sp|F4JNX3|CMKMT_ARATH ----> Calmodulin-lysine N-methyltransferase

Intron 1 GTAAGTGTGG ... TATCACACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.83	41.5	4.2	16
A2	19.97	48.2	4.8	12
A3	19.35	46.1	7.5	11
A4	14.71	40.6	2.2	7
A5	23.9	15.9	1.5	5
A6	30.9	118.7	9.2	53
A7	30.67	205.8	18.5	91
A8	26.13	156.1	20.5	55
A9	28.17	214.5	20.5	84
A10	27.13	191.4	28.5	71

>TRINITY_DN119378_c0_g1_i5 from sample A1 / Example spliced isoform

CACCTATATGCCGCTTTGATGACATTAATCTTCTTGGAATGCCACTAGAGGGACCCGTTTAGCATATGCTTCTATTATCAGTACTCTCAAGAGCAACGAATTTACCCTTCAACCACAGGTCATTCGGTTGCGAATAGCGCAACTGAATCGAATGAAATTCAGGGGACTGTGAAGGGAAACATGGACACTGCACTCTTCTC
TCCATTCTCTCTTTTCAGCACTGAAGATAAACTCTCTGAGTCAGATTCAGAAGATACCCAAACATTCGAGGAAAGAGAGCATACATTTCCAGGAATGGTTTTGCTTATTCGAGAATTTTCATTTCATCAAGTTAATGCAAACATGCTTTGGCCAGGGGCATCTATCTTTGCAGAGTGGTTGATTGAACACCAAAACCTCC
TCAAAGGTCGTCAAATTATAGAGCTGGGAAGTGGTACAGGAGCGTTGGCTATTTTTCTGAAGAAATCTCTTGCTGTTGATATCACTACTTCAGATTTTGATGACAAAGAGATTGAAGAGAATATTGCATATAACTGTACAGCTAATGGAATGTCAGTCTTGCCACACATCCGACACACGTGGGGTCAAGAGTTTGGCTTG
CAGCATTCAGACTGGGATTTGATTCTTGCAAGTGATATTTTACTCTATGTGAAGCAGTATCCAAATTTGATCCAGACACTTTTATTTCTCCTTAAAGCATATAGACCTGTGGATAAGAATCCAGATATTACAGGTGATAAATGCAGCCAAGTGAGGGCAATGGACCAGCCAATGGAGGCAGAAGTAAAAGAGAACTACCA
TGATAAAAAAAGTCTGCAACAACATAGTGAGAGTTTTCAAAGAAAGGAGGCAAATGGGGAAAAAGATGAACTGCCAAGACCATGCTTCTTGATGAGTTGGCGACGCCGGATCCCAAAGGATGATGAAGCATCCTTTTTCAATGGCTGTGAGAAGGCAGGACTGATGGTGCAACATATGGGATCTCGGGTTTTTTGCATCA
GTATTGGAAAATCAATTGATGTCTAGTTTTATATGTATGTTAATCTGCATGCTTGATAGAACTAAGCATTCAAAATCCTCTTAGCTAGCTTGTGCGACTGAAATATTATTCCCTAAGCATTCTAAATTTCCTTGGCCTTACAAAATACTTTTTGCCACAATATTGTATGGTTAATCTGTTTACAGAAAGTAGTTTTTAAG
ATGGTGCATTACATCAGATGGGTAAGTACATAAATATATTTCTAGGGCAGACTTCCTTCGACTAAGGTGCCAATTTTGAGAGGTACCTATTCTGCCAAACTCATCTAGGAATTGCACCTAGTCTCATCCTTTTGAAACTTATGCCATTTGGGTGGGGCAGACCACAACACGAATGGAGCCCTGGTGGGCAGCTCAACACT
CCGTTGACCTTGTCAGTGAATCAATGCCCCATGGACAATTTGTGAGATGCCTTTGACAAAAGTCTAAAATGAGAGCTCATGCTTTTGTCATTGATATCTAGTCTGCAGATAACGGGTAGCTGCTTTTTGCTAAGATTGTAGCCTTTCAGTGAGAGCACAAGCATGGAAATGTTGCAGCCTTCTAGGCAAAACTCAGGTGG
GCTTTTGAGAGCCCAGTAGAGGCTGTTTCCATCTTGGTGGCAGCCTGCGATTTAACATAACTACTACTGCCCATTTTTTTCGCTTCTCAACATCTTAGGGTTACCTGTTTAAATTACTGCCCAACATTGTGGCTCAATGACTGC

>TRINITY_DN119378_c0_g1_i10 from sample A1 / Example unspliced isoform:

CACCTATATGCCGCTTTGATGACATTAATCTTCTTGGAATGCCACTAGAGGGACCCGTTTAGCATATGCTTCTATTATCAGTACTCTCAAGAGCAACGAATTTACCCTTCAACCACAGGTCATTCGGTTGCGAATAGCGCAACTGAATCGAATGAAATTCAGGGGACTGTGAAGGGAAACATGGACACTGCACTCTTCTC
TCCATTCTCTCTTTTCAGCACTGAAGATAAACTCTCTGAGTCAGATTCAGAAGATACCCAAACATTCGAGGAAAGAGAGCATACATTTCCAGGAATGGGGCATCTATCTTTGCAGAGTGGTTGATTGAACACCAAAACCTCCTCAAAGGTCGTCAAATTATAGAGCTGGGAAGTGGTACAGGAGCGTTGGCTATTTTTCT
GAAGAAATCTCTTGCTGTTGATATCACTACTTCAGATTTTGATGACAAAGAGATTGAAGAGAATATTGCATATAACTGTACAGCTAATGGAATGTCAGTCTTGCCACACATCCGACACACGTGGGGTCAAGAGTTTGGCTTGCAGCATTCAGACTGGGATTTGATTCTTGCAAGTGATATTTTACTCTgtaagtgtggga
attgctcagaaacgtgatgaactctttcttgttgtagataaacatgcaattagtgtcctatttccactggtttcataagctggtaatttgttggacgaggactgaacaatctgtttttatgcaatatcacacagATGTGAAGCAGTATCCAAATTTGATCCAGACACTTTTATTTCTCCTTAAAGCATATAGACCTGTGG
ATAAGAATCCAGATATTACAGGTGATAAATGCAGCCAAGTGAGGGCAATGGACCAGCCAATGGAGGCAGAAGTAAAAGAGAACTACCATGATAAAAAAAGTCTGCAACAACATAGTGAGAGTTTTCAAAGAAAGAAGGC

Intron 2 GTTTTGCTTA ... AGCTGGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.67	27.3	33	35
A2	47.66	57	49.4	48
A3	51.53	67	69.4	58.2
A4	51.65	53.9	56	38.2
A5	64.53	16.2	29	16
A6	55.63	118.4	146.3	69.7
A7	50.13	208.2	203.9	123.1
A8	53.77	140.8	160	93
A9	51.4	219.4	225.9	140.7
A10	46.06	218.5	181.4	123

>TRINITY_DN119378_c0_g1_i2 from sample A1 / Example spliced isoform

GCCACAGGTCATTCGGTTGCGAGGGGAAACATGGACACTGCACTCTTCTCTCCATTCTCTCTTTTCAGCACTGAAGATAAACTCTCTGAGTCAGATTCAGAAGATACCCAAACATTCGAGGAAAGAGAGCATACATTTCCAGGAATGTGGTACAGGAGCGTTGGCTATTTTTCTGAAGAAATCTCTTGCTGTTGATATCA
CTACTTCAGATTTTGATGACAAAGAGATTGAAGAGAATATTGCATATAACTGTACAGCTAATGGAATGTCAGTCTTGCCACACATCCGACACACGTGGGGTCAAGAGTTTGGCTTGCAGCATTCAGACTGGGATTTGATTCTTGCAAGTGATATTTTACTCTATGTGAAGCAGTATCCAAATTTGATCCAGACACTTTTA
TTTCTCCTTAAAGCATATAGACCTGTGGATAAGAATCCAGATATTACAGGTGATAAATGCAGCCAAGTGAGGGCAATGGACCAGCCAATGGAGGCAGAAGTAAAAGAGAACTACCATGATAAAAAAAGTCTGCAACAACATAGTGAGAGTTTTCAAAGAAAGGAGGCAAATGGGGAAAAAGATGAACTGCCAAGACCATG
CTTCTTGATGAGTTGGCGACGCCGGATCCCAAAGGATGATGAAGCATCCTTTTTCAATGGCTGTGAGAAGGCAGGACTGATGGTGCAACATATGGGATCTCGGGTTTTTTGCATCAGTATTGGAAAAACAGTCTTAGTTGATTTTATGGTTTTAAAGGACCCTATGGATTTTGATATGATCCTCAGGCGTGT

>TRINITY_DN119378_c0_g1_i12 from sample A1 / Example unspliced isoform:

GCCACAGGTCATTCGGTTGCGAGGGGAAACATGGACACTGCACTCTTCTCTCCATTCTCTCTTTTCAGCACTGAAGATAAACTCTCTGAGTCAGATTCAGAAGATACCCAAACATTCGAGGAAAGAGAGCATACATTTCCAGGAATGgttttgcttattcgagaattttcatttcatcaagttaatgcaaacatgctttg
gccaggggcatctatctttgcagagtggttgattgaacaccaaaacctcctcaaaggtcgtcaaattatagagctgggaagTGGTACAGGAGCGTTGGCTATTTTTCTGAAGAAATCTCTTGCTGTTGATATCACTACTTCAGATTTTGATGACAAAGAGATTGAAGAGAATATTGCATATAACTGTACAGCTAATGGAA
TGTCAGTCTTGCCACACATCCGACACACGTGGGGTCAAGAGTTTGGCTTGCAGCATTCAGACTGGGATTTGATTCTTGCAAGTGATATTTTACTCTATGTGAAGCAGTATCCAAATTTGATCCAGACACTTTTATTTCTCCTTAAAGCATATAGACCTGTGGATAAGAATCCAGATATTACAGGTGATAAATGCAGCCAA
GTGAGGGCAATGGACCAGCCAATGGAGGCAGAAGTAAAAGAGAACTACCATGATAAAAAAAGTCTGCAACAGCATAGTGAGAGTTTTCAAAGAAAGGAGGCAAATGGGGAAAAAGATGAACTGCCAAGACCATGCTTCTTGATGAGTTGGCGACGCCGGATCCCAAAGGATGATGAAGCATCCTTTTTCAATGGCTGTGA
GAAGGCAGGACTGATGGTGCAACATATGGGATCTCGGGTTTTTTGCATCAGTATTGGAAAAACAGTCTTAGTTGATTTTATGGTTTTAAAGGACCCTATGGATTTTGATATGATCCTCAGGCGTGT

Intron 3 GTTTTGCTTA ... ATCTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76.74	10	33	33
A2	92.77	3	49	28
A3	89.74	6	69	36
A4	91.21	4	56	27
A5	95.12	1	29	10
A6	92.27	8	145	46
A7	91.16	13	203	65
A8	90.73	12	160	75
A9	93	12	225	94
A10	85.16	21	181	60

>TRINITY_DN119378_c0_g1_i4 from sample A1 / Example spliced isoform

AGAGCATACATTTCCAGGAATGAGTGGTTGATTGAACACCAAAACCTCCTCAAAGGTCGTCAAATTATAGAGCTGGGAAGTGGTACAGGAGCGTTGGCTATTTTTCTGAAGAAATCTCTTGCTGTTGATATCACTACTTCAGATTTTGATGACAAAGAGATTGAAGAGAATATTGCATATAACTGTACAGCTAATGGAAT
GTCAGTCTTGCCACACATCCGACACACGTGGGGTCAAGAGTTTGGCTTGCAGCATTCAGACTGGGATTTGATTCTTGCAAGTGATATTTTACTCTATGTGAAGCAGTATCCAAATTTGATCCAGACACTTTTATTTCTCCTTAAAGCATATAGACCTGTGGATAAGAATCCAGATATTACAGGTGATAAATGCAGCCAAG
TGAGGGCAATGGACCAGCCAATGGAGGCAGAAGTAAAAGAGAACTACCATGATAAAAAAAGTCTGCAACAACATAGTGAGAGTTTTCAAAGAAAGGAGGCAAATGGGGAAAAAGATGAACTGCCAAGACCATGCTTCTTGATGAGTTGGCGACGCCGGATCCCAAAGGATGATGAAGCATCCTTTTTCAATGGCTGTGAG
AAGGCAGGACTGATGGTGCAACATATGGGATCTCGGGTTTTTTGCATCAGTATTGGAAAATCAATTGATGTCTAGTTTTATATGTATGTTAATCTGCATGCTTGATAGAACTAAGCATTCAAAATCCTCTTAGCTAGCTTGTGCGACTGAAATATTATTCCCTAAGCATTCTAAATTTCCTTGGCCTTACAAAATACTTT
TTGCCACAATATTGTATGGTTAATCTGTTTACAGAAAGTAGTTTTTAAGATGGTGCATTACACCAGATGGGTAAGGACATAAATATATTTCTAGGGCAGACTTCCTTCGACTAAGGTGCCAATTTTGAGAGGTACCTATTCTGCCAAACTCATCTAGGAATTGCACCTAGTCTCATCCTTTTGAAACTTATGCCATTTGG
GTGGGGCAGACCACAACACGAATGGAGCCCTGGTGGGCAGCTCAACACTCCGTTGACCTTGTCAGTGAATCAATGCCCCATGGACAATTTGTGAGATGCCTTTGACAAAAGTCTAAAATGAGAGCTCATGCTTTTGTCATTGATATCTAGTCTGCAGATAACGGGTAGCTGCTTTTTGCTAAGATTGTAGCCTTTCAGTG
AGAGCACAAGCATGGAAATGTTGCAGCCTTCTAGGCAAAACTCAGGTGGGCTTTTGAGAGCCCAGTAGAGGCTGTTTCCATCTTGGTGGCAGCCTGCGATTTAACATAACTACTACTGCCCATTTTTTTCGCTTCTCAACATCTTAGGGTTACCTGTTTAAATTACTGCCCAACATTGTGGCTCAATGACTGC

>TRINITY_DN119378_c0_g1_i8 from sample A1 / Example unspliced isoform:

CACCTATATGCCGCTTTGATGACATTAATCTTCTTGGAATGCCACTAGAGGGACCCGTTTAGCATATGCTTCTATTATCAGTACTCTCAAGAGCAACGAATTTACCCTTCAGCCACAGGTCATTCGGTTGCGAGGGGAAACATGGACACTGCACTCTTCTCTCCATTCTCTCTTTTCAGCACTGAAGATAAACTCTCTGA
GTCAGATTCAGAAGATACCCAAACATTCGAGGAAAGAGAGCATACATTTCCAGGAATGgttttgcttattcgagaattttcatttcatcaagttaatgcaaacatgctttggccaggggcatctatctttgcagAGTGGTTGATTGAACACCAAAACCTCCTCAAAGGTCGTCAAATTATAGAGCTGGGAAGTGGTACAG
GAGCGTTGGCTATTTTTCTGAAGAAATCTCTTGCTGTTGATATCACTACTTCAGATTTTGATGACAAAGAGATTGAAGAGAATATTGCATATAACTGTACAGCTAATGGAATGTCAGTCTTGCCACACATCCGACACACGTGGGGTCAAGAGTTTGGCTTGCAGCATTCAGACTGGGATTTGATTCTTGCAAGTGATATT
TTACTCTATGTGAAGCAGTATCCAAATTTGATCCAGACACTTTTATTTCTCCTTAAAGCATATAGACCTGTGGATAAGAATCCAGATATTACAGGTGATAAATGCAGCCAAGTGAGGGCAATGGACCAGCCAATGGAGGCAGAAGTAAAAGAGAACTACCATGATAAAAAAAGTCTGCAACAACATAGTGAGAGTTTTCA
AAGAAAGGAGGCAAATGGGGAAAAAGATGAACTGCCAAGACCATGCTTCTTGATGAGTTGGCGACGCCGGATCCCAAAGGATGATGAAGCATCCTTTTTCAATGGCTGTGAGAAGGCAGGACTGATGGTGCAACATATGGGATCTCGGGTTTTTTGCATCAGTATTGGAAAATCAATTGATGTCTAGTTTTATATGTATG
TTAATCTGCATGCTTGATAGAACTAAGCATTCAAAATCCTCTTAGCTAGCTTGTGCGACTGAAATATTATTCCCTAAGCATTCTAAATTTCCTTGGCCTTACAAAATACTTTTTGCCACAATATTGTATGGTTAATCTGTTTACAGAAAGTAGTTTTTAAGATGGTGCATTACATCAGATGGGTAAGTACATAAATATAT
TTCTAGGGCAGACTTCCTTCGACTAAGGTGCCAATTTTGAGAGGTACCTATTCTGCCAAACTCATCTAGGAATTGCACCTAGTCTCATCCTTTTGAAACTTATGCCATTTGGGTGGGGCAGACCACAACACGAATGGAGCCCTGGTGGGCAGCTCAACACTCCGTTGACCTTGTCAGTGAATCAATGCCCCATGGACAAT
TTGTGAGATGCCTTTGACAAAAGTCTAAAATGAGAGCTCATGCTTTTGTCATTGATATCTAGTCTGCAGATAACGGGTAGCTGCTTTTTGCTAAGATTGTAGCCTTTCAGTGAGAGCACAAGCATGGAAATGTTGCAGCCTTCTAGGCAAAACTCAGGTGGGCTTTTGAGAGCCCAGTAGAGGCTGTTTCCATCTTGGTG
GCAGCCTGCGATTTAACATAACTACTACTGCCCATTTTTTTCGCTTCTCAACATCTTAGGGTTACCTGTTTAAATTACTGCCCAACATTGTGGCTCAATGACTGC

Intron 4 GTTTTGCTTA ... CTTTGGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60.15	21.1	33	29.8
A2	65.65	20.7	49.3	29.8
A3	54.05	44.6	69.3	35.5
A4	54.4	33.8	56	24.5
A5	59.97	13.7	29	12
A6	52.64	88.7	145.9	51
A7	49.25	138	203.6	64
A8	51.32	115.3	160	82.8
A9	50.32	158.4	225.6	95
A10	45.06	145.7	181.3	57.5

>TRINITY_DN119378_c0_g1_i10 from sample A1 / Example spliced isoform

CACCTATATGCCGCTTTGATGACATTAATCTTCTTGGAATGCCACTAGAGGGACCCGTTTAGCATATGCTTCTATTATCAGTACTCTCAAGAGCAACGAATTTACCCTTCAACCACAGGTCATTCGGTTGCGAATAGCGCAACTGAATCGAATGAAATTCAGGGGACTGTGAAGGGAAACATGGACACTGCACTCTTCTC
TCCATTCTCTCTTTTCAGCACTGAAGATAAACTCTCTGAGTCAGATTCAGAAGATACCCAAACATTCGAGGAAAGAGAGCATACATTTCCAGGAATGGGGCATCTATCTTTGCAGAGTGGTTGATTGAACACCAAAACCTCCTCAAAGGTCGTCAAATTATAGAGCTGGGAAGTGGTACAGGAGCGTTGGCTATTTTTCT
GAAGAAATCTCTTGCTGTTGATATCACTACTTCAGATTTTGATGACAAAGAGATTGAAGAGAATATTGCATATAACTGTACAGCTAATGGAATGTCAGTCTTGCCACACATCCGACACACGTGGGGTCAAGAGTTTGGCTTGCAGCATTCAGACTGGGATTTGATTCTTGCAAGTGATATTTTACTCTGTAAGTGTGGGA
ATTGCTCAGAAACGTGATGAACTCTTTCTTGTTGTAGATAAACATGCAATTAGTGTCCTATTTCCACTGGTTTCATAAGCTGGTAATTTGTTGGACGAGGACTGAACAATCTGTTTTTATGCAATATCACACAGATGTGAAGCAGTATCCAAATTTGATCCAGACACTTTTATTTCTCCTTAAAGCATATAGACCTGTGG
ATAAGAATCCAGATATTACAGGTGATAAATGCAGCCAAGTGAGGGCAATGGACCAGCCAATGGAGGCAGAAGTAAAAGAGAACTACCATGATAAAAAAAGTCTGCAACAACATAGTGAGAGTTTTCAAAGAAAGAAGGC

>TRINITY_DN119378_c0_g1_i12 from sample A1 / Example unspliced isoform:

GCCACAGGTCATTCGGTTGCGAGGGGAAACATGGACACTGCACTCTTCTCTCCATTCTCTCTTTTCAGCACTGAAGATAAACTCTCTGAGTCAGATTCAGAAGATACCCAAACATTCGAGGAAAGAGAGCATACATTTCCAGGAATGgttttgcttattcgagaattttcatttcatcaagttaatgcaaacatgctttg
gccagGGGCATCTATCTTTGCAGAGTGGTTGATTGAACACCAAAACCTCCTCAAAGGTCGTCAAATTATAGAGCTGGGAAGTGGTACAGGAGCGTTGGCTATTTTTCTGAAGAAATCTCTTGCTGTTGATATCACTACTTCAGATTTTGATGACAAAGAGATTGAAGAGAATATTGCATATAACTGTACAGCTAATGGAA
TGTCAGTCTTGCCACACATCCGACACACGTGGGGTCAAGAGTTTGGCTTGCAGCATTCAGACTGGGATTTGATTCTTGCAAGTGATATTTTACTCTATGTGAAGCAGTATCCAAATTTGATCCAGACACTTTTATTTCTCCTTAAAGCATATAGACCTGTGGATAAGAATCCAGATATTACAGGTGATAAATGCAGCCAA
GTGAGGGCAATGGACCAGCCAATGGAGGCAGAAGTAAAAGAGAACTACCATGATAAAAAAAGTCTGCAACAGCATAGTGAGAGTTTTCAAAGAAAGGAGGCAAATGGGGAAAAAGATGAACTGCCAAGACCATGCTTCTTGATGAGTTGGCGACGCCGGATCCCAAAGGATGATGAAGCATCCTTTTTCAATGGCTGTGA
GAAGGCAGGACTGATGGTGCAACATATGGGATCTCGGGTTTTTTGCATCAGTATTGGAAAAACAGTCTTAGTTGATTTTATGGTTTTAAAGGACCCTATGGATTTTGATATGATCCTCAGGCGTGT