NA ----> NA

Intron 1 GTAGCTAGAT ... ATGCTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.86	14	22.3	42.4
A2	40.96	47.9	14	52.5
A3	38.24	37	10	35.8
A4	41.34	33.9	17	30.8
A5	42.22	26	10	28
A6	30.18	175.7	36.6	115.2
A7	35.93	183.1	60.6	144.5
A8	40.32	195.9	86.3	177.8
A9	26.11	218.4	30	124.2
A10	30.6	137	31.3	89.2

>TRINITY_DN127125_c3_g1_i1 from sample A1 / Example spliced isoform

GTTAGGTTAAAGAGAAAATCTAGTGGATGGCATCTTAATAGTGAACGCGATGGAGCCAAACAATGAACGATACTCACATGAGATAACGTTATGGGAAATACTGAAAGATACACAGGCACAAATTGAATTCATGGGGAACATTCTGAATCCGGTGTAACAGTTGTAGTTTCTGTGATCGTAGTTCTTATTTCGTTGCTGAG
CCATGTCGAGATCTATTGCAGCTTATAGTTCCTACTTTTCTGTTGAGATTCGCAGTCTTCTACCATGTAGCCATGTAGTCGGAGCTGCGAGAGTATGGTTGAAGTACGACGCCCGAACCACGAATCGCTTATTGCACTGCTAACATAATAGAGAATAGATTTGGAATCACTTACTGCACTGCTAACGCAATAGAGAATAG
ATTTGGAATGTAGAAAATCATTTTTTCAGATTAGCTACTTCTTCTTTGTCTACACGATATTGCAACTAAGCGCTAGCTTCTCCAGCAAGGAGCCTCTGGTTGGACAAATTCCATCCCCTTCCATGAATATATTGCACGAGAAACTGAAGGTAACCTTGTGTTGGATATATCATAATTTATGAGCTGATCGCGTATTCACT
GCGTCGGCTCTGTCAATCACCCTGTTAGCCGTTGTTCGTGTCAATGCGATGTTTGTGGCATCAGTCTGTAACCACGTTAATAGTTCGCAAATCAAGAAAACATA

>TRINITY_DN127125_c3_g1_i4 from sample A1 / Example unspliced isoform:

GTTAGGTTAAAGAGAAAATCTAGTGGATGGCATCTTAATAGTGAACGCGATGGAGCCAAACAATGAACGATACTCACATGAGATAACgtagctagattttattgtttttcttgcattgtaggtatctagatcttagggtttttcttgcatttcttgctaactatgatgtttttatgctttcagGTTATGGGAAATACTGA
AAGATACACAGGCACAAATTGAATTCATGGGGAACATTCTGAATCCGGTGTAACAGTTGTAGTTTCTGTGATCGTAGTTCTTATTTCGTTGCTGAGCCATGTCGAGATCTATTGCAGCTTATAGTTCCTACTTTTCTGTTGAGATTCGCAGTCGTGAGTTCTATCCTATAGCTTCCTCTCTAAATTGATAACAAACATAG
TACTTTCATGCTAACTGTGATGTTTTTATGCTTTCAAGTTCTACCATGTAGCCATGTAGTCGGAGCTGCGAGAGTATGGTTGAAGTACGACGCCCGAACCACGAATCGCTTATTGCACTGCTAACATAATAGAGAATAGATTTGGAATCACTTACTGCACTGCTAACGCAATAGAGAATAGATTTGGAATGTAGAAAATC
ATTTTTTCAGATTAGCTACTTCTTCTTTGTCTACACGATATTGCAACTAAGCGCTAGCTTCTCCAGCAAGGAGCCTCTGGTTGGACAAATTCCATCCCCTTCCATGAATATATTGCACGAGAAACTGAAGGTAACCTTGTGTTGGATATATCATAATTTATGAGCTGATCGCGTATTCACTGCGTCGGCTCTGTCAATCA
CCCTGTTAGCCGTTGTTCGTGTCAATGCGATGTTTGTGGCATCAGTCTGTAACCACGTTAATAGTTCGCAAATCAAGAAAACATA

Intron 2 GTGAGTTCTA ... TGCTTTCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	57.3	34.2	33	58.5
A2	64.58	17	31	31
A3	63.79	18.5	35	30
A4	57.79	17.5	19	28.8
A5	51.48	9.9	6	15
A6	45.54	111	87	98.6
A7	55.97	118.2	139	161.5
A8	42.4	164.8	110	132.5
A9	43.7	170.8	124	141.2
A10	43.98	109.2	79	92.5

>TRINITY_DN127125_c3_g1_i3 from sample A1 / Example spliced isoform

GTTAGGTTAAAGAGAAAATCTAGTGGATGGCATCTTAATAGTGAACGCGATGGAGCCAAACAATGAACGATACTCACATGAGATAACGTAGCTAGATTTTAGTGTTTTTCTTGCATTGCAGGAATCTATATCTTAGGGTTTTTCTTGCATTTCTTGCTAACTATGATGTTTTTATGCTTTCAGGTTATGGGAAATACTGA
AAGATACACAGGCACAAATTGAATTCATGGGGAACATTCTGAATCCGGTGTAACAGTTGTAGTTTCTGTGATCGTAGTTCTTATTTCGTTGCTGAGCCATGTCGAGATCTATTGCAGCTTATAGTTCCTACTTTTCTGTTGAGATTCGCAGTCTTCTACCATGTAGCCATGTAGTCGGAGCTGCGAGAGTATGGTTGAAG
TACGACGCCCGAACCACGAATCGCTTATTGCACTGCTAACATAATAGAGAATAGATTTGGAATCACTTACTGCACTGCTAACGCAATAGAGAATAGATTTGGAATGTAGAAAATCATTTTTTCAGATTAGCTACTTCTTCTTTGTCTACACGATATTGCAACTAAGCGCTAGCTTCTCCAGCAAGGAGCCTCTGGTTGGA
CAAATTCCATCCCCTTCCATGAATATATTGCACGAGAAACTGAAGGTAACCTTGTGTTGGATATATCATAATTTATGAGCTGATCGCGTATTCACTGCGTCGGCTCTGTCAATCACCCTGTTAGCCGTTGTTCGTGTCAATGCGATGTTTGTGGCATCAGTCTGTAACCACGTTAATAGTTCGCAAATCAAGAAAACATA

>TRINITY_DN127125_c3_g1_i4 from sample A1 / Example unspliced isoform:

GTTAGGTTAAAGAGAAAATCTAGTGGATGGCATCTTAATAGTGAACGCGATGGAGCCAAACAATGAACGATACTCACATGAGATAACGTAGCTAGATTTTATTGTTTTTCTTGCATTGTAGGTATCTAGATCTTAGGGTTTTTCTTGCATTTCTTGCTAACTATGATGTTTTTATGCTTTCAGGTTATGGGAAATACTGA
AAGATACACAGGCACAAATTGAATTCATGGGGAACATTCTGAATCCGGTGTAACAGTTGTAGTTTCTGTGATCGTAGTTCTTATTTCGTTGCTGAGCCATGTCGAGATCTATTGCAGCTTATAGTTCCTACTTTTCTGTTGAGATTCGCAGTCgtgagttctatcctatagcttcctctctaaattgataacaaacatag
tactttcatgctaactgtgatgtttttatgctttcaagTTCTACCATGTAGCCATGTAGTCGGAGCTGCGAGAGTATGGTTGAAGTACGACGCCCGAACCACGAATCGCTTATTGCACTGCTAACATAATAGAGAATAGATTTGGAATCACTTACTGCACTGCTAACGCAATAGAGAATAGATTTGGAATGTAGAAAATC
ATTTTTTCAGATTAGCTACTTCTTCTTTGTCTACACGATATTGCAACTAAGCGCTAGCTTCTCCAGCAAGGAGCCTCTGGTTGGACAAATTCCATCCCCTTCCATGAATATATTGCACGAGAAACTGAAGGTAACCTTGTGTTGGATATATCATAATTTATGAGCTGATCGCGTATTCACTGCGTCGGCTCTGTCAATCA
CCCTGTTAGCCGTTGTTCGTGTCAATGCGATGTTTGTGGCATCAGTCTGTAACCACGTTAATAGTTCGCAAATCAAGAAAACATA