NA ----> NA

Intron 1 GTAAGTTTAC ... CTCTGAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.29	294	76	83
A2	19.28	224	67	40
A3	19.6	324	92	66
A4	20.06	247	50	74
A5	19.9	161	41	39
A6	18.34	69	16	15
A7	19.57	376	106	77
A8	21.03	107	32	25
A9	19.29	343	90	74
A10	20.93	529	150	130

>TRINITY_DN34051_c0_g1_i4 from sample A9 / Example spliced isoform

CTTTTATTTCATTCCTTTGTGTCCTAATTAGCAATCTCTTCATCTTCCTCTCTCTGATTCAGACATCTATCCCTCATATCTGAGACCCAGAATCTGCTCTGCTGCAATTCCTCTCGTATTGTTCAAGCACTTCACAAGAAAAGAAAAAGGGTCCCAAGTTCGCTGAGATATCAAGCTTTAGCAATAGTCATGGCGTTTCC
ATATCGGCCCAAGTTTCAATCTCCGAGGAAGCCCAGTTCTCCCTGCATTTCAAGGATCCATAATCCAAAGGAAAACAAAAATATCAACGACTGTTCTGAAGCCAGCAGGGCCGAAGATCGAGCCTCCTCGTTGAAGAAAGCAATGAAGAAAAGCTACGCCTCCCCTACGTTTGTGGCTTCTTCCAAGGCTAAAAAGGTAA
GACATGATCCTTCGTCATGGAAGCCCACTTGGGACAAAACACCTGTGGCCCCCAATTCTCCAGCCATGGCTACCTCACCGGTTAAAGATGCATTTACTGTAGCTAAGGAACAAAAGCTCAGCACGAGAGATGATGCCATGGAGGTGGAAAGAACGCCGGAGCCGCCAACTTTGGAGGAGTTGCAGAGAAAAGTTAAGAAG
TTCCTGGAAGAGCACACGCCCCAGCGCAGACCAGAAACCTCTGCTAATGCTATTTTATCTGAGGAAAAACAGAGTGGGATGCCACCTTACGATCCTAAGAAGAATTACTTGTCTCCTAGGCCGCAGTTTCTGCGTTATCGCCCTGGTAGAAATCTTGATTTTATTCTGAATGGTGAGAGCCCTGAGAGATTCCAACTGGA
AATTGACAAAGACCAAGCAAAGGAGCTTGGATGCTTGGATAAAAGTGTTTCAAAATCTGAGGATGTTGGAGTCAATGGCCTTCACTTTGAGGATGGATCTACAAAGAACAGGCCTAGGGTTGAGGATTTTCTGGATAGTGAGCCTCAACAAGGGAGTGAATTGCAGTCTTTGGAGAAGCAAATGGCTTCGGTTGACATAT
CAACAGGGATTGGATGTGAGCGTAAAGAAGGCTTTGGAGAGGATAAGCAATTTTGTGGATATGAAACATGTCAAGTTACAGATCAAGATTGCAAAGGTAGACAGCAGAGAAAAGATGAAGTACCTGTAGAAGAAACTTTGACCTGCAGCTCTATCTTTACTGAAACAGACAGAATTGAAGCTGTGATCAGATCGAGTACT
TCCATGTTTGAGCCCAAATCAGGTTCTGTTGTAGAGGTTGAAGAAAGTAGCGATGATGATGAAATGGAAGAAGCTCCCAAATGGATTCGTCTTGCGAAAAACTGCTTGATACTGATCTTTTCAGTGGTTTCTGCATTGGTGGTCATTCTAGCATCTGGTAGTCCAGCACTTCTTCCCTCTGAATTGCATGGCAATCCCTT
GGCTACTTCATGGCAGTCTAGCAAGTTATACAGTCCTACAATGAAGGTTTTGAGAGGTGCTGAGGGTCTTTGGAGCCCACCGATGTATTGTGATGTCAATTCACAAGCATTGCTGCAACATGGGCAGTCTGGGCTTAGGATGAGAACAAATGGTTTTTGGCTATCTGCGAAGGAATTTTGTTTGCAGCATTTTAAGAAGC
TTGGAGATGGTACTGAAAAGTTGCGAGGGTTTTCTGTCTCAGATTTCATTTCCATGCCTGTCGGTTTCAACGAACCTTCAGATGCTGAGGATTCAGGTCCTCCAATGGAATCTCATGATGAAAATCCAGAGGTCCCTGAAAAAAGTTTTTCACCCATACTCCCTTTCGAATCAAGTCAAATTGTGCACAGTGATGGGGCA
GAGTATGCTCATGACGACGAGTTTGGTTCCATGCGGTATAGTCTGAATGAACCTTCACTTGTTGAGGATTCAGTTTCTATGTTGGAACTGCATGATGAAAACCCATCCTATAGCCATGACAGACCTGGTAAAAGTGTTTTGCCCATACCCCCTTCTGGATTAAGTCAATTTGTGCAGAGTAATGGGGTACAGTATGGTCA
TGAAGCTGATTACTTCCATGACCAATTAAAGGATGGCAGTCATGAGGAGGAAGGTCACAATTATGAGCCTTCGGGCTTGTTCTCTGAAACCGAAGGCCAAGAGGATGCAGATAGATTTGATGGTTTATTTTCTCCTTTGCAAAAGCATCCTGAGGTGCCAATCTCATATGAGATCGGCTATTCATGGCTAGATGATATCG
GTGTTGGAACAGAAGGATTTGATATAATATATGGTGAAGAACAAACACCTCCTGCAGAGAACCAGCAGGTGATGGAAGTATCAGTTAATGCAGAAAATACCCAAGTTCTTGAATCTGTCATTTTAGACAATATATCAGATACAGAAAACTTGCCAGTAGATGCAGAAAAAAATGCCGATGGTTCTGAGGACAGTATTCCT
GAATTGGTATCAGTTATGGACATTGGTGCAGATATATCTCATGAACCTGAATCTCATTTTGGCTATAGTGGTTCAACTGACTTTGTTTTCTCCTCATCTGAAACAGAAACTAGCGGTGAAATACAGAGTTTTGAAGCTAACACAGAAGCAGTGCCTTTTGAGAGCCTTTTCATTGAAGATCCAGAGGAATTGGGCACATC
ATTTGATAAAGATGATCCACATATTCCTGCAATGGCCAGTCAGTCCAAGACACTAAATTTCGAGAGTCAAAATGAATTTGAATTCAGACATAATGAATCTGTCATGTCTCTAGTTGAGAGTAGTAGGGAGGGGGAATCAGACATATCAGTTGTTTATGATCTCGGAACTTCTAGTACTCCAACAGAATCAGTGAAATATA
TCTTGCTTTTTGGTACTATAGCTGCATCTATTGTTGCATCTGCGGTGCTTGCTACATATTTGGCTAGCGTGTACAGAAAGAATGACGGATGTGCAAGTCCTAAGAAAGCCCAAGAACACGATCAAAAACTTCTGGCAAAGAAAAATGGTCCTACAGAAGATGTTCATTCCATGCCAAGAAGGGAGAAATTATACCCTCAG
ACAAATCACTTTTCTTCAATGCGGATAACTGTGCCCTGCGAAAATAACTTCGAGCCAAGGGATTCCTATGTGCCCAGGGTGGAACTTCTTAACGAGTATTTGCCATCAAAGGTGGTAAGCACTTCAGAAAGAAAGCTCAATACCTGGCTTTTATCTAGTTCCCAAGAGAGTTCATTTCACACCCCGGAGAAGAAGGTTAG
GAGGAGAAACAACACATTCTCAGAAACAGAGCAAGGAAGTATGTCCACTCAAGAGATGTCCATGGATGATTCTCCATCTTATGGGAGCTTCACAACTTTGATGAAAGAGGTATTCTGCTTTTTTAATCTCAACAACCAACTTGTTATTGAGGATCCATATGAACCCAACGACCTTTTCCTTTGTCTTTTCTACACCCATG
TTTTTGTTTTGGACTAACATTCCAACTTTTACAAGGCATTTTCTTACTTATGAGTTGCCCTTACATTTGAAGTAAAAGTGGAATTTAGTTGCTTCATTTCGAGGTAGGTAGAAATACAGATTTTTTATGTCAGATTTTTTCAGTGCTATATGCGCAATTTTAAATACAGATGTTGTTCATGTGCTTATTGTAGTCTCAAT
CCTCTTTTCACTGCACCCTCTACCCTGTTTCATTATTATTTCTAGGATCTCTACACTCCCCCTGAAATTTTCTTCTCGGCAGTCTTCAATTCATTCCATGTGAAAGCAAATCAGCATGTAGGCAAGGTTTTATTTTGAGCTATAGATTGTGATCCAATTTCATACTTGTCTGGTTGTTTATCTCCAATTTTAAATACAGA
CGTTGTTCATGTGCTTATTCTAGTCTCAATCCTCTTTTTACTGCACCCTGCACCCTGCACCCTGTTTCATTATTATTTCTAAAATTCTAGGATCTCTACACTTCCCCCTGAAAATTTTCTTCTGGGTAGTCCTCAATTCATTCCATGTGAAAGCAAACCAGCATGTAGGCAATGTTTTACTTTGAGCTATATAGACTGTG
TTCCAATTTCATACTTGTCTGGTTGTTTATCTCCACTTTTTTTTATCAGAGAGACATTTTTATGGGTACTTTGTGTCTACAATGCAGGGTAGAGATGAAGAGCAGATGAAAGTAACACCTGTAAGAAGATCAAACCGACTTCGTAACCAAAAGAAGTCTCCATTATCAGCTATACAAACGATCTCTAAGTGAAGATCCTG
CATCACGTTCTTCCTTAGACCTACCTGACCTGAGCACACAGCAAAGCATGAATGAGAAAGAGAATTTGTTCTTCAAAATTTGTGGCTGTCAATCACCAACCTCCAACAAGATATTTTGTTATGGGTGATCTTGAATTTTGAGATAGAGCATATTTTAAGGATGGGGTTTTGCATTTCTGGATAGCGAGGCGCACACCCGG
AGGCCTTTTGTTCTGATGTTGAATTCTTATGATATATGCTTTTAATTGCCACCTCCTTTAAACACAATCAATAGTTGTTTCAGTTTTTAATCAGATGTATATACATACTTGTATTTATTCATCAGATCCTACACCTATTGGAGAGAAATCAAGGCCAGCTCGTATAATTCTTTTTGATAGTAGTCACTGTCTATTTCGCT
TTTGCCTTTGATATTGAGAATACAGCTAGTGGCTTTGCAATCTTTGTCAATCATTACTCATGCAAAAACTGGAAATACATTAAAGTCTAGTTCAGTTCTTGGGATAGACATGCTACTCAATCTTTGCAGTCTTAAAAGGTGGATTATATCTTTCAACTATTTATTTGGACATTGATAACATGCAAATATGGG

>TRINITY_DN34051_c0_g1_i3 from sample A9 / Example unspliced isoform:

CTTTTATTTCATTCCTTTGTGTCCTAATTAGCAATCTCTTCATCTTCCTCTCTCTGATTCAGACATCTATCCCTCATATCTGAGACCCAGAATCTGCTCTGCTGCAATTCCTCTCGTATTGTTCAAGCACTTCACAAGAAAAGAAAAAGGGTCCCAAGTTCGCTGAGATATCAAGCTTTAGCAATAGTCATGGCGTTTCC
ATATCGGCCCAAGTTTCAATCTCCGAGGAAGCCCAGTTCTCCCTGCATTTCAAGGATCCATAATCCAAAGGAAAACAAAAATATCAACGACTGTTCTGAAGCCAGCAGGGCCGAAGATCGAGCCTCCTCGTTGAAGAAAGCAATGAAGAAAAGCTACGCCTCCCCTACGTTTGTGGCTTCTTCCAAGGCTAAAAAGGTAA
GACATGATCCTTCGTCATGGAAGCCCACTTGGGACAAAACACCTGTGGCCCCCAATTCTCCAGCCATGGCTACCTCACCGGTTAAAGATGCATTTACTGTAGCTAAGGAACAAAAGCTCAGCACGAGAGATGATGCCATGGAGGTGGAAAGAACGCCGGAGCCGCCAACTTTGGAGGAGTTGCAGAGAAAAGTTAAGAAG
TTCCTGGAAGAGCACACGCCCCAGCGCAGACCAGAAACCTCTGCTAATGCTATTTTATCTGAGgtaagtttacattcttccccttatcttcacattcattcagattttgtatatatgggtagtcctccacattggccttcatgtgatgtaataaacatctctgagcagGAAAAACAGAGTGGGATGCCACCTTACGATCC
TAAGAAGAATTACTTGTCTCCTAGGCCGCAGTTTCTGCGTTATCGCCCTGGTAGAAATCTTGATTTTATTCTGAATGGTGAGAGCCCTGAGAGATTCCAACTGGAAATTGACAAAGACCAAGCAAAGGAGCTTGGATGCTTGGATAAAAGTGTTTCAAAATCTGAGGATGTTGGAGTCAATGGCCTTCACTTTGAGGATG
GATCTACAAAGAACAGGCCTAGGGTTGAGGATTTTCTGGATAGTGAGCCTCAACAAGGGAGTGAATTGCAGTCTTTGGAGAAGCAAATGGCTTCGGTTGACATATCAACAGGGATTGGATGTGAGCGTAAAGAAGGCTTTGGAGAGGATAAGCAATTTTGTGGATATGAAACATGTCAAGTTACAGATCAAGATTGCAAA
GGTAGACAGCAGAGAAAAGATGAAGTACCTGTAGAAGAAACTTTGACCTGCAGCTCTATCTTTACTGAAACAGACAGAATTGAAGCTGTGATCAGATCGAGTACTTCCATGTTTGAGCCCAAATCAGGTTCTGTTGTAGAGGTTGAAGAAAGTAGCGATGATGATGAAATGGAAGAAGCTCCCAAATGGATTCGTCTTGC
GAAAAACTGCTTGATACTGATCTTTTCAGTGGTTTCTGCATTGGTGGTCATTCTAGCATCTGGTAGTCCAGCACTTCTTCCCTCTGAATTGCATGGCAATCCCTTGGCTACTTCATGGCAGTCTAGCAAGTTATACAGTCCTACAATGAAGGTTTTGAGAGGTGCTGAGGGTCTTTGGAGCCCACCGATGTATTGTGATG
TCAATTCACAAGCATTGCTGCAACATGGGCAGTCTGGGCTTAGGATGAGAACAAATGGTTTTTGGCTATCTGCGAAGGAATTTTGTTTGCAGCATTTTAAGAAGCTTGGAGATGGTACTGAAAAGTTGCGAGGGTTTTCTGTCTCAGATTTCATTTCCATGCCTGTCGGTTTCAACGAACCTTCAGATGCTGAGGATTCA
GGTCCTCCAATGGAATCTCATGATGAAAATCCAGAGGTCCCTGAAAAAAGTTTTTCACCCATACTCCCTTTCGAATCAAGTCAAATTGTGCACAGTGATGGGGCAGAGTATGCTCATGACGACGAGTTTGGTTCCATGCGGTATAGTCTGAATGAACCTTCACTTGTTGAGGATTCAGTTTCTATGTTGGAACTGCATGA
TGAAAACCCATCCTATAGCCATGACAGACCTGGTAAAAGTGTTTTGCCCATACCCCCTTCTGGATTAAGTCAATTTGTGCAGAGTAATGGGGTACAGTATGGTCATGAAGCTGATTACTTCCATGACCAATTAAAGGATGGCAGTCATGAGGAGGAAGGTCACAATTATGAGCCTTCGGGCTTGTTCTCTGAAACCGAAG
GCCAAGAGGATGCAGATAGATTTGATGGTTTATTTTCTCCTTTGCAAAAGCATCCTGAGGTGCCAATCTCATATGAGATCGGCTATTCATGGCTAGATGATATCGGTGTTGGAACAGAAGGATTTGATATAATATATGGTGAAGAACAAACACCTCCTGCAGAGAACCAGCAGGTGATGGAAGTATCAGTTAATGCAGAA
AATACCCAAGTTCTTGAATCTGTCATTTTAGACAATATATCAGATACAGAAAACTTGCCAGTAGATGCAGAAAAAAATGCCGATGGTTCTGAGGACAGTATTCCTGAATTGGTATCAGTTATGGACATTGGTGCAGATATATCTCATGAACCTGAATCTCATTTTGGCTATAGTGGTTCAACTGACTTTGTTTTCTCCTC
ATCTGAAACAGAAACTAGCGGTGAAATACAGAGTTTTGAAGCTAACACAGAAGCAGTGCCTTTTGAGAGCCTTTTCATTGAAGATCCAGAGGAATTGGGCACATCATTTGATAAAGATGATCCACATATTCCTGCAATGGCCAGTCAGTCCAAGACACTAAATTTCGAGAGTCAAAATGAATTTGAATTCAGACATAATG
AATCTGTCATGTCTCTAGTTGAGAGTAGTAGGGAGGGGGAATCAGACATATCAGTTGTTTATGATCTCGGAACTTCTAGTACTCCAACAGAATCAGTGAAATATATCTTGCTTTTTGGTACTATAGCTGCATCTATTGTTGCATCTGCGGTGCTTGCTACATATTTGGCTAGCGTGTACAGAAAGAATGACGGATGTGCA
AGTCCTAAGAAAGCCCAAGAACACGATCAAAAACTTCTGGCAAAGAAAAATGGTCCTACAGAAGATGTTCATTCCATGCCAAGAAGGGAGAAATTATACCCTCAGACAAATCACTTTTCTTCAATGCGGATAACTGTGCCCTGCGAAAATAACTTCGAGCCAAGGGATTCCTATGTGCCCAGGGTGGAACTTCTTAACGA
GTATTTGCCATCAAAGGTGGTAAGCACTTCAGAAAGAAAGCTCAATACCTGGCTTTTATCTAGTTCCCAAGAGAGTTCATTTCACACCCCGGAGAAGAAGGTTAGGAGGAGAAACAACACATTCTCAGAAACAGAGCAAGGAAGTATGTCCACTCAAGAGATGTCCATGGATGATTCTCCATCTTATGGGAGCTTCACAA
CTTTGATGAAAGAGGTATTCTGCTTTTTTAATCTCAACAACCAACTTGTTATTGAGGATCCATATGAACCCAACGACCTTTTCCTTTGTCTTTTCTACACCCATGTTTTTGTTTTGGACTAACATTCCAACTTTTACAAGGCATTTTCTTACTTATGAGTTGCCCTTACATTTGAAGTAAAAGTGGAATTTAGTTGCTTC
ATTTCGAGGTAGGTAGAAATACAGATTTTTTATGTCAGATTTTTTCAGTGCTATATGCGCAATTTTAAATACAGATGTTGTTCATGTGCTTATTGTAGTCTCAATCCTCTTTTCACTGCACCCTCTACCCTGTTTCATTATTATTTCTAGGATCTCTACACTCCCCCTGAAATTTTCTTCTCGGCAGTCTTCAATTCATT
CCATGTGAAAGCAAATCAGCATGTAGGCAAGGTTTTATTTTGAGCTATAGATTGTGATCCAATTTCATACTTGTCTGGTTGTTTATCTCCAATTTTAAATACAGACGTTGTTCATGTGCTTATTCTAGTCTCAATCCTCTTTTTACTGCACCCTGCACCCTGCACCCTGTTTCATTATTATTTCTAAAATTCTAGGATCT
CTACACTTCCCCCTGAAAATTTTCTTCTGGGTAGTCCTCAATTCATTCCATGTGAAAGCAAACCAGCATGTAGGCAATGTTTTACTTTGAGCTATATAGACTGTGTTCCAATTTCATACTTGTCTGGTTGTTTATCTCCACTTTTTTTTATCAGAGAGACATTTTTATGGGTACTTTGTGTCTACAATGCAGGGTAGAGA
TGAAGAGCAGATGAAAGTAACACCTGTAAGAAGATCAAACCGACTTCGTAACCAAAAGAAGTCTCCATTATCAGCTATACAAACGATCTCTAAGTGAAGATCCTGCATCACGTTCTTCCTTAGACCTACCTGACCTGAGCACACAGCAAAGCATGAATGAGAAAGAGAATTTGTTCTTCAAAATTTGTGGCTGTCAATCA
CCAACCTCCAACAAGATATTTTGTTATGGGTGATCTTGAATTTTGAGATAGAGCATATTTTAAGGATGGGGTTTTGCATTTCTGGATAGCGAGGCGCACACCCGGAGGCCTTTTGTTCTGATGTTGAATTCTTATGATATATGCTTTTAATTGCCACCTCCTTTAAACACAATCAATAGTTGTTTCAGTTTTTAATCAGA
TGTATATACATACTTGTATTTATTCATCAGATCCTACACCTATTGGAGAGAAATCAAGGCCAGCTCGTATAATTCTTTTTGATAGTAGTCACTGTCTATTTCGCTTTTGCCTTTGATATTGAGAATACAGCTAGTGGCTTTGCAATCTTTGTCAATCATTACTCATGCAAAAACTGGAAATACATTAAAGTCTAGTTCAG
TTCTTGGGATAGACATGCTACTCAATCTTTGCAGTCTTAAAAGGTGGATTATATCTTTCAACTATTTATTTGGACATTGATAACATGCAAATATGGG

Intron 2 GTATTCTGCT ... TACAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.61	400	130	41
A2	18.53	288	98	33
A3	17.84	357	102	53
A4	20.3	214	92	17
A5	18.88	101	40	7
A6	18.49	637	201	88
A7	17.94	2274	692	302
A8	17.15	2674	716	391
A9	18.95	2004	669	268
A10	18.98	1804	585	260

>TRINITY_DN35085_c0_g2_i2 from sample A7 / Example spliced isoform

ATTGCTAAAACCGTGCTTCTCTACCTCGCTTTTATTTCATTCCTTTGTGTCCTAATTAGCAATCTCTTCATCTTCCTCTCTCTGATTCAGACATCTATCCCTCATATCTGAGACCCAGAATCTGCTCTGCTGCAATTCCTCTCGTATTGTTCAAGCACTTCACAAGAAAAGAAAAAGGGTCCCAAGTTCGCTGAGATATC
AAGCTTTAGCAATAGTCATGGCGTTTCCATATCGGCCCAAGTTTCAATCTCCGAGGAAGCCCAGTTCTCCCTGCATTTCAAGGATCCATAATCCAAAGGAAAACAAAAATATCAACGACTGTTCTGAAGCCAGCAGGGCCGAAGATCGAGCCTCCTCGTTGAAGAAAGCAATGAAGAAAAGCTACGCCTCCCCTACGTTT
GTGGCTTCTTCCAAGGCTAAAAAGGTAAGACATGATCCTTCGTCATGGAAGCCCACTTGGGACAAAACACCTGTGGCCCCCAATTCTCCAGCCATGGCTACCTCACCGGTTAAAGATGCATTTACTGTAGCTAAGGAACAAAAGCTCAGCACGAGAGATGATGCCATGGAGGTGGAAAGAACGCCGGAGCCGCCAACTTT
GGAGGAGTTGCAGAGAAAAGTTAAGAAGTTCCTGGAAGAGCACACGCCCCAGCGCAGACCAGAAACCTCTGCTAATGCTATTTTATCTGAGGAAAAACAGAGTGGGATGCCACCTTACGATCCTAAGAAGAATTACTTGTCTCCTAGGCCGCAGTTTCTGCGTTATCGCCCTGGTAGAAATCTTGATTTTATTCTGAATG
GTGAGAGCCCTGAGAGATTCCAACTGGAAATTGACAAAGACCAAGCAAAGGAGCTTGGATGCTTGGATAAAAGTGTTTCAAAATCTGAGGATGTTGGAGTCAATGGCCTTCACTTTGAGGATGGATCTACAAAGAACAGGCCTAGGGTTGAGGATTTTCTGGATAGTGAGCCTCAACAAGGGAGTGAATTGCAGTCTTTG
GAGAAGCAAATGGCTTCGGTTGACATATCAACAGGGATTGGATGTGAGCGTAAAGAAGGCTTTGGAGAGGATAAGCAATTTTGTGGATATGAAACATGTCAAGTTACAGATCAAGATTGCAAAGGTAGACAGCAGAGAAAAGATGAAGTACCTGTAGAAGAAACTTTGACCTGCAGCTCTATCTTTACTGAAACAGACAG
AATTGAAGCTGTGATCAGATCGAGTACTTCCATGTTTGAGCCCAAATCAGGTTCTGTTGTAGAGGTTGAAGAAAGTAGCGATGATGATGAAATGGAAGAAGCTCCCAAATGGATTCGTCTTGCGAAAAACTGCTTGATACTGATCTTTTCAGTGGTTTCTGCATTGGTGGTCATTCTAGCATCTGGTAGTCCAGCACTTC
TTCCCTCTGAATTGCATGGCAATCCCTTGGCTACTTCATGGCAGTCTAGCAAGTTATACAGTCCTACAATGAAGGTTTTGAGAGGTGCTGAGGGTCTTTGGAGCCCACCGATGTATTGTGATGTCAATTCACAAGCATTGCTGCAACATGGGCAGTCTGGGCTTAGGATGAGAACAAATGGTTTTTGGCTATCTGCGAAG
GAATTTTGTTTGCAGCATTTTAAGAAGCTTGGAGATGGTACTGAAAAGTTGCGAGGGTTTTCTGTCTCAGATTTCATTTCCATGCCTGTCGGTTTCAACGAACCTTCAGATGCTGAGGATTCAGGTCCTCCAATGGAATCTCATGATGAAAATCCAGAGGTCCCTGAAAAAAGTTTTTCACCCATACTCCCTTTCGAATC
AAGTCAAATTGTGCACAGTGATGGGGCAGAGTATGCTCATGACGACGAGTTTGGTTCCATGCGGTATAGTCTGAATGAACCTTCACTTGTTGAGGATTCAGTTTCTATGTTGGAACTGCATGATGAAAACCCATCCTATAGCCATGACAGACCTGGTAAAAGTGTTTTGCCCATACCCCCTTCTGGATTAAGTCAATTTG
TGCAGAGTAATGGGGTACAGTATGGTCATGAAGCTGATTACTTCCATGACCAATTAAAGGATGGCAGTCATGAGGAGGAAGGTCACAATTATGAGCCTTCGGGCTTGTTCTCTGAAACCGAAGGCCAAGAGGATGCAGATAGATTTGATGGTTTATTTTCTCCTTTGCAAAAGCATCCTGAGGTGCCAATCTCATATGAG
ATCGGCTATTCATGGCTAGATGATATCGGTGTTGGAACAGAAGGATTTGATATAATATATGGTGAAGAACAAACACCTCCTGCAGAGAACCAGCAGGTGATGGAAGTATCAGTTAATGCAGAAAATACCCAAGTTCTTGAATCTGTCATTTTAGACAATATATCAGATACAGAAAACTTGCCAGTAGATGCAGAAAAAAA
TGCCGATGGTTCTGAGGACAGTATTCCTGAATTGGTATCAGTTATGGACATTGGTGCAGATATATCTCATGAACCTGAATCTCATTTTGGCTATAGTGGTTCAACTGACTTTGTTTTCTCCTCATCTGAAACAGAAACTAGCGGTGAAATACAGAGTTTTGAAGCTAACACAGAAGCAGTGCCTTTTGAGAGCCTTTTCA
TTGAAGATCCAGAGGAATTGGGCACATCATTTGATAAAGATGATCCACATATTCCTGCAATGGCCAGTCAGTCCAAGACACTAAATTTCGAGAGTCAAAATGAATTTGAATTCAGACATAATGAATCTGTCATGTCTCTAGTTGAGAGTAGTAGGGAGGGGGAATCAGACATATCAGTTGTTTATGATCTCGGAACTTCT
AGTACTCCAACAGAATCAGTGAAATATATCTTGCTTTTTGGTACTATAGCTGCATCTATTGTTGCATCTGCGGTGCTTGCTACATATTTGGCTAGCGTGTACAGAAAGAATGACGGATGTGCAAGTCCTAAGAAAGCCCAAGAACACGATCAAAAACTTCTGGCAAAGAAAAATGGTCCTACAGAAGATGTTCATTCCAT
GCCAAGAAGGGAGAAATTATACCCTCAGACAAATCACTTTTCTTCAATGCGGATAACTGTGCCCTGCGAAAATAACTTCGAGCCAAGGGATTCCTATGTGCCCAGGGTGGAACTTCTTAACGAGTATTTGCCATCAAAGGTGGTAAGCACTTCAGAAAGAAAGCTCAATACCTGGCTTTTATCTAGTTCCCAAGAGAGTT
CATTTCACACCCCGGAGAAGAAGGTTAGGAGGAGAAACAACACATTCTCAGAAACAGAGCAAGGAAGTATGTCCACTCAAGAGATGTCCATGGATGATTCTCCATCTTATGGGAGCTTCACAACTTTGATGAAAGAGGGTAGAGATGAAGAGCAGATGAAAGTAACACCTGTAAGAAGATCAAACCGACTTCGTAACCAA
AAGAAGTCTCCATTATCAGCTATACAAACGATCTCTAAGTGAAGATCCTGCATCACGTTCTTCCTTAGACCTACCTGACCTGAGCACACAGCAAAGCATGAATGAGAAAGAGAATTTGTTCTTCAAAATTTGTGGCTGTCAATCACCAACCTCCAACAAGATATTTTGTTATGGGTGATCTTGAATTTTGAGATAGAGCA
TATTTTAAGGATGGGGTTTTGCATTTCTGGATAGCGAGGCGCACACCCGGAGGCCTTTTGTTCTGATGTTGAATTCTTATGATATATGCTTTTAATTGCCACCTCCTTTAAACACAATCAATAGTTGTTTCAGTTTTTAATCAGATGTATATACATACTTGTATTTATTCATCAGATCCTACACCTATTGGAGAGAAATC
AAGGCCAGCTCGTATAATTCTTTTTGATAGTAGTCACTGTCTATTTCGCTTTTGCCTTTGATATTGAGAATACAGCTAGTGGCTTTGCAATCTTTGTCAATCATTACTCATGCAAAAACTGGAAATACATTAAAGTCTAGTTCAGTTCTTGGGATAGACATGCTACTCAAA

>TRINITY_DN35085_c0_g2_i1 from sample A7 / Example unspliced isoform:

ATTGCTAAAACCGTGCTTCTCTACCTCGCTTTTATTTCATTCCTTTGTGTCCTAATTAGCAATCTCTTCATCTTCCTCTCTCTGATTCAGACATCTATCCCTCATATCTGAGACCCAGAATCTGCTCTGCTGCAATTCCTCTCGTATTGTTCAAGCACTTCACAAGAAAAGAAAAAGGGTCCCAAGTTCGCTGAGATATC
AAGCTTTAGCAATAGTCATGGCGTTTCCATATCGGCCCAAGTTTCAATCTCCGAGGAAGCCCAGTTCTCCCTGCATTTCAAGGATCCATAATCCAAAGGAAAACAAAAATATCAACGACTGTTCTGAAGCCAGCAGGGCCGAAGATCGAGCCTCCTCGTTGAAGAAAGCAATGAAGAAAAGCTACGCCTCCCCTACGTTT
GTGGCTTCTTCCAAGGCTAAAAAGGTAAGACATGATCCTTCGTCATGGAAGCCCACTTGGGACAAAACACCTGTGGCCCCCAATTCTCCAGCCATGGCTACCTCACCGGTTAAAGATGCATTTACTGTAGCTAAGGAACAAAAGCTCAGCACGAGAGATGATGCCATGGAGGTGGAAAGAACGCCGGAGCCGCCAACTTT
GGAGGAGTTGCAGAGAAAAGTTAAGAAGTTCCTGGAAGAGCACACGCCCCAGCGCAGACCAGAAACCTCTGCTAATGCTATTTTATCTGAGGAAAAACAGAGTGGGATGCCACCTTACGATCCTAAGAAGAATTACTTGTCTCCTAGGCCGCAGTTTCTGCGTTATCGCCCTGGTAGAAATCTTGATTTTATTCTGAATG
GTGAGAGCCCTGAGAGATTCCAACTGGAAATTGACAAAGACCAAGCAAAGGAGCTTGGATGCTTGGATAAAAGTGTTTCAAAATCTGAGGATGTTGGAGTCAATGGCCTTCACTTTGAGGATGGATCTACAAAGAACAGGCCTAGGGTTGAGGATTTTCTGGATAGTGAGCCTCAACAAGGGAGTGAATTGCAGTCTTTG
GAGAAGCAAATGGCTTCGGTTGACATATCAACAGGGATTGGATGTGAGCGTAAAGAAGGCTTTGGAGAGGATAAGCAATTTTGTGGATATGAAACATGTCAAGTTACAGATCAAGATTGCAAAGGTAGACAGCAGAGAAAAGATGAAGTACCTGTAGAAGAAACTTTGACCTGCAGCTCTATCTTTACTGAAACAGACAG
AATTGAAGCTGTGATCAGATCGAGTACTTCCATGTTTGAGCCCAAATCAGGTTCTGTTGTAGAGGTTGAAGAAAGTAGCGATGATGATGAAATGGAAGAAGCTCCCAAATGGATTCGTCTTGCGAAAAACTGCTTGATACTGATCTTTTCAGTGGTTTCTGCATTGGTGGTCATTCTAGCATCTGGTAGTCCAGCACTTC
TTCCCTCTGAATTGCATGGCAATCCCTTGGCTACTTCATGGCAGTCTAGCAAGTTATACAGTCCTACAATGAAGGTTTTGAGAGGTGCTGAGGGTCTTTGGAGCCCACCGATGTATTGTGATGTCAATTCACAAGCATTGCTGCAACATGGGCAGTCTGGGCTTAGGATGAGAACAAATGGTTTTTGGCTATCTGCGAAG
GAATTTTGTTTGCAGCATTTTAAGAAGCTTGGAGATGGTACTGAAAAGTTGCGAGGGTTTTCTGTCTCAGATTTCATTTCCATGCCTGTCGGTTTCAACGAACCTTCAGATGCTGAGGATTCAGGTCCTCCAATGGAATCTCATGATGAAAATCCAGAGGTCCCTGAAAAAAGTTTTTCACCCATACTCCCTTTCGAATC
AAGTCAAATTGTGCACAGTGATGGGGCAGAGTATGCTCATGACGACGAGTTTGGTTCCATGCGGTATAGTCTGAATGAACCTTCACTTGTTGAGGATTCAGTTTCTATGTTGGAACTGCATGATGAAAACCCATCCTATAGCCATGACAGACCTGGTAAAAGTGTTTTGCCCATACCCCCTTCTGGATTAAGTCAATTTG
TGCAGAGTAATGGGGTACAGTATGGTCATGAAGCTGATTACTTCCATGACCAATTAAAGGATGGCAGTCATGAGGAGGAAGGTCACAATTATGAGCCTTCGGGCTTGTTCTCTGAAACCGAAGGCCAAGAGGATGCAGATAGATTTGATGGTTTATTTTCTCCTTTGCAAAAGCATCCTGAGGTGCCAATCTCATATGAG
ATCGGCTATTCATGGCTAGATGATATCGGTGTTGGAACAGAAGGATTTGATATAATATATGGTGAAGAACAAACACCTCCTGCAGAGAACCAGCAGGTGATGGAAGTATCAGTTAATGCAGAAAATACCCAAGTTCTTGAATCTGTCATTTTAGACAATATATCAGATACAGAAAACTTGCCAGTAGATGCAGAAAAAAA
TGCCGATGGTTCTGAGGACAGTATTCCTGAATTGGTATCAGTTATGGACATTGGTGCAGATATATCTCATGAACCTGAATCTCATTTTGGCTATAGTGGTTCAACTGACTTTGTTTTCTCCTCATCTGAAACAGAAACTAGCGGTGAAATACAGAGTTTTGAAGCTAACACAGAAGCAGTGCCTTTTGAGAGCCTTTTCA
TTGAAGATCCAGAGGAATTGGGCACATCATTTGATAAAGATGATCCACATATTCCTGCAATGGCCAGTCAGTCCAAGACACTAAATTTCGAGAGTCAAAATGAATTTGAATTCAGACATAATGAATCTGTCATGTCTCTAGTTGAGAGTAGTAGGGAGGGGGAATCAGACATATCAGTTGTTTATGATCTCGGAACTTCT
AGTACTCCAACAGAATCAGTGAAATATATCTTGCTTTTTGGTACTATAGCTGCATCTATTGTTGCATCTGCGGTGCTTGCTACATATTTGGCTAGCGTGTACAGAAAGAATGACGGATGTGCAAGTCCTAAGAAAGCCCAAGAACACGATCAAAAACTTCTGGCAAAGAAAAATGGTCCTACAGAAGATGTTCATTCCAT
GCCAAGAAGGGAGAAATTATACCCTCAGACAAATCACTTTTCTTCAATGCGGATAACTGTGCCCTGCGAAAATAACTTCGAGCCAAGGGATTCCTATGTGCCCAGGGTGGAACTTCTTAACGAGTATTTGCCATCAAAGGTGGTAAGCACTTCAGAAAGAAAGCTCAATACCTGGCTTTTATCTAGTTCCCAAGAGAGTT
CATTTCACACCCCGGAGAAGAAGGTTAGGAGGAGAAACAACACATTCTCAGAAACAGAGCAAGGAAGTATGTCCACTCAAGAGATGTCCATGGATGATTCTCCATCTTATGGGAGCTTCACAACTTTGATGAAAGAGgtattctgcttttttaatctcaacaaccaacttgttattgaggatccatatgaacccaacgac
cttttcctttgtcttttctacacccatgtttttgttttggactaacattccaacttttacaaggcattttcttacttatgagttgcccttacatttgaagtaaaagtggaatttagttgcttcatttcgaggtaggtagaaatacagattttttatgtcagattttttcagtgctatatgcgcaattttaaatacagatg
ttgttcatgtgcttattgtagtctcaatcctcttttcactgcaccctctaccctgtttcattattatttctaggatctctacactccccctgaaattttcttctcggcagtcttcaattcattccatgtgaaagcaaatcagcatgtaggcaaggttttattttgagctatagattgtgatccaatttcatacttgtctg
gttgtttatctccaattttaaatacagacgttgttcatgtgcttattctagtctcaatcctctttttactgcaccctgcaccctgcaccctgtttcattattatttctaaaattctaggatctctacacttccccctgaaaattttcttctgggtagtcctcaattcattccatgtgaaagcaaaccagcatgtaggcaa
tgttttactttgagctatatagactgtgttccaatttcatacttgtctggttgtttatctccacttttttttatcagagagacatttttatgggtactttgtgtctacaatgcagGGTAGAGATGAAGAGCAGATGAAAGTAACACCTGTAAGAAGATCAAACCGACTTCGTAACCAAAAGAAGTCTCCATTATCAGCTA
TACAAACGATCTCTAAGTGAAGATCCTGCATCACGTTCTTCCTTAGACCTACCTGACCTGAGCACACAGCAAAGCATGAATGAGAAAGAGAATTTGTTCTTCAAAATTTGTGGCTGTCAATCACCAACCTCCAACAAGATATTTTGTTATGGGTGATCTTGAATTTTGAGATAGAGCATATTTTAAGGATGGGGTTTTGC
ATTTCTGGATAGCGAGGCGCACACCCGGAGGCCTTTTGTTCTGATGTTGAATTCTTATGATATATGCTTTTAATTGCCACCTCCTTTAAACACAATCAATAGTTGTTTCAGTTTTTAATCAGATGTATATACATACTTGTATTTATTCATCAGATCCTACACCTATTGGAGAGAAATCAAGGCCAGCTCGTATAATTCTT
TTTGATAGTAGTCACTGTCTATTTCGCTTTTGCCTTTGATATTGAGAATACAGCTAGTGGCTTTGCAATCTTTGTCAATCATTACTCATGCAAAAACTGGAAATACATTAAAGTCTAGTTCAGTTCTTGGGATAGACATGCTACTCAAA