NA ----> NA

Intron 1 GTAACCGGAA ... CGATTCCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.75	19	33	31
A2	75	14	42	42
A3	74.4	16	37	56
A4	75.28	11	32	35
A5	73.91	6	9	25
A6	87.78	11	68	90
A7	71.53	39	90	106
A8	79.31	12	32	60
A9	78.68	21	66	89
A10	72.47	34	77	102

>TRINITY_DN42981_c1_g1_i3 from sample A7 / Example spliced isoform

GGTAGATTCACAGGTTGACTTTTACTCAACATTCACCACCAACTTTAAGAACTTCAAACTGCTTTGGCAGAGAGCCAGGGACTCACAAACTGATCCTAGGAACTTCAAATATCACCGTTCTTTTACATTGCCGCTTGGAAGAGTGAAGACCCGAAATAAAACAAAGGCCATAAAACACAGGAAATGGCGACGGGGGCGCC
TTCGCTTTTTGCTGACTTACCTGAGGGTCTATTTCAATCCATGGACAAGTCACAGAGCATTTGTGGGCTCTCTGAAAATCTGCTCCCACACCCACCACCATGTGAGGAAGTGCTTCCTTCACAGGTTGTTCAATTGGAGAAATATGTCGCTGAGCCAGTGAACTTACCTGGAGGCCTTAGTTTGCTTAAGGGACGTGTAA
ACACTGCTGATGTATTTGGAGTTTCTAATTCAGATCTTGTTCCTGGGAAATATGAAGGGGGCCTGAAACTGTGGGAAAGCTCAACAGATCTAGTCAATGCCTTGAAACGCAAGATTCAAGATGGCCAGTTATCATTCAAGGGCAAGAAAGTATTGGAG

>TRINITY_DN42981_c1_g1_i1 from sample A7 / Example unspliced isoform:

GGTAGATTCACAGGTTGACTTTTACTCAACATTCACCACCAACTTTAAGAACTTCAAACTGCTTTGGCAGAGAGCCAGGGACTCACAAACTGATCCTAGGAACTTCAAATATCACCGTTCTTTTACATTGCCGCTTGGAAGAGTGAAGACCCGAAATAAAACAAAGGCCgtaaccggaattcaaaaccttcgttctatgt
ctggtttacagatttctggaaaaatattcaatttattcgagtgttaaaagcgtcccggtttcgattcccagATAAAACACAGGAAATGGCGACGGGGGCGCCTTCGCTTTTTGCTGACTTACCTGAGGGTCTATTTCAATCCATGGACAAGTCACAGAGCATTTGTGGGCTCTCTGAAAATCTGCTCCCACACCCACCAC
CATGTGAGGAAGTGCTTCCTTCACAGGTTGTTCAATTGGAGAAATATGTCGCTGAGCCAGTGAACTTACCTGGAGGCCTTAGTTTGCTTAAGGGACGTGTAAACACTGCTGATGTATTTGGAGTTTCTAATTCAGATCTTGTTCCTGGGAAATATGAAGGGGGCCTGAAACTGTGGGAAAGCTCAACAGATCTAGTCAAT
GCCTTGAAACGCAAGATTCAAGATGGCCAGTTATCATTCAAGGGCAAGAAAGTATTGGAGCTTGGTTGTGGGCATGGACTTCCTGCAATTTTTGCATGCCTGGAGGGTGCTTCAACTGTTCATTTTCAAGACTTTAATGCTGAAGTTTTAAGATGCCTTACTATACCAAATGTAAATTTAAACCTTGAGCAGGCTCACAA
GCAACAGGCTTGTCATACTGATAATACAATGATTCCAACAAAGACAACTTCTTTCACATCAGATATCCATTTCTATGCTGGAGATTGGGGAGAACTGCACACTTTGCTCTCTGTCGTAGCAACAGACCAGGTTGATTTGGCTGGAGAGGTTGACAAAAGTTTTCTTGGGGAGGATGTACCACTGGACAATTCTTGTTCTT
GGGATGGAAGTTCTGCTGATCAGGAAATACCAGCATGTTCAAATCAGATTCAGCAAGTGAGGTGTACTAGAAGGCATTCTCAATGTCATCCTTGTGAGCAGGCCAGGGATAGTGACCTTTCTGAAGGTGGTTATGACATTATTTTGATGGCAGAGACTGTCTATTCCCTCTCCTCTTTGCAAAAATTGTATGGGATTATT
AAAAAGTGTTTGCGACCACCTAATGGTGTTGTTTACTTGGCTGCTAAAAAACATTATTTTGGAGTAGGTGGTGGGACGCGACAATTCAAATCTTTGGTAGAAGAGGATGGTTAGTATAGATGTTAACATTAACAATTCTCACATTAGGTCTGTATTTACTATTTATTGATAGCAATGCCTTATATCAGGGATGTTTGAAT
GATTTGAAGGGATTTAAAAATCATGACCTTGT

Intron 2 GTTGTCCAGA ... AATTACCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.83	25	57	24
A2	71.83	10	34	17
A3	82.18	9	65	18
A4	82.46	5	25	22
A5	73.33	4	11	11
A6	75.65	66	241	169
A7	76.34	73	310	161
A8	69.67	91	242	176
A9	73.54	68	246	132
A10	79.65	46	233	127

>TRINITY_DN110102_c0_g3_i2 from sample A2 / Example spliced isoform

GAAAATTATACATCATGTTTCGACATGGCAGGTAGATTCACAGGTTGACTTTTACTCAACATTCACCACCAACTTTAAGAACTTCAAACTGCTTTGGCAGAGAGCCAGGGACTCACAAACTGATCCTAGGAACTTCAAATATCACCGTTCTTTTACATTGCCGCTTGGAAGAGTGAAGACCCGAAATAAAACAAAGGCCG
TAACCGGAATTCAAAACCTTCGTTCTATGTCTGGTTTACAGATTTCTGGAAAAATATTCAATTTATTCGAGTGTTAAAAGCGTCCCGGTTTCGATTCCCAGATAAAACACAGGAAATGGCGACGAGGGCGCCTTCGCTTTTTGCTGGCTTACCTGAGGGTCTATTTCAATCCATGGACAAGTCACAGAGCATTTGTGGGC
TCTCTGAAAATCTGCTCCCACACCCACCACCATGTGAGGAAGTGCTTCCTTCACAGGTTGTTCAATTGGAGAAATATGTCGCTGAGCCAGTGAACTTACCTGGAGGCCTTAGTTTGCTTAAGGGACGTGTAAACACTGCTGATGTATTTGGAGTTTCTAATTCAGATCTTGTTCCTGGGAAATATGAAGGGGGCCTGAAA
CTGTGGGAAAGCTCAACAGATCTAGTCAATGCCTTGAAACGCAAGATTCAAGATGGCCAGTTATCATTCAAGGGCAAGAAAGTATTGGAGCTTGGTTGTGGGCATGGACTTCCTGCAATTTTTGCATGCCTGGAGGGTGCTTCAACTGTTCATTTTCAAGACTTTAATGCTGAAGTTTTAAGATGCCTTACTATACCAAA
TGTAAATTTAAACCTTGAGCAGGCTCACAAGCAACAGGCTTGTCATACTGATAATACAATGATTCCAACAAAGACAACTTCTTTCACATCAGATATCCATTTCTATGCTGGAGATTGGGGAGAACTGCACACTTTGCTCTCTGTCGTAGCAACAGACCAGGTTGATTTGGCTGGAGAGGTTGACAAAAGTTTTCTTGGGG
AGGATGTACCACTGGATAATTCTTGTTCTTGGGATGGAAGTTCTTCTGATCAGGAAATACCAGCATGTTCAAATCAGATTCAGCAAGTGAGGTGTACTAGAAGGCATTCTCAATGTCATCCTTGTGAGCAGGCCAGGGATAGTGACCTTTCTGAAGGTGGTTATGACATTATTTTGATGGCAGAGACTGTCTATTCCCTC
TCCTCTTTGCAAAAATTGTATGGGATTATTAAAAAGTGTTTGCGACCACCTAATGGTGTTGTTTACTTGGCTGCTAAAAAACATTATTTTGGAGTAGGTGGTGGGACGCGACAATTCAAATCTTTGGTAGAAGAGGATGGTATTCTGGGAGCTCATTTGGTGGTGGACATTGCGGACGGTGCTTCTAATGTTCGAGAAAT
CTGGAAATTCTTTTTCAAGTAGAGTTTTAGCCAGGTAGGGTGGATATTAAGTAAAAGATGGGACATTGGGCAATTTCCGAGTCCCAGAAGTTGCCAAAGATTGCCCCAGCACAATTCAAGGAAAGATTCTTATGAAGGCTTGAAGTAACATAACTTATCTGGTTGGGTATTTTTTGCCAATGATCAGAAAGTGTTGCAAA
TACTATTTATTATCTAGAACAATCTTATTCAGTGGCATTATTTGTCAAGCAAATTGATTGATTATATTTTGGACTTCCATTTTACCGAGTATAATTCATGTTGTCCTGTGGTTCTGAGTTTACATTACCACTTTCTTGAGACAAATGAATGGGCAATGGTTGCCGGTTGAATGTAGATGATGTGAGTGTGATTCCTGA

>TRINITY_DN110102_c0_g3_i3 from sample A2 / Example unspliced isoform:

GAAAATTATACATCATGTTTCGACATGGCAGGTAGATTCACAGGTTGACTTTTACTCAACATTCACCACCAACTTTAAGAACTTCAAACTGCTTTGGCAGAGAGCCAGGGACTCACAAACTGATCCTAGGAACTTCAAATATCACCGTTCTTTTACATTGCCGCTTGGAAGAGTGAAGACCCGAAATAAAACAAAGGCCG
TAACCGGAATTCAAAACCTTCGTTCTATGTCTGGTTTACAGATTTCTGGAAAAATATTCAATTTATTCGAGTGTTAAAAGCGTCCCGGTTTCGATTCCCAGATAAAACACAGGAAATGGCGACGAGGGCGCCTTCGCTTTTTGCTGGCTTACCTGAGGGTCTATTTCAATCCATGGACAAGTCACAGAGCATTTGTGGGC
TCTCTGAAAATCTGCTCCCACACCCACCACCATGTGAGGAAGTGCTTCCTTCACAGGTTGTTCAATTGGAGAAATATGTCGCTGAGCCAGTGAACTTACCTGGAGGCCTTAGTTTGCTTAAGGGACGTGTAAACACTGCTGATGTATTTGGAGTTTCTAATTCAGATCTTGTTCCTGGGAAATATGAAGGGGGCCTGAAA
CTGTGGGAAAGCTCAACAGATCTAGTCAATGCCTTGAAACGCAAGATTCAAGATGGCCAGTTATCATTCAAGGGCAAGAAAGTATTGGAGCTTGGTTGTGGGCATGGACTTCCTGCAATTTTTGCATGCCTGGAGGGTGCTTCAACTGTTCATTTTCAAGACTTTAATGCTGAAGTTTTAAGATGCCTTACTATACCAAA
TGTAAATTTAAACCTTGAGCAGGCTCACAAGCAACAGGCTTGTCATACTGATAATACAATGATTCCAACAAAGACAACTTCTTTCACATCAGATATCCATTTCTATGCTGGAGATTGGGGAGAACTGCACACTTTGCTCTCTGTCGTAGCAACAGACCAGGTTGATTTGGCTGGAGAGGTTGACAAAAGTTTTCTTGGGG
AGGATGTACCACTGGATAATTCTTGTTCTTGGGATGGAAGTTCTTCTGATCAGGAAATACCAGCATGTTCAAATCAGATTCAGCAAGTGAGGTGTACTAGAAGGCATTCTCAATGTCATCCTTGTGAGCAGGCCAGGGATAGTGACCTTTCTGAAGGTGGTTATGACATTATTTTGATGGCAGAGACTGTCTATTCCCTC
TCCTCTTTGCAAAAATTGTATGGGATTATTAAAAAGTGTTTGCGACCACCTAATGGTGTTGTTTACTTGGCTGCTAAAAAACATTATTTTGGAGTAGGTGGTGGGACGCGACAATTCAAATCTTTGGTAGAAGAGGATGGTATTCTGGGAGCTCATTTGGTGGTGGACATTGCGGACGGTGCTTCTAATGTTCGAGAAAT
CTGGAAATTCTTTTTCAAGTAGAGTTTTAGCCAGgttgtccagatcagagttacctatcccagtattattgttaaacctattcctcgattatcattgcacatgaatgaagaaagcctaactcctcccttgtgcctttcaaattaccaagGTAGGGTGGATATTAAGTAAAAGATGGGACATTGGGCAATTTGTGAGTCCC
AGAAGTTGCCAAAGATTGCCCCAGCACAATTCAAGGAAAGATTCTTATGAAGGCTTGAAGTAACATAACTTATCTGGTTGGGTATTTTTTGCCAATGATCAGAAAGTGTTGCAAATACTATTTATTATCTAGAACAATCTTATTCAGTGGCATTATTTGTCAAGCAAATTGATTGATTATATTTTGGACTTCCATTTTAC
CGAGTATAATTCATGTTGTCCTGTGGTTCTGAGTTTACATTACCACTTTCTTGAGACAAATGAATGGGCAATGGTTGCCGGTTGAATGTAGATGATGTGAGTGTGATTCCTGA

Intron 3 GTTGTCCAGA ... ACCAAGGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.5	24	57	23
A2	71.43	10	34	16
A3	79.05	11	65	18
A4	81.82	5	25	20
A5	73.33	4	11	11
A6	75.05	67	241	162
A7	75.88	75	310	162
A8	70.46	87	242	173
A9	74.4	64	246	126
A10	79.42	46	233	122

>TRINITY_DN102542_c0_g1_i2 from sample A5 / Example spliced isoform

CAGGGACTCACAAACTGATCCTAGGAACTTCAAATATCACCGTTCTTTTACATTGCCGCTTGGAAGAGTGAAGACCCGAAATAAAACAAAGGCCGTAACCGGAATTCAAAACCTTCGTTTTATGTCTGGTTTACAGATTTCTGGAAAAATATTCAATTTATTCGAGTGTTAAAAGCGTCCCGGTTTCGATTTCCAGATAA
AACACAGGAAATGGCGACGGGGGCGCCTTCGCTTTTTGCTGGCTTACCTGAGGGTCTATTTCAATCCATGGACAAGTCACAGAGCATTTGTGGGCTCTCTGAAAATCTGCTCCCACACCCACCACCATGTGAGGAAGTGCTTCCTTCACAGGTTGTTCAATTGGAGAAATATGTCGCTGAGCCAGTGAACTTACCTGGAG
GCCTTAGTTTGCTTAAGGGACGTGTAAACACTGCTGATGTATTTGGAGTTTCTAATTCAGATCTTGTTCCTGGGAAATATGAAGGGGGCCTGAAACTGTGGGAAAGCTCAACAGATCTAGTCAATGCCTTGAAACGCAAGATTCAAGATGGCCAGTTATCATTCAAGGGCAAGAAAGTATTGGAGCTTGGTTGTGGGCAT
GGACTTCCTGCAATTTTTGCATGCCTGGAGGGTGCTTCAACTGTTCATTTTCAAGACTTTAATGCTGAAGTTTTAAGATGCCTTACTATACCAAATGTAAATTTAAACCTTGAGCAGGCTCACAAGCAACAGGCTTGTCATACTGATAATACAATGATTCCAACAAAGACAACTTCTTTCACATCAGATATCCATTTCTA
TGCTGGAGATTGGGGAGAACTGCACACTTTGCTCTCTGTCGTAGCAACAGACCAGGTTGATTTGGCTGGAGAGGTTGACAAAAGTTTTCTTGGGGAGGATGTACCACTGGACAATTCTTGTTCTTGGGATGGAAGTTCTTCTGATCAGGAAATACCAGCATGTTCAAATCAGATTCAGCAAGTGAGGTGTACTAGAAGGC
ATTCTCAATGTCATCCCTGTGAGCAGGCCAGGGATAGTGACCTTTCTGAAGGTGGTTATGACATTATTTTGATGGCAGAGACTGTCTATTCCCTCTCCTCTTTGCAAAAATTGTATGGGATTATTAAAAAGTGTTTGCGACCACCTAATGGTGTTGTTTACTTGGCTGCTAAAAAACATTATTTTGGAGTAGGTGGTGGG
ACGCGACAATTCAAATCTTTGGTAGAAGAGGATGGTATTCTGGGAGCTCATTTGGTGGTGGACATTGCGGACGGTGCTTCTAATGTTCGAGAAATCTGGAAATTCTTTTTCAAGTAGAGTTTTAGCCAGGGTGGATATTAAGTAAAAGATGGGACATTGGGCAATTTCCGAGTCCCAGAAGTTGCCAAAGATTGCCCCAG
CACAATTCAAGGAAAGATTCTTATGAAGGCTTGAAGTAACATAACTTATCTGGTTGGGTATTTTTTGCCAATGATCAGAAAGTGTTGCAAATACTATTTATTATCTAGAACAATCTTATTCAGTGGCATTATTTGTCAAGCAAATTGATTGATTATATTTTGGACTTCCATTTTACCGAGTATAATTCATGTTGTCCTGT
GGTTCTGAGTTTACATTACCACTTTCTTGAGACAAATGAATGGGCAATGGTTGCCAGTTGAATGTAGATGATTTGAGTGTGATTCCTGTACTGCTGTGGTGGATTCCCCTATGAGGCGACCCTCATATTAAAGAAATATATAACTGAGCTGTGTCCATGAAGGATCCC

>TRINITY_DN102542_c0_g1_i1 from sample A5 / Example unspliced isoform:

CAGGGACTCACAAACTGATCCTAGGAACTTCAAATATCACCGTTCTTTTACATTGCCGCTTGGAAGAGTGAAGACCCGAAATAAAACAAAGGCCGTAACCGGAATTCAAAACCTTCGTTTTATGTCTGGTTTACAGATTTCTGGAAAAATATTCAATTTATTCGAGTGTTAAAAGCGTCCCGGTTTCGATTTCCAGATAA
AACACAGGAAATGGCGACGGGGGCGCCTTCGCTTTTTGCTGGCTTACCTGAGGGTCTATTTCAATCCATGGACAAGTCACAGAGCATTTGTGGGCTCTCTGAAAATCTGCTCCCACACCCACCACCATGTGAGGAAGTGCTTCCTTCACAGGTTGTTCAATTGGAGAAATATGTCGCTGAGCCAGTGAACTTACCTGGAG
GCCTTAGTTTGCTTAAGGGACGTGTAAACACTGCTGATGTATTTGGAGTTTCTAATTCAGATCTTGTTCCTGGGAAATATGAAGGGGGCCTGAAACTGTGGGAAAGCTCAACAGATCTAGTCAATGCCTTGAAACGCAAGATTCAAGATGGCCAGTTATCATTCAAGGGCAAGAAAGTATTGGAGCTTGGTTGTGGGCAT
GGACTTCCTGCAATTTTTGCATGCCTGGAGGGTGCTTCAACTGTTCATTTTCAAGACTTTAATGCTGAAGTTTTAAGATGCCTTACTATACCAAATGTAAATTTAAACCTTGAGCAGGCTCACAAGCAACAGGCTTGTCATACTGATAATACAATGATTCCAACAAAGACAACTTCTTTCACATCAGATATCCATTTCTA
TGCTGGAGATTGGGGAGAACTGCACACTTTGCTCTCTGTCGTAGCAACAGACCAGGTTGATTTGGCTGGAGAGGTTGACAAAAGTTTTCTTGGGGAGGATGTACCACTGGACAATTCTTGTTCTTGGGATGGAAGTTCTTCTGATCAGGAAATACCAGCATGTTCAAATCAGATTCAGCAAGTGAGGTGTACTAGAAGGC
ATTCTCAATGTCATCCCTGTGAGCAGGCCAGGGATAGTGACCTTTCTGAAGGTGGTTATGACATTATTTTGATGGCAGAGACTGTCTATTCCCTCTCCTCTTTGCAAAAATTGTATGGGATTATTAAAAAGTGTTTGCGACCACCTAATGGTGTTGTTTACTTGGCTGCTAAAAAACATTATTTTGGAGTAGGTGGTGGG
ACGCGACAATTCAAATCTTTGGTAGAAGAGGATGGTATTCTGGGAGCTCATTTGGTGGTGGACATTGCGGACGGTGCTTCTAATGTTCGAGAAATCTGGAAATTCTTTTTCAAGTAGAGTTTTAGCCAGgttgtccagatcagagttacctatcccagtattattgttaaacctattcctcgattatcattgcacatgaa
tgaagaaagcctaactcctcccttgtgcctttcaaattaccaaggtagGGTGGATATTAAGTAAAAGATGGGACATTGGGCAATTTGTGAGTCCCAGAAGTTGCCAAAGATTGCCCCAGCACAATTCAAGGAAAGATTCTTATGAAGGCTTGAAGTAACATAACTTATCTGGTTGGGTATTTTTTGCCAATGATCAGAAA
GTGTTGCAAATACTATTTATTATCTAGAACAATCTTATTCAGTGGCATTATTTGTCAAGCAAATTGATTGATTATATTTTGGACTTCCATTTTACCGAGTATAATTCATGTTGTCCTGTGGTTCTGAGTTTACATTACCACTTTCTTGAGACAAATGAATGGGCAATGGTTGCCAGTTGAATGTAGATGATTTGAGTGTG
ATTCCTGTACTGCTGTGGTGGATTCCCCTATGAGGCGACCCTCATATTAAAGAAATATATAACTGAGCTGTGTCCATGAAGGATCCC