sp|Q9XER8|RAB7_GOSHI ----> Ras-related protein Rab7

Intron 1 GTAAGTGGGT ... AATATGGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.62	11	10	9
A2	42.86	8	6	7
A3	55.88	15	19	13
A4	62.96	10	17	20
A5	38.46	8	5	5
A6	51.72	28	30	3
A7	46.38	37	32	2
A8	55.88	15	19	0
A9	56.52	30	39	2
A10	49.33	38	37	0

>TRINITY_DN113285_c0_g2_i4 from sample A3 / Example spliced isoform

AGTTTTCCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTGAGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCACGGCTTGCTCA
TCTCGGTAGTATCAATCTCATCACGTCTATCGTAAGTACAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGTACCTGGAACACAGTTTAGCCAGTTTTGTTTCTTTTGGACTGTTGAATTCGGTGGATCGGTGTCAGGGTTTTCAGCCAGCTTGGAAATTGACATTTGGG
TTCTGTTGATTTTTTGTGGGCAAGGT

>TRINITY_DN113285_c0_g2_i2 from sample A3 / Example unspliced isoform:

GTGAGCACTCATCCTACAAATGGGACAGATGGAGAGCTTTGAAATCCATGCATCCGCGCATGGAACGTGTGGCTACATACCGGAATCACGATGCACTACTCCCCACTCTGGAAAGCGAGCCTCGAGCCACACCGCACACTCAGTGTTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTCG
ATGGTGGGGAGCTGGTGGAGGTCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTCTGCTGGTCTTTCTTGTGGTCTCTGTTGTACATAAATATACAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCGCAATTATAATAGTTTT
CCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTGAGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCATGGCTTGCTCATCTCGG
TAGTATCAATCTCATCACGTCTATCACAAGTTCAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGgtaagtgggttatgtaatttagcaattcttccatctgatgaatctgatttcaagttttctgcttcaaatagctcgatttgaaggccatattgctaggttgtgtta
aattaggggtttgattgaattttaggtttgtaaatggtggttattttgtttcaatatggtagTACCTGGAACACAGTTTAGCCAGTTTTGTTTCTTTTGGACTGTTGAATTCGGTGGATCGGTGTCAGGGTTTTCAGCCAGCTTGGAAATTGACATTTGGGTTCTGTTGATTTTTTGTGGGCAAGGTTGGTAAAACCTCG
TTGATGAATCAATATGTAAACAAGAAATTTAATAACCAGTATAAAGCAACCATTGGAGCGGATTTCTTGACAAAAGAAGTTCAATTTGAGGATGGATTGTTCACTTTACATGTACTTACTGCTTGGAAATAAA

Intron 2 GTAGGTATAT ... AGTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.49	182.3	4	184
A2	46.75	149.7	1.3	102
A3	53.74	187.7	1.7	170.3
A4	50.33	109.3	7	86
A5	48.33	97.7	3.7	70.7
A6	54.54	1138.7	2.3	932.3
A7	52.05	1045	2	793
A8	55.38	1147.3	1	1009.7
A9	53.21	1099.7	4.3	881.7
A10	56.15	810.7	1	729.7

>TRINITY_DN118930_c1_g1_i2 from sample A2 / Example spliced isoform

CGTGGGCACTCATCCTACAAATGGGACAGATGGAGAGCTTTGCAATCCATGCATCCGCGCATGGAACGTGTGGCTACATACCGGAATCACGATGCACTACTCCCCACTCTGGAAAGCGAGCCTCGAGCCACACCGCACACTCAGTGTTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTC
GATGGTGGGGAGCTGGTGGAGGTCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTCTGCTGGTCTTTCTTGTGGTCTCTGTTGTACATAAATATACAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCGCAATTATAATAGTTT
TCCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTGAGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCATGGCTTGCTCATCTCG
GTAGTATCAATCTCATCACGTCTATCACAAGTTCAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGGTACTTACTGCTTGGAAATAAAATTGATGTGGATGGTGGGAACAGCAGAGTGGTTTCTGAGAAAAAGGCCAGGGCATGGTGTGCTTCTAAAGGAAACATTCCCT
ACTTCGAAACTTCTGCAAAAGAGGGCTTTAATGTTGAAGCAG

>TRINITY_DN118930_c1_g1_i13 from sample A2 / Example unspliced isoform:

GATGGTGGGAACAGCAGAGTGgtaggtatatgtatatttcatttagttattcctctggagtcaatgtttaaactcttcgatatgtacttaaactcttcagtttttcagGTTTCTGAGAAAAAGGCCAGGGCATGGTGTGCTTCTAAAGGAAACATTCCCTACTTCGAAACTTCTGCAAAAGAAGGCTTTAATGTTGAAGC
AGCTTTCCATTGTATAGCCAAGAATGCATTGGAGAACGAACCTGAAGAGGAGATCTTAATATCTGGACACAATTGATGTCAATGTTTGGAGACCACAAAGAGCATCAGGATGTGATTGTTAGTCGCCAGGGGATCATACAAGACTTTGATGCTACAAATAATAGTTTACTTGCATGGTAAGATATAGACATTAAATCAGG
CCATTGAGATGCGGATCAAACATTGATTTGTGTAGCCAGTGTAGAAATAGAATTGTACTCTAGTAATGCTTATTAAAAATTAGCTCTTTGTTTCACAATTTCGCAATTCAGTTCTTCAAGGATGGATTACCAACATTTACTACGATTCATATGGTGATTACTCTGAAATTGCATTTCACTTGCAAAATATACTGCTTTTT
TATGCCATTATTCTATTGTGATTATTTTAAAATATGGCTCCAATTATTGTTTGATTGTTTGCCATGAAAATAAGAAGCTAAATGATTCCAAACTTCCAATGTAGATTGATTTAT

Intron 3 GTAAGTGGGT ... TGTGGGCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.2	121	10	78
A2	36.53	106	6	61
A3	40.48	100	19	68
A4	40.91	78	17	54
A5	46.75	41	5	36
A6	36.48	357	30	205
A7	36.57	392	32	226
A8	34.21	300	19	156
A9	36.31	428	39	244
A10	37.5	325	37	195

>TRINITY_DN113285_c0_g2_i3 from sample A3 / Example spliced isoform

GTGAGCACTCATCCTACAAATGGGACAGATGGAGAGCTTTGAAATCCATGCATCCGCGCATGGAACGTGTGGCTACATACCGGAATCACGATGCACTACTCCCCACTCTGGAAAGCGAGCCTCGAGCCACACCGCACACTCAGTGTTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTCG
ATGGTGGGGAGCTGGTGGAGGTCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTCTGCTGGTCTTTCTTGTGGTCTCTGTTGTACATAAATATACAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCGCAATTATAATAGTTTT
CCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTGAGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCATGGCTTGCTCATCTCGG
TAGTATCAATCTCATCACGTCTATCACAAGTTCAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGGTTGGTAAAACCTCGTTGATGAATCAATATGTAAACAAGAAATTCAATAACCAGTATAAATCAACCATTGGAGCAGATTTCTTGACAAAAGAAGTTCAATTTGAG
GATGGATTGTTCACTTTACATATATGGGATACTGCTGGCCAAGAAAGATTTCAGAGCCTTGGTGTTGCCTTCTACCGTGGTACTTACTGCTTGGAAATAA

>TRINITY_DN113285_c0_g2_i1 from sample A3 / Example unspliced isoform:

GTGAGCACTCATCCTACAAATGGGACAGATGGAGAGCTTTGAAATCCATGCATCCGCGCATGGAACGTGTGGCTACATACCGGAATCACGATGCACTACTCCCCACTCTGGAAAGCGAGCCTCGAGCCACACCGCACACTCAGTGTTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTCG
ATGGTGGGGAGCTGGTGGAGGTCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTCTGCTGGTCTTTCTTGTGGTCTCTGTTGTACATAAATATACAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCGCAATTATAATAGTTTT
CCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTGAGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCATGGCTTGCTCATCTCGG
TAGTATCAATCTCATCACGTCTATCACAAGTTCAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGgtaagtgggttatgtaatttagcaattcttccatctgatgaatctgatttcaagttttctgcttcaaatagctcgatttgaaggccatattgctaggttgtgtta
aattaggggtttgattgaattttaggtttgtaaatggtggtttttttgttttaatatggtagtccctggaacgcagtttagccagttttgtttattttggactgctaaattcggtggatcggcgtcagggttttcagccagattggaaattgacatttgggttctgttgattttttgtgggcaagGTTGGTAAAACCTCG
TTGATGAATCAATATGTAAACAAGAAATTTAATAACCAGTATAAAGCAACCATTGGAGCGGATTTCTTGACAAAAGAAGTTCAATTTGAGGATGGATTGTTCACTTTACATATATGGGATACTGCTGGCCAAGAAAGATTTCAGAGCCTTGGTGTTGCCTTCTACCGTGGTACTTACTGCTTGGAAATAA

Intron 4 GTATCCTGGA ... GCCTTCATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.09	447	77.3	54.5
A2	8.39	442	88.3	44.8
A3	9.88	436	93.3	51.8
A4	7.1	383	66.3	32.3
A5	8.61	210	38.5	22.5
A6	6.33	2128	432	164.5
A7	5.59	1991	404.8	136
A8	5.96	1371	274.5	99.5
A9	6.39	2183.8	396	169.5
A10	5.9	1899	352.5	140.8

>TRINITY_DN107706_c0_g1_i1 from sample A4 / Example spliced isoform

CTTCCCCCGGCTTCGAGTGTACTCAAGTGTACAAACTTAATTTTAATTCCCTGATCAATGACACCCTGGCATATGCTTTCAAAATTGATTGCCAAGACATATATTAATCCCCAATTTTATTTTTAACATTATACGAAACGAGGGGAGCGATCAATTGACCAGAGGAGGGCTTAAGCTAAAAACTTCAGCCAAGGCAGTTT
AGATCTCTCCGACTCCAATTTTATCGATCGTAAGCTCGATCTCATCATGTCTGCCCGCAGAAGAACTCTGTTAAAGGTCATCATCCTTGGCGATAGCGGGGTCGGTAAAACCTCGCTGATGAATCAATATGTAAACAAGAAATTCAGCAACCAGTATAAAGCAACCATTGGAGCAGATTTTTTGACGAAAGAAGTGCAGT
TTGAGGATAGGTTGTTCACTTTACAGATATGGGATACCGCTGGCCAGGAAAGATTTCAGAGCCTTGGTGTTGCCTTCTACCGAGGTGCTGATTGCTGTGTTCTTGTTTACGATGTTAACGTGCTAAAATCATTTGATAACCTAAATAATTGGCGCGATGAATTTCTAATTCAGGTATTTGCTCGATACACTTTTGAGTAT
TAAAACATATTACAACGTGTTTCTCTTGACTTGTGCTAATCTACTTAACCATTTGATCTGTATAGGCA

>TRINITY_DN107706_c0_g1_i6 from sample A4 / Example unspliced isoform:

CTTCCCCCGGCTTCGAGTGTACTCAAGTGTACAAACTTAATTTTAATTCCCTGATCAATGACACCCTGGCATATGCTTTCAAAATTGATTGCCAAGACATATATTAATCCCCAATTTTATTTTTAACATTATACGAAACGAGGGGAGCGATCAATTGACCAGAGGAGGGCTTAAGCTAAAAACTTCAGCCAAGGCAGTTT
AGATCTCTCCGACTCCAATTTTATCGATCGTAAGCTCGATCTCATCATGTCTGCCCGCAGAAGAACTCTGTTAAAGGTCATCATCCTTGGCGATAGCGGGGTCGGTAAAACCTCGCTGATGAATCAgtatcctggaattgatttcgatatctagtgttttgttttgaatttgttttattatgtattctgggtgatctatt
tagggtttgtcaccgccggttagattaggttaaagctatgttggaattagaattttaaattcaacgtaacaccacgcagctttgaaaatactaattttaattatatttaactagtgtggtccttaagtgccttcatagATATGTAAACAAGAAATTCAGCAACCAGTATAAAGCAACCATTGGAGCAGATTTTTTGACGA
AAGAAGTGCAGTTTGAGGATAGGTTGTTCACTTTACAGGTGAGGAATAGTTACCCCATGTGTATTACCATTTCCTTTAAAGAGGAAGGCTTAAGCATGCGATTGTTGTGAAATAAAATAATTAAAGCGGTTAGCTGGTTTTCGGAGGGTGTTGTGGAGCGCTTCTGAGAAGTTTTTGCAATCCAGCTATTGTGATTTAAG
ATCTATGTGAATTTTCACAACTCATTACTATCTATACTCCCATGCAATATCTGGATCTATTGTAGGAAGGTCGGGTCATAGTCATTTAATG

Intron 5 GTGTTGCCTT ... TATAATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	160	94
A2	100	0	154	60
A3	99.27	1	177	95
A4	100	0	139	68
A5	100	0	47	34
A6	99.16	6	1019	397
A7	99.53	3	920	341
A8	99.2	4	711	275
A9	98.91	7	893	372
A10	99.6	2	701	284

>TRINITY_DN45230_c0_g3_i6 from sample A6 / Example spliced isoform

GGGACAGATGGAGAGCTTTGCAATCCATGCATCCGCGCATGGAACGTGTGGCTACATACCGGAATCACGATGCACTACTCCCCACTCTGGAAAGCGAGCCTCGAGCCACACCGCACACTCAGTGCTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTCGATGGTGGGGAGCTGGTGGAGG
TCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTCTGATGGTCTTTCTTGTGGTCTCTGTGGTACATAAATATACAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCTTAGCTATAACAGTTTTCCCTATCATTGCCCGCCTATA
ATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTGAGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCATGGCTTGCTCATCTCGGTAGTATCAATCTCATCACGTC
TATCACAAGTTCAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGATATGGGATACTGCTGGCCAAGAAAGATTTCAGAGCCTTGGTACTTACTGCTTGGAAATAA

>TRINITY_DN45230_c0_g3_i13 from sample A6 / Example unspliced isoform:

GGGACAGATGGAGAGCTTTGCAATCCATGCATCCGCGCATGGAACGTGTGGCTACATACCGGAATCACGATGCACTACTCCCCACTCTGGAAAGCGAGCCTCGAGCCACACCGCACACTCAGTGCTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTCGATGGTGGGGAGCTGGTGGAGG
TCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTCTGATGGTCTTTCTTGTGGTCTCTGTGGTACATAAATATACAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCGCAATTATAATAGTTTTCCCTATCATTGCCCGCCTATA
ATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTGAGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCATGGCTTGCTCATCTCGGTAGTATCAATCTCATCACGTC
TATCACAAGTTCAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGATATGGGATACTGCTGGCCAAGAAAGATTTCAGAGCCTTGgtgttgccttctaccgtggtgctgattgatgtgttcttgtttacaatgttaacgtgctaaaatcattcgataacctaaataattgg
tgtgatgaatttataattcagGTACTTACTGCTTGGAAATAAAATTGATGTGGATGGTGGGAACAGCAGAGTGGTTTCTGAGAAAAAGGCCA

Intron 6 GTATCCTGGA ... GCCTTCATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.85	447	3	132.5
A2	21.98	442	4	124.5
A3	21.98	436	7	123
A4	18.75	383	6	88.5
A5	23.58	210.5	9	65
A6	20.23	2130.5	3	540.5
A7	19.41	1994	2	480.5
A8	19.49	1371	3	332
A9	20.17	2185	7	552.5
A10	20.96	1899	2	504

>TRINITY_DN48732_c0_g1_i13 from sample A7 / Example spliced isoform

CTTCCCCCGGCTTCGAGTGTACTCAAGTGTACAAACTTAATTTTAATTCCCTGATCAATGACACCCTGGCATATGCTTTCAAAATTGATTGCCAAGACATATATTAATCCCCAATTTTATTTTTAACATTATACGAATCGAGGGGAGCGATCAATTGACCAGGGGAGGGCTTAAGCTAAAAACTTCAGCCAAGGCAGTTT
AGATCTCTACGACTCCAATTTTATCGATCGTAAGCTCGATCTCATCATGTCTGCCCGCAGAAGAACTCTGTTAAAGGTCATCATCCTTGGCGATAGCGGGGTCGGTAAAACCTCGCTGATGAATCAATATGTAAACAAGAAATTCAGCAACCAGTATAAAGCAACCATTGGAGCAGATTTTTTGACGAAAGAAGTGCAGT
TTGAGGATAGGTTGTTCACTTTACAGATATGGGATACTGCTGGCCAAGAAAGATTTCAGAGCCTTGGTGTTGCCTTCTACCGAGGTGCTGATTGCTGTGTTCTTGTTTACGATGTTAACGTGCTAAAATCATTTGATAACCTAAATAATTGGCGCGATGAATTTCTAATTCAGGCAAGCCCTTCTGACCCAGAAAACTTT
CCATTTGTTGTGCTTGGAAATAAAATCGACGTGGATGGTGGGAACAGCAGAGTGGTTTCTGAGAAAAAGGCCAAGGCATGGTGTGCTTCTAAAGGCAACATTCCCTACTTCGAAACTTCTGCAAAAGAGGGCTTTAATGTCGAAGCAGCTTTCCAGTGTATAGCCAAGAATGCGTTGAAGAACGAGCCTGAAGAGGAGAT
ATATCTGCCGGACACAATTGATGTCAATGCTGGGAGACCACAAAGAGCATCAGGATGCGATTGTTAGTCAC

>TRINITY_DN48732_c0_g1_i1 from sample A7 / Example unspliced isoform:

GGTAAAACCTCGCTGATGAATCAgtatcctggaattgatttcgatatctagtgttttgaatttgttttattatgtattctgggtgatctatttagggtttgtcactgcctgttagattaggttaaagttatgttggaattgaaattttaaattcaacgtaacaccacgcagctttgaaaatactaattttaattatattt
aactagtgtggtccttaagtgccttcatagATATGTAAACAAGAAATTCAGCAACCAGTATAAAGCAACCATTGGAGCAGATTTTTTGACGAAAGAAGTGCAGTTTGAGGATAGGTTGTTCACTTTACAGATATGGGATACTGCTGGCCAAGAAAGATTTCAGAGCCTTGGTGTTGCCTTCTACCGAGGTGCTGATTGCT
GTGTTCTTGTTTACGATGTTAACGTGCTAAAATCATTTGATAACCTAAATAATTGGCGCGATGAATTTCTAATTCAGGCAAGCCCTTCTGACCCAGAAAACTTTCCATTTGTTGTGCTTGGAAATAAAATCGACGTGGATGGTGGGAACAGCAGAGTGGTTTCTGAGAAAAAGGCCAAGGCATGGTGTGCTTCTAAAGGC
AACATTCCCTACTTCGAAACTTCTGCAAAAGAGGGCTTTAATGTCGAAGCAGCTTTCCAGTGTATAGCCAAGAATGCGTTGAAGAACGAGCCTGAAGAGGAGATATATCTGCCGGACACAATTGATGTCAATGCTGGGAGACCACAAAGAGCATCAGGATGCGATTGTTAGTCAC

Intron 7 GTAAGTGGGT ... TCGGTGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.71	19	10	14
A2	33.33	15	6	9
A3	36.36	35	19	21
A4	50	21	17	25
A5	36	8	5	4
A6	27.55	71	30	24
A7	25.76	85	32	27
A8	28.21	42	19	14
A9	25.86	86	39	21
A10	26.36	81	37	21

>TRINITY_DN113285_c0_g2_i7 from sample A3 / Example spliced isoform

GTGAGCACTCATCCTACAAATGGGACAGATGGAGAGCTTTGAAATCCATGCATCCGCGCATGGAACGTGTGGCTACATACCGGAATCACGATGCACTACTCCCCACTCTGGAAAGCGAGCCTCGAGCCACACCGCACACTCAGTGTTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTCG
ATGGTGGGGAGCTGGTGGAGGTCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTCTGCTGGTCTTTCTTGTGGTCTCTGTTGTACATAAATATACAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCGCAATTATAATAGTTTT
CCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTGAGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCATGGCTTGCTCATCTCGG
TAGTATCAATCTCATCACGTCTATCACAAGTTCAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGGGTTTTCAGCCAGCTTGGAAATTGACATTTGGGTTCTGTTGATTTTTTGTGGGCAAGGTTGGTAAAACCTCGTTGATGAATCAATATGTAAACAAGAAATTCAAT
AACCAGTATAAATCAACCATTGGAGCAGATTTCTTGACAAAAGAAGTTCAATTTGAGGATGGATTGTTCACTTTACATGTACTTACTGCTTGGAAATAAA

>TRINITY_DN113285_c0_g2_i2 from sample A3 / Example unspliced isoform:

GTGAGCACTCATCCTACAAATGGGACAGATGGAGAGCTTTGAAATCCATGCATCCGCGCATGGAACGTGTGGCTACATACCGGAATCACGATGCACTACTCCCCACTCTGGAAAGCGAGCCTCGAGCCACACCGCACACTCAGTGTTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTCG
ATGGTGGGGAGCTGGTGGAGGTCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTCTGCTGGTCTTTCTTGTGGTCTCTGTTGTACATAAATATACAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCGCAATTATAATAGTTTT
CCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTGAGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCATGGCTTGCTCATCTCGG
TAGTATCAATCTCATCACGTCTATCACAAGTTCAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGgtaagtgggttatgtaatttagcaattcttccatctgatgaatctgatttcaagttttctgcttcaaatagctcgatttgaaggccatattgctaggttgtgtta
aattaggggtttgattgaattttaggtttgtaaatggtggttattttgtttcaatatggtagtacctggaacacagtttagccagttttgtttcttttggactgttgaattcggtggatcggtgtcagGGTTTTCAGCCAGCTTGGAAATTGACATTTGGGTTCTGTTGATTTTTTGTGGGCAAGGTTGGTAAAACCTCG
TTGATGAATCAATATGTAAACAAGAAATTTAATAACCAGTATAAAGCAACCATTGGAGCGGATTTCTTGACAAAAGAAGTTCAATTTGAGGATGGATTGTTCACTTTACATGTACTTACTGCTTGGAAATAAA

Intron 8 GTAAGTGGGT ... TCGGTGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.7	19	10	13
A2	31.82	15	5	9
A3	35.78	35	17	22
A4	50	21	16	26
A5	36	8	5	4
A6	27.55	71	25	29
A7	24.66	84	28	27
A8	28.57	40	17	15
A9	25	84	36	20
A10	25.79	82	36	21

>TRINITY_DN107706_c0_g1_i14 from sample A4 / Example spliced isoform

AGCGAGCCTCGAGCCACACCGCACACTCAGTGTTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTCGATGGTGGGGAGCTGGTGGAGGTCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTTTGATGGTCTTTCTTGTGGTCTCTGTGGTACATAAATATA
CAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCTTAGCTATAACAGTTTTCCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTG
AGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCACGGCTTGCTCATCTCGGTAGTATCAATCTCATCACGTCTATCATAAGTACAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGGGTTTTCAGCCAGCTTGG
AAATTGACATTTGGGTTCTGTTGATTTTTTGTGGGCAAGGTTGGTAAAACCTCGTTGATGAATCAATATGTAAACAAGAAATTCAATAACCAGTATAAATCAACCATTGGAGCAGATTTCTTGACAAAAGAAGTTCAATTTGAGGATGGATTGTTCACTTTACATGTGAGGAATAGTTACCCCATGTGTATTACCATTTC
CTTTAAAGAGGAAGGCTTAAGCATGCGATTGTTGTGAAATAAAATAATTAAAGCGGTTAGCTGGTTTTCGGAGGGTGTTGTGGAGCGCTTCTGAGAAGTTTTTGCAATCCAGCTATTGTGATTTAAGATCTATGTGAATTTTCACAACTCATTACTATCTATACTCCCATGCAATATCTGGATCTATTGTAGGAAGGTCG
GGTCATAGTCATTTAATG

>TRINITY_DN107706_c0_g1_i15 from sample A4 / Example unspliced isoform:

AGTTTTCCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTGAGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCACGGCTTGCTCA
TCTCGGTAGTATCAATCTCATCACGTCTATCATAAGTACAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGgtaagtgggttatgtaatttagcaattcttccatctgatgaatctgatttcaagttttctgcttcaaatagctcgatttgaaggccatattgctaggtt
gtgttaaattagggatttgaattgaattttaggtttgtaaatggtggttattttgtttcaatatggtagtacctggaacacagtttagccagttttgtttcttttggactgttgaattcggtggatcggtgtcagGGTTTTCAGCCAGCTTGGAAATTGACATTTGGGTTCTGTTGATTTTTTGTGGGCAAGGTTGGTAA
AACCTCGTTGATGAATCAATATGTAAACAAGAAATTCAATAACCAGTATAAATCAACCATTGGAGCAGATTTCTTGACAAAAGAAGTTCAATTTGAGGATGGATTGTTCACTTTACATGTACTTACTGCTTGGAAATAAAATTGATGTGGATGGTGGGAACAGCAGAGTGGT

Intron 9 GTAAGTGGGT ... TGTGGGCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.46	39.5	10	23
A2	36.26	22	5	20
A3	48.77	30.5	17	41
A4	53.49	20	16	30
A5	41.43	8.5	5	7
A6	35.15	77.5	25	59
A7	36.51	116.5	28	106
A8	37.95	60.5	17	57
A9	39.72	82	36	72
A10	37.46	83.5	36	64

>TRINITY_DN107706_c0_g1_i17 from sample A4 / Example spliced isoform

AGCGAGCCTCGAGCCACACCGCACACTCAGTGTTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTCGATGGTGGGGAGCTGGTGGAGGTCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTCTGCTGGTCTTTCTTGTGGTCTCTGTTGTACATAAATATA
CAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCGCAATTATAATAGTTTTCCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTG
AGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCACGGCTTGCTCATCTCGGTAGTATCAATCTCATCACGTCTATCATAAGTACAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGGTTGGTAAAACCTCGTTG
ATGAATCAATATGTAAACAAGAAATTCAATAACCAGTATAAAGCAACAATTGGAGCAGATTTCTTGACAAAAGTTCAATTTGAGGATGGATTGTTCACTTTACATGTGAGGAATAGTTACCCCATGTGTATTACCATTTCCTTTAAAGAGGAAGGCTTAAGCATGCGATTGTTGTGAAATAAAATAATTAAAGCGGTTAG
CTGGTTTTCGGAGGGTGTTGTGGAGCGCTTCTGAGAAGTTTTTGCAATCCAGCTATTGTGATTTAAGATCTATGTGAATTTTCACAACTCATTACTATCTATACTCCCATGCAATATCTGGATCTATTGTAGGAAGGTCGGGTCATAGTCATTTAATG

>TRINITY_DN107706_c0_g1_i15 from sample A4 / Example unspliced isoform:

AGTTTTCCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTGAGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCACGGCTTGCTCA
TCTCGGTAGTATCAATCTCATCACGTCTATCATAAGTACAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGgtaagtgggttatgtaatttagcaattcttccatctgatgaatctgatttcaagttttctgcttcaaatagctcgatttgaaggccatattgctaggtt
gtgttaaattagggatttgaattgaattttaggtttgtaaatggtggttattttgtttcaatatggtagtacctggaacacagtttagccagttttgtttcttttggactgttgaattcggtggatcggtgtcagggttttcagccagcttggaaattgacatttgggttctgttgattttttgtgggcaagGTTGGTAA
AACCTCGTTGATGAATCAATATGTAAACAAGAAATTCAATAACCAGTATAAATCAACCATTGGAGCAGATTTCTTGACAAAAGAAGTTCAATTTGAGGATGGATTGTTCACTTTACATGTACTTACTGCTTGGAAATAAAATTGATGTGGATGGTGGGAACAGCAGAGTGGT

Intron 10 GTGCTGATTG ... TATAATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76.76	52	220.3	113.3
A2	79.53	37	206.3	76.7
A3	78.51	49	244.3	115.3
A4	82.19	30	188	81.7
A5	73.68	22	77.7	44.3
A6	81.89	227.7	1428.3	509.7
A7	76.58	268.3	1277.7	459.3
A8	73.74	245	1006	378
A9	77.08	263.7	1256	493
A10	76.22	220.3	1014.7	378.7

>TRINITY_DN107706_c0_g1_i2 from sample A4 / Example spliced isoform

AGCGAGCCTCGAGCCACACCGCACACTCAGTGTTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTCGATGGTGGGGAGCTGGTGGAGGTCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTCTGCTGGTCTTTCTTGTGGTCTCTGTTGTACATAAATATA
CAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCGCAATTATAATAGTTTTCCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTG
AGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCACGGCTTGCTCATCTCGGTAGTATCAATCTCATCACGTCTATCATAAGTACAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGTACCTTGAACACAGTTTA
GCCAGTTTTGTTTCTTTTGGACTGTTGAATTCGGTGGATCGGTGTCAGGGTTTTCAGCCAGCTTGGAAATTGACATTTGGGTTCTGTTGATTTTTTGTGGGCAAGGTTGGTAAAACCTCGTTGATGAATCAATATGTAAACAAGAAATTCAATAACCAGTATAAATCAACCATTGGAGCAGATTTCTTGACAAAAGAAGT
TCAATTTGAGGATGGATTGTTCACTTTACATATATGGGATACCGCTGGCCAGGAAAGATTTCAGAGCCTTGGTGTTGCCTTCTACCGTGGTACTTACTGCTTGGAAATAAAATTGATGTGGATGGTGGGAACAGCAGAGTGGTTTCTGAGAAAAAGGCCAGGGCATGGTGTGCTTCTAAAGGCAACATTCCCTACTTCGA
AACTTCTGCAAAAGAGGGCTTTAATGTTGAAGCAGCTTTCCATTGTATAGCCAAGAATGCATTGGAGAACGAGCCTGAAGAGGAGATATATCTGGACACATTTGATGTCAATGTTGGGAGACCACAAAGAGCATCAGGATACGATTGTTAGTCACCAGGGGATCATTCAAGACTTTGATGCTGCAAATAATAGTTTACTT
GCATGAAATTCAGAGAAATAAAGTATACAGTCTCAATTTGTCCCTGGATCAAATGGAAGCACAGTCAACAGGAGCCCAGATGAAATTGCGTCTCATGTTTAAGCTGTTCAAAAAATGGAAGGATGAGGTGCACTGAAATTAGCATTGGGAATTCGTTGGGATCATAAAATGCGTGAAACATGTTTATGAATCCGATTCTA
ATTCTTATTCCTAGGCGATAACAGTTTGAGGAAAGTGAGAATTCAGATGCAGGCTGGTGTTCTCATTCAAATGGCT

>TRINITY_DN107706_c0_g1_i3 from sample A4 / Example unspliced isoform:

AGCGAGCCTCGAGCCACACCGCACACTCAGTGTTGCAACTTGTCGTATCCCTGAGTCCCTTCTTCTCCTCTTTCTTTACAAAATTCGATGGTGGGGAGCTGGTGGAGGTCAGCCCCTGAGAGCCCCTTCTGCTTGGGAGGATTTCTGTTTTTGTTGTCCTCTGCTGGTCTTTCTTGTGGTCTCTGTTGTACATAAATATA
CAAAACGAGCCACAGATACCCCACCACCATCATCCTGATGGCCGCTCAGAGCAACAGCAGCGACACCACCGCAATTATAATAGTTTTCCCTATCATTGCCCGCCTATAATGATAACGTACTCCCAAATAGAAATGCCTGTATTGAGCGAAGCCTCTGCTATTATTTCAAGAAAATCTGATCTCACAGACGGACATAATTG
AGTAGCCAGACCATTGAATCCTTCATATTGCAGCAGAGCAAGACAGAATATGGACGGTCTGCCAATGCCACGGCTTGCTCATCTCGGTAGTATCAATCTCATCACGTCTATCATAAGTACAAGCTCATCATGTCTATGTGCAGAAGAACTTTGTTAAAGGCCACCATCCTTGGCGATAACAGTACCTTGAACACAGTTTA
GCCAGTTTTGTTTCTTTTGGACTGTTGAATTCGGTGGATCGGTGTCAGGGTTTTCAGCCAGCTTGGAAATTGACATTTGGGTTCTGTTGATTTTTTGTGGGCAAGGTTGGTAAAACCTCGTTGATGAATCAATATGTAAACAAGAAATTCAATAACCAGTATAAATCAACCATTGGAGCAGATTTCTTGACAAAAGAAGT
TCAATTTGAGGATGGATTGTTCACTTTACATATATGGGATACCGCTGGCCAGGAAAGATTTCAGAGCCTTGGTGTTGCCTTCTACCGTGgtgctgattgatgtgttcttgtttacaatgttaacgtgctaaaatcattcgataacctaaataattggtgtgatgaatttataattcagGTACTTACTGCTTGGAAATAAA
ATTGATGTGGATGGTGGGAACAGCAGAGTGGTTTCTGAGAAAAAGGCCAGGGCATGGTGTGCTTCTAAAGGCAACATTCCCTACTTCGAAACTTCTGCAAAAGAGGGCTTTAATGTTGAAGCAGCTTTCCATTGTATAGCCAAGAATGCATTGGAGAACGAGCCTGAAGAGGAGATATATCTGGACACATTTGATGTCAA
TGTTGGGAGACCACAAAGAGCATCAGGATACGATTGTTAGTCACCAGGGGATCATTCAAGACTTTGATGCTGCAAATAATAGTTTACTTGCATGAAATTCAGAGAAATAAAGTATACAGTCTCAATTTGTCCCTGGATCAAATGGAAGCACAGTCAACAGGAGCCCAGATGAAATTGCGTCTCATGTTTAAGCTGTTCAA
AAAATGGAAGGATGAGGTGCACTGAAATTAGCATTGGGAATTCGTTGGGATCATAAAATGCGTGAAACATGTTTATGAATCCGATTCTAATTCTTATTCCTAGGCGATAACAGTTTGAGGAAAGTGAGAATTCAGATGCAGGCTGGTGTTCTCATTCAAATGGCT