sp|Q76CU2|PDR1_TOBAC ----> Pleiotropic drug resistance protein 1


Intron 1 GTAAACCCCT ... TGTTATGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A118.6222529.473.6
A216.9213530.624.4
A319.817728.259.2
A420.231252934.4
A519.38842119.4
A618.1835174.8194.2
A717.69923195.6201.2
A818.21870174.8212.6
A918.24833174.4197.4
A1018.51596147123.8



>TRINITY_DN45077_c1_g1_i1 from sample A6 / Example spliced isoform
GAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAG
AGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGG
AAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCAC
ATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCA
CATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTA
TTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTT
CCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACAC
TTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTC
GCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGGCATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAAT
GAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGTTAGGGCAAGTTCACGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCTGGAAAGAAAGGAATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACAT
GCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCA
AGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATACTGAGTTAAGAAAGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTC
GGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCTGCTGCTATTGTGATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACC
CAGCATTGATATCTTCGAGGCTTTTGATGAGCTTGTGTTGATGAAGCGAGGAGGACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGCCATTCCAGGCGTTCCCAAAATCACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGC
GTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAAATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAACCGAATTTTCACAATCCTTTGCAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTAC
AATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATCTTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGATGTTTGCGGCGGTGCTATTCATTGGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAG
AGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTACTGATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGATCGACTTCGAGTGGAAGGCAACCAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAA
TGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATTGTGTCCTCGGCTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGAAAATTCCGGTATGGTGGAGATGGTATTACTGGGCATGCCCTGTTGCATGGACTCTGTACGGTTTGATTGCATCACAGTTCGGGGATTATGTTACG
CCCATGAATAGAACAGACGGAACTACACAGCCATTGAATGAGTTTCTCAGAAGTTATTTTGGATTCAAACATAGCTTTTTGGGACCTGTTGCAGTTGTGACAGCAGGATGGAGCGTTCTCTTCGCATTCATCTTCGCCTTCTCCATCAAGAAGCTCAACTTCCAGTCAAGATAATTACTGG


>TRINITY_DN45077_c1_g1_i2 from sample A6 / Example unspliced isoform:
GAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAG
AGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGG
AAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCAC
ATTATTTATCAGGGTCAGCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCA
CATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTA
TTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTT
CCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACAC
TTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTC
GCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGGCATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAAT
GAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGTTAGGGCAAGTTCACGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCTGGAAAGAAAGGAATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACAT
GCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCA
AGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATACTGAGTTAAGAAAGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTC
GGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCTGCTGCTATTGTGATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACC
CAGCATTGATATCTTCGAGGCTTTTGATGAGCTTGTGTTGATGAAGCGAGGAGGACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGCCATTCCAGGCGTTCCCAAAATCACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGC
GTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAAATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAACCGAATTTTCACAATCCTTTGCAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTAC
AATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATCTTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGATGTTTGCGGCGGTGCTATTCATTGGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAG
AGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGgtaaacccctaatatcatccccccattaacgcctataacttttttacttcttctggtttttaatttaacaccacccatcaatgccaataagctttttcaaaccttttttcatcactaattggaatgtggtcatggttcaataatgatgtta
tgcagGTACTGATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGATCGACTTCGAGTGGAAGGCAACCAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATT
GTGTCCTCGGCTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGAAAATTCCGGTATGGTGGAGATGGTATTACTGGGCATGCCCTGTTGCATGGACTCTGTACGGTTTGATTGCATCACAGTTCGGGGATTATGTTACGCCCATGAATAGAACAGACGGAACTACACAGCCATTGAATGAGTT
TCTCAGAAGTTATTTTGGATTCAAACATAGCTTTTTGGGACCTGTTGCAGTTGTGACAGCAGGATGGAGCGTTCTCTTCGCATTCATCTTCGCCTTCTCCATCAAGAAGCTCAACTTCCAGTCAAGATAATTACTGG



Intron 2 GTATGTTAAT ... CACTTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A125.11167056
A222.7126037
A321.36232063
A424.02174055
A530.67113650
A624.13283090
A723.973520111
A818.8190044
A921.333910106
A1021.674230117



>TRINITY_DN112159_c0_g1_i8 from sample A5 / Example spliced isoform
CAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTTATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCCTACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAACAACCCATTAAGCTTCT
GATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGTTCGTCCATCACCGAGC
ATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTTTCTAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCTTGCAAATGTACATGAC
AAAGGGGTTATCGATCATGATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATTTGAACATCGATGCGAA
TGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGCCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCA
TGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTA
TTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAA
GAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCA
TACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCA
GATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCA
GATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACC
CAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAATTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGC
AATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGG
CGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGGCATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTG
TCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGTCAGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCTGGAAAGAAAGGAATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTT
GTAGACATGCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTTTAATGGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCTATCACAATCTCCGG
TTATCCCAAGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATACTGAGTTAAGAAAGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAG
CTCTGGTCGGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCTGCTGCTATTGTGATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATT
CATCAACCCAGCATTGATATCTTCGAGGCTTTTGACGAGCTTGTGTTGATGAAGCGAGGAGGACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGCCATTCCAGGCGTTCCCAAAATCACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGC
AGAATTGCGTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAAATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAACCGAATTTTCACAATCCTTTGCAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATC
CCGAGTACAATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATCTTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGATGTTTGCGGCGGTGCTATTCATTGGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTT
TTCTACAGAGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTACTGATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGATCGACTTCGAGTGGAAGGCAACCAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATA
CTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATTGTGTCCTCGGCTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGAAAATTCCGGTATGGTGGAGATGGTATTACTGGGCATGCCCTGTTGCATGGACTCTGTACGGTTTGATTGCATCACAGTTCGGGGATT
ATGTTACGCCCATGAATAGAACAGACGGAACTACACAGCCATTGAATGAGTTTCTCAGAAGTTATTTTGGATTCAAACATAGCTTTTTGGGACCTGTTGCAGTTATGACAGCAGGATGGAGCGTTCTCTTCGCATTCATCTTCGCCTTCTCCATCAAGAAGCTCAACTTCCAGTCAAGATAATTACTGGAATATCAGTTA
TTATTTTTCCAATGTGTCATTACCAGTGACAATTTACTGTCATATATAAATTACATTAGCGATTCATTGTTTCTGCCACTTTAGACTATTGTATTTATCGATAGGTTTTCTTTTCTCAAAGGATATGCTTCGTCTCCCTCGGAACCTCTGTAACTGTAGGGGGGCAGCATACTGTAAAATCCCATGTCATCAATTTTCAA
TGAGAAGACGGCCCACTGGTTACCTAAGTGCACTTTTTTATTTGGATTCGGTTGTTAATTATTTAGCTAATTAATTTCATGACAATAACATGGCTTA


>TRINITY_DN112159_c0_g1_i11 from sample A5 / Example unspliced isoform:
TTATTTTGTAGACATGCCCCCGgtatgttaatcatcccatctctttgaattaattactgtaaacaaatattgtaaatcctttttgtcctccattgtaatggtgttataatttgattttaaagcttgtcagtaattaataatggtgtacactttgcagGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTAT
TGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTTTAATGGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTC



Intron 3 GTAGTGTTTG ... TGATTTTCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A118.11199044
A220.14115129
A314.54194233
A416.67155031
A58.919209
A617.862305
A714.5882014
A801600
A99.097007
A1014.73110019



>TRINITY_DN121424_c0_g1_i4 from sample A3 / Example spliced isoform
CTGTCCTCTCGTCCTATGTGTAGGTAGTTACGGAGTACACGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAA
TGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTAATCTTAAACTCCCAACCAGCAATGAAGAACTGAGAGGCCCAACGGCGATAAAAAAATTGATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCC
TACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAACAACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTG
ATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGTTCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTTTCTAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAA
AAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCTTGCAAATGTACATGACAAAGGGGTTATCGATCATGATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGG
CATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATTTGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGCCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCG
GCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGA
GAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATG
CGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGG
TGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAG
TACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAG
AGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGA
TGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTC
ACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAAT
TGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGG
CATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGTCAGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCTGGAAAGAAAGGA
ATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACATGCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGT
TCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCAAGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATACTGAGTTAAGAA
AGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTCGGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCTGCTGCTATTGTG
ATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACCCAGCATTGATATCTTCGAGGCTTTTGACGAGCTTGTGTTGATGAAGCGAGGAGGACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGCCATTCCAGGCGTTCCCAAAAT
CACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGCGTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAAATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAACCGAATTTTCACAATCCTTTG
CAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTACAATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATCTTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGTTGTTTGCGGCGGTGCTATTCATT
GGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAGAGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTACTGATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGATCGACTTCGAGTGGAAGGCAAC
CAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATTGTGTCCTCGGCTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGAAAATTCCGGTATGGTGGAGATGGTATTACTGGG
CATGCCCTGTTGCATGGACTCTGTACGGCTTGATAGCATCGCAGTTCGGGG


>TRINITY_DN121424_c0_g1_i8 from sample A3 / Example unspliced isoform:
CGCCAACATGATCGAGgtagtgtttgcatacgaaatgaactatctttatacattttaataggctcttttctttttctttttctaaacgtatattctttatcttgattttcagGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCA
GGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAA
ACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGC
CGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGG
TTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAGTAC
TGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGA
AGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGA
CAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACA
GTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGC
AGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGGCAT
TAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCT



Intron 4 GTACAGCACA ... TCTTGTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A115.2136413.970
A212.9131217.348.2
A312.448216.371.8
A414.0322913.339.3
A514.7323414.441.4
A613.521172.819.6
A713.313115.251.1
A814.18630.811.2
A914.642715.250
A108.194421140.8



>TRINITY_DN122294_c1_g3_i1 from sample A2 / Example spliced isoform
CGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTTA
ATCTTCAACTCCCAACCAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTTATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCCTACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAAC
AACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGT
TCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTCTCCAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCT
TGCAAATGTACATGACAAAGGGGATATCGATCACAATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATT
TGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGTCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGC
AAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGG
GTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCA
TTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGAT
CTCTTCGACGATGTCATTCTACTTTCGGAAGGTCACATTATTTATCAGGGTCAGCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTTGCAGATTTCTTGCAGGAGGTGCGTTAATTTTTGGTCTTCCTTCAAAAATTTCTTTGTCGGCTGCGACATTAAGATGAGTGTGT
CTGATTAATATCATTTTATTGTGTTGAATCGATTGAACAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATC
CCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGCGACGGAGGC
ATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTAT
CACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAG
ATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTC
AATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATC


>TRINITY_DN122294_c1_g3_i3 from sample A2 / Example unspliced isoform:
CGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTTA
ATCTTCAACTCCCAACCAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTTATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCCTACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAAC
AACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGT
TCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTCTCCAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCT
TGCAAATGTACATGACAAAGGGGATATCGATCACAATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATT
TGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGTCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGC
AAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGG
GTCGAGATATGGTATGGATGCTTTTTCTCTTTCTGGATCATATTGAATCCGAAGCTACTTTAGTTTTTTAAGTCAGTTGGATTAACCGGCTATCTTCTTTTCTGTAGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGgtacagcacaggcagc
tgttttgaaatcgattaggattaaatgaatttcaatgaatttactgagaatggttgaatattttcttgtgcagGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGGTATTTTTTTATACCTGCTTTATCTCTAATGGTGTTGTTCCTGTTAACTAAAAATCCTCCTTTCATTAGAATC
TGGGTGAATAAAGATTTGTGATTAGTGTTTTGCTCAGATTGCAATTTTAACATTTATTAGCATAGCAGTTTAAAATTGTTAAGATTGCAGTATCAAAATGTTAGAAACTGTAAAATCTAAATTCAGGCCCAAAAAAGTGTGAAAAAAGAGGCCTTTTAGTTGAACTTTAGCCCACGACTCAAAAGAAACGTCTCGAATTC
AATTTTCTTTGGATGAACCATCAATCTTTGTGTTGCTGTTGATGGTTGGATCAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGG
TCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATTCTACTTTCGGAAGGTCACATTATTTATCAGGGTC



Intron 5 GTATTTTTTT ... GTTGGATCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A110.47342440
A212.7275440
A310.68460255
A49.69205222
A59.87201922
A63.8110104
A77.78308026
A810.676708
A97.69252121
A107.64411134



>TRINITY_DN122294_c1_g3_i1 from sample A2 / Example spliced isoform
CGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTTA
ATCTTCAACTCCCAACCAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTTATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCCTACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAAC
AACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGT
TCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTCTCCAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCT
TGCAAATGTACATGACAAAGGGGATATCGATCACAATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATT
TGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGTCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGC
AAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGG
GTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCA
TTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGAT
CTCTTCGACGATGTCATTCTACTTTCGGAAGGTCACATTATTTATCAGGGTCAGCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTTGCAGATTTCTTGCAGGAGGTGCGTTAATTTTTGGTCTTCCTTCAAAAATTTCTTTGTCGGCTGCGACATTAAGATGAGTGTGT
CTGATTAATATCATTTTATTGTGTTGAATCGATTGAACAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATC
CCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGCGACGGAGGC
ATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTAT
CACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAG
ATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTC
AATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATC


>TRINITY_DN122294_c1_g3_i3 from sample A2 / Example unspliced isoform:
CGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTTA
ATCTTCAACTCCCAACCAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTTATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCCTACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAAC
AACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGT
TCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTCTCCAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCT
TGCAAATGTACATGACAAAGGGGATATCGATCACAATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATT
TGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGTCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGC
AAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGG
GTCGAGATATGGTATGGATGCTTTTTCTCTTTCTGGATCATATTGAATCCGAAGCTACTTTAGTTTTTTAAGTCAGTTGGATTAACCGGCTATCTTCTTTTCTGTAGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGTACAGCACAGGCAGC
TGTTTTGAAATCGATTAGGATTAAATGAATTTCAATGAATTTACTGAGAATGGTTGAATATTTTCTTGTGCAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGgtatttttttatacctgctttatctctaatggtgttgttcctgttaactaaaaatcctcctttcattagaatc
tgggtgaataaagatttgtgattagtgttttgctcagattgcaattttaacatttattagcatagcagtttaaaattgttaagattgcagtatcaaaatgttagaaactgtaaaatctaaattcaggcccaaaaaagtgtgaaaaaagaggccttttagttgaactttagcccacgactcaaaagaaacgtctcgaattc
aattttctttggatgaaccatcaatctttgtgttgctgttgatggttggatcagATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGG
TCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATTCTACTTTCGGAAGGTCACATTATTTATCAGGGTC



Intron 6 GTAAACCGAA ... AATTTGACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A120.64135.9435
A225.42121.3341
A326.17181.6764
A416.85134.3127
A518.1455.1012
A620.7426.907
A727.08110.9141
A821.771504
A928.9968.7028
A1022.9697.7229



>TRINITY_DN122294_c1_g3_i1 from sample A2 / Example spliced isoform
CGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTTA
ATCTTCAACTCCCAACCAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTTATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCCTACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAAC
AACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGT
TCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTCTCCAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCT
TGCAAATGTACATGACAAAGGGGATATCGATCACAATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATT
TGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGTCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGC
AAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGG
GTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCA
TTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGAT
CTCTTCGACGATGTCATTCTACTTTCGGAAGGTCACATTATTTATCAGGGTCAGCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTTGCAGATTTCTTGCAGGAGGTGCGTTAATTTTTGGTCTTCCTTCAAAAATTTCTTTGTCGGCTGCGACATTAAGATGAGTGTGT
CTGATTAATATCATTTTATTGTGTTGAATCGATTGAACAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATC
CCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGCGACGGAGGC
ATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTAT
CACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAG
ATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTC
AATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATC


>TRINITY_DN122294_c1_g3_i4 from sample A2 / Example unspliced isoform:
CCAACATGATCGAGGTAGTGTTTGCATACGAAATGAACTATCTTTATACATTTTAATAGGCTCTTTTCTTTTTCTTTTTCTAAACGTATATTCTTCATCTTGATTTTCAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGg
taaaccgaatttcattcttaaatttctgccaatttcataccgaaatcgcatgattctttaccagtaaataaatatgactgaaatttaatcaaaaaatcctgcttgcaagtctttaaccctttgaagctttcattaaatttgacagGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCATGGCT
CTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGGTATGGATGC
TTTTTCTCTTTCTGGATCATATTGAATCCGAAGCTACTTTAGTTTTTTAAGTCAGTTGGATTAACCGGCTATCTTCTTTTCTGTAGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGG



Intron 7 GTGCGTTAAT ... GATTGAACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A121.75230.450.178
A226.4214331.771
A322.4323635.4101
A422.6618929.881
A522.5510413.647
A622.34799.436
A724.1716722.484
A822.49494.424
A924.6719026.498
A1022.27199.924.690



>TRINITY_DN122294_c1_g3_i9 from sample A2 / Example spliced isoform
CGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTTA
ATCTTCAACTCCCAACCAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTTATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCCTACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAAC
AACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGT
TCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTTTCTAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCT
TGCAAATGTACATGACAAAGGGGTTATCGATCATGATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATT
TGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGCCAACATGATCGAGGTAGTGTTTGCATACGAAATGAACTATCTTTATACATTTTAATAGGCTCTTTTCTTTTTCTTTTTCTAAACGTATATTCTTCATCTTGATTTTCAGGCAGTTCTTGGTTCGCTTCATTTGTAC
AAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACA
GAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGG
GCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACA
ACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATTCTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGA
GAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTC
TGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTT
GGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTA
TACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTA
AAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTA
TTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGGCATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGTTAGGGCAAGTTC
ACGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCTGGAAAGAAAGGAATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACATGCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGA
CATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCAAGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTT
ACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATACTGAGTTAAGAAAGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTCGGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGT
TGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCTGCTGCTATTGTGATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACCCAGCATTGATATCTTCGAGGCTTTTGACGAGCTTGTGTTGATGAAGCGAGGAGGACAGATGATATACG
TTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGCCATTCCAGGCGTTCCCAAAATCACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGCGTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAAATGAAGCTCTGATC
AAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAACCGAATTTTCACAATCCTTTGCAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTACAATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATCTTTTGGAGAATGGG
TCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGTTGTTTGCGGCGGTGCTATTCATTGGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAGAGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTACTGATCGAAGTGCCAT
ACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGATCGACTTCGAGTGGAAGGCAACCAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATTGTGTCCTCGGCTTTCTATTCCATC
TGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGAAAATTCCGGTATGGTGGAGATGGTATTACTGGGCATGCCCTGTTGCATGGACTCTGTACGGTTTGATTGCATCACAGTTCGGGGATTATGTTACGCCCATGAATAGAACAGACGGAACTACACAGCCATTGAATGAGTTTCTCAGAAGTTATTTTGGATTCAA
ACATAGCTTTTTGGGACCTGTTGCAGTTGTGACAGCAGGATGGAGCGTTCTCTTCGCATTCATCTTCGCCTTCTCCATCAAGAAGCTCAACTTCCAGTA


>TRINITY_DN122294_c1_g3_i1 from sample A2 / Example unspliced isoform:
CGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTTA
ATCTTCAACTCCCAACCAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTTATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCCTACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAAC
AACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGT
TCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTCTCCAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCT
TGCAAATGTACATGACAAAGGGGATATCGATCACAATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATT
TGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGTCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGC
AAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGG
GTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCA
TTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGAT
CTCTTCGACGATGTCATTCTACTTTCGGAAGGTCACATTATTTATCAGGGTCAGCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTTGCAGATTTCTTGCAGGAGgtgcgttaatttttggtcttccttcaaaaatttctttgtcggctgcgacattaagatgagtgtgt
ctgattaatatcattttattgtgttgaatcgattgaacagGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATC
CCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGCGACGGAGGC
ATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTAT
CACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAG
ATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTC
AATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATC



Intron 8 GTGAGCTTTC ... ATGATTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A122.36121.5356
A226.0486.230.30
A323.9795.4300
A418.2560.913.60
A534.8726.2141.3
A622.52573.4166.32
A723.09552.8165.66
A820.26741.5187.914.2
A920.7562.9146.66.5
A1021.33374.7101.32.5



>TRINITY_DN45700_c1_g1_i6 from sample A8 / Example spliced isoform
GAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTTAATCTTCAACTCCCAACCAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTTATGGGGCGTTCCTTGTGCGACAAGTAAGAGA
GATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCCTACCGTTTCTCTATATAAATTTGAGTGTTACTCGTAACAACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAA
GATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGTTCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGC
GTTTTCTCCAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCTTGCAAATGTACATGACAAAGGGGATATCGATCACAATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAG
GCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATTTGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGTCAACATGATCGAGGCAGTTCTTGGTT
CGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGAT
GAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGC
CACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTC
TGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGA
TTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATC
CCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACG
GAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTATTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGTTGGAGTCTGTGCTGTGGATC
ATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATC
CAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGG
GATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGGCATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCT
GTTAGGGCAAGTTCACGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCTGGAAAGAAAGGAATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACATGCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCG
TGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCAAGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCC
ATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATACTGAGTTAAGAAAGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTCGGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATA
GCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCTGCTGCTATTGTGATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACCCAGCATTGATATCTTCGAGGCTTTTGACGAGCTTGTGTTGATGAAGCGAGGAGG
ACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGCCATTCCAGGCGTTCCCAAAATCACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGCGTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAA
ATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAACCGAATTTTCACAATCCTTTGCAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTACAATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATC
TTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGATGTTTGCGGCGGTGCTATTCATTGGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAGAGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTACT
GATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGATCGACTTCGAGTGGAAGGCAACCAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATTGTGTCCTCGG
CTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGAAAATTCCGGTATGGTGGAGATGGTATTACTGGGCATGCCCTGTTGCATGGACTCTGTACGGCTTGATAGCATCGCAGTTCGGGGAT


>TRINITY_DN45700_c1_g1_i4 from sample A8 / Example unspliced isoform:
GAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTTAATCTTCAACTCCCAACCAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTTATGGGGCGTTCCTTGTGCGACAAGTAAGAGA
GATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCCTACCGTTTCTCTATATAAATTTGAGTGTTACTCGTAACAACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAA
GATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGTTCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGC
GTTTTTTCTAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCTTGCAAATGTACATGACAAAGGGGTTATCGATCATGATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAG
GCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATTTGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGTCAACATGATCGAGGCAGTTCTTGGTT
CGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGAT
GAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGC
CACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTC
TGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGA
TTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATC
CCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACG
GAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTATTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGTTGGAGTCTGTGCTGTGGATC
ATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATC
CAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGG
GATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGGCATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCT
GTTAGGGCAAGTTCACGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCTGGAAAGAAAGGAATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACATGCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCG
TGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCAAGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCC
ATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATACTGAGTTAAGAAAGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTCGGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATA
GCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCTGCTGCTATTGTGATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACCCAGCATTGATATCTTCGAGGCTTTTGACGAGCTTGTGTTGATGAAGCGAGGAGG
ACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGCCATTCCAGGCGTTCCCAAAATCACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGCGTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAA
ATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAACCGAATTTTCACAATCCTTTGCAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTACAATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATC
TTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGATGTTTGCGGCGGTGCTATTCATTGGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAGAGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTACT
GATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGATCGACTTCGAGTGGAAGGCAACCAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATTGTGTCCTCGG
CTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGgtgagctttcatgattttaataggtcatatattcctattctagaaatctgatctgacataatatgttcactcaaacccataaaaaaacatcaaaattccatcattatgacaaatttattaccagccaactgtaccaagtccattgatgta
acttgtttgcttagaacttaagaaaagatcaaaattccatcactatcgccatgtattgcaaagttgtacatccaattaagatttgcccatacaactgtgtgtatatctgaaaatatacaaccataagaaaacatagcagggcaaatattgcttacgcattcaattacttttcctttgtcaggattcaattcaattaagat
ctctactaattaattaatttgaatgattgcagAAAATTCCGGTATGGTGGAGATGGTATTACTGGGCATGCCCTGTTGCATGGACTCTGTACGGCTTGATAGCATCGCAGTTCGGGGAT



Intron 9 GTATGTATTA ... AAATTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A119.82413386
A216.511772545
A318.642034251
A418.921954546
A5201323630
A617.2451698117
A716.86688124155
A817.0956583150
A917.12627104155
A101755913099



>TRINITY_DN121424_c0_g1_i4 from sample A3 / Example spliced isoform
CTGTCCTCTCGTCCTATGTGTAGGTAGTTACGGAGTACACGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAA
TGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTAATCTTAAACTCCCAACCAGCAATGAAGAACTGAGAGGCCCAACGGCGATAAAAAAATTGATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCC
TACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAACAACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTG
ATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGTTCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTTTCTAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAA
AAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCTTGCAAATGTACATGACAAAGGGGTTATCGATCATGATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGG
CATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATTTGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGCCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCG
GCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGA
GAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATG
CGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGG
TGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAG
TACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAG
AGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGA
TGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTC
ACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAAT
TGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGG
CATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGTCAGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCTGGAAAGAAAGGA
ATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACATGCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGT
TCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCAAGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATACTGAGTTAAGAA
AGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTCGGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCTGCTGCTATTGTG
ATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACCCAGCATTGATATCTTCGAGGCTTTTGACGAGCTTGTGTTGATGAAGCGAGGAGGACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGCCATTCCAGGCGTTCCCAAAAT
CACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGCGTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAAATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAACCGAATTTTCACAATCCTTTG
CAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTACAATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATCTTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGTTGTTTGCGGCGGTGCTATTCATT
GGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAGAGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTACTGATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGATCGACTTCGAGTGGAAGGCAAC
CAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATTGTGTCCTCGGCTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGAAAATTCCGGTATGGTGGAGATGGTATTACTGGG
CATGCCCTGTTGCATGGACTCTGTACGGCTTGATAGCATCGCAGTTCGGGG


>TRINITY_DN121424_c0_g1_i2 from sample A3 / Example unspliced isoform:
CTGTCCTCTCGTCCTATGTGTAGGTAGTTACGGAGTACACGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAA
TGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTAATCTTAAACTCCCAACCAGCAATGAAGAACTGAGAGGCCCAACGGCGATAAAAAAATTGATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCC
TACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAACAACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTG
ATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGTTCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTTTCTAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAA
AAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCTTGCAAATGTACATGACAAAGGGGTTATCGATCATGATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGG
CATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATTTGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGCCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCG
GCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGA
GAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATG
CGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGG
TGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAG
TACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAG
AGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGA
TGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTC
ACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAAT
TGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGG
CATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGTTAGGGCAAGTTCACGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCT
GGAAAGAAAGGAATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACATGCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAAAAACAAC
CCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCAAGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATA
CTGAGTTAAGAAAGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTCGGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCT
GCTGCTATTGTGATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACCCAGCATTGATATCTTCGAGGCTTTTGACGAGgtatgtattaagaatagactcgtagcaccaaaaaataaaacaatgtcatttattttaaaggtatttcagctacattttggtgtttttttcttcaa
atttaacaaactatataaactaaaacctttatagctaaatgaaatcattcagtatgcctatgaaactcaattgtttaagtggtaataacaagaatacaattgcaaaatttgcagCTTGTGTTGATGAAGCGAGGAGGACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGC
CATTCCAGGCGTTCCCAAAATCACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGCGTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAAATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAA
CCGAATTTTCACAATCCTTTGCAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTACAATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATCTTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGTTG
TTTGCGGCGGTGCTATTCATTGGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAGAGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTACTGATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGAT
CGACTTCGAGTGGAAGGCAACCAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATTGTGTCCTCGGCTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGAAAATTCCGGTAT
GGTGGAGATGGTATTACTGGGCATGCCCTGTTGCATGGACTCTGTACGGCTTGATAGCATCGCAGTTCGGGG



Intron 10 GTATGGATGC ... TTTTCTGTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A118.1929171.324.7
A215.8928057.130.2
A316.5535676.944.2
A415.8522044.816.2
A517.6817841.527.9
A618.157819.15.9
A716.5822849.427.4
A818.68256.42.4
A917.0517940.217.1
A101428750.141.8



>TRINITY_DN133194_c0_g2_i3 from sample A1 / Example spliced isoform
ACGGAGTACACGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTAC
TTTTATTTTAATCTTCAACTCCCAACCAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTTATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCCTACCGTTTCTCTATATAAATTTGAGTGT
TACTCGTAACAACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTA
AAATATGAGTTCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTCTCCAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAA
CTGCGATTCTTGCAAATGTACATGACAAAGGGGATATCGATCACAATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGC
TACGAACATTTGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGTCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCC
AGCCTCGGGCAAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCC
AGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATG
CATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGA
AACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTTGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACC
GTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCG
TTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGCATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTATTA
CAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTT
TTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGA
TGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGGCATTAATTTCTGAAGATGATTTGAAACA
AAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGTTAGGGCAAGTTCACGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCTGGAAAGAAAGGAATGGTTTTACCTTTTC
AACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACATGCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGTTCTTGCAGGAAGGAAA
ACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCAAGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATACTGAGTTAAGAAAGACGTTTGTGGAGGA
AGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTCGGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCTGCTGCTATTGTGATGAGGACTGTCCGAA
ACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACCCAGCATTGATATCTTCGAGGCTTTTGACGAGCTTGTGTTGATGAAGCGAGGAGGACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGCCATTCCAGGCGTTCCCAAAATCACCCATGGATACAAC
CCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGCGTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAAATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAACCGAATTTTCACAATCCTTTGCAATTCAGTCCTTGGC
CTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTACAATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATCTTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGTTGTTTGCGGCGGTGCTATTCATTGGTGTGAACAATGCTT
CTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAGAGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTACTGATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGATCGACTTCGAGTGGAAGGCAACCAAGTTCCTGTGGTTT
TTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATTGTGTCCTCGGCTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGAAAATTCCGGTATGGTGGAGATGGTATTACTGGGCATGCCCTGTTGCATG
GACTCTGTACGGTTTGATTGCATCACAGTTCGGGGATTATGTTACGCCCATGAATAGAACAGACGGAACTACACAGCCATTGAATGAGTTTCTCAGAAGTTATTTTGGATTCAAACATAGCTTTTTGGGACCTGTTGCAGTTATGACAGCAGGATGGAGCGTTCTCTTCGCATTCATCTTCGCCTTCTCCATCAAGAAGC
TCAACTTCCAGTCAAGATAATTACTGGAATATCAGTTATTATTTTTCCAATGTGTCATTACCAGTGACA


>TRINITY_DN133194_c0_g2_i10 from sample A1 / Example unspliced isoform:
CCAGGGCGTCGGGTCGAGATATGgtatggatgctttttctctttctggatcatattgaatccgaagctactttagttttttaagtcagttggattaaccggctatcttcttttctgtagACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCA
CTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTG
GACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATT
TAAATGCCCTGAGAGGAAAGGTGTTGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCC
ATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGA
GCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGCATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTATTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCAT
TATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCA
GAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTG



Intron 11 GTATGGTCTG ... TCCCCTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A117.692285345
A217.241082916
A318.031914836
A421.531644248
A522.89962532
A615.9821516
A715.61923338
A816.6760159
A9201704540
A1018.422175840



>TRINITY_DN109713_c0_g1_i10 from sample A4 / Example spliced isoform
AGCGTGTCCACATGAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTTAATCTTCAACTCCCAACCAGCAATGAAGCACTGACAGGCCCAACGGCGATAAAAAAATTGATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCA
AATGAACACATGCGATCTTTTTCTTCCTACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAACAACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCA
ACAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGTTCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTTTCTAAATCATCCAGTACTCGACAACGCCAGAATGATGAG
GAAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCTTGCAAATGTACATGACAAAGGGGTTATCGATCATGATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAA
GCTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATTTGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGCCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCC
ATGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGAC
TTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGT
TTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAG
TTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAG
GTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAAT
GGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGA
TGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGA
TTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTC
TCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATT
ATCTAGATCCTCTAGGAAAGCCCCAGGCATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGTTAGGGCAAGTTCACGGGCAAGTTCACAACGAGGACGAAATAGC
AATATTTCAGATTCTATTCGTGCATCTGGAAAGAAAGGAATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACATGCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAAT
GGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCAAGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCT
GGCTTCGTCTTCCGGCCGAAGTCGATACTGAGTTAAGAAAGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTCGGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAG
CCAACATCAGGACTGGATGCGAGAGCTGCTGCTATTGTGATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACCCAGCATTGATATCTTCGAGGCTTTTGACGAGCTTGTGTTGATGAAGCGAGGAGGACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACT
GATCGACTACTTTGAGGCCATTCCAGGCGTTCCCAAAATCACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGCGTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAAATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTA
AAGATATCTACTTCGAAACCGAATTTTCACAATCCTTTGCAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTACAATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATCTTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTG
AATGCTATGGGGTCGTTGTTTGCGGCGGTGCTATTCATTGGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAGAGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTACTGATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGT
AATCGTGTATGCAATGATCGACTTCGAGTGGAAGGCAACCAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATTGTGTCCTCGGCTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTC
GACCGAAAATTCCGGTATGGTGGAGATGGTATTACTGGGCATGCCCTGTTGCATGGACTCTGTACGGTTTGATTGCATCACAGTTCGGG


>TRINITY_DN109713_c0_g1_i3 from sample A4 / Example unspliced isoform:
AGCGTGTCCACATGAACAACCGACAATGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTAATCTTAAACTCCCAACCAGCAATGAAGAACTGAGAGGCCCAACGGCGATAAAAAAATTGATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAA
ATGAACACATGCGATCTTTTTCTTCCTACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAACAACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAA
CAACAAATTGTTGGACCTTATTATTGATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGTTCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTTTCTAAATCATCCAGTACTCGACAACGCCAGAATGATGAGG
AAGCGCTCAAATGGGCGGCCATTGAAAAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCTTGCAAATGTACATGACAAAGGGGTTATCGATCATGATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAG
CTTCGAAAGAGGATTGACAGTGTTGGCATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATTTGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGCCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCA
TGATGTCAGTGGCATCATCAAGCCCGGCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACT
TGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTT
TTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGT
TGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGG
TGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATG
GAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGAT
GTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGgt
atggtctgatggcccttgaagacccactttcctatgctctgattattgtgattcttaatgctcaaatctaatgtcaagtttcctgggtattctcttcccctgcagATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGG
CTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTG
GAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGGCATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAA
TACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGT



Intron 12 GTGAGTAAAT ... ATTGTTACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A117.6192041
A216.67140028
A318.72165138
A414.29180030
A520.99128034
A620128532
A720.19249063
A811.5492012
A919.45294071
A1017.85290063



>TRINITY_DN45077_c1_g1_i2 from sample A6 / Example spliced isoform
GAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGAGAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAG
AGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATGCGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGG
AAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGGTGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCAC
ATTATTTATCAGGGTCAGCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAGTACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCA
CATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAGAGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTA
TTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGATGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTT
CCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTCACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACAC
TTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAATTGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTC
GCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGGCATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAAT
GAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGTTAGGGCAAGTTCACGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCTGGAAAGAAAGGAATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACAT
GCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAAAAACAACCCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCA
AGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATACTGAGTTAAGAAAGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTC
GGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCTGCTGCTATTGTGATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACC
CAGCATTGATATCTTCGAGGCTTTTGATGAGCTTGTGTTGATGAAGCGAGGAGGACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGCCATTCCAGGCGTTCCCAAAATCACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGC
GTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAAATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAACCGAATTTTCACAATCCTTTGCAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTAC
AATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATCTTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGATGTTTGCGGCGGTGCTATTCATTGGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAG
AGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTAAACCCCTAATATCATCCCCCCATTAACGCCTATAACTTTTTTACTTCTTCTGGTTTTTAATTTAACACCACCCATCAATGCCAATAAGCTTTTTCAAACCTTTTTTCATCACTAATTGGAATGTGGTCATGGTTCAATAATGATGTTA
TGCAGGTACTGATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGATCGACTTCGAGTGGAAGGCAACCAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATT
GTGTCCTCGGCTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGAAAATTCCGGTATGGTGGAGATGGTATTACTGGGCATGCCCTGTTGCATGGACTCTGTACGGTTTGATTGCATCACAGTTCGGGGATTATGTTACGCCCATGAATAGAACAGACGGAACTACACAGCCATTGAATGAGTT
TCTCAGAAGTTATTTTGGATTCAAACATAGCTTTTTGGGACCTGTTGCAGTTGTGACAGCAGGATGGAGCGTTCTCTTCGCATTCATCTTCGCCTTCTCCATCAAGAAGCTCAACTTCCAGTCAAGATAATTACTGG


>TRINITY_DN45077_c1_g1_i4 from sample A6 / Example unspliced isoform:
TCTTGGCAAGCAGATGGCAAAAGgtgagtaaattacagactagatgaatttgcaaatgaaaatctctcctgtgatagaaggaaataatgattttgtttcttaaattgttacagCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATG
GTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGGCATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGA
TTCTGT



Intron 13 GTATGAATGA ... TTTTTTGAAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A19.27225023
A210.33165119
A312.5189027
A413.85168027
A512.17101214
A611.36156020
A712.35298042
A89.68112012
A97.91233020
A109.15278028



>TRINITY_DN121424_c0_g1_i1 from sample A3 / Example spliced isoform
CTGTCCTCTCGTCCTATGTGTAGGTAGTTACGGAGTACACGATCAAAGAGGGACGGCGATCATGATTCACAAGCATCCGCTGAAAAGGCAGATCTAAATCCCAAATAGTCCGATTTGACCTAAAATATTGAAATATTCACAATCCTGAAAATTGCCTCCTTCTTGATCGAATAGCGCGTGTCCACATCAACAACCGACAA
TGTTGAATGTGACCCATCTAGAAGCCTACTTTTATTTAATCTTAAACTCCCAACCAGCAATGAAGAACTGAGAGGCCCAACGGCGATAAAAAAATTGATGGGGCGTTCCTTGTGCGACAAGTAAGAGAGATGATCTGAGCATTGCCATGGAATGTTCGCTTGTTGGGAAGTCAAATGAACACATGCGATCTTTTTCTTCC
TACCGTTTCGCTATATAAATTTGAGTGTTACTCGTAACAACCCATTAAGCTTCTGATAGCATCAGCACGGGTCATTAAGCTTCTGATAGCATCAGCACGGGTTGGGTTCTCAAGCTATTTTTACAGAAGATCTCACAGGGGATTCATTCACAGGGCCTGAAAATCTAAGTGCAACAACAAATTGTTGGACCTTATTATTG
ATTGAATCTGGATTCTCTGGAAAGGCTAAAATATGAGTTCGTCCATCACCGAGCATGAGCCTGATGATAAAACATGGACAGGCAGTCTGCGTTCAGCGAGTACCAGATTGTTCGAACGATCCGACAGCGTTTTTTCTAAATCATCCAGTACTCGACAACGCCAGAATGATGAGGAAGCGCTCAAATGGGCGGCCATTGAA
AAGCTCCCCACTTACGACCGGTTAAGAACTGCGATTCTTGCAAATGTACATGACAAAGGGGTTATCGATCATGATCAGATTGACGTTCGGGGTATCGAACTCGAGACCCGCCAACAGTTGATGGAGAGGCTCGTCAAAGTTGCAGACGAGGATAATGAACGCTTTCTTAACAAGCTTCGAAAGAGGATTGACAGTGTTGG
CATCGTTTTGCCAGAGATCGAAGTTCGCTACGAACATTTGAACATCGATGCGAATGCCTACGTAGGTGGCAGAGCGCTGCCCACTCTTACCAACTACACCGCCAACATGATCGAGGCAGTTCTTGGTTCGCTTCATTTGTACAAGAGCAACAAGGCAACCATGACAATTCTCCATGATGTCAGTGGCATCATCAAGCCCG
GCAGGATGACCCTTCTCCTTGGACCGCCAGCCTCGGGCAAGACTACTTTGCTCATGGCTCTTGCGGGAAAACTGGAGAAAAGTCTCAAAGTTACAGGTGGAGTAAAATACAATGGACATACCATGGATGAATTCGTGCCACAGAGAACATCCGCTTACATAAGTCAAAATGACTTGCACATTGGTGAAATGACCGTGAGA
GAAACCTTAGATTTCTCTGCACGGTGCCAGGGCGTCGGGTCGAGATATGACGTATTGACTGAACTTGTGAGGAGAGAAAAGGATGGAGGAATCAAACCAGATCCCGATATAGATGTCTTTATGAAGGCCACTGCATTAGAGGGCCAGAAGGCCAACATGATTACGGACTATGTTTTGAAGATCTTGGGGCTGGACATATG
CGCCGACACACTAGTAGGAGATGCAATGCATAGAGGCATTTCTGGAGGGCAGAAGAAAAGAGTTACAACTGGGGAAATGATTGTTGGAGCTTCGAAAGCCCTGTTCATGGACGAGATTTCCACTGGTCTGGACAGCGCTACAACATACCAGATTGTTCGATGCCTACGCCAAGTTGTCCATGTTTTCATGTCGACGATGG
TGGTTTCACTCCTGCAACCTGCCCCTGAAACCTTCGATCTCTTCGACGATGTCATACTACTTTCGGAAGGTCACATTATTTATCAGGGTCCTCGAGAAAACATTCTCGAGTTCTTCGAGTCCATGGGATTTAAATGCCCTGAGAGGAAAGGTGTCGCAGATTTCTTGCAGGAGGTGACTTCTCTCAAAGATCAAGAGCAG
TACTGGGCAAACAGAAGGCAAACGTACCGTTATGTTCCAGTGAAGGAGTTTGCAGATGCATTCCAGTCATTTCACATTGGTACCGCATTGAATTCAGAGTTATCTATTCCATACGATAAGAAGAAATCCCATCCCGCGGCTCTGACAACAGAGGAATACGGTTTGAGCAAAATGGAACTTTTCAGAGCTTGCTTTGATAG
AGAAGTTTTGCTGATGAAGAGAAGTTCGTTCGTCTACATCTTCAAAACTGTTCAGATATTTGTCATTGCTTCTATTGCCATGAGCGTCTTTTTCCGTACCAATATGCATCACAGAAATCTGGGTGACGGAGCCATTTACTTTGGAGCGCTTTTCTTTGGTCTAGTGGTGGTGATGTTCAATGGAATGGCAGAATTGTCGA
TGACAATTGACAAGCTTCCAGTGTTTTACAAGCAAAGAGATTTCAAGTTCTACCCAGCCTGGGCTTATTCGCTTCCGACATGGATTATGAGAATTCCTCTTTCCCTGCTGGAGTCTGTGCTGTGGATCATTATCACATATTATACCATAGGTTTTGCCCCAAATCCTCAGAGATTTTTCCGACAACTTGTTCTGTTCTTC
ACAGTCCATCAGATGTCTTTAGGTCTTTTTCGCTTTATAGCTGCTGTGGGACGCAATCGGATAGTGGCTAACACTTTCGGTTCGTTTGCCCTCTTGATCATATTCGTTCTCGGAGGATTCATCATATCCAGAGATGATATTAAAGGATGGTGGATATGGGGCTACTGGATTTCTCCTCTCATGTATGCTCAAAATGGAAT
TGCAGTAAACGAGTTCTTGGCAAGCAGATGGCAAAAGCCAGGTGATAACGTTGGCGTCAATTTATTGGAATCTCGCGGGCTTTTTGCGGAGAGCTACTGGTATTGGCTTTCTATTGGAGCCATGGTGGGATTCAATATTTTATTTAACTTCCTCTACACGTTGGCATTGCATTATCTAGATCCTCTAGGAAAGCCCCAGG
CATTAATTTCTGAAGATGATTTGAAACAAAAGCAAGCAGCTCTAACCGGCGTTGTCCAAAGAAACAATACAAATGAAATGCAGACGATGTCAGGAGAAAGCCATGAAGAAATTCAAGCTTCTGATTCTGTTAGGGCAAGTTCACGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCGTGCATCT
GGAAAGAAAGGAATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACATGCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAAAAACAAC
CCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCAAGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAAGTCGATA
CTGAGTTAAGAAAGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTCGGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGCGAGAGCT
GCTGCTATTGTGATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACCCAGCATTGATATCTTCGAGGCTTTTGACGAGCTTGTGTTGATGAAGCGAGGAGGACAGATGATATACGTTGGTCCTCTTGGCCGCCATTCCCAATCACTGATCGACTACTTTGAGGCCATTCCAGG
CGTTCCCAAAATCACCCATGGATACAACCCTGCAACTTGGATGCTTGAAGCGTCTTCTGTTGGCGCAGAATTGCGTCTTGGAGTCGATTTTGCAGAAGTATACAGGAATTCATCCCTCTACCAGCGAAATGAAGCTCTGATCAAGGAACTCGGCGTACCGGCTCCAGGAACTAAAGATATCTACTTCGAAACCGAATTTT
CACAATCCTTTGCAATTCAGTCCTTGGCCTGTTTGTGGAAGCAGCACTGGTCATACTGGAGAAATCCCGAGTACAATGCTGTCCGTTTCTTTTTCACTCTGGTCACTGCTGTGCTTTTCGGCACCATCTTTTGGAGAATGGGTCAAAAGATAACGGAGCAACAAAAGGTTTTGAATGCTATGGGGTCGTTGTTTGCGGCG
GTGCTATTCATTGGTGTGAACAATGCTTCTTCAGTCCAGCCTGTGGTGGATGTTGAAAGGACAGTTTTCTACAGAGAAAAGGCAGCAGGGATGTACTCGGCCCTTCCTTATGCCCTAGCTCAGGTACTGATCGAAGTGCCATACGTCTTTGTTCAAGCGGCGGTGTATGGAGTAATCGTGTATGCAATGATCGACTTCGA
GTGGAAGGCAACCAAGTTCCTGTGGTTTTTCTTCTTCATGTTCTTTACATTTTTGTACTACACATACTATGGAATGATGACGGTAGCTCTGACTCCCAATGTGAACATTGCTGCCATTGTGTCCTCGGCTTTCTATTCCATCTGGATGCTTTTCTGCGGTTTCATAATTCCTCGACCGAAAATTCCGGTATGGTGGAGAT
GGTATTACTGGGCATGCCCTGTTGCATGGACTCTGTACGGCTTGATAGCATCGCAGTTCGGGG


>TRINITY_DN121424_c0_g1_i7 from sample A3 / Example unspliced isoform:
CCATGAAGAAATTCAAGCTTCTGgtatgaatgatatgaacaaattctgcaaagaatattaactttcaatttcatacggtgaaatacttgccataaaattctttaaaactaatgtagcaatttttttgaagATTCTGTTAGGGCAAGTTCACGGGCAAGTTCACAACGAGGACGAAATAGCAATATTTCAGATTCTATTCG
TGCATCTGGAAAGAAAGGAATGGTTTTACCTTTTCAACCTTTGGCGATGGCCTTTGACAATGTCAATTATTTTGTAGACATGCCCCCGGAAATGAAGCAGCAAGGAGTTGAAGAAAGTAGACTCCAGTTATTGCGTGGAACGAGTGGGACATTCAGGCCTGGCGTGCTCACCTGTCTAATGGGTGTGAGTGGGGCTGGAA
AAACAACCCTAATGGATGTTCTTGCAGGAAGGAAAACTGGTGGATACATAGAAGGGTCCATCACCATCTCCGGTTATCCCAAGAAACAAGAAACCTTTGCTCGCATCTCTGGTTACTGCGAACAAACTGATATCCATTCTCCTTTTGTTACTGTTTATGAGTCTCTCGTATACTCTGCCTGGCTTCGTCTTCCGGCCGAA
GTCGATACTGAGTTAAGAAAGACGTTTGTGGAGGAAGTTATGGATCTTGTGGAGCTGAATAACCTGAGACAAGCTCTGGTCGGTCTTCCTGGAGTGAATGGCCTGTCCACGGAACAACGTAAGAGATTGACAATAGCAGTTGAATTGGTTGCAAATCCTTCAATAATCTTCATGGACGAGCCAACATCAGGACTGGATGC
GAGAGCTGCTGCTATTGTGATGAGGACTGTCCGAAACACTGTGGATACAGGCCGAACTGTTGTGTGCACTATTCATCAACCCAGCATTGATATCTTCGAGGCTTTTGACGAG