NA ----> NA

Intron 1 GTACAATGGG ... TGAGAAGGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.28	45	109	94
A2	69.81	32	71	77
A3	65.15	42	81	76
A4	79.63	11	38	48
A5	73.77	8	23	22
A6	73.68	5	15	13
A7	73.33	28	81	73
A8	81.42	30	125	138
A9	78.26	10	34	38
A10	71.27	26	65	64

>TRINITY_DN44141_c3_g1_i7 from sample A6 / Example spliced isoform

TGGCCATGCTACTTCATTGTTGGGCATTTGTTTGATTGCTTTCCCTCTCGTTTGTTTGTATTATTCTTGTTCAAAGCTCGTTTGTGGTTCAATGGGTGGTGAACTTAAGGCTAGTGCGAATGGACCATTGGAGATAATGTAGATTCCACAAATTTAGTGGGCAAAGGTTCAGTGGGTAGGGTATTTAAGGAAGGTCCTAA
CAGCTCATATTATTTTTTAGCATGGCCATGCTACTTTGTTGTTTGGTATTTGTTTTATTGGTTGCCTTCTAGTGTGTTTGTCTTATTGTTGTTCAAAGCTATTTTGTGGTTCAATGGGTGGTGAACTTAAGGCTAGCGCGGATGGCCCATTGTGAGTTTAGGAATGATGTAGATTGCACACATTGCAATAGGTGAAAGGG
AAGGCCAAGGAAGATGAGGTATGCATGGATCATGTGCAAAGATTGAGACCAAGGGTGAATTCTACATTTGTTGATAACAATCCATTTGGTCGGTTTAGGCTTGATACATATTTACAGGTGTGAAGGTTGAGGCAGATGGTGAGTTCTACATTTGTTGATAACAACCCATATTGCGGGTTTAGAGCTGATGCATCTTTACA
GGTACAATGGGCTAGGAGAAGGCTA

>TRINITY_DN44141_c3_g1_i11 from sample A6 / Example unspliced isoform:

TTTTTTAGCATGGCCATGCTACTTTGTTGTTTGGTATTTGTTTTATTGGTTGCCTTCTAGTGTGTTTGTCTTATTGTTGTTCAAAGCTATTTTGTGGTTCAATGGGTGGTGAACTTAAGGCTAGCGCGGATGGCCCATTGGAGCTGATGCAAATTCCACAAAGCTGGTGGGCAAAGGTGAGGGAGGCTCAGTTTGTGGGG
TAATTAAGGTATTAACAACCCATATTGTGAGTTTAGGAATGATGTAGATTGCACACATTGCAATAGGTGAAAGGGAAGGCCAAGGAAGATGAGGTGCAAAGATTGAGACCAAGGGTGAATTCTACATTTGTTGATAACAATCCATTTGGTCGGTTTAGGCTTGATACATATTTACAGgtacaatgggcaatgagaaggag


GTGTGAAGGTTGAGGCAGATGGTGAGTTCTACATTTGTTGATAACAACCCATATTGCGGGTTTAGAGCTGATGCATCTTTACAGGTACAATGGGCTAGGAGAAGGCTAAGGTGTAGGAGGTAAAAGGTGTATGATTGTTAATGAAGCCATTGAAATAGATGAAGCCATTGAAATGTCTATGGGAGAGATATGGGTTAGCT
GGTGAGTAAACATAGAATCATACGCCGGAAATGCATTTACTCGACAGTTGAGTGGCAGTTTCATTCAACAGATGTGACCTTTATTGACCACAGGGTGGATGAACATGTGAGTCTGCAGATTATATTGGAAAAGCATCTGGCAATTGGTTCTGGGAATCCAACAACACGGAATAAATTAAGACATTTTTGTAAAGAATCCA
ACAACACGGAATAAATTAAG

Intron 2 GTATGTTTAC ... GTCTTTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.55	16.3	45.2	36.3
A2	84.11	2.7	12.2	14.7
A3	88.53	3	25	21.3
A4	80.7	4.7	15	23.3
A5	98.33	0.2	7	11
A6	69.23	2	5	4
A7	60.37	4.2	3	9.7
A8	80.94	5.5	21	25.3
A9	80.3	1	4.2	4
A10	56.67	1.3	2	1.3

>TRINITY_DN134250_c1_g1_i11 from sample A1 / Example spliced isoform

GTATGTATAAGGGAATTTACTCATTTGTTGTTACATAATATTATGGTTCTATGAAATTTTAATTTAACTCATTTACTGCAGTATAATTATATCGTGTGTGTTTGTTTGAATTTAAAAGCAATTGAGGTATAAAAAGGTTCAATAGGTGGGGTAATTAAGGATGGTCCTAACAGACCATATTGTGAGTTTAAGAATGATGT
AAAGGAGGTATGGATGGTTCATGTGAGTTTGTTTAAATTAAGGTCATATTAAATTATTTGTTATTTATTTATTTATTTGAGTGTATGGGCATAAAGGAAGGTGGAAGCGCGCCAATGACATTGTTGGAATATCCTTTTATGCAAGTGAAAAGGTTGAAACTGAAGGTGAAGTCTACATTTTTTTATAACAATTCATTTTG
TGAGTATAGGGTTGATGCATATTTACAGGTACAATGGGCAATGAGAAGG

>TRINITY_DN134250_c1_g1_i9 from sample A1 / Example unspliced isoform:

GTATGTATAAGGGAATTTACTCATTTGTTGTTACATAATATTATGGTTCTATGAAATTTTAATTTAACTCATTTACTGCAGTATAATTATATCGTGTGTGTTTGTTTGAATTTAAAAGCAATTGAGGTATAAAAAGgtatgtttacataacattactcataatatatatcagtatttgtttaatcattctcatgagatat
ttggtttcttttaaatatttatgataatatttgatggtctttatttactattttttggtttgtctttttagGTTCAATAGGTGGGGTAATTAAGGATGGTCCTAACAGACCATATTGTGAGTTTAAGAATGATGTAAAGGAGGTATGGATGGTTCATGTGAGTTTGTTTAAATTAAGGTCATATTAAATTATTTGTTATT
TATTTATTTATTTGAGTGTATGGGCATAAAGGAAGGTGGAAGCGCGCCAATGACATTGTTGGAATATCCTTTTATGCAAGTGAAAAGGTTGAAACTGAAGAGGCAATGTAGTGCAAGTAAAGGAAGGTGGATGACTATTGATGAAAATGCTAAAATGTCTTCGTGTAAGGCAGACATGGGATATCAGGTTTAGGGTTGAA
GCATCTTTACAAGTACAATGGGTGAGGAGAAGGCTAAAGAGTAGGAGGTGCAGCTAGTGAAGGTGAAGTCGGAGGTGGTTGTCCTTGGATAGCCATGGAA

Intron 3 GTATGGATGG ... GGGCATAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.92	47.6	11.6	23.5
A2	40.97	31	16.9	26.2
A3	52.27	19.9	21.3	22.4
A4	31.43	36.1	15.4	17.8
A5	27.89	17.6	6.2	7.9
A6	12.08	3.9	0.6	0.5
A7	15.29	27.9	6.3	3.8
A8	12.02	39.4	6.8	4.3
A9	30.4	11.9	4.6	5.9
A10	32.16	14	5	8.3

>TRINITY_DN134250_c1_g1_i7 from sample A1 / Example spliced isoform

GTATGTATAAGGGAATTTACTCATTTGTTGTTACATAATATTATGGTTCTATGAAATTTTAATTTAACTCATTTACTGCAGTATAATTATATCGTGTGTGTTTGTTTGAATTTAAAAGCAATTGAGGTATAAAAAGGTATGTTTACATAACATTACTCATAATATATATCAGTATTTGTTTAATCATTCTCATGAGATAT
TTGGTTTCTTTTAAATATTTATGATAATATTTGATGGTCTTTATTTACTATTTTTTGGTTTGTCTTTTTAGGTTCAATAGGTGGGGTAATTAAGGATGGTCCTAACAGACCATATTGTGAGTTTAAGAATGATGTAAAGGAGGAAGGTGGAAGCGCGCCAATGACATTGTTGGAATATCCTTTTATGCAAGTGAAAAGGT
TGAAACTGAAGAGGCAATGTAGTGCAAGTAAAGGAAGGTGGATGACTATTGATGAAAATGCTAAAATGTCTTCGTGTAAGGCAGACATGGGATATCAGGTTTAGGGTTGAAGCATCTTTACAAGTACAATGGGTGAGGAGAAGGCTAAAGAGTAGGAGGTGCAGCTAGTGAAGGTGAAGTCGGAGGTGGTTGTCCTTGGA
TAGCCATGGAA

>TRINITY_DN134250_c1_g1_i3 from sample A1 / Example unspliced isoform:

GTATGTATAAGGGAATTTACTCATTTGTTGTTACATAATATTATGGTTCTATGAAATTTTAATTTAACTCATTTACTGCAGTATAATTATATCGTGTGTGTTTGTTTGAATTTAAAAGCAATTGAGGTATAAAAAGGTTCAATAGGTGGGGTAATTAAGGATGGTCCTAACAGACCATATTGTGAGTTTAAGAATGATGT
AAAGGAGgtatggatggttcatgtgagtttgtttaaattaaggtcatattaaattatttgttatttatttatttatttgagtgtatgggcataaagGAAGGTGGAAGCGCGCCAATGACATTGTTGGAATATCCTTTTATGCAAGTGAAAAGGTTGAAACTGAAGAGGCAATGTAGTGCAAGTAAAGGAAGGTGGATGAC
TATTGATGAAAATGCTAAAATGTCTTCGTGTAAGGCAGACATGGGATATCAGGTTTAGGGTTGAAGCATCTTTACAAGTACAATGGGTGAGGAGAAGGCTAAAGAGTAGGAGGTGCAGCTAGTGAAGGTGAAGTCGGAGGTGGTTGTCCTTGGATAGCCATGGAA

Intron 4 GTATGCATGG ... TGGTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.65	34.5	118.5	47.5
A2	71.21	15.5	62.5	12
A3	72.58	22.5	60	43
A4	59.7	30	58	29.5
A5	62.35	9	18.5	10
A6	75	3	4.5	3.5
A7	47.51	23.5	23	17
A8	72.31	10	18.5	23.5
A9	84.62	2	7	9
A10	72.5	5	8.5	11

>TRINITY_DN120820_c1_g1_i4 from sample A3 / Example spliced isoform

CCCATATTGCGGGTTGATACACATCTACAAGTACAATGGGCAATGAGAAGGCTAAAGTGTAGGAGGTAATGGAAGGTGGATCACTATCGATGAAAATGTTGAAATGTCTTTGTGTGAGAGAGATATGGGGTAGTCAGTAAACAAGGATGATAAGGTTGTCCTTGGATAGCCATGGAATGAAGGAAGCATTCAAGAGTTGT
AGAGTGGAGTCTTTGTTTGTTATATCTCTTTCTTTGTTTTTTTGTTTT

>TRINITY_DN120820_c1_g1_i6 from sample A3 / Example unspliced isoform:

CCCATATTGCGGGTTGATACACATCTACAAGTACAATGGGCAATGAGAAGGCTAAAGTGTAGGAGGTAATGGAAGGTGGATCACTATCGATGAAAATGTTGAAATGTCTTTGTGTGAGAGAGATATGGGGTAGTCAGTAAACAAGGATGATAAGgtatgcatggtttcaattgtttttgtttaaattagattcattgtat
tatttgtcatttctttatcaatttggtttgtgtttaaaatttataatacgattttttggtatctatttaatgcttggcaaaaatttggtgtgcagGTTGTCCTTGGATAGCCATGGAATGAAGGAAGCATTCAAGAGTTGTAGAGTGGAGTCTTTGTTTGTTATATCTCTTTCTTTGTTTTTTTGTTTT

Intron 5 GTATGCATGG ... ATTTGTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.72	43	155	145
A2	75.29	32	90	105
A3	69.28	45	80	123
A4	66.54	43	95	76
A5	75.24	13	45	34
A6	65.52	5	9	10
A7	67.16	11	31	14
A8	64.29	10	17	19
A9	73.33	2	5	6
A10	55.56	4	3	7

>TRINITY_DN45216_c2_g3_i7 from sample A8 / Example spliced isoform

CTAAGGAGTAGGAGGTATGCATTATTTTATGATAGCTGAACCTAACCCATTTTGTGTTAGATCATTATATCATGTTTTTGTGGGTTTAGGGTTGATGTGGACCGTACAAAGGGCAATGAGAAGGCTAAGGAGTAGGAGGTAGGCATTGTTCGATGATAGTTGAACCTGACAATTTTATGATAGCTGAACCTAACCCATTT
TTTGTTAGATCATTATATCATGTTTTTGTGGGTTTAGGGTTGATGCATATTTGTGGGTACAAAGGGCAATGAGAAGGATAAGGAGTAGGAGGTATGCATTGTTCTATGGTAGTTGAACCTAACCTATTCTTTGTTACATCATTATATTATGTGTTTGTTTAAATTTAAAGGCAATGTAGTGCAAGTAATGGAAAGTGGAT
GGTTGTTGATGAGAATGCTGAAATGTCTTTGTGTGAGAGAGATATGGGGTATTCAGTAAACAAGGGCGATAAGGTAGAGGCAGATGGTGAGTTATACATTTGTTGATATCAACCCATATTGTGGGTTTAGGGTTGATGCATCTTTTCAGGTACAGTGGGTGAAGAAAGGATACGAAGTAGGAGGA

>TRINITY_DN45216_c2_g3_i6 from sample A8 / Example unspliced isoform:

CTAAGGAGTAGGAGGTATGCATTATTTTATGATAGCTGAACCTAACCCATTTTGTGTTAGATCATTATATCATGTTTTTGTGGGTTTAGGGTTGATGTGGACCGTACAAAGGGCAATGAGAAGGCTAAGGAGTAGGAGGTAGGCATTGTTCGATGATAGTTGAACCTGACAATTTTATGATAGCTGAACCTAACCCATTT
TTTGTTAGATCATTATATCATGTTTTTGTGGGTTTAGGGTTGATGCATATTTGTGGGTACAAAGGGCAATGAGAAGGATAAGGAGTAGGAGGTATGCATTGTTCTATGGTAGTTGAACCTAACCTATTCTTTGTTACATCATTATATTATGTGTTTGTTTAAATTTAAAGGCAATGTAGTGCAAGTAATGGAAAGTGGAT
GGTTGTTGATGAGAATGCTGAAATGTCTTTGTGTGAGAGAGATATGGGGTATTCAGTAAACAAGGGCGATAAGgtatgcatggtttcaattgtttttgtttaaattagattcattgtattatttgtcatttatttatatattgtgtgcaaaggggtaaaggaaggtataggatctctaatgacattgctgaaatatcttt
gtgtgcatttgtgaagGTAGAGGCAGATGGTGAGTTATACATTTGTTGATATCAACCCATATTGTGGGTTTAGGGTTGATGCATCTTTTCAGGTACAGTGGGTGAAGAAAGGATACGAAGTAGGAGGA