sp|Q9XER9|HUA2_ARATH ----> ENHANCER OF AG-4 protein 2

Intron 1 GTGTGTATTG ... GCAAAACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	13.11	49	2.8	8.1
A2	12.76	37	2.5	5.5
A3	19.75	53	4.8	14.3
A4	24.22	32	4.3	10.3
A5	13.16	15	2.5	2.5
A6	11.64	100	8	14.1
A7	11.14	143	9.5	19.4
A8	11.97	212	16.5	30.4
A9	9.83	150	9.3	17.5
A10	13.27	103	6.5	16.8

>TRINITY_DN41762_c0_g2_i6 from sample A6 / Example spliced isoform

ATTATGGGGTTCTTACGGATACAGGTCTCAAGTCGGATTTGCATGATAATCAAAAGAAAATAGACAACGGGGATGGTATGACAGTGCATGATAAGAGTGGTAAAGAGTTTGTTGTTATCTGTCATAAAACTGATGAATCAAAGCAACATGTGAAGTCTGTTTCAGTGCCTCCAAGGAAACAGGATGGTTTGAAGTCTGCT
TTAGACACTACTAATCAGAAAAAGGAATGGGTGGGGTGTGTTATGCATGCAGATTTGAAGTCTCATGTGCATGATAATCAGAAGAAACTGGGCAGTGAGGATGTTACGATAGTGCATACTAAGAATGCAGAAGAACAGGTTGTTACCTCCCAGAAAACTGATGAATCAAAGCAACATGGCAAGCCTGTATTAGTGCCTCC
CAGGATGTATGACAATTTGAAACCTGATCTAAGGCAAGGCAGTTGTAGTTTATCTCCAACTAAGAGTTATGGCGTTTTAGATCGCGCGAAAGTTGATGCTCATCAATTAGAGCATAAGGCTAAGTTGATCAAAACTAGAAAATATAATTTAACGCCAAATAAAGAAATGTGTCTTGATGTGTCCAAAAAAGATGACGGGC
AGTTGCATGCAGAAAAACCAAATCAACAGCATCAGCATAGGCATCCAGTTGTTTGCACGCCAGAGAAGGTTTCACATGCCAAACCTGGACGTGATAATTCTACACCAAAGTCAAAAGTCATCTCAAAATGCAATGCTGATATAAATGGTACACTTATGGTTAGTAGGTCTGAGAGTGGAGCATGTTCTTCGAAGTTACAT
TTAGATGAAGGCAACAAAATTAGGGAAGCGTATGAAGCTGCAAAAGAAGTAAAGCTCAAAATATCATCACAAAATTCTGGGCTGACTGCAGCATCTACATCTATGAAGTATCTAATTGCAGCTGCTCAGTCTAAACGACAGGCACGTTCTACTGTGGCACCTCCTCCTGTTGTGCCTGGAGAACTCGAAAAGTTTGCACC
TGTTCTAAGCCCTTCTCCAGCTCAAGGAAGAAGTTCTTGTCATCGTAGTTCACCCTCTCAGCCTTTACTGTATGGGAATTTGACAGTTGATGATACAAGAGATATGATGCTCCATGCTGAATCTAGGACCCCTGAAACAAGCACACAGCAGTGTGCTCCAACACAAATAACTGGTCCAGAAATGAGTGACGAGGGTAGAG
CTAGTTCTGGAAGAAAATCCGTTAGAGGGATACTTGATTCTGATGCAGAAGCAATTATGTCCAGGGATTCTTTCGAAGGAATGCTTGAAACATTGTCAAGGACGAGGGATAGTATCGGGAGAGCAACTCGCCTTGCTATTGAGTGTGCACAATATGGTATTGCAGGCGAGGTAGTGGAACTTCTTGTTCAGAAGTTGGAA
AATGAACCGAGTTATCATCGAAGAGTAGACCTGTTCTTTTTGGTTGATTCTATCACACAATGTTCGCGTGGGCAGAAAGGTATTGCTGGAGATACGTATCCATCTGCTGTCCAATCTGCACTTCCGCGGCTGTTGAGTGCAGCTGCTCCTCCTGGAAGTCAAGCACGTGAAAATCGCAGACAATGTTTAAAGGTTTTGAG
GCTTTGGCTTGAGCGCAAAATTCTTTCAGAGTCTGTCATAAGGCGGCACATAAATGAGATTGAGGCCTCCATTGAGGATAGATCAAACACTGTCTTACCTAGGCGTCCATCTCGATTACAGCGTGCTCTTGATGATCCAATCAGAGAAATGGAAGGCATGCTTGATGAGTATGGCAGCAATACATGTTTTCAATTGCCTG
GATTCCTAATGCCACAAATGTCTGAAGATGAAGAAGAGCCAAATGCAGGTGATGGGAAGAAAGTTGAAGAAAATGTTCTGATTGAAGCAACTGAAGCCCTGGAAGAAGGAGCCAACAGCTATAATCCAGAAAGATATCGACACATTCTTGAGGATGTTGAAGGGGAGCTTGAAATGGAAGATGTATCCCCATCTTCAGAA
TATGGTGCAAGTTCTTGTAAGAATGATACTCTTGGAGCAGATGCAAGACTAATTGGTCTCAGTGAAGCATTCTGTTCCCTAATGCCGAATGAAACAAATCAGTATCCTCCCTTGCCCTTGGATCCACCTCCACTACCAGAAGATATGCCCCCATCCCCTCCACCTCCACCTTCATCTCCACCACCTCCTTCACCACCTCC
ACCACCACCACCACCTCCACCTCCACCTCCACTTCCACTTTCTCCAGCCTATATCTCACGGCCTCTACTTTGTATACCCTATGCATCTCCAAATGATGTGCAACATCACATTTTACCTGGAACGCCACAACAACCTCATCATGGAACTAACCATTCAGGAGGGTCAGATTCAATTTTGTACAGTTCTCCTGCATACCCGA
TGTCTCAAAATCATAACTGCACAACTGCTAATGCCTTCAATTCTTCATCTTCTTATGGATTTTTACAGAGTAGTTTCCCTGGGGTTTCACATTGTGTACCCCTTGTCCAGCAAATGCATGGCAGTAATATGTCTTTTCCTCAGCAGTCTTACCGTCCACTTCCACCTACTCCTGCACCTTCCAATCAGTTCTCATTTGTA
CGGGCAGAAGTGAGGCATCAAGTGCAACAGTTATGGCCAGATCCATCTTTATCCCCTGTACTGGAAAGTAAGCAACCGGTACAAGATGAACATAGGGGCCATCTGCATGGTGACTTTGGTAACATGAGATTAGAGGAGCAACAACCTATCCAAGGAAGAAATGAAATCATGGAAACCTTGCACTCAGATCATATTGTGTG
GCCGAACCAAGGGATGCTCTTGTCTCCAACAGGAGCAGATAATGCTTGTGGTTTGTCTGGTTTTCATCCTCCACAACAGATCACAATGTCTCGTGGGTCTAGAAGTCCACTAGCAGCTAATCTCGTTCCAGCCCAGATAATACCATGCAATTCTGGCACTTCTACTCAAAATTGTTGGAGACCAAACTGATGTTCATGGT
TGGAGCAAGCGAGCTGTGCCTTTGGGATGTATATTTCTCAATTGTGTTAGAGAAGATTTATGCTGTGGAAAACTCTTAAGGTTGACTACAAGTAAGTTACAAGGATTTCAATATGGCCGCTGGACAGGAAGACATTCTTAATAACTGTACATATTGTACTCTGTGCCTCTATCAGCATTTGCAGATGCACTAAGGACATG
TTTTCTGAAAGAGAGATATTATGACTTTCCGAAGGGTTTTACATGATTGAACCATTCATTATTGTAGGCTTATTTTCTATGTCCTGATCTTGTATTTTTTTATTATCAAATGACTATAAACAGGGCATATTGTCCAGAGTCAGAGAATGGATGGTGTACGCAATTAAAATTTTCTCAAAATCTTTTTGTCAATGCCAAAT
TGAAGAATGTATTTAAACTGAGTTTAGTATTAACTTCTTCCTCCAAATCTGTTTAAAACCTAAAACATTTTGTTGCAAGTCGAATGTATCAAGTTACTAAAAATTAACCAGTTTATCTTCTGTCCTCAAGTGGCAATATAAGATAGAGCCACCAGGTTAGTGCCAGATTTGGATTGATATGGCTTTCACAATTAATATTT
GGCTTTCATTACTTGTTTAAATTGTGAGTTAAATTATACTTTGGATGTTATAAACGTATTCCCTAATTTTGTGATGCTTTTGTATTCACTTTGATTTAGTCTAAGTACACGTGATTTAAAT

>TRINITY_DN41762_c0_g2_i5 from sample A6 / Example unspliced isoform:

GATGTGCAACATCACATTTTACCTGGAACGCCACAACAACCTCATCATGGAACTAACCATTCAGGAGGGTCAGATTCAATTTTGTACAGTTCTCCTGCATACCCGATGTCTCAAAATCATAACTGCACAACTGCTAATGCCTTCAATTCTTCATCTTCTTATGGATTTTTACAGAGTAGTTTCCCTGGGGTTTCACATTG
TGTACCCCTTGTCCAGCAAATGCATGGCAGTAATATGTCTTTTCCTCAGCAGTCTTACCGTCCACTTCCACCTACTCCTGCACCTTCCAATCAGTTCTCATTTGTACGGGCAGAAGTGAGGCATCAAGTGCAACAGTTATGGCCAGATCCATCTTTATCCCCTGTACTGGAAAGTAAGCAACCGGTACAAGATGAACATA
GGGGCCATCTGCATGGTGACTTTGGTAACATGAGATTAGAGGAGCAACAACCTATCCAAGGAAGAAATGAAATCATGGAAACCTTGCACTCAGATCATATTGTGTGGCCGAACCAAGGGATGCTCTTGTCTCCAACAGGAGCAGATAATGCTTGTGGTTTGTCTGGTTTTCATCCTCCACAACAGATCACAATGTCTCGT
GGGTCTAGAAGTCCACTAGCAGgtgtgtattggcaaggttatgtggtaaactcaaagtatgatgcagacgtctttatcttccgaagtttcagtcaattttatgtcaattttttagcagaacacaaacactgacatgattctgtataaatgcaaaaccagCTAATCTCGTTCCAGCCCAGATAATACCATGCAATTCTGGC
ACTTCTACTCAAAATTGTTGGAGACCAAACTGATGTTCATGGTTGGAGCAAGCGAGCTGTGCCTTTGGGATGTATATTTCTCAATTGTGTTAGAGAAGATTTATGCTGTGGAAAACTCTTAAGGTTGACTACAAGTAAGTTACAAGGATTTCAATATGGCCGCTGGACAGGAAGACATTCTTAATAACTGTACATATTGT
ACTCTGTGCCTCTATCAGCATTTGCAGATGCACTAAGGACATGTTTTCTGAAAGAGAGATATTATGACTTTCCGAAGGGTTTTACATGATTGAACCATTCATTATTGTAGGCTTATTTTCTATGTCCTGATCTTGTATTTTTTTATTATCAAATGACTATAAACAGGGCATATTGTCCAGAGTCAGAGAATGGATGGTGT
ACGCAATTAAAATTTTCTCAAAATCTTTTTGTCAATGCCAAATTGAAGAATGTATTTAAACTGAGTTTAGTATTAACTTCTTCCTCCAAATCTGTTTAAAACCTAAAACATTTTGTTGCAAGTCGAATGTATCAAGTTACTAAAAATTAACCAGTTTATCTTCTGTCCTCAAGTGGCAATATAAGATAGAGCCACCAGGT
TAGTGCCAGATTTGGATTGATATGGCTTTCACAATTAATATTTGGCTTTCATTACTTGTTTAAATTGTGAGTTAAATTATACTTTGGATGTTATAAACGTATTCCCTAATTTTGTGATGCTTTTGTATTCACTTTGATTTAGTCTAAGTACACGTGATTTAAAT

Intron 2 GTGAGGGCAA ... GAATATTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	3.65	40.1	0	3
A2	5.6	25.4	3	0
A3	2.18	45	1	1
A4	11.1	16.1	3	1
A5	4.79	10.1	0	1
A6	3.03	113.4	1	6
A7	0.46	108.9	0	1
A8	2.51	176.4	7	2
A9	0	115.4	0	0
A10	3.29	74.4	5	0

>TRINITY_DN42786_c0_g1_i1 from sample A8 / Example spliced isoform

TGAAGAATCAAGTCTTAGTCCTCAGCAAGATGTCGAAAGAAAGCATCATGCCATGCCTGTACGAATGCTGCCCAGGAAACATGATGGTGGGGATGGTATAGTAGTGCATGTCTTAGAATGTACTAGTCAGGCAGAAGAGAAGACCCATGGAATTATGGATACAGATTTGAAGACTCATGTTCAAGACAATCAAATGATAT
TGATTAGTCAGGGTGACTTAAAAGGATATACTAAGGACACTGGACAATTAGATTTTACCTCTCAGGAACCTGAAGAATTAAAGCGACATGGCAAGTCCATGACAGTATCTCCAAAGAAGCATCATGGTTTGAGTTCAGCCCTGGAAAGTTTTAAATGGCCAGGAGAGAAGATGGGGGATATTATAAATACCAATTTGAAG
TCTGATATTCATAACAATCAAAAGAAAATGGATGGCCAGGGTCATGCAATCTTAGATGCTAAGACTGCTGAAGAATCAAGTCTTAGTCCTCCGCAGGATGTCAAAACAAAGCAACATGGCATGCCTGTACGAATGCTGCCCCAGGAACATGATGGTTCAAAGTTTGCTTCAGAGCACAATAATCAGGCAAAGGAGAACTT
GGGTGGTCTTATGGATGTAAATCTCAAGTCTGATGCACATGATAATCAAAAGAAAATGGGCAATGGGGATGGTATAATACATTCCTTGGAATGTACGAGTCAGGTAGAAGAGAAGACATGCAATATTAACTGCTCCGATTCAAAGTACCATGTAGAAGACAATCAAAAGGAATTCGTTGGTCAGGTTGATGTTATAGGAT
ACGCTAGGCATAATGAAGAATTGGATTTCACCTCTCGAAAACCTGAAGAATTAAAGCACTGTGACATGTCCATGACTGTGTCTCCCAAGAAGCATTATTGTTTGAAGCTGGCCTCAGGAGCATTCAATCAGGCAGAAGAGAAGATGGGGGATATTATGGATGCAAATTTGAAGTCTCATAATGATCAAAAGAAAAAGGAT
GGTCAGGGTCATGTACTATTGCCTGTTAAGACTGCTGAAGAATCAAGTCTTAGCTCTCAGAAAGCTGTCAAATCAAAGCACCATGCCATGCCTGTACCAATGCTGCCTGGGAAACATGGTGGTTCAAAGTATGGCTCAGAACAGTATAATCAGGCAAAGGAGAATTATGGGGTTCTTACGGATACAGGTCTCAAGTCGGA
TTTGCATGATAATCAAAAGAAAATAGACAACGGGGATGGTATGACAGTGCATGATAAGAGTGGTAAAGAGTTTGTTGTTATCTGTCATAAAACTGATGAATCAAAGCAACATGTGAAGTCTGTTTCAGTGCCTCCAAGGAAACAGGATGGTTTGAAGTCTACTTTAGACACTTCTAATCAGAAAAAGGAATGGGTGGGGT
GTGTTATGCATGCAGATTTGAAGTCTCATGTGCATGATAATCAGAAGAAACTGGGCAGTGAGGATGTTACGATAGTGCATACTAAGAATGCAGAAGAACAGGTTGTTACCTCCCAGAAAACTGATGAATCAAAGCAACATGGCAAGCCTGTATTAGTGCCTCCCAGGATGTATGACAATTTGAAACCTGATCTAAGGCAA
GGCAGTTGTAGTTTATCTCCAACTAAGAGTTATGGCGTTTTAGATCGCGCGAAAGTTGATGCTCATCAATTAGAGCATAAGGCTAAGTTGATCAAAACTAGAAAATATAATTTAACGCCAAATAAAGAAATGTGTCTTGATGTGTCCAAAAAAGATGACGGGCAGTTGCATGCAGAAAAACCAAATCAACAGCATCAGCA
TAGGCATCCAGTTGTTTGCACGCCAGAGAAGGTTTCACATGCCAAACCTGGACGTGATAATTCTACACCAAAGTCAAAAGTCATCTCAAAATGCAATGCTGATATAAATGGTACACTTATGGTTAGTAGGTCTGAGAGTGGAGCATGTTCTTCGAAGTTACATTTAGATGAAGGCAACAAAATTAGGGAAGCGTATGAAG
CTGCAAAAGAAGTAAAGCTCAAAATATCATCACAAAATTCTGGGCTGACTGCAGCATCTACATCTATGAAGTATCTAATTGCAGCTGCTCAGTCTAAACGACAGGCACGTTCTACTGTGGCACCTCCTCCTGTTGTGCCTGGAGAACTCGAAAAGTTTGCACCTGTTCTAAGCCCTTCTCCAGCTCAAGGAAGAAGTTCT
TGTCATCGTAGTTCACCCTCTCAGCCTTTACTGTATGGGAATTTGACAGTTGATGATACAAGAGATATGATGCTCCATGCTGAATCTAGGACCCCTGAAACAAGCACACAGCAGTGTGCTCCAACACAAATAACTGGTCCAGAAATGAGTGACGAGGGTAGAGCTAGTTCTGGAAGAAAATCCGTTAGAGGGATACTTGA
TTCTGATGCAGAAGCAATTATGTCCAGGGATTCTTTCGAAGGAATGCTTGAAACATTGTCAAGGACGAGGGATAGTATCGGGAGAGCAACTCGCCTTGCTATTGAGTGTGCACAATATGGTATTGCAGGCGAGGTAGTGGAACTTCTTGTTCAGAAGTTGGAAAATGAACCGAGTTATCATCGAAGAGTAGACCTGTTCT
TTTTGGTTGATTCTATCACACAATGTTCGCGTGGGCAGAAAGGTATTGCTGGAGATACGTATCCATCTGCTGTCCAATCTGCACTTCCGCGGCTGTTGAGTGCAGCTGCTCCTCCTGGAAGTCAAGCACGTGAAAATCGCAGACAATGTTTAAAGGTTTTGAGGCTTTGGCTTGAGCGCAAAATTCTTCCAGAGTCTGTC
ATAAGGCGGCACATAAATGAGATTGAGGCCTCCATTGAGGATAGATCAAACACTGTCTTACCTAGGCGTCCATCTCGATTACAGCGTGCTCTTGATGATCCAATCAGAGAAATGGAAGGCATGCTTGATGAGTATGGCAGCAATACATGTTTTCAATTGCCTGGATTCATAATGCCACAAATGTCTGAAGATGAAGAAGA
GCCAAATGCAGGTGATGGGAAGAAAGTTGAAGAAAATGTTCTGATTGAAGCAACTGAAGCCCTGGAAGAAGGAGCCAACAGCTATAATCCAGAAAGATATCGACACATTCTTGAGGATGTTGAAGGGGAGCTTGAAATGGAAGATGTATCCCCATCTTCAGAATATGGTGCAAGTTCTTGTAAGAATGATACTATTGGAG
CAGATGCAAGACTAATTGGTCTCAGTGAAGCATTCTGTTCCCTAATGCCGAATGAAACAAATCAGTATCCTCCCTTGCCCTTGGATCCACCTCCACTACCAGAAGATATGCCCCCATCCCCTCCACCTCCACCTTCATCTCCACCACCTCCTTCACCACCTCCACCACCACCACCACCTCCACCTCCACCTCCACTTCCA
CTTTCTCCAGCCTATATCTCACGGCCTCTACTTTGTATACCCTATGCATCTCCAAATGATGTGCAACATCACATTTTACCTGGAACGCCACAACAACCTCATCATGGAACTAACCATTCAGGAGGGTCAGATTCAATTTTGTACAGTTCTCCTGCATACCCGATGTCTCAAAATCATAACTGCACAACTGCTAATGCCTT
CAATTCTTCATCTTCTTATGGATTTTTACAGAGTAGTTTCCCTGGGGTTTCACATTGTGTACCCCTTGTCCAGCAAATGCATGGCAGTAATATGTCTTTTCCTCAGCAGTCTTACCGTCCACTTCCACCTACTCCTGCACCTTCCAATCAGTTCTCATTTGTACGGGCAGAAGTGAGGCATCAAGTGCAACAGTTATGGC
CAGATCCATCTTTATCCCCTGTACTGGAAAGTAAGCAACCGGTACAAGATGAACATAGGGGCCATCTGCATGGTGACTTTGGTAACATGAGATTAGAGGAGCAACAACCTATCCAAGGAAGAAATGAAATCATGGAAACCTTGCACTCAGGTTAACTTTATCATTTTCCTATCATAGAACTTTTAAGACTTGAAGTCATT
AATACTTGTTCATCTAGTTTTTGGAATGAAACCACGTTAAGTATTGGAGCATTCCAAAAACTCAATGGACAACACTACTAGCTGTGAGATTCTACACAATACAATCTTTATTACTTTCTCAGCTAGAGTTGTATTTCTACTCCTAGATTCACTATTCCGTGTTTCCCGTAGTAAAATACATGAAATGCTCTGCTTCTCTT
TTGGGTACAGACTCTGTAGTTTTCAAGAAAACTTAAGCAGCTCATTTTCATATTTCTCATTATGTATATTCAGATCATATTGTGTGGCCGAACCAAGGGATGCTCTTGTCTCCAACAGGAGCAGATAATGCTTGTGGTTTGTCTGGTTTTCATCCTCCACAACAGATCACAATGTCTCGTGGGTCTAGAAGTCCACTAGC
AGCTAATCTCGTTCCAGCCCAGATAATACCATGCAATTCTGGCACTTCTACTCAAAATTGTTGGAGACCAAACTGATGTTCATGGTTGGAGCAAGCGAGCTGTGCCTTTGGGATGTATATTTCTCAATTGTGTTAGAGAAGATTTATGCTGTGGAAAACTCTTAAGGTTGACTACAAGTAAGTTACAAGGATTTCAATAT
GGCCGCTGGACAGGAAGACATTCTTAATAACTGTACATATTGTACTCTGTGCCTCTATCAGCATTTGCAGATGCACTAAGGACATGTTTTCTGAAAGAGAGATATTATGACTTTCCGAAGGGTTTTACATGATTGAACCATTCATTATTGTAGGCTTATTTTCTATGTCCTGATCTTGTATTTTTTTATTATCAAATGAC
TATAAACAGGGCATATTGTCCAGAGTCAGAGAATGGATGGTGTACGCAATTAAAATTTTCTCAAAATCTTTTTGTCAATGCCAAATTGAAGAATGTATTTAAACTGAGTTTAGTATTAACTTCTTCCTCCAAATCTGTTTAAAACCTAAAACATTTTGTTGCAAGTCGAATGTATCAAGTTACTAAAAATTAACCAGTTT
ATCTTCTGTCCTCAAGTGGCAATATAAGATAGAGCCACCAGGTTAGTGCCAGATTTGGATTGATATGGCTTTCACAATTAATATTTGGC

>TRINITY_DN42786_c0_g1_i5 from sample A8 / Example unspliced isoform:

TGGGTCTAGAAGTCCACTAGCAGGTGTGTATTGGCAAGGTTATGTGGTAAACTCAAAGTATGATGCAGACGTCTTTATCTTCCGAAGTTTCAGTCAATTTTATGTCAATTTTTTAGCAGAACACAAACACTGACATGATTCTGTATAAATGCAAAACCAGCTAATCTCGTTCCAGCCCAGATAATACCATGCAATTCTGG
CACTTCTACTCAAAATTGTTGGAGACCAAACTGATGTTCATGgtgagggcaaaaagtgctacaagattaaagaacatagtatatggtttaccagattacttgatgcatgtgtcaatgtgttggtggatattccatggatttttgttatgcatttctatttattttgtagtccacattttttatttacatgttttctcttg
aatatttagGTTGGAGCAAGCGAGCTGTGCCTTTGGGATGTATATTTCTCAATTGTGTTAGAGAAGATTTATGCTGTGGAAAACTCTTAAGGTTGACTACAAG

Intron 3 GTTAACTTTA ... GTATATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.15	41	16	6
A2	27.27	32	13	11
A3	25.3	31	10	11
A4	24.8	47	14	17
A5	25	21	2	12
A6	27.85	57	18	26
A7	24.09	104	25	41
A8	24.02	174	36	74
A9	22.61	89	19	33
A10	23.08	80	24	24

>TRINITY_DN118959_c0_g5_i2 from sample A3 / Example spliced isoform

GTGGGTCTTATGGATGAAGATCTCAAGTCTGTTGTGCAGGATCATCAAAGGAAAATGAGCAATAAGGATGGTATAATAGTGCATGTCTTAGAATGTACTAGTCAGGCAGAAGAGAAGGCCTGTGAAATTATGGATACAGAGTTGAAGACTCATGTACAAGAGAATCAAATGATATTGATTAGTCAGGGTGACTTAAAAGG
ATATACTAAGGACACTGGACAATTAGATTTTACCTCTCAGGAACCTGACAAATTAAAGCGACATGACAAGTCCATGACAGTATCTCCAAAGAAGCATCATGGTTTGAGTTCAGCCCCGGAAAGTTTTAAATGGCCAGAAGAGAATATGGGGGATATTATAAATGCCAATTTGAAGTCTGATATTCATAACAATCAAAAGA
AAATGCATGGCCAGGGACATGCAATCTTAGATGCTACGACTGCTGAAGAATCAAGTCTTAGTCCTCAGCAAGATGTCGAAAGAAAGCATCATGCCATGCCTGTACGAATGCTGCCCAGGAAACATGATGGTGGGGATGGTATAGTAGTGCATGTCTTAGAATGTACTAGTCAGGCAGAAGAGAAGACCCATGGAATTATG
GATACAGATTTGAAGACTCATGTTCAAGACAATCAAATGATATTGATTAGTCAGGATGACTTAGAAGGATATACTAAGGACACTGGAAATTTAGATTTTACCTCTCAGGAACCTGAAGAATTAAAGCGACATGGCAAGTCCATGACAGTATCTCCAAAGAAGCATCATGGTTTGAGTTCAGCCCTGGAAAGTTTTAAATG
GCCAGGAGAGAAGATGGGGGATATTATAAATACCAATTTGAAGTCTGATATTCATAACAATCAAAAGAAAATGGATGGCCAGGGTCATGCAATCTTAGATGCTAAGACTGCTGAAGAATCAAGTCTTAGTCCTCCGCAGGATGTCAAAACAAAGCAACATGGCATGCCTGTACGAATGCTGCCCAGGAAACATGATGGTT
CAAAGTTTGCTTCAGAGCACAATAATCAGGCAAAGGAGAACTTGGGTGGTCTTATGGATGTAAATCTCAAGTCTGATGCACATGATAATCAAAAGAAAATGGGCAATGGGGATGGTATAATACATTCCTTGGAATGTACGAGTCAGGTAGAAGAGAAGACATGCAATATTAACTGCTCCGATTCAAAGTACCATGTAGAA
GACAATCAAAAGGAATTCGTTGGTCAGGTTGATGTTATAGGATACGCTAGGCATAATGAAGAATTGGATTTCACCTCTCGAAAACCTGAAGAATTAAAGCACTGTGACATGTCCATGACTGTGTCTCCCAAGAAGCATTATTGTTTGAAGCTGGCCTCAGGAGCATTCAATCAGGCAGAAGAGAAGATGGGGGATATTAT
GGATGCAAATTTGAAGTCTCATAATGATCAAAAGAAAAAGGATGGTCAGGGTCATGTACTATTGCCTGTTAAGACTGCTGAAGAATCAAGTCTTAGCTCTCAGAAAGCTGTCAAATCAAAGCAGCATGCCATGCCTGTACCAATGCTGCCTGGGAAACATGGTGGTTCAAAGTATGGCTCAGAACAGTATAATCAGGCAA
AGGAGAATTATGGGGTTCTTACGGATACAGGTCTCAAGTCGGATTTGCATGATAATCAAAAGAAAATAGACAACGGGGATGGTATGACAGTGCATGATAAGAGTGGTAAAGAGTTTGTTGTTATCTGTCATAAAACTGATGAATCAAAGCAACATGTGAAGTCTGTTTCAGTGCCTCCAAGGAAACAGGATGGTTTGAAG
TCTACTTTAGACACTTCTAATCAGAAAAAGGAATGGGTGGGGTGTGTTATGCATGCAGATTTGAAGTCTCATGTGCATGATAATCAGAAGAAACTGGGCAGTGAGGATGTTACGATAGTGCATACTAAGAATGCAGAAGAACAGGTTGTTACCTCCCAGAAAACTGATGAATCAAAGCAACATGGCAAGCCTGTATTAGT
GCCTCCCAGGATGTATGACAATTTGAAACCTGATCTAAGGCAAGGCAGTTGTAGTTTATCTCCAACTAAGAGTTATGGCGTTTTAGATCGCGCGAAAGTTGATGCTCATCAATTAGAGCATAAGGCTAAGTTGATCAAAACTAGAAAATATAATTTAACGCCAAATAAAGAAATGTGTCTTGATGTGTCCAAAAAAGATG
ACGGGCAGTTGCATGCAGAAAAACCAAATCAACAGCATCAGCATAGGCATCCAGTTGTTTGCACGCCAGAGAAGGTTTCACATGCCAAACCTGGACGTGATAATTCTACACCAAAGTCAAAAGTCATCTCAAAATGCAATACTGATATAAATGGTACACTTATGGTTAGTAGGTCTGAGAGTGGAGCATGTTCTTCGAAG
TTACATTTAGATGAAGGCAACAAAATTAGGGAAGCGTATGAAGCTGCAAAAGAAGTAAAGCTCAAAATATCATCACAAAATTCTGGGCTGACTGCAGCATCTACATCTATGAAGTATCTAATTGCAGCTGCTCAGTCTAAACGACAGGCACGTTCTACTGTGGCACCTCCTCCTGTTGTGCCTGGAGAACTCGAAAAGTT
TGCACCTGTTCTAAGCCCTTCTCCAGCTCAAGGAAGAAGTTCTTGTCATCGTAGTTCACCCTCTCAGCCTTTACTGTATGGGAATTTGACAGTTGATGATACAAGAGATATGATGCTCCATGCTGAATCTAGGACCCCTGAAACAAGCACACAGCAGTGTGCTCCAACACAAATAACTGGTCCAGAAATGAGTGACGAGG
GTAGAGCTAGTTCTGGAAGAAAATCCGTTAGAGGGATACTTGATTCTGATGCAGAAGCAATTATGTCCAGGGATTCTTTCGAAGGAATGCTTGAAACATTGTCAAGGACGAGGGATAGTATCGGGAGAGCAACTCGCCTTGCTATTGAGTGTGCACAATATGGTATTGCAGGCGAGGTAGTGGAACTTCTTGTTCAGAAG
TTGGAAAATGAACCGAGTTATCATCGAAGAGTAGACCTGTTCTTTTTGGTTGATTCTATCACACAATGTTCGCGTGGGCAGAAAGGTATTGCTGGAGATACGTATCCATCTGCTGTCCAATCTGCACTTCCGCGGCTGTTGAGTGCAGCTGCTCCTCCTGGAAGTCAAGCACGTGAAAATCGCAGACAATGTTTAAAGGT
TTTGAGGCTTTGGCTTGAGCGCAAAATTCTTCCAGAGTCTGTCATAAGGCGGCACATAAATGAGATTGAGGCCTCCATTGAGGATAGATCAAACACTGTCTTACCTAGGCGTCCATCTCGATTACAGCGTGCTCTTGATGATCCAATCAGAGAAATGGAAGGCATGCTTGATGAGTATGGCAGCAATACATGTTTTCAAT
TGCCTGGATTCATAATGCCACAAATGTCTGAAGATGAAGAAGAGCCAAATGCAGGTGATGGGAAGAAAGTTGAAGAAAATGTTCTGATTGAAGCAACTGAAGCCCTGGAAGAAGGAGCCAACAGCTATAATCCAGAAAGATATCGACACATTCTTGAGGATGTTGAAGGGGAGCTTGAAATGGAAGATGTATCCCCATCT
TCAGAATATGGTGCAAGTTCTTGTAAGAATGATACTCTTGGAGCAGATGCAAGACTAATTGGTCTCAGTGAAGCATTCTGTTCCCTAATGCCGAATGAAACAAATCAGTATCCTCCCTTGCCCTTGGATCCACCTCCACTACCAGAAGATATGCCCCCATCCCCTCCACCTCCACCTTCATCTCCACCACCTCCTTCACC
ACCTCCACCACCACCACCACCTCCACCTCCACCTCCACTTCCACTTTCTCCAGCCTATATCTCACGGCCTCTACTTTGTATACCCTATGCATCTCCAAATGATGTGCAACATCACATTTTACCTGGAACGCCACAACAACCTCATCATGGAACTAACCATTCAGGAGGGTCAGATTCAATTTTGTACAGTTCTCCTGCAT
ACCCGATGTCTCAAAATCATAACTGCACAACTGCTAATGCCTTCAATTCTTCATCTTCTTATGGATTTTTACAGAGTAGTTTCCCTGGGGTTTCACATTGTGTACCCCTTGTCCAGCAAATGCATGGCAGTAATATGTCTTTTCCTCAGCAGTCTTACCGTCCACTTCCACCTACTCCTGCACCTTCCAATCAGTTCTCA
TTTGTACGGGCAGAAGTGAGGCATCAAGTGCAACAGTTATGGCCAGATCCATCTTTATCCCCTGTACTGGAAAGTAAGCAACCGGTACAAGATGAACATAGGGGCCATCTGCATGGTGACTTTGGTAACATGAGATTAGAGGAGCAACAACCTATCCAAGGAAGAAATGAAATCATGGAAACCTTGCACTCAGATCATAT
TGTGTGGCCGAACCAAGGGATGCTCTTGTCTCCAACAGGAGCAGATAATGCTTGTGGTTTGTCTGGTTTTCATCCTCCACAACAGATCACAATGTCTCGTGGGTCTAGAAGTCCACTAGCAGCTAATCTCGTTCCAGCCCAGATAATACCATGCAATTCTGGCACTTCTACTCAAAATTGTTGGAGACCAAACTGATGTT
CATGGTTGGAGCAAGCGAGCTGTGCCTTTGGGATGTATATTTCTCAATTGTGTTAGAGAAGATTTATGCTGTGGAAAACTCTTAAGGTTGACTACAAGTAAGTTACAAGGATTTCAATATGGCCGCTGGACAGGAAGACATTCTTAATAACTGTACATATTGTACTCTGTGCCTCTATCAGCATTTGCAGATGCACTAAG
GACATGTTTTCTGAAAGAGAGATATTATGACTTTCCGAAGGGTTTTACATGATTGAACCATTCATTATTGTAGGCTTATTTTCTATGTCCTGATCTTGTATTTTTTTATTATCAAATGACTATAAACAGGGCATATTGTCCAGAGTCAGAGAATGGATGGTGTACGCAATTAAAATTTTCTCAAAATCTTTTTGTCAATG
CCAAATTGAAGAATGTATTTAAACTGAGTTTAGTATTAACTTCTTCCTCCAAATCTGTTTAAAACCTAAAACATTTTGTTGCAAGTCGAATGTATCAAGTTACTAAAAATTAACCAGTTTATCTTCTGTCCTCAAGTGGCAATATAAGATAGAGCCACCAGGTTAGTGCCAGATTTGGATTGATATGGCTTTCACAATTA
ATATTTGGCTTTCATTACTTGTTTAAATTGTGAGTTAAATTATACTTTGGATGTTATAAACGTATTCCCTAATTTTGTGATGCTTTTGTATTCACTTTGATTTAGTCTAAGTACACGTGATTTAAATAGAATTCACAATGCATGGATGTTATTGGTGGACAAAGGGACTTGGGGATCAATATAGTATCTCTAAGAAGCCA
AGTAACCCTTAAGTTTTACTAAATATGATTACTTGGTATCATGTCAGGGCATTTTGTTTGAAGTGCACACAGCATTAAGGGGATGCCTGAGGCATTTCTGGGAAACACCTGCTTGTTGAGAGGTATCCCTCTGTGCATGAAACATCTGCAGTGGGGACGTCACCCCCAGCAAAAAATAAAATAAAATACTCAATTAAATG
TGATTTGATACCACGGCCTTGATTTCAAGCATTAGGAATGTTCCTGTTCCAGATGCAAATCATTAGTTAAGCATGCCTGAGTATGGATTCTGGAATTATGACAAATCATTGCCTCCACGCATTTTTATTATATATTATGTAGGAATTTCAGATTAAGACTGCCTATTCTACTAATTGGAAGAATATGTGCCCTTTGGGGA
ATGCCAGAGTTTATGTGGAATTTTTCTTACAGTCTTGGAGATTATGCTTTTACTATACTATTTAACTACATACAAAGAGAAATATAGCAATGTTATACCATTGCCTTTCTTGCTGTGCCAGAATTGAAGTGTGACAAATCTATGCCTCTAAGCATTTTTATTATATATTATTTATGAATTTCAGATTAAAAGTTTGTTAT
TCTACGGAATGGAAAAACGTGTGGCGTTTCTGGAATATCATAGAATTTATGCAGAATTTTAATACAGACTTGGAGATAATGTTTAGACTATGCTATTTAACTACATGTGCAGAGAAATACA

>TRINITY_DN118959_c0_g5_i9 from sample A3 / Example unspliced isoform:

GTGGGTCTTATGGATGAAGATCTCAAGTCTGTTGTGCAGGATCATCAAAGGAAAATGAGCAATAAGGATGGTATAATAGTGCATGTCTTAGAATGTACTAGTCAGGCAGAAGAGAAGGCCTGTGAAATTATGGATACAGAGTTGAAGACTCATGTACAAGAGAATCAAATGATATTGATTAGTCAGGGTGACTTAAAAGG
ATATACTAAGGACACTGGACAATTAGATTTTACCTCTCAGGAACCTGACAAATTAAAGCGACATGACAAGTCCATGACAGTATCTCCAAAGAAGCATCATGGTTTGAGTTCAGCCCCGGAAAGTTTTAAATGGCCAGAAGAGAATATGGGGGATATTATAAATGCCAATTTGAAGTCTGATATTCATAACAATCAAAAGA
AAATGCATGGCCAGGGACATGCAATCTTAGATGCTACGACTGCTGAAGAATCAAGTCTTAGTCCTCAGCAAGATGTCGAAAGAAAGCATCATGCCATGCCTGTACGAATGCTGCCCAGGAAACATGATGGTGGGGATGGTATAGTAGTGCATGTCTTAGAATGTACTAGTCAGGCAGAAGAGAAGACCCATGGAATTATG
GATACAGATTTGAAGACTCATGTTCAAGACAATCAAATGATATTGATTAGTCAGGATGACTTAGAAGGATATACTAAGGACACTGGAAATTTAGATTTTACCTCTCAGGAACCTGAAGAATTAAAGCGACATGGCAAGTCCATGACAGTATCTCCAAAGAAGCATCATGGTTTGAGTTCAGCCCTGGAAAGTTTTAAATG
GCCAGGAGAGAAGATGGGGGATATTATAAATACCAATTTGAAGTGATATTCATAACAATCAAAAGAAAATGGATGGCCAGGGTCATGCAATCTTAGATGCTAAGACTGCTGAAGAATCAAGTCTTAGTCCTCCGCAGGATGTCAAAACAAAGCAACATGGCATGCCTGTACGAATGCTGCCCAGGAAACATGATGGTTCA
AAGTTTGCTTCAGAGCACAATAATCAGGCAAAGGAGAACTTGGGTGGTCTTATGGATGTAAATCTCAAGTCTGATGCACATGATAATCAAAAGAAAATGGGCAATGGGGATGGTATAATACATTCCTTGGAATGTACGAGTCAGGTAGAAGAGAAGACATGCAATATTAACTGCTCCGATTCAAAGTACCATGTAGAAGA
CAATCAAAAGGAATTCGTTGGTCAGGTTGATGTTATAGGATACGCTAGGCATAATGAAGAATTGGATTTCACCTCTCGAAAACCTGAAGAATTAAAGCACTGTGACATGTCCATGACTGTGTCTCCCAAGAAGCATTATTGTTTGAAGCTGGCCTCAGGAGCATTCAATCAGGCAGAAGAGAAGATGGGGGATATTATGG
ATGCAAATTTGAAGTCTCATAATGATCAAAAGAAAAAGGATGGTCAGGGTCATGTACTATTGCCTGTTAAGACTGCTGAAGAATCAAGTCTTAGCTCTCAGAAAGCTGTCAAATCAAAGCAGCATGCCATGCCTGTACCAATGCTGCCTGGGAAACATGGTGGTTCAAAGTATGGCTCAGAACAGTATAATCAGGCAAAG
GAGAATTATGGGGTTCTTACGGATACAGGTCTCAAGTCGGATTTGCATGATAATCAAAAGAAAATAGACAACGGGGATGGTATGACAGTGCATGATAAGAGTGGTAAAGAGTTTGTTGTTATCTGTCATAAAACTGATGAATCAAAGCAACATGTGAAGTCTGTTTCAGTGCCTCCAAGGAAACAGGATGGTTTGAAGTC
TACTTTAGACACTTCTAATCAGAAAAAGGAATGGGTGGGGTGTGTTATGCATGCAGATTTGAAGTCTCATGTGCATGATAATCAGAAGAAACTGGGCAGTGAGGATGTTACGATAGTGCATACTAAGAATGCAGAAGAACAGGTTGTTACCTCCCAGAAAACTGATGAATCAAAGCAACATGGCAAGCCTGTATTAGTGC
CTCCCAGGATGTATGACAATTTGAAACCTGATCTAAGGCAAGGCAGTTGTAGTTTATCTCCAACTAAGAGTTATGGCGTTTTAGATCGCGCGAAAGTTGATGCTCATCAATTAGAGCATAAGGCTAAGTTGATCAAAACTAGAAAATATAATTTAACGCCAAATAAAGAAATGTGTCTTGATGTGTCCAAAAAAGATGAC
GGGCAGTTGCATGCAGAAAAACCAAATCAACAGCATCAGCATAGGCATCCAGTTGTTTGCACGCCAGAGAAGGTTTCACATGCCAAACCTGGACGTGATAATTCTACACCAAAGTCAAAAGTCATCTCAAAATGCAATACTGATATAAATGGTACACTTATGGTTAGTAGGTCTGAGAGTGGAGCATGTTCTTCGAAGTT
ACATTTAGATGAAGGCAACAAAATTAGGGAAGCGTATGAAGCTGCAAAAGAAGTAAAGCTCAAAATATCATCACAAAATTCTGGGCTGACTGCAGCATCTACATCTATGAAGTATCTAATTGCAGCTGCTCAGTCTAAACGACAGGCACGTTCTACTGTGGCACCTCCTCCTGTTGTGCCTGGAGAACTCGAAAAGTTTG
CACCTGTTCTAAGCCCTTCTCCAGCTCAAGGAAGAAGTTCTTGTCATCGTAGTTCACCCTCTCAGCCTTTACTGTATGGGAATTTGACAGTTGATGATACAAGAGATATGATGCTCCATGCTGAATCTAGGACCCCTGAAACAAGCACACAGCAGTGTGCTCCAACACAAATAACTGGTCCAGAAATGAGTGACGAGGGT
AGAGCTAGTTCTGGAAGAAAATCCGTTAGAGGGATACTTGATTCTGATGCAGAAGCAATTATGTCCAGGGATTCTTTCGAAGGAATGCTTGAAACATTGTCAAGGACGAGGGATAGTATCGGGAGAGCAACTCGCCTTGCTATTGAGTGTGCACAATATGGTATTGCAGGCGAGGTAGTGGAACTTCTTGTTCAGAAGTT
GGAAAATGAACCGAGTTATCATCGAAGAGTAGACCTGTTCTTTTTGGTTGATTCTATCACACAATGTTCGCGTGGGCAGAAAGGTATTGCTGGAGATACGTATCCATCTGCTGTCCAATCTGCACTTCCGCGGCTGTTGAGTGCAGCTGCTCCTCCTGGAAGTCAAGCACGTGAAAATCGCAGACAATGTTTAAAGGTTT
TGAGGCTTTGGCTTGAGCGCAAAATTCTTCCAGAGTCTGTCATAAGGCGGCACATAAATGAGATTGAGGCCTCCATTGAGGATAGATCAAACACTGTCTTACCTAGGCGTCCATCTCGATTACAGCGTGCTCTTGATGATCCAATCAGAGAAATGGAAGGCATGCTTGATGAGTATGGCAGCAATACATGTTTTCAATTG
CCTGGATTCATAATGCCACAAATGTCTGAAGATGAAGAAGAGCCAAATGCAGGTGATGGGAAGAAAGTTGAAGAAAATGTTCTGATTGAAGCAACTGAAGCCCTGGAAGAAGGAGCCAACAGCTATAATCCAGAAAGATATCGACACATTCTTGAGGATGTTGAAGGGGAGCTTGAAATGGAAGATGTATCCCCATCTTC
AGAATATGGTGCAAGTTCTTGTAAGAATGATACTCTTGGAGCAGATGCAAGACTAATTGGTCTCAGTGAAGCATTCTGTTCCCTAATGCCGAATGAAACAAATCAGTATCCTCCCTTGCCCTTGGATCCACCTCCACTACCAGAAGATATGCCCCCATCCCCTCCACCTCCACCTTCATCTCCACCACCTCCTTCACCAC
CTCCACCACCACCACCACCTCCACCTCCACCTCCACTTCCACTTTCTCCAGCCTATATCTCACGGCCTCTACTTTGTATACCCTATGCATCTCCAAATGATGTGCAACATCACATTTTACCTGGAACGCCACAACAACCTCATCATGGAACTAACCATTCAGGAGGGTCAGATTCAATTTTGTACAGTTCTCCTGCATAC
CCGATGTCTCAAAATCATAACTGCACAACTGCTAATGCCTTCAATTCTTCATCTTCTTATGGATTTTTACAGAGTAGTTTCCCTGGGGTTTCACATTGTGTACCCCTTGTCCAGCAAATGCATGGCAGTAATATGTCTTTTCCTCAGCAGTCTTACCGTCCACTTCCACCTACTCCTGCACCTTCCAATCAGTTCTCATT
TGTACGGGCAGAAGTGAGGCATCAAGTGCAACAGTTATGGCCAGATCCATCTTTATCCCCTGTACTGGAAAGTAAGCAACCGGTACAAGATGAACATAGGGGCCATCTGCATGGTGACTTTGGTAACATGAGATTAGAGGAGCAACAACCTATCCAAGGAAGAAATGAAATCATGGAAACCTTGCACTCAGgttaacttt
atcattttcctatcatagaacttttaagacttgaagtcattaatacttgttcatctagtttttggaatgaaaccacgttaagtattggagcattccaaaaactcaatggacaacactactagctgtgagattctacacaatacaatctttattactttctcagctagagttgtatttctactcctagattcactattccg
tgtttcccgtagtaaaatacatgaaatgctctgcttctcttttgggtacagactctgtagttttcaagaaaactaaagcagctcattttcatatttctcattatgtatattcagATCATATTGTGTGGCCGAACCAAGGGATGCTCTTGTCTCCAACAGGAGCAGATAATGCTTGTGGTTTGTCTGGTTTTCATCCTCCA
CAACAGATCACAATGTCTCGTGGGTCTAGAAGTCCACTAGCAGCTAATCTCGTTCCAGCCCAGATAATACCATGCAATTCTGGCACTTCTACTCAAAATTGTTGGAGACCAAACTGATGTTCATGGTTGGAGCAAGCGAGCTGTGCCTTTGGGATGTATATTTCTCAATTGTGTTAGAGAAGATTTATGCTGTGGAAAAC
TCTTAAGGTTGACTACAAGTAAGTTACAAGGATTTCAATATGGCCGCTGGACAGGAAGACATTCTTAATAACTGTACATATTGTACTCTGTGCCTCTATCAGCATTTGCAGATGCACTAAGGACATGTTTTCTGAAAGAGAGATATTATGACTTTCCGAAGGGTTTTACATGATTGAACCATTCATTATTGTAGGCTTAT
TTTCTATGTCCTGATCTTGTATTTTTTTATTATCAAATGACTATAAACAGGGCATATTGTCCAGAGTCAGAGAATGGATGGTGTACGCAATTAAAATTTTCTCAAAATCTTTTTGTCAATGCCAAATTGAAGAATGTATTTAAACTGAGTTTAGTATTAACTTCTTCCTCCAAATCTGTTTAAAACCTAAAACATTTTGT
TGCAAGTCGAATGTATCAAGTTACTAAAAATTAACCAGTTTATCTTCTGTCCTCAAGTGGCAATATAAGATAGAGCCACCAGGTTAGTGCCAGATTTGGATTGATATGGCTTTCACAATTAATATTTGGCTTTCATTACTTGTTTAAATTGTGAGTTAAATTATACTTTGGATGTTATAAACGTATTCCCTAATTTTGTG
ATGCTTTTGTATTCACTTTGATTTAGTCTAAGTACACGTGATTTAAATAGAATTCACAATGCATGGATGTTATTGGTGGACAAAGGGACTTGGGGATCAATATAGTATCTCTAAGAAGCCAAGTAACCCTTAAGTTTTACTAAATATGATTACTTGGTATCATGTCAGGGCATTTTGTTTGAAGTGCACACAGCATTAAG
GGGATGCCT

Intron 4 GTAAGTATTG ... GTTTTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.12	44	14	11
A2	22.86	27	11	5
A3	21.57	60	21	12
A4	26.15	24	4	13
A5	25	9	4	2
A6	21.43	11	4	2
A7	12.5	14	1	3
A8	21.21	13	1	6
A9	7.69	12	1	1
A10	25.33	28	5	14

>TRINITY_DN114572_c1_g1_i2 from sample A2 / Example spliced isoform

AGTAGATCACACAGCAGTAGCACCAAAAGAAGAAGGCGGCATGGGCAAATTATCGAGTTCAAGTGGAACCGCCATGGCGGAGCCCCGTAAATTGAACCTTCGAGCTTCCAGGAAGCTAAAATCCTGCTGAATTACAGCCTCACTTGCCACTAAAAAGAACAAACGATACACACATAGTGTGAAAGACGTGTGGAGTTTGA
ACTGCTCGGAGAGCTGGAAGGCTCGCTTGTTCCCCAGGCGTCTGACGGCGGAAAACGATGACTCCTGAAAACAATTACAGTGAGTGAAGAATATGGGAATTTACAGGTTTCGGCAGCAAATCCCGTTTCTGAAGTCGATAGGCTGTTAATGCCCTAGCGGGTTCGGGTATAAGTTTACCATGGCTCCCGGCCGCAAACGA
GGAAAAGCTGTAGCTGCCCGCCAGGAATGGAAGGTTGGAGATCTCGTTCTAGCCAAGGTGAAAGGTTTTCCGGCCTGGCCCGCTCAGATAAGTACACCCGAAAAATGGGGACAGTCACACGACCCGAGAAAAGTATTTGTGTATTTTTTCGGCACAGATCAGATTGGGTTTTGCAGTCCTGATGCTATCCAGGCTTTTAC
ACAGGAGACCAAAAAGCATCTATTGTCGAAGACTCAAGGAAAGGGAACTGACTTTGTTCGTGCAGTGGAGCAGATTGTTGAGATCTATGAGAAGCTAAAGATGGAGAGGCAGGAAGACAGCCTAAATACTACTGTTGAAGGAGGTAAGCTTGATGGCCGAAGAGGATCTGCAAGAAAATTACAAGGTAAGAAAGGTAATC
GGAGTCGAGCATCACAATATCCACAGGAATTTCTTAATGTGGTGCCTGATGCTGAAAAGGAATCTTATATTCCGAGGGATCAAGCACTTGTTGCTAAAGGTAAAGATATTGAAAATCAAATTCAAGGGCTGGGAGATTTGCAAAAGATCACTGAGGTTGGCACTGATGGAACAACCAATAATGACCAAAACAATGAAATA
GCTTCTGTAAATAACTCTGTGAAGAAACGAGGAAGATTGCCAATTGAGAATCATGATATTCAGAAAAGGAAGCCCCCAAGAAGAAGTCCCAAATTTGAGACTGAGATATCAGAGAAAAGAAGGAATAATGATATTTGTGCACAGGCAACAAAAGTCAAGCAGGATATTAAGAAGAAAAAAAGAGGTGTAAAAAAGTCAGC
TGACAATAAGAACCTTTTTCAAGATGATGGAGACAAGGGTGCTATCATATCCCCAGGGAACCTTTTTCAAAATGACGGAGATGAGGGTGCTATCATTTCTCTAGGGCAAGATGAGAAGGCTTTCAGATTTCCAATTAGAACTTCTGATATAGATCTAAATAAAAGTGAAGTTGAGGTTCAAGAGAAGATTTCCAGCGAAG
CGGGTGCCCAGCTACAAGGGGAATCACTGAAAACAAATGATGCATCTGAAGCATTAAAATCTTTAGGTGATGGTCAGATGGAAGCTTTAAGAATTCCTACTAACCAGAATGTAATTTTATCAAATGAAAGTGTTGAATACAGAGAGGCATTGCAGGATATGCAGGTAAACTCAGCATCCCATGAAAAAAGCAGTGCCGCT
TCTCCATTTATAGATGAAGATGATGGAGACAGGGCAATCATAGATGATAGTGTACAGCATCACTATGTTTTAAAGAAGAAAAGGAAACACCTCAAGAAGTGTGATTTGGGACTATATGGGCCCACTAGGAGATTTATGGATTTTGGCAATGAAAGAGTTACTTCGACAGAGATTGAGGTAAGTGGTTTTCTGGCAAAATC
ACATGAGTTGGGTTCCAAAGCTAAGGGGGTTGAGCATAAACTTGACACTGATGAACATCTTCCTTTGGTAAAAAGGGTAAGAGCTCGTCTGAGAAAGGAGTCTAGTAAAACACAGGAAAATCTCAATGAAGATAACATGCCAAAGGCAGTGGAGGGCACTGCAGATCAGCATGACCTACAGGGTGCATTGCCATTGGATG
ATGCTAACTGCAAATCTAGTATTTTAATGGCTTCAGAGAATACTTATCCAGAAAGGGCATCTTCACATTTTGCGGATTCTGCTATGTTGGGTGAAAAAGATCCTTGTTTGCCACGAAAAGCTGATAAGTATCGTCTGAGGGGAAGTTTGCCAGATGATGAAGCTGCATTACCACCCTCTAAAAGAATTCATCGTGCTTTA
GAGGCAATGTCTGCATATGCTGCTGAAGCTGCTTCTGTTTCTGATGAAGTTGATGCTACAAGCATGGAAACACAAGATGATGGTAAAATTGTGTCTGCTTGTATGAGTAATGGCAATGAGGATGTTTCGAGGACGAAAGACCTTGCAAAAGGAGGAGATTTATTGGTTCCAAAAGATTCAACGGGAGAGACAAAGAATTC
AGAAATGTGCATAGATTCTTGTAAGTTAATTTCTGAAGTATCTTCAGAACCTTCTAATCTTTTAGAAGTAAAGGTGGGGAATGTTGCAGAAGCAGATACAAGGTCTGACATGCCTGGGGATCAAAGTAAAATTGACATCCAGGATGATAGGATAATCGATGCTAATAGTGCTGAAAAATCCATTGTCACTTCTCAGAAAA
TTGAAGAATCAAAGCAACATGAGAAGTCTTTGAAATTGTGTCCCAGAAAAAATGATGGTTTAGAATTTGACTTAGAGTGTACTGGTCAGACAGAAGGGAATACTTTTCTGGATACAAATTTAAGGTCTGATTTACAAGACAATCGAAATAAATTCATTGGTCAGGATGATGCTATAGGACATGCTAAGGATGCCAAAGAA
CTAGATTTCACCTCTCAAAAATGTGAAGAGTTAAAATTACGTGGCAAGTCCTTAGTAGTTTTTCCCAAGAAGCGTCATGGTTTGAAGTTGGCCTCAGAAAGTTTTAATCAAGTAGAAGAGAAGGCGGGGAATATTATGGATACAAATTTTAAGTCTCATGTTCATAATGATCAAATGGAGATGGATGGTCAAGGTCATGA
AATATTACATGCCAAGAATCCTAAAGTATCAAGTCTTAGTTCTCAGAAAACTGTCAAGTCAGAGCAACCTGCCATGCCTGAACTGGTGCTGCCTGTCAAATGTGATGGCTCAAAGTTTGCTTCAGAACACTATAATCAGGCAAAGGAGAATTTGGCAGGTCTTATGGACGTAGAACTCAAGTCTGATGTGCATGATGATC
AAAGGAAAATGGTCAATGAGGATGCTGCTGAATCAATTATTAGTTCTCAGAAAGCTGTCAAAGCAGAGCAACCTGGCATGCCTGTGAAACATGATGGTTCAAAGTTTGCTTCAGAACACTATAATCAGATCAAGGAGAATTTGGTGGGTCTTATGGATGAAGATCTCAAGTCTGTTGTGCAGGATCATCAAAGGAAAATG
AGCAATAAGGATCGTATAATAGTGCATGTCTTAGAATGTACTAGTCAGGCAGAAGAGAAGGCCTGTGAAATTATGGATACAGAGTTGAAGACTCATGTACAAGAGAATCAAATGATATTGATTAGTCAGGGTGACTTAAAAGGATATACTAAGGACACTGGACAATTAGATTTTACCTCTCAGGAACCTGACAAATTAAA
GCGACATGACAAGTCCATGACAGTATCTCCAAAGAAGCATCATGGTTTGAGTTCAGCCCTGGAAAGTTTTAAATGGCCAGGAGAGAAGATGGGGGATATTATAAATACGGAAAGTTTTAAATGGCCAGAAGAGAATATGGGGGATATTATAAATGCCAATTTGAAGTCTGATATTCATAACAATCAAAAGAAAATGCATG
GCCAGGGACATGCAATCTTAGATGCTACGACTGCTGAAGAATCAAGTCTTAGTCCTCAGCAAGATGTCGAAAGAAAGCATCATGCCATGCCTGTACGAATGCTGCCCAGGAAACATGATGGTGGGGATGGTATAGTAGTGCATGTCTTAGAATGTACTAGTCAGGCAGAAGAGAAGACCCATGGAATTATGGATACAGAT
TTGAAGACTCATGTTCAAGACAATCAAATGATATTGATTAGTCAGGATGACTTAGAAGGATATACTAAGGACACTGGAAATTTAGATTTTACCTCTCAGGAACCTGAAGAATTAAAGCGACATGGCAAGTCCATGACAGTATCTCCAAAGAAGCATCATGGTTTGAGTTCAGCCCTGGAAAGTTTTAAATGGCCAGGAGA
GAAGATGGGGGATATTATAAATACCAATTTGAAGTCTGATATTCATAACAATCAAAAGAAAATGGATGGCCAGGGTCATGCAATCTTAGATGCTAAGACTGCTGAAGAATCAAGTCTTAGTCCTCCGCAGGATGTCAAAACAAAGCAACATGGCATGCCTGTACGAATGCTGCCCCAGGAACATGATGGTTCAAAGTTTG
CTTCAGAGCACAATAATCAGGCAAAGGAGAACTTGGGTGGTCTTATGGATGTAAATCTCAAGTCTGATGCACATGATAATCAAAAGAAAATGGGCAATGGGGATGGTATAATACATTCCTTGGAATGTACGAGTCAGGTAGAAGAGAAGACATGCAATATTAACTGCTCCGATTCAAAGTACCATGTAGAAGACAATCAA
AAGGAATTCGTTGGTCAGGTTGATGTTATAGGATACGCTAGGGATAATGAAGAATTGGATTTCACCTCTCGAAAACCTGAAGAATTAAAGCACTGTGACATGTCCATGACTGTGTCTCCCAAGAAGCATTATTGTTTGAAGCTGGCCTCAGGAGCATTCAATCAGGCAGAAGAGAAGATGGGGGATATTATGGATGCAAA
TTTGAAGTCTCATAATGATCAAAAGAAAAAGGATGGTCAGGGTCATGTACTATTGCCTGTTAAGACTGCTGAAGAATCAAGTCTTAGCTCTCAGAAAGCTGTCAAATCAAAGCAGCATGCCATGCCTGTACCAATGCTGCCTGGGAAACATGATGGTTCAAAGTATGGCTCAGAACAGTATAATCAGGCAAAGGAGAATT
ATGGGGTTCTTACGGATACAGGTCTCAAGTCGGATTTGCATGATAATCAAAAGAAAATAGACAACGGGGATGGTATGACAGTGCATGATAAGAGTGGTAAAGAGTTTGTTGTTATCTGTCATAAAACTGATGAATCAAAGCAACATGTGAAGTCTGTTTCAGTGCCTCCAAGGAAACAGGATGGTTTGAAGTCTACTTTA
GACACTTCTAATCAGAAAAAGGAATGGGTGGGGTGTGTTATGCATGCAGATTTGAAGTCTCATGTGCATGATAATCAGAAGAAACTGGGCAGTGAGGATGTTACGATAGTGCATACTAAGAATGCAGAAGAACAGGTTGTTACCTCCCAGAAAACTGATGAATCAAAGCAACATGGCAAGCCTGTATTAGTGCCTCCCAG
GATGTATGACAATTTGAAACCTGATCTAAGGCAAGGCAGTTGTAGTTTATCTCCAACTAAGAGTTATGGCGTTTTAGATCGCGCGAAAGTTGATGCTCATCAATTAGAGCATAAGGCTAAGTTGATCAAAACTAGAAAATATAATTTAACGCCAAATAAAGAAATGTGTCTTGATGTGTCCAAAAAAGATGACGGGCAGT
TGCATGCAGAAAAACCAAATCAACAGCATCAGCATAGGCATCCAGTTGTTTGCACGCCAGAGAAGGTTTCACATGCCAAACCTGGACGTGATAATTCTACACCAAAGTCAAAAGTCATCTCAAAATGCAATGCTGATATAAATGGTACACTTATGGTTAGTAGGTCTGAGAGTGGAGCATGTTCTTCGAAGTTACATTTA
GATGAAGGCAACAAAATTAGGGAAGCGTATGAAGCTGCAAAAGAAGTAAAGCTCAAAATATCATCACAAAATTCTGGGCTGACTGCAGCATCTACATCTATGAAGTATCTAATTGCAGCTGCTCAGTCTAAACGACAGGCACGTTCTACTGTGGCACCTCCTCCTGTTGTGCCTGGAGAACTCGAAAAGTTTGCACCTGT
TCTAAGCCCTTCTCCAGCTCAAGGAAGAAGTTCTTGTCATCGTAGTTCACCCTCTCAGCCTTTACTGTATGGGAATTTGACAGTTGATGATACAAGAGATATGATGCTCCATGCTGAATCTAGGACCCCTGAAACAAGCACACAGCAGTGTGCTCCAACACAAATAACTGGTCCAGAAATGAGTGACGAGGGTAGAGCTA
GTTCTGGAAGAAAATCCGTTAGAGGGATACTTGATTCTGATGCAGAAGCAATTATGTCCAGGGATTCTTTCGAAGGAATGCTTGAAACATTGTCAAGGACGAGGGATAGTATCGGGAGAGCAACTCGCCTTGCTATTGAGTGTGCACAATATGGTATTGCAGGCGAGGTAGTGGAACTTCTTGTTCAGAAGTTGGAAAAT
GAACCGAGTTATCATCGAAGAGTAGACCTGTTCTTTTTGGTTGATTCTATCACACAATGTTCGCGTGGGCAGAAAGGTATTGCTGGAGATACGTATCCATCTGCTGTCCAATCTGCACTTCCGCGGCTGTTGAGTGCAGCTGCTCCTCCTGGAAGTCAAGCACGTGAAAATCGCAGACAATGTTTAAAGGTTTTGAGGCT
TTGGCTTGAGCGCAAAATTCTTTCAGAGTCTGTCATAAGGCGGCACATAAATGAGATTGAGGCCTCCATTGAGGATAGATCAAACACTGTCTTACCTAGGCGTCCATCTCGATTACAGCGTGCTCTTGATGATCCAATCAGAGAAATGGAAGGCATGCTTGATGAGTATGGCAGCAATACATGTTTTCAATTGCCTGGAT
TCATAATGCCACAAATGTCTGAAGATGAAGAAGAGCCAAATGCAGGTGATGGGAAGAAAGTTGAAGAAAATGTTCTGATTGAAGCAACTGAAGCCCTGGAAGAAGGAGCCAACAGCTATAATCCAGAAAGATATCGACACATTCTTGAGGATGTTGAAGGGGAGCTTGAAATGGAAGATGTATCCCCATCTTCAGAATAT
GGTGCAAGTTCTTGTAAGAATGATACTATTGGAGCAGATGCAAGACTAATTGGTCTCAGTGAAGCATTCTGTTCCCTAATGCCGAATGAAACAAATCAGTATCCTCCCTTGCCCTTGGATCCACCTCCACTACCAGAAGATATGCCCCCATCCCCTCCACCTCCACCTTCATCTCCACCACCTCCTTCACCACCTCCACC
ACCACCACCACCTCCACCTCCACCTCCACTTCCACTTTCTCCAGCCTATATCTCACGGCCTCTACTTTGTATACCCTATGCATCTCCAAATCTTTTGATGTTCGCAGGATGTGCAACATCACATTTTACCTGGAACGCCACAACAACCTCATCATGGAACTAACCATTCAGGAGGGTCAGATTCAATTTTGTACAGTTCT
CCTGCATACCCGATGTCTCAAAATCATAACTGCACAACTGCTAATGCCTTCAATTCTTCATCTTCTTATGGATTTTTACAGAGTAGTTTCCCTGGGGTTTCACATTGTGTACCCCTTGTCCAGCAAATGCATGGCAGTAATATGTCTTTTCCTCAGCAGTCTTACCGTCCACTTCCACCTACTCCTGCACCTTCCAATCA
GTTCTCATTTGTACGGGCAGAAGTGAGGCATCAAGTGCAACAGTTATGGCCAGATCCATCTTTATCCCCTGTACTGGAAAGTAAGCAACCGGTACAAGATGAACATAGGGGCCATCTGCATGGTGACTTTGGTAACATGAGATTAGAGGAGCAACAACCTATCCAAGGAAGAAATGAAATCATGGAAACCTTGCACTCAG
ATCATATTGTGTGGCCGAACCAAGGGATGCTCTTGTCTCCAACAGGAGCAGATAATGCTTGTGGTTTGTCTGGTTTTCATCCTCCACAACAGATCACAATGTCTCGTGGGTCTAGAAGTCCACTAGCAGCTAATCTCGTTCCAGCCCAGATAATACCATGCAATTCTGGCACTTCTACTCAAAATTGTTGGAGACCAAAC
TGATGTTCATGGTTGGAGCAAGCGAGCTGTGCCTTTGGGATGTATATTTCTCAATTGTGTTAGAGAAGATTTATGCTGTGGAAAACTCTTAAGGTTGACTACAAGTAAGTTACAAGGATTTCAATATGGCCGCTGGACAGGAAGACATTCTTAATAACTGTACATATTGTACTCTGTGCCTCTACCAGCATTTGCAGATG
CACTAAGGACATGTTTTCTGAAAGAGAGATATTATGACTTTCCGAAGGGTTTTACATGATTGAACCATTCATTATTGTAGGCTTATTTTCTATGTCCTGATCTTGTATTTTTTTATTATCAAATGACTATAAACAGGGCATATTGTCCAGAGTCAGAGAATGGATGGTGTACGCAATTAAAATTTTCTCAAAATCTTTTT
GTCAATGCCAAATTGAAGAATGTATTTAAACTGAGTTTAGTATTAACTTCTTCCTCCAAATCTGTTTAAAACCTAAAACATTTTGTTGCAAGTCGAATGTATCAAGTTACTAAAAATTAACCAGTTTATCTTCTGTCCTCAAGTGGCAATATAAGATAGAGCCACCAGGTTAGTGCCAGATTTGGATT

>TRINITY_DN114572_c1_g1_i5 from sample A2 / Example unspliced isoform:

AGTAGATCACACAGCAGTAGCACCAAAAGAAGAAGGCGGCATGGGCAAATTATCGAGTTCAAGTGGAACCGCCATGGCGGAGCCCCGTAAATTGAACCTTCGAGCTTCCAGGAAGCTAAAATCCTGCTGAATTACAGCCTCACTTGCCACTAAAAAGAACAAACGATACACACATAGTGTGAAAGACGTGTGGAGTTTGA
ACTGCTCGGAGAGCTGGAAGGCTCGCTTGTTCCCCAGGCGTCTGACGGCGGAAAACGATGACTCCTGAAAACAATTACAGTGAGTGAAGAATATGGGAATTTACAGGTTTCGGCAGCAAATCCCGTTTCTGAAGTCGATAGGCTGTTAATGCCCTAGCGGGTTCGGGTATAAGTTTACCATGGCTCCCGGCCGCAAACGA
GGAAAAGCTGTAGCTGCCCGCCAGGAATGGAAGGTTGGAGATCTCGTTCTAGCCAAGGTGAAAGGTTTTCCGGCCTGGCCCGCTCAGATAAGTACACCCGAAAAATGGGGACAGTCACACGACCCGAGAAAAGTATTTGTGTATTTTTTCGGCACAGATCAGATTGGGTTTTGCAGTCCTGATGCTATCCAGGCTTTTAC
ACAGGAGACCAAAAAGCATCTATTGTCGAAGACTCAAGGAAAGGGAACTGACTTTGTTCGTGCAGTGGAGCAGATTGTTGAGATCTATGAGAAGCTAAAGATGGAGAGGCAGGAAGACAGCCTAAATACTACTGTTGAAGGAGGTAAGCTTGATGGCCGAAGAGGATCTGCAAGAAAATTACAAGGTAAGAAAGGTAATC
GGAGTCGAGCATCACAATATCCACAGGAATTTCTTAATGTGGTGCCTGATGCTGAAAAGGAATCTTATATTCCGAGGGATCAAGCACTTGTTGCTAAAGGTAAAGATATTGAAAATCAAATTCAAGGGCTGGGAGATTTGCAAAAGATCACTGAGGTTGGCACTGATGGAACAACCAATAATGACCAAAACAATGAAATA
GCTTCTGTAAATAACTCTGTGAAGAAACGAGGAAGATTGCCAATTGAGAATCATGATATTCAGAAAAGGAAGCCCCCAAGAAGAAGTCCCAAATTTGAGACTGAGATATCAGAGAAAAGAAGGAATAATGATATTTGTGCACAGGCAACAAAAGTCAAGCAGGATATTAAGAAGAAAAAAAGAGGTGTAAAAAAGTCAGC
TGACAATAAGAACCTTTTTCAAGATGATGGAGACAAGGGTGCTATCATATCCCCAGGGAACCTTTTTCAAAATGACGGAGATGAGGGTGCTATCATTTCTCTAGGGCAAGATGAGAAGGCTTTCAGATTTCCAATTAGAACTTCTGATATAGATCTAAATAAAAGTGAAGTTGAGGTTCAAGAGAAGATTTCCAGCGAAG
CGGGTGCCCAGCTACAAGGGGAATCACTGAAAACAAATGATGCATCTGAAGCATTAAAATCTTTAGGTGATGGTCAGATGGAAGCTTTAAGAATTCCTACTAACCAGAATGTAATTTTATCAAATGAAAGTGTTGAATACAGAGAGGCATTGCAGGATATGCAGGTAAACTCAGCATCCCATGAAAAAAGCAGTGCCGCT
TCTCCATTTATAGATGAAGATGATGGAGACAGGGCAATCATAGATGATAGTGTACAGCATCACTATGTTTTAAAGAAGAAAAGGAAACACCTCAAGAAGTGTGATTTGGGACTATATGGGCCCACTAGGAGATTTATGGATTTTGGCAATGAAAGAGTTACTTCGACAGAGATTGAGGTAAGTGGTTTTCTGGCAAAATC
ACATGAGTTGGGTTCCAAAGCTAAGGGGGTTGAGCATAAACTTGACACTGATGAACATCTTCCTTTGGTAAAAAGGGTAAGAGCTCGTCTGAGAAAGGAGTCTAGTAAAACACAGGAAAATCTCAATGAAGATAACATGCCAAAGGCAGTGGAGGGCACTGCAGATCAGCATGACCTACAGGGTGCATTGCCATTGGATG
ATGCTAACTGCAAATCTAGTATTTTAATGGCTTCAGAGAATACTTATCCAGAAAGGGCATCTTCACATTTTGCGGATTCTGCTATGTTGGGTGAAAAAGATCCTTGTTTGCCACGAAAAGCTGATAAGTATCGTCTGAGGGGAAGTTTGCCAGATGATGAAGCTGCATTACCACCCTCTAAAAGAATTCATCGTGCTTTA
GAGGCAATGTCTGCATATGCTGCTGAAGCTGCTTCTGTTTCTGATGAAGTTGATGCTACAAGCATGGAAACACAAGATGATGGTAAAATTGTGTCTGCTTGTATGAGTAATGGCAATGAGGATGTTTCGAGGACAGAAGACCTTGCAAAAGGAGGAGATTTATTGGTTCCAAAAGATTCAACGGGAGAGACAAAGAATTC
AGAAATGTGCATAGATTCTTGTAAGTTAATTTCTGAAGTATCTTCAGAACCTTTGAATCTTTTAGAAGTAAAGGTGGGGAATGTTGCAGAAGCAGATACAAGGTCTGACATGCCTGGGGATCAAAGTAAAATTGACATCCAGGATGATAGGATAATCGATGCTAATAGTGCTGAAAAATCCATTGTCACTTCTCAGAAAA
TTGAAGAATCAAAGCAACATGAGAAGTCTTTGAAATTGTGTCCCAGAAAAAATGATGGTTTAGAATTTGACTTAGAGTGTACTGGTCAGACAGAAGGGAATACTTTTCTGGATACAAATTTAAGGTCTGATTTACAAGACAATCGAAATAAATTCATTGGTCAGGATGATGCTATAGGACATGCTAAGGATGCCAAAGAA
CTAGATTTCACCTCTCAAAAATGTGAAGAGTTAAAATTACGTGGCAAGTCCTTAGTAGTTTTTCCCAAGAAGCGTCATGGTTTGAAGTTGGCCTCAGAAAGTTTTAATCAAGTAGAAGAGAAGGCGGGGAATATTATGGATACAAATTTTAAGTCTCATGTTCATAATGATCAAATGGAGATGGATGGTCAAGGTCATGA
AATATTACATGCCAAGAATCCTAAAGTATCAAGTCTTAGTTCTCAGAAAACTGTCAAGTCAGAGCAACCTGCCATGCCTGAACTGGTGCTGCCTGTCAAATGTGATGGCTCAAAGTTTGCTTCAGAACACTATAATCAGGCAAAGGAGAATTTGGCAGGTCTTATGGACGTAGAACTCAAGTCTGATGTGCATGATGATC
AAAGGAAAATGGTCAATGAGGATGCTGCTGAATCAATTATTAGTTCTCAGAAAGCTGTCAAAGCAGAGCAACCTGGCATGCCTGTGAAACATGATGGTTCAAAGTTTGCTTCAGAACACTATAATCAGATCAAGGAGAATTTGGTGGGTCTTATGGATGAAGATCTCAAGTCTGTTGTGCAGGATCATCAAAGGAAAATG
AGCAATAAGGATCGTATAATAGTGCATGTCTTAGAATGTACTAGTCAGGCAGAAGAGAAGGCCTGTGAAATTATGGATACAGAGTTGAAGACTCATGTACAAGAGAATCAAATGATATTGATTAGTCAGGGTGACTTAAAAGGATATACTAAGGACACTGGACAATTAGATTTTACCTCTCAGGAACCTGACAAATTAAA
GCGACATGACAAGTCCATGACAGTATCTCCAAAGAAGCATCATGGTTTGAGTTCAGCCCTGGAAAGTTTTAAATGGCCAGGAGAGAAGATGGGGGATATTATAAATACGGAAAGTTTTAAATGGCCAGAAGAGAATATGGGGGATATTATAAATGCCAATTTGAAGTCTGATATTCATAACAATCAAAAGAAAATGCATG
GCCAGGGACATGCAATCTTAGATGCTACGACTGCTGAAGAATCAAGTCTTAGTCCTCAGCAAGATGTCGAAAGAAAGCATCATGCCATGCCTGTACGAATGCTGCCCAGGAAACATGATGGTGGGGATGGTATAGTAGTGCATGTCTTAGAATGTACTAGTCAGGCAGAAGAGAAGACCCATGGAATTATGGATACAGAT
TTGAAGACTCATGTTCAAGACAATCAAATGATATTGATTAGTCAGGATGACTTAGAAGGATATACTAAGGACACTGGAAATTTAGATTTTACCTCTCAGGAACCTGAAGAATTAAAGCGACATGGCAAGTCCATGACAGTATCTCCAAAGAAGCATCATGGTTTGAGTTCAGCCCTGGAAAGTTTTAAATGGCCAGGAGA
GAAGATGGGGGATATTATAAATACCAATTTGAAGTCTGATATTCATAACAATCAAAAGAAAATGGATGGCCAGGGTCATGCAATCTTAGATGCTAAGACTGCTGAAGAATCAAGTCTTAGTCCTCCGCAGGATGTCAAAACAAAGCAACATGGCATGCCTGTACGAATGCTGCCCCAGGAACATGATGGTTCAAAGTTTG
CTTCAGAGCACAATAATCAGGCAAAGGAGAACTTGGGTGGTCTTATGGATGTAAATCTCAAGTCTGATGCACATGATAATCAAAAGAAAATGGGCAATGGGGATGGTATAATACATTCCTTGGAATGTACGAGTCAGGTAGAAGAGAAGACATGCAATATTAACTGCTCCGATTCAAAGTACCATGTAGAAGACAATCAA
AAGGAATTCGTTGGTCAGGTTGATGTTATAGGATACGCTAGGGATAATGAAGAATTGGATTTCACCTCTCGAAAACCTGAAGAATTAAAGCACTGTGACATGTCCATGACTGTGTCTCCCAAGAAGCATTATTGTTTGAAGCTGGCCTCAGGAGCATTCAATCAGGCAGAAGAGAAGATGGGGGATATTATGGATGCAAA
TTTGAAGTCTCATAATGATCAAAAGAAAAAGGATGGTCAGGGTCATGTACTATTGCCTGTTAAGACTGCTGAAGAATCAAGTCTTAGCTCTCAGAAAGCTGTCAAATCAAAGCAGCATGCCATGCCTGTACCAATGCTGCCTGGGAAACATGATGGTTCAAAGTATGGCTCAGAACAGTATAATCAGGCAAAGGAGAATT
ATGGGGTTCTTACGGATACAGGTCTCAAGTCGGATTTGCATGATAATCAAAAGAAAATAGACAACGGGGATGGTATGACAGTGCATGATAAGAGTGGTAAAGAGTTTGTTGTTATCTGTCATAAAACTGATGAATCAAAGCAACATGTGAAGTCTGTTTCAGTGCCTCCAAGGAAACAGGATGGTTTGAAGTCTACTTTA
GACACTTCTAATCAGAAAAAGGAATGGGTGGGGTGTGTTATGCATGCAGATTTGAAGTCTCATGTGCATGATAATCAGAAGAAACTGGGCAGTGAGGATGTTACGATAGTGCATACTAAGAATGCAGAAGAACAGGTTGTTACCTCCCAGAAAACTGATGAATCAAAGCAACATGGCAAGCCTGTATTAGTGCCTCCCAG
GATGTATGACAATTTGAAACCTGATCTAAGGCAAGGCAGTTGTAGTTTATCTCCAACTAAGAGTTATGGCGTTTTAGATCGCGCGAAAGTTGATGCTCATCAATTAGAGCATAAGGCTAAGTTGATCAAAACTAGAAAATATAATTTAACGCCAAATAAAGAAATGTGTCTTGATGTGTCCAAAAAAGATGACGGGCAGT
TGCATGCAGAAAAACCAAATCAACAGCATCAGCATAGGCATCCAGTTGTTTGCACGCCAGAGAAGGTTTCACATGCCAAACCTGGACGTGATAATTCTACACCAAAGTCAAAAGTCATCTCAAAATGCAATGCTGATATAAATGGTACACTTATGGTTAGTAGGTCTGAGAGTGGAGCATGTTCTTCGAAGTTACATTTA
GATGAAGGCAACAAAATTAGGGAAGCgtaagtattgttataactgcatttgaattgagatgtttctgatagtttttatgtattccatcttatatgattttttatccagcataaccatgtgtatttgatgctcatcttggtctttggtggttcgtacactctcgttgtttttccagGTATGAAGCTGCAAAAGAAGTAAAG
CTCAAAATATCATCACAAAATTCTGGGCTGACTGCAGCATCTACATCTATGAAGTATCTAATTGCAGCTGCTCAGTCTAAACGACAGGCACGTTCTACTGTGGCACCTCCTCCTGTTGTGCCTGGAGAACTCGAAAAGTTTGCACCTGTTCTAAGCCCTTCTCCAGCTCAAGGAAGAAGTTCTTGTCATCGTAGTTCACC
CTCTCAGCCTTTACTGTATGGGAATTTGACAGTTGATGATACAAGAGATATGATGCTCCATGCTGAATCTAGGACCCCTGAAACAAGCACACAGCAGTGTGCTCCAACACAAATAACTGGTCCAGAAATGAGTGACGAGGGTAGAGCTAGTTCTGGAAGAAAATCCGTTAGAGGGATACTTGATTCTGATGCAGAAGCAA
TTATGTCCAGGGATTCTTTCGAAGGAATGCTTGAAACATTGTCAAGGACGAGGGATAGTATCGGGAGAGCAACTCGCCTTGCTATTGAGTGTGCACAATATGGTATTGCAGGCGAGGTAGTGGAACTTCTTGTTCAGAAGTTGGAAAATGAACCGAGTTATCATCGAAGAGTAGACCTGTTCTTTTTGGTTGATTCTATC
ACACAATGTTCGCGTGGGCAGAAAGGTATTGCTGGAGATACGTATCCATCTGCTGTCCAATCTGCACTTCCGCGGCTGTTGAGTGCAGCTGCTCCTCCTGGAAGTCAAGCACGTGAAAATCGCAGACAATGTTTAAAGGTTTTGAGGCTTTGGCTTGAGCGCAAAATTCTTTCAGAGTCTGTCATAAGGCGGCACATAAA
TGAGATTGAGGCCTCCATTGAGGATAGATCAAACACTGTCTTACCTAGGCGTCCATCTCGATTACAGCGTGCTCTTGATGATCCAATCAGAGAAATGGAAGGCATGCTTGATGAGTATGGCAGCAATACATGTTTTCAATTGCCTGGATTCATAATGCCACAAATGTCTGAAGATGAAGAAGAGCCAAATGCAGGTGATG
GGAAGAAAGTTGAAGAAAATGTTCTGATTGAAGCAACTGAAGCCCTGGAAGAAGGAGCCAACAGCTATAATCCAGAAAGATATCGACACATTCTTGAGGATGTTGAAGGGGAGCTTGAAATGGAAGATGTATCCCCATCTTCAGAATATGGTGCAAGTTCTTGTAAGAATGATACTATTGGAGCAGATGCAAGACTAATT
GGTCTCAGTGAAGCATTCTGTTCCCTAATGCCGAATGAAACAAATCAGTATCCTCCCTTGCCCTTGGATCCACCTCCACTACCAGAAGATATGCCCCCATCCCCTCCACCTCCACCTTCATCTCCACCACCTCCTTCACCACCTCCACCACCACCACCACCTCCACCTCCACCTCCACTTCCACTTTCTCCAGCCTATAT
CTCACGGCCTCTACTTTGTATACCCTATGCATCTCCAAAT

Intron 5 GTCAGATTCA ... ATTTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	63.16	7	5	19
A2	74.19	4	10	13
A3	100	0	5	8
A4	88.89	1	10	6
A5	63.64	2	2	5
A6	84.31	4	13	30
A7	93.33	3	43	41
A8	76.67	14	45	47
A9	79.55	9	40	30
A10	80	9	43	29

>TRINITY_DN118291_c0_g1_i2 from sample A1 / Example spliced isoform

TCCAGCCTATATCTCACGGCCTCTACTTTGTATACCCTATGCATCTCCAAATGATGTGCAACATCACATTTTACCTGGAACGCCACAACAACCTCATCATGGAACTAACCATTCAGGAGGAGTAGTTTCCCTGGGGTTTCACATTGTGTACCCCTTGTCCAGCAAATGCATGGCAGTAATATGTCTTTTCCTCAGCAGTC
TTACCGTCCACTTCCACCTACTCCTGCACCTTCCAATCAGTTCTCATTTGTACGGGCAGAAGTGAGGCATCAAGTGCAACAGTTATGGCCAGATCCATCTTTATCCCCTGTACTGGAAAGTAAGCAACCGGTACAAGATGAACATAGGGGCCATCTGCATGGTGACTTTGGTAACATGAGATTAGAGGAGCAACAACCTA
TCCAAGGAAGAAATGAAATCATGGAAACCTTGCACTCAGGTTAACTTTATCATTTTCCTATCATAGAACTTTTAAGACTTGAAGTCATTAATACTTGTTCATCTAGTTTTTGGAATGAAACCACGTTAAGTATTGGAGCATTCCAAAAACTCAATGGACAACACTACTAGCTGTGAGATTCTACACAATACAATCTTTAT
TACTTTCTCAGCTAGAGTTGTATTTCTACTCCTAGATTCACTATTCCGTGTTTCCCGTAGTAAAATACATGAAATGCTCTGCTTCTCTTTTGGGTACAGACTCTGTAGTTTTCAAGAAAACTAAAGCAGCTCATTTTCATA

>TRINITY_DN118291_c0_g1_i1 from sample A1 / Example unspliced isoform:

TCCAGCCTATATCTCACGGCCTCTACTTTGTATACCCTATGCATCTCCAAATGATGTGCAACATCACATTTTACCTGGAACGCCACAACAACCTCATCATGGAACTAACCATTCAGGAGGgtcagattcaattttgtacagttctcctgcatacccgatgtctcaaaatcataactgcacaactgctaatgccttcaatt
cttcatcttcttatggatttttacagAGTAGTTTCCCTGGGGTTTCACATTGTGTACCCCTTGTCCAGCAAATGCATGGCAGTAATATGTCTTTTCCTCAGCAGTCTTACCGTCCACTTCCACCTACTCCTGCACCTTCCAATCAGTTCTCATTTGTACGGGCAGAAGTGAGGCATCAAGTGCAACAGTTATGGCCAGAT
CCATCTTTATCCCCTGTACTGGAAAGTAAGCAACCGGTACAAGATGAACATAGGGGCCATCTGCATGGTGACTTTGGTAACATGAGATTAGAGGAGCAACAACCTATCCAAGGAAGAAATGAAATCATGGAAACCTTGCACTCAGATCATATTGTGTGGCCGAACCAAGGGATGCTCTTGTCTCCAACAGGAGCAGATAA
TGCTTGTGGTTTGTCTGGTTTTCATCCTCCACAACAGATCACAATGTCTCGTGGGTCTAGAAGTCCACTAGCAGCTAATCTCGTTCCAGCCCAGATAATACCATGCAATTCTGGCACTTCTACTCAAAATTGTTGGAGACCAAACTGATGTTCATGGTTGGAGCAAGCGAGCTGTGCCTTTGGGATGTATATTTCTCAAT
TGTGTTAGAGAAGATTTATGCTGTGGAAAACTCTTAAGGTTGACTACAAGTAAGTTACAAGGATTTCAATATGGCCGCTGGACAGGAAGACATTCTTAATAACTGTACATATTGTACTCTGTGCCTCTACCAGCATTTGCAGATGCACTAAGGACATGTTTTCTGAAAGAGAGATATTATGACTTTCCGAAGGGTTTTAC
ATGATTGAACCATTCATTATTGTAGGCTTATTTTCTATGTCCTGATCTTGTATTTTTTTATTATCAAATGACTATAAACAGGGCATATTGTCCAGAGTCAGAGAATGGATGGTGTACGCAATTAAAATTTTCTCAAAATCTTTTTGTCAATGCCAAATTGAAGAATGTATTTAAACTGAGTTTAGTATTAACTTCTTCCT
CCAAATCTGTTTAAAACCTAAAACATTTTGTTGCAAGTCGAATGTATCAAGTTACTAAAAATTAACCAGTTTATCTTCTGTCCTCAAGTGGCAATATAAGATAGAGCCACCAGGTTAGTGCCAGATTTGGATTGATATGGCTTTCACAATTAATATTTGGC