCTAAAGGAGGACCAGTGTGCTATTGGATAGACTTTGACCATCCACAACGATTGTCTGGTGTTGATGCATCGACTATCAATTGATCAGTTGTTGTGGCCTCGTAGAAACTTGTGAATGGAGGGATCAACATGCCAACACCTTTGATCTTTTGAGTACC
AGTGTATCTCTGATTCTCAAAGAAAATgtaattgcagatcattactcccattgcatcattttcaaagactaattgaagtatctttaacttgtttgattagtgtgtgtttgcaatttgttatttaacaaactcttgcaaaaatggtcaagGCATCCATTATTATGGCCTTGAACTAGGATGCTCAATTGATGGATCCACCT
TGGAACACATTGCCTATAAGATTGACTTACTCATGCATGGCAGCCTCAAACTTCCTCGAGCATAGGGAAGAGGAAGATCAAAATTTTGGAGGCATATGGTAGTGGCAAGGGAAGAGGAAGATCAAAATTTTGGAGGCATATGGTAGTGGCAAGGGAAGTAATGGTCATTGATTTTTCATATTGTACTTTGGTACTTTTGG
CATGAGATGCCCTTTATGTAATATGGGAACTAGTTGGCCATTTTGTACATGAGTTTTCATTATGTATTGGAGATATGATGTTGCATTTTTTAATACATGGATGAAATCAATATTTTGGAATACATGTGCATACTTTGGACATATCGTTGAGTG