NA ----> NA

Intron 1 GTACTTTCCC ... ATTCCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.49	207	108	3
A2	29.35	206	108	5.7
A3	30.23	305	168.7	1
A4	30.53	206	116	4
A5	28.9	115	58.7	1.7
A6	30.33	1479	818.7	3
A7	30.2	1797	992	6
A8	30.15	1609	885.3	5
A9	30.03	2151	1175.3	8
A10	30.05	1687	922.7	5

>TRINITY_DN91282_c0_g1_i3 from sample A5 / Example spliced isoform

GGTATCAGTCAGAGCAAGCTTGATAAACCAATAATTATTTATTAAATAGTAAATACCGAGGAAAGGAAGCATAAGACGAAGAGCGAAGGCAAAGAGAACAGCAGGCACAAGGTGAGATATGGCGAACGCAACAGCAGCCTCAAATGCTCTGAGGAGAGGTCTCAACCTTCTGGCCACATCAAATGCTGCTCTCCGCCGTC
CAGTCGGCCACATTAGAGGCACTGTACTGTAATATTGCTTCAAATGGGTCATACATTCACAATAATCCACCCAGCTTCCGCCAACGATATTTATCGATCACATGGTCTATGTAAATATGGAAGAATGGCTAGGGTTTTTAGGGTTGCTATCTTCTCTTGAGGTTGTCTGGTCATTCTTTTTTATCATGCTAATTTCTATT
ATCTATGCTATTACGGACAAATCCTCTGAAAATAACTGGTGAAAGTTCTCCATGACGATGATCTGCTGTCTTCCTAGAGTTTATTATTCACTTAAACGAGATACGACTATAAGAGATCCGAG

>TRINITY_DN91282_c0_g1_i1 from sample A5 / Example unspliced isoform:

AAATGCTGCTCTCCGCCGTCCAGgtactttcccctctctctctatctttaaatcttcttttctgctagggtttgttatttaattcacgaagttccgtttcaattcctgcagTCGGCCACATTAGAGGCACTGTACTGTAATATTGCTTCAAATGGGTCATACATTCACAATAATCCACCCAGCTTCCGCCAACGATATTT
ATCGATCACATGGTCTATGTAAATATGGAAGAATGGCTAGGGTTTTTAGGGTTGCTATCTTCTCTTGAGGTTGTCTGGTCATTCTTTTTTATCATGCTAATTTCTATTATCTATGCTATTACGGACAAATCCTCTGAAAATAACTGGTGAAAGTTCTCCATGACGATGATCTGCTGTCTTCCTAGAGTTTATTATTCACT
TAAACGAGATACGACTATAAGAGATCCGAG

Intron 2 GTATGGAGGG ... AATACGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.43	1.6	9	6
A2	100	0	4	2
A3	51.34	5.9	8	4
A4	52.94	4	6	3
A5	100	0	4	2
A6	68.44	9.8	27	14
A7	57.98	9.9	15	12
A8	60.95	7.1	13	9
A9	66.81	4.9	10	9
A10	51.21	11.5	17	5

>TRINITY_DN115620_c1_g2_i3 from sample A3 / Example spliced isoform

GGTATCAGTCAGAGCAAGCTTGATAAACCAATAATTATTTATTAAATAGTAAATACCGAGGAAAGGAAGCATAAGACGAAGAGCGAAGGCAAAGAGAACAGCAGGCACAAGGTGAGATATGGCGAACGCAACAGCAGCCTCAAATGCTCTGAGGAGAGGTCTCAACCTTCTGGCCACATCAAATGCTGCTCTCCGCCGTC
CAGTCGGCCACATTAGAGGCACTGTACTGTAATATTGCTTCAAATGGGTCATACATTCACAATAATCCACCCAGCTTCCGCCAACGATATTTATCGATCACATGGTCTATGTAAATATGGAAGAATGGCTAGGGTTTTTAGGGTTGCTATCTTCTCTTGAGGTTGTCTGGTCATTCTTTTTTATCATGCTAATTTCTATT
ATCTATGCTATTACGGACAAATCCTCTGAAAATAACTGGTGAAAGTTCTCCATGACGATGATCTGCTGTCTTCCTAGAGTTTATTATTCACTTAAACGAGATACGACTATAAGAGATTCGAGGGCCCCAAATTGCTAGAGGCCGAGGGGTGGCCAAGCGATCAGGGCCTGGTGCAAGTCCTATGTGACTTTGATGGAACA
GATACCCCCACCTGCTCCTTCTTAGCAGAAGTGATCTTCATTATTTCTTGTAACCTGCTGGTTACATTGCATTGTAATTTTCCAGATTGGAGAAAGGGAATATGAAACATCTTATCAAATAAAGGTGCAGAATGTAAATGCTCACTGCCACCGTGGTTTTTGTAGGATGATGGTCAGAAGAGGATTACACTTGGATTTCG
AGGAACTGTGGCACAGGGTTTTAAGCATCCTGTGGTAGTTTAGTTTTTCTTGCTTTGGCATTTTGAAATCCATTCCTGTGAAAATGCTTTATTTAGAAAGATCCATTCCTGTGAAAATGCTTTATTGAGAAAGATCCATTCCCGTGAAAATGTTTTATTTATAGAGATTGACGATGTCAATGTCAATTTCTTGTTCCAGG
ACTGTATTCCTGAAATAGCTTGCTTGGTAAGATTTCAAGTGTAAAACTTCAAAACATTGGTTTGGTCGTTGCTATATTCCAGATTATGGATCCAATAAATACGAGCTTTATAG

>TRINITY_DN115620_c1_g2_i6 from sample A3 / Example unspliced isoform:

GGTATCAGTCAGAGCAAGCTTGATAAACCAATAATTATTTATTAAATAGTAAATACCGAGGAAAGGAAGCATAAGACGAAGAGCGAAGGCAAAGAGAACAGCAGGCACAAGGTGAGATATGGCGAACGCAACAGCAGCCTCAAATGCTCTGAGGAGAGGTCTCAACCTTCTGGCCACATCAAATGCTGCTCTCCGCCGTC
CAGTCGGCCACATTAGAGGCACTGTACTGTAATATTGCTTCAAATGGGTCATACATTCACAATAATCCACCCAGCTTCCGCCAACGATATTTATCGATCACATGGTCTATGTAAATATGGAAGAATGGCTAGGGTTTTTAGGGTTGCTATCTTCTCTTGAGGTTGTCTGGTCATTCTTTTTTATCATGCTAATTTCTATT
ATCTATGCTATTACGGACAAATCCTCTGAAAATAACTGGTGAAAGTTCTCCATGACGATGATCTGCTGTCTTCCTAGAGTTTATTATTCACTTAAACGAGATACGACTATAAGAGATTCGAGGGCCCCAAATTGCTAGAGGCCGAGgtatggagggaggtatccctttatcgcttctctatctagggcactgggcacata
tcagatccgtttatagtatccatgtcgtttttttttcttctgtaagtacattaactaaatggtttataatacggcagGGGTGGCCAAGCGATCAGGGCCTGGTGCAAGTCCTATGTGACTTTGATGGAACAGATACCCCCACCTGCTCCTTCTTAGCAGAAGTGATCTTCATTATTTCTTGTAACCTGCTGGTTACATTG
CATTGTAATTTTCCAGATTGGAGAAAGGGAATATGAAACATCTTATCAAATAAAGGTGCAGAATGTAAATGCTCACTGCCACCGTGGTTTTTGTAGGATGATGGTCAGAAGAGGATTACACTTGGATTTCGAGGAACTGTGGCACAGGGTTTTAAGCATCCTGTGGTAGTTTAGTTTTTCTTGCTTTGGCATTTTGAAAT
CCATTCCTGTGAAAATGCTTTATTTAGAAAGATCCATTCCTGTGAAAATGCTTTATTGAGAAAGATCCATTCCCGTGAAAATGTTTTATTTATAGAGATTGACGATGTCAATGTCAATTTCTTGTTCCAGGACTGTATTCCTGAAATAGCTTGCTTGGTAAGATTTCAAGTGTAAAACTTCAAAACATTGGTTTGGTCGT
TGCTATATTCCAGATTATGGATCCAATAAATACGAGCTTTATAG

Intron 3 GTTGTCTGGT ... CTGGTGAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.61	2	10	9
A2	66.67	1	2	2
A3	79.48	4	16.7	14.3
A4	78.95	2	9	6
A5	80	2	7	9
A6	95.11	1	13	26
A7	79.83	5	17	22.7
A8	75.84	6	22	15.7
A9	76.19	5	13	19
A10	79.42	6	22	24.3

>TRINITY_DN38464_c0_g2_i7 from sample A9 / Example spliced isoform

CTAAAAATAAGCGTTGGAGAGGAAGGGAAAGGAATGATCCCCTACGTTGTTATGATGTGAAAATTTAAAGGAGTTTAATAAGAAGGTTAGACACCCCGAGTTCCCATCTAACAAAGCTGTTGACGGTATCAGTCAGAGCAAGCTTGATAAACCAATAATTATTTATTAAATAGTAAATACCGAGGAAAGGAAGCATAAGA
CGAAGAGCGAAGGCAAAGAGAACAGCAGGCACAAGGTGAGATATGGCGAACGCAACAGCAGCCTCAAATGCTCTGAGGAGAGGTCTCAACCTTCTGGCCACATCAAATGCTGCTCTCCGCCGTCCAGGTACTTTCCCCTCTCTCTCTATCTTTAAATCTTCTTTTCTGCTAGGGTTTGTTATTTAATTCACGAAGTTCCG
TTTCAATTCCTGCAGTCGGCCACATTAGAGGCACTGTACTGTAATATTGCTTCAAATGGGTCATACATTCACAATAATCCACCCAGCTTCCGCCAACGATATTTATCGATCACATGGTCTATGTAAATATGGAAGAATGGCTAGGGTTTTTAGGGTTGCTATCTTCTCTTGAGTTCTCCATGACGATGATCTGCTGTCTT
CCTAGAGTTTATTATTCACTTAAACGAGATACGACTATAAGAGATTCGAGGGCCCCAAATTGCTAGAGGCCGAGGGGTGGCCAAGCGATCAGGGCCTGGTGCAAGTCCTATGTGACTTTGATGGAACAGATACCCCCACCTGCTCCTTCTTAGCAGAAGTGATCTTCATTATTTCTTGTAACCTGCTGGTTACATTGCAT
TGTAATTTTCCAGATTGGAGAAAGGGAATATGAAACATCTTATCAAATAAAGGTGCAGAATGTAAATGCTCACTGCCACCGTGGTTTTTGTAGGATGATGGTCAGAAGAGGATTACACTTGGATTTCGAGGAACTGTGGCACAGGGTTTTAAGCATCCTGTGGTAGTTTAGTTTTTCTTGCTTTGGCATTTTGAAATCCA
TTCCTGTGAAAATGCTTTATTTAGAAAGATCCATTCCTGTGAAAATGCTTTATTTAGAAAGATCCATTCCTGTGAAAATGCTTT

>TRINITY_DN38464_c0_g2_i5 from sample A9 / Example unspliced isoform:

CTAAAAATAAGCGTTGGAGAGGAAGGGAAAGGAATGATCCCCTACGTTGTTATGATGTGAAAATTTAAAGGAGTTTAATAAGAAGGTTAGACACCCCGAGTTCCCATCTAACAAAGCTGTTGACGGTATCAGTCAGAGCAAGCTTGATAAACCAATAATTATTTATTAAATAGTAAATACCGAGGAAAGGAAGCATAAGA
CGAAGAGCGAAGGCAAAGAGAACAGCAGGCACAAGGTGAGATATGGCGAACGCAACAGCAGCCTCAAATGCTCTGAGGAGAGGTCTCAACCTTCTGGCCACATCAAATGCTGCTCTCCGCCGTCCAGTCGGCCACATTAGAGGCACTGTACTGTAATATTGCTTCAAATGGGTCATACATTCACAATAATCCACCCAGCT
TCCGCCAACGATATTTATCGATCACATGGTCTATGTAAATATGGAAGAATGGCTAGGGTTTTTAGGGTTGCTATCTTCTCTTGAGgttgtctggtcattcttttttatcatgctaatttctattatctatgctattacggacaaatcctctgaaaataactggtgaaagTTCTCCATGACGATGATCTGCTGTCTTCCTA
GAGTTTATTATTCACTTAAACGAGATACGACTATAAGAGATTCGAGGGCCCCAAATTGCTAGAGGCCGAGGTATGGAGGGAGGTATCCCTTTATCGCTTCTCTATCTAGGGCACTGGGCACATATCAGATCCGTTTATAGTATCCATGTCGTTTTTTTTTCTTCTGTAAGTACATTAACTAAATGGTTTATAATACGGCA
GGGGTGGCCAAGCGATCAGGGCCTGGTGCAAGTCCTATGTGACTTTGATGGAACAGATACCCCCACCTGCTCCTTCTTAGCAGAAGTGATCTTCATTATTTCTTGTAACCTGCTGGTTACATTGCATTGTAATTTTCCAGATTGGAGAAAGGGAATATGAAACATCTTATCAAATAAAGGTGCAGAATGTAAATGCTCAC
TGCCACCGTGGTTTTTGTAGGATGATGGTCAGAAGAGGATTACACTTGGATTTCGAGGAACTGTGGCACAGGGTTTTAAGCATCCTGTGGTAGTTTAGTTTTTCTTGCTTTGGCATTTTGAAATCCATTCCTGTGAAAATGCTTTATTTAGAAAGATCCATTCCTGTGAAAATGCTTTATTTAGAAAGATCCATTCCTGT
GAAAATGCTTT