NA ----> NA

Intron 1 GTATGTCCCT ... TTGTGTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16	84	7.3	16
A2	11.94	59	6.9	8
A3	10.74	108	10.7	13
A4	9.62	47	11.6	5
A5	6.35	59	9.4	4
A6	12.3	1084	102.4	152
A7	11.13	965.6	102.6	121
A8	14.49	891	78.9	151
A9	13.08	923.9	97.1	139
A10	9.26	813	84.9	83

>TRINITY_DN37402_c0_g1_i4 from sample A7 / Example spliced isoform

GCCAGCTAGTTTTTCGTTGTTAGTAATTAATGACGCAGTATTCCAGTAGGCAGTTGTAGATTGACGTCGAGCCTTTTGGCACCTCGTACAGTAAATAGCTCGAAGTTTGGACTAAGACACAAGAGCGCTCTGGTTGCCAGCTTCCTTCGTTCAGTTAAGCTCACGGTTTCGCTACCCAACCTTTCAGCCGTAACCAATTT
TTGATTGATCAACAAATGGGCGAATCTTCCAGGGATTCAGAGGCTGCAGAATATACAGAAAAGTACAATAGATATGAGGCCGAGTATGTGCGTCGGGTGAAGGCCAAATATTTCTCTAAAAAATCATTATATGGGGGAACCATATTTGATAAAGAGACAACCGTGGATGATGAGATTATACGGGCAAGCAAGGATCCTCC
TATGAAACCCTTTATTGACGCATCTTGTTTAGGAGAGGAGCACAAGTCATCAACATCTGCAGGAGATCCTTCTGCTAATGCAGCAAATAGAAAATTACAGTCCAGAAAGAATGGGTAATGGATTCTGCCACCCCGGCAGTTCCTAATATAATGTCTAGGCAAATTAATAGGCAAAAAGTATAATCTAAACTTTTGGAATT
AGGTTTGGAAGTGAAGGTATGATTGTTGTTACGATGTCAACATCGTATTTTTGTCATATTTTTCATGTGTGATAGTCTGCTTAACTGAAATGCCAGGGAAACCCATATTCCATTTAGTATGATGTAACAGTTATAAACTGAGTAGAGCATCCATCGGCTCTGGTCCCCTTCAGATGGAATATGTATTTCTTTACAATTTT
AAAGTAGGAACAATGTGTATGATCAACTAAAATCGAATGGGATACTTCTCTTGTTTTGTTTATCCTGTTGAAAATAATTATATTATGTGATTGCCA

>TRINITY_DN37402_c0_g1_i5 from sample A7 / Example unspliced isoform:

ATGAGATTATACGGGCAAGCAAgtatgtccctcagatttctgatccctcctatttgatgatggctatttgactgggtagatgaagaaaagcataaatttacacatttcaaaactccaaaagtgttcacgttatcttctttttgttagcacatatgcaatttttttgacttatccatatgcttatgcatctctatttggtg
ttttgtgcttatgactaagtgaatttgtttttggcattgtgtttagGGATCCTCCTATGAAACCCTTTATTGACGCATCTTGTTTAGGAGAGGAGCACAAGTCATCAACATCTGCAGGAGATCCTTCTGCTAATGCAGCAAATAGAAAATTACAGTCCAGAAAGAATGGGTAATGGATTCTGCCACCCCGGCAGTTCCTA
ATATAATGTCTAGGCAAATTAATAGGCAAAAAGTATAATCTAAACTTTTGGAATTAGGTTTGGAAGTGAAGGTATGATTGTTGTTACGATGTCAACATCGTATTTTTGTCATATTTTTCATGTGTGATAGTCTGCTTAACTGAAATGCCAGGGAAACCCATATTCCATTTAGTATGATGTAACAGTTATAAACTGAGTAG
AGCATCCATCGGCTCTGGTCCCCTTCAGATGGAATATGTATTTCTTTACAATTTTAAAGTAGGAACAATGTGTATGATCAACTAAAATCGAATGGGATACTTCTCTTGTTTTGTTTATCCTGTTGAAAATAATTATATTATGTGATTGCCA

Intron 2 GTAAGAAACA ... ACGTGGAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.82	15	7	9.9
A2	10	27	3	11
A3	17.65	28	6	9.9
A4	44.44	20	16	12.2
A5	35	13	7	20.2
A6	14.77	300	52	107.2
A7	15.41	225	41	77.4
A8	14.77	254	44	69.6
A9	15.85	276	52	91.2
A10	18.8	190	44	41

>TRINITY_DN111005_c0_g1_i5 from sample A1 / Example spliced isoform

AATTAATGACGCAGTATTCCAGTAGGCAGTTGTAGATTGACGTCGAGCCTTTTGGCACCTCGTACAGTAAATAGCTCGAAGTTTGGACTAAGACACAAGAGCGCTCTGGTTGCCAGCTTCCTTCGTTCAGTTAAGCTCACGGTTTCGCTACCCAACCTTTCAGCCGTAACCAATTTTTGATTGATCAACAAATGGGCGAA
TCTTCCAGGGATTCAGAGGCTGCAGAATATACAGAAAAGTACAATAGATATGAGGCCGAGTATGTGCGTCGGGTGAAGGCCAAATATTTCTCTAAAAAATCATTATATGGGGGAACCATATTTGATAAAGAGACAACCGTGGATGATGAGATTATACGGGCAAGCAAGGATCCTCCTATGAAACCCTTTATTGACGCATC
TTGTTTAGGAGAGGAGCACAAGTCATCAACATCTGCAGGAGATCCTTCTGCTAATGCAGCAAATAGAAAATTACAGTCCAGAAAGAATGGGTAATGGATTCTGCCACCCCGGCAGTTCCTAATATAATGTCTAGGCAAATTAATAGGCAAAAAGTATAATCTAAACTTTTGGAATTAGGTTTGGAAGTGAAGGTATGATT
GTTGTTACGATGTCAACATCGTATTTTTGTCATATTTTTCATGTGTGATAGTCTGCTTAACTGAAATGCCAGGGAAACCCATATTCCATTTAGTATGATGTAACAGTTATAAACTGAGTAGAGCATCCATCGGCTCTGGTCCCCTTCAGATGGAATATGTATTTCTTTACAATTTTAAAGTAGGAACAATGTGTATGATC
AACTAAAATCGAATGGGATACTTCAAAAAAAA

>TRINITY_DN111005_c0_g1_i3 from sample A1 / Example unspliced isoform:

AATTAATGACGCAGTATTCCAGTAGGCAGTTGTAGATTGACGTCGAGCCTTTTGGCACCTCGTACAGTAAATAGCTCGAAGTTTGGACTAAGACACAAGAGCGCTCTGGTTGCCAGCTTCCTTCGTTCAGTTAAGCTCACGGTTTCGCTACCCAACCTTTCAGCCGTAACCAATTTTTGgtaagaaacatatagggtcta
ctcccctgtaataatatatgtttacatttatcattcttttacaagtactgtgctaacccttttctctttgatacaatatatatttgtatctgtgcgtacgtggaaagATTGATCAACAAATGGGCGAATCTTCCAGGGATTCAGAGGCTGCAGAATATACAGAAAAGTACAATAGATATGAGGCCGAGTATGTGCGTCGG
GTGAAGGCCAAATATTTCTCTAAAAAATCATTATATGGGGGAACCATATTTGATAAAGAGACAACCGTGGATGATGAGATTATACGGGCAAGCAAGGATCCTCCTATGAAACCCTTTATTGACGCATCTTGTTTAGGAGAGGAGCACAAGTCATCAACATCTGCAGGAGATCCTTCTGCTAATGCAGCAAATAGAAAATT
ACAGTCCAGAAAGAATGGGTAATGGATTCTGCCACCCCGGCAGTTCCTAATATAATGTCTAGGCAAATTAATAGGCAAAAAGTATAATCTAAACTTTTGGAATTAGGTTTGGAAGTGAAGGTATGATTGTTGTTACGATGTCAACATCGTATTTTTGTCATATTTTTCATGTGTGATAGTCTGCTTAACTGAAATGCCAG
GGAAACCCATATTCCATTTAGTATGATGTAACAGTTATAAACTGAGTAGAGCATCCATCGGCTCTGGTCCCCTTCAGATGGAATATGTATTTCTTTACAATTTTAAAGTAGGAACAATGTGTATGATCAACTAAAATCGAATGGGATACTTCAAAAAAAA

Intron 3 GTAAGAAACA ... AATCTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48	13	7	17
A2	61.29	6	3	16
A3	31.03	20	6	12
A4	51.61	15	16	16
A5	64	9	7	25
A6	44.46	158	52	201
A7	43	114	41	131
A8	38.77	139	44	132
A9	42.86	140	52	158
A10	40.06	98	44	87

>TRINITY_DN111005_c0_g1_i2 from sample A1 / Example spliced isoform

AATTAATGACGCAGTATTCCAGTAGGCAGTTGTAGATTGACGTCGAGCCTTTTGGCACCTCGTACAGTAAATAGCTCGAAGTTTGGACTAAGACACAAGAGCGCTCTGGTTGCCAGCTTCCTTCGTTCAGTTAAGCTCACGGTTTCGCTACCCAACCTTTCAGCCGTAACCAATTTTTGGGATTCAGAGGCTGCAGAATA
TACAGAAAAGTACAATAGATATGAGGCCGAGTATGTGCGTCGGGTGAAGGCCAAATATTTCTCTAAAAAATCATTATATGGGGGAACCATATTTGATAAAGAGACAACCGTGGATGATGAGATTATACGGGCAAGCAAGGATCCTCCTATGAAACCCTTTATTGACGCATCTTGTTTAGGAGAGGAGCACAAGTCATCAA
CATCTGCAGGAGATCCTTCTGCTAATGCAGCAAATAGAAAATTACAGTCCAGAAAGAATGGGTAATGGATTCTGCCACCCCGGCAGTTCCTAATATAATGTCTAGGCAAATTAATAGGCAAAAAGTATAATCTAAACTTTTGGAATTAGGTTTGGAAGTGAAGGTATGATTGTTGTTACGATGTCAACATCGTATTTTTG
TCATATTTTTCATGTGTGATAGTCTGCTTAACTGAAATGCCAGGGAAACCCATATTCCATTTAGTATGATGTAACAGTTATAAACTGAGTAGAGCATCCATCGGCTCTGGTCCCCTTCAGATGGAATATGTATTTCTTTACAATTTTAAAGTAGGAACAATGTGTATGATCAACTAAAATCGAATGGGATACTTCAAAAA
AAA

>TRINITY_DN111005_c0_g1_i3 from sample A1 / Example unspliced isoform:

AATTAATGACGCAGTATTCCAGTAGGCAGTTGTAGATTGACGTCGAGCCTTTTGGCACCTCGTACAGTAAATAGCTCGAAGTTTGGACTAAGACACAAGAGCGCTCTGGTTGCCAGCTTCCTTCGTTCAGTTAAGCTCACGGTTTCGCTACCCAACCTTTCAGCCGTAACCAATTTTTGgtaagaaacatatagggtcta
ctcccctgtaataatatatgtttacatttatcattcttttacaagtactgtgctaacccttttctctttgatacaatatatatttgtatctgtgcgtacgtggaaagattgatcaacaaatgggcgaatcttccagGGATTCAGAGGCTGCAGAATATACAGAAAAGTACAATAGATATGAGGCCGAGTATGTGCGTCGG
GTGAAGGCCAAATATTTCTCTAAAAAATCATTATATGGGGGAACCATATTTGATAAAGAGACAACCGTGGATGATGAGATTATACGGGCAAGCAAGGATCCTCCTATGAAACCCTTTATTGACGCATCTTGTTTAGGAGAGGAGCACAAGTCATCAACATCTGCAGGAGATCCTTCTGCTAATGCAGCAAATAGAAAATT
ACAGTCCAGAAAGAATGGGTAATGGATTCTGCCACCCCGGCAGTTCCTAATATAATGTCTAGGCAAATTAATAGGCAAAAAGTATAATCTAAACTTTTGGAATTAGGTTTGGAAGTGAAGGTATGATTGTTGTTACGATGTCAACATCGTATTTTTGTCATATTTTTCATGTGTGATAGTCTGCTTAACTGAAATGCCAG
GGAAACCCATATTCCATTTAGTATGATGTAACAGTTATAAACTGAGTAGAGCATCCATCGGCTCTGGTCCCCTTCAGATGGAATATGTATTTCTTTACAATTTTAAAGTAGGAACAATGTGTATGATCAACTAAAATCGAATGGGATACTTCAAAAAAAA