sp|Q9SJ61|CDPKP_ARATH ----> Calcium-dependent protein kinase 25

Intron 1 GTGAGTGGGT ... ATTGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.62	2323.1	404.4	131.4
A2	14.85	2081.1	370.6	160
A3	14.65	2519.6	440.7	179.6
A4	17.05	1622	340.7	117.3
A5	15.14	1341.7	242.7	68.9
A6	13.19	25871	4000.6	1465.1
A7	14.27	20577.4	3488.3	1113.7
A8	12.84	25071.9	3764.6	1617.4
A9	13.44	21947.6	3476.3	1203.4
A10	14.46	14521.9	2511.7	785.1

>TRINITY_DN125403_c3_g4_i2 from sample A1 / Example spliced isoform

TTGTATGTTCTTCTCTGTGGGAATAAACCTTTTGATGTGGAGACTGAAGACCCCAAGAGCATGTACCAGAAAATACAGAATACAGAACCAGATCTCAGTACTGGTCAATGGAGCTCCATATCCGAATCTGCAAAGAAACTCGTTCTTGGAATGTTGGAGAAGGGCTTCAACAACCGACTTTCTTGCGATCAAATTCTGAG
TCATCCGTGGATCTTGAGCAATGCAGCAGAGATGGACATTGCTTCTGTTTCCACCGTCAACGCAGAGATGGGTTCTACCTCCGGGGACGATGCAACGGATTGAGTGACAATTCATATAAAAATAATATGATAATAAAGAATGTAATAGAGGACCGCTTATATCATATATTTTGTGGTTGAATCATTTTGAACGACGAACT
TGCTGGAGGCTTCGAATTAGTGTTTTATTGTTTTTTATTAATATACATGTTTCACTTTCATTTCTCCTAATTTCATCCCTGGTTTG

>TRINITY_DN125403_c3_g4_i3 from sample A1 / Example unspliced isoform:

TTTCTTGCGATCAAATTCTGAgtgagtgggttatgatttgctttcaattttgcaaaattagttttgtgacttggaattcggaagttctttgtgggattggaataaattagtgagtgaaggtaatgaaatattattgatgcagGTCATCCGTGGATCTTGAGCAATGCAGCAGAGATGGACATTGCTTCTGTTTCCACCGT
CAACGCAGAGATGGGTTCTACCTCCGGGGACGATGCAACGGATTGAGTGACAATTCATATAAAAATAATATGATAATAAAGAATGTAATAGAGGACCGCTTATATCATATATTTTGTGGTTGAATCATTTTGAACGACGAACTTGCTGGAGGCTTCGAATTAGTGTTTTATTGTTTTTTATTAATATACATGTTTCACTT
TCATTTCTCCTAATTTCATCCCTGGTTTG

Intron 2 GTAAGTTGGT ... TCTTGAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.38	3020.5	749	522
A2	16.61	2428	603	364
A3	17.66	2973.5	765	510
A4	18.12	2237	557	433
A5	18.02	1321.5	362	219
A6	17.05	19084	4418	3427
A7	17.27	17181.5	4237	2934
A8	17.85	16308	3600	3489
A9	17.24	17789	4329	3083
A10	16.84	14180.5	3555	2186.5

>TRINITY_DN46042_c0_g2_i17 from sample A8 / Example spliced isoform

GAATAAACCTTTTGATGTGGAGATCTCCAGCGCTTTCTTGAAAGAAATGGCATCCCTTCAGAGAGGGAAGCCGCCAGAATAATGAAATGTATCGTGGAAGCTATTCGGTACTGCCATTCGCAGAGAATTATTCATAGAGACGTAAAGCCATCCAGCATCTTTATATCAGAGACTGAAAACTCCGGCTCATCATGGGTTGT
TAAACTCGGAAACTTCGATCGATCAATTCTGGTTCCACCATTTAATGCGCCTGTAATCAAAGAAAACGTGGGGACATTGGGATTTTCTGCTCCAGAGATGCTGAGCGGCAATGGCTACTCGTACAAGGTTGACATATGGAGCGCCGGAGTTGTGTTGTATGTTCTTCTCTGTGGGAATAAACCTTTTGATGTGGAGACTG
AAGACCCCAAGAGCATGTACCAGAAAATACAGAATACAGAAACAGATCTCAGTACTGGTCAATGGACCTCCATATCCCAATCTGCAAAGGAACTCGTTCTTGGAATGTTGGAGAAGGGCTTCAACAACCGACTTTCTTGCGATCAAATTCTGAGTCATCCGTGGATCTTGAGCAATGCAGCAGAGATGGACATTGCTTCT
GTTTCCACCGTCAACGCAGAGATGGGTTCTACCTCCGGGGACGATGCAACGGATTGAGTGACAATTCATATAAAAATAATATGATAATAAAGAATGTAATAGAGGACCGCTTATATCATATATTTTGTGGTTGAATCATTTTGAACGACGAACTTGCTGGAGGCTTCGAATTAGTGTTTTATTGTTTTTTATTAATATAC
ATGTTTCACTTTCATTTCTCCTAATTTCAAAAAATAAATAAAAAATAAAAAAATTAATAAAATAAAAA

>TRINITY_DN46042_c0_g2_i24 from sample A8 / Example unspliced isoform:

GAATAAACCTTTTGATGTGGAGATCTCCAGCGCTTTCTTGAAAGAAATGGCATCCCTTCAGAGAGGGAAGCCGCCAGAATAATGAAATGTATCGTGGAAGCTATTCGGTACTGCCATTCGCAGAGAATTATTCATAGAGACGTAAAGCCATCCAGCATCTTTATATCAGAGACTGAAAACTCCGGCTCATCATGGGTTGT
TAAACTCGGAAACTTCGATCGATCAATTCTGGTTCCACCATgtaagttggtaaaatatttttcaagattaggttataactatattttttgtattttaatttttcggccatctttttggcatgtgatcgatcttaatataaagtggagatttcttccattaagttcaaaacagtacggccaattagccaaatcgaatcgga
cttaatttataatatgttgatagtgatggattaatatttattcttgaacagTTAATGCGCCTGTAATCAAAGAAAACGTGGGGACATTGGGATTTTCTGCTCCAGAGATGCTGAGCGGCAATGGCTACTCGTACAAGGTTGACATATGGAGCGCCGGAGTTGTGTTGTATGTTCTTCTCTGTGGGAATAAACCTTTTGAT
GTGGAGACTGAAGACCCCAAGAGCATGTACCAGAAAATACAGAATACAGAAACAGATCTCAGTACTGGTCAATGGACCTCCATATCCCAATCTGCAAAGGAACTCGTTCTTGGAATGTTGGAGAAGGGCTTCAACAACCGACTTTCTTGCGATCAAATTCTGAGTCATCCGTGGATCTTGAGCAATGCAGCAGAGATGGA
CATTGCTTCTGTTTCCACCGTCAACGCAGAGATGGGTTCTACCTCCGGGGACGATGCAACGGATTGAGTGACAATTCATATAAAAATAATATGATAATAAAGAATGTAATAGAGGACCGCTTATATCATATATTTTGTGGTTGAATCATTTTGAACGACGAACTTGCTGGAGGCTTCGAATTAGTGTTTTATTGTTTTTT
ATTAATATACATGTTTCACTTTCATTTCTCCTAATTTCAAAAAATAAATAAAAAATAAAAAAATTAATAAAATAAAAA

Intron 3 GTGAGTGGGT ... ATTGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	4.99	2258.5	134.8	106.9
A2	4.5	1993.3	106	107.6
A3	4.73	2432.1	138.9	102.3
A4	5.42	1557.5	104.5	72.4
A5	4.86	1282.8	73.9	53.4
A6	4.12	25035.1	1220.9	1062.3
A7	4.39	19902.6	1046.9	835.9
A8	3.9	24439.4	1115.6	1172
A9	4.08	21321.1	1031.1	923.6
A10	4.25	14085.1	708.1	553.4

>TRINITY_DN103720_c0_g1_i12 from sample A4 / Example spliced isoform

CAGAGATGCTGAGCGGCAATGGCTACTCGTACAAGGTTGACATATGGAGCGCCGGAGTTGTGTTGTATGTTCTTCTCTGCGGGAAGAAACCTTTTGATGTGGAAACTGAAGACCCCAAGAGCATGTACCAGAAAATACAGAATACAGAAACAGATCTCAGTACTGGTCAATGGAGCTCCATATCCGAATCTGCAAAGAAA
CTCGTTCTTGGAATGTTGGAGAAGGGCTTCAACAACCGACTTTCTTGCGATCAAATTCTGAGTCATCCGTGGATCTTGAGCAATGCAGCAGAGATGGACATTGCTTCTGTTTCCACCGTCAACGCAGAGATGGGTTCTACCTCCGGGGACGATGCAACGGATTGAGTGACAATTCATATAAAAATAATATGATAATAAAG
AATGTAATAGAGGACCGCTTATATCATATATTTTGTGGTTGAATCATTTTGAACGACGAACTTGCTGGAGGCTTCGAATTAGTGTTTTATTGTTTTTTATTAATATACATGTTTCACTTTCATTTCTCCTAATTTCATCCCTGGTTTGCATTTTCCTAATCTAAGTTTCTATGTATGATGTAGCAATTTACATACCTAGT
TTATATGTATGATGCAGCAATTTACATATTTCGGTATCTCTAGGTATAGTTGTAAGATTTGATCATAATTGTTTTTATAAGTGGATTATAATGATTATAGTAGTATATAATTAAGTTTTATTAGTATTTTCATATTGAAATTGGGAATAAATTTG

>TRINITY_DN103720_c0_g1_i7 from sample A4 / Example unspliced isoform:

TTTCTTGCGATCAAATTCTGAgtgagtgggttatgatttgttttcaattttgcgaagttagttttttgaattggaattcggaagttctttgtgggattggaataaattagtgagtgaaggtaatgaaatattattgatgcagGTCATCCGTGGATCTTGAGCAATGCAGCAGAGATGGACATTGCTTCTGTTTCCACCGT
CAACGCAGAGATGGGTTCTACCTCCGGGGACGATGCAACGGATTGAGTGACAATTCATATAAAAATAATATGATAATAAAGAATGTAATAGAGGACCGCTTATATCATATATTTTGTGGTTGAATCATTTTGAACGACGAACTTGCTGGAGGCTTCGAATTAGTGTTTTATTGTTTTTTATTAATATACATGTTTCACTT
TCATTTCTCCTAATTTCATCCCTGGTTTGCATTTTCCTAATCTAAGTTTCTATGTATGATGTAGCAATTTACATACCTAGTTTATATGTATGATGCAGCAATTTACATATTTCGGTATCTCTAGGTATAGTTGTAAGATTTGATCATAATTGTTTTTATAAGTGGATTATAATGATTATAGTAGTATATAATTAAGTTTT
ATTAGTATTTTCATATTGAAATTGGGAATAAATTTG

Intron 4 GTCATCCCTG ... GGCAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.43	8	19	21
A2	60	10	13	17
A3	62.89	18	26	35
A4	70	3	6	8
A5	42.86	4	1	5
A6	58.69	101	124	163
A7	60.81	106	143	186
A8	58.78	176	216	286
A9	60.05	150	203	248
A10	62.21	99	144	182

>TRINITY_DN46042_c0_g2_i18 from sample A8 / Example spliced isoform

ACGGGGATATTCTGCTCCAGAGATGCTGCGTGGAAATGGGTACTCGTACGAGGTTGATATATGGAGCGCGGGAGTCGTGTTGTATGTTCTTCTCTGTGGTAATAAACCTTTTGTCGATGCCGAGGATGACATGATGATGTATGAACAAATACAGAATACAGAACTAGATCTCAGCAGGGGTGTATGGAGCTCCATATCTG
ATTAAGAAGCCTTTTGATGTGGAGAATGAATCTGGACCAAAGGAACTCGTTCTTGGAATGTTGGAGAAAGACTGCACGCAGCGACTTTCTTGCGATGAAATTCTGTCAGAGATGGACGTGGCTTCTACTTCCACCATCAACGCGGAGATGGGTTGTACCTCGTGGAGCGATGCATCCCAGCAACAAATACATAGAATTCA
GAGACAATACATATCATCCGATAAGAATAAGAATGTAATAGAGGATTAGAGACTGAAGGATTACCAAGATCGATCGATAAGATTTTTTTGTGGATGAATGATTTTGAACGACCTCCGCCTCTGGAACTTGCTGGAGGCTTTCAATGAGTTTTTGTTTTCATTTGGTTATATGGCCCTTTATTGTATGAAATTTAATGTTG
GGTAAAATAATGAAATATAATACAGTATGCT

>TRINITY_DN46042_c0_g2_i26 from sample A8 / Example unspliced isoform:

ACGGGGATATTCTGCTCCAGAGATGCTGCGTGGAAATGGGTACTCGTACGAGGTTGATATATGGAGCGCGGGAGTCGTGTTGTATGTTCTTCTCTGTGGTAATAAACCTTTTGTCGATGCCGAGGATGACATGATGATGTATGAACAAATACAGAATACAGAACTAGATCTCAGCAGGGGTGTATGGAGCTCCATATCTG
ATTAAGAAGCCTTTTGATGTGGAGAATGAATCTGGACCAAAGGAACTCGTTCTTGGAATGTTGGAGAAAGACTGCACGCAGCGACTTTCTTGCGATGAAATTCTGTgtcatccctggatctcgggcaatgcagCAGAGATGGACGTGGCTTCTACTTCCACCATCAACGCGGAGATGGGTTGTACCTCGTGGAGCGATGC
ATCCCAGCAACAAATACATAGAATTCAGAGACAATACATATCATCCGATAAGAATAAGAATGTAATAGAGGATTAGAGACTGAAGGATTACCAAGATCGATCGATAAGATTTTTTTGTGGATGAATGATTTTGAACGACCTCCGCCTCTGGAACTTGCTGGAGGCTTTCAATGAGTTTTTGTTTTCATTTGGTTATATGG
CCCTTTATTGTATGAAATTTAATGTTGGGTAAAATAATGAAATATAATACAGTATGCT

Intron 5 GTGAGTGGGT ... ATTGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	12.47	1057.5	150	57.5
A2	12.8	940	138	61
A3	14.43	1073.5	181	66
A4	11.74	692.5	92	51.5
A5	13.31	560.5	86	27.5
A6	12.11	12028.5	1654	595.5
A7	14.35	10311.5	1726	586.5
A8	13.27	11904.5	1818.5	794
A9	13.96	10726.5	1738	595
A10	15.79	7242	1357.5	442.5

>TRINITY_DN42316_c0_g1_i7 from sample A9 / Example spliced isoform

ACATTGGGATTTTCTGCTCCAGAGATGCTGAGCGGCAATGGCTACTCGTACAAAGTTGACATATGGAGCGCCGGAGTTGTGTTGTATGTTCTTCTCTGTGGGAAGAAGCCTTTTGATGTGGAGAATGAAGACCCGAAGAGCATGTACGAGAAAATACAGAATACAGAGCCAGATCTCAGTACTGGCGTATGGAGCTCCAT
ATCCGAATCTACAAAGGAACTCGTTCTTGGAATGTTGAAGAAAAACTTGGGCCAACGACTTTCTTGGGATGAAATTCTGAGTCATCCGTGGATCTTGAGCAATGCAGCAGAGACAGAGATGGACATTGCTTCTGTTTCCACCGTCAACGCAGAGATGGGTTCTACCACTTCTACCTCCGCGGACGATGCAACGAATTGAG
TGAGTTCTACCTTCCGGGACAATGCAACGAATTGGGTGACAATAAAATACATATAATCTGATAGTAAAGAATGTAATAGATTCATATATTTTGTGGTAATTGAATGATTTTGAACGACGAACTTCTGGAGGTTTCCAAGTGTTTTATTGTTTTTTCTTAATATACATGTTTCGCTTTGTTTTCATTTCTGCTAATTTCAT
CTCTGATTGGCATTTTCCTAATCTAAGTTTCTATTTATAACAATTTACATACCC

>TRINITY_DN42316_c0_g1_i8 from sample A9 / Example unspliced isoform:

ACATTGGGATTTTCTGCTCCAGAGATGCTGAGCGGCAATGGCTACTCGTACAAAGTTGACATATGGAGCGCCGGAGTTGTGTTGTATGTTCTTCTCTGTGGGAAGAAGCCTTTTGATGTGGAGAATGAAGACCCGAAGAGCATGTACGAGAAAATACAGAATACAGAGCCAGATCTCAGTACTGGCGTATGGAGCTCCAT
ATCCGAATCTACAAAGGAACTCGTTCTTGGAATGTTGAAGAAAAACTTGGGCCAACGACTTTCTTGGGATGAAATTCTGAgtgagtgggttatgatttgctttcaattttgcatattagttttgtgaattggaattcagaagttctttttgcgattggaattaattagtgactgaacgtaatggaatattattgatgcag


GTCATCCGTGGATCTTGAGCAATGCAGCAGAGACAGAGATGGACATTGCTTCTGTTTCCACCGTCAACGCAGAGATGGGTTCTACCACTTCTACCTCCGCGGACGATGCAACGAATTGAGTGAGTTCTACCTTCCGGGACAATGCAACGAATTGGGTGACAATAAAATACATATAATCTGATAGTAAAGAATGTAATAGA
TTCATATATTTTGTGGTAATTGAATGATTTTGAACGACGAACTTCTGGAGGTTTCCAAGTGTTTTATTGTTTTTTCTTAATATACATGTTTCGCTTTGTTTTCATTTCTGCTAATTTCATCTCTGATTGGCATTTTCCTAATCTAAGTTTCTATTTATAACAATTTACATACCC