sp|Q9FMM3|EXA1_ARATH ----> Protein ESSENTIAL FOR POTEXVIRUS ACCUMULATION 1


Intron 1 GTATGTTCAT ... GTCCATCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A131.82908.242
A219.896210.615.4
A325.77728.625
A429.82484.820.4
A522.22143.44
A629.1514113.858
A723.4719330.659.2
A823.8324825.277.6
A923.9914724.646.4
A1025.1512214.441



>TRINITY_DN121285_c1_g2_i4 from sample A2 / Example spliced isoform
GGATTGATTAGCGATAACATAAAATATTAATTAGCGAAAGATCGCGAGGAAGGGAAGGGTTCCAATTTCACATCTGTTGCTATCTCCATTTCCCTTCCCTCGACTTGATAAGTAGTAAGCGCATCACCTCCCTTGGTCGATTACTTTTAATTTCAGCGACATACAGCCCGATCTCTCTGCGCGCACGGACTTAAGACCCC
AGCCTTGGTATATCCCGCACTTGTTTCGCATTTGCCCTTGCTGCTGCCACTGCTTCAACCGCAAATCCCTGTCGTATCCTCGCCCTCCTTTACGAGCCATGGGTTTCATCCTTCCGAAACCCCATATGGTTTGAATTTGCATACCCCGTCCTGTTCTTTAGTAATTTCTTCCCGTACATTCGATTTTCAGGGCACAATCT
GCAAGAGTAGCGAACCTAACGAGGTAGTCGTTAAATACCCCTTGGATTTGGTTTTTGTTCGAGGCGTAAGAATGGCCGAAAGGAAGTCCGATCTTCCGCAGCATTCTGAGACGCAGGATCATCCGAATATCAAAGTGTTGTGCTTAGATGGAGACAGCTCGGGATTGTTGTCTGGTAACCCTGATGCGGAATTTAAAGAT
GTATCTGGGCCTGAAAATGTAATCCCTCTTTCTCCTCAATGGCTGCACTCAAGGGTTGGTGAGACCAAGGAACCGCGGACTCCAAACAGTTTTAGTCAGAGTGGAAGCACTGATGCAAATCTCAAAGAGCAATGGAGAGGAGAGGGTTCTCTTGACAAGAAAGATTGGCGGAGAACTGCTCCTGGTGAGAGCGACGGTGG
TCGGCGCTGGCGTGAAGAGGAAAGGGAAAATACTGCAGTTGCCAGAAGAGACCGTAGAAAAGAAGGGGACAGAGAAGTTGAAACTCGGAAGGTTGATCGTAGATCAGAGAATACTTCACTAAGAGAAGTTGCAGACAATAAGCATCCAAATTCGTCAGACCGTTGGCAAGATACTACTGCCCGTGATCCAGGCCACGAGG
CACGTCGTGACAGGAAATGGTCCACAGCATGGGGACCTGAAAACAATGAAAAAGATATACGTCGAGAGAAACGCAGTGAAGTGGACAAGGAGGATGGTGGTCTTGATAGACAACCTTCTTTTTCAAGCAGTCGTCTATTGGCTGCAGACCGTGAATCGGAAAAATCATCGACTGACAAGTGGAGACCATCCAGACTCCGG
GACAAACTTAAAACACAAAAGGATGGTGTTGGATTCCTTGCAGGTCGAGGCCGTGGAAATGGTTTGACAAATAGTCTTTCGATGAGAACTCCTATTGGTGCTTCTACTATTACAAATGCTTTTTGCTACCCACGGGCCAAACTTCTTGACATTTATAGGGATTGTGGATCTTTCTCACCTTTACGAGGTGTTGCCGATGG
ATTTGAGGAAGCTTCTGGCGTTACTTCTTCTGACCATTTTGAGCCATTGGCATTTGTCTCGCCTGAGCCAGAAGAGCAGGCTGTATTAGAAGACATTTCTAAGGGGAAAATTGCAGGCGGTGAAGCAATCCGCAGGACATTTAACAATACATCTGGCAGGATCAATATTGATGGCCTTGGAATTAGAGAGGAGATATTGT
CCAAGAGCAATAAGAAAAGGGATAGTGATGAATCTGTGAGCTGTGTGATGGATGATTCTCAATTAAAGGTGCATGAAGATGTGCTCCCTGATTTAGATGAGTCTGAACTTGTAGATTCAGTTTCCAAGGAGATCACAGCAGGCAGCAAAGATAAGACAATGTATTCAGTTCCCACATTATATGATTTCAATGATGAGATA
TTCATCCCAAAGGCTTCTGCTGAGGCAAGGAGGGCTGCCAAATCTTTTGAAGGAATATCTGAATATCCTTTCGTTGAAGAGATGATGGGTGAGGAGGTTTGTCATGAAAATGAAAATGTAAATTCATCAGGACTAGGTAATTCTTATGATAATCTATCCATGAAAGGAGAAGCATCAATGGCAGTTGGGGAGAGGAAGAG
TACAAAAGATATTGAAGTGACTTCTTCTGAGCTGCCTATCGGGCTACCATCTGATGCAAGTGTGCTTTTTGAAGACAAATCCTTTGAAGGGACTCCTGTAGAGTGTGATTTCGAGGATGGAAAATCAGGAAATAAGGATGGCATCAAGCTAGGAGAACAAGGTTTCCTGCTTGAGGAACTAAGTCTTTTTTATAAGGATC
CTCAAGGGGATATTCAGGGGCCTTTTCTAGGTATAGATATAATTCAATGGTTTGAACAAGGTTTTTTTGGTACAGATTTACTAGTGTGCCTTTCTGATGCTCCGGAGGGAACACCATTTCAGCCCCTTGGAGAGCTTATTCTACACATGAGACATAAAGCTCAATCATCTCATGGTACTGTCTTTGAGGATTCAGAAGAC
ACCAAAGGAATGAGTTCAAGAAGTGGTCATGATATTTCATCTAAAAAGGCTGGTATCATATATGAACATAATTCAATACTCAATCCTGAGCAAGAAAAGACATTGTCCGACTTTTCAGAGCAGGTCAATGCAGGATTAACTGGCAGAGGACTATCTTTTCCTGATATGCTCAAAGGTACTAAGAATAATACACTTGATCA
TCAAGAACAACACTTTTTGGAATCTGCTACAGAGAAAAATGCAGAAATTCTTGCAAGAATAATTCATCATGGAATACCACCGGGAGAATCTATTGGAAGTCTTGAGAGGCAGAGAATGTCTTCCTTACCTCGGTTTGATACAGATGATGGCCTTGTTCCTTGGCAATCTGAAGCAAATGCTTTCGGTTCAGTCTGGCCTC
AGCAGGAAGCGGATCAGTTCATTGATATAAGATCCGGTCTGCTGGATGCCACAGTTAGCAGTTCCATTTCTGGTAATATGCTTTCAATGATGTCAAGGATGCAATCCCTACCTAATAATCAGCGACAAGACTTATTTCATCAATCAAATGGTGCCGGCACTTCTCCTCCAATTGATAGGGCTTGGTCTGAAACATTTCAG
AGGGATGCATATTCCACTAACAAAATTTTCAATGTTGCATCTGATCCTCATCGTATGACACATCCTGAACAGAATTTGCAAAGAATAGAGCGAGCTAACCTTTTATTTCATCACCAGTTGGAGCAACAGAAGCAGAAACAGCAACAGCAGCAGCAACAACAACAACAAATGCTGATGCAACGGCAATTGCAGTCCGGAGC
TAAATTGCTGGATCACAGTACAATTTCAGGTATGGGTCATAACTTTGACTCTTCACTGTTGCAACACCACCAATTGCTACAACCACCTTTACAACCACGTCAGCAACTACCTCCATCTGCGCATGTTATGGACCCAATCTCAAGGCTTCAGCTGCAGCATCACCATCACCAACCCCAACACCATCTACAACTACAACCAC
ATCAGCAGCAAACCCAGCATAATATGCAGATGGAGCACCAACGGCAGTCTCATCAAACAGGCCTAGATCAGTTACTGTCTCATCAGATATATGATCCTAGCTATGCTCAGAATCAATTTCCTTCTGGTGGAAGTAGCATTGATCAGCTTATGTTAAGGCAAGAAGGTATTAGGCATGAATCCCAGCCACATCATCAGTCT
ATACGTAGGCAAAGCAATTCAATGGACCCAAGCCTTTCATTGGAACAACTTTTGCATCAGAGAAGACATGAGCTATCAGCTAACCAGTTTCCCTTGCCATCGCAACGGCAATTTATTGATGAAAGACGTGTAAGTGGAGTTTGGGCTGTTGATGAGTCTGGTGAGTTTGTACAGACGCAAGCACCTACTCTCCATCCCTT
TGATTTATACCAGCCGCATCAGCAGATACTGAATTCACAACCACAACACACACAATTATTAAATTTTCAATCTTCAAGCACCCTAGCATCTAATGCAGAAATAAAATATCCTTACCTATTTGAGCGGGCTGAGCATCATGACCATAGCAGTGAAGGTCGATCATTGCATGGTATGCATGATCCCAACCTGTTACCTTTTG
AAAGGCCTACTGATTTGTCTTCGCTGAACATGGACTTCTCACATTCTCTTAAACAAGCCCAGGAACGCAGGGTGCAGGAAAGGTATAATGTTACACGTTTGGCAGGACAGGATGAACAATTCCCATCAGAAGTTACCTACAATTCCCGATCTCATCCAATGGCCTTCCATGGTATACAATCTGAGGTTACAAATGGGATT
TGGGAGCAACCCTCCAGCACACATTCTACTGATCTTCATGTTCAGGGTCCTGGCTTTTTTATGCAGCAAGGACAACCTTTCGATTTTATTACTGAAAGCCAAGGTCAGAAGTTGCATCGAGTTGGGTTAAATTGGCAGTCCAATGAAGGTTCTCAAGAGCATGTTTCTTCTGAAGAATTGCACCGGAGATCTTTAATTTC
TACACACACTGCTGATTTAGAGACAAGACAATCTCATGGCGACTCGCTTCATCAAAAATTCCCTCTTCGTTCTGGTTCGTCATCTGTAATGCCTGGTAATGTGCTTGCTACTTCTTCTGAACAAAGCCAGTGTATGTGGACTGCTGTGAACTCAAGAGAGTGTATTTCTGGTTGTACTTATGATTCTTCTGATTTGATTT
CAAGCCAGCTATTCAAAAATCGTAGAAATGAAGATCTTTCAGAGGTTCTAGAAGATTTGGCTGGGAATGGTGTTGGATCGACTTATATGGCCTGTAACTCAGCATCTGAAATGCAGATGGGGACTGGCTTATCTGTAGATAGTGTATATGATGGTATGCATTCAGAAGCATTTATTGAGGAGCCATTTTATTCATCAAAT
AACAATGAGCTGCAACTGTCTCTGCAAGTGTCTGATTCACTCGGGCAATCCTCACGAGATGACATTGAGAGTTTGGAATTCAAAGAAGCTAAAAAAAACAAACGGCGGAGTTCTAAAGCTAGAGGGACAGTAAAAATGACTGTTGTTTCTTCAGATGCGTCTTCAGTGTCAAACTTATCTACAGGAGCTCCAGAATTGAG
TCAAAATCAACAAACAATAAAGCATGACAGTTCCACTGTGCAAGAGTTGTCTTCATGGAGTACAGATTTTAATAGACGAGGGTTATCAGTGCATACTGGCATGGACACTAAAAAATCCCTACAGAAAACTGGGCAGTTGGTTATTGAAAGCAACAATGCAGATGGGAGAATGAGTCATTCCAGTGACAATGGTGATCAAA
TATCAACAGTTGAACGGGAAAATTCTAAATTTTCTGCAAAAGCTTTTGAATTGTCAAATTTGGCTTCTTCTCCCTTATCTACAGTACAGAGAAAGCTACTGAGTAACAGAAGAGCATCTTATGACGCTGGAGAAAGCATTGCAGAAAATGCTGGTGGCAATCTTGCAGTGAAGCCTTCAGAAAGTGCTTTCCAACGGAAA
GAAAATTTACCCATTTCAAACTTTAGTAAGACTTCTGCTTCCAGTGATGCTGACACAAGGAATGAAGGAGCAAAATCCTTTCTAAATACATTGAAAAATCCAAAGAAACCAGAACCAGAAAAAGAGAAGAGCAAAGTTGATTCATCTGCAATTTTAGATTTTGATAGTCAAGGATCAAAAAGTTCAAAAAAGAAGGGCAA
GAAAGGAAGGCAGATAGACCCTTCTTTGCTTGGATTTAAGGTTACAAGTAATCGCATCAATATGGGGGAGATTCAACACCCTGATGATTAAATTCCTCTAGTTGATTTGTATTCTTAGAGTGGTTATCATAATCCAAGGTAAATATGCCATCATTGATTTGGTTTTTAAATGGGTCATATTCTTAAATTTTTTCTCTGTA
CAGGTATTTTTGGCATAGTTCCCCCCCTGATAGTTTTTTTTCCACTTATTATGAAATTGAATGATTCGAGTCTTTATCAAATGAATTTTCATTTTTTGCACCCAGTAATTGTTTTCATGTCATTTGACAATGTTTTAGGGAAGACCGGAAATCTACCTTTTGTTTGAGGTGCAATTCTATTCTCTGTTGGTCTGTTCCTC
TACTAACAAATTAAAGCCATTTATGCTTGTCACAGTACCTCTTGGTCCTTGCCATTGTGATCATGTTGTTATAGTAGGATTTGATTTATGTAAGTGGTTGAGTCGGTACTATTTACAAAATATTATAATTAAGTAGAATCTCAACATGTGGCTTTTGTTAATTTTAGTACCTTTTGTTGTCAATTTATGGGACCTGTATA
TCTATGTTAGATGGAAAAATGTTTATCGTACAATTTTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTTGATGATCTATGTTAGATGGAAAAATGTTTATCGTACAATTTTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTCGATGATAACTGTCCAGATTAAATGACATGAGTCACATTATGCAGTAATT
TCATGAGCTTTGGCTTTAGATGTTGTGACAGAGGAGTATCTAGTGTCAAAGCTGTCCACCCGGTAATTTTACCTGTCACATCTGCATCTTGCCTAATTTGCATGTCTGGTTAGTTACCTAAAGGGTTGAGATCATGGGCTAAGTAAAATAATGTGTCCACATGGGCCATACACTAGAAACCTATATGTTGCCCTGGCATA
CTTAATGGAATAAGTTAGATGCAGCAGTAGTTGCTGCAGGACCTATGACATTGAAACCATAGACTTCCCGAAAGAGGACCAAGTCAACATACATTGATCAATTCTGTATCCAAAACTCCAGTTACTACATTCTATGTGTTATTTCCTATGTTGCATGGGTACCCGTACCGGGGGGTCGGGTACACGTACCCGTACCGGGT
ACTTGGGTACTTCAATGGTATGTTCCCAGCCGTACCCAAATTCAAACTCGCGTACCAGGTACTTCACTTATGAAAACAGGG


>TRINITY_DN121285_c1_g2_i2 from sample A2 / Example unspliced isoform:
GAGTAACAGAAGAGCATCTTATGgtatgttcatcacttcacaaggctgatattgtaaatgagaatcacagtctaataaaaactgcctctaattagcaagttgtatctattgtccatccagACGCTGGAGAAAGCATTGCAGAAAATGCTGGTGGCAATCTTGCAGTGAAGCCTTCAGAAAGTGCTTTCCAACGGAAAGAA
AATTTACCCATTTCAAACTTTAGTAAGACTTCTGCTTCCAGTGATGCTGACACAAGGAATGAAGGAGCAAAATCCTTTCTAAATACATTGAAAAATCCAAAGAAACCAGAACCAGAAAAAGAGAAGAGCAAAGTTGATTCATCTGCAATTTTAGATTTTGATAGTCAAGGATCAAAAAGTTCAAAAAAGAAGGGCAAGAA
AGGAAGGCAGATAGACCCTTCTTTGCTTGGATTTAAGGTTACAAGTAATCGCATCAATATGGGGGAGATTCAACACCCTGATGATTAAATTCCTCTAGTTGATTTGTATTCTTAGAGTGGTTATCATAATCCAAGGTAAATATGCCATCATTGATTTGGTTTTTAAATGGGTCATATTCTTAAATTTTTTCTCTGTACAG
GTATTTTTGGCATAGTTCCCCCCCTGATAGTTTTTTTTCCACTTATTATGAAATTGAATGATTCGAGTCTTTATCAAATGAATTTTCATTTTTTGCACCCAGTAATTGTTTTCATGTCATTTGACAATGTTTTAGGGAAGACCGGAAATCTACCTTTTGTTTGAGGTGCAATTCTATTCTCTGTTGGTCTGTTCCTCTAC
TAACAAATTAAAGCCATTTATGCTTGTCACAGTACCTCTTGGTCCTTGCCATTGTGATCATGTTGTTATAGTAGGATTTGATTTATGTAAGTGGTTGAGTCGGTACTATTTACAAAATATTATAATTAAGTAGAATCTCAACATGTGGCTTTTGTTAATTTTAGTACCTTTTGTTGTCAATTTATGGGACCTGTATATCT
ATGTTAGATGGAAAAATGTTTATCGTACAATTTTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTTGATGATCTATGTTAGATGGAAAAATGTTTATCGTACAATTTTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTCGATGATAACTGTCCAGATTAAATGACATGAGTCACATTATGCAGTAATTTCA
TGAGCTTTGGCTTTAGATGTTGTGACAGAGGAGTATCTAGTGTCAAAGCTGTCCACCCGGTAATTTTACCTGTCACATCTGCATCTTGCCTAATTTGCATGTCTGGTTAGTTACCTAAAGGGTTGAGATCATGGGCTAAGTAAAATAATGTGTCCACATGGGCCATACACTAGAAACCTATATGTTGCCCTGGCATACTT
AATGGAATAAGTTAGATGCAGCAGTAGTTGCTGCAGGACCTATGACATTGAAACCATAGACTTCCCGAAAGAGGACCAAGTCAACATACATTGATCAATTCTGTATCCAAAACTCCAGTTACTACATTCTATGTGTTATTTCCTATGTTGCATGGGTACCCGTACCGGGGGGTCGGGTACACGTACCCGTAC



Intron 2 GTAGGTTCCT ... TACTATGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A112.2108155
A27.597360
A318.11104230
A414.7481140
A519.352560
A640640
A722.582470
A8162140
A911.433140
A1014.814680



>TRINITY_DN125232_c0_g3_i6 from sample A1 / Example spliced isoform
GATTAGCGATAACATAAAATATTAATTAGCGAAAGATCGCGAGGAAGGGAAGGGTTCCAATTTCACATCTGTTGCTATCTCCATTTCCCTTCCCCCGACTTGATAAGTAGTAAGCGCATCACCTCCCTTGGTCGATTACTTTTAATTTCAGCGACATACAGCCCGATCTCTCTGCGCGCACGGACTTAAGACCCCAGCCT
TGGTATATCCCGCACTTGTTTCGCATTTGCCCTTGCTGCTGCCACTGCTTCAACTGCAAATCCCTGTCGTATCCTCGCCCTCCTTTACGAGCCATGGGTTTCATCCTTCCGAAACCCCATATGGTTTGAATTTGCATACCCCCTCCTGTTCTTCAGTAATTTCTTCCCGTACATTCGATTTTCAGGGCACAATCTGCAAG
AGTAGCGAACCAGAGGTAGTCGTTAAATACCCCTTGGATTTGGTTTTTGTTCGAGGCGTAAGAATGGCCGAAAGGAAGTCCGATCTTCCGCAGCATTCTGAGACGCAGGATCATCCGAATATCAAAGTGTTGTGCTTAGATGGAGACAGCTCGGGATTGTTGTCTGGTAACCCTGATGCGGAATTTAAAGATGTATCTGG
GCCTGAAAATGTAATCCCTCTTTCTCCTCAATGGCTGCACTCAAGGGTTGGTGAGACCAAGGAACCGCGGACTCCAAACAGTTTTAGTCAGAGTGGAAGCACTGATGCAAATCTCAAAGAGCAATGGAGAGGAGAGGGTTCTCTTGACAAGAAAGATTGGCGGAGAACTGCTCCTGGTGAGAGCGACGGTGGTCGGCGCT
GGCGTGAAGAGGAAAGGGAAAATACTGCAGTTGCCAGAAGAGACCGTAGAAAAGAAGGGGACAGAGAAGTTGAAACTCGGAAGGTTGATCGTAGATCAGAGAATACTTCACTAAGAGAAGTTGCAGACAATAAGCATCCAAATTCGTCAGACCGTTGGCAAGATACTACTGCCCGTGATCCAGGCCACGAGGCACGTCGT
GACAGGAAATGGTCCACAGCATGGGGACCTGAAAACAATGAAAAAGATATACGTCGAGAGAAACGCAGTGAAGTGGACAAGGAGGATGGTGGTCTTGATAGACAACCTTCTTTTTCAAGCAGTCGTCTATTGGCTGCAGACCGTGAATCGGAAAAATCATCGACTGACAAGTGGAGACCATCCAGACTCCGGGACAAACT
TAAAACACAAAAGGATGGTGTTGGATTCCTTGCAGGTCGAGGCCGTGGAAATGGTTTGACAAATAGTCTTTCGATGAGAACTCCTATTGGTGCTTCTACTATTACAAATGCTTTTTGCTACCCACGGGCCAAACTTCTTGACATTTATAGGGATTGTGGATCTTTCTCATCTTTACGAGGTGTTGCCGATGGATTTGTGG
AAGTTTCTGGTGTTACTTCTTCTGACCATTTTGAGCCATTGGCATTTGTCTCGCCTGAGCCAGAAGAGCAGGCTGTATTAGAAGACATCTCTAAGGGGAAAATTGCAGGCGGTGAAGCAATCCGCAGGACATTTAACAATACATCTGGCAGGATCAATATTGATGGCCTTGGAATTAGTGAGGAGATATTGTCCAAAAGC
AATAAGAAAAGGGATAGTGATGAATCTGTGAGCTGTGTGATGGATGATTCTCAATTAAAGGTGCATGAAGATGTGCTCCCTGATTTAGATGAGTCTGAACTTGTAGATTCAGTTTCCAAGGAGATCACAGCAGGCAGCAAAGATAAGACAATGTATTCAGTTCCCACATTATATGATTTCAATGATGAGATATTCATCCC
AAAGGCTTCTGCTGAGGCAAGGAGGGCTGCCAAATCTTTTGAAGGAATATCTGAATATCCTTTCGTTGAAGAGATGATGGGTGAGGAGGTTTGTCATGAAAATGAAAATGTAAATTCATCAGGACTAGGTAATTCTTATGATAATCTATCCATGAAAGGAGAAGCATCAATGGCAGTTGGGGAGAGGAAGAGTACAAAAG
ATATTGAAGTGACTTCTTCTGAGCTGCCTATCGGGCTACCATCTGATGCAAGTGTGCTTTTTGAAGACAAATCCTTTGAAGGGACTCCTGTAGAGTGTGATTTCGAGGATGGAAAATCAGGAAATAAGGATGGCATCAAGCTAGGAGAACAAGGTTTCCTGCTTGAGGAACTAAGTCTTTTTTATAAGGATCCTCAAGGG
GATATTCAGGGGCCTTTTCTAGGTATAGATATAATTCAATGGTTTGAACAAGGTTTTTTTGGTACAGATTTACTAGTTTGCCTTTCTGATGCTCCGGAGGGAACACCATTTCAGCCCCTTGGAGAGCTTATTCTACACATGAGACATAAAGCTCAATCATCTCATGGTACTGTCTTTGAGGATTCAGAAGACACCAAAGG
AATGAGTTCAAGAAGTGGTCATGATATTTCATCTAAAAAGGCTGGTATCATATATGAACATAATTCAATACTCAATCCTGAGCAAGAAAAGACATTGTCCGACTTTTCAGAGCAGGTCAATGCAGGATTAACTGGCAGAGGACTATCTTTTCCTGATATGCTCAAAGGTACTAAGAATAATACACTTGATCATCAAGAAC
AACACTTTTTGGAATCTGCTACAGAGAAAAATGCAGAAATTCTTGCAAGAATAATTCATCATGGAATACCACCGGGAGAATCTATTGGAAGTCTTGAGAGGCAGAGAATGTCTTCCTTACCTCGGTTTGATACAGATGATGGCCTTGTTCCTTGGCAATCTGAAGCAAATGCTTTTGGTTCAGTCTGGCCTCAGCAGGAA
GCGGATCAGTTCATTGATATAAGATCCGGTCTGCTGGATGCCACAGTTAGCAGTTCCATTTCTGGTAATATGCTTTCAATGATGTCAAGGATGCAATCCCTACCTAATAATCAGCGACAAGACGTATTTCATCAATCAAATGGTGCCGGCACTTCTCCTCCAATTGATAGGGCTTGGTCTGAAACATTTCAGAGGGATGC
ATATTCCACTAACAAAATTTTCAATGTTGCATCTGATCCTCATCGTATGACACATCCTGAACAGAATTTGCAAAGAATAGAGCGAGCTAACCTTTTATTTCATCACCAGTTGGAGCAACAGAAGCAGAAACAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAACAACAACAACAAATGCTGATGCAACGGCAATTGCAGT
CCGGAGCTAAATTGCTGGATCACAGTACAATTTCAGGTATGGGTCATAACTTTGACTCTTCACTGTTGCAACACCACCAATTGCTACAACCACCTTTACAACCACGTCAGCAACTACCTCCATCTGCGCATGTTATGGACCCAATCTCAAGGCTTCAGCTGCAGCATCACCATCACCAACCCCAACACCATCTACAACTA
CAACCACATCAGCAGCAAACCCAGCATAATATGCAGATGGAGCACCAACGGCAGTCTCATCAAACAGGCCTAGATCAGTTACTGTCTCATCAGATATATGATCCTAGCTATGCTCAGAATCAATTTCCTTCTGGTGGAAGTAGCATTGATCAGCTTATGTTAAGGCAAGAAGGTATTAGGCATGAATCCCAGCCACATCA
TCAGTCTATACGTAGGCAAAGCAATTCAATGGACCCAAGCCTTTCATTGGAACAACTTTTGCATCAGAGAAGACATGAGCTATCAGCTAACCAGTTTCCCTTGCCATCGCAACGGCAATTTATTGATGAAAGACGTGTAAGTGGAGTTTGGGCTGTTGATGAGTCTGGTGAGTTTGTACAGACGCAAGCACCTACTCTCC
ATCCCTTTGATTTATACCAGCCGCATCAGCAGATACTGAATTCACAACCACAACACACACAATTATTAAATTTTCAATCTTCAAGCACCCTAGCATCTAATTCAGAAATAAAATATCCTTACCTATTTGAGCGGGCTGAGCATCATGACCATAGCAGTGAAGGTCGATCATTGCATGGTATGCATGATCCCAACCTGTTA
CCTTTTGAAAGGCCTACTGATTTGTCTTCGCTGAACATGGACTTCTCACATTCTCTTAAACAAGCCCAGGAACGCAGGGTGCAGGAAAGGTATAATGTTACACGTTTGGCAGGACAGGATGAACAATTCCCATCAGAAGTTACCTACAATTCCCGATCTCATCCAATGGCCTTCCATGGTATACAATCTGAGGTTACAAA
TGGGATTTGGGAGCAACCCTCCAGCACACATTCTACTGATCTTCATGTTCAGGGTCCTGGCTTTTTTATGCAGCAAGGACAACCTTTCGATTTTATTACTGAAAGCCAAGGTCAGAAGTTGCATCGAGTTGGGTTAAATTGGCAGTCCAATGAAGGTTCTCAAGAGCATGTTTCTTCTGAAGAATTGCACCGGAGATCTT
TAATTTCTACACACACTGCTGATTTAGAGACAAGACAATCTCATGGCGACTCGCTTCATCAAAAATTCCCTCTTCGTTCTGGTTCGTCATCTGTAATGCCTGGTAATGTGCTTGCTACTTCTTCTGAACAAAGCCAGTGTATGTGGACTGCTGTGAACTCAAGAGAGTGTATTTCTGGTTGTACTTATGATTCTTCTGAT
TTGATTTCAAGCCAGCTATTCAAAAATCGTAGAAATGAAGATCTTTCAGAGGTTCTAGAAGATTTGGCTGGGAATGGTGTTGGATCGACTTATATGGCCTGTAACTCAGCATCTGAAATGCAGATGGGGACTGGCTTATCTGTAGATAGTGTATATGATGGTATGCATTCAGAAGCATTTATTGAGGAGCCATTTTATTC
ATCAAATAACAATGAGCTGCAACTGTCTCTGCAAGTGTCTGATTCACTCGGGCAATCCTCACGAGATGACATTGAGAGTTTGGAATTCAAAGAAGCTAAAAAAAACAAACGGCGGAGTTCTAAAGCTAGAGGGACAGTAAAAATGACTGTTGTTTCTTCAGATGCGTCTTCAGTGTCAAACTTATCTACAGGAGCTCCAG
AATTGAGTCAAAATCAACAAACAATAAAGCATGACAGTTCCACTGTGCAAGAGTTGTCTTCATGGAGTACAGATTTTAATAGACGAGGGTTATCAGTGCATACTGGCATGGACACTAAAAAATCCCTACAGAAAACTGGGCAGTTGGTTATTGAAAGCAACAATGCAGATGGGAGAATGAGTCATTCCAGTGACAATGGT
GATCAAATATCAACAGTTGAACGGGAAAATTCTAAATTTTCTGCAAAAGCTTTTGAATTGTCAAATTTGGCTTCTTCTCCCTTATCTACAGTACAGAGAAAGCTACTGAGTAACAGAAGAGCATCTTATGACGCTGGAGAAAGCATTGCAGAAAATGCTGGTGGCAATCTTGCAGTGAAGCCTTCAGAAAGTGCTTTCCA
ACGGAAAGAAAATTTACCCATTTCAAACTTTAGTAAGACTTCTGCTTCCAGTGATGCTGACACAAGGAATGAAGGAGCAAAATCCTTTCTAAATACATTGAAAAATCCAAAGAAACCAGAACCAGAAAAAGAGAAGAGCAAAGTTGATTCATCTGCAATTTTAGATTTTGATAGTCAAGGATCAAAAAGTTCAAAAAAGA
AGGGCAAGAAAGGAAGGCAGATAGACCCTTCTTTGCTTGGATTTAAGGTTACAAGTAATCGCATCAATATGGGGGAGATTCAACACCCTGATGATTAAATTCCTCTAGTTGATTTGTATTCTTAGAGTGGTTATCATAATCCAAGGTAAATATGCCATCATTGATTTGGTTTTTAAATGGGTCATATTCTTAAATTTTTT
CTCTGTACAGGTATTTTTGGCATAGTTCCCCCCCTGATAGTTTTTTTTCCACTTATTATGAAATTGAATGATTCGAGTCTCTATCAAATGAATTTTCATTTGTTGCACCCAGTAATTGTTTTCATGTCATTTTACAATGTTTTAGGGAAGACCGGAAATCTACCTTTTGTTTGAGGTGCAATTC


>TRINITY_DN125232_c0_g3_i8 from sample A1 / Example unspliced isoform:
GATTAGCGATAACATAAAATATTAATTAGCGAAAGATCGCGAGGAAGGGAAGGGTTCCAATTTCACATCTGTTGCTATCTCCATTTCCCTTCCCCCGACTTGATAAGTAGTAAGCGCATCACCTCCCTTGGTCGATTACTTTTAATTTCAGCGACATACAGCCCGATCTCTCTGCGCGCACGGACTTAAGACCCCAGCCT
TGGTATATCCCGCACTTGTTTCGCATTTGCCCTTGCTGCTGCCACTGCTTCAACTGCAAATCCCTGTCGTATCCTCGCCCTCCTTTACGAGCCATGGGTTTCATCCTTCCGAAACCCCATATGGTTTGAATTTGCATACCCCCTCCTGTTCTTCAGTAATTTCTTCCCGTACATTCGATTTTCAGGGCACAATCTGCAAG
AGTAGCGAACCAGAGGTAGTCGTTAAATACCCCTTGGATTTGGTTTTTGTTCGAGGCGTAAGAATGGCCGAAAGGAAGTCCGATCTTCCGCAGCATTCTGAGACGCAGGATCATCCGAATATCAAAGTGTTGTGCTTAGATGGAGACAGCTCGGGATTGTTGTCTGGTAACCCTGATGCGGAATTTAAAGATGTATCTGG
GCCTGAAAATGTAATCCCTCTTTCTCCTCAATGGCTGCACTCAAGGGTTGGTGAGACCAAGGAACCGCGGACTCCAAACAGTTTTAGTCAGAGTGGAAGCACTGATGCAAATCTCAAAGAGCAATGGAGAGGAGAGGGTTCTCTTGACAAGAAAGATTGGCGGAGAACTGCTCCTGGTGAGAGCGACGGTGGTCGGCGCT
GGCGTGAAGAGGAAAGGGAAAATACTGCAGTTGCCAGAAGAGACCGTAGAAAAGAAGGGGACAGAGAAGTTGAAACTCGGAAGGTTGATCGTAGATCAGAGAATACTTCACTAAGAGAAGTTGCAGACAATAAGCATCCAAATTCGTCAGACCGTTGGCAAGATACTACTGCCCGTGATCCAGGCCACGAGGCACGTCGT
GACAGGAAATGGTCCACAGCATGGGGACCTGAAAACAATGAAAAAGATATACGTCGAGAGAAACGCAGTGAAGTGGACAAGGAGGATGGTGGTCTTGATAGACAACCTTCTTTTTCAAGCAGTCGTCTATTGGCTGCAGACCGTGAATCGGAAAAATCATCGACTGACAAGTGGAGACCATCCAGACTCCGGGACAAACT
TAAAACACAAAAGGATGGTGTTGGATTCCTTGCAGGTCGAGGCCGTGGAAATGGTTTGACAAATAGTCTTTCGATGAGAACTCCTATTGGTGCTTCTACTATTACAAATGCTTTTTGCTACCCACGGGCCAAACTTCTTGACATTTATAGGGATTGTGGATCTTTCTCATCTTTACGAGGTGTTGCCGATGGATTTGTGG
AAGTTTCTGGTGTTACTTCTTCTGACCATTTTGAGCCATTGGCATTTGTCTCGCCTGAGCCAGAAGAGCAGGCTGTATTAGAAGACATCTCTAAGGGGAAAATTGCAGGCGGTGAAGCAATCCGCAGGACATTTAACAATACATCTGGCAGGATCAATATTGATGGCCTTGGAATTAGTGAGGAGATATTGTCCAAAAGC
AATAAGAAAAGGGATAGTGATGAATCTGTGAGCTGTGTGATGGATGATTCTCAATTAAAGGTGCATGAAGATGTGCTCCCTGATTTAGATGAGTCTGAACTTGTAGATTCAGTTTCCAAGGAGATCACAGCAGGCAGCAAAGATAAGACAATGTATTCAGTTCCCACATTATATGATTTCAATGATGAGATATTCATCCC
AAAGGCTTCTGCTGAGGCAAGGAGGGCTGCCAAATCTTTTGAAGGAATATCTGAATATCCTTTCGTTGAAGAGATGATGGGTGAGGAGGTTTGTCATGAAAATGAAAATGTAAATTCATCAGGACTAGGTAATTCTTATGATAATCTATCCATGAAAGGAGAAGCATCAATGGCAGTTGGGGAGAGGAAGAGTACAAAAG
ATATTGAAGTGACTTCTTCTGAGCTGCCTATCGGGCTACCATCTGATGCAAGTGTGCTTTTTGAAGACAAATCCTTTGAAGGGACTCCTGTAGAGTGTGATTTCGAGGATGGAAAATCAGGAAATAAGGATGGCATCAAGCTAGGAGAACAAGGTTTCCTGCTTGAGGAACTAAGTCTTTTTTATAAGGATCCTCAAGGG
GATATTCAGGGGCCTTTTCTAGGTATAGATATAATTCAATGGTTTGAACAAGGTTTTTTTGGTACAGATTTACTAGTTTGCCTTTCTGATGCTCCGGAGGGAACACCATTTCAGCCCCTTGGAGAGCTTATTCTACACATGAGACATAAAGCTCAATCATCTCATGGTACTGTCTTTGAGGATTCAGAAGACACCAAAGG
AATGAGTTCAAGAAGTGGTCATGATATTTCATCTAAAAAGGCTGGTATCATATATGAACATAATTCAATACTCAATCCTGAGCAAGAAAAGACATTGTCCGACTTTTCAGAGCAGGTCAATGCAGGATTAACTGGCAGAGGACTATCTTTTCCTGATATGCTCAAAGGTACTAAGAATAATACACTTGATCATCAAGAAC
AACACTTTTTGGAATCTGCTACAGAGAAAAATGCAGgtaggttcctcaacactcttcttctaggaaatgatttaaatgtgagttttaacatgttatttttatgtcacagttctactaatctattattctttactatgcagAAATTCTTGCAAGAATA



Intron 3 GTGAGGTAGC ... TGTACTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A177.4271929
A261.17203726
A371.43122634
A485.7131224
A585.9642128
A672.4141110
A765.71121927
A853.859516
A970.67111835
A1068.81173837



>TRINITY_DN43839_c1_g3_i13 from sample A9 / Example spliced isoform
AGGTGAGGTAGCAGCTGATGAAGCCAGTAAATTGGATGGTGATATAGCTGTTAGCGGTAAGAGCAGGGTTGCTTCTTTCAGTTGTCTGAATGAATTAAATATATCCTCTGCTATTGTGCTTGATAAAACTCTTATTGAAGAGCTATTGATTGGGAGAACAGGAGATGATGAAAATTCTCTAAAATATAAAGAAGAGACTT
CCAAAAGTCAGCAAGTAGTGGGAAGGAGTTCTTGTCAAGAAACGTCAACAAGTGTAGTTCATTGTAGGCCAGTAGAAGGTCTGGGGGAACATGACTATGCTGTTTATACCAATGCCATTTCATGTGGGGGTGTTCCACTCTCTGACAATAAGGAAGAACCAATTGACAGCTCAGAAGATGTGAGCTCAAGTTTAGAGGGT
AGTGAGCCTATTTATGAAGGGGAGGAATGCATTCTAGTGGATCCTGCACCTCAGATGTATGAAAGTCGAGAGCCTTTATATGAAGGTGAAATGGTTTTAGCAGAGCAGGCTGACAAAGGAGTAATTTTACAAAGTATAGGGTTGGGATCTGGAGATGAAATTTCAATTCAAGAACAGGAAGAATCTCTGAGTGCAGCAGA
TAGAGAAGGAACACTGATTCGGAATTTTTTGAGGAGTAATGCCAGTCAATTGACAGTTTTTGGGCTTTTTTGTCTGCAAGAAGGATTGAAGGACTGCGAACTCTGTGTTTTTTTCCGTAACAATCACTTTAGTACAATGTTTAAGTTCAAGGGGGATCTCTACCTCCTAGCAACAGATCAAGGTTATATAAATCAGCCTG
ATTTGGTGTGGGAAAAACTTAATGAGGTCCATGGAGATTCTGTGTTCATGACTGGGGACTTCAAGGAGTTCAAAGCAGAAGATCAAGTTGGCAGCTCATGGAATCAACATCATGCTGTGGCTAAAACTGCAGATTATATTGCCTCTGTGACCAATACGCAGCCGTCAGATGCAAATTTCAATTCAGATTTGCAACTTGCA
ATTGCTCTTCAGCAACAAGAATTTGAGCAGCAGCAGCAGCAGCAGCAACAACAACAACAACAACAACATTTGCAACACTATCCGCGTGCTACTCAGTCATCAGTGCCTGCTAATTCCAAGATGATTACAGGTCCAGAGGCACCCAAGGCCAGCTCCTCTACGTCTAAGCAGGAAGCAAGGAAGGAGAGGTGTGTTGTAAT
GTGACATTCCCAGCAAGCATGCAATTGTATTTTCCTGTCTGTGTTAATATATATGAATTTGTTCTTTATTTGTTTCATAGCTGCATTCTATGATGCTATAGTTTATGGGCATAGGCTCTGTAGCTATTGGGGCCAAAAGCTTAAAACACTTTACTTGATCATTTCTTGTGATGTAAGATATTATCTCCAGCAGGATAAGA
AACGCCACAATGTGCTGGCACTGTCTTGCTGTAGAGTTGTCCTAAACTTTCTGGTAGAATTTTTTTGGCATGAGAAGTTCCTGAATTTTATCTGTAGAAACCAATATTTGATGGGTTCGCTATTTTCTTTCTGATATTTTGTTTCTCGTAAGGTTGAATTTGGAATTAAATTGTCTTATAGTTATATACTTTGATGTGTC
ATTGCCATCATCATCCAGAGATGGATCTAATTATGTATAATAAATATACGATTGAGTTGGAAGTTGGAACATGATACTTTGTTTCCATCATGATTATATGTAACATCTACAGGTTATATATGTCCTGAGATGGGCATTCTAGTATATAATATCTTGTATTTTGCATTCAATTTGATAGTAAAAAACAGTTTACCAT


>TRINITY_DN43839_c1_g3_i9 from sample A9 / Example unspliced isoform:
TGCGCACTAAGTCAGTGTGACTACCTCGCCAGCAATTTTGGTCTCCGATTCTGCATGATATACCGTCATATCCACCTCCAGCTCTATTTCCATTTCCAATACCGATTTCCTCCCTTCGCTCTTCTTAAAGGGAAAGTAAAGTATGGTAAATGTTTGCACTCAATTCTCCCTCCGTCCCATTAATTATTGTATTGTTTTTC
ACCAATTTAGATTAGATTAGAACCCCCAAGTGCCGAACCTCAAGACCAAAGGAACAAAGGGAAGGCATTGATTAATTATTAACCAAAGCATTGGGTCGTTTCAGCCAGGGAATTGAATTTTGGGCACCCATGGTTGTTACTTCCCAGACGGACTAGCTATCATTCTCTGTTTCCTTTTGGCGGGGGATTTTGGATTGGAT
TGGTCTGGATGGGAGCAAATGAAGTTAAGAGAAAAAAAATAATTAAAAACTGTTGTGGGGTTTGAGGTGAAAATTCCGAACAAGTGTATTTGCAGTAGTCGAGGAATCATCAAATACATATATACATATATCCGGATATCATTTCATTTGTTGGAAGTAGCGGAAGAAGAAGAGTAGAAGATAGTTATGGGTGGCAACGG
CAACGAAGAGAGGAGGAGGAGGGACGGATCAGCTGCAGTGTACAAAACGAAGGTGGTGCACTTTCAGGGGCGATCCATCCCTATTATTCTTCAGAACGACAATGGGCCTTGTCCTCTTCTAGCAATAAGCAATGTTCTTCTTTTACGGAACATCATAAATTTGAGCCCAGATACAACAGAGATCTCTTTGCAAAGGTTGC
TTTCACTTGTGGCTGGAAGATTGCTGGACTCGAACAGCAATATTGAGAACAAAGATGATGGGTATGTGCAAAATCAGCAGCAGAACATTGCGGATGCTATTGATTTACTTCCCTGCCTTGCAACTGGGATAGATGTTAATGTTCGCTTCAGACATATACATGACTTTGAGTTTACGCGAGAGTGTGCAATATTTGACCTG
TTAGACATCGGGCTGGTGCATGGCTGGATTTTTGATCCTCAGGATGAAGAGACTGCAAAAGTAATAGGATCACATTCATATAATACCCTCATGGAAAAGCTTGTAGCATTGGATGCGAGCAAATCACAGGAGTTGCCTGCTAGAGTTGCTGAAGAAGATTCGATCGATTTTGCTGCTGCCACCACTGCAACTTTAGGGGT
TCCTTCTCCATCCCTATCAAGGGGAATATCATTTGAAGGCTCGCCCGTTTCAACTCCTAATGACAGCAGGGGAAGGAAGGGTGATGAACAGGAAGTTGAAGAACTTATGAAAGCATTGGATTTATCAAAAGTTGATTCAATAGCTGGTGCTCCACCTGATACTGATACCAGTTTGTCTGCTATTTCACATCAAAATCTGA
ATTCTGAGAGACCAGCATGTCAAAGTTTGTTTGAATCATCCGAGCATAACATTGAAGATGCAGATCATGCAAGTCATATGGATGAAGCCAGTAAATTGGATGGTGATATAGCTGTTAGCGGTAAGAGCAGGGTTGCTTCTTTCAGTTGTCTGAATGAATTAAATATATCCTCTGCTATTGTGCTTGATAAAACTCTTATT
GAAGAGCTATTGATTGGGAGAACAGGAGATGATGAAAATTCTCTAAAATATAAAGgtgaggtagcagctgaaaatgcagttttggatgcacaatttttgttcccacaaagttcaaatgtctctcctgaacttcatactgatctatgtactgcagAAGAGACTTCCAAAAGTCAGCAAGTAGTGGGAAGGAGTTCTTGTCA
AGAAACGTCAACAAGTGTAGTTCATTGTAGGCCAGTAGAAGGTCTGGGGGAACATGACTATGCTGTTTATACCAATGCCATTTCATGTGGGGGTGTTCCACTCTCTGACAATAAGGAAGAACCAATTGACAGCTCAGAAGATGTGAGCTCAAGTTTAGAGGGTAGTGAGCCTATTTATGAAGGGGAGGAATGCATTCTAG
TGGATCCTGCACCTCAGATGTATGAAAGTCGAGAGCCTTTATATGAAGGTGAAATGGTTTTAGCAGAGCAGGCTGACAAAGGAGTAATTTTACAAAGTATAGGGTTGGGATCTGGAGATGAAATTTCAATTCAAGAACAGGAAGAATCTCTGAGTGCAGCAGATAGAGAAGGAACACTGATTCGGAATTTTTTGAGGAGT
AATGCCAGTCAATTGACAGTTTTTGGGCTTTTTTGTCTGCAAGAAGGATTGAAGGACTGCGAACTCTGTGTTTTTTTCCGTAACAATCACTTTAGTACAATGTTTAAGTTCAAGGGGGATCTCTACCTCCTAGCAACAGATCAAGGTTATATAAATCAGCCTGATTTGGTGTGGGAAAAACTTAATGAGGTCCATGGAGA
TTCTGTGTTCATGACTGGGGACTTCAAGGAGTTCAAAGCAGAAGATCAAGTTGGCAGCTCATGGAATCAACATCATGCTGTGGCTAAAACTGCAGATTATATTGCCTCTGTGACCAATACGCAGCCGTCAGATGCAAATTTCAATTCAGATTTGCAACTTGCAATTGCTCTTCAGCAACAAGAATTTGAGCAGCAGCAGC
AGCAGCAGCAACAACAACAACAACAACAACATTTGCAACACTATCCGCGTGCTACTCAGTCATCAGTGCCTGCTAATTCCAAGATGATTACAGGTCCAGAGGCACCCAAGGCCAGCTCCTCTACGTCTAAGCAGGAAGCAAGGAAGGAGAGGTGTGTTGTAATGTGACATTCCCAGCAAGCATGCAATTGTATTTTCCTG
TCTGTGTTAATATATATGAATTTGTTCTTTATTTGTTTCATAGCTGCATTCTATGATGCTATAGTTTATGGGCATAGGCTCTGTAGCTATTGGGGCCAAAAGCTTAAAACACTTTACTTGATCATTTCTTGTGATGTAAGATATTATCTCCAGCAGGATAAGAAACGCCACAATGTGCTGGCACTGTCTTGCTGTAGAGT
TGTCCTAAACTTTCTGGTAGAATTTTTTTGGCATGAGAAGTTCCTGAATTTTATCTGTAGAAACCAATATTTGATGGGTTCGCTATTTTCTTTCTGATATTTTGTTTCTCGTAAGGTTGAATTTGGAATTAAATTGTCTTATAGTTATATACTTTGATGTGTCATTGCCATCATCATCCAGAGATGGATCTAATTATGTA
TAATAAATATACGATTGAGTTGGAAGTTGGAACATGATACTTTGTTTCCATCATGATTATATGTAACATCTACAGGTTATATATGTCCTGAGATGGGCATTCTAGTATATAATATCTTGTATTTTGCATTCAATTTGATAGTAAAAAACAGTTTACCAT



Intron 4 GTAAGAGCAG ... AAATATAAAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A146.3411127
A265.3891420
A356.67132212
A43616153
A5201434
A655.56464
A750.77162112
A856.19149
A941.18151011
A1050141117



>TRINITY_DN43839_c1_g3_i11 from sample A9 / Example spliced isoform
TTCTACTCTTCTTCTTCCGCTACTTCCATCAAATACATATATACATATATCCGGATATCATTTCATTTGTTGGAAGTAGCGGAAGAAGAAGAGTAGAAGATAGTTATGGGTGGCAACGGCAACGAAGAGAGGAGGAGGAGGGACGGATCAGCTGCAGTGTACAAAACGAAGGTGGTGCACTTTCAGGGGCGATCCATCCC
TATTATTCTTCAGAACGACAATGGGCCTTGTCCTCTTCTAGCAATAAGCAATGTTCTTCTTTTACGGAACATCATAAATTTGAGCCCAGATACAACAGAGATCTCTTTGCAAAGGTTGCTTTCACTTGTGGCTGGAAGATTGCTGGACTCGAACAGCAATATTGAGAACAAAGATGATGGGTATGTGCAAAATCAGCAGC
AGAACATTGCGGATGCTATTGATTTACTTCCCTGCCTTGCAACTGGGATAGATGTTAATGTTCGCTTCAGACATATACATGACTTTGAGTTTACGCGAGAGTGTGCAATATTTGACCTGTTAGACATCGGGCTGGTGCATGGCTGGATTTTTGATCCTCAGGATGAAGAGACTGCAAAAGTAATAGGATCACATTCATAT
AATACCCTCATGGAAAAGCTTGTAGCATTGGATGCGAGCAAATCACAGGAGTTGCCTGCTAGAGTTGCTGAAGAAGATTCGATCGATTTTGCTGCTGCCACCACTGCAACTTTAGGGGTTCCTTCTCCATCCCTATCAAGGGGAATATCATTTGAAGGCTCGCCCGTTTCAACTCCTAATGACAGCAGGGGAAGGAAGGG
TGATGAACAGGAAGTTGAAGAACTTATGAAAGCATTGGATTTATCAAAAGTTGATTCAATAGCTGGTGCTCCACCTGATACTGATACCAGTTTGTCTGCTATTTCACATCAAAATCTGAATTCTGAGAGACCAGCATGTCAAAGTTTGTTTGAATCATCCGAGCATAACATTGAAGATGCAGATCATGCAAGTCATATGG
ATGAAGCCAGTAAATTGGATGGTGATATAGCTGTTAGCGAAGAGACTTCCAAAAGTCAGCAAGTAGTGGGAAGGAGTTCTTGTCAAGAAACGTCAACAAGTGTAGTTCATTGTAGGCCAGTAGAAGGTCTGGGGGAACATGACTATGCTGTTTATACCAATGCCATTTCATGTGGGGGTGTTCCACTCTCTGACAATAAG
GAAGAACCAATTGACAGCTCAGAAGATGTGAGCTCAAGTTTAGAGGGTAGTGAGCCTATTTATGAAGGGGAGGAATGCATTCTAGTGGATCCTGCACCTCAGATGTATGAAAGTCGAGAGCCTTTATATGAAGGTGAAATGGTTTTAGCAGAGCAGGCTGACAAAGGAGTAATTTTACAAAGTATAGGGTTGGGATCTGG
AGATGAAATTTCAATTCAAGAACAGGAAGAATCTCTGAGTGCAGCAGATAGAGAAGGAACACTGATTCGGAATTTTTTGAGGAGTAATGCCAGTCAATTGACAGTTTTTGGGCTTTTTTGTCTGCAAGAAGGATTGAAGGACTGCGAACTCTGTGTTTTTTTCCGTAACAATCACTTTAGTACAATGTTTAAGTTCAAGG
GGGATCTCTACCTCCTAGCAACAGATCAAGGTTATATAAATCAGCCTGATTTGGTGTGGGAAAAACTTAATGAGGTCCATGGAGATTCTGTGTTCATGACTGGGGACTTCAAGGAGTTCAAAGCAGAAGATCAAGTTGGCAGCTCATGGAATCAACATCATGCTGTGGCTAAAACTGCAGATTATATTGCCTCTGTGACC
AATACGCAGCCGTCAGATGCAAATTTCAATTCAGATTTGCAACTTGCAATTGCTCTTCAGCAACAAGAATTTGAGCAGCAGCAGCAGCAGCAGCAACAACAACAACAACAACAACATTTGCAACACTATCCGCGTGCTACTCAGTCATCAGTGCCTGCTAATTCCAAGATGATTACAGGTCCAGAGGCACCCAAGGCCAG
CTCCTCTACGTCTAAGCAGGAAGCAAGGAAGGAGAGGTGTGTTGTAATGTGACATTCCCAGCAAGCATGCAATTGTATTTTCCTGTCTGTGTTAATATATATGAATTTGTTCTTTATTTGTTTCATAGCTGCATTCTATGATGCTATAGTTTATGGGCATAGGCTCTGTAGCTATTGGGGCCAAAAGCTTAAAACACTTT
ACTTGATCATTTCTTGTGATGTAAGATATTATCTCCAGCAGGATAAGAAACGCCACAATGTGCTGGCACTGTCTTGCTGTAGAGTTGTCCTAAACTTTCTGGTAGAATTTTTTTGGCATGAGAAGTTCCTGAATTTTATCTGTAGAAACCAATATTTGATGGGTTCGCTATTTTCTTTCTGATATTTTGTTTCTCGTAAG
GTTGAATTTGGAATTAAATTGTCTTATAGTTATATACTTTGATGTGTCATTGCCATCATCATCCAGAGATGGATCTAATTATGTATAATAAATATACGATTGAGTTGGAAGTTGGAACATGATACTTTGTTTCCATCATGATTATATGTAACATCTACAGGTTATATATGTCCTGAGATGGGCATTCTAGTATATAATAT
CTTGTATTTTGCATTCAATTTGATAGTAAAAAACAGTTTACCAT


>TRINITY_DN43839_c1_g3_i13 from sample A9 / Example unspliced isoform:
AGGTGAGGTAGCAGCTGATGAAGCCAGTAAATTGGATGGTGATATAGCTGTTAGCGgtaagagcagggttgcttctttcagttgtctgaatgaattaaatatatcctctgctattgtgcttgataaaactcttattgaagagctattgattgggagaacaggagatgatgaaaattctctaaaatataaagAAGAGACTT
CCAAAAGTCAGCAAGTAGTGGGAAGGAGTTCTTGTCAAGAAACGTCAACAAGTGTAGTTCATTGTAGGCCAGTAGAAGGTCTGGGGGAACATGACTATGCTGTTTATACCAATGCCATTTCATGTGGGGGTGTTCCACTCTCTGACAATAAGGAAGAACCAATTGACAGCTCAGAAGATGTGAGCTCAAGTTTAGAGGGT
AGTGAGCCTATTTATGAAGGGGAGGAATGCATTCTAGTGGATCCTGCACCTCAGATGTATGAAAGTCGAGAGCCTTTATATGAAGGTGAAATGGTTTTAGCAGAGCAGGCTGACAAAGGAGTAATTTTACAAAGTATAGGGTTGGGATCTGGAGATGAAATTTCAATTCAAGAACAGGAAGAATCTCTGAGTGCAGCAGA
TAGAGAAGGAACACTGATTCGGAATTTTTTGAGGAGTAATGCCAGTCAATTGACAGTTTTTGGGCTTTTTTGTCTGCAAGAAGGATTGAAGGACTGCGAACTCTGTGTTTTTTTCCGTAACAATCACTTTAGTACAATGTTTAAGTTCAAGGGGGATCTCTACCTCCTAGCAACAGATCAAGGTTATATAAATCAGCCTG
ATTTGGTGTGGGAAAAACTTAATGAGGTCCATGGAGATTCTGTGTTCATGACTGGGGACTTCAAGGAGTTCAAAGCAGAAGATCAAGTTGGCAGCTCATGGAATCAACATCATGCTGTGGCTAAAACTGCAGATTATATTGCCTCTGTGACCAATACGCAGCCGTCAGATGCAAATTTCAATTCAGATTTGCAACTTGCA
ATTGCTCTTCAGCAACAAGAATTTGAGCAGCAGCAGCAGCAGCAGCAACAACAACAACAACAACAACATTTGCAACACTATCCGCGTGCTACTCAGTCATCAGTGCCTGCTAATTCCAAGATGATTACAGGTCCAGAGGCACCCAAGGCCAGCTCCTCTACGTCTAAGCAGGAAGCAAGGAAGGAGAGGTGTGTTGTAAT
GTGACATTCCCAGCAAGCATGCAATTGTATTTTCCTGTCTGTGTTAATATATATGAATTTGTTCTTTATTTGTTTCATAGCTGCATTCTATGATGCTATAGTTTATGGGCATAGGCTCTGTAGCTATTGGGGCCAAAAGCTTAAAACACTTTACTTGATCATTTCTTGTGATGTAAGATATTATCTCCAGCAGGATAAGA
AACGCCACAATGTGCTGGCACTGTCTTGCTGTAGAGTTGTCCTAAACTTTCTGGTAGAATTTTTTTGGCATGAGAAGTTCCTGAATTTTATCTGTAGAAACCAATATTTGATGGGTTCGCTATTTTCTTTCTGATATTTTGTTTCTCGTAAGGTTGAATTTGGAATTAAATTGTCTTATAGTTATATACTTTGATGTGTC
ATTGCCATCATCATCCAGAGATGGATCTAATTATGTATAATAAATATACGATTGAGTTGGAAGTTGGAACATGATACTTTGTTTCCATCATGATTATATGTAACATCTACAGGTTATATATGTCCTGAGATGGGCATTCTAGTATATAATATCTTGTATTTTGCATTCAATTTGATAGTAAAAAACAGTTTACCAT



Intron 5 GTAGGTATAT ... CTTGTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A15.568551
A227.529110
A317.5447107
A44.554220
A5252481
A6-1000
A715.791630
A80400
A90400
A1042410



>TRINITY_DN121844_c5_g5_i3 from sample A3 / Example spliced isoform
CGGATTGATTAGCGATAACATAAAATATTAATTAGCGAAAGATCGCGAGGAAGGGAAGGGTTCCAATTTCACATCTGTTGCTATCTCCATTTCCCTTCCCTCGACTTGATAAGTAGTAAGCGCATCACCTCCCTTGGTCGATTACTTTTAATTTCAGCGACATACAGCCCGATCTCTCTGCGCGCACGGACTTAAGACCC
CAGCCTTGGTATATCCCGCACTTGTTTCGCATTTGCCCTTGCTGCTGCCACTGCTTCAACCGCAAATCCCTGTCGTATCCTCGCCCTCCTTTACGAGCCATGGGTTTCATCCTTCCGAAACCCCATATGGTTTGAATTTGCATACCCCGTCCTGTTCTTTAGTAATTTCTTCCCGTACATTCGATTTTCAGGGCACAATC
TGCAAGAGTAGCGAACCTAACGAGGTAGTCGTTAAATACCCCTTGGATTTGGTTTTTGTTCGAGGCGTAAGAATGGCCGAAAGGAAGTCCGATCTTCCGCAGCATTCTGAGACGCAGGATCATCCGAATATCAAAGTGTTGTGCTTAGATGGAGACAGCTCGGGATTGTTGTCTGGTAACCCTGATGCGGAATTTAAAGA
TGTATCTGGGCCTGAAAATGTAATCCCTCTTTCTCCTCAATGGCTGCACTCAAGGGTTGGTGAGACCAAGGAACCGCGGACTCCAAACAGTTTTAGTCAGAGTGGAAGCACTGATGCAAATCTCAAAGAGCAATGGAGAGGAGAGGGTTCTCTTGACAAGAAAGATTGGCGGAGAACTGCTCCTGGTGAGAGCGACGGTG
GTCGGCGCTGGCGTGAAGAGGAAAGGGAAAATACTGCAGTTGCCAGAAGAGACCGTAGAAAAGAAGGGGACAGAGAAGTTGAAACTCGGAAGGTTGATCGTAGATCAGAGAATCCTTCACTAAGAGAAGTTGCAGACAATAAGCATCCAAATTCGTCAGACCGTTGGCAAGATACTACTGCCCGTGATCCAGGCCACGAG
GCACGTCGTGACAGGAAATGGTCCACAGCATGGGGACCTGAAAACAATGAAAAAGATATACGTCGAGAGAAACGCAGTGAAGTGGACAAGGAGGATGGTGGTCTTGATAGACAACCTTCTTTTTCAAGCAGTCGTCTATTGGCTGCAGACCGTGAATCGGAAAAATCATCGACTGACAAGTGGAGACCATCCAGACTCCG
GGACAAACTTAAAACACAAAAGGATGGTGTTGGATTCCTTGCAGGTCGAGGCCGTGGAAATGGTTTGACAAATAGTCTTTCGATGAGAACTCCTATTGGTGCTTCTACTATTACAAATGCTTTTTGCTACCCACGGGCCAAACTTCTTGACATTTATAGGGATTGTGGATCTTTCTCACCTTTACGAGGTGTTGCCGATG
GATTTGAGGAAGCTTCTGGCGTTACTTCTTCTGACCATTTTGAGCCATTGGCATTTGTCTCGCCTGAGCCAGAAGAGCAGGCTGTATTAGAAGACATCTCTAAGGGGAAAATTGCAGGCGGTGAAGCAATCCGCAGGACATTTAACAATACATCTGGCAGGATCAATATTGATGGCCTTGGAATTAGAGAGGAGATATTG
TCCAAGAGCAATAAGAAAAGGGATAGTGATGAATCTGTGAGCTGTGTGATGGATGATTCTCAATTAAAGGTGCATGAAGATGTGCTCCCTGATTTAGATGAGTCTGAACTTGTAGATTCAGTTTCCAAGGAGATCACAGCAGGCAGCAAAGATAAGACAATGTATTCAGTTCCCACATTATATGATTTCAATGATGAGAT
ATTCATCCCAAAGGCTTCTGCTGAGGCAAGGAGGGCTGCCAAATCTTTTGAAGGAATATCTGAATATCCTTTCGTTGAAGAGATGATGGGTGAGGAGGTTTGTCATGAAAATGAAAATGTAAATTCATCAGGACTAGGTAATTCTTATGATAATCTATCCATGAAAGGAGAAGCATCAATGGCAGTTGGGGAGAGGAAGA
GTACAAAAGATATTGAAGTGACTTCTTCTGAGCTGCCTATCGGGCTACCATCTGATGCAAGTGTGCTTTTTGAAGACAAATCCTTTGAAGGGACTCCTGTAGAGTGTGATTTCGAGGATGGAAAATCAGGAAATAAGGATGGCATCAAGCTAGGAGAACAAGGTTTCCTGCTTGAGGAACTAAGTCTTTTTTATAAGGAT
CCTCAAGGGGATATTCAGGGGCCTTTTCTAGGTATAGATATAATTCAATGGTTTGAACAAGGTTTTTTTGGTACAGATTTACTAGTGTGCCTTTCTGATGCTCCGGAGGGAACACCATTTCAGCCCCTTGGAGAGCTTATTCTACACATGAGACATAAAGCTCAATCATCTCATGGTACTGTCTTTGAGGATTCAGAAGA
CACCAAAGGAATGAGTTCAAGAAGTGGTCATGATATTTCATCTAAAAAGGCTGGTATCATATATGAACATAATTCGATACTCAATCCTGAGCAAGAAAAGACATTGTCCGACTTTTCAGAGCAGGTCAATGCAGGATTAACTGGCAGAGGACTATCTTTTCCTGATATGCTCAAAGGTACTAAGAATAATACACTTGATC
ATCAAGAACAACACTTTTTGGAATCTGCTACAGAGAAAAATGCAGAAATTCTTGCAAGAATAATTCATCATGGAATACCACCGGGAGAATCTATTGGAAGTCTTGAGAGGCAGAGAATGTCTTCCTTACCTCGGTTTGATACAGATGATGGCCTTGTTCCTTGGCAATCTGAAGCAAATGCTTTTGGTTCAGTCTGGCCT
CAGCAGGAAGCGGATCAGTTCATTGATATAAGATCCGGTCTGCTGGATGCCACAGTTAGCAGTTCCATTTCTGGTAATATGCTTTCAATGATGTCAAGGATGCAATCCCTACCTAATAATCAGCGACAAGACTTATTTCATCAATCAAATGGTGCCGGCACTTCTCCTCCAATTGATAGGGCTTGGTCTGAAACATTTCA
GAGGGATGCATATTCCACTAACAAAATTTTCAATGTTGCATCTGATCCTCATCGTATGACACATCCTGAACAGAATTTGCAAAGAATAGAGCGAGCTAACCTTTTATTTCATCACCAGTTGGAGCAACAGAAGCAGAAACAGCAACAGCAGCAGCAACAACAACAACAAATGCTGATGCAACGGCAATTGCAGTCCGGAG
CTAAATTGCTGGATCACAGTACAATTTCAGGTATGGGTCATAACTTTGACTCTTCACTGTTGCAACACCACCAATTGCTACAACCACCTTTACAACCACGTCAGCAACTACCTCCATCTGCGCATGTTATGGACCCAATCTCAAGGCTTCAGCTGCAGCATCACCATCACCAACCCCAACACCATCTACAACTACAACCA
CATCAGCAGCAAACCCAGCATAATATGCAGATGGAGCACCAACGGCAGTCTCATCAAACAGGCCTAGATCAGTTACTGTCTCATCAGATATATGATCCTAGCTATGCTCAGAATCAATTTCCTTCTGGTGGAAGTAGCATTGATCAGCTTATGTTAAGGCAAGAAGGTATTAGGCATGAATCCCAGCCACATCATCAGTC
TATACGTAGGCAAAGCAATTCAATGGACCCAAGCCTTTCATTGGAACAACTTTTGCATCAGAGAAGACATGAGCTATCAGCTAACCAGTTTCCCTTGCCATCGCAACGGCAATTTATTGATGAAAGACGTGTAAGTGGAGTTTGGGCTGTTGATGAGTCTGGTGAGTTTGTACAGACGCAAGCACCTACTCTCCATCCCT
TTGATTTATACCAGCCGCATCAGCAGATACTGAATTCACAACCACAACACACACAATTATTAAATTTTCAATCTTCAAGCACCCTAGCATCTAATTCAGAAATAAAATATCCTTACCTATTTGAGCGGGCTGAGCATCATGACCATAGCAGTGAAGGTCGATCATTGCATGGTATGCATGATCCCAACCTGTTACCTTTT
GAAAGGCCTACTGATTTGTCTTCGCTGAACATGGACTTCTCACATTCTCTTAAACAAGCCCAGGAACGCAGGGTGCAGGAAAGGTATAATGTTACACGTTTGGCAGGACAGGATGAACAATTCCCATCAGAAGTTACCTACAATTCCCGATCTCATCCAATGGCCTTCCATGGTATACAATCTGAGGTTACAAATGGGAT
TTGGGAGCAACCCTCCAGCACACATTCTACTGATCTTCATGTTCAGGGTCCTGGCTTTTTTATGCAGCAAGGACAACCTTTCGATTTTATTACTGAAAGCCAAGGTCAGAAGTTGCATCGAGTTGGGTTAAATTGGCAGTCCAATGAAGGTTCTCAAGAGCATGTTTCTTCTGAAGAATTGCACCGGAGATCTTTAATTT
CTACACACACTGCTGATTTAGAGACAAGACAATCTCATGGCGACTCGCTTCATCAAAAATTCCCTCTTCGTTCTGGTTCGTCATCTGTAATGCCTGCTATTCAAAAATCGTAGAAATGAAGATCTTTCAGAGGTTCTAGAAGATTTGGCTGGGAATGGTGTTGGATCGACTTATATGGCCTGTAACTCAGCATCTGAAAT
GCAGATGGGGACTGGCTTATCTGTAGATAGTGTATATGATGGTATGCATTCAGAAGCATTTATTGAGGAGCCATTTTATTCATCAAATAACAATGAGCTGCAACTGTCTCTGCAAGTGTCTGATTCACTCGGGCAATCCTCACGAGATGACATTGAGAGTTTGGAATTCAAAGAAGCTAAAAAAAACAAACGGCGGAGTT
CTAAAGCTAGAGGGACAGTAAAAATGACTGTTGTTTCTTCAGATGCGTCTTCAGTGTCAAACTTATCTACAGGAGCTCCAGAATTGAGTCAAAATCAACAAACAATAAAGCATGACAGTTCCACTGTGCAAGAGTTGTCTTCATGGAGTACAGATTTTAATAGACGAGGGTTATCAGTGCATACTGGCATGGACACTAAA
AAATCCCTACAGAAAACTGGGCAGTTGGTTATTGAAAGCAACAATGCAGATGGGAGAATGAGTCATTCCAGTGACAATGGTGATCAAATATCAACAGTTGAACGGGAAAATTCTAAATTTTCTGCAAAAGCTTTTGAATTGTCAAATTTGGCTTCTTCTCCCTTATCTACAGTACAGAGAAAGCTACTGAGTAACAGAAG
AGCATCTTATGACGCTGGAGAAAGCATTGCAGAAAATGCTGGTGGCAATCTTGCAGTGAAGCCTTCAGAAAGTGCTTTCCAACGGAAAGAAAATTTACCCATTTCAAACTTTAGTAAGACTTCTGCTTCCAGTGATGCTGACACAAGGAATGAAGGAGCAAAATCCTTTCTAAATACATTGAAAAATCCAAAGAAACCAG
AACCAGAAAAAGAGAAGAGCAAAGTTGATTCATCTGCAATTTTAGATTTTGATAGTCAAGGATCAAAAAGTTCAAAAAAGAAGGGCAAGAAAGGAAGGCAGATAGACCCTTCTTTGCTTGGATTTAAGGTTACAAGTAATCGCATCAATATGGGGGAGATTCAACACCCTGATGATTAAATTCCTCTAGTTGATTTGTAT
TCTTAGAGTGGTTATCATAATCCAAGGTAAATATGCCATCATTGATTTGGTTTTTAAATGGGTCATATTCTTAAATTTTTTCTCTGTACAGGTATTTTTGGCATAGTTCCCCCCTGATAGTTTTTTTTCCACTTATTATGAAATTGAATGATTCGAGTCTCTATCAAATGAATTTTCATTTGTTGCACCCAGTAATTGTT
TTCATGTCATTTTACAATGTTTTAGGGAAGACCGGAAATCTACCTTTTGTTTGAGGTGCAATTCTATTCTCTGTTGGTCTGTTCCTCTACTAACAAATTAAAGCCATTTATGCTTGTCACAGTACCTCTTGGTCCTTGCCATTGTGATCATGTTGTTATAGTAGGATTTGATTTATGTAAGTGGTTGAGTCGGTACTATT
TACAAAATATTATAATTAAGTAGAATCTCAACATGTGGCTTTTGTTAATTTTAGTACCTTTTGTTGTCAATTTATGGGACCTGTATATCTATGTTAGATGGAAAAAAGTTTATTGTACAATTTTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTTGATGATCTATGTTAGATGGAAAAATGTTTATCGTACAATT
TTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTCGATGATAACTGTCCAGATTAAATGACATGAGTCACATTATGCAGTAATTTCATGAGCTTTGGCTTTAGATGTTGTGACAGAGGAGTATCTAGTGTCAAAGCTGTCCACCCGGTAATTTTACCTGTCACATCTGCATCTTGCCTAATTTGCATGTCTGGTTAG
TTACCTAAAGGGTTGAGATCATGGGCTAAGTAAAATAATGTGTCCACATGGGCCATACACTAGAAACCTATATGTTGCCCTGGCATACTTAATGGAATAAGTTAGATGCAGCAGTAGTTGCTGCAGGACCTATGACATTGAAACCATAGACTTCCCGAAAGAGGACCAAGTCAACATACATTGATCAATTCTGTATCCAA
AACTCCAGTTACTACATTCTATGTGTTATTTCCTATGTTGCATGGGTACCTGTACCAGGCGGTCGGGTACATGAACCCGTACCGGGTACGCGGGTACTTCAATGGTGCGTTCCCAGCCGTACCCAAATTCAAACTCGCATACCGGGTACTTCACTTATGAAAACAGGGTACTTGCAT


>TRINITY_DN121844_c5_g5_i1 from sample A3 / Example unspliced isoform:
CGGATTGATTAGCGATAACATAAAATATTAATTAGCGAAAGATCGCGAGGAAGGGAAGGGTTCCAATTTCACATCTGTTGCTATCTCCATTTCCCTTCCCTCGACTTGATAAGTAGTAAGCGCATCACCTCCCTTGGTCGATTACTTTTAATTTCAGCGACATACAGCCCGATCTCTCTGCGCGCACGGACTTAAGACCC
CAGCCTTGGTATATCCCGCACTTGTTTCGCATTTGCCCTTGCTGCTGCCACTGCTTCAACCGCAAATCCCTGTCGTATCCTCGCCCTCCTTTACGAGCCATGGGTTTCATCCTTCCGAAACCCCATATGGTTTGAATTTGCATACCCCGTCCTGTTCTTTAGTAATTTCTTCCCGTACATTCGATTTTCAGGGCACAATC
TGCAAGAGTAGCGAACCTAACGAGGTAGTCGTTAAATACCCCTTGGATTTGGTTTTTGTTCGAGGCGTAAGAATGGCCGAAAGGAAGTCCGATCTTCCGCAGCATTCTGAGACGCAGGATCATCCGAATATCAAAGTGTTGTGCTTAGATGGAGACAGCTCGGGATTGTTGTCTGGTAACCCTGATGCGGAATTTAAAGg
taggtatatatggttatatcttttcgctctgtatatccactatacatagtagacacgcttgtttgttgagatggttcatgacacttgttgcagATGTATCTGGGCCTGAAAATGTAATCCCTCTTTCTCCTCAATGGCTGCACTCAAGGGTTGGTGAGACCAAGGTATTTGCAACACTTCTTCAATAGGGCTTTGTAAGA
TTTGATTTATTGTCTCTAGATTTTACTTGATTGAGATAAAGTGATTACCCACTGCAATACTGCCATATTCGACGCTTTGAATTGTTTATTACTATTATTTTTCAATGTCTTAGTTCTAACAACATTTGACAAACATAATGTTCTGCACAACAACTTCAATTGATAGGTATATCTTTTGTTTTAACTATCTGTATGGTTGT
TGCTGCTATGTTTTTATATTTTGCTTTAGGAACCGCGGACTCCAAACAGTTTTAGTCAGAGTGGAAGCACTGATGCAAATCTCAAAGAGCAATGGAGAGGAGAGGGTTCTCTTGACAAGAAAGATTGGCGGAGAACTGCTCCTGGTGAGAGCGACGGTGGTCGGCGCTGGCGTGAAGAGGAAAGGGAAAATACTGCAGTT
GCCAGAAGAGACCGTAGAAAAGAAGGGGACAGAGAAGTTGAAACTCGGAAGGTTGATCGTAGATCAGAGAATCCTTCACTAAGAGAAGTTGCAGACAATAAGCATCCAAATTCGTCAGACCGTTGGCAAGATACTACTGCCCGTGATCCAGGCCACGAGGCACGTCGTGACAGGAAATGGTCCACAGCATGGGGACCTGA
AAACAATGAAAAAGATATACGTCGAGAGAAACGCAGTGAAGTGGACAAGGAGGATGGTGGTCTTGATAGACAACCTTCTTTTTCAAGCAGTCGTCTATTGGCTGCAGACCGTGAATCGGAAAAATCATCGACTGACAAGTGGAGACCATCCAGACTCCGGGACAAACTTAAAACACAAAAGGATGGTGTTGGATTCCTTG
CAGGTCGAGGCCGTGGAAATGGTTTGACAAATAGTCTTTCGATGAGAACTCCTATTGGTGCTTCTACTATTACAAATGCTTTTTGCTACCCACGGGCCAAACTTCTTGACATTTATAGGGATTGTGGATCTTTCTCACCTTTACGAGGTGTTGCCGATGGATTTGAGGAAGCTTCTGGCGTTACTTCTTCTGACCATTTT
GAGCCATTGGCATTTGTCTCGCCTGAGCCAGAAGAGCAGGCTGTATTAGAAGACATCTCTAAGGGGAAAATTGCAGGCGGTGAAGCAATCCGCAGGACATTTAACAATACATCTGGCAGGATCAATATTGATGGCCTTGGAATTAGAGAGGAGATATTGTCCAAGAGCAATAAGAAAAGGGATAGTGATGAATCTGTGAG
CTGTGTGATGGATGATTCTCAATTAAAGGTGCATGAAGATGTGCTCCCTGATTTAGATGAGTCTGAACTTGTAGATTCAGTTTCCAAGGAGATCACAGCAGGCAGCAAAGATAAGACAATGTATTCAGTTCCCACATTATATGATTTCAATGATGAGATATTCATCCCAAAGGCTTCTGCTGAGGCAAGGAGGGCTGCCA
AATCTTTTGAAGGAATATCTGAATATCCTTTCGTTGAAGAGATGATGGGTGAGGAGGTTTGTCATGAAAATGAAAATGTAAATTCATCAGGACTAGGTAATTCTTATGATAATCTATCCATGAAAGGAGAAGCATCAATGGCAGTTGGGGAGAGGAAGAGTACAAAAGATATTGAAGTGACTTCTTCTGAGCTGCCTATC
GGGCTACCATCTGATGCAAGTGTGCTTTTTGAAGACAAATCCTTTGAAGGGACTCCTGTAGAGTGTGATTTCGAGGATGGAAAATCAGGAAATAAGGATGGCATCAAGCTAGGAGAACAAGGTTTCCTGCTTGAGGAACTAAGTCTTTTTTATAAGGATCCTCAAGGGGATATTCAGGGGCCTTTTCTAGGTATAGATAT
AATTCAATGGTTTGAACAAGGTTTTTTTGGTACAGATTTACTAGTGTGCCTTTCTGATGCTCCGGAGGGAACACCATTTCAGCCCCTTGGAGAGCTTATTCTACACATGAGACATAAAGCTCAATCATCTCATGGTACTGTCTTTGAGGATTCAGAAGACACCAAAGGAATGAGTTCAAGAAGTGGTCATGATATTTCAT
CTAAAAAGGCTGGTATCATATATGAACATAATTCGATACTCAATCCTGAGCAAGAAAAGACATTGTCCGACTTTTCAGAGCAGGTCAATGCAGGATTAACTGGCAGAGGACTATCTTTTCCTGATATGCTCAAAGGTACTAAGAATAATACACTTGATCATCAAGAACAACACTTTTTGGAATCTGCTACAGAGAAAAAT
GCAGAAATTCTTGCAAGAATAATTCATCATGGAATACCACCGGGAGAATCTATTGGAAGTCTTGAGAGGCAGAGAATGTCTTCCTTACCTCGGTTTGATACAGATGATGGCCTTGTTCCTTGGCAATCTGAAGCAAATGCTTTTGGTTCAGTCTGGCCTCAGCAGGAAGCGGATCAGTTCATTGATATAAGATCCGGTCT
GCTGGATGCCACAGTTAGCAGTTCCATTTCTGGTAATATGCTTTCAATGATGTCAAGGATGCAATCCCTACCTAATAATCAGCGACAAGACTTATTTCATCAATCAAATGGTGCCGGCACTTCTCCTCCAATTGATAGGGCTTGGTCTGAAACATTTCAGAGGGATGCATATTCCACTAACAAAATTTTCAATGTTGCAT
CTGATCCTCATCGTATGACACATCCTGAACAGAATTTGCAAAGAATAGAGCGAGCTAACCTTTTATTTCATCACCAGTTGGAGCAACAGAAGCAGAAACAGCAACAGCAGCAGCAACAACAACAACAAATGCTGATGCAACGGCAATTGCAGTCCGGAGCTAAATTGCTGGATCACAGTACAATTTCAGGTATGGGTCAT
AACTTTGACTCTTCACTGTTGCAACACCACCAATTGCTACAACCACCTTTACAACCACGTCAGCAACTACCTCCATCTGCGCATGTTATGGACCCAATCTCAAGGCTTCAGCTGCAGCATCACCATCACCAACCCCAACACCATCTACAACTACAACCACATCAGCAGCAAACCCAGCATAATATGCAGATGGAGCACCA
ACGGCAGTCTCATCAAACAGGCCTAGATCAGTTACTGTCTCATCAGATATATGATCCTAGCTATGCTCAGAATCAATTTCCTTCTGGTGGAAGTAGCATTGATCAGCTTATGTTAAGGCAAGAAGGTATTAGGCATGAATCCCAGCCACATCATCAGTCTATACGTAGGCAAAGCAATTCAATGGACCCAAGCCTTTCAT
TGGAACAACTTTTGCATCAGAGAAGACATGAGCTATCAGCTAACCAGTTTCCCTTGCCATCGCAACGGCAATTTATTGATGAAAGACGTGTAAGTGGAGTTTGGGCTGTTGATGAGTCTGGTGAGTTTGTACAGACGCAAGCACCTACTCTCCATCCCTTTGATTTATACCAGCCGCATCAGCAGATACTGAATTCACAA
CCACAACACACACAATTATTAAATTTTCAATCTTCAAGCACCCTAGCATCTAATTCAGAAATAAAATATCCTTACCTATTTGAGCGGGCTGAGCATCATGACCATAGCAGTGAAGGTCGATCATTGCATGGTATGCATGATCCCAACCTGTTACCTTTTGAAAGGCCTACTGATTTGTCTTCGCTGAACATGGACTTCTC
ACATTCTCTTAAACAAGCCCAGGAACGCAGGGTGCAGGAAAGGTATAATGTTACACGTTTGGCAGGACAGGATGAACAATTCCCATCAGAAGTTACCTACAATTCCCGATCTCATCCAATGGCCTTCCATGGTATACAATCTGAGGTTACAAATGGGATTTGGGAGCAACCCTCCAGCACACATTCTACTGATCTTCATG
TTCAGGGTCCTGGCTTTTTTATGCAGCAAGGACAACCTTTCGATTTTATTACTGAAAGCCAAGGTCAGAAGTTGCATCGAGTTGGGTTAAATTGGCAGTCCAATGAAGGTTCTCAAGAGCATGTTTCTTCTGAAGAATTGCACCGGAGATCTTTAATTTCTACACACACTGCTGATTTAGAGACAAGACAATCTCATGGC
GACTCGCTTCATCAAAAATTCCCTCTTCGTTCTGGTTCGTCATCTGTAATGCCTGGTAATGTGCTTGCTACTTCTTCTGAACAAAGCCAGTGTATGTGGACTGCTGTGAACTCAAGAGAGTGTATTTCTGGTTGTACTTATGATTCTTCTGATTTGATTTCAAGCCAGCTATTCAAAAATCGTAGAAATGAAGATCTTTC
AGAGGTTCTAGAAGATTTGGCTGGGAATGGTGTTGGATCGACTTATATGGCCTGTAACTCAGCATCTGAAATGCAGATGGGGACTGGCTTATCTGTAGATAGTGTATATGATGGTATGCATTCAGAAGCATTTATTGAGGAGCCATTTTATTCATCAAATAACAATGAGCTGCAACTGTCTCTGCAAGTGTCTGATTCAC
TCGGGCAATCCTCACGAGATGACATTGAGAGTTTGGAATTCAAAGAAGCTAAAAAAAACAAACGGCGGAGTTCTAAAGCTAGAGGGACAGTAAAAATGACTGTTGTTTCTTCAGATGCGTCTTCAGTGTCAAACTTATCTACAGGAGCTCCAGAATTGAGTCAAAATCAACAAACAATAAAGCATGACAGTTCCACTGTG
CAAGAGTTGTCTTCATGGAGTACAGATTTTAATAGACGAGGGTTATCAGTGCATACTGGCATGGACACTAAAAAATCCCTACAGAAAACTGGGCAGTTGGTTATTGAAAGCAACAATGCAGATGGGAGAATGAGTCATTCCAGTGACAATGGTGATCAAATATCAACAGTTGAACGGGAAAATTCTAAATTTTCTGCAAA
AGCTTTTGAATTGTCAAATTTGGCTTCTTCTCCCTTATCTACAGTACAGAGAAAGCTACTGAGTAACAGAAGAGCATCTTATGACGCTGGAGAAAGCATTGCAGAAAATGCTGGTGGCAATCTTGCAGTGAAGCCTTCAGAAAGTGCTTTCCAACGGAAAGAAAATTTACCCATTTCAAACTTTAGTAAGACTTCTGCTT
CCAGTGATGCTGACACAAGGAATGAAGGAGCAAAATCCTTTCTAAATACATTGAAAAATCCAAAGAAACCAGAACCAGAAAAAGAGAAGAGCAAAGTTGATTCATCTGCAATTTTAGATTTTGATAGTCAAGGATCAAAAAGTTCAAAAAAGAAGGGCAAGAAAGGAAGGCAGATAGACCCTTCTTTGCTTGGATTTAAG
GTTACAAGTAATCGCATCAATATGGGGGAGATTCAACACCCTGATGATTAAATTCCTCTAGTTGATTTGTATTCTTAGAGTGGTTATCATAATCCAAGGTAAATATGCCATCATTGATTTGGTTTTTAAATGGGTCATATTCTTAAATTTTTTCTCTGTACAGGTATTTTTGGCATAGTTCCCCCCTGATAGTTTTTTTT
CCACTTATTATGAAATTGAATGATTCGAGTCTCTATCAAATGAATTTTCATTTGTTGCACCCAGTAATTGTTTTCATGTCATTTTACAATGTTTTAGGGAAGACCGGAAATCTACCTTTTGTTTGAGGTGCAATTCTATTCTCTGTTGGTCTGTTCCTCTACTAACAAATTAAAGCCATTTATGCTTGTCACAGTACCTC
TTGGTCCTTGCCATTGTGATCATGTTGTTATAGTAGGATTTGATTTATGTAAGTGGTTGAGTCGGTACTATTTACAAAATATTATAATTAAGTAGAATCTCAACATGTGGCTTTTGTTAATTTTAGTACCTTTTGTTGTCAATTTATGGGACCTGTATATCTATGTTAGATGGAAAAAAGTTTATTGTACAATTTTTTTC
TAGAGGCTGTGTTAGTGGTCAGTAAACTGGTTGATGATCTATGTTAGATGGAAAAATGTTTATCGTACAATTTTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTCGATGATAACTGTCCAGATTAAATGACATGAGTCACATTATGCAGTAATTTCATGAGCTTTGGCTTTAGATGTTGTGACAGAGGAGTATCT
AGTGTCAAAGCTGTCCACCCGGTAATTTTACCTGTCACATCTGCATCTTGCCTAATTTGCATGTCTGGTTAGTTACCTAAAGGGTTGAGATCATGGGCTAAGTAAAATAATGTGTCCACATGGGCCATACACTAGAAACCTATATGTTGCCCTGGCATACTTAATGGAATAAGTTAGATGCAGCAGTAGTTGCTGCAGGA
CCTATGACATTGAAACCATAGACTTCCCGAAAGAGGACCAAGTCAACATACATTGATCAATTCTGTATCCAAAACTCCAGTTACTACATTCTATGTGTTATTTCCTATGTTGCATGGGTACCTGTACCAGGCGGTCGGGTACATGAACCCGTACCGGGTACGCGGGTACTTCAATGGTGCGTTCCCAGCCGTACCCAAAT
TCAAACTCGCATACCGGGTACTTCACTTATGAAAACAGGGTACTTGCAT



Intron 6 GTAAGAGCAG ... TGTACTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A169.44112129
A271.4391926
A372.92133634
A461.45162724
A559.42141328
A669.234810
A764.04163027
A86491616
A962.96151635
A1066.27141837



>TRINITY_DN43839_c1_g3_i11 from sample A9 / Example spliced isoform
TTCTACTCTTCTTCTTCCGCTACTTCCATCAAATACATATATACATATATCCGGATATCATTTCATTTGTTGGAAGTAGCGGAAGAAGAAGAGTAGAAGATAGTTATGGGTGGCAACGGCAACGAAGAGAGGAGGAGGAGGGACGGATCAGCTGCAGTGTACAAAACGAAGGTGGTGCACTTTCAGGGGCGATCCATCCC
TATTATTCTTCAGAACGACAATGGGCCTTGTCCTCTTCTAGCAATAAGCAATGTTCTTCTTTTACGGAACATCATAAATTTGAGCCCAGATACAACAGAGATCTCTTTGCAAAGGTTGCTTTCACTTGTGGCTGGAAGATTGCTGGACTCGAACAGCAATATTGAGAACAAAGATGATGGGTATGTGCAAAATCAGCAGC
AGAACATTGCGGATGCTATTGATTTACTTCCCTGCCTTGCAACTGGGATAGATGTTAATGTTCGCTTCAGACATATACATGACTTTGAGTTTACGCGAGAGTGTGCAATATTTGACCTGTTAGACATCGGGCTGGTGCATGGCTGGATTTTTGATCCTCAGGATGAAGAGACTGCAAAAGTAATAGGATCACATTCATAT
AATACCCTCATGGAAAAGCTTGTAGCATTGGATGCGAGCAAATCACAGGAGTTGCCTGCTAGAGTTGCTGAAGAAGATTCGATCGATTTTGCTGCTGCCACCACTGCAACTTTAGGGGTTCCTTCTCCATCCCTATCAAGGGGAATATCATTTGAAGGCTCGCCCGTTTCAACTCCTAATGACAGCAGGGGAAGGAAGGG
TGATGAACAGGAAGTTGAAGAACTTATGAAAGCATTGGATTTATCAAAAGTTGATTCAATAGCTGGTGCTCCACCTGATACTGATACCAGTTTGTCTGCTATTTCACATCAAAATCTGAATTCTGAGAGACCAGCATGTCAAAGTTTGTTTGAATCATCCGAGCATAACATTGAAGATGCAGATCATGCAAGTCATATGG
ATGAAGCCAGTAAATTGGATGGTGATATAGCTGTTAGCGAAGAGACTTCCAAAAGTCAGCAAGTAGTGGGAAGGAGTTCTTGTCAAGAAACGTCAACAAGTGTAGTTCATTGTAGGCCAGTAGAAGGTCTGGGGGAACATGACTATGCTGTTTATACCAATGCCATTTCATGTGGGGGTGTTCCACTCTCTGACAATAAG
GAAGAACCAATTGACAGCTCAGAAGATGTGAGCTCAAGTTTAGAGGGTAGTGAGCCTATTTATGAAGGGGAGGAATGCATTCTAGTGGATCCTGCACCTCAGATGTATGAAAGTCGAGAGCCTTTATATGAAGGTGAAATGGTTTTAGCAGAGCAGGCTGACAAAGGAGTAATTTTACAAAGTATAGGGTTGGGATCTGG
AGATGAAATTTCAATTCAAGAACAGGAAGAATCTCTGAGTGCAGCAGATAGAGAAGGAACACTGATTCGGAATTTTTTGAGGAGTAATGCCAGTCAATTGACAGTTTTTGGGCTTTTTTGTCTGCAAGAAGGATTGAAGGACTGCGAACTCTGTGTTTTTTTCCGTAACAATCACTTTAGTACAATGTTTAAGTTCAAGG
GGGATCTCTACCTCCTAGCAACAGATCAAGGTTATATAAATCAGCCTGATTTGGTGTGGGAAAAACTTAATGAGGTCCATGGAGATTCTGTGTTCATGACTGGGGACTTCAAGGAGTTCAAAGCAGAAGATCAAGTTGGCAGCTCATGGAATCAACATCATGCTGTGGCTAAAACTGCAGATTATATTGCCTCTGTGACC
AATACGCAGCCGTCAGATGCAAATTTCAATTCAGATTTGCAACTTGCAATTGCTCTTCAGCAACAAGAATTTGAGCAGCAGCAGCAGCAGCAGCAACAACAACAACAACAACAACATTTGCAACACTATCCGCGTGCTACTCAGTCATCAGTGCCTGCTAATTCCAAGATGATTACAGGTCCAGAGGCACCCAAGGCCAG
CTCCTCTACGTCTAAGCAGGAAGCAAGGAAGGAGAGGTGTGTTGTAATGTGACATTCCCAGCAAGCATGCAATTGTATTTTCCTGTCTGTGTTAATATATATGAATTTGTTCTTTATTTGTTTCATAGCTGCATTCTATGATGCTATAGTTTATGGGCATAGGCTCTGTAGCTATTGGGGCCAAAAGCTTAAAACACTTT
ACTTGATCATTTCTTGTGATGTAAGATATTATCTCCAGCAGGATAAGAAACGCCACAATGTGCTGGCACTGTCTTGCTGTAGAGTTGTCCTAAACTTTCTGGTAGAATTTTTTTGGCATGAGAAGTTCCTGAATTTTATCTGTAGAAACCAATATTTGATGGGTTCGCTATTTTCTTTCTGATATTTTGTTTCTCGTAAG
GTTGAATTTGGAATTAAATTGTCTTATAGTTATATACTTTGATGTGTCATTGCCATCATCATCCAGAGATGGATCTAATTATGTATAATAAATATACGATTGAGTTGGAAGTTGGAACATGATACTTTGTTTCCATCATGATTATATGTAACATCTACAGGTTATATATGTCCTGAGATGGGCATTCTAGTATATAATAT
CTTGTATTTTGCATTCAATTTGATAGTAAAAAACAGTTTACCAT


>TRINITY_DN43839_c1_g3_i9 from sample A9 / Example unspliced isoform:
TGCGCACTAAGTCAGTGTGACTACCTCGCCAGCAATTTTGGTCTCCGATTCTGCATGATATACCGTCATATCCACCTCCAGCTCTATTTCCATTTCCAATACCGATTTCCTCCCTTCGCTCTTCTTAAAGGGAAAGTAAAGTATGGTAAATGTTTGCACTCAATTCTCCCTCCGTCCCATTAATTATTGTATTGTTTTTC
ACCAATTTAGATTAGATTAGAACCCCCAAGTGCCGAACCTCAAGACCAAAGGAACAAAGGGAAGGCATTGATTAATTATTAACCAAAGCATTGGGTCGTTTCAGCCAGGGAATTGAATTTTGGGCACCCATGGTTGTTACTTCCCAGACGGACTAGCTATCATTCTCTGTTTCCTTTTGGCGGGGGATTTTGGATTGGAT
TGGTCTGGATGGGAGCAAATGAAGTTAAGAGAAAAAAAATAATTAAAAACTGTTGTGGGGTTTGAGGTGAAAATTCCGAACAAGTGTATTTGCAGTAGTCGAGGAATCATCAAATACATATATACATATATCCGGATATCATTTCATTTGTTGGAAGTAGCGGAAGAAGAAGAGTAGAAGATAGTTATGGGTGGCAACGG
CAACGAAGAGAGGAGGAGGAGGGACGGATCAGCTGCAGTGTACAAAACGAAGGTGGTGCACTTTCAGGGGCGATCCATCCCTATTATTCTTCAGAACGACAATGGGCCTTGTCCTCTTCTAGCAATAAGCAATGTTCTTCTTTTACGGAACATCATAAATTTGAGCCCAGATACAACAGAGATCTCTTTGCAAAGGTTGC
TTTCACTTGTGGCTGGAAGATTGCTGGACTCGAACAGCAATATTGAGAACAAAGATGATGGGTATGTGCAAAATCAGCAGCAGAACATTGCGGATGCTATTGATTTACTTCCCTGCCTTGCAACTGGGATAGATGTTAATGTTCGCTTCAGACATATACATGACTTTGAGTTTACGCGAGAGTGTGCAATATTTGACCTG
TTAGACATCGGGCTGGTGCATGGCTGGATTTTTGATCCTCAGGATGAAGAGACTGCAAAAGTAATAGGATCACATTCATATAATACCCTCATGGAAAAGCTTGTAGCATTGGATGCGAGCAAATCACAGGAGTTGCCTGCTAGAGTTGCTGAAGAAGATTCGATCGATTTTGCTGCTGCCACCACTGCAACTTTAGGGGT
TCCTTCTCCATCCCTATCAAGGGGAATATCATTTGAAGGCTCGCCCGTTTCAACTCCTAATGACAGCAGGGGAAGGAAGGGTGATGAACAGGAAGTTGAAGAACTTATGAAAGCATTGGATTTATCAAAAGTTGATTCAATAGCTGGTGCTCCACCTGATACTGATACCAGTTTGTCTGCTATTTCACATCAAAATCTGA
ATTCTGAGAGACCAGCATGTCAAAGTTTGTTTGAATCATCCGAGCATAACATTGAAGATGCAGATCATGCAAGTCATATGGATGAAGCCAGTAAATTGGATGGTGATATAGCTGTTAGCGgtaagagcagggttgcttctttcagttgtctgaatgaattaaatatatcctctgctattgtgcttgataaaactcttatt
gaagagctattgattgggagaacaggagatgatgaaaattctctaaaatataaaggtgaggtagcagctgaaaatgcagttttggatgcacaatttttgttcccacaaagttcaaatgtctctcctgaacttcatactgatctatgtactgcagAAGAGACTTCCAAAAGTCAGCAAGTAGTGGGAAGGAGTTCTTGTCA
AGAAACGTCAACAAGTGTAGTTCATTGTAGGCCAGTAGAAGGTCTGGGGGAACATGACTATGCTGTTTATACCAATGCCATTTCATGTGGGGGTGTTCCACTCTCTGACAATAAGGAAGAACCAATTGACAGCTCAGAAGATGTGAGCTCAAGTTTAGAGGGTAGTGAGCCTATTTATGAAGGGGAGGAATGCATTCTAG
TGGATCCTGCACCTCAGATGTATGAAAGTCGAGAGCCTTTATATGAAGGTGAAATGGTTTTAGCAGAGCAGGCTGACAAAGGAGTAATTTTACAAAGTATAGGGTTGGGATCTGGAGATGAAATTTCAATTCAAGAACAGGAAGAATCTCTGAGTGCAGCAGATAGAGAAGGAACACTGATTCGGAATTTTTTGAGGAGT
AATGCCAGTCAATTGACAGTTTTTGGGCTTTTTTGTCTGCAAGAAGGATTGAAGGACTGCGAACTCTGTGTTTTTTTCCGTAACAATCACTTTAGTACAATGTTTAAGTTCAAGGGGGATCTCTACCTCCTAGCAACAGATCAAGGTTATATAAATCAGCCTGATTTGGTGTGGGAAAAACTTAATGAGGTCCATGGAGA
TTCTGTGTTCATGACTGGGGACTTCAAGGAGTTCAAAGCAGAAGATCAAGTTGGCAGCTCATGGAATCAACATCATGCTGTGGCTAAAACTGCAGATTATATTGCCTCTGTGACCAATACGCAGCCGTCAGATGCAAATTTCAATTCAGATTTGCAACTTGCAATTGCTCTTCAGCAACAAGAATTTGAGCAGCAGCAGC
AGCAGCAGCAACAACAACAACAACAACAACATTTGCAACACTATCCGCGTGCTACTCAGTCATCAGTGCCTGCTAATTCCAAGATGATTACAGGTCCAGAGGCACCCAAGGCCAGCTCCTCTACGTCTAAGCAGGAAGCAAGGAAGGAGAGGTGTGTTGTAATGTGACATTCCCAGCAAGCATGCAATTGTATTTTCCTG
TCTGTGTTAATATATATGAATTTGTTCTTTATTTGTTTCATAGCTGCATTCTATGATGCTATAGTTTATGGGCATAGGCTCTGTAGCTATTGGGGCCAAAAGCTTAAAACACTTTACTTGATCATTTCTTGTGATGTAAGATATTATCTCCAGCAGGATAAGAAACGCCACAATGTGCTGGCACTGTCTTGCTGTAGAGT
TGTCCTAAACTTTCTGGTAGAATTTTTTTGGCATGAGAAGTTCCTGAATTTTATCTGTAGAAACCAATATTTGATGGGTTCGCTATTTTCTTTCTGATATTTTGTTTCTCGTAAGGTTGAATTTGGAATTAAATTGTCTTATAGTTATATACTTTGATGTGTCATTGCCATCATCATCCAGAGATGGATCTAATTATGTA
TAATAAATATACGATTGAGTTGGAAGTTGGAACATGATACTTTGTTTCCATCATGATTATATGTAACATCTACAGGTTATATATGTCCTGAGATGGGCATTCTAGTATATAATATCTTGTATTTTGCATTCAATTTGATAGTAAAAAACAGTTTACCAT



Intron 7 GTATTTGCAA ... TTTGCTTTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A113.6495115
A225.4244215
A314.065569
A414.755249
A522.731735
A60110
A701500
A80400
A933.33402
A1021.433309



>TRINITY_DN121844_c5_g5_i3 from sample A3 / Example spliced isoform
CGGATTGATTAGCGATAACATAAAATATTAATTAGCGAAAGATCGCGAGGAAGGGAAGGGTTCCAATTTCACATCTGTTGCTATCTCCATTTCCCTTCCCTCGACTTGATAAGTAGTAAGCGCATCACCTCCCTTGGTCGATTACTTTTAATTTCAGCGACATACAGCCCGATCTCTCTGCGCGCACGGACTTAAGACCC
CAGCCTTGGTATATCCCGCACTTGTTTCGCATTTGCCCTTGCTGCTGCCACTGCTTCAACCGCAAATCCCTGTCGTATCCTCGCCCTCCTTTACGAGCCATGGGTTTCATCCTTCCGAAACCCCATATGGTTTGAATTTGCATACCCCGTCCTGTTCTTTAGTAATTTCTTCCCGTACATTCGATTTTCAGGGCACAATC
TGCAAGAGTAGCGAACCTAACGAGGTAGTCGTTAAATACCCCTTGGATTTGGTTTTTGTTCGAGGCGTAAGAATGGCCGAAAGGAAGTCCGATCTTCCGCAGCATTCTGAGACGCAGGATCATCCGAATATCAAAGTGTTGTGCTTAGATGGAGACAGCTCGGGATTGTTGTCTGGTAACCCTGATGCGGAATTTAAAGA
TGTATCTGGGCCTGAAAATGTAATCCCTCTTTCTCCTCAATGGCTGCACTCAAGGGTTGGTGAGACCAAGGAACCGCGGACTCCAAACAGTTTTAGTCAGAGTGGAAGCACTGATGCAAATCTCAAAGAGCAATGGAGAGGAGAGGGTTCTCTTGACAAGAAAGATTGGCGGAGAACTGCTCCTGGTGAGAGCGACGGTG
GTCGGCGCTGGCGTGAAGAGGAAAGGGAAAATACTGCAGTTGCCAGAAGAGACCGTAGAAAAGAAGGGGACAGAGAAGTTGAAACTCGGAAGGTTGATCGTAGATCAGAGAATCCTTCACTAAGAGAAGTTGCAGACAATAAGCATCCAAATTCGTCAGACCGTTGGCAAGATACTACTGCCCGTGATCCAGGCCACGAG
GCACGTCGTGACAGGAAATGGTCCACAGCATGGGGACCTGAAAACAATGAAAAAGATATACGTCGAGAGAAACGCAGTGAAGTGGACAAGGAGGATGGTGGTCTTGATAGACAACCTTCTTTTTCAAGCAGTCGTCTATTGGCTGCAGACCGTGAATCGGAAAAATCATCGACTGACAAGTGGAGACCATCCAGACTCCG
GGACAAACTTAAAACACAAAAGGATGGTGTTGGATTCCTTGCAGGTCGAGGCCGTGGAAATGGTTTGACAAATAGTCTTTCGATGAGAACTCCTATTGGTGCTTCTACTATTACAAATGCTTTTTGCTACCCACGGGCCAAACTTCTTGACATTTATAGGGATTGTGGATCTTTCTCACCTTTACGAGGTGTTGCCGATG
GATTTGAGGAAGCTTCTGGCGTTACTTCTTCTGACCATTTTGAGCCATTGGCATTTGTCTCGCCTGAGCCAGAAGAGCAGGCTGTATTAGAAGACATCTCTAAGGGGAAAATTGCAGGCGGTGAAGCAATCCGCAGGACATTTAACAATACATCTGGCAGGATCAATATTGATGGCCTTGGAATTAGAGAGGAGATATTG
TCCAAGAGCAATAAGAAAAGGGATAGTGATGAATCTGTGAGCTGTGTGATGGATGATTCTCAATTAAAGGTGCATGAAGATGTGCTCCCTGATTTAGATGAGTCTGAACTTGTAGATTCAGTTTCCAAGGAGATCACAGCAGGCAGCAAAGATAAGACAATGTATTCAGTTCCCACATTATATGATTTCAATGATGAGAT
ATTCATCCCAAAGGCTTCTGCTGAGGCAAGGAGGGCTGCCAAATCTTTTGAAGGAATATCTGAATATCCTTTCGTTGAAGAGATGATGGGTGAGGAGGTTTGTCATGAAAATGAAAATGTAAATTCATCAGGACTAGGTAATTCTTATGATAATCTATCCATGAAAGGAGAAGCATCAATGGCAGTTGGGGAGAGGAAGA
GTACAAAAGATATTGAAGTGACTTCTTCTGAGCTGCCTATCGGGCTACCATCTGATGCAAGTGTGCTTTTTGAAGACAAATCCTTTGAAGGGACTCCTGTAGAGTGTGATTTCGAGGATGGAAAATCAGGAAATAAGGATGGCATCAAGCTAGGAGAACAAGGTTTCCTGCTTGAGGAACTAAGTCTTTTTTATAAGGAT
CCTCAAGGGGATATTCAGGGGCCTTTTCTAGGTATAGATATAATTCAATGGTTTGAACAAGGTTTTTTTGGTACAGATTTACTAGTGTGCCTTTCTGATGCTCCGGAGGGAACACCATTTCAGCCCCTTGGAGAGCTTATTCTACACATGAGACATAAAGCTCAATCATCTCATGGTACTGTCTTTGAGGATTCAGAAGA
CACCAAAGGAATGAGTTCAAGAAGTGGTCATGATATTTCATCTAAAAAGGCTGGTATCATATATGAACATAATTCGATACTCAATCCTGAGCAAGAAAAGACATTGTCCGACTTTTCAGAGCAGGTCAATGCAGGATTAACTGGCAGAGGACTATCTTTTCCTGATATGCTCAAAGGTACTAAGAATAATACACTTGATC
ATCAAGAACAACACTTTTTGGAATCTGCTACAGAGAAAAATGCAGAAATTCTTGCAAGAATAATTCATCATGGAATACCACCGGGAGAATCTATTGGAAGTCTTGAGAGGCAGAGAATGTCTTCCTTACCTCGGTTTGATACAGATGATGGCCTTGTTCCTTGGCAATCTGAAGCAAATGCTTTTGGTTCAGTCTGGCCT
CAGCAGGAAGCGGATCAGTTCATTGATATAAGATCCGGTCTGCTGGATGCCACAGTTAGCAGTTCCATTTCTGGTAATATGCTTTCAATGATGTCAAGGATGCAATCCCTACCTAATAATCAGCGACAAGACTTATTTCATCAATCAAATGGTGCCGGCACTTCTCCTCCAATTGATAGGGCTTGGTCTGAAACATTTCA
GAGGGATGCATATTCCACTAACAAAATTTTCAATGTTGCATCTGATCCTCATCGTATGACACATCCTGAACAGAATTTGCAAAGAATAGAGCGAGCTAACCTTTTATTTCATCACCAGTTGGAGCAACAGAAGCAGAAACAGCAACAGCAGCAGCAACAACAACAACAAATGCTGATGCAACGGCAATTGCAGTCCGGAG
CTAAATTGCTGGATCACAGTACAATTTCAGGTATGGGTCATAACTTTGACTCTTCACTGTTGCAACACCACCAATTGCTACAACCACCTTTACAACCACGTCAGCAACTACCTCCATCTGCGCATGTTATGGACCCAATCTCAAGGCTTCAGCTGCAGCATCACCATCACCAACCCCAACACCATCTACAACTACAACCA
CATCAGCAGCAAACCCAGCATAATATGCAGATGGAGCACCAACGGCAGTCTCATCAAACAGGCCTAGATCAGTTACTGTCTCATCAGATATATGATCCTAGCTATGCTCAGAATCAATTTCCTTCTGGTGGAAGTAGCATTGATCAGCTTATGTTAAGGCAAGAAGGTATTAGGCATGAATCCCAGCCACATCATCAGTC
TATACGTAGGCAAAGCAATTCAATGGACCCAAGCCTTTCATTGGAACAACTTTTGCATCAGAGAAGACATGAGCTATCAGCTAACCAGTTTCCCTTGCCATCGCAACGGCAATTTATTGATGAAAGACGTGTAAGTGGAGTTTGGGCTGTTGATGAGTCTGGTGAGTTTGTACAGACGCAAGCACCTACTCTCCATCCCT
TTGATTTATACCAGCCGCATCAGCAGATACTGAATTCACAACCACAACACACACAATTATTAAATTTTCAATCTTCAAGCACCCTAGCATCTAATTCAGAAATAAAATATCCTTACCTATTTGAGCGGGCTGAGCATCATGACCATAGCAGTGAAGGTCGATCATTGCATGGTATGCATGATCCCAACCTGTTACCTTTT
GAAAGGCCTACTGATTTGTCTTCGCTGAACATGGACTTCTCACATTCTCTTAAACAAGCCCAGGAACGCAGGGTGCAGGAAAGGTATAATGTTACACGTTTGGCAGGACAGGATGAACAATTCCCATCAGAAGTTACCTACAATTCCCGATCTCATCCAATGGCCTTCCATGGTATACAATCTGAGGTTACAAATGGGAT
TTGGGAGCAACCCTCCAGCACACATTCTACTGATCTTCATGTTCAGGGTCCTGGCTTTTTTATGCAGCAAGGACAACCTTTCGATTTTATTACTGAAAGCCAAGGTCAGAAGTTGCATCGAGTTGGGTTAAATTGGCAGTCCAATGAAGGTTCTCAAGAGCATGTTTCTTCTGAAGAATTGCACCGGAGATCTTTAATTT
CTACACACACTGCTGATTTAGAGACAAGACAATCTCATGGCGACTCGCTTCATCAAAAATTCCCTCTTCGTTCTGGTTCGTCATCTGTAATGCCTGCTATTCAAAAATCGTAGAAATGAAGATCTTTCAGAGGTTCTAGAAGATTTGGCTGGGAATGGTGTTGGATCGACTTATATGGCCTGTAACTCAGCATCTGAAAT
GCAGATGGGGACTGGCTTATCTGTAGATAGTGTATATGATGGTATGCATTCAGAAGCATTTATTGAGGAGCCATTTTATTCATCAAATAACAATGAGCTGCAACTGTCTCTGCAAGTGTCTGATTCACTCGGGCAATCCTCACGAGATGACATTGAGAGTTTGGAATTCAAAGAAGCTAAAAAAAACAAACGGCGGAGTT
CTAAAGCTAGAGGGACAGTAAAAATGACTGTTGTTTCTTCAGATGCGTCTTCAGTGTCAAACTTATCTACAGGAGCTCCAGAATTGAGTCAAAATCAACAAACAATAAAGCATGACAGTTCCACTGTGCAAGAGTTGTCTTCATGGAGTACAGATTTTAATAGACGAGGGTTATCAGTGCATACTGGCATGGACACTAAA
AAATCCCTACAGAAAACTGGGCAGTTGGTTATTGAAAGCAACAATGCAGATGGGAGAATGAGTCATTCCAGTGACAATGGTGATCAAATATCAACAGTTGAACGGGAAAATTCTAAATTTTCTGCAAAAGCTTTTGAATTGTCAAATTTGGCTTCTTCTCCCTTATCTACAGTACAGAGAAAGCTACTGAGTAACAGAAG
AGCATCTTATGACGCTGGAGAAAGCATTGCAGAAAATGCTGGTGGCAATCTTGCAGTGAAGCCTTCAGAAAGTGCTTTCCAACGGAAAGAAAATTTACCCATTTCAAACTTTAGTAAGACTTCTGCTTCCAGTGATGCTGACACAAGGAATGAAGGAGCAAAATCCTTTCTAAATACATTGAAAAATCCAAAGAAACCAG
AACCAGAAAAAGAGAAGAGCAAAGTTGATTCATCTGCAATTTTAGATTTTGATAGTCAAGGATCAAAAAGTTCAAAAAAGAAGGGCAAGAAAGGAAGGCAGATAGACCCTTCTTTGCTTGGATTTAAGGTTACAAGTAATCGCATCAATATGGGGGAGATTCAACACCCTGATGATTAAATTCCTCTAGTTGATTTGTAT
TCTTAGAGTGGTTATCATAATCCAAGGTAAATATGCCATCATTGATTTGGTTTTTAAATGGGTCATATTCTTAAATTTTTTCTCTGTACAGGTATTTTTGGCATAGTTCCCCCCTGATAGTTTTTTTTCCACTTATTATGAAATTGAATGATTCGAGTCTCTATCAAATGAATTTTCATTTGTTGCACCCAGTAATTGTT
TTCATGTCATTTTACAATGTTTTAGGGAAGACCGGAAATCTACCTTTTGTTTGAGGTGCAATTCTATTCTCTGTTGGTCTGTTCCTCTACTAACAAATTAAAGCCATTTATGCTTGTCACAGTACCTCTTGGTCCTTGCCATTGTGATCATGTTGTTATAGTAGGATTTGATTTATGTAAGTGGTTGAGTCGGTACTATT
TACAAAATATTATAATTAAGTAGAATCTCAACATGTGGCTTTTGTTAATTTTAGTACCTTTTGTTGTCAATTTATGGGACCTGTATATCTATGTTAGATGGAAAAAAGTTTATTGTACAATTTTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTTGATGATCTATGTTAGATGGAAAAATGTTTATCGTACAATT
TTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTCGATGATAACTGTCCAGATTAAATGACATGAGTCACATTATGCAGTAATTTCATGAGCTTTGGCTTTAGATGTTGTGACAGAGGAGTATCTAGTGTCAAAGCTGTCCACCCGGTAATTTTACCTGTCACATCTGCATCTTGCCTAATTTGCATGTCTGGTTAG
TTACCTAAAGGGTTGAGATCATGGGCTAAGTAAAATAATGTGTCCACATGGGCCATACACTAGAAACCTATATGTTGCCCTGGCATACTTAATGGAATAAGTTAGATGCAGCAGTAGTTGCTGCAGGACCTATGACATTGAAACCATAGACTTCCCGAAAGAGGACCAAGTCAACATACATTGATCAATTCTGTATCCAA
AACTCCAGTTACTACATTCTATGTGTTATTTCCTATGTTGCATGGGTACCTGTACCAGGCGGTCGGGTACATGAACCCGTACCGGGTACGCGGGTACTTCAATGGTGCGTTCCCAGCCGTACCCAAATTCAAACTCGCATACCGGGTACTTCACTTATGAAAACAGGGTACTTGCAT


>TRINITY_DN121844_c5_g5_i1 from sample A3 / Example unspliced isoform:
CGGATTGATTAGCGATAACATAAAATATTAATTAGCGAAAGATCGCGAGGAAGGGAAGGGTTCCAATTTCACATCTGTTGCTATCTCCATTTCCCTTCCCTCGACTTGATAAGTAGTAAGCGCATCACCTCCCTTGGTCGATTACTTTTAATTTCAGCGACATACAGCCCGATCTCTCTGCGCGCACGGACTTAAGACCC
CAGCCTTGGTATATCCCGCACTTGTTTCGCATTTGCCCTTGCTGCTGCCACTGCTTCAACCGCAAATCCCTGTCGTATCCTCGCCCTCCTTTACGAGCCATGGGTTTCATCCTTCCGAAACCCCATATGGTTTGAATTTGCATACCCCGTCCTGTTCTTTAGTAATTTCTTCCCGTACATTCGATTTTCAGGGCACAATC
TGCAAGAGTAGCGAACCTAACGAGGTAGTCGTTAAATACCCCTTGGATTTGGTTTTTGTTCGAGGCGTAAGAATGGCCGAAAGGAAGTCCGATCTTCCGCAGCATTCTGAGACGCAGGATCATCCGAATATCAAAGTGTTGTGCTTAGATGGAGACAGCTCGGGATTGTTGTCTGGTAACCCTGATGCGGAATTTAAAGG
TAGGTATATATGGTTATATCTTTTCGCTCTGTATATCCACTATACATAGTAGACACGCTTGTTTGTTGAGATGGTTCATGACACTTGTTGCAGATGTATCTGGGCCTGAAAATGTAATCCCTCTTTCTCCTCAATGGCTGCACTCAAGGGTTGGTGAGACCAAGgtatttgcaacacttcttcaatagggctttgtaaga
tttgatttattgtctctagattttacttgattgagataaagtgattacccactgcaatactgccatattcgacgctttgaattgtttattactattatttttcaatgtcttagttctaacaacatttgacaaacataatgttctgcacaacaacttcaattgataggtatatcttttgttttaactatctgtatggttgt
tgctgctatgtttttatattttgctttagGAACCGCGGACTCCAAACAGTTTTAGTCAGAGTGGAAGCACTGATGCAAATCTCAAAGAGCAATGGAGAGGAGAGGGTTCTCTTGACAAGAAAGATTGGCGGAGAACTGCTCCTGGTGAGAGCGACGGTGGTCGGCGCTGGCGTGAAGAGGAAAGGGAAAATACTGCAGTT
GCCAGAAGAGACCGTAGAAAAGAAGGGGACAGAGAAGTTGAAACTCGGAAGGTTGATCGTAGATCAGAGAATCCTTCACTAAGAGAAGTTGCAGACAATAAGCATCCAAATTCGTCAGACCGTTGGCAAGATACTACTGCCCGTGATCCAGGCCACGAGGCACGTCGTGACAGGAAATGGTCCACAGCATGGGGACCTGA
AAACAATGAAAAAGATATACGTCGAGAGAAACGCAGTGAAGTGGACAAGGAGGATGGTGGTCTTGATAGACAACCTTCTTTTTCAAGCAGTCGTCTATTGGCTGCAGACCGTGAATCGGAAAAATCATCGACTGACAAGTGGAGACCATCCAGACTCCGGGACAAACTTAAAACACAAAAGGATGGTGTTGGATTCCTTG
CAGGTCGAGGCCGTGGAAATGGTTTGACAAATAGTCTTTCGATGAGAACTCCTATTGGTGCTTCTACTATTACAAATGCTTTTTGCTACCCACGGGCCAAACTTCTTGACATTTATAGGGATTGTGGATCTTTCTCACCTTTACGAGGTGTTGCCGATGGATTTGAGGAAGCTTCTGGCGTTACTTCTTCTGACCATTTT
GAGCCATTGGCATTTGTCTCGCCTGAGCCAGAAGAGCAGGCTGTATTAGAAGACATCTCTAAGGGGAAAATTGCAGGCGGTGAAGCAATCCGCAGGACATTTAACAATACATCTGGCAGGATCAATATTGATGGCCTTGGAATTAGAGAGGAGATATTGTCCAAGAGCAATAAGAAAAGGGATAGTGATGAATCTGTGAG
CTGTGTGATGGATGATTCTCAATTAAAGGTGCATGAAGATGTGCTCCCTGATTTAGATGAGTCTGAACTTGTAGATTCAGTTTCCAAGGAGATCACAGCAGGCAGCAAAGATAAGACAATGTATTCAGTTCCCACATTATATGATTTCAATGATGAGATATTCATCCCAAAGGCTTCTGCTGAGGCAAGGAGGGCTGCCA
AATCTTTTGAAGGAATATCTGAATATCCTTTCGTTGAAGAGATGATGGGTGAGGAGGTTTGTCATGAAAATGAAAATGTAAATTCATCAGGACTAGGTAATTCTTATGATAATCTATCCATGAAAGGAGAAGCATCAATGGCAGTTGGGGAGAGGAAGAGTACAAAAGATATTGAAGTGACTTCTTCTGAGCTGCCTATC
GGGCTACCATCTGATGCAAGTGTGCTTTTTGAAGACAAATCCTTTGAAGGGACTCCTGTAGAGTGTGATTTCGAGGATGGAAAATCAGGAAATAAGGATGGCATCAAGCTAGGAGAACAAGGTTTCCTGCTTGAGGAACTAAGTCTTTTTTATAAGGATCCTCAAGGGGATATTCAGGGGCCTTTTCTAGGTATAGATAT
AATTCAATGGTTTGAACAAGGTTTTTTTGGTACAGATTTACTAGTGTGCCTTTCTGATGCTCCGGAGGGAACACCATTTCAGCCCCTTGGAGAGCTTATTCTACACATGAGACATAAAGCTCAATCATCTCATGGTACTGTCTTTGAGGATTCAGAAGACACCAAAGGAATGAGTTCAAGAAGTGGTCATGATATTTCAT
CTAAAAAGGCTGGTATCATATATGAACATAATTCGATACTCAATCCTGAGCAAGAAAAGACATTGTCCGACTTTTCAGAGCAGGTCAATGCAGGATTAACTGGCAGAGGACTATCTTTTCCTGATATGCTCAAAGGTACTAAGAATAATACACTTGATCATCAAGAACAACACTTTTTGGAATCTGCTACAGAGAAAAAT
GCAGAAATTCTTGCAAGAATAATTCATCATGGAATACCACCGGGAGAATCTATTGGAAGTCTTGAGAGGCAGAGAATGTCTTCCTTACCTCGGTTTGATACAGATGATGGCCTTGTTCCTTGGCAATCTGAAGCAAATGCTTTTGGTTCAGTCTGGCCTCAGCAGGAAGCGGATCAGTTCATTGATATAAGATCCGGTCT
GCTGGATGCCACAGTTAGCAGTTCCATTTCTGGTAATATGCTTTCAATGATGTCAAGGATGCAATCCCTACCTAATAATCAGCGACAAGACTTATTTCATCAATCAAATGGTGCCGGCACTTCTCCTCCAATTGATAGGGCTTGGTCTGAAACATTTCAGAGGGATGCATATTCCACTAACAAAATTTTCAATGTTGCAT
CTGATCCTCATCGTATGACACATCCTGAACAGAATTTGCAAAGAATAGAGCGAGCTAACCTTTTATTTCATCACCAGTTGGAGCAACAGAAGCAGAAACAGCAACAGCAGCAGCAACAACAACAACAAATGCTGATGCAACGGCAATTGCAGTCCGGAGCTAAATTGCTGGATCACAGTACAATTTCAGGTATGGGTCAT
AACTTTGACTCTTCACTGTTGCAACACCACCAATTGCTACAACCACCTTTACAACCACGTCAGCAACTACCTCCATCTGCGCATGTTATGGACCCAATCTCAAGGCTTCAGCTGCAGCATCACCATCACCAACCCCAACACCATCTACAACTACAACCACATCAGCAGCAAACCCAGCATAATATGCAGATGGAGCACCA
ACGGCAGTCTCATCAAACAGGCCTAGATCAGTTACTGTCTCATCAGATATATGATCCTAGCTATGCTCAGAATCAATTTCCTTCTGGTGGAAGTAGCATTGATCAGCTTATGTTAAGGCAAGAAGGTATTAGGCATGAATCCCAGCCACATCATCAGTCTATACGTAGGCAAAGCAATTCAATGGACCCAAGCCTTTCAT
TGGAACAACTTTTGCATCAGAGAAGACATGAGCTATCAGCTAACCAGTTTCCCTTGCCATCGCAACGGCAATTTATTGATGAAAGACGTGTAAGTGGAGTTTGGGCTGTTGATGAGTCTGGTGAGTTTGTACAGACGCAAGCACCTACTCTCCATCCCTTTGATTTATACCAGCCGCATCAGCAGATACTGAATTCACAA
CCACAACACACACAATTATTAAATTTTCAATCTTCAAGCACCCTAGCATCTAATTCAGAAATAAAATATCCTTACCTATTTGAGCGGGCTGAGCATCATGACCATAGCAGTGAAGGTCGATCATTGCATGGTATGCATGATCCCAACCTGTTACCTTTTGAAAGGCCTACTGATTTGTCTTCGCTGAACATGGACTTCTC
ACATTCTCTTAAACAAGCCCAGGAACGCAGGGTGCAGGAAAGGTATAATGTTACACGTTTGGCAGGACAGGATGAACAATTCCCATCAGAAGTTACCTACAATTCCCGATCTCATCCAATGGCCTTCCATGGTATACAATCTGAGGTTACAAATGGGATTTGGGAGCAACCCTCCAGCACACATTCTACTGATCTTCATG
TTCAGGGTCCTGGCTTTTTTATGCAGCAAGGACAACCTTTCGATTTTATTACTGAAAGCCAAGGTCAGAAGTTGCATCGAGTTGGGTTAAATTGGCAGTCCAATGAAGGTTCTCAAGAGCATGTTTCTTCTGAAGAATTGCACCGGAGATCTTTAATTTCTACACACACTGCTGATTTAGAGACAAGACAATCTCATGGC
GACTCGCTTCATCAAAAATTCCCTCTTCGTTCTGGTTCGTCATCTGTAATGCCTGGTAATGTGCTTGCTACTTCTTCTGAACAAAGCCAGTGTATGTGGACTGCTGTGAACTCAAGAGAGTGTATTTCTGGTTGTACTTATGATTCTTCTGATTTGATTTCAAGCCAGCTATTCAAAAATCGTAGAAATGAAGATCTTTC
AGAGGTTCTAGAAGATTTGGCTGGGAATGGTGTTGGATCGACTTATATGGCCTGTAACTCAGCATCTGAAATGCAGATGGGGACTGGCTTATCTGTAGATAGTGTATATGATGGTATGCATTCAGAAGCATTTATTGAGGAGCCATTTTATTCATCAAATAACAATGAGCTGCAACTGTCTCTGCAAGTGTCTGATTCAC
TCGGGCAATCCTCACGAGATGACATTGAGAGTTTGGAATTCAAAGAAGCTAAAAAAAACAAACGGCGGAGTTCTAAAGCTAGAGGGACAGTAAAAATGACTGTTGTTTCTTCAGATGCGTCTTCAGTGTCAAACTTATCTACAGGAGCTCCAGAATTGAGTCAAAATCAACAAACAATAAAGCATGACAGTTCCACTGTG
CAAGAGTTGTCTTCATGGAGTACAGATTTTAATAGACGAGGGTTATCAGTGCATACTGGCATGGACACTAAAAAATCCCTACAGAAAACTGGGCAGTTGGTTATTGAAAGCAACAATGCAGATGGGAGAATGAGTCATTCCAGTGACAATGGTGATCAAATATCAACAGTTGAACGGGAAAATTCTAAATTTTCTGCAAA
AGCTTTTGAATTGTCAAATTTGGCTTCTTCTCCCTTATCTACAGTACAGAGAAAGCTACTGAGTAACAGAAGAGCATCTTATGACGCTGGAGAAAGCATTGCAGAAAATGCTGGTGGCAATCTTGCAGTGAAGCCTTCAGAAAGTGCTTTCCAACGGAAAGAAAATTTACCCATTTCAAACTTTAGTAAGACTTCTGCTT
CCAGTGATGCTGACACAAGGAATGAAGGAGCAAAATCCTTTCTAAATACATTGAAAAATCCAAAGAAACCAGAACCAGAAAAAGAGAAGAGCAAAGTTGATTCATCTGCAATTTTAGATTTTGATAGTCAAGGATCAAAAAGTTCAAAAAAGAAGGGCAAGAAAGGAAGGCAGATAGACCCTTCTTTGCTTGGATTTAAG
GTTACAAGTAATCGCATCAATATGGGGGAGATTCAACACCCTGATGATTAAATTCCTCTAGTTGATTTGTATTCTTAGAGTGGTTATCATAATCCAAGGTAAATATGCCATCATTGATTTGGTTTTTAAATGGGTCATATTCTTAAATTTTTTCTCTGTACAGGTATTTTTGGCATAGTTCCCCCCTGATAGTTTTTTTT
CCACTTATTATGAAATTGAATGATTCGAGTCTCTATCAAATGAATTTTCATTTGTTGCACCCAGTAATTGTTTTCATGTCATTTTACAATGTTTTAGGGAAGACCGGAAATCTACCTTTTGTTTGAGGTGCAATTCTATTCTCTGTTGGTCTGTTCCTCTACTAACAAATTAAAGCCATTTATGCTTGTCACAGTACCTC
TTGGTCCTTGCCATTGTGATCATGTTGTTATAGTAGGATTTGATTTATGTAAGTGGTTGAGTCGGTACTATTTACAAAATATTATAATTAAGTAGAATCTCAACATGTGGCTTTTGTTAATTTTAGTACCTTTTGTTGTCAATTTATGGGACCTGTATATCTATGTTAGATGGAAAAAAGTTTATTGTACAATTTTTTTC
TAGAGGCTGTGTTAGTGGTCAGTAAACTGGTTGATGATCTATGTTAGATGGAAAAATGTTTATCGTACAATTTTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTCGATGATAACTGTCCAGATTAAATGACATGAGTCACATTATGCAGTAATTTCATGAGCTTTGGCTTTAGATGTTGTGACAGAGGAGTATCT
AGTGTCAAAGCTGTCCACCCGGTAATTTTACCTGTCACATCTGCATCTTGCCTAATTTGCATGTCTGGTTAGTTACCTAAAGGGTTGAGATCATGGGCTAAGTAAAATAATGTGTCCACATGGGCCATACACTAGAAACCTATATGTTGCCCTGGCATACTTAATGGAATAAGTTAGATGCAGCAGTAGTTGCTGCAGGA
CCTATGACATTGAAACCATAGACTTCCCGAAAGAGGACCAAGTCAACATACATTGATCAATTCTGTATCCAAAACTCCAGTTACTACATTCTATGTGTTATTTCCTATGTTGCATGGGTACCTGTACCAGGCGGTCGGGTACATGAACCCGTACCGGGTACGCGGGTACTTCAATGGTGCGTTCCCAGCCGTACCCAAAT
TCAAACTCGCATACCGGGTACTTCACTTATGAAAACAGGGTACTTGCAT



Intron 8 GTATGGTTGT ... TGCTTTTCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A123.0187026
A214.4383014
A32072018
A418.8182019
A59.094004
A60300
A79.382903
A80200
A918.751303
A1016.3951010



>TRINITY_DN110315_c0_g1_i9 from sample A5 / Example spliced isoform
GGCGGATTGATTAGCGATAACATAAAATATTAATTAGCGAAAGATCGCGAGGAAGGGAAGGGTTCCAATTTCACATCTGTTGCTATCTCCATTTCCCTTCCCTCGACTTGATAAGTAGTAAGCGCATCACCTCCCTTGGTCGATTACTTTTAATTTCAGCGACATACAGCCCGATCTCTCTGCGCGCACGGACTTAAGAC
CCCAGCCTTGGTATATCCCGCACTTGTTTCGCATTTGCCCTTGCTGCTGCCACTGCTTCAACTGCAAATCCCTGTCGTATCCTCGCCCTCCTTTACGAGCCATGGGTTTCATCCTTCCGAAACCCCATATGGTTTGAATTTGCATACCCCCTCCTGTTCTTCAGTAATTTCTTCCCGTACATTCGATTTTCAGGGCACAA
TCTGCAAGAGTAGCGAACCAGAGGTAGTCGTTAAATACCCCTTGGATTTGGTTTTTGTTCGAGGCGTAAGAATGGCCGAAAGGAAGTCCGATCTTCCGCAGCATTCTGAGACGCAGGATCATCCGAATATCAAAGTGTTGTGCTTAGATGGAGACAGCTCGGGATTGTTGTCTGGTAACCCTGATGCGGAATTTAAAGAT
GTATCTGGGCCTGAAAATGTAATCCCTCTTTCTCCTCAATGGCTGCACTCAAGGGTTGGTGAGACCAAGGAACCGCGGACTCCAAACAGTTTTAGTCAGAGTGGAAGCACTGATGCAAATCTCAAAGAGCAATGGAGAGGGGAGGGTTCTCTTGACAAGAAAGATTGGCGGAGAACTGCTCCTGGTGAGAGCGACGGTGG
TCGGCGCTGGCGTGAAGAGGAAAGGGAAAATACTGCAGTTGCCAGAAGAGACCGTAGAAAAGAAGGGGACAGAGAAGTTGAAACTCGGAAGGTTGATCGTAGATCAGAGAATCCTTCACTAAGAGAAGTTGCAGACAATAAGCATCCAAATTCGTCAGACCGTTGGCAAGATACTACTGCCCGTGATCCAGGCCACGAGG
CACGTCGTGACAGGAAATGGTCCACAGCATGGGGACCTGAAAACAATGAAAAAGATATACGTCGAGAGAAACGCAGTGAAGTGGACAAGGAGGATGGTGGTCTTGATAGACAACCTTCTTTTTCAAGCAGTCGTCTATTGGCTGCAGACCGTGAATCGGAGAAATCATCGACTGACAAGTGGAGACCATCCAGACTCCGG
GACAAACTTAAAACACAAAAGGATGGTGTTGGATTCCTTGCAGGTCGAGGCCGTGGAAATGGTTTGACAAATAGTCTTTCGATGAGAACTCCTATTGGTGCTTCTACTATTACAAATGCTTTTTGCTACCCACGGGCCAAACTTCTTGACATTTATAGGGATTGTGGATCTTTCTCACCTTTACGAGGTGTTGCCGATGG
ATTTGAGGAAGCTTCTGGCGTTACTTCTTCTGACCATTTTGAGCCATTGGCATTTGTCTCGCCTGAGCCAGAAGAGCAGGCTGTATTAGAAGACATTTCTAAGGGGAAAATTGCAGGCGGTGAAGCAATCCGCAGGACATTTAACAATACATCTGGCAGGATCAATATTGATGGCCTTGGAATTAGAGAGGAGATATTGT
CCAAGAGCAATAAGAAAAGGGATAGTGATGAATCTGTGAGCTGTGTGATGGATGATTCTCAATTAAAGGTGCATGAAGATGTGCTCCCTGATTTAGATGAGTCTGAACTTGTAGATTCAGTTTCCAAGGAGATCACAGCAGGCAGCAAAGCAATGTATTCAGTTCCCACATTATATGATTTCAATGATGAGATATTCATC
CCAAAGGCTTCTGCTGAGGCAAGGAGGGCTGCCAAATCTTTTGAAGGAATATCTGAATATCCTTTCGTTGAAGAGATGATGGGTGAGGAGGTTTGTCATGAAAATGAAAATGTAAATTCATCAGGACTAGGTAATTCTTATGATAATCTATCCATGAAAGGAGAAGCATCAATGGCAGTTGGGGAGAGGAAGAGTACAAA
AGATATTGAAGTGACTTCTTCTGAGCTGCCTATCGGGCTACCATCTGATGCAAGTGTGCTTTTTGAAGACAAATCCTTTGAAGGGACTCCTGTAGAGTGTGATTTCGAGGATGGAAAATCAGGAAATAAGGATGGCATCAAGCTAGGAGAACAAGGTTTCCTGCTTGAGGAACTAAGTCTTTTTTATAAGGATCCTCAAG
GGGATATTCAGGGGCCTTTTCTAGGTATAGATATAATTCAATGGTTTGAACAAGGTTTTTTTGGTACAGATTTACTAGTGTGCCTTTCTGATGCTCCGGAGGGAACACCATTTCAGCCCCTTGGAGAGCTTATTCTACACATGAGACATAAAGCTCAATCATCTCATGGTACTGTCTTTGAGGATTCAGAAGACACCAAA
GGAATGAGTTCAAGAAGTGGTCATGATATTTCATCTAAAAAGGCTGGTATCATATATGAACATAATTCAATACTCAATCCTGAGCAAGAAAAGACATTGTCCGACTTTTCAGAGCAGGTCAATGCAGGATTAACTGGCAGAGGACTATCTTTTCCTGATATGCTCAAAGGTACTAAGAATAATACACTTGATCATCAAGA
ACAACACTTTTTGGAATCTGCTACAGAGAAAAATGCAGAAATTCTTGCAAGAATAATTCATCATGGAATACCACCGGGAGAATCTATTGGAAGTCTTGAGAGGCAGAGAATGTCTTCCTTACCTCGGTTTGATACAGATGATGGCCTTGTTCCTTGGCAATCTGAAGCAAATGCTTTTGGTTCAGTCTGGCCTCAGCAGG
AAGCGGATCAGTTCATTGATATAAGATCCGGTCTGCTGGATGCCACAGTTAGCAGTTCCATTTCTGGTAATATGCTTTCAATGATGTCAAGGATGCAATCCCTACCTAATAATCAGCGACAAGACGTATTTCATCAATCAAATGGTGCCGGCACTTCTCCTCCAATTGATAGGGCTTGGTCTGAAACATTTCAGAGGGAT
GCATATTCCACTAACAAAATTTTCAATGTTGCATCTGATCCTCATCGTATGACACATCCTGAACAGAATTTGCAAAGAATAGAGCGAGCTAACCTTTTATTTCATCACCAGTTGGAGCAACAGAAGCAGAAACAGCAACAGCAGCAGCAACAACAACAACAAATGCTGATGCAACGGCAATTGCAGTCCGGAGCTAAATT
GCTGGATCACAGTACAATTTCAGGTATGGGTCATAACTTTGACTCTTCACTGTTGCAACACCACCAATTGCTACAACCACCTTTACAACCACGTCAGCAACTACCTCCATCTGCGCATGTTATGGACCCAATCTCAAGGCTTCAGCTGCAGCATCACCATCACCAACCCCAACACCATCTACAACTACAACCACATCAGC
AGCAAACCCAGCATAATATGCAGATGGAGCACCAACGGCAGTCTCATCAAACAGGCCTAGATCAGTTACTGTCTCATCAGATATATGATCCTAGCTATGCTCAGAATCAATTTCCTTCTGGTGGAAGTAGCATTGATCAGCTTATGTTAAGGCAAGAAGGTATTAGGCATGAATCCCAGCCACATCATCAGTCTATACGT
AGGCAAAGCAATTCAATGGACCCAAGCCTTTCATTGGAACAACTTTTGCATCAGAGAAGACATGAGCTATCAGCTAACCAGTTTCCCTTGCCATCGCAACGGCAATTTATTGATGAAAGACGTGTAAGTGGAGTTTGGGCTGTTGATGAGTCTGGTGAGTTTGTACAGACGCAAGCACCTACTCTCCATCCCTTTGATTT
ATACCAGCCGCATCAGCAGATACTGAATTCACAACCACAACACACACAATTATTAAATTTTCAATCTTCAAGCACCCTAGCATCTAATGCAGAAATAAAATATCCTTACCTATTTGAGCGGGCTGAGCATCATGACCATAGCAGTGAAGGTCGATCATTGCATGGTATGCATGATCCCAACCTGTTACCTTTTGAAAGGC
CTACTGATTTGTCTTCGCTGAACATGGACTTCTCACATTCTCTTAAACAAGCCCAGGAACGCAGGGTGCAGGAAAGGTATAATGTTACACGTTTGGCAGGACAGGATGAACAATTCCCATCAGAAGTTACCTACAATTCCCGATCTCATCCAATGGCCTTCCATGGTATACAATCTGAGGTTACAAATGGGATTTGGGAG
CAACCCTCCAGCACACATTCTACTGATCTTCATGTTCAGGGTCCTGGCTTTTTTATGCAGCAAGGACAACCTTTCGATTTTATTACTGAAAGCCAAGGTCAGAAGTTGCATCGAGTTGGGTTAAATTGGCAGTCCAATGAAGGTTCTCAAGAGCATGTTTCTTCTGAAGAATTGCACCGGAGATCTTTAATTTCTACACA
CACTGCTGATTTAGAGACAAGACAATCTCATGGCGACTCGCTTCATCAAAAATTCCCTCTTCGTTCTGGTTCGTCATCTGTAATGCCTGGTAATGTGCTTGCTACTTCTTCTGAACAAAGCCAGTGTATGTGGACTGCTGTGAACTCAAGAGAGTCTATTTCTGGTTGTACTTATGATTCTTCTGATTTGATTTCAAGCC
AGCTATTCAAAAATCGTAGAAATGAAGATCTTTCAGAGGTTCTAGAAGATTTGGCTGGGAATGGTGTTGGATCGACTTATATGGCCTGTAACTCAGCATCTGAAATGCAGATGGGGACTGGCTTATCTGTAGATAGTGTATATGATGGTATGCATTCAGAAGCATTTATTGAGGAGCCATTTTATTCATCAAATAACAAT
GAGCTGCAACTGTCTCTGCAAGTGTCTGATTCACTCGGGCAATCCTCACGAGATGACATTGAGAGTTTGGAATTCAAAGAAGCTAAAAAAAACAAACGGCGGAGTTCTAAAGCTAGAGGGACAGTAAAAATGACTGTTGTTTCTTCAGATGCGTCTTCAGTGTCAAACTTATCTACAGGAGCTCCAGAATTGAGTCAAAA
TCAACAAACAATAAAGCATGACAGTTCCACTGTGCAAGAGTTGTCTTCATGGAGTACAGATTTTAATAGACGAGGGTTATCAGTGCATACTGGCATGGACACTAAAAAATCCCTACAGAAAACTGGGCAGTTGGTTATTGAAAGCAACAATGCAGATGGGAGAATGAGGCATTCCAGTGACAATGGTGATCAAATATCAA
CAGTTGAACGGGAAAATTCTAAATTTTCTGCAAAAGCTTTTGAATTGTCAAATTTGGCTTCTTCTCCCTTATCTACAGTACAGAGAAAGCTACTGAGTAACAGAAGAGCATCTTATGACGCTGGAGAAAGCATTGCAGAAAATGCTGGTGGCAATCTTGCAGTGAAGCCTTCAGAAAGTGCTTTCCAACGGAAAGAAAAT
CTACCCATTTCAAACTTTAGTAAGACTTCTGCTTCCAGTGATGCTGACACAAGGAATGAAGGAGCAAAATCCTTTCTAAATACATTGAAAAATCCAAAGAAACCAGAACCAGAAAAAGAGAAGAGCAAAGTTGATTCATCTGCAATTTTAGATTTTGATAGTCAAGGATCAAAAAGTTCAAAAAAGAAGGGCAAGAAAGG
AAGGCAGATAGACCCTTCTTTGCTTGGATTTAAGGTTACAAGTAATCGCATCAATATGGGGGAGATTCAACACCCTGATGATTAAATTCCTCTAGTTGATTTGTATTCTTAGAGTGGTTATCATAATCCAAGGTAAATATGCC


>TRINITY_DN110315_c0_g1_i8 from sample A5 / Example unspliced isoform:
CTCGCCTGAGCCAGAAGAGCAGgtatggttgtaatgttctgtttttctatgggattattatttatctttatgtggacttaataattgtgctagaatatctttcaatatctaaagtgttgttttgaactcgaagatttaagaaactttgtgggcatgatttttttaaagaaatatgtgattattgcctgtatatctattcc
atatggcttgttgatcagagaacaattatgatccatgctttcctctgctggcatgcttttcagGCTGTATTAGAAGACATTTCTAAGGGGAAAATTGCAGGCGGTGAAGCAATCCGCAGGACATTTAACAATACATCTGGCAGGATCAATATTGATGGCCTTGGAATTAGAGAGGAGATATTGTCCAAGAGCAATAAGAA
AAGGGATAGTGATGAATCTGTGAGCTGTGTGATGGATGATTCTCAATTAAAGGTGCATGAAGATGTGCTCCCTGATTTAGATGAGTCTGAACTTGTAGATTCAGTTTCCAAGGAGATCACAGCAGGCAGCAAAGCAATGTATTCAGTTCCCACATTATATGATTTCAATGATGAGATATTCATCCCAAAGGCTTCTGCTG
AGGCAAGGAGGGCTGCCAAATCTTTTGAAGGAATATCTGAATATCCTTTCGTTGAAGAGATGATGGGTGAGGAGGTTTGTCATGAAAATGAAAATGTAAATTCATCAGGACTAGGTAATTCTTATGATAATCTATCCATGAAAGGAGAAGCATCAATGGCAGTTGGGGAGAGGAAGAGTACAAAAGATATTGAAGTGACT
TCTTCTGAGCTGCCTATCGGGCTACCATCTGATGCAAGTGTGCTTTTTGAAGACAAATCCTTTGAAGGGACTCCTGTAGAGTGTGATTTCGAGGATGGAAAATCAGGAAATAAGGATGGCATCAAGCTAGGAGAACAAGGTTTCCTGCTTGAGGAACTAAGTCTTTTTTATAAGGATCCTCAAGGGGATATTCAGGGGCC
TTTTCTAGGTATAGATATAATTCAATGGTTTGAACAAGGTTTTTTTGGTACAGATTTACTAGTGTGCCTTTCTGATGCTCCGGAGGGAACACCATTTCAGCCCCTTGGAGAGCTTATTCTACACATGAGACATAAAGCTCAATCATCTCATGGTACTGTCTTTGAGGATTCAGAAGACACCAAAGGAATGAGTTCAAGAA
GTGGTCATGATATTTCATCTAAAAAGGCTGGTATCATATATGAACATAATTCAATACTCAATCCTGAGCAAGAAAAGACATTGTCCGACTTTTCAGAGCAGGTCAATGCAGGATTAACTGGCAGAGGACTATCTTTTCCTGATATGCTCAAAGGTACTAAGAATAATACACTTGATCATCAAGAACAACACTTTTTGGAA
TCTGCTACAGAGAAAAATGCAGAAATTCTTGCAAGAATAATTCATCATGGAATACCACCGGGAGAATCTATTGGAAGTCTTGAGAGGCAGAGAATGTCTTCCTTACCTCGGTTTGATACAGATGATGGCCTTGTTCCTTGGCAATCTGAAGCAAATGCTTTTGGTTCAGTCTGGCCTCAGCAGGAAGCGGATCAGTTCAT
TGATATAAGATCCGGTCTGCTGGATGCCACAGTTAGCAGTTCCATTTCTGGTAATATGCTTTCAATGATGTCAAGGATGCAATCCCTACCTAATAATCAGCGACAAGACGTATTTCATCAATCAAATGGTGCCGGCACTTCTCCTCCAATTGATAGGGCTTGGTCTGAAACATTTCAGAGGGATGCATATTCCACTAACA
AAATTTTCAATGTTGCATCTGATCCTCATCGTATGACACATCCTGAACAGAATTTGCAAAGAATAGAGCGAGCTAACCTTTTATTTCATCACCAGTTGGAGCAACAGAAGCAGAAACAGCAACAGCAGCAGCAACAACAACAACAAATGCTGATGCAACGGCAATTGCAGTCCGGAGCTAAATTGCTGGATCACAGTACA
ATTTCAGGTATGGGTCATAACTTTGACTCTTCACTGTTGCAACACCACCAATTGCTACAACCACCTTTACAACCACGTCAGCAACTACCTCCATCTGCGCATGTTATGGACCCAATCTCAAGGCTTCAGCTGCAGCATCACCATCACCAACCCCAACACCATCTACAACTACAACCACATCAGCAGCAAACCCAGCATAA
TATGCAGATGGAGCACCAACGGCAGTCTCATCAAACAGGCCTAGATCAGTTACTGTCTCATCAGATATATGATCCTAGCTATGCTCAGAATCAATTTCCTTCTGGTGGAAGTAGCATTGATCAGCTTATGTTAAGGCAAGAAGGTATTAGGCATGAATCCCAGCCACATCATCAGTCTATACGTAGGCAAAGCAATTCAA
TGGACCCAAGCCTTTCATTGGAACAACTTTTGCATCAGAGAAGACATGAGCTATCAGCTAACCAGTTTCCCTTGCCATCGCAACGGCAATTTATTGATGAAAGACGTGTAAGTGGAGTTTGGGCTGTTGATGAGTCTGGTGAGTTTGTACAGACGCAAGCACCTACTCTCCATCCCTTTGATTTATACCAGCCGCATCAG
CAGATACTGAATTCACAACCACAACACACACAATTATTAAATTTTCAATCTTCAAGCACCCTAGCATCTAATGCAGAAATAAAATATCCTTACCTATTTGAGCGGGCTGAGCATCATGACCATAGCAGTGAAGGTCGATCATTGCATGGTATGCATGATCCCAACCTGTTACCTTTTGAAAGGCCTACTGATTTGTCTTC
GCTGAACATGGACTTCTCACATTCTCTTAAACAAGCCCAGGAACGCAGGGTGCAGGAAAGGTATAATGTTACACGTTTGGCAGGACAGGATGAACAATTCCCATCAGAAGTTACCTACAATTCCCGATCTCATCCAATGGCCTTCCATGGTATACAATCTGAGGTTACAAATGGGATTTGGGAGCAACCCTCCAGCACAC
ATTCTACTGATCTTCATGTTCAGGGTCCTGGCTTTTTTATGCAGCAAGGACAACCTTTCGATTTTATTACTGAAAGCCAAGGTCAGAAGTTGCATCGAGTTGGGTTAAATTGGCAGTCCAATGAAGGTTCTCAAGAGCATGTTTCTTCTGAAGAATTGCACCGGAGATCTTTAATTTCTACACACACTGCTGATTTAGAG
ACAAGACAATCTCATGGCGACTCGCTTCATCAAAAATTCCCTCTTCGTTCTGGTTCGTCATCTGTAATGCCTGGTAATGTGCTTGCTACTTCTTCTGAACAAAGCCAGTGTATGTGGACTGCTGTGAACTCAAGAGAGTCTATTTCTGGTTGTACTTATGATTCTTCTGATTTGATTTCAAGCCAGCTATTCAAAAATCG
TAGAAATGAAGATCTTTCAGAGGTTCTAGAAGATTTGGCTGGGAATGGTGTTGGATCGACTTATATGGCCTGTAACTCAGCATCTGAAATGCAGATGGGGACTGGCTTATCTGTAGATAGTGTATATGATGGTATGCATTCAGAAGCATTTATTGAGGAGCCATTTTATTCATCAAATAACAATGAGCTGCAACTGTCTC
TGCAAGTGTCTGATTCACTCGGGCAATCCTCACGAGATGACATTGAGAGTTTGGAATTCAAAGAAGCTAAAAAAAACAAACGGCGGAGTTCTAAAGCTAGAGGGACAGTAAAAATGACTGTTGTTTCTTCAGATGCGTCTTCAGTGTCAAACTTATCTACAGGAGCTCCAGAATTGAGTCAAAATCAACAAACAATAAAG
CATGACAGTTCCACTGTGCAAGAGTTGTCTTCATGGAGTACAGATTTTAATAGACGAGGGTTATCAGTGCATACTGGCATGGACACTAAAAAATCCCTACAGAAAACTGGGCAGTTGGTTATTGAAAGCAACAATGCAGATGGGAGAATGAGGCATTCCAGTGACAATGGTGATCAAATATCAACAGTTGAACGG



Intron 9 GTAATGTGCT ... TTCAAGCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A167.1261121128
A254.92719380
A362.285310867
A463.825510292
A561.9204223
A668.75153729
A769.2755128120
A860.256389102
A962.215884107
A1056.78435855



>TRINITY_DN121285_c1_g2_i5 from sample A2 / Example spliced isoform
GGATTGATTAGCGATAACATAAAATATTAATTAGCGAAAGATCGCGAGGAAGGGAAGGGTTCCAATTTCACATCTGTTGCTATCTCCATTTCCCTTCCCTCGACTTGATAAGTAGTAAGCGCATCACCTCCCTTGGTCGATTACTTTTAATTTCAGCGACATACAGCCCGATCTCTCTGCGCGCACGGACTTAAGACCCC
AGCCTTGGTATATCCCGCACTTGTTTCGCATTTGCCCTTGCTGCTGCCACTGCTTCAACCGCAAATCCCTGTCGTATCCTCGCCCTCCTTTACGAGCCATGGGTTTCATCCTTCCGAAACCCCATATGGTTTGAATTTGCATACCCCGTCCTGTTCTTTAGTAATTTCTTCCCGTACATTCGATTTTCAGGGCACAATCT
GCAAGAGTAGCGAACCTAACGAGGTAGTCGTTAAATACCCCTTGGATTTGGTTTTTGTTCGAGGCGTAAGAATGGCCGAAAGGAAGTCCGATCTTCCGCAGCATTCTGAGACGCAGGATCATCCGAATATCAAAGTGTTGTGCTTAGATGGAGACAGCTCGGGATTGTTGTCTGGTAACCCTGATGCGGAATTTAAAGAT
GTATCTGGGCCTGAAAATGTAATCCCTCTTTCTCCTCAATGGCTGCACTCAAGGGTTGGTGAGACCAAGGAACCGCGGACTCCAAACAGTTTTAGTCAGAGTGGAAGCACTGATGCAAATCTCAAAGAGCAATGGAGAGGAGAGGGTTCTCTTGACAAGAAAGATTGGCGGAGAACTGCTCCTGGTGAGAGCGACGGTGG
TCGGCGCTGGCGTGAAGAGGAAAGGGAAAATACTGCAGTTGCCAGAAGAGACCGTAGAAAAGAAGGGGACAGAGAAGTTGAAACTCGGAAGGTTGATCGTAGATCAGAGAATACTTCACTAAGAGAAGTTGCAGACAATAAGCATCCAAATTCGTCAGACCGTTGGCAAGATACTACTGCCCGTGATCCAGGCCACGAGG
CACGTCGTGACAGGAAATGGTCCACAGCATGGGGACCTGAAAACAATGAAAAAGATATACGTCGAGAGAAACGCAGTGAAGTGGACAAGGAGGATGGTGGTCTTGATAGACAACCTTCTTTTTCAAGCAGTCGTCTATTGGCTGCAGACCGTGAATCGGAAAAATCATCGACTGACAAGTGGAGACCATCCAGACTCCGG
GACAAACTTAAAACACAAAAGGATGGTGTTGGATTCCTTGCAGGTCGAGGCCGTGGAAATGGTTTGACAAATAGTCTTTCGATGAGAACTCCTATTGGTGCTTCTACTATTACAAATGCTTTTTGCTACCCACGGGCCAAACTTCTTGACATTTATAGGGATTGTGGATCTTTCTCACCTTTACGAGGTGTTGCCGATGG
ATTTGAGGAAGCTTCTGGCGTTACTTCTTCTGACCATTTTGAGCCATTGGCATTTGTCTCGCCTGAGCCAGAAGAGCAGGCTGTATTAGAAGACATTTCTAAGGGGAAAATTGCAGGCGGTGAAGCAATCCGCAGGACATTTAACAATACATCTGGCAGGATCAATATTGATGGCCTTGGAATTAGAGAGGAGATATTGT
CCAAGAGCAATAAGAAAAGGGATAGTGATGAATCTGTGAGCTGTGTGATGGATGATTCTCAATTAAAGGTGCATGAAGATGTGCTCCCTGATTTAGATGAGTCTGAACTTGTAGATTCAGTTTCCAAGGAGATCACAGCAGGCAGCAAAGATAAGACAATGTATTCAGTTCCCACATTATATGATTTCAATGATGAGATA
TTCATCCCAAAGGCTTCTGCTGAGGCAAGGAGGGCTGCCAAATCTTTTGAAGGAATATCTGAATATCCTTTCGTTGAAGAGATGATGGGTGAGGAGGTTTGTCATGAAAATGAAAATGTAAATTCATCAGGACTAGGTAATTCTTATGATAATCTATCCATGAAAGGAGAAGCATCAATGGCAGTTGGGGAGAGGAAGAG
TACAAAAGATATTGAAGTGACTTCTTCTGAGCTGCCTATCGGGCTACCATCTGATGCAAGTGTGCTTTTTGAAGACAAATCCTTTGAAGGGACTCCTGTAGAGTGTGATTTCGAGGATGGAAAATCAGGAAATAAGGATGGCATCAAGCTAGGAGAACAAGGTTTCCTGCTTGAGGAACTAAGTCTTTTTTATAAGGATC
CTCAAGGGGATATTCAGGGGCCTTTTCTAGGTATAGATATAATTCAATGGTTTGAACAAGGTTTTTTTGGTACAGATTTACTAGTGTGCCTTTCTGATGCTCCGGAGGGAACACCATTTCAGCCCCTTGGAGAGCTTATTCTACACATGAGACATAAAGCTCAATCATCTCATGGTACTGTCTTTGAGGATTCAGAAGAC
ACCAAAGGAATGAGTTCAAGAAGTGGTCATGATATTTCATCTAAAAAGGCTGGTATCATATATGAACATAATTCAATACTCAATCCTGAGCAAGAAAAGACATTGTCCGACTTTTCAGAGCAGGTCAATGCAGGATTAACTGGCAGAGGACTATCTTTTCCTGATATGCTCAAAGGTACTAAGAATAATACACTTGATCA
TCAAGAACAACACTTTTTGGAATCTGCTACAGAGAAAAATGCAGAAATTCTTGCAAGAATAATTCATCATGGAATACCACCGGGAGAATCTATTGGAAGTCTTGAGAGGCAGAGAATGTCTTCCTTACCTCGGTTTGATACAGATGATGGCCTTGTTCCTTGGCAATCTGAAGCAAATGCTTTCGGTTCAGTCTGGCCTC
AGCAGGAAGCGGATCAGTTCATTGATATAAGATCCGGTCTGCTGGATGCCACAGTTAGCAGTTCCATTTCTGGTAATATGCTTTCAATGATGTCAAGGATGCAATCCCTACCTAATAATCAGCGACAAGACTTATTTCATCAATCAAATGGTGCCGGCACTTCTCCTCCAATTGATAGGGCTTGGTCTGAAACATTTCAG
AGGGATGCATATTCCACTAACAAAATTTTCAATGTTGCATCTGATCCTCATCGTATGACACATCCTGAACAGAATTTGCAAAGAATAGAGCGAGCTAACCTTTTATTTCATCACCAGTTGGAGCAACAGAAGCAGAAACAGCAACAGCAGCAGCAACAACAACAACAAATGCTGATGCAACGGCAATTGCAGTCCGGAGC
TAAATTGCTGGATCACAGTACAATTTCAGGTATGGGTCATAACTTTGACTCTTCACTGTTGCAACACCACCAATTGCTACAACCACCTTTACAACCACGTCAGCAACTACCTCCATCTGCGCATGTTATGGACCCAATCTCAAGGCTTCAGCTGCAGCATCACCATCACCAACCCCAACACCATCTACAACTACAACCAC
ATCAGCAGCAAACCCAGCATAATATGCAGATGGAGCACCAACGGCAGTCTCATCAAACAGGCCTAGATCAGTTACTGTCTCATCAGATATATGATCCTAGCTATGCTCAGAATCAATTTCCTTCTGGTGGAAGTAGCATTGATCAGCTTATGTTAAGGCAAGAAGGTATTAGGCATGAATCCCAGCCACATCATCAGTCT
ATACGTAGGCAAAGCAATTCAATGGACCCAAGCCTTTCATTGGAACAACTTTTGCATCAGAGAAGACATGAGCTATCAGCTAACCAGTTTCCCTTGCCATCGCAACGGCAATTTATTGATGAAAGACGTGTAAGTGGAGTTTGGGCTGTTGATGAGTCTGGTGAGTTTGTACAGACGCAAGCACCTACTCTCCATCCCTT
TGATTTATACCAGCCGCATCAGCAGATACTGAATTCACAACCACAACACACACAATTATTAAATTTTCAATCTTCAAGCACCCTAGCATCTAATGCAGAAATAAAATATCCTTACCTATTTGAGCGGGCTGAGCATCATGACCATAGCAGTGAAGGTCGATCATTGCATGGTATGCATGATCCCAACCTGTTACCTTTTG
AAAGGCCTACTGATTTGTCTTCGCTGAACATGGACTTCTCACATTCTCTTAAACAAGCCCAGGAACGCAGGGTGCAGGAAAGGTATAATGTTACACGTTTGGCAGGACAGGATGAACAATTCCCATCAGAAGTTACCTACAATTCCCGATCTCATCCAATGGCCTTCCATGGTATACAATCTGAGGTTACAAATGGGATT
TGGGAGCAACCCTCCAGCACACATTCTACTGATCTTCATGTTCAGGGTCCTGGCTTTTTTATGCAGCAAGGACAACCTTTCGATTTTATTACTGAAAGCCAAGGTCAGAAGTTGCATCGAGTTGGGTTAAATTGGCAGTCCAATGAAGGTTCTCAAGAGCATGTTTCTTCTGAAGAATTGCACCGGAGATCTTTAATTTC
TACACACACTGCTGATTTAGAGACAAGACAATCTCATGGCGACTCGCTTCATCAAAAATTCCCTCTTCGTTCTGGTTCGTCATCTGTAATGCCTGCTATTCAAAAATCGTAGAAATGAAGATCTTTCAGAGGTTCTAGAAGATTTGGCTGGGAATGGTGTTGGATCGACTTATATGGCCTGTAACTCAGCATCTGAAATG
CAGATGGGGACTGGCTTATCTGTAGATAGTGTATATGATGGTATGCATTCAGAAGCATTTATTGAGGAGCCATTTTATTCATCAAATAACAATGAGCTGCAACTGTCTCTGCAAGTGTCTGATTCACTCGGGCAATCCTCACGAGATGACATTGAGAGTTTGGAATTCAAAGAAGCTAAAAAAAACAAACGGCGGAGTTC
TAAAGCTAGAGGGACAGTAAAAATGACTGTTGTTTCTTCAGATGCGTCTTCAGTGTCAAACTTATCTACAGGAGCTCCAGAATTGAGTCAAAATCAACAAACAATAAAGCATGACAGTTCCACTGTGCAAGAGTTGTCTTCATGGAGTACAGATTTTAATAGACGAGGGTTATCAGTGCATACTGGCATGGACACTAAAA
AATCCCTACAGAAAACTGGGCAGTTGGTTATTGAAAGCAACAATGCAGATGGGAGAATGAGTCATTCCAGTGACAATGGTGATCAAATATCAACAGTTGAACGGGAAAATTCTAAATTTTCTGCAAAAGCTTTTGAATTGTCAAATTTGGCTTCTTCTCCCTTATCTACAGTACAGAGAAAGCTACTGAGTAACAGAAGA
GCATCTTATGACGCTGGAGAAAGCATTGCAGAAAATGCTGGTGGCAATCTTGCAGTGAAGCCTTCAGAAAGTGCTTTCCAACGGAAAGAAAATTTACCCATTTCAAACTTTAGTAAGACTTCTGCTTCCAGTGATGCTGACACAAGGAATGAAGGAGCAAAATCCTTTCTAAATACATTGAAAAATCCAAAGAAACCAGA
ACCAGAAAAAGAGAAGAGCAAAGTTGATTCATCTGCAATTTTAGATTTTGATAGTCAAGGATCAAAAAGTTCAAAAAAGAAGGGCAAGAAAGGAAGGCAGATAGACCCTTCTTTGCTTGGATTTAAGGTTACAAGTAATCGCATCAATATGGGGGAGATTCAACACCCTGATGATTAAATTCCTCTAGTTGATTTGTATT
CTTAGAGTGGTTATCATAATCCAAGGTAAATATGCCATCATTGATTTGGTTTTTAAATGGGTCATATTCTTAAATTTTTTCTCTGTACAGGTATTTTTGGCATAGTTCCCCCCCTGATAGTTTTTTTTCCACTTATTATGAAATTGAATGATTCGAGTCTTTATCAAATGAATTTTCATTTTTTGCACCCAGTAATTGTT
TTCATGTCATTTGACAATGTTTTAGGGAAGACCGGAAATCTACCTTTTGTTTGAGGTGCAATTCTATTCTCTGTTGGTCTGTTCCTCTACTAACAAATTAAAGCCATTTATGCTTGTCACAGTACCTCTTGGTCCTTGCCATTGTGATCATGTTGTTATAGTAGGATTTGATTTATGTAAGTGGTTGAGTCGGTACTATT
TACAAAATATTATAATTAAGTAGAATCTCAACATGTGGCTTTTGTTAATTTTAGTACCTTTTGTTGTCAATTTATGGGACCTGTATATCTATGTTAGATGGAAAAATGTTTATCGTACAATTTTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTTGATGATCTATGTTAGATGGAAAAATGTTTATCGTACAATT
TTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTCGATGATAACTGTCCAGATTAAATGACATGAGTCACATTATGCAGTAATTTCATGAGCTTTGGCTTTAGATGTTGTGACAGAGGAGTATCTAGTGTCAAAGCTGTCCACCCGGTAATTTTACCTGTCACATCTGCATCTTGCCTAATTTGCATGTCTGGTTAG
TTACCTAAAGGGTTGAGATCATGGGCTAAGTAAAATAATGTGTCCACATGGGCCATACACTAGAAACCTATATGTTGCCCTGGCATACTTAATGGAATAAGTTAGATGCAGCAGTAGTTGCTGCAGGACCTATGACATTGAAACCATAGACTTCCCGAAAGAGGACCAAGTCAACATACATTGATCAATTCTGTATCCAA
AACTCCAGTTACTACATTCTATGTGTTATTTC


>TRINITY_DN121285_c1_g2_i4 from sample A2 / Example unspliced isoform:
GGATTGATTAGCGATAACATAAAATATTAATTAGCGAAAGATCGCGAGGAAGGGAAGGGTTCCAATTTCACATCTGTTGCTATCTCCATTTCCCTTCCCTCGACTTGATAAGTAGTAAGCGCATCACCTCCCTTGGTCGATTACTTTTAATTTCAGCGACATACAGCCCGATCTCTCTGCGCGCACGGACTTAAGACCCC
AGCCTTGGTATATCCCGCACTTGTTTCGCATTTGCCCTTGCTGCTGCCACTGCTTCAACCGCAAATCCCTGTCGTATCCTCGCCCTCCTTTACGAGCCATGGGTTTCATCCTTCCGAAACCCCATATGGTTTGAATTTGCATACCCCGTCCTGTTCTTTAGTAATTTCTTCCCGTACATTCGATTTTCAGGGCACAATCT
GCAAGAGTAGCGAACCTAACGAGGTAGTCGTTAAATACCCCTTGGATTTGGTTTTTGTTCGAGGCGTAAGAATGGCCGAAAGGAAGTCCGATCTTCCGCAGCATTCTGAGACGCAGGATCATCCGAATATCAAAGTGTTGTGCTTAGATGGAGACAGCTCGGGATTGTTGTCTGGTAACCCTGATGCGGAATTTAAAGAT
GTATCTGGGCCTGAAAATGTAATCCCTCTTTCTCCTCAATGGCTGCACTCAAGGGTTGGTGAGACCAAGGAACCGCGGACTCCAAACAGTTTTAGTCAGAGTGGAAGCACTGATGCAAATCTCAAAGAGCAATGGAGAGGAGAGGGTTCTCTTGACAAGAAAGATTGGCGGAGAACTGCTCCTGGTGAGAGCGACGGTGG
TCGGCGCTGGCGTGAAGAGGAAAGGGAAAATACTGCAGTTGCCAGAAGAGACCGTAGAAAAGAAGGGGACAGAGAAGTTGAAACTCGGAAGGTTGATCGTAGATCAGAGAATACTTCACTAAGAGAAGTTGCAGACAATAAGCATCCAAATTCGTCAGACCGTTGGCAAGATACTACTGCCCGTGATCCAGGCCACGAGG
CACGTCGTGACAGGAAATGGTCCACAGCATGGGGACCTGAAAACAATGAAAAAGATATACGTCGAGAGAAACGCAGTGAAGTGGACAAGGAGGATGGTGGTCTTGATAGACAACCTTCTTTTTCAAGCAGTCGTCTATTGGCTGCAGACCGTGAATCGGAAAAATCATCGACTGACAAGTGGAGACCATCCAGACTCCGG
GACAAACTTAAAACACAAAAGGATGGTGTTGGATTCCTTGCAGGTCGAGGCCGTGGAAATGGTTTGACAAATAGTCTTTCGATGAGAACTCCTATTGGTGCTTCTACTATTACAAATGCTTTTTGCTACCCACGGGCCAAACTTCTTGACATTTATAGGGATTGTGGATCTTTCTCACCTTTACGAGGTGTTGCCGATGG
ATTTGAGGAAGCTTCTGGCGTTACTTCTTCTGACCATTTTGAGCCATTGGCATTTGTCTCGCCTGAGCCAGAAGAGCAGGCTGTATTAGAAGACATTTCTAAGGGGAAAATTGCAGGCGGTGAAGCAATCCGCAGGACATTTAACAATACATCTGGCAGGATCAATATTGATGGCCTTGGAATTAGAGAGGAGATATTGT
CCAAGAGCAATAAGAAAAGGGATAGTGATGAATCTGTGAGCTGTGTGATGGATGATTCTCAATTAAAGGTGCATGAAGATGTGCTCCCTGATTTAGATGAGTCTGAACTTGTAGATTCAGTTTCCAAGGAGATCACAGCAGGCAGCAAAGATAAGACAATGTATTCAGTTCCCACATTATATGATTTCAATGATGAGATA
TTCATCCCAAAGGCTTCTGCTGAGGCAAGGAGGGCTGCCAAATCTTTTGAAGGAATATCTGAATATCCTTTCGTTGAAGAGATGATGGGTGAGGAGGTTTGTCATGAAAATGAAAATGTAAATTCATCAGGACTAGGTAATTCTTATGATAATCTATCCATGAAAGGAGAAGCATCAATGGCAGTTGGGGAGAGGAAGAG
TACAAAAGATATTGAAGTGACTTCTTCTGAGCTGCCTATCGGGCTACCATCTGATGCAAGTGTGCTTTTTGAAGACAAATCCTTTGAAGGGACTCCTGTAGAGTGTGATTTCGAGGATGGAAAATCAGGAAATAAGGATGGCATCAAGCTAGGAGAACAAGGTTTCCTGCTTGAGGAACTAAGTCTTTTTTATAAGGATC
CTCAAGGGGATATTCAGGGGCCTTTTCTAGGTATAGATATAATTCAATGGTTTGAACAAGGTTTTTTTGGTACAGATTTACTAGTGTGCCTTTCTGATGCTCCGGAGGGAACACCATTTCAGCCCCTTGGAGAGCTTATTCTACACATGAGACATAAAGCTCAATCATCTCATGGTACTGTCTTTGAGGATTCAGAAGAC
ACCAAAGGAATGAGTTCAAGAAGTGGTCATGATATTTCATCTAAAAAGGCTGGTATCATATATGAACATAATTCAATACTCAATCCTGAGCAAGAAAAGACATTGTCCGACTTTTCAGAGCAGGTCAATGCAGGATTAACTGGCAGAGGACTATCTTTTCCTGATATGCTCAAAGGTACTAAGAATAATACACTTGATCA
TCAAGAACAACACTTTTTGGAATCTGCTACAGAGAAAAATGCAGAAATTCTTGCAAGAATAATTCATCATGGAATACCACCGGGAGAATCTATTGGAAGTCTTGAGAGGCAGAGAATGTCTTCCTTACCTCGGTTTGATACAGATGATGGCCTTGTTCCTTGGCAATCTGAAGCAAATGCTTTCGGTTCAGTCTGGCCTC
AGCAGGAAGCGGATCAGTTCATTGATATAAGATCCGGTCTGCTGGATGCCACAGTTAGCAGTTCCATTTCTGGTAATATGCTTTCAATGATGTCAAGGATGCAATCCCTACCTAATAATCAGCGACAAGACTTATTTCATCAATCAAATGGTGCCGGCACTTCTCCTCCAATTGATAGGGCTTGGTCTGAAACATTTCAG
AGGGATGCATATTCCACTAACAAAATTTTCAATGTTGCATCTGATCCTCATCGTATGACACATCCTGAACAGAATTTGCAAAGAATAGAGCGAGCTAACCTTTTATTTCATCACCAGTTGGAGCAACAGAAGCAGAAACAGCAACAGCAGCAGCAACAACAACAACAAATGCTGATGCAACGGCAATTGCAGTCCGGAGC
TAAATTGCTGGATCACAGTACAATTTCAGGTATGGGTCATAACTTTGACTCTTCACTGTTGCAACACCACCAATTGCTACAACCACCTTTACAACCACGTCAGCAACTACCTCCATCTGCGCATGTTATGGACCCAATCTCAAGGCTTCAGCTGCAGCATCACCATCACCAACCCCAACACCATCTACAACTACAACCAC
ATCAGCAGCAAACCCAGCATAATATGCAGATGGAGCACCAACGGCAGTCTCATCAAACAGGCCTAGATCAGTTACTGTCTCATCAGATATATGATCCTAGCTATGCTCAGAATCAATTTCCTTCTGGTGGAAGTAGCATTGATCAGCTTATGTTAAGGCAAGAAGGTATTAGGCATGAATCCCAGCCACATCATCAGTCT
ATACGTAGGCAAAGCAATTCAATGGACCCAAGCCTTTCATTGGAACAACTTTTGCATCAGAGAAGACATGAGCTATCAGCTAACCAGTTTCCCTTGCCATCGCAACGGCAATTTATTGATGAAAGACGTGTAAGTGGAGTTTGGGCTGTTGATGAGTCTGGTGAGTTTGTACAGACGCAAGCACCTACTCTCCATCCCTT
TGATTTATACCAGCCGCATCAGCAGATACTGAATTCACAACCACAACACACACAATTATTAAATTTTCAATCTTCAAGCACCCTAGCATCTAATGCAGAAATAAAATATCCTTACCTATTTGAGCGGGCTGAGCATCATGACCATAGCAGTGAAGGTCGATCATTGCATGGTATGCATGATCCCAACCTGTTACCTTTTG
AAAGGCCTACTGATTTGTCTTCGCTGAACATGGACTTCTCACATTCTCTTAAACAAGCCCAGGAACGCAGGGTGCAGGAAAGGTATAATGTTACACGTTTGGCAGGACAGGATGAACAATTCCCATCAGAAGTTACCTACAATTCCCGATCTCATCCAATGGCCTTCCATGGTATACAATCTGAGGTTACAAATGGGATT
TGGGAGCAACCCTCCAGCACACATTCTACTGATCTTCATGTTCAGGGTCCTGGCTTTTTTATGCAGCAAGGACAACCTTTCGATTTTATTACTGAAAGCCAAGGTCAGAAGTTGCATCGAGTTGGGTTAAATTGGCAGTCCAATGAAGGTTCTCAAGAGCATGTTTCTTCTGAAGAATTGCACCGGAGATCTTTAATTTC
TACACACACTGCTGATTTAGAGACAAGACAATCTCATGGCGACTCGCTTCATCAAAAATTCCCTCTTCGTTCTGGTTCGTCATCTGTAATGCCTGgtaatgtgcttgctacttcttctgaacaaagccagtgtatgtggactgctgtgaactcaagagagtgtatttctggttgtacttatgattcttctgatttgattt
caagccagCTATTCAAAAATCGTAGAAATGAAGATCTTTCAGAGGTTCTAGAAGATTTGGCTGGGAATGGTGTTGGATCGACTTATATGGCCTGTAACTCAGCATCTGAAATGCAGATGGGGACTGGCTTATCTGTAGATAGTGTATATGATGGTATGCATTCAGAAGCATTTATTGAGGAGCCATTTTATTCATCAAAT
AACAATGAGCTGCAACTGTCTCTGCAAGTGTCTGATTCACTCGGGCAATCCTCACGAGATGACATTGAGAGTTTGGAATTCAAAGAAGCTAAAAAAAACAAACGGCGGAGTTCTAAAGCTAGAGGGACAGTAAAAATGACTGTTGTTTCTTCAGATGCGTCTTCAGTGTCAAACTTATCTACAGGAGCTCCAGAATTGAG
TCAAAATCAACAAACAATAAAGCATGACAGTTCCACTGTGCAAGAGTTGTCTTCATGGAGTACAGATTTTAATAGACGAGGGTTATCAGTGCATACTGGCATGGACACTAAAAAATCCCTACAGAAAACTGGGCAGTTGGTTATTGAAAGCAACAATGCAGATGGGAGAATGAGTCATTCCAGTGACAATGGTGATCAAA
TATCAACAGTTGAACGGGAAAATTCTAAATTTTCTGCAAAAGCTTTTGAATTGTCAAATTTGGCTTCTTCTCCCTTATCTACAGTACAGAGAAAGCTACTGAGTAACAGAAGAGCATCTTATGACGCTGGAGAAAGCATTGCAGAAAATGCTGGTGGCAATCTTGCAGTGAAGCCTTCAGAAAGTGCTTTCCAACGGAAA
GAAAATTTACCCATTTCAAACTTTAGTAAGACTTCTGCTTCCAGTGATGCTGACACAAGGAATGAAGGAGCAAAATCCTTTCTAAATACATTGAAAAATCCAAAGAAACCAGAACCAGAAAAAGAGAAGAGCAAAGTTGATTCATCTGCAATTTTAGATTTTGATAGTCAAGGATCAAAAAGTTCAAAAAAGAAGGGCAA
GAAAGGAAGGCAGATAGACCCTTCTTTGCTTGGATTTAAGGTTACAAGTAATCGCATCAATATGGGGGAGATTCAACACCCTGATGATTAAATTCCTCTAGTTGATTTGTATTCTTAGAGTGGTTATCATAATCCAAGGTAAATATGCCATCATTGATTTGGTTTTTAAATGGGTCATATTCTTAAATTTTTTCTCTGTA
CAGGTATTTTTGGCATAGTTCCCCCCCTGATAGTTTTTTTTCCACTTATTATGAAATTGAATGATTCGAGTCTTTATCAAATGAATTTTCATTTTTTGCACCCAGTAATTGTTTTCATGTCATTTGACAATGTTTTAGGGAAGACCGGAAATCTACCTTTTGTTTGAGGTGCAATTCTATTCTCTGTTGGTCTGTTCCTC
TACTAACAAATTAAAGCCATTTATGCTTGTCACAGTACCTCTTGGTCCTTGCCATTGTGATCATGTTGTTATAGTAGGATTTGATTTATGTAAGTGGTTGAGTCGGTACTATTTACAAAATATTATAATTAAGTAGAATCTCAACATGTGGCTTTTGTTAATTTTAGTACCTTTTGTTGTCAATTTATGGGACCTGTATA
TCTATGTTAGATGGAAAAATGTTTATCGTACAATTTTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTTGATGATCTATGTTAGATGGAAAAATGTTTATCGTACAATTTTTTTCTAGAGGCTGTGTTAGTGGTCAGTAAACTGGTCGATGATAACTGTCCAGATTAAATGACATGAGTCACATTATGCAGTAATT
TCATGAGCTTTGGCTTTAGATGTTGTGACAGAGGAGTATCTAGTGTCAAAGCTGTCCACCCGGTAATTTTACCTGTCACATCTGCATCTTGCCTAATTTGCATGTCTGGTTAGTTACCTAAAGGGTTGAGATCATGGGCTAAGTAAAATAATGTGTCCACATGGGCCATACACTAGAAACCTATATGTTGCCCTGGCATA
CTTAATGGAATAAGTTAGATGCAGCAGTAGTTGCTGCAGGACCTATGACATTGAAACCATAGACTTCCCGAAAGAGGACCAAGTCAACATACATTGATCAATTCTGTATCCAAAACTCCAGTTACTACATTCTATGTGTTATTTCCTATGTTGCATGGGTACCCGTACCGGGGGGTCGGGTACACGTACCCGTACCGGGT
ACTTGGGTACTTCAATGGTATGTTCCCAGCCGTACCCAAATTCAAACTCGCGTACCAGGTACTTCACTTATGAAAACAGGG