NA ----> NA

Intron 1 GTTTGTGATG ... CGGATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.48	176.5	37	32.8
A2	10.03	138	17	14
A3	11.87	171.5	29	17.3
A4	14.85	84.5	15	14.5
A5	19.37	67	16	16.3
A6	6.97	266.5	24	16
A7	7.58	669	50	60
A8	8.1	666	68	49.8
A9	8.28	422	33	43.5
A10	5.88	541.5	40	27.8

>TRINITY_DN118781_c1_g1_i21 from sample A3 / Example spliced isoform

CACGGGGAGCGACATTTGCTATTATTGCTGGGAGAAGAAAAGATATAGAGGAGCCGAGCAGATAGAGGGTTTGTAAGATGGAGAGGTGGGAAGATTACAGTGTCTGCAATTATGGCAAATTCACGAGGATCCTCAGCAGATAGAGAGGAGCCGAGAAGATATACTAGACAGATATAGGGTTTGCAATTATGGGAGAAGAA
GGTTGAGGAGCCGAGAAGATATAGCAGAAAGATAGATAGTGTCTGCAATTATGGGAGAAGAAGGTTGAAGGTTGAATGATTTTTGGTTTGATTATGGTTTTGGATTTATGTTTTTCTCTTCAGAGCATGGCCATGCGTATTTGGTTTTATGTTTCATTCTGTGTTGATTTTTTTTCCTTTCCTTTTTTTATAATTCTTTT
ATTTTCGTTTTGGGTTTTGCTTTGTTGGGCTCTGTTTTTATAATTCTTTTATTTTCGTTTTGGGTTTTGCTTTGTTCGGCTCTGTTTTGAGTCTGGTTGGTTTGGGATCTAGAGGGAGGATGAAGGATTAAAATCCCAGAAGAAGTTGGCATCACTGGGTGGCCAACTGCAAAGGCGTAGAAGAGCCAGAGCCACAAGAC
CAAGACCAAGAGCCAGAGCAGAAATCTCAGTTGCGGCGGAGTTTCAGGGTTGGAGCTGAATGAAAGCTTCAAGTTAGTTTTGGGAGTGCATAAGTAAAGATAGAAAGCATTTTTGATGTGAACAGAAGAAAAAAATGTAACAGGTGAATGAAGAGTGCAAGTTATATTTGAAACTGTATCGACAGAATTAATATTTGAGT
GA

>TRINITY_DN118781_c1_g1_i8 from sample A3 / Example unspliced isoform:

CACGGGGAGCGACATTTGCTATTATTGCTGGGAGAAGAAAAGATATAGAGGAGCCGAGCAGATAGAGGGTTTGTAAGATGGAGAGGTGGGAAGATTACAGTGTCTGCAATTATGGCAAATTCACGAGGATCCTCAGCAGATAGAGAGGAGCCGAGAAGATATACTAGACAGATATAGGGTTTGCAATTATGGGAGAAGAA
GGTTGAGGAGCCGAGAAGATATAGCAGAAAGATAGATAGTGTCTGCAATTATGGGAGAAGAAGGTTGAAGGTTGAATGATTTTTGGTTTGATTATGGTTTTGGATTTATGTTTTTCTCTTCAGAGCATGGCCATGCGTATTTGGTTTTATGTTTCATTCTGTGTTGATTTTTTTTCCTTTCCTTTTTTTATAATTCTTTT
ATTTTCGTTTTGGGTTTTGCTTTGTTGGGCTCTGTTTTTATAATTCTTTTATTTTCGTTTTGGGTTTTGCTTTGTTCGGCTCTGTTTTGAGTCTGGTTGGTTTGGGATCTAGAGGGAGGATGAAGGATTAAAATCCCAGAAGAAGTTGGCATCACTGGGTGGCCAACTGCAAAGGCGTAGAAGgtttgtgatgtttaaat
ttctggatggacatttaatatatatattgcattatttcatcatgtaatgttttgtcccggattgcagAGCCAGAGCCACAAGACCAAGACCAAGAGCCAGAGCAGAAATCTCAGTTGCGGCGGAGTTTCAGGGTTGGAGCTGAATGAAAGCTTCAAGTTAGTTTTGGGAGTGCATAAGTAAAGATAGAAAGCATTTTTGA
TGTGAACAGAAGAAAAAAATGTAACAGGTGAATGAAGGCTACAGGTTATTTTTGGGAGTGCTGCATAAGAAAGACAAAGAACTTTGCAGGAGACTTTTGAGAGTGCTGCATAAGTAAAGACAAAGAACTTTGCAGGAGACTTTTGAGAGTGCTGCATAAGTAAAGACAAAGAACTTTGCAG

Intron 2 GTTTGTGCTC ... TGGAGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.86	70.2	86	37.4
A2	51.43	42.8	57.8	31.6
A3	53.43	41.8	54.6	40.6
A4	47.65	38.2	40.4	29
A5	61.19	14.8	26.6	19.8
A6	41.82	65.8	59.8	33.8
A7	31.09	253.4	158	66.2
A8	33.01	229.8	156	67
A9	30.9	128	75.6	37
A10	33.18	185.2	121.6	58.6

>TRINITY_DN122206_c0_g1_i15 from sample A2 / Example spliced isoform

AGATAAGAGAGTGGCGTTGAATAATTTTTGTGGTTTGATTATGGTTTTGTATTTGTGTCTTTTGTTTCGAGCATGGCCATGCTTATTTGGTTTTCCCTTTGATTTTGTCTTTCTTTTTTCTTTTTTTTATAATTCTTTTTCATTTTCATTTTTGGGTTTTGCTGTGTTCGTCTCTGTTTGTTTTGGGATCGAGAGGAGGA
TGACGTGGTTTGTTTGGGATTTTTCTTTAATAATACTTTCATTTTCGTTTTTGGGTTTTGCTTTGTTCGTCTTTGTTTTGAGTATGGTTTGTTTGGGATCGAGAGGAAGACGAGTGATTAGAAATCCAGAAGTTAGCATCACTGGGTAGCGAACTGCTAAGGCGGAGAAGAGGCAGAGCTACAAGACCAATAAATCAGAG
ATGCTGCGGAATTTCAAATCAGAGATGCTGCTGAATTTTGGGATTGGAGGTGGATAAAGAGTAAGATATTTTTGGGAGTGCATTATTGACATAATTAATATTTTGATAGAAATATAAAAAAAAGGTGGATAATGAGTGCATTGACAGAATTAATATTAACAAGTGGATAACCAATTCAAGTTATTTTCAATAATGCATTG
TCAGAATTAATATTTGAAAAAGAAGAGATGGAAAGAGCTATACAGGAGATTAACAATAGAAAATGTAAATATCTTTTTATCTAAACACTCAGAGATTATATTATTAATAAAATTATTATCTTTTCTGGGGAAGGGGCTTTCCCTTCTCAAATCCTTAAAGATAGCCAGCGACGGTTAGTTATCTTTGAGGAGTGTTTTCC
TGTGTTTTCTTTTGACAATGTTTTTCCTTCACATTTCC

>TRINITY_DN122206_c0_g1_i17 from sample A2 / Example unspliced isoform:

AGATAAGAGAGTGGCGTTGAATAATTTTTGTGGTTTGATTATGGTTTTGTATTTGTGTCTTTTGTTTCGAGCATGGCCATGCTTATTTGGTTTTCCCTTTGATTTTGTCTTTCTTTTTTCTTTTTTTTATAATTCTTTTTCATTTTCATTTTTGGGTTTTGCTGTGTTCGTCTCTGTTTGTTTTGGGATCGAGAGGAGGA
GGAAGTGGTTTGTTTGGGATTTTTCTTTAATAATACTTTCATTTTCGTTTTTGGGTTTTGCTTTGTTCGTCTCTGTTTTGAGTATGGTTTGTTTGGGATCGAGAGGAAGACGAGTGATTAGAAATCCAGAAGTTAGCATCACTGGGTAGCGAACTGCTAAGGCGGAGAAGgtttgtgctcttttatttcatgttaacatt
tctagatggaaatttaatccatattacataactacatcatgttttgttttgtcctggagtgcagAGGCAGAGCTACAAGACCAATAAATCAGAGATGCTGC