NA ----> NA

Intron 1 GTATGTATGC ... TACTATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	9.5	2
A2	71.43	3	6	9
A3	65.59	5.5	9.8	11
A4	89.72	1	8.5	9
A5	71.43	1	2	3
A6	49.84	83	48	117
A7	53.66	68	50.8	106.8
A8	51.14	94	63	133.8
A9	51.94	96	65.5	142
A10	61.99	39.3	52	76

>TRINITY_DN45236_c1_g1_i2 from sample A8 / Example spliced isoform

GTGTCATGGACTTTGCTGTCTATCAATGGACAACACAGCAAACCAAACAGCCATCGTCTCTGAGCTGCTCCATAAAATTCTAACCCTAACCATCTTTTACTTTCCACCAACGCCGTGCATTTTCAATATGCCAAATCTTTCACTGCATGGATCTACTTCTTAACTCCATGCCTCCACGGCGGTTTGAACCACGGAATTTC
GGAATCCAGTTGGAGGATGCAAAAATCTCCAAAAATGGTGCGATACAGAGCTGTTTGTATGGAATGGGTAGAATTGAAAAGAAGAATAAGACTGGAAGGAACCGCACCAGTGGCCCAAAGTCCAGGCATTCTGTAGTGATTGTCTTAACCTTCAACAGGAGGGGAGGGTGTGGATGACCAGTTGTTAGGTTTTTGTCGAA
GAATGTAAGCAGTCTTCAACATCTGCCCACTTTTGAAATTGTATTTGCTACACTTCTTTCAAAAGTGTAGGAGATTCATAGAAAGATATGTAGTTTGGCCATGATAATACTTCTTTCAAAAGTGTAGGAGATTCATAGAAAGATATGTAGTTTGGCCATGATAATACTTCTTTCAAAAGTGTAGGAGAAATATGTAGTTT
GGCAATGATAACATGATTGTACCATAAATCGGAGGCGGTAGAGTTAGCTACATTTGGAAACCCACTAAACCATTGTATTCTGTTAAGCCCAGTTTTGATCTTTGAATGACTCGGAATGAATTATATATATATATATTGTATGCAGGCTGAGTGGATCGTCTAACGATTCACAACATATGTGTGTTCATATTTTGAGGTTA
GCCTCTATCTCAAAATTATCAATATAAAGAACCATTTGTCTATTTTAGAAATTATATATATACAGGAAAAATAAATAAGAAAATAAGAG

>TRINITY_DN45236_c1_g1_i1 from sample A8 / Example unspliced isoform:

GTGTCATGGACTTTGCTGTCTATCAATGGACAACACAGCAAACCAAACAGCCATCGTCTCTGAGCTGCTCCATAAAATTCTAACCCTAACCATCTTTTACTTTCCACCAACGCCGTGCATTTTCAATATGCCAAATCTTTCACTGCATGGATCTACTTCTTAACTCCATGCCTCCACGGCGGTTTGAACCACGGAATTTC
GGAATCCAGAGAATTACTATTTTTGTACAGTTGGAGGATGCAAAAATCTCCAAAAATGGTGCGATACAGAGCTGTTTGTATGGAATGGGTAGAATTGAAAAGAAGAATAAGACTGGAAGGAACCGgtatgtatgcatgtattctctgaaggccttgaaaccgtcttcagtgatcaatgtaacccatacttcttgtttaat
ttaatgaacgtttttttatctctgaataactcagaatgcgttctcttattgttgttaatggaaaacacttaacatagaaattactatacagCACCAGTGGCCCAAAGTCCAGGCATTCTGTAGTGATTGTCTTAACCTTCAACAGGAGGGGAGGGTGTGGATGACCAGTTGTTAGGTTTTTGTCGAAGAATGTAAGCAGT
CTTCAACATCTGCCCACTTTTGAAATTGTATTTGCTACACTTCTTTCAAAAGTGTAGGAGATTCATAGAAAGATATGTAGTTTGGCCATGATAATACTTCTTTCAAAAGTGTAGGAGATTCATAGAAAGATATGTAGTTTGGCCATGATAATACTTCTTTCAAAAGTGTAGGAGAAATATGTAGTTTGGCAATGATAACA
TGATTGTACCATAAATCGGAGGCGGTAGAGTTAGCTACATTTGGAAACCCACTAAACCATTGTATTCTGTTAAGCCCAGTTTTGATCTTTGAATGACTCGGAATGAATTATATATATATATATTGTATGCAGGCTGAGTGGATCGTCTAACGATTCACAACATATGTGTGTTCATATTTTGAGGTTAGCCTCTATCTCAA
AATTATCAATATAAAGAACCATTTGTCTATTTTAGAAATTATATATATACAGGAAAAATAAATAAGAAAATAAGAG

Intron 2 GTATGTATGC ... TACTATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	10	2
A2	71.43	3	6	9
A3	72.87	4	10.5	11
A4	90	1	9	9
A5	71.43	1	2	3
A6	50.15	83	50	117
A7	54.86	65	51	107
A8	52.11	91	64	134
A9	52.26	95	66	142
A10	63.37	37	52	76

>TRINITY_DN113383_c6_g1_i1 from sample A2 / Example spliced isoform

GAATAAGACTGGAAGGAACCGCACCAGTGGCCCAAAGTCCAGGCATTCTGTAGTGATTGTCTTAACCTTCAACAGGAGGGGAGGGTGTGGATGACCAGTTGTTAGGTTTTTGTCGAAGAATGTAAGCAGTCTTCAACATCTGCCCACTTTTGAAATTGTATTTGCTACACTTCTTTCAAAAGTGTAGGAGATTCATAGAA
AGATATGTAGTTTGGCCATGATAATACTTCTTTCAAAAGTGTAGGAGATTCATAGAAAAATATGTAGTTTGGCCATGATAACATGATTGTACCATAAATCGGAGGCAGTAGAGTTAGCTACATTTGGAAACCCACTAAACCATTGTATTCTGTTAAGCCCAGTTTTGATCTTTGAATGACTCGGAATGAATTATATATAT
ATATATTGTATGCAGGCTGAGTGGATCGTCTAACGATTCACAACATATGTGTGTTCATATTTTGAGGTTAGCCTCTATCTCAAAATTATC

>TRINITY_DN113383_c6_g1_i2 from sample A2 / Example unspliced isoform:

TTAGAAATCACAACAGAAAAATTTTATTTTTGAATTTTTTAAGGCTCCAGCATATAAAGTGTTTCTTGAATTTTCAAAGCTCTCGGTCTATGTACAGAGTCTCGAATCCTGAACCTTAACTGGAATCTCTAAAATCACGCATGCAAGCCAAGTGTCATGGCATGGGAATGCGAAGTGTCATGGACTTTGCTGTCTATCAA
TGGACAACACAGCAAACCAAACAGCCATCGTCTCTGAGCTGCTCCATAAAATTCTAACCCTAACCATCTTTTACTTTCCACCAACGCCGTGCATTTTCAATATGCCAAATCTTTCACTGCATGGATCTACTTCTTAACTCCATGCCTCCACGGCGGTTTGAACCACGGAATTTCGGAATCCAGAGAATTACTATTTTTGT
ACAGTTGGAGGATGCAAAAATCTCCAAAAATGGTGCGATACAGAGCTGTTTGTATGGAATGGGTAGAATTGAAAAGAAGAATAAGACTGGAAGGAACCGgtatgtatgcatgtattctctgaaggccttgaaaccgtcttcagtgatcaatgtaacccatatttcttgtttaatttaatgaactttttttatctctgaat
gactcagaatgcgttctcttattgttgttaatggaaaacacttaacatagaaattactatacagCACCAGTGGCCCAAAGTCCAGGCATTCTGTAGTGATTGTCTTAACCTTCAACAGGAGGGGAGGGTGTGGATGACCAGTTGTTAGGTTTTTGTCGAAGAATGTAAGCAGTCTTCAACATCTGCCCACTTTTGAAATT
GTATTTGCTACACTTCTTTCAAAAGTGTAGGAGATTCATAGAAAGATATGTAGTTTGGCCATGATAATACTTCTTTCAAAAGTGTAGGAGATTCATAGAAAAATATGTAGTTTGGCCATGATAACATGATTGTACCATAAATCGGAGGCAGTAGAGTTAGCTACATTTGGAAACCCACTAAACCATTGTATTCTGTTAAG
CCCAGTTTTGATCTTTGAATGACTCGGAATGAATTATATATATATATATTGTATGCAGGCTGAGTGGATCGTCTAACGATTCACAACATATGTGTGTTCATATTTTGAGGTTAGCCTCTATCTCAAAATTATC