sp|P93447|EF1D_SPUBR ----> Elongation factor 1-delta

Intron 1 GTAAATCTAA ... TATTGGGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.6	92	25	9
A2	20.51	93	36	12
A3	15.74	99	19	18
A4	22.9	101	36	24
A5	24.56	43	13	15
A6	19.75	128	36	27
A7	21.91	155	59	28
A8	19.74	61	21	9
A9	20	118	36	23
A10	24.06	142	53	37

>TRINITY_DN97078_c1_g1_i2 from sample A5 / Example spliced isoform

AACTAACTCCTGTAGGCGGACGCGGCATGCAGAAAGTAGTCATATGGCGATTTAGGGTTTTGATTTGTTTCCTTTTAAGAGACCTCCAAACCCTCTTCGTATCCATAATCCTCTAATCGTCGGAAGCAGAATCAGAGGCACAGGGCGAGGGCACAAGAAGAGTAAATCCTTTCTGCACAGAAGAGAAGAAGAATGGCCGT
TACATTCTCCAATCTCAACACTGCATCAGGCGTGGAGCAGTTGGATCAGTTCCTGCTCGCAAGGAGTTACATTTCTGGGTATCAAGCATCAGCAGATGACATAAGAGTATACTGTGCACTTGGCAAGGCACCTGAATCCCAGTACATCAATGCCTCTCGTTGGTACAAGCACATCAGTGCCCTACTAGCCCCAAGTTTTT
CTGGGGAAGGCGTTGGTGTTCAAGTCCAAGGGGAGAGTCAGCCATCGACTGCATCAACCCCTGCCCCCGTCGAGGAGGAGGAGGACGAGGACGACGATGATGTGGATCTCTTCGGTGAAGAAACCGAAGAGGAAAAGAAGGCAGCTGAAGAACGCTCTGCAGCTGTAAAAGCCTCTATTAAAAAGAAACAGAGTGGAAAG
TCTTCAGTTCTTCTGGAAGTGAAGCCATGGGATGATGAAACAGATATGCAAAAGCTGGAGGAAACAGTACGCAGTCTCAAGATGGACGGCTTGCTGTGGGGAGCTTCAAAACTCATTCCGGTGGGATATGGAATTAAGAAGCTGCAGATAATGATGACCATCGAAGATGACCTTGTATCTGTCGACAATCTTATTGAGGA
TTATCTTACAGTTGAACCGGCCAATGAGTACATTCAGAGTTGTGATATTGTTGCATTCAATAAGATCTAATTGGGGAAAGCATGAAATTGACATCAAAATTGAATTGGTTGCTTCGCATGGTTAATAGCCTGAAGAGATTGTCTTCAGTGGAAGCTAGGAAACATGTAATTGGCAAGCCAGTCAATCGAGTTCCTTATGT
TTTTTTTAACAACTAGATCATTTTTGATGCAAAGTCTGGAATGAGAACGAGTACGAGTTTCTAAAAACTTGTTGAAGATTTTTTAATATAATTGTGGCCCGAGATTGCAGTCTTGTGCTTAGTTTTCAATATTTATGCAAGATTTTTTAATATAATTGTGATCCGATATTGCAGCATTGTGCTATAAAAGTTTTCAATAT
TTATGCATAATGGAAATCTTATATTTTCCTTTCGCTTCTGATTGATGCAAACTTGCTCCCACTATGCTTATGTTACCTATTGTCTTATAAAACTTGTTCCCAGTATGCTCATGTTACCTACTAGTCTTAGGGACTAGTACCCAATATTTACTTATTTTCAAGTTTCAACTCTACAATTTTTGTAATACCAGATAGTGGTT
TATTAG

>TRINITY_DN97078_c1_g1_i1 from sample A5 / Example unspliced isoform:

AACTAACTCCTGTAGGCGGACGCGGCATGCAGAAAGTAGTCATATGGCGATTTAGGGTTTTGATTTGTTTCCTTTTAAGAGACCTCCAAACCCTCTTCGTATCCATAATCCTCTAATCGTCGGAAGCAGAATCAGAGGCACAGGGCGAGGGCACAAGAAGAGTAAATCCTTTCTGCACAGAAGAGAAGAAGAATGGCCGT
TACATTCTCCAATCTCAACACTGCATCAGGCGTGGAGCAGTTGGATCAGTTCCTGCTCGCAAGGAGTTACATTTCTGGgtaaatctaaattctacctctggcccctttctttgatttccgttctggcctctttctttgatttctgttttattctgaaatgctagattggtttgttttattgggtagGTATCAAGCATCAG
CAGATGACATAAGAGTATACTGTGCACTTGGCAAGGCACCTGAATCCCAGTACATCAATGCCTCTCGTTGGTACAAGCACATCAGTGCCCTACTAGCCCCAAGTTTTTCTGGGGAAGGCGTTGGTGTTCAAGTCCAAGGGGAGAGTCAGCCATCGACTGCATCAACCCCTGCCCCCGTCGAGGAGGAGGAGGACGAGGAC
GACGATGATGTGGATCTCTTCGGTGAAGAAACCGAAGAGGAAAAGAAGGCAGCTGAAGAACGCTCTGCAGCTGTAAAAGCCTCTATTAAAAAGAAACAGAGTGAGTTTAGATTTTTGGTTAAATTCTTCAATATTTAGTCTTTTTTCAAGTGAATTTTGTGTTTGCACCAGAACAAGCATTAAGTATGCCTTTACCAGAC
CATGATTCATTTGGTTAGTTTATATTTATATTTATAATTTATAATATTTTACAAATGCTTCCTAAAAATATCCACGCTCTTCCCCATTATAGGGAGTATTGCAATGGAATGTCGGTGACTTGCAATACTCTTGAAACATGGATTCCTTTGTCTAAGAAGCTGTCAGTCGGGATTCTATTCTGTCAAATTTGCTTAGGGCT
TGCACTTGGTCCAACCACATTAAATGACTCTGAATTTGAA

Intron 2 GTAAGTTCTT ... TGTGATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	36.87	75.3	44	6
A2	34.34	65	34	15
A3	36.36	72.3	41.3	5
A4	29.69	45	19	2
A5	34.78	45	24	4
A6	32.57	282.3	136.3	72
A7	35.83	231	129	46.3
A8	32.41	196	94	33.3
A9	33.74	181.3	92.3	23.7
A10	34.14	118.3	61.3	20.3

>TRINITY_DN111597_c0_g1_i3 from sample A1 / Example spliced isoform

AATAATCAATATCCCTCCTCCCTCATTGAATGTTGGATGCATTAACATGTTGCACCTGTTTTGATGATCCCAACCACATCAAAAGTTCCACGAGGGGTGGCCAACCACTGTGTGACATGTTGGGCAAGGGGTGAAAAACTTGTCTCTGTTGGATTTAAACCTTAGTGGGATATGGAAATACTAATTTGTTTTTGCACGTA
ATAATTAAAATACTGTCTTCCATCATTGTTGGCTGTTGCACCTACCCATTGGCCCTAACCATGCGAATGTCCTCGAGAGGTGAAATTTGTCTATGTAGGTTTTGAACCTTGGTGGGTATGAAACACCTCTGCTTTACCAACTAACCAAAACCCATTGACAATTTTAATTGTTGTTTATTGCATTACTTCCAATAGTCAAA
ATTCATGCGCACTCATGGTTGGTTGGATCCATTAACAGGTGGAAAGTCTTCAGTTCTTCTGGAAGTGAAGCCATGGGATGATGAAACAGATATGCAAAAGCTGGAGGAAACAGTACGCAGTCTCAAGATGGACGGCTTGCTGTGGGGAGCTTCAAAACTCATTCCGGTGGGATATGGAATTAAGAAGCTGCAGATAATGA
TGACCATCGAAGATGACCTTGTATCTGTCGACAATCTTATTGAGGATTATCTTACAGTTGAACCGGCCAATGAGTACATTCAGAGTTGTGATATTGTTGCATTCAATAAGATCTAATTGGGGAAAGCATGAAATTGACATCAAAATTGAATTGGTTGCTTCGCATGGTTAATAGCCTGAAGAGATTGTCTTCAGTGGAAG
CTAGTAAACATGTAATTGGCAAGCCAGTCAATCGAGTTCCTTATGTTTTTTTTAACAACTAGATCATTTTTGATGCAAAGTCTGGAATGAGAACGAGTACGAGTTTCTAAAAACTTGTTGAAGATTTTTTAATATAATTGTGGCCCGAGATTGCAGTCTTGTGCTTAGTTTTCAATATTTATGCAAGATTTTTTAATATA
ATTGTGATCCGATATTGCAGCATTGTGCTATAAAAGTTTTCAATATTTATGCATAATGGAAATCTTATATGTTCCTTTCGCTTCTGATTGATGCAAACTTGCTCC

>TRINITY_DN111597_c0_g1_i1 from sample A1 / Example unspliced isoform:

CAAGGTTTCATGGATTTTGGACCATTGAGTTAAAACTAACTCCTGTAGGCGGACGCGGCATGCAGAAAGTAGTCATATGGCGATTTAGGGTTTTGATTTGTTTCCTTTTAAGAGACCTCCAAACCCTCTTCGTATCCATAATCCTCTAATCGTCGGAAGCAGAATCAGAGGCACAGGGCGAGGGCACAAGAAGAGTAAAT
CCTTTCTGCACAGAAGAGAAGAAGAATGGCCGTTACATTCTCCAATCTCAACACTGCATCAGGCGTGGAGCAGTTGGATCAGTTCCTGCTCGCAAGGAGTTACATTTCTGGGTATCAAGCATCAGCAGATGACATAAGAGTATACTGTGCACTTGGCAAGGCACCTGAATCCCAGTACATCAATGCCTCTCGTTGGTACA
AGCACATCAGTGCCCTACTAGCCCCAAGTTTTTCTGGGGAAGGCGTTGGTGTTCAAGTCCAAGGGGAGAGTCAGCCATCGACTGCATCAACCCCTGCCCCCGTCGAGGAGGAGGAGGACGAGGACGACGATGATGTGGATCTCTTCGGTGAAGAAACCGAAGAGGAAAAGAAGGCAGCTGAAGAACGCTCTGCAGCTGTA
AAAGCCTCTATTAAAAAGAAACAGAGTGGAAAGTCTTCAGTTCTTCTGGAAGTGAAGCCATGGGATGATGAAACAGATATGCAAAAGCTGGAGGAAACAGTACGCAGTCTCAAGATGGACGGCTTGCTGTGGGGAGCTTCAAAACTCATTCCGGTGGGATATGGAATTAAGAAGCTGCAGATAATGATGACCATCGAAGA
TGACCTTGTATCTGTCGACAATCTTATTGAGGATTATCTTACAGTTGAACCGGCCAATGAGTACATTCAGAGTTGTGATATTGTTGCATTCAATAAGATCTgtaagttcttatttaagtactttagccatttgttttgttctactgtatatgcttgctccttgttctaatttgaggttcttttgttatgatgtgatctgt
gtgattcagAATTGGGGAAAGCATGAAATTGACATCAAAATTGAATTGGTTGCTTCGCATGGTTAATAGCCTGAAGAGATTGTCTTCAGTGGAAGCTAGTAAACATGTAATTGGCAAGCCAGTCAATCGAGTTCCTTATGTTTTTTTTAACAACTAGATCATTTTTGATGCAAAGTCTGGAATGAGAACGAGTACGAGTT
TCTAAAAACTTGTTGAAGATTTTTTAATATAATTGTGGCCCGAGATTGCAGTCTTGTGCTTAGTTTTCAATATTTATGCAAGATTTTTTAATATAATTGTGATCCGATATTGCAGCATTGTGCTATAAAAGTTTTCAATATTTATGCATAATGGAAATCTTATATGTTCCTTTCGCTTCTGATTGATGCAAACTTGCTCC

Intron 3 GTAAATCTAA ... TATTGGGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.33	92	20.2	10.6
A2	15.75	93	29.6	17.4
A3	16.1	99	15.2	19
A4	19.84	101	30.8	25
A5	29.69	43	10.4	18.2
A6	16.88	128	28.8	26
A7	16.67	155	47.2	31
A8	16.44	61	16.8	12
A9	16.78	118	28.8	23.8
A10	21.11	142	42.4	38

>TRINITY_DN111597_c0_g1_i1 from sample A1 / Example spliced isoform

CAAGGTTTCATGGATTTTGGACCATTGAGTTAAAACTAACTCCTGTAGGCGGACGCGGCATGCAGAAAGTAGTCATATGGCGATTTAGGGTTTTGATTTGTTTCCTTTTAAGAGACCTCCAAACCCTCTTCGTATCCATAATCCTCTAATCGTCGGAAGCAGAATCAGAGGCACAGGGCGAGGGCACAAGAAGAGTAAAT
CCTTTCTGCACAGAAGAGAAGAAGAATGGCCGTTACATTCTCCAATCTCAACACTGCATCAGGCGTGGAGCAGTTGGATCAGTTCCTGCTCGCAAGGAGTTACATTTCTGGGTATCAAGCATCAGCAGATGACATAAGAGTATACTGTGCACTTGGCAAGGCACCTGAATCCCAGTACATCAATGCCTCTCGTTGGTACA
AGCACATCAGTGCCCTACTAGCCCCAAGTTTTTCTGGGGAAGGCGTTGGTGTTCAAGTCCAAGGGGAGAGTCAGCCATCGACTGCATCAACCCCTGCCCCCGTCGAGGAGGAGGAGGACGAGGACGACGATGATGTGGATCTCTTCGGTGAAGAAACCGAAGAGGAAAAGAAGGCAGCTGAAGAACGCTCTGCAGCTGTA
AAAGCCTCTATTAAAAAGAAACAGAGTGGAAAGTCTTCAGTTCTTCTGGAAGTGAAGCCATGGGATGATGAAACAGATATGCAAAAGCTGGAGGAAACAGTACGCAGTCTCAAGATGGACGGCTTGCTGTGGGGAGCTTCAAAACTCATTCCGGTGGGATATGGAATTAAGAAGCTGCAGATAATGATGACCATCGAAGA
TGACCTTGTATCTGTCGACAATCTTATTGAGGATTATCTTACAGTTGAACCGGCCAATGAGTACATTCAGAGTTGTGATATTGTTGCATTCAATAAGATCTGTAAGTTCTTATTTAAGTACTTTAGCCATTTGTTTTGTTCTACTGTATATGCTTGCTCCTTGTTCTAATTTGAGGTTCTTTTGTTATGATGTGATCTGT
GTGATTCAGAATTGGGGAAAGCATGAAATTGACATCAAAATTGAATTGGTTGCTTCGCATGGTTAATAGCCTGAAGAGATTGTCTTCAGTGGAAGCTAGTAAACATGTAATTGGCAAGCCAGTCAATCGAGTTCCTTATGTTTTTTTTAACAACTAGATCATTTTTGATGCAAAGTCTGGAATGAGAACGAGTACGAGTT
TCTAAAAACTTGTTGAAGATTTTTTAATATAATTGTGGCCCGAGATTGCAGTCTTGTGCTTAGTTTTCAATATTTATGCAAGATTTTTTAATATAATTGTGATCCGATATTGCAGCATTGTGCTATAAAAGTTTTCAATATTTATGCATAATGGAAATCTTATATGTTCCTTTCGCTTCTGATTGATGCAAACTTGCTCC

>TRINITY_DN111597_c0_g1_i2 from sample A1 / Example unspliced isoform:

CAAGGTTTCATGGATTTTGGACCATTGAGTTAAAACTAACTCCTGTAGGCGGACGCGGCATGCAGAAAGTAGTCATATGGCGATTTAGGGTTTTGATTTGTTTCCTTTTAAGAGACCTCCAAACCCTCTTCGTATCCATAATCCTCTAATCGTCGGAAGCAGAATCAGAGGCACAGGGCGAGGGCACAAGAAGAGTAAAT
CCTTTCTGCACAGAAGAGAAGAAGAATGGCCGTTACATTCTCCAATCTCAACACTGCATCAGGCGTGGAGCAGTTGGATCAGTTCCTGCTCGCAAGGAGTTACATTTCTGGgtaaatctaaattctacctctggcccctttctttgatttccgttctggcctctttctttgatttctgttttattgggtagGTATCAAGC
ATCAGCAGATGACATAAGAGTATACTGTGCACTTGGCAAGGCACCTGAATCCCAGTACATCAATGCCTCTCGTTGGTACAAGCACATCAGTGCCCTACTAGCCCCAAGTTTTTCTGGGGAAGGCGTTGGTGTTCAAGTCCAAGGGGAGAGTCAGCCATCGACTGCATCAACCCCTGCCCCCGTCGAGGAGGAGGAGGACG
AGGACGACGATGATGTGGATCTCTTCGGTGAAGAAACCGAAGAGGAAAAGAAGGCAGCTGAAGAACGCTCTGCAGCTGTAAAAGCCTCTATTAAAAAGAAACAGAGTGGAAAGTCTTCAGTTCTTCTGGAAGTGAAGCCATGGGATGATGAAACAGATATGCAAAAGCTGGAGGAAACAGTACGCAGTCTCAAGATGGAC
GGCTTGCTGTGGGGAGCTTCAAAACTCATTCCGGTGGGATATGGAATTAAGAAGCTGCAGATAATGATGACCATCGAAGATGACCTTGTATCTGTCGACAATCTTATTGAGGATTATCTTACAGTTGAACCGGCCAATGAGTACATTCAGAGTTGTGATATTGTTGCATTCAATAAGATCTAATTGGGGAAAGCATGAAA
TTGACATCAAAATTGAATTGGTTGCTTCGCATGGTTAATAGCCTGAAGAGATTGTCTTCAGTGGAAGCTAGTAAACATGTAATTGGCAAGCCAGTCAATCGAGTTCCTTATGTTTTTTTTAACAACTAGATCATTTTTGATGCAAAGTCTGGAATGAGAACGAGTACGAGTTTCTAAAAACTTGTTGAAGATTTTTTAAT
ATAATTGTGGCCCGAGATTGCAGTCTTGTGCTTAGTTTTCAATATTTATGCAAGATTTTTTAATATAATTGTGATCCGATATTGCAGCATTGTGCTATAAAAGTTTTCAATATTTATGCATAATGGAAATCTTATATGTTCCTTTCGCTTCTGATTGATGCAAACTTGCTCC

Intron 4 GTGAGTTTAT ... TTTAATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.38	77	0	14
A2	19.19	80	0	19
A3	14	43	1	7
A4	21.18	67	0	18
A5	30.23	30	0	13
A6	16.86	143	0	29
A7	21.15	123	0	33
A8	16.16	83	0	16
A9	20.92	121	0	32
A10	26.9	106	0	39

>TRINITY_DN32042_c0_g1_i1 from sample A8 / Example spliced isoform

ACTAACTCCTGTAGGCGGACGCGGCATGCAGAAAGTAGTCATATGGCGATTTAGGGTTTTGATTTGTTTCCTTTTAAGAGACCTCCAAACCCTCTTCGTATCCATAATCCTCTAATCGTCGGAAGCAGAATCAGAGGCACAGGGCGAGGGCACAAGAAGAGTAAATCCTTTCTGCACAGAAGAGAAGAAGAATGGCCGTT
ACATTCTCCAATCTCAACACTGCATCAGGCGTGGAGCAGTTGGATCAGTTCCTGCTCGCAAGGAGTTACATTTCTGGGTATCAAGCATCAGCAGATGACATAAGAGTATACTGTGCACTTGGCAAGGCACCTGAATCCCAGTACATCAATGCCTCTCGTTGGTACAAGCACATCAGTGCCCTACTAGCCCCAAGTTTTTC
TGGGGAAGGCGTTGGTGTTCAAGTCCAAGGGGAGAGTCAGCCATCGACTGCATCAACCCCTGCCCCCGTCGAGGAGGAGGAGGACGAGGACGACGATGATGTGGATCTCTTCGGTGAAGAAACCGAAGAGGAAAAGAAGGCAGCTGAAGAACGCTCTGCAGCTGTAAAAGCCTCTATTAAAAAGAAACAGAGTGGAAAGT
CTTCAGTTCTTCTGGAAGTGAAGCCATGGGATGATGAAACAGATATGCAAAAGCTGGAGGAAACAGTACGCAGTCTCAAGATGGACGGCTTGCTGTGGGGAGCTTCAAAACTCATTCCGGTGGGATATGGAATTAAGAAGCTGCAGATAATGATGACCATCGAAGATGACCTTGTATCTGTCGACAATCTTATTGAGGAT
TATCTTACAGTTGAACCGGCCAATGAGTACATTCAGAGTTGTGATATTGTTGCATTCAATAAGATCTAATTGGGGAAAGCATGAAATTGACATCAAAATTGAATTGGTTGCTTCGCATGGTTAATAGCCTGAAGAGATTGTCTTCAGTGGAAGCTAGGAAACATGTAATTGGCAAGCCAGTCAATCGAGTTCCTTATGTT
TTTTTTAACAACTAGATCATTTTTGATGCAAAGTCTGGAATGAGAACGAGTACGAGTTTCTAAAAACTTGTTGAAGATTTTTTAATATAATTGTGGCCCGAGATTGCAGTCTTGTGCTTAGTTTTCAATATTTATGCAAGATTTTTTAATATAATTGTGATCCGATATTGCAGCATTGTGCTATAAAAGTTTTCAATATT
TATGCATAATGGAAATCTTATATTTTCCTTTCGCTTCTGATTGATGCAAACTTGCC

>TRINITY_DN32042_c0_g1_i2 from sample A8 / Example unspliced isoform:

AGGGGAGAGTCAGCCATCGACTgtgagtttatgtttgatctcgcaggatttttttttaaattttttttctttatttgtttgctggttttgagattgcaataggatatttagtaatagtgatggcgagggtatgtctgtttaatgtagGCATCAACCCCTGCCCCCGTCGAGGAGGAGGAGGACGAGGACGACGATGATGT
GGATCTCTTCGGTGAAGAAACCGAAGAGGAAAAGAAGGCAGCTGAAGAACGCTCTGCAGCTGTAAAAGCCTCTATTAAAAAGAAACAGAGTGGAAAGTCTTCAGTTCTTCTGGAAGTGAAGCCATGGGATGATGAAACAGATATGCAAAAGCTGGAGGAAACAGTACGCAGTCTCAAGATGGACGGCTTGCTGTGGGGAG
CTTCAAAACTCATTCCGGTGGGATATGGAATTAAGAAGCTGCAGATAATGATGACCATCGAAGATGACCTTGTATCTGTCGACAATCTTATTGAGGATTATCTTACAGTTGAACCGGCCAATGAGTACATTCAGAGTTGTGATATTGTTGCATTCAATAAGATCTAATTGGGGAAAGCATGAAATTGACATCAAAATTGA
ATTGGTTGCTTCGCATGGTTAATAGCCTGAAGAGATTGTCTTCAGTGGAAGCTAGGAAACATGTAATTGGCAAGCCAGTCAATCGAGTTCCTTATGTTTTTTTTAACAACTAGATCATTTTTGATGCAAAGTCTGGAATGAGAACGAGTACGAGTTTCTAAAAACTTGTTGAAGATTTTTTAATATAATTGTGGCCCGAG
ATTGCAGTCTTGTGCTTAGTTTTCAATATTTATGCAAGATTTTTTAATATAATTGTGATCCGATATTGCAGCATTGTGCTATAAAAGTTTTCAATATTTATGCATAATGGAAATCTTATATTTTCCTTTCGCTTCTGATTGATGCAAACTTGCC

Intron 5 GTACGCCTGT ... GAATCGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	11.41	46.7	6	9
A2	10.16	71	8	1
A3	10.45	69.7	8	0
A4	3.4	57	2	0
A5	8.51	43.3	4	0
A6	8.28	101	9	0
A7	4.65	124.7	6	0
A8	7.9	47	4	2
A9	4.9	117.3	6	0
A10	1.66	118.7	2	0

>TRINITY_DN111597_c0_g1_i2 from sample A1 / Example spliced isoform

CAAGGTTTCATGGATTTTGGACCATTGAGTTAAAACTAACTCCTGTAGGCGGACGCGGCATGCAGAAAGTAGTCATATGGCGATTTAGGGTTTTGATTTGTTTCCTTTTAAGAGACCTCCAAACCCTCTTCGTATCCATAATCCTCTAATCGTCGGAAGCAGAATCAGAGGCACAGGGCGAGGGCACAAGAAGAGTAAAT
CCTTTCTGCACAGAAGAGAAGAAGAATGGCCGTTACATTCTCCAATCTCAACACTGCATCAGGCGTGGAGCAGTTGGATCAGTTCCTGCTCGCAAGGAGTTACATTTCTGGGTAAATCTAAATTCTACCTCTGGCCCCTTTCTTTGATTTCCGTTCTGGCCTCTTTCTTTGATTTCTGTTTTATTGGGTAGGTATCAAGC
ATCAGCAGATGACATAAGAGTATACTGTGCACTTGGCAAGGCACCTGAATCCCAGTACATCAATGCCTCTCGTTGGTACAAGCACATCAGTGCCCTACTAGCCCCAAGTTTTTCTGGGGAAGGCGTTGGTGTTCAAGTCCAAGGGGAGAGTCAGCCATCGACTGCATCAACCCCTGCCCCCGTCGAGGAGGAGGAGGACG
AGGACGACGATGATGTGGATCTCTTCGGTGAAGAAACCGAAGAGGAAAAGAAGGCAGCTGAAGAACGCTCTGCAGCTGTAAAAGCCTCTATTAAAAAGAAACAGAGTGGAAAGTCTTCAGTTCTTCTGGAAGTGAAGCCATGGGATGATGAAACAGATATGCAAAAGCTGGAGGAAACAGTACGCAGTCTCAAGATGGAC
GGCTTGCTGTGGGGAGCTTCAAAACTCATTCCGGTGGGATATGGAATTAAGAAGCTGCAGATAATGATGACCATCGAAGATGACCTTGTATCTGTCGACAATCTTATTGAGGATTATCTTACAGTTGAACCGGCCAATGAGTACATTCAGAGTTGTGATATTGTTGCATTCAATAAGATCTAATTGGGGAAAGCATGAAA
TTGACATCAAAATTGAATTGGTTGCTTCGCATGGTTAATAGCCTGAAGAGATTGTCTTCAGTGGAAGCTAGTAAACATGTAATTGGCAAGCCAGTCAATCGAGTTCCTTATGTTTTTTTTAACAACTAGATCATTTTTGATGCAAAGTCTGGAATGAGAACGAGTACGAGTTTCTAAAAACTTGTTGAAGATTTTTTAAT
ATAATTGTGGCCCGAGATTGCAGTCTTGTGCTTAGTTTTCAATATTTATGCAAGATTTTTTAATATAATTGTGATCCGATATTGCAGCATTGTGCTATAAAAGTTTTCAATATTTATGCATAATGGAAATCTTATATGTTCCTTTCGCTTCTGATTGATGCAAACTTGCTCC

>TRINITY_DN111597_c0_g1_i4 from sample A1 / Example unspliced isoform:

CAAGGTTTCATGGATTTTGGACCATTGAGTTAAAACTAACTCCTGTAGGCGGACGCGGCATGCAGAAAGTAGTCATATGGCGATTTAGGGTTTTGATTTGTTTCCTTTTAAGAGACCTCCAAACCCTCTTCGTATCCATAATCCTCTAATCGTCGGAAGCAGAATCAGAGGCACAGGGCGAGGGCACAAGAAGAGTAAAT
CCTTTCTGCACAGAAGAGAAGAAGgtacgcctgtgttgagtattcctagcttgatttcattttctttttaatctattgcccagggttataataaggttgaatcgacagAATGGCCGTTACATTCTCCAATCTCAACACTGCATCAGGCGTGGAGCAGTTGGATCAGTTCCTGCTCGCAAGGAGTTACATTTCTGGGTA

Intron 6 GTACCCCTTT ... CCACTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	8.54	75	0	7
A2	7.58	61	0	5
A3	11.29	55	1	7
A4	5.97	63	0	4
A5	12.25	21.5	0	3
A6	16.93	132.5	0	27
A7	18.87	129	0	30
A8	15.12	73	0	13
A9	15.15	112	0	20
A10	11.29	110	0	14

>TRINITY_DN101501_c0_g1_i4 from sample A3 / Example spliced isoform

ATTCTACCTCTGGCCCCTTTCTTTGATTTCCGTTCTGGCCTCTTTCTTTGATTTCTGTTTTATTGGGTAGGTATCAAGCATCAGCAGATGACATAAGAGTATACTGTGCACTTGGCAAGGCACCTGAATCCCAGTACATCAATGCCTCTCGTTGGTACAAGCACATCAGTGCCCTACTAGCCCCAAGTTTTTCTGGGGAA
GGCGTTGGTGTTCAAGTCCAAGGGGAGAGTCAGCCATCGACTGCATCAACCCCTGCCCCCGTCGAGGAGGAGGAGGACGAGGACGACGATGATGTGGATCTCTTCGGTGAAGAAACCGAAGAGGAAAAGAAGGCAGCTGAAGAACGCTCTGCAGCTGTAAAAGCCTCTATTAAAAAGAAACAGA

>TRINITY_DN101501_c0_g1_i5 from sample A3 / Example unspliced isoform:

ATCAGTGCCCTACTAGCCCCAAGgtacccctttaactagtactgtctgtttatacaaattttgttccttttataaatggattggctaaagtagttttaccactggcagTTTTTCTGGGGAAGGCGTTGGTGTTCAAGTCCAAGGGGAGAGTCAGCCATCGACTGCATCAACCCCTGCCCCCGTCGAGGAGGAGGAGGACG
AGGACGACGATGATGTGGATCTCTTCGGTGAAGAAACCGAAGAGGAAAAGAAGGCAGCTGAAGAACGCTCTGCAGCTGTAAAAGCCTCTATTAAAAAGAAACAGAGTGAGTTTAGATTTTTGGTTAAATTCTTCAATATTTAGTCTTTTTTCAAGTGAATTTTGTGTTTGCACCAGAACAAGCATTAAGTATGCCTTTAC
CAGACCATGATTCATTTGGTTAGTTTATATTTATATTTATAATTTATAATATTTTACAAATGCTTCCTAAAAA