sp|Q5K5B6|RH57_ORYSJ ----> DEAD-box ATP-dependent RNA helicase 57

Intron 1 GTGAGTTACA ... TTTTAACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	84.62	5	21	34
A2	100	0	14	25
A3	85.37	3	6	29
A4	60	8	7	17
A5	81.82	2	7	11
A6	65.71	6	8	15
A7	54.39	13	7	24
A8	63.33	11	4	34
A9	72.09	6	13	18
A10	55.56	6	3	12

>TRINITY_DN102211_c1_g1_i7 from sample A4 / Example spliced isoform

CGAATAATTGTTGGTAGAAAGGGGTATATCTTATTCTGTAAAAAGCATCATAGTTGATACAACAGAAATTTTGTTGGAAATGAGGAAGGAAAGCTGCTTGCTCTTCGTCAGATTTTTGTGGAGGTTAAAGTGGAAGTAAAGGTGTTGGTTCATAACTTCATTGTAGTGTAGAGAAAGGCCTGGTTGACGAAGTTATTGCT
TTGGTGGTTTCCTTTCTCATCCTCGGAGCTTCAACCCATCAAGTGAGAAGGTACGGAATGTGATTGGAGACAGAGGGC

>TRINITY_DN102211_c1_g1_i1 from sample A4 / Example unspliced isoform:

CGAGTCCCTCATTTGTACGTTCATTATTTAGTGCTACGTTGCATGATTCCATTGAGGAGATTTCTCGCACCATCATGCATGATGCAGTACGAATAATTGTTGGTAGAAAgtgagttacataaaattcattttttcctacgctataccttgcatttctatattttgatatttactaggattcttcatgtcatgataggatc
aacttttgaatgcatttaatattctatgtattacacagatttccaagtatctgcactattttaaccagGGGGTATATCTTATTCTGTAAAAAGCATCATAGTTGATACAACAGAAATTTTGTTGGAAATGAGGAAGGAAAGCTGCTTGCTCTTCGTCAGATTTTTGTGGAGGTTAAAGTGGAAGTAAAGGTGTTGGTTCA
TAACTTCATTGTAGTGTAGAGAAAGGCCTGGTTGACGAAGTTATTGCTTTGGTGGTTTCCTTTCTCATCCTCGGAGCTTCAACCCATCAAGTGAGAAGGTACGGAATGTGATTGGAGACAGAGGGC

Intron 2 GTGAGTTACA ... TATTACACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	34.67	49	21	31
A2	43.43	28	14	29
A3	39.53	26	6	28
A4	45.45	12	7	13
A5	40.54	11	7	8
A6	20.99	32	8	9
A7	25.3	31	7	14
A8	23.08	45	4	23
A9	26.42	39	13	15
A10	17.65	21	3	6

>TRINITY_DN102211_c1_g1_i9 from sample A4 / Example spliced isoform

CGAGTCCCTCATTTGTACGTTCATTATTTAGTGCTACGTTGCATGATTCCATTGAGGAGATTTCTCGCACCATCATGCATGATGCAGTACGAATAATTGTTGGTAGAAAATTTCCAAGTATCTGCACTATTTTAACCAGGGGGTATATCTTATTCTGTAAAAAGCATCATAGTTGATACAACAGAAATTTTGTTGGAAAT
GAGGAAGGAAAGCTGCTTGCTCTTCGTCAGATTTTTGTGGAGGTTAAAGTGGAAGTAAAGGTGTTGGTTCATAACTTCATTGTAGTGTAGAGAAAGGCCTGGTTGACGAAGTTATTGCTTTGGTGGTTTCCTTTCTCATCCTCGGAGCTTCAACCCATCAAGTGAGAAGGTACGGAATGTGATTGGAGACAGAGGGC

>TRINITY_DN102211_c1_g1_i1 from sample A4 / Example unspliced isoform:

CGAGTCCCTCATTTGTACGTTCATTATTTAGTGCTACGTTGCATGATTCCATTGAGGAGATTTCTCGCACCATCATGCATGATGCAGTACGAATAATTGTTGGTAGAAAgtgagttacataaaattcattttttcctacgctataccttgcatttctatattttgatatttactaggattcttcatgtcatgataggatc
aacttttgaatgcatttaatattctatgtattacacagATTTCCAAGTATCTGCACTATTTTAACCAGGGGGTATATCTTATTCTGTAAAAAGCATCATAGTTGATACAACAGAAATTTTGTTGGAAATGAGGAAGGAAAGCTGCTTGCTCTTCGTCAGATTTTTGTGGAGGTTAAAGTGGAAGTAAAGGTGTTGGTTCA
TAACTTCATTGTAGTGTAGAGAAAGGCCTGGTTGACGAAGTTATTGCTTTGGTGGTTTCCTTTCTCATCCTCGGAGCTTCAACCCATCAAGTGAGAAGGTACGGAATGTGATTGGAGACAGAGGGC

Intron 3 GTAATTTTAA ... GTGATGGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.14	186	87	0
A2	34.54	177	93	2
A3	29.25	215.7	89	0
A4	31.18	179	81	1
A5	29.32	111	46	0
A6	32.07	1287	604	0
A7	31.73	1140.7	528	2
A8	30.92	1014	450	0
A9	30.98	1157.7	517	1
A10	32.56	988.7	476	0

>TRINITY_DN121522_c0_g1_i7 from sample A2 / Example spliced isoform

CCTTCTCAAAGCTGTGTTCGTTCTTCTCCGGTTGATTATTTCATTTCTGGCATTCGAGTTCCGGCTCTATATTGTTTTGCCCTCGGGTCCGAGAGATTTGCTGTATTATCTTCTGCGGTCTGTAAGGGAATGACTCCTCGCCATCGCTGGCACTCCACGAAGGGTTTTCTTTAAGCTCCAAGGAAGCAGCCAATACACTT
CGATGCCTCGGAGGTGCTTCCAGCTTGGAATGACGCAGATTTAGAGTGAGTCTTCCCTGACCGGTGAATTTGAAAGTGAAGGTAGTTTCGGTTGCATTACCTATCACTGCAACTGCTCAAGCGAAAAATCCG

>TRINITY_DN121522_c0_g1_i5 from sample A2 / Example unspliced isoform:

CCTTCTCAAAGCTGTGTTCGTTCTTCTCCGGTTGATTATTTCATTTCTGGCATTCGAGTTCCGGCTCTATATTGTTTTGCCCTCGGGTCCGAGAGATTTGCTGTATTATCTTCTGCGGTCTGTAAGGGAATGACTCCTCGCCATCGCTGGCACTCCACGAAGGGTTTTCTTTAAGCTCCAAGGAAGCAGCCAATACACTT
CGATGCCTCGGAGgtaattttaaaatgccaagttcagaatttctttttgttgaagtggttcaaccttaatgggaaataacgtattgtctgttgtgatggtagGTGCTTCCAGCTTGG