sp|A2RVM8|HIP37_ARATH ----> Heavy metal-associated isoprenylated plant protein 37

Intron 1 GTATTCTTCC ... CTGCTCATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.03	52	24	25
A2	31.71	42	19	20
A3	40	51	39	29
A4	22.22	28	4	12
A5	17.86	23	7	3
A6	37.29	37	23	21
A7	30.25	83	34	38
A8	35.29	44	20	28
A9	30.88	75	33	34
A10	31.14	94	33	52

>TRINITY_DN111794_c0_g1_i6 from sample A1 / Example spliced isoform

AATTAAATGTGTAAGTTGGGGACGTGATTGGGATGGGAGCTTTGATGGGAATTAAATGTGTAAGTTGGTTGACAGCTATGACACATGTGATTTCATGCTTTGGGTCCCTGAAAGAATTATTACTATTCCCTTTCAATATAGTGACAAGACAAGAGAAACAAGAAAAAAGATAACCAGCAGAGTGCTGCCTTTCTCTCTGC
ACATTTTTTTCTTACTGAGCGATCGTGTGGCCTTTCCTCTGACTGAGCTGGCTGAGAGGGCTTGTACGAACAGTGGTTACCCTGTTGAGCTTTTCATTTTGCTCATCACGCGCCGTTGAAAAGGAGTTGGGCGGCTGCTGACCGACCGCTGTATCTCACCAGAAAGATCAAATAACCGAACTGCAGAGGGTTTAATTGGC
TGCCATTGAGTGGGAGTTTCTTGCTCCCTCACAAGGCACGAGGTAGAATTAATTCCTAGTCAAATTTGGACTCACGGAGTCCCGGAGCAGGCATGACAAAGGACGAAGATTTCAAGCTTATTAAAACCCAGACTTGGGTTCTAAAGGTTTATATACACTGCGAGGGATGCAAGAAAAAGGTCAAGAAAGTTCTGCAGAGC
ATCGATGGTGTGTTCACTACCTCCATTGATGCTGCCGAGCAGAAGGTGACTGTTACAGGAAATGTGGCTTCCGCTGATATCTTGTTGAAGAAGTTGCTGAAATCCGGGAAGCATGCGGAGTTGTGGCCGGTGGCTCAGAACAAACAGCAGAATAACGAGCCAAAGATTCCGAAGCAACAGAAAGAGCAGAAAGCGGGCAA
TGATGGCGGTGGTGATGTGAATGAATATGCAGCGGCGCCGAAAGGGGAGAAAGATAAGGGAGATGGCGAGAAATCTGGTGGTGGTGGGAACGAAGGAGGCGGTGGTGGAAAGAAGCGTAACGGCAAGAAAGGCGGCGGTGGCGGTGGTGGTGGAAATGGAGGTGGAGAAGTGGGACGAGACGAAAATAGTGGCGGTGATG
GCGAGGGTAAGAAAGGCGGAGATGGAGCTAAGCAGAGTAGTGGCGGGGACGGAGGAGCGGCAGAAGGCGGTGGCGGCGATGCTAGTGGAGGCAGTGGAGGAAAGAAGAAGAAGGGCGGAAAAGGTGGAGATGGAATGACCGGCGAGTCTACTAGCTACGAAGAAACTGAATTAGCAGCAGCAACAGCAGCAGCAGGGGCA
GGATTCGATCCTATGATGGGCGGGGTAGGGTACTGGGAAAACTCTATGCAGAGAACGGGTGTAGTACACAACCGGCAGATGTTGCAGTATAGACAACACTATCCTCCCCCTATGTACGCTCCCACTGTGTATGCTAACCCTGCAAATCAAGCGGAGTATGCCACCATGTTCAGTGACGAAAATACTGGCGGCTGCTCGAT
TATGTAACTAAATCTCCATCAGTTCATTTGCAGGTTTCGTTTAGAGGAGTAGGACTGGAGGTTATCAGGGCATGCATGACGCAGTGACCATAGAATGGTTTTGAAACACCGTAGCCAGATTTAGCTGCTCGTAAATCTGCTAAAAGTAGCGGCGTACTTGCTTAGAGAGAAACGGAGAGGAGTTGTTGTGTGTTGTAATA
AATGTGGTCCGTCAAACTCTAAATTTGCGTAGGTAGGGGAACAGACAAGGGCTAGTACGTTGGCCTCTTTTTATTTCCTGTATGTAATTATGTGAGTACTTGCAATAAGACTAAGACTGCTTTGTATACCTATAATGTCAGATGGGAATCGAATATGAGATACACTGGCTCTCTGAATATTGCAATGGCATGGCAGGACT
GGATTCACTGTATTGCACTCTGTATATTCAGTGTGTACCATATCAAATCATTCCTTTTATTTTTCCCAAACTTCACC

>TRINITY_DN111794_c0_g1_i3 from sample A1 / Example unspliced isoform:

AATTAAATGTGTAAGTTGGGGACGTGATTGGGATGGGAGCTTTGATGGGAATTAAATGTGTAAGTTGGTTGACAGCTATGACACATGTGATTTCATGCTTTGGGTCCCTGAAAGAATTATTACTATTCCCTTTCAATATAGTGACAAGACAAGAGAAACAAGAAAAAAGATAACCAGCAGAGTGCTGCCTTTCTCTCTGC
ACATTTTTTTCTTACTGAGCGATCGTGTGGCCTTTCCTCTGACTGAGCTGGCTGAGAGGGCTTGTACGAACAGTGGTTACCCTGTTGAGgtattcttcctttaattataaagcacccacttactttttctgtaggccgtgtaagctgctttttatggtaacagactggtctctgctcatagCTTTTCATTTTGCTCATCA
CGCGCCGTTGAAAAGGAGTTGGGCGGCTGCTGACCGACCGCTGTATCTCACCAGAAAGATCAAATAACCGAACTGCAGAGGGTTTAATTGGCTGCCATTGAGTGGGAGTTTCTTGCTCCCTCACAAGGCACGAGGTAGAATTAATTCCTAGTCAAATTTGGACTCACGGAGTCCCGGAGCAGGCATGACAAAGGACGAAG
ATTTCAAGCTTATTAAAACCCAGACTTGGGTTCTAAAGGTTTATATACACTGCGAGGGATGCAAGAAAAAGGTCAAGAAAGTTCTGCAGAGCATCGATGGTGTGTTCACTACCTCCATTGATGCTGCCGAGCAGAAGGTGACTGTTACAGGAAATGTGGCTTCCGCTGATATCTTGTTGAAGAAGTTGCTGAAATCCGGG
AAGCATGCGGAGTTGTGGCCGGTGGCTCAGAACAAACAGCAGAATAACGAGCCAAAGATTCCGAAGCAACAGAAAGAGCAGAAAGCGGGCAATGATGGCGGTGGTGATGTGAATGAATATGCAGCGGCGCCGAAAGGGGAGAAAGATAAGGGAGATGGCGAGAAATCTGGTGGTGGTGGGAACGAAGGAGGCGGTGGTGG
AAAGAAGCGTAACGGCAAGAAAGGCGGCGGTGGCGGTGGTGGTGGAAATGGAGGTGGAGAAGTGGGACGAGACGAAAATAGTGGCGGTGATGGCGAGGGTAAGAAAGGCGGAGATGGAGCTAAGCAGAGTAGTGGCGGGGACGGAGGAGCGGCAGAAGGCGGTGGCGGCGATGCTAGTGGAGGCAGTGGAGGAAAGAAGA
AGAAGGGCGGAAAAGGTGGAGATGGAATGACCGGCGAGTCTACTAGCTACGAAGAAACTGAATTAGCAGCAGCAACAGCAGCAGCAGGGGCAGGATTCGATCCTATGATGGGCGGGGTAGGGTACTGGGAAAACTCTATGCAGAGAACGGGTGTAGTACACAACCGGCAGATGTTGCAGTATAGACAACACTATCCTCCC
CCTATGTACGCTCCCACTGTGTATGCTAACCCTGCAAATCAAGCGGAGTATGCCACCATGTTCAGTGACGAAAATACTGGCGGCTGCTCGATTATGTAACTAAATCTCCATCAGTTCATTTGCAGGTTTCGTTTAGAGGAGTAGGACTGGAGGTTATCAGGGCATGCATGACGCAGTGACCATAGAATGGTTTTGAAACA
CCGTAGCCAGATTTAGCTGCTCGTAAATCTGCTAAAAGTAGCGGCGTACTTGCTTAGAGAGAAACGGAGAGGAGTTGTTGTGTGTTGTAATAAATGTGGTCCGTCAAACTCTAAATTTGCGTAGGTAGGGGAACAGACAAGGGCTAGTACGTTGGCCTCTTTTTATTTCCTGTATGTAATTATGTGAGTACTTGCAATAA
GACTAAGACTGCTTTGTATACCTATAATGTCAGATGGGAATCGAATATGAGATACACTGGCTCTCTGAATATTGCAATGGCATGGCAGGACTGGATTCACTGTATTGCACTCTGTATATTCAGTGTGTACCATATCAAATCATTCCTTTTATTTTTCCCAAACTTCACC

Intron 2 GTAAAGGTGG ... GGAGGTGGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	36.36	14	9	7
A2	39.53	13	14	3
A3	49.3	18	21	14
A4	46.34	11	15	4
A5	33.33	3	3	0
A6	47.22	38	45	23
A7	47.54	80	97	48
A8	49.06	68	81	50
A9	55	36	47	41
A10	46.96	61	72	36

>TRINITY_DN37883_c0_g1_i3 from sample A6 / Example spliced isoform

TAAATGTGTAAGTTGGTTGACAGCTATGACACATGTGATTTCATGCTTTGGGTCCCTGAAAGAATTATTACTATTCCCTTTCAATATAGTGACAAGACAAGAGAAACAAGAAAAAAGATAACCAGCAGAGTGCTGCCTTTCTCTCTGCACATTTTTTTCTTACTGAGCGATCGTGTGGCCTTTCCTCTGACTGAGCTGGC
TGAGAGGGCTTGTACGAACAGTGGTTACCCTGTTGAGGTATTCTTCCTTTAATTATAAAGCACCCACTTACTTTTTCTGTAGGCCGTGTAAGCTGCTTTTTATGGTAACAGACTGGTCTCTGCTCATAGCTTTTCATTTTGCTCATCACGCGCCGTTGAAAAGGAGTTGGGCGGCTGCTGACCGACCGCTGTATCTCACC
AGAAAGATCAAATAACCGAACTGCAGAGGGTTTAATTGGCTGCCATTGAGTGGGAGTTTCTTGCTCCCTCACAAGGCACGAGGTAGAATTAATTCCTAGTCAAATTTGGACTCACGGAGTCCCGGAGCAGGCATGACAAAGGACGAAGATTTCAAGCTTATTAAAACCCAGACTTGGGTTCTAAAGGTTTATATACACTG
CGAGGGATGCAAGAAAAAGGTCAAGAAAGTTCTGCAGAGCATCGATGGTGTGTTCACTACCTCCATTGATGCTGCCGAGCAGAAGGTGACTGTTACAGGAAATGTGGCTTCCGCTGATATCTTGTTGAAGAAGTTGCTGAAATCCGGGAAGCATGCGGAGTTGTGGCCGGTGGCTCAGAACAAACAGCAGAATAACGAGC
CAAAGATTCCGAAGCAACAGAAAGAGCAGAAAGCGGGCAATGATGGCGGTGGTGATGTGAATGAATATGCAGCGGCGCCGAAAGGGGAGAAAGATAAGGGAGATGGCGAGAAATCTGGTGGTGGTGGGAACGAAGGAGGCGGTGGTGGAAAGAAGCGTAACGGCAAGAAAGGCGGCGGTGGCGGTGGTGGTGGAAATGGA
GGTGGAGAAGTGGGACGAGACGAAAATAGTGGCGGTGATGGCGAGGGTAAGAAAGGCGGAGATGGAGCTAAGCAGAGTAGTGGCGGGGACGGAGGAGCGGCAGAAGGCGGTGGCGGCGATGCTAGTGGAGGCAGTGGAGGAAAGAAGAAGAAGGGCGGAAAAGGTGGAGATGGAATGATCGGCGAGTCTACTAGCTACGA
AGAAACTGAATTAGCAGCAGCAACAGCAGCAGCAGGGGCAGGATTCGATCCTATGATGGGCGGGGTAGGGTACTGGGAAAACTCTATGCAGAGAACGGGTGTAGTACACAACCGGCAGATGTTGCAGTATAGACAACACTATCCTCCCCCTATGTACGCTCCCACTGTGTATGCTAACCCTGCAAATCAAGCGGAGTATG
CCACCATGTTCAGTGACGAAAATACTGGCGGCTGCTCGATTATGTAACTAAATCTCCATCAGTTCATTTGCAGGTTTCGTTTAGAGGAGTAGGACTGGAGGTTATCAGGGCATGCATGACGCAGTGACCATAGAATGGTTTTGAAACACCGTAGCCAGATTTAGCTGCTCGTAAATCTGCTAAAAGTAGCGGCGTACTTG
CTTAGAGAGAAACGGAGAGGAGTTGTTGTGTGTTGTAATAAATGTGGTCCGTCAAACTCTAAATTTGCGTAGGTAGGGGAACAGACAAGGGCTAGTACGTTGGCCTCTTTTTATTTCCTGTATGTAATTATGTGAGTACTTGCAATAAGACTAAGACTGCTTTGTATACCTATAATGTCAGATGGGAATCGAATATGAGA
TACACTGGCTCTCTGAATATTGCAATGGCATGGCAGGACTGGATTCACTGTATTGCACTCTGTATATTCAGTGTGTACCATATCAAATCATTCCTTTTATTTTTCCCAAACTTCACCTACCCAAAAAAGTTAAAATAAGGTATATATACATACTCTAGTATATATTACATGTATTTTGAAAACTTATCTGGTTATTATAT
ATATTTTTGGACAGTTTAGCTTGGAAGTTTATCCATCATCATATAAATATACACACCC

>TRINITY_DN37883_c0_g1_i7 from sample A6 / Example unspliced isoform:

TAAATGTGTAAGTTGGTTGACAGCTATGACACATGTGATTTCATGCTTTGGGTCCCTGAAAGAATTATTACTATTCCCTTTCAATATAGTGACAAGACAAGAGAAACAAGAAAAAAGATAACCAGCAGAGTGCTGCCTTTCTCTCTGCACATTTTTTTCTTACTGAGCGATCGTGTGGCCTTTCCTCTGACTGAGCTGGC
TGAGAGGGCTTGTACGAACAGTGGTTACCCTGTTGAGCTTTTCATTTTGCTCATCACGCGCCGTTGAAAAGGAGTTGGGCGGCTGCTGACCGACCGCTGTATCTCACCAGAAAGATCAAATAACCGAACTGCAGAGGGTTTAATTGGCTGCCATTGAGTGGGAGTTTCTTGCTCCCTCACAAGGCACGAGGTAGAATTAA
TTCCTAGTCAAATTTGGACTCACGGAGTCCCGGAGCAGGCATGACAAAGGACGAAGATTTCAAGCTTATTAAAACCCAGACTTGGGTTCTAAAGGTTTATATACACTGCGAGGGATGCAAGAAAAAGGTCAAGAAAGTTCTGCAGAGCATCGATGGTGTGTTCACTACCTCCATTGATGCTGCCGAGCAGAAGGTGACTG
TTACAGGAAATGTGGCTTCCGCTGATATCTTGTTGAAGAAGTTGCTGAAATCCGGGAAGCATGCGGAGTTGTGGCCGGTGGCTCAGAACAAACAGCAGAATAACGAGCCAAAGATTCCGAAGCAACAGAAAGAGCAGAAAGCGGGCAATGATGGCGGTGGTGATGTGAATGAATATGCAGCGGCGCCGAAAGGGGAGAAA
GATAAGGGAGATGGCGAGAAATCTGGTGGTGGTGGGAACGAAGGAGGCGGTGGTGGAAAGAAGCGTAACGGCAAGAAAGGCGGCGGTGGCGGTGGTGGTGGAAATGGAGGTGGAGAAGTGGGACGAGACGAAAATAGTGGCGGTGATGGCGAGGGTAAGAAAGGCGGAGATGGAGCTAAGCAGAGTAGTGGCGGGGACGG
AGGAGCGGCAGAAGGCGGTGGCGGCGATGCTAGTGGAGGCAGTGGAGGAAAGAAGAAGAAGGGCGGAAAAGgtaaaggtggaggtggaggtggaggtggagGTGGAGATGGAATGATCGGCGAGTCTACTAGCTACGAAGAAACTGAATTAGCAGCAGCAACAGCAGCAGCAGGGGCAGGATTCGATCCTATGATGGGCG
GGGTAGGGTACTGGGAAAACTCTATGCAGAGAACGGGTGTAGTACACAACCGGCAGATGTTGCAGTATAGACAACACTATCCTCCCCCTATGTACGCTCCCACTGTGTATGCTAACCCTGCAAATCAAGCGGAGTATGCCACCATGTTCAGTGACGAAAATACTGGCGGCTGCTCGATTATGTAACTAAATCTCCATCAG
TTCATTTGCAGGTTTCGTTTAGAGGAGTAGGACTGGAGGTTATCAGGGCATGCATGACGCAGTGACCATAGAATGGTTTTGAAACACCGTAGCCAGATTTAGCTGCTCGTAAATCTGCTAAAAGTAGCGGCGTACTTGCTTAGAGAGAAACGGAGAGGAGTTGTTGTGTGTTGTAATAAATGTGGTCCGTCAAACTCTAA
ATTTGCGTAGGTAGGGGAACAGACAAGGGCTAGTACGTTGGCCTCTTTTTATTTCCTGTATGTAATTATGTGAGTACTTGCAATAAGACTAAGACTGCTTTGTATACCTATAATGTCAGATGGGAATCGAATATGAGATACACTGGCTCTCTGAATATTGCAATGGCATGGCAGGACTGGATTCACTGTATTGCACTCTG
TATATTCAGTGTGTACCATATCAAATCATTCCTTTTATTTTTCCCAAACTTCACCTACCCAAAAAAGTTAAAATAAGGTATATATACATACTCTAGTATATATTACATGTATTTTGAAAACTTATCTGGTTATTATATATATTTTTGGACAGTTTAGCTTGGAAGTTTATCCATCATCATATAAATATACACACCC

Intron 3 GTATTCTTCC ... CGTTGAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	36.71	50	24	34
A2	43.69	29	19	26
A3	47.56	43	39	39
A4	33.33	28	4	24
A5	22.86	27	7	9
A6	41.94	36	23	29
A7	39.82	68	34	56
A8	36.9	53	20	42
A9	32.84	90	33	55
A10	41	77	33	74

>TRINITY_DN111794_c0_g1_i2 from sample A1 / Example spliced isoform

AATTAAATGTGTAAGTTGGGGACGTGATTGGGATGGGAGCTTTGATGGGAATTAAATGTGTAAGTTGGTTGACAGCTATGACACATGTGATTTCATGCTTTGGGTCCCTGAAAGAATTATTACTATTCCCTTTCAATATAGTGACAAGACAAGAGAAACAAGAAAAAAGATAACCAGCAGAGTGCTGCCTTTCTCTCTGC
ACATTTTTTTCTTACTGAGCGATCGTGTGGCCTTTCCTCTGACTGAGCTGGCTGAGAGGGCTTGTACGAACAGTGGTTACCCTGTTGAGGAGTTGGGCGGCTGCTGACCGACCGCTGTATCTCACCAGAAAGATCAAATAACCGAACTGCAGAGGGTTTAATTGGCTGCCATTGAGTGGGAGTTTCTTGCTCCCTCACAA
GGCACGAGGTAGAATTAATTCCTAGTCAAATTTGGACTCACGGAGTCCCGGAGCAGGCATGACAAAGGACGAAGATTTCAAGCTTATTAAAACCCAGACTTGGGTTCTAAAGGTTTATATACACTGCGAGGGATGCAAGAAAAAGGTCAAGAAAGTTCTGCAGAGCATCGATGGTGTGTTCACTACCTCCATTGATGCTG
CCGAGCAGAAGGTGACTGTTACAGGAAATGTGGCTTCCGCTGATATCTTGTTGAAGAAGTTGCTGAAATCCGGGAAGCATGCGGAGTTGTGGCCGGTGGCTCAGAACAAACAGCAGAATAACGAGCCAAAGATTCCGAAGCAACAGAAAGAGCAGAAAGCGGGCAATGATGGCGGTGGTGATGTGAATGAATATGCAGCG
GCGCCGAAAGGGGAGAAAGATAAGGGAGATGGCGAGAAATCTGGTGGTGGTGGGAACGAAGGAGGCGGTGGTGGAAAGAAGCGTAACGGCAAGAAAGGCGGCGGTGGCGGTGGTGGTGGAAATGGAGGTGGAGAAGTGGGACGAGACGAAAATAGTGGCGGTGATGGCGAGGGTAAGAAAGGCGGAGATGGAGCTAAGCA
GAGTAGTGGCGGGGACGGAGGAGCGGCAGAAGGCGGTGGCGGCGATGCTAGTGGAGGCAGTGGAGGAAAGAAGAAGAAGGGCGGAAAAGGTGGAGATGGAATGACCGGCGAGTCTACTAGCTACGAAGAAACTGAATTAGCAGCAGCAACAGCAGCAGCAGGGGCAGGATTCGATCCTATGATGGGCGGGGTAGGGTACT
GGGAAAACTCTATGCAGAGAACGGGTGTAGTACACAACCGGCAGATGTTGCAGTATAGACAACACTATCCTCCCCCTATGTACGCTCCCACTGTGTATGCTAACCCTGCAAATCAAGCGGAGTATGCCACCATGTTCAGTGACGAAAATACTGGCGGCTGCTCGATTATGTAACTAAATCTCCATCAGTTCATTTGCAGG
TTTCGTTTAGAGGAGTAGGACTGGAGGTTATCAGGGCATGCATGACGCAGTGACCATAGAATGGTTTTGAAACACCGTAGCCAGATTTAGCTGCTCGTAAATCTGCTAAAAGTAGCGGCGTACTTGCTTAGAGAGAAACGGAGAGGAGTTGTTGTGTGTTGTAATAAATGTGGTCCGTCAAACTCTAAATTTGCGTAGGT
AGGGGAACAGACAAGGGCTAGTACGTTGGCCTCTTTTTATTTCCTGTATGTAATTATGTGAGTACTTGCAATAAGACTAAGACTGCTTTGTATACCTATAATGTCAGATGGGAATCGAATATGAGATACACTGGCTCTCTGAATATTGCAATGGCATGGCAGGACTGGATTCACTGTATTGCACTCTGTATATTCAGTGT
GTACCATATCAAATCATTCCTTTTATTTTTCCCAAACTTCACC

>TRINITY_DN111794_c0_g1_i3 from sample A1 / Example unspliced isoform:

AATTAAATGTGTAAGTTGGGGACGTGATTGGGATGGGAGCTTTGATGGGAATTAAATGTGTAAGTTGGTTGACAGCTATGACACATGTGATTTCATGCTTTGGGTCCCTGAAAGAATTATTACTATTCCCTTTCAATATAGTGACAAGACAAGAGAAACAAGAAAAAAGATAACCAGCAGAGTGCTGCCTTTCTCTCTGC
ACATTTTTTTCTTACTGAGCGATCGTGTGGCCTTTCCTCTGACTGAGCTGGCTGAGAGGGCTTGTACGAACAGTGGTTACCCTGTTGAGgtattcttcctttaattataaagcacccacttactttttctgtaggccgtgtaagctgctttttatggtaacagactggtctctgctcatagcttttcattttgctcatca
cgcgccgttgaaaagGAGTTGGGCGGCTGCTGACCGACCGCTGTATCTCACCAGAAAGATCAAATAACCGAACTGCAGAGGGTTTAATTGGCTGCCATTGAGTGGGAGTTTCTTGCTCCCTCACAAGGCACGAGGTAGAATTAATTCCTAGTCAAATTTGGACTCACGGAGTCCCGGAGCAGGCATGACAAAGGACGAAG
ATTTCAAGCTTATTAAAACCCAGACTTGGGTTCTAAAGGTTTATATACACTGCGAGGGATGCAAGAAAAAGGTCAAGAAAGTTCTGCAGAGCATCGATGGTGTGTTCACTACCTCCATTGATGCTGCCGAGCAGAAGGTGACTGTTACAGGAAATGTGGCTTCCGCTGATATCTTGTTGAAGAAGTTGCTGAAATCCGGG
AAGCATGCGGAGTTGTGGCCGGTGGCTCAGAACAAACAGCAGAATAACGAGCCAAAGATTCCGAAGCAACAGAAAGAGCAGAAAGCGGGCAATGATGGCGGTGGTGATGTGAATGAATATGCAGCGGCGCCGAAAGGGGAGAAAGATAAGGGAGATGGCGAGAAATCTGGTGGTGGTGGGAACGAAGGAGGCGGTGGTGG
AAAGAAGCGTAACGGCAAGAAAGGCGGCGGTGGCGGTGGTGGTGGAAATGGAGGTGGAGAAGTGGGACGAGACGAAAATAGTGGCGGTGATGGCGAGGGTAAGAAAGGCGGAGATGGAGCTAAGCAGAGTAGTGGCGGGGACGGAGGAGCGGCAGAAGGCGGTGGCGGCGATGCTAGTGGAGGCAGTGGAGGAAAGAAGA
AGAAGGGCGGAAAAGGTGGAGATGGAATGACCGGCGAGTCTACTAGCTACGAAGAAACTGAATTAGCAGCAGCAACAGCAGCAGCAGGGGCAGGATTCGATCCTATGATGGGCGGGGTAGGGTACTGGGAAAACTCTATGCAGAGAACGGGTGTAGTACACAACCGGCAGATGTTGCAGTATAGACAACACTATCCTCCC
CCTATGTACGCTCCCACTGTGTATGCTAACCCTGCAAATCAAGCGGAGTATGCCACCATGTTCAGTGACGAAAATACTGGCGGCTGCTCGATTATGTAACTAAATCTCCATCAGTTCATTTGCAGGTTTCGTTTAGAGGAGTAGGACTGGAGGTTATCAGGGCATGCATGACGCAGTGACCATAGAATGGTTTTGAAACA
CCGTAGCCAGATTTAGCTGCTCGTAAATCTGCTAAAAGTAGCGGCGTACTTGCTTAGAGAGAAACGGAGAGGAGTTGTTGTGTGTTGTAATAAATGTGGTCCGTCAAACTCTAAATTTGCGTAGGTAGGGGAACAGACAAGGGCTAGTACGTTGGCCTCTTTTTATTTCCTGTATGTAATTATGTGAGTACTTGCAATAA
GACTAAGACTGCTTTGTATACCTATAATGTCAGATGGGAATCGAATATGAGATACACTGGCTCTCTGAATATTGCAATGGCATGGCAGGACTGGATTCACTGTATTGCACTCTGTATATTCAGTGTGTACCATATCAAATCATTCCTTTTATTTTTCCCAAACTTCACC

Intron 4 GTAAGAAGCG ... TGTGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.07	132	13.9	30.1
A2	23.95	49	5.3	16.3
A3	22.91	74	11.9	23.1
A4	20.17	52	3.4	13.7
A5	30.44	29	1.7	13.7
A6	25.27	130	17.1	46.4
A7	22.76	332	41	102.9
A8	22.54	208	21.1	63.1
A9	22.82	233	17	71.9
A10	25.05	314	19.4	110.4

>TRINITY_DN111794_c0_g1_i3 from sample A1 / Example spliced isoform

AATTAAATGTGTAAGTTGGGGACGTGATTGGGATGGGAGCTTTGATGGGAATTAAATGTGTAAGTTGGTTGACAGCTATGACACATGTGATTTCATGCTTTGGGTCCCTGAAAGAATTATTACTATTCCCTTTCAATATAGTGACAAGACAAGAGAAACAAGAAAAAAGATAACCAGCAGAGTGCTGCCTTTCTCTCTGC
ACATTTTTTTCTTACTGAGCGATCGTGTGGCCTTTCCTCTGACTGAGCTGGCTGAGAGGGCTTGTACGAACAGTGGTTACCCTGTTGAGGTATTCTTCCTTTAATTATAAAGCACCCACTTACTTTTTCTGTAGGCCGTGTAAGCTGCTTTTTATGGTAACAGACTGGTCTCTGCTCATAGCTTTTCATTTTGCTCATCA
CGCGCCGTTGAAAAGGAGTTGGGCGGCTGCTGACCGACCGCTGTATCTCACCAGAAAGATCAAATAACCGAACTGCAGAGGGTTTAATTGGCTGCCATTGAGTGGGAGTTTCTTGCTCCCTCACAAGGCACGAGGTAGAATTAATTCCTAGTCAAATTTGGACTCACGGAGTCCCGGAGCAGGCATGACAAAGGACGAAG
ATTTCAAGCTTATTAAAACCCAGACTTGGGTTCTAAAGGTTTATATACACTGCGAGGGATGCAAGAAAAAGGTCAAGAAAGTTCTGCAGAGCATCGATGGTGTGTTCACTACCTCCATTGATGCTGCCGAGCAGAAGGTGACTGTTACAGGAAATGTGGCTTCCGCTGATATCTTGTTGAAGAAGTTGCTGAAATCCGGG
AAGCATGCGGAGTTGTGGCCGGTGGCTCAGAACAAACAGCAGAATAACGAGCCAAAGATTCCGAAGCAACAGAAAGAGCAGAAAGCGGGCAATGATGGCGGTGGTGATGTGAATGAATATGCAGCGGCGCCGAAAGGGGAGAAAGATAAGGGAGATGGCGAGAAATCTGGTGGTGGTGGGAACGAAGGAGGCGGTGGTGG
AAAGAAGCGTAACGGCAAGAAAGGCGGCGGTGGCGGTGGTGGTGGAAATGGAGGTGGAGAAGTGGGACGAGACGAAAATAGTGGCGGTGATGGCGAGGGTAAGAAAGGCGGAGATGGAGCTAAGCAGAGTAGTGGCGGGGACGGAGGAGCGGCAGAAGGCGGTGGCGGCGATGCTAGTGGAGGCAGTGGAGGAAAGAAGA
AGAAGGGCGGAAAAGGTGGAGATGGAATGACCGGCGAGTCTACTAGCTACGAAGAAACTGAATTAGCAGCAGCAACAGCAGCAGCAGGGGCAGGATTCGATCCTATGATGGGCGGGGTAGGGTACTGGGAAAACTCTATGCAGAGAACGGGTGTAGTACACAACCGGCAGATGTTGCAGTATAGACAACACTATCCTCCC
CCTATGTACGCTCCCACTGTGTATGCTAACCCTGCAAATCAAGCGGAGTATGCCACCATGTTCAGTGACGAAAATACTGGCGGCTGCTCGATTATGTAACTAAATCTCCATCAGTTCATTTGCAGGTTTCGTTTAGAGGAGTAGGACTGGAGGTTATCAGGGCATGCATGACGCAGTGACCATAGAATGGTTTTGAAACA
CCGTAGCCAGATTTAGCTGCTCGTAAATCTGCTAAAAGTAGCGGCGTACTTGCTTAGAGAGAAACGGAGAGGAGTTGTTGTGTGTTGTAATAAATGTGGTCCGTCAAACTCTAAATTTGCGTAGGTAGGGGAACAGACAAGGGCTAGTACGTTGGCCTCTTTTTATTTCCTGTATGTAATTATGTGAGTACTTGCAATAA
GACTAAGACTGCTTTGTATACCTATAATGTCAGATGGGAATCGAATATGAGATACACTGGCTCTCTGAATATTGCAATGGCATGGCAGGACTGGATTCACTGTATTGCACTCTGTATATTCAGTGTGTACCATATCAAATCATTCCTTTTATTTTTCCCAAACTTCACC

>TRINITY_DN111794_c0_g1_i5 from sample A1 / Example unspliced isoform:

AGTTGGGCGGCTGCTGACCGACCGCTGTATCTCACCAGAAAGATCAAATAACCGAACTGCAGAGGGTTTAATTGGCTGCCATTGAGTGGGAGTTTCTTGCTCCCTCACAAGGCACGAGGTAGAATTAATTCCTAGTCAAATTTGGACTCACGGAGTCCCGGAGCAGGCATGACAAAGGACGAAGATTTCAAGCTTATTAA
AACCCAGACTTGGGTTCTAAAGGTTTATATACACTGCGAGGGATGCAAGAAAAAGGTCAAGAAAGTTCTGCAGAGCATCGATGgtaagaagcgcgccgggatccggcaagcgtgcggataattgggtttgttttcttgttgtaagtgcaggtcgcgtgttcgaggctggttcaagatatttattaacggtgtggcttgtg
ttgcagGTGTGTTCACTACCTCCATTGA

Intron 5 GTAGGTTTTT ... CTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	1.05	94	0	1
A2	2.65	36.7	0	1
A3	4.05	71	0	3
A4	0	42	0	0
A5	0	25	0	0
A6	2.7	72	0	2
A7	1.73	227	1	4
A8	0.57	174	0	1
A9	1.13	175	0	2
A10	1.3	227	2	3

>TRINITY_DN38205_c0_g1_i5 from sample A8 / Example spliced isoform

GGGATGGGAGCTTTGATGGGAATTAAATGTGTAAGTTGGTTGACAGCTATGACACATGTGATTTCATGCTTTGGGTCCCTGAAAGAATTATTACTATTCCCTTTCAATATAGTGACAAGACAAGAGAAACAAGAAAAAAGATAACCAGCAGAGTGCTGCCTTTCTCTCTGCACATTTTTTTCTTACTGAGCGATCGTGTG
GCCTTTCCTCTGACTGAGCTGGCTGAGAGGGCTTGTACGAACAGTGGTTACCCTGTTGAGGTATTCTTCCTTTAATTATAAAGCACCCACTTACTTTTTCTGTAGGCCGTGTAAGCTGCTTTTTATGGTAACAGACTGGTCTCTGCTCATAGCTTTTCATTTTGCTCATCACGCGCCGTTGAAAAGGAGTTGGGCGGCTG
CTGACCGACCGCTGTATCTCACCAGAAAGATCAAATAACCGAACTGCAGAGGGTTTAATTGGCTGCCATTGAGTGGGAGTTTCTTGCTCCCTCACAAGGCACGAGGTAGAATTAATTCCTAGTCAAATTTGGACTCACGGAGTCCCGGAGCAGGCATGACAAAGGACGAAGATTTCAAGCTTATTAAAACCCAGACTTGG

GTTCTAAAGGTTTATATACACTGCGAGGGATGCAAGAAAAAGGTCAAGAAAGTTCTGCAGAGCATCGATGGTGTGTTCACTACCTCCATTGATGCTGCCGAGCAGAAGGTGACTGTTACAGGAAATGTGGCTTCCGCTGATATCTTGTTGAAGAAGTTGCTGAAATCCGGGAAGCATGCGGAGTTGTGGCCGGTGGCTCA
GAACAAACAGCAGAATAACGAGCCAAAGATTCCGAAGCAACAGAAAGAGCAGAAAGCGGGCAATGATGGCGGTGGTGATGTGAATGAATATGCAGCGGCGCCGAAAGGGGAGAAAGATAAGGGAGATGGCGAGAAATCTGGTGGTGGTGGGAACGAAGGAGGCGGTGGTGGAAAGAAGCGTAACGGCAAGAAAGGCGGCG
GTGGCGGTGGTGGTGGAAATGGAGGTGGAGAAGTGGGACGAGACGAAAATAGTGGCGGTGATGGCGAGGGTAAGAAAGGCGGAGATGGAGCTAAGCAGAGTAGTGGCGGGGACGGAGGAGCGGCAGAAGGCGGTGGCGGCGATGCTAGTGGAGGCAGTGGAGGAAAGAAGAAGAAGGGCGGAAAAGGTAAAGGTGGAGGT
GGAGGTGGAGGTGGAGGTGGAGATGGAATGACCGGCGAGTCTACTAGCTACGAAGAAACTGAATTAGCAGCAGCAACAGCAGCAGCAGGGGCAGGATTCGATCCTATGATGGGCGGGGTAGGGTACTGGGAAAACTCTATGCAGAGAACGGGTGTAGTACACAACCGGCAGATGTTGCAGTATAGACAACACTATCCTCC
CCCTATGTACGCTCCCACTGTGTATGCTAACCCTGCAAATCAAGCGGAGTATGCCACCATGTTCAGTGACGAAAATACTGGCGGCTGCTCGATTATGTAACTAAATCTCCATCAGTTCATTTGCAGGTTTCGTTTAGAGGAGTAGGACTGGAGGTTATCAGGGCATGCATGACGCAGTGACCATAGAATGGTTTTGAAAC
ACCGTAGCCAGATTTAGCTGCTCGTAAATCTGCTAAAAGTAGCGGCGTACTTGCTTAGAGAGAAACGGAGAGGAGTTGTTGTGTGTTGTAATAAATGTGGTCCGTCAAACTCTAAATTTGCGTAGGTAGGGGAACAGACAAGGGCTAGTACGTTGGCCTCTTTTTATTTCCTGTATGTAATTATGTGAGTACTTGCAATA
AGACTAAGACTGCTTTGTATACCTATAATGTCAGATGGGAATCGAATATGAGATACACTGGCTCTCTGAATATTGCAATGGCATGGCAGGACTGGATTCACTGTATTGCACTCTGTATATTCAGTGTGTACCATATCAAATCATTCCTTTTATTTTTCCCAAACTTCACCTACCCAAAAAAGTTAAAATAAGGTATATAT
ACATACTCTAGTATATATTACATGTATTTTGAAAACTTATCTGGTTATTATATATATTTTTGGACAGTTTAGCTTGGAAGTTTATCCATCATCATATAAATATACACACTCTGCACATTGTTTATTCAGCCCATCCATCTCCCCATCCCCATATATAAATATAATAATGAGTATTTTGAGGGTGT

>TRINITY_DN38205_c0_g1_i4 from sample A8 / Example unspliced isoform:

ATTTCAAGCTTATTAAAACCCAGgtaggtttttgaaaaaattccattcgttcctcgtgcttctagatctgggttttatagggatactttaacggtggttttcaatcgtcgtattgctgtttgcagACTTGGGTTCTAAAGGTTTATATACACTGCGAGGGATGCAAGAAAAAGGTCAAGAAAGTTCTGCAGAGCATCGAT
GGTAAGAAGCGCGCCGGGATCCGGCAAGCGTGCGGATAATTGGGTTTGTTTTCTTGTTGTAAGTGCAGGTCGCGTGTTCGAGGCTGGTTCAAGATATTTATTAACGGTGTGGCTTGTGTTGCAGGTGTGTTCACTACCTCCATTGATGCTGCCGAGCAGAAGGTGACTGTTACAGGAAATGTGGCTTCCGCTGATATCTT
GTTGAAGAAGTTGCTGAAATCCGGGAAGCATGCGGAGTTGTGGCCGGTGGCTCAGAACAAACAGCAGAATAACGAGCCAAAGATTCCGAAGCAACAGAAAGAGCAGAAAGCGGGCAATGATGGCGGTGGTGATGTGAATGAATATGCAGCGGCGCCGAAAGGGGAGAAAGATAAGGGAGATGGCGAGAAATCTGGTGGTG
GTGGGAACGAAGGAGGCGGTGGTGGAAAGAAGCGTAACGGCAAGAAAGGCGGCGGTGGCGGTGGTGGTGGAAATGGAGGTGGAGAAGTGGGACGAGACGAAAATAGTGGCGGTGATGGCGAGGGTAAGAAAGGCGGAGATGGAGCTAAGCAGAGTAGTGGCGGGGACGGAGGAGCGGCAGAAGGCGGTGGCGGCGATGCT
AGTGGAGGCAGTGGAGGAAAGAAGAAGAAGGGCGGAAAAGGTAAAGGTGGAGGTGGAGGTGGAGGTGGAGGTGGAGATGGAATGACCGGCGAGTCTACTAGCTACGAAGAAACTGAATTAGCAGCAGCAACAGCAGCAGCAGGGGCAGGATTCGATCCTATGATGGGCGGGGTAGGGTACTGGGAAAACTCTATGCAGAG
AACGGGTGTAGTACACAACCGGCAGATGTTGCAGTATAGACAACACTATCCTCCCCCTATGTACGCTCCCACTGTGTATGCTAACCCTGCAAATCAAGCGGAGTATGCCACCATGTTCAGTGACGAAAATACTGGCGGCTGCTCGATTATGTAACTAAATCTCCATCAGTTCATTTGCAGGTTTCGTTTAGAGGAGTAGG
ACTGGAGGTTATCAGGGCATGCATGACGCAGTGACCATAGAATGGTTTTGAAACACCGTAGCCAGATTTAGCTGCTCGTAAATCTGCTAAAAGTAGCGGCGTACTTGCTTAGAGAGAAACGGAGAGGAGTTGTTGTGTGTTGTAATAAATGTGGTCCGTCAAACTCTAAATTTGCGTAGGTAGGGGAACAGACAAGGGCT
AGTACGTTGGCCTCTTTTTATTTCCTGTATGTAATTATGTGAGTACTTGCAATAAGACTAAGACTGCTTTGTATACCTATAATGTCAGATGGGAATCGAATATGAGATACACTGGCTCTCTGAATATTGCAATGGCATGGCAGGACTGGATTCACTGTATTGCACTCTGTATATTCAGTGTGTACCATATCAAATCATTC
CTTTTATTTTTCCCAAACTTCACCTACCCAAAAAAGTTAAAATAAGGTATATATACATACTCTAGTATATATTACATGTATTTTGAAAACTTATCTGGTTATTATATATATTTTTGGACAGTTTAGCTTGGAAGTTTATCCATCATCATATAAATATACACACTCTGCACATTGTTTATTCAGCCCATCCATCTCCCCAT
CCCCATATATAAATATAATAATGAGTATTTTGAGGGTGT