NA ----> NA

Intron 1 GTAGGCATTG ... CTGTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	41.03	19.5	4.3	13.3
A2	46.49	19.7	9.7	17
A3	43.96	12.3	4	9.7
A4	75	1	2.5	3
A5	45.47	15.7	2	13
A6	41.64	79.8	16.8	56.8
A7	31.23	110.2	15.7	49.8
A8	33.65	67.2	11.3	34
A9	36.09	100	42.5	56.2
A10	42.6	78	22.8	57.7

>TRINITY_DN42771_c0_g1_i1 from sample A6 / Example spliced isoform

CGGACTAGTTATGCGGGACATCAATTGGGATTAGTTATTTCTGTGTGCAGGACTTGTTTCCAAGAGGCTATAATTTATTTATTTTGTGATCGTGGCTCTTCCGACTGCCACACGCCACTGCTACGTCCTTCATAGTGCCATTCAATGTTTATGTTTTGCATGTATGGTAAAGAAGACAGTCTTACGAAACCACCGGGTTT
TCACGAGTGGCAGGAGGATTCCACAAAGCATAATGAATATATTTGGCCATCTTCGGCAATTTATCCCCGATTCACTACCGTCGTACGTTGTTCAGGGATCTGTAATCGACATCGAGAACCTGTAGATTCTGCTGATTTTTGCCCGTCCTGTCGACATTAATTGATCTGCTGCAGATCTTGATCAGCAAGCTGCCCGCTCA
GTTTAGGGTTACAGCGCTAGGGTTCTTCGTTGGATTCATTATTTTAAGATCGGTAGGGTTCGTAGCTACGAAATGCATTCTCAAGAGGCCTGAGAGGAGAGAATACATGAAACCTCCTTGCGGGGATTGCTCTTTCCTTGGCCGAGAGCAGCATGCAGTGGGCAGTTCGAACTTCGAACACAATGGAGTTCGATTTTTTT
TGTCATCAAGGTTTCTTGGAAGGAATTTCGAACGAGGAAACCGTGGGTCGAAAGACTCAGAGCTCTAACGTAACTGTAATGCATTCTTTTTTATTTTCTGCTCTGTAGATACATTTTCTTGGAGGTTTGGTTTGGATTATTCAGAAGCAAAAAGCAAGACACATATACGTACCAGGCATCCAGGGAAGGCCAGGCATTAT
TTTCTTTATTCATGTATTTCATATTTTTGCAGCAAATTTTGTTC

>TRINITY_DN42771_c0_g1_i3 from sample A6 / Example unspliced isoform:

ATCGTGGCTCTTCCGACTGCCACACGCCACTGCTACGTCCTTCATAGTGCCATTCAATGTTTATGTTTTGCATGTATGGTAAAGAAGACAGTCTTACGAAACCACCGGgtaggcattgcgattgacaacatttttgttctataaggtagaaaacaatactagtttgaaaagtgaatcataatttgtcataaaattcatgt
tgtattgcccagatctactgttttcagGTTTTCACGAGTGGCAGGAGGATTCCACAAAGCATAATGAATATATTTGGCCATCTTCGGCAATTTATCCCCGATTCACTACCGTCGTACGTTGTTCAGGGATCTGTAATCGACATCGAGAACCTGTAGATTCTGCTGATTTTTGCCCGTCCTGTCGACATTAATTGATCTGC
TGCAGATCTTGATCAGCAAGCTGCCCGCTCAGTTTAGGGTTACAGCGCTAGGGTTCTTCGTTGGATTCATTATTTTAAGATCGGTAGGGTTCGTAGCTACGAAATGCATTCTCAAGAGGCCTGAGAGGAGAGAATACATGAAACCTCCTTGCGGGGATTGCTCTTTCCTTGGCCGAGAGCAGCATGCAGTGGGCAGTTCG
AACTTCGAACACAATGGAGTTCGATTTTTTTTGTCATCAAGGTTTCTTGGAAGGAATTTCGAACGAGGAAACCGTGGGTCGAAAGACTCAGAGCTCTAACGTAACTGTAATGCATTCTTTTTTATTTTCTGCTCTGTAGATACATTTTCTTGGAGGTTTGGTTTGGATTATTCAGAAGCAAAAAGCAAGACACATATACG
TACCAGGCATCCAGGGAAGGCCAGGCATTATTTTCTTTATTCATGTATTTCATATTTTTGCAGCAAATTTTGTTC

Intron 2 GTAGGCATTG ... TATTGCCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.55	17	5.5	8.3
A2	40.92	15.8	10	12
A3	34.96	13	4.3	9.7
A4	78.57	0.8	2.7	3
A5	30	7	2	4
A6	29.24	70	18.2	39.8
A7	20.38	86	17.3	26.8
A8	25.87	47.8	12.5	21
A9	34.07	80.5	46.2	37.2
A10	34.32	62.5	24.8	40.7

>TRINITY_DN42771_c0_g1_i9 from sample A6 / Example spliced isoform

CGGACTAGTTATGCGGGACATCAATTGGGATTAGTTATTTCTGTGTGCAGGACTTGTTTCCAAGAGGCTATAATTTATTTATTTTGTGATCGTGGCTCTTCCGACTGCCACACGCCACTGCTACGTCCTTCATAGTGCCATTCAATGTTTATGTTTTGCATGTATGGTAAAGAAGACAGTCTTACGAAACCACCGGATCT
ACTGTTTTCAGGTTTTCACGAGTGGCAGGAGGATTCCACAAAGCATAATGAATATATTTGGCCATCTTCGGCAATTTATCCCCGATTCACTACCGTCGTACGTTGTTCAGGGATCTGTAATCGACATCGAGAACCTGTAGATTCTGCTGATTTTTGCCCGTCCTGTCGACATTAATTGATCTGCTGCAGATCTTGATCAG
CAAGCTGCCCGCTCAGTTTAGGGTTACAGCGCTAGGGTTCTTCGTTGGATTCATTATTTTAAGATCGGTAGGGTTCGTAGCTACGAAATGCATTCTCAAGAGGCCTGAGAGGAGAGAATACATGAAACCTCCTTGCGGGGATTGCTCTTTCCTTGGCCGAGAGCAGCATGCAGTGGGCAGTTCGAACTTCGAACACAATG
GAGTTCGATTTTTTTTGTCATCAAGGTTTCTTGGAAGGAATTTCGAACGAGGAAACCGTGGGTCGAAAGACTCAGAGCTCTAACGTAACTGTAATGCATTCTTTTTTATTTTCTGCTCTGTAGATACATTTTCTTGGAGGTTTGGTTTGGATTATTCAGAAGCAAAAAGCAAGACACATATACGTACCAGGCATCCAGGG
AAGGCCAGGCATTATTTTCTTTATTCATGTATTTCATATTTTTGCAGCAAATTTTGTTC

>TRINITY_DN42771_c0_g1_i3 from sample A6 / Example unspliced isoform:

ATCGTGGCTCTTCCGACTGCCACACGCCACTGCTACGTCCTTCATAGTGCCATTCAATGTTTATGTTTTGCATGTATGGTAAAGAAGACAGTCTTACGAAACCACCGGgtaggcattgcgattgacaacatttttgttctataaggtagaaaacaatactagtttgaaaagtgaatcataatttgtcataaaattcatgt
tgtattgcccagATCTACTGTTTTCAGGTTTTCACGAGTGGCAGGAGGATTCCACAAAGCATAATGAATATATTTGGCCATCTTCGGCAATTTATCCCCGATTCACTACCGTCGTACGTTGTTCAGGGATCTGTAATCGACATCGAGAACCTGTAGATTCTGCTGATTTTTGCCCGTCCTGTCGACATTAATTGATCTGC
TGCAGATCTTGATCAGCAAGCTGCCCGCTCAGTTTAGGGTTACAGCGCTAGGGTTCTTCGTTGGATTCATTATTTTAAGATCGGTAGGGTTCGTAGCTACGAAATGCATTCTCAAGAGGCCTGAGAGGAGAGAATACATGAAACCTCCTTGCGGGGATTGCTCTTTCCTTGGCCGAGAGCAGCATGCAGTGGGCAGTTCG
AACTTCGAACACAATGGAGTTCGATTTTTTTTGTCATCAAGGTTTCTTGGAAGGAATTTCGAACGAGGAAACCGTGGGTCGAAAGACTCAGAGCTCTAACGTAACTGTAATGCATTCTTTTTTATTTTCTGCTCTGTAGATACATTTTCTTGGAGGTTTGGTTTGGATTATTCAGAAGCAAAAAGCAAGACACATATACG
TACCAGGCATCCAGGGAAGGCCAGGCATTATTTTCTTTATTCATGTATTTCATATTTTTGCAGCAAATTTTGTTC

Intron 3 GTTATGCGGG ... GTGTGCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	54.84	14	0	17
A2	54.84	14	0	17
A3	63.64	16	1	28
A4	57.78	19	0	26
A5	50	6	0	6
A6	56.59	135	1	176
A7	58.62	108	2	153
A8	55.17	117	0	144
A9	56.55	126	8	164
A10	55.93	130	3	165

>TRINITY_DN44828_c0_g1_i1 from sample A8 / Example spliced isoform

CATCCACCGGTTCCGGCGACGGAAAAAATCGATGTCCAATCCCCCTGACATCCACCGGTTCCGGCGACGGAAAAAATCGATGTCCAATCCCCCTGACATCCACCGGTTCCGGCGACGGAAATTTTAAATTTTGAAATCCCGACTAGACTAGCTAATTCTGTTGCAGATCTTGATCAGCAAGCTGCCCGCTCAGTTTAGGG
TTACAGCGCTAGGGTTCTTGGTCGGATTCATTACTTTAAGATCGATAGGGTTCGTAACTACGAAATGCATTTTCAAGAGGCCTGAGAGGAGAGAATACATGAAACCTCCTTGCGGGGATTGCTCTTTCCTTGGCCGAGAGCAGCATGCAGTGGGCAGTTCGAACTTCGAACACAATGGCGTTCGATTTTTTTTGTCATCA
AGGTTTCTTGGAAGGAATTTCGAACGAGGAAACCGTGGGTGTTGTCGTCAAGGTTTCCTGGAAGGAATTTCGAACGAACGAGGAAACCGTGGGTCGAGAGACGAAGAGCTCTAACGTAACTGTAATACACTCTTTTTTATTTTCTGCTCTGTAGATACATTTTCTTGGAGGTTTGGTTTGGATTATTCGGAAGCAAAAAG
AAAGGCACATATACGTACCAGGCATCCAGGGAAGGCCAGGCATTATTTTCTTTATTCATGTATTTCATATTTTTGCAGCAAATTTTGTTCGTTATATTAATATCTCCGATCCATGGAAGTGCTGGAAAATTTTATACGTTTGTTGCCTAGATGATCAGTACCCAGTATTAATATAAAATTTGGCATCCTAAGCAGATGTT
CTTGCATTTCTTCTTATCTTGATTTACTTCTATGTCTAATGTTCTAAATTCATGTTACAGTGGAGAGTGATTAATTCTACCGAATAGGCATTATGAATTGTGCTTGAAATATGCACATGGGTATTCAGGACCTTTTGAAAACTTCTTGATGTAATTCAAACTGTTTATGAAAAGATAAACTCCAGGGCCGTATGAGTTTG
ACGGAAAATATCATCAGCGATTTTAAATATAGTAAGATGTGACAAGTTACAGACATACCTGCAATACCAAACGGCATTACCTAGTTCGCCTTAAACAAAGATAGATGTTCGTGCATTTTTTTTACTGAAATGTGTGCTAGGTTTTTATATTGCCTTGGGTTTTAAGCCGTAGAGACTGAAAGTCTTCGGTTATCTGGGTT
AACGTGGAATTAACGCGTGCAGTGGGCTCGTGCTTTGAAGGGATTTCAATATTTAAACCAGGGAGAAGCTCCTTGGAAACGCTTCCTTGAGTTTAATGTAATTAGATATTTTGTCCGTGTTAAAGCAAGATATATGGCTGTCAACGCCGGCGATTGCCAAAATGAAATTCACATAGTTTACTCAGTAGTTTGGAATTATG
GTCTGCTAAAATGATACAAACCAAAATCCAAGAAGTTTTGAAGTTAAAAAGAAATCAATTAACAAACCAAACACTACTTAGGATATCGATGAAAGAAGTTGACAACCAGCTTCATGAATGAGGTCCTTGGTCGAGGTTACAATCAATACAACCGTTGTTTTCTCTTGTCTCGATTCATACGTTATCTTGTTATTGGGAAG
AGGAGCCTAGCTTCTCGCTCTGGGCTATGGTGAAATTGTGCAGGGTTGTGTTACAAATGAGATAGGGGCTCTCTGTGTTGGTTTTTATTAGTACTACAAATATGTTCGTTTCTGTATTGATTTCTTATTATATGGTTGAGGTGTTCCTTTCAAAGTCAGTATGTGGTTGATGGTTTGGAAT

>TRINITY_DN44828_c0_g1_i3 from sample A8 / Example unspliced isoform:

CATCCACCGGTTCCGGCGACGGAAAAAATCGATGTCCAATCCCCCTGACATCCACCGGTTCCGGCGACGGAAAAAATCGATGTCCAATCCCCCTGACATCCACCGGTTCCGGCGACGGAAATTTTAAATTTTGAAATCCCGACTAgttatgcgggacattaattgggactagttatttctgtgtgcatgaggacttgttt
cgaagaggctataatttatttattttgtgtgcgcagGACTAGCTAATTCTGTTGCAGATCTTGATCAGCAAGCTGCCCGCTCAGTTTAGGGTTACAGCGCTAGGGTTCTTGGTCGGATTCATTACTTTAAGATCGATAGGGTTCGTAACTACGAAATGCATTTTCAAGAGGCCTGAGAGGAGAGAATACATGAAACCTCC
TTGCGGGGATTGCTCTTTCCTTGGCCGAGAGCAGCATGCAGTGGGCAGTTCGAACTTCGAACACAATGGCGTTCGATTTTTTTTGTCATCAAGGTTTCTTGGAAGGAATTTCGAACGAGGAAACCGTGGGTGTTGTCGTCAAGGTTTCCTGGAAGGAATTTCGAACGAACGAGGAAACCGTGGGTCGAGAGACGAAGAGC
TCTAACGTAACTGTAATACACTCTTTTTTATTTTCTGCTCTGTAGATACATTTTCTTGGAGGTTTGGTTTGGATTATTCGGAAGCAAAAAGAAAGGCACATATACGTACCAGGCATCCAGGGAAGGCCAGGCATTATTTTCTTTATTCATGTATTTCATATTTTTGCAGCAAATTTTGTTCGTTATATTAATATCTCCGA
TCCATGGAAGTGCTGGAAAATTTTATACGTTTGTTGCCTAGATGATCAGTACCCAGTATTAATATAAAATTTGGCATCCTAAGCAGATGTTCTTGCATTTCTTCTTATCTTGATTTACTTCTATGTCTAATGTTCTAAATTCATGTTACAGTGGAGAGTGATTAATTCTACCGAATAGGCATTATGAATTGTGCTTGAAA
TATGCACATGGGTATTCAGGACCTTTTGAAAACTTCTTGATGTAATTCAAACTGTTTATGAAAAGATAAACTCCAGGGCCGTATGAGTTTGACGGAAAATATCATCAGCGATTTTAAATATAGTAAGATGTGACAAGTTACAGACATACCTGCAATACCAAACGGCATTACCTAGTTCGCCTTAAACAAAGATAGATGTT
CGTGCATTTTTTTTACTGAAATGTGTGCTAGGTTTTTATATTGCCTTGGGTTTTAAGCCGTAGAGACTGAAAGTCTTCGGTTATCTGGGTTAACGTGGAATTAACGCGTGCAGTGGGCTCGTGCTTTGAAGGGATTTCAATATTTAAACCAGGGAGAAGCTCCTTGGAAACGCTTCCTTGAGTTTAATGTAATTAGATAT
TTTGTCCGTGTTAAAGCAAGATATATGGCTGTCAACGCCGGCGATTGCCAAAATGAAATTCACATAGTTTACTCAGTAGTTTGGAATTATGGTCTGCTAAAATGATACAAACCAAAATCCAAGAAGTTTTGAAGTTAAAAAGAAATCAATTAACAAACCAAACACTACTTAGGATATCGATGAAAGAAGTTGACAACCAG
CTTCATGAATGAGGTCCTTGGTCGAGGTTACAATCAATACAACCGTTGTTTTCTCTTGTCTCGATTCATACGTTATCTTGTTATTGCGAAGAGAAGCCTAGCTTGTGGCTTTGGGTTGTTGTGAAATTGTGCAGGGTTGTGTTACAAATGAGATAGGGGCTCTCTGTGTTGGTTTTTATTAGTACTACAAATATGTTCGT
TTCTGTATTGATTTCTTATTATATGGTTGAGGTGTTCCTTTCAAAGTCAGTATGTGGTTGATGGTTTGGAAT

Intron 4 GTAAAGTACA ... ATTTATATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	93.75	1	0	15
A2	78.38	8	0	29
A3	96.55	1	0	28
A4	76.67	7	0	23
A5	66.67	7	0	14
A6	76.6	62	1	203
A7	73.3	51	6	140
A8	76.15	31	7	99
A9	79.55	54	7	210
A10	81.09	52	2	223

>TRINITY_DN47259_c0_g1_i8 from sample A7 / Example spliced isoform

TTAAATTACTCCGACAAAGCATGGCCGCGGCGGCACGTTGTCGGTATGTCGGACCACATCATCGATTAAGCCGCCCCGTCGTGCCTTCCCGAGCCAGAAAACGCGTATATTCAATCAGGTCATTAGTTGTAGCCAATGATGGCGTTGAAGCCATGTACTGGGACTAATTTGGGAGTCTCTCAAGATGCCCATAAAGGTCG
CCTGCTTTCCTGGATCTGTTTTGCGGTCCTTTGTCTCGGATGGATAGATAAATGTTCTGTAAACGCGTGCAGCTTTCACAGGTTCTGGCTCTGCTCTGATCATTCAGTTTATCCCCGATTCACTAACGTCGTACGTTGTTCAGGGATCTGCAGATTCTGCTGTTTTTTGCCGTCCTGTCAACACTAATTCTGTTGCAGAT
CTTGATCAGCAAGCTGCCCGCTCAGTTTAGGGTTACAGCGCTAGGGTTCTTCCTAGGGTTCTTCGTCGGATTCATTCTTTAAAGATCGGTAGGGTTCGTACCTACGAAATGCATTCTCAAGAGGCCTGAGAGGAGAGAATACATGAAACCTCCTTGCGGGGATTTGCTCTTTCCTTGGCCGAGAGCAGCATGCAGTGGGC
AGTTCGAACTTCGAACACAATGGCGTTCGATTTTTTTTGTCATCAAGGTTTCTTGGAAGGAATTTCGAACGAGGAAACCGTGGGTCGAAAGATGGAGAGCTCTAACGTAACTGCAATGCATTCTTTTTTATTTTCTGCTCTGTAGATACATATTCATGGAGGTTTGGTTTGGATTATTCAGAAGAAAAATACAAGACACA
AATACGTACCAGGCATCCAGGGAAGGCCAGGCATTATTTTCTTTATTCATGTATTTCATATTTTTGCAGCAAATTTTGTTCGTTATATTAATATCTCCGATCCATGGAAGTGCTGGAAAATTTTATACGTTTGTTGCCGAGATGATCAGTACCCAGTATTAATATAAAATTTGGCATCCTAAGCAGATGTTCTTGCATTT
CTTCTTATCTTGATTTACTTCTATGTCTAATGTTCTAAATTCATGTTACAGTGGAGAGTGATTAATTCTACCGAATAGGCATTATGAATTGTGCTTGAAATATGCACATGGGTATTCAGGACCTTTTGAAAACTTCTTGATGTAATTCAAACTGTTTATGAAAAGATAAACTCCAGGGCCGTATGAGTTTGACGGAAAAT
ATCATCAGCGATTTTAAATATAGTAAGATGTGACAAGTTACAGACATACCTGCAATACCAAACGGCATTACCTAGTTCGCCTTAAACAAAGATAGATGTTCGTGCATTTTTTTTACTGAAATGTGTGCTAGGTTTTTATATTGCCTTGGGTTTTAAGCCGTAGAGACTGAAAGTCTTCGGTTATCTGGGTTAACGTGGAA
TTAACGCGTGCAGTGGGCTCGTGCTTTGAAGGGATTTCAATATTTAAACCAGGGAGAAGCTCCTTGGAAACGCTTCCTTGAGTTTAATGTAATTAGATATTTTGTCCGTGTTAAAGCAAGATATATGGCTGTCAACGCCGGCGATTGCCAAAATGAAATTCACATAGTTTACTCA

>TRINITY_DN47259_c0_g1_i14 from sample A7 / Example unspliced isoform:

TTAAATTACTCCGACAAAGCATGGCCGCGGCGGCACGTTGTCGGTATGTCGGACCACATCATCGATTAAGCCGCCCCGTCGTGCCTTCCCGAGCCAGAAAACGCGTATATTCAATCAGgtaaagtacaaaaccctaactagtaacgagtagcattcatattatgttgacaagtttgttccaacggatagactgcggcgtt
cagcagactgctgttctttccagattctcacgctgctatatttatatagGTCATTAGTTGTAGCCAATGATGGCGTTGAAGCCATGTACTGGGACTAATTTGGGAGTCTCTCAAGATGCCCATAAAGGTCGCCTGCTTTCCTGGATCTGTTTTGCGGTCCTTTGTCTCGGATGGATAGATAAATGTTCTGTAAACGCGTG
CAGCTTTCACAGGTTCTGGCTCTGCTCTGATCATTCAGTTTATCCCCGATTCACTAACGTCGTACGTTGTTCAGGGATCTGCAGATTCTGCTGTTTTTTGCCGTCCTGTCAACACTAATTCTGTTGCAGATCTTGATCAGCAAGCTGCCCGCTCAGTTTAGGGTTACAGCGCTAGGGTTCTTCCTAGGGTTCTTCGTCGG
ATTCATTCTTTAAAGATCGGTAGGGTTCGTACCTACGAAATGCATTCTCAAGAGGCCTGAGAGGAGAGAATACATGAAACCTCCTTGCGGGGATTTGCTCTTTCCTTGGCCGAGAGCAGCATGCAGTGGGCAGTTCGAACTTCGAACACAATGGCGTTCGATTTTTTTTGTCATCAAGGTTTCTTGGAAGGAATTTCGAA
CGAGGAAACCGTGGGTCGAAAGATGGAGAGCTCTAACGTAACTGCAATGCATTCTTTTTTATTTTCTGCTCTGTAGATACATATTCATGGAGGTTTGGTTTGGATTATTCAGAAGAAAAATACAAGACACAAATACGTACCAGGCATCCAGGGAAGGCCAGGCATTATTTTCTTTATTCATGTATTTCATATTTTTGCAG
CAAATTTTGTTCGTTATATTAATATCTCCGATCCATGGAAGTGCTGGAAAATTTTATACGTTTGTTGCCGAGATGATCAGTACCCAGTATTAATATAAAATTTGGCATCCTAAGCAGATGTTCTTGCATTTCTTCTTATCTTGATTTACTTCTATGTCTAATGTTCTAAATTCATGTTACAGTGGAGAGTGATTAATTCT
ACCGAATAGGCATTATGAATTGTGCTTGAAATATGCACATGGGTATTCAGGACCTTTTGAAAACTTCTTGATGTAATTCAAACTGTTTATGAAAAGATAAACTCCAGGGCCGTATGAGTTTGACGGAAAATATCATCAGCGATTTTAAATATAGTAAGATGTGACAAGTTACAGACATACCTGCAATACCAAACGGCATT
ACCTAGTTCGCCTTAAACAAAGATAGATGTTCGTGCATTTTTTTTACTGAAATGTGTGCTAGGTTTTTATATTGCCTTGGGTTTTAAGCCGTAGAGACTGAAAGTCTTCGGTTATCTGGGTTAACGTGGAATTAACGCGTGCAGTGGGCTCGTGCTTTGAAGGGATTTCAATATTTAAACCAGGGAGAAGCTCCTTGGAA
ACGCTTCCTTGAGTTTAATGTAATTAGATATTTTGTCCGTGTTAAAGCAAGATATATGGCTGTCAACGCCGGCGATTGCCAAAATGAAATTCACATAGTTTACTCA