sp|O81416|GUN17_ARATH ----> Endoglucanase 17

Intron 1 ATAACGCCCT ... AGGATCTCAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.5	44	116	60.5
A2	59.73	91	135	38
A3	69.85	60	139	28
A4	83.51	16	81	36.5
A5	61.26	43	68	16.5
A6	60.75	460	712	192
A7	63.73	725	1274	356
A8	64.92	601	1112	326
A9	63.14	474	812	245.5
A10	65.35	519	979	255.5

>TRINITY_DN47904_c0_g1_i12 from sample A10 / Example spliced isoform

GGTTAACTAATGACTTAATCATTAGCTGCGTTCTTTCTGGAGGAGGCGTTGTGCGCTCGATGCTTTGTGAGCTGGAGGCGGCGTCTTTCTGGAGGCGGCGTTGTGCGCTCGATGCGTTGTGCGTTGGAGGCTATGCGTCGGGTTAACAAGATGCAGTTATGGCGGCGTTCTTCCTGTCATCGCCGTTGTATGCCGTCTGG
AGAGTTAGTTACGCATGTGTGAGTTAGATAAATACGTGGTGACTAACGGTTCAGAGAGAACGACAGCTTCTTCCCGATGAAGAGACCTCAGCCTGGTGTTTTTATCATTTCTATTCGCTGAGGGTTTTCGAGAGGTCCAGGCCATGCTGAAGGAGGTCGCCAAAGGTTTTCAAGATGTCGAGGCCATGCTGAAGGAGGTC
GCCGAGGGTTTTCAAGATGTCCAGGCTATGCTGAAGGAGGTCGCCGAGGGTTTTCAGGTCACCGAGGGTTTTCAAGATGTCCAGGCCATGCTGGAGGAGGATTTGTTGCAAGAGCATTCGCCTAGATCGATGGGATTGAGAGAGTCAAGGCGAATCACGATGCAGAGCAGAGCGCGGTTATCCCTGACATGCGAGGAGGC
GAGAACATTATCACATCAAGATTATAAACCCCTTGATGAACACAAGGGCCACGCCGACAACTTTATTTGCTCTCTCTTGTCGGGAACACCATCTTCGCAGGCGCAGTGCACATTTACACTAATTTATTTTATTTTAGTTTTGCAGGTGGACTGCTCTTCCAAATGAGTGATAGCAATTTGTAATATGTGACGTCCACCAC
TTTCCTGCTTTTCACTTATGCAAAATACTTAGGCGTTTCCAAGCAAGTGTTGAAATGCGGGAACATCATAGCCACTCCAACCCGAATACGTACTCTTGCAAAAAGACAATCACTGAGGAGTGACCGTTATATAAATATGGATTTGAGGTGCAAAGGGTGCTGTTAGGCTTGCTGCTGATGATCATTTTATAAATTGTATT
TCTTAACACGTGGACTACATACTAGGAGACAACCCACTGCGAATGTCAAACATGGTGGTTTACGGTGCCAAGTTTCCACAGCATATACATCACAGGGGCTCGTCGTTTCCTTCCATTTACAACCACCTCCAAACCATTCCCTGCAACGATGGACTTACATCTGGTAGCATGGCACATTTGTTGTTTTAAATGTTTTAAAA
CTATTGGCCTTATAATTAATGCTTCAACGTTCAAAGTGACTGTTGCACGTCTCTATGGCACATGGCACAAACCCATCCATTTGGCAAAGGAAAGAGTCTAGACTCTAAACCTTTAATCAATTCAAAGAAGAGTTCAATCTTTTAACCCATTTTAAGTATTCTTTCATTAAGTGAAAGAATATGATGCGAAAACTATAATG
TAT

>TRINITY_DN47904_c0_g1_i5 from sample A10 / Example unspliced isoform:

GGTTAACTAATGACTTAATCATTAGCTGCGTTCTTTCTGGAGGAGGCGTTGTGCGCTCGATGCTTTGTGAGCTGGAGGCGGCGTCTTTCTGGAGGCGGCGTTGTGCGCTCGATGCGTTGTGCGTTGGAGGCTATGCGTCGGGTTAACAAGATGCAGTTATGGCGGCGTTCTTCCTGTCATCGCCGTTGTATGCCGTCTGG
AGAGTTAGTTACGCATGTGTGAGTTAGATAAATACGTGGTGACTAACGGTTCAGAGAGAACGACAGCTTCTTCCCGATGAAGAGACCTCAGCCTGGTGTTTTTATCATTTCTATTCGCTGAGGGTTTTCGAGAGGTCCAGGCCATGCTGAAGGAGGTCGCCAAAGGTTTTCAAGATGTCGAGGCCATGCTGAAGGAGGTC
GCCGAGGGTTTTCAAGATGTCCAGGCTATGCTGAAGGAGGTCGCCGAGGGTTTTCAGGTCACCGAGGGTTTTCAAGATGTCCAGGCCATGCTGGAGGAGGATTTGTTGCAAGAGCATTCGCCTAGATCGATGGGATTGAGAGAGataacgccctcttactgtgtcctgcgcggatattattcagatcttctctttttgtt
gatattggtttgatgtttgaatgttaaaatggataaagtcatatgcaggatctcacTCAAGGCGAATCACGATGCAGAGCAGAGCGCGGTTATCCCTGACATGCGAGGAGGCGAGAACATTATCACATCAAGATTATAAACCCCTTGATGAACACAAGGGCCACGCCGACAACTTTATTTGCTCTCTCTTGTCGGGAACA
CCATCTTCGCAGGCGCAGTGCACATTTACACTAATTTATTTTATTTTAGTTTTGCAGGTGGACTGCTCTTCCAAATGAGTGATAGCAATTTGTAATATGTGACGTCCACCACTTTCCTGCTTTTCACTTATGCAAAATACTTAGGCGTTTCCAAGCAAGTGTTGAAATGCGGGAACATCATAGCCACTCCAACCCGAATA
CGTACTCTTGCAAAAAGACAATCACTGAGGAGTGACCGTTATATAAATATGGATTTGAGGTGCAAAGGGTGCTGTTAGGCTTGCTGCTGATGATCATTTTATAAATTGTATTTCTTAACACGTGGACTACATACTAGGAGACAACCCACTGCGAATGTCAAACATGGTGGTTTACGGTGCCAAGTTTCCACAGCATATAC
ATCACAGGGGCTCGTCGTTTCCTTCCATTTACAACCACCTCCAAACCATTCCCTGCAACGATGGACTTACATCTGGTAGCATGGCACATTTGTTGTTTTAAATGTTTTAAAACTATTGGCCTTATAATTAATGCTTCAACGTTCAAAGTGACTGTTGCACGTCTCTATGGCACATGGCACAAACCCATCCATTTGGCAAA
GGAAAGAGTCTAGACTCTAAACCTTTAATCAATTCAAAGAAGAGTTCAATCTTTTAACCCATTTTAAGTATTCTTTCATTAAGTGAAAGAATATGATGCGAAAACTATAATGTAT

Intron 2 GTAATATTCT ... GTTGTTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	78.95	8	30	27
A2	86.53	5	31.8	13.2
A3	74.49	25	73	12.2
A4	68.57	11	24	11.2
A5	82.14	5	23	6.2
A6	75.58	208.3	645	150.5
A7	75.2	187.7	569	188.3
A8	73.86	256.3	724	295.2
A9	75.97	167.3	529	157.7
A10	74.18	141.3	406	148.8

>TRINITY_DN129319_c0_g1_i4 from sample A1 / Example spliced isoform

TTTCTATATTCACTTGGTTTTGCGCTTCGTTTTCTCTGCGGCATGAGGTAGGGTCTTTGCTCTTCAATGGAGGACGCACAGTATCATTCTGCAATAAACTTTTTGGTTTATTAAAATCTCTTATTTGTTTTTTACTCGGCTATTACTTGTTATTGTTACAGGCATCTTGGAGTAATTCCCGTGGATGATAAGCTTGAGAA
TTCAATCCTTCCCCCGAAAGATCGCTGACGTAGACGCGGAGGTCAATCATTTATTCTGACTCCTCTCCTCCTCTGCGACTATGAAACCCTGTTCGCCTGTATGCCTCCGCTCTCCCCCTATGCGACTATGAAACCCCGTTAGCGAGCAGTTATGGGCAGCCTAACTAGTTTTAACAATGCGTACGTATGTAACCAGTGTT
TCAAATCCCAGCGGAGTAGAGCGATCTGGTGAAGAAGGTTCGACGCATGTAAAGATGCCGGATGCTCCCCATACCAGCAGAATTGCCTCCATTTCATATAACCCCGTGGCGGTATT

>TRINITY_DN129319_c0_g1_i2 from sample A1 / Example unspliced isoform:

TTTCTATATTCACTTGGTTTTGCGCTTCGTTTTCTCTGCGGCATGAGGTAGGGTCTTTGCTCTTCAATGGAGGACGCACAGTATCATTCTGCAATAAACTTTTTGGTTTATTAAAATCTCTTATTTGTTTTTTACTCGGCTATTACTTGTTATTGTTACAGGCATCTTGGAGTAATTCCCGTGGATGATAAGCTTGAGAA
TTCAATCCTTCCCCCGAAAGATCGCTGACGTAGACGCGGAGGTCAATCATTTATTCTGACTCCTCTCCTCCTCTGCGACTATGAAACCCTGTTCGCCTGTATGCCTCCGCTCTCCCCCTATGCGACTATGAAACCCCGTTAGCGAGCAGTTATGGGCAGCCTAACTAGTTTTAACAATGCGTACGTATGTAACCAGTGTT
TCAAATCCCAGCGGAGTAGAGCGATCTGGTGAAGAAgtaatattctctgatcctcgaatcttatctcaatgaaaaataagtactattctgtgatttgtgaaccgtatctcattgaaaaagcgacagaatggcgtcgctgattgtatggagttttatgtgttatttaaatgatataattcctctttatcgccaaaattcaa
atttcacatattgttgttttagGGTTCGACGCATGTAAAGATGCCGGATGCTCCCCATACCAGCAGAATTGGCTCCATTTCTTATAACCCCGTGACGGTATTTTAATTCACAATATTATTGACAGAATGTGCTCTCCTCTTGTAATTTTAACTTAGGCTTTTCGTAGAATATGGAACTTGAGGAGTTTGGTGGAGATCCC
CAAAATTCAAATTTCACATATTGTTGTTTTAGGGTTCGACAATACGATGCTCCCCATACCAGCAGAATTCGATCCATGTCATATAACCCCGTGGCGGTATTTTAATTCACAATATTTCTGGTAGAATGTGTTCTCTGCTTGTAATTTTTACTTACGGTTTTCGTAGAATATGGAACTATGAGGAATTTGGTGAAATCGCC
CAAATTCAAATTTCACATAGCTGCGGTCTATTTTAATTATTATGATCTCTTTAAATTATTATTTCGGTGTTGATAACCTTCTTATCCAACGAATGCAACGATATTCCTTGCTTTCTTCCATAATAAAATTTTAGTGAACTAATAAGTCATGGCTATCTTTTTTTTAGGACTCATTGGACAATGCTCGGGTGACATTCTTC
TTATCCGTGCAAAACAAAGATTAATGCGTTATGAAAGTTTAAATTTAAATCATTATGTATATGTATTTTGATATATGTATTTTGAGAAATTTATAAGCCATTGTTTGATATATGTATTTTGTTCTTTTAGTTTTTCTACTCTAATGTGACCACCTTATGTAAAAAGGCAGTAGGATATTAAATTAGAAAGCGGTAACATA
TTCAGTACTGTTTATTACAGGGAAAGCAACACTTACATAATCATTGTTATCATAATGGCAAATTAATTTAAAATATATGTAATTGTAATGACATCTGAGGTTTTTTCTTTATTTTTCTTTTTGTTCCAATTATTTTTTTTGGAAGTAAATTATCCATAACCAAAGATAAAATGAAAGGAAATGAAAACAAATTTATATTC
TTATGTAAAATGAAATGAAACAAAAACAAATTTGTTTCTATATATATGGAAAAGCCATTTTTTAATTGAAAATCAAAATTTTTAATACATTTTTCTTTAGCAGTTAGCACTTCGATTATTTAAGAGCAATGGAAAAATAATGGAAAAGGAAAATAGTTATGCGAAAGTAAAAACAAAGATGGAATCATTCATAGTTATAG
TAGAAACACAATAAAACTTGTAGTAGAAACACAATAAAACTTGTAGTAGAAACACAATAAAACTTG

Intron 3 GCCGAGGGTT ... AGATGTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.31	95	42	76
A2	44.09	123	57	137
A3	44.77	153	92	156
A4	48.35	86	67	94
A5	46.25	86	55	93
A6	45.51	622	311	728
A7	43.58	1010	436	1124
A8	41.96	935	394	958
A9	42.12	722	306	745
A10	46.2	726	411	836

>TRINITY_DN129319_c0_g1_i8 from sample A1 / Example spliced isoform

CAGCTTCTTCCCGATGAAGAGACCTCAGCCTGGTGTTTTTATCATTTCTATTCGCTGAGGGTTTTCGAGAGGTCCAGGCCATGCTGAAGGAGGTCGCCAAAGGTTTTCAAGATGTCGAGGCCATGCTGAAGGAGGTCGCTATGCTGAAGGAGGTCGCCGAGGGTTTTCAGGTCACCGAGGGTTTTCAAGATGTCCAGGCC
ATGCTGAAGGAGGATTTGTTGCAAGCGCATTCGCCTAGATCAATGGGATTGAGAGAGAGAACGCCCTCTTACTGTGTCCTGCGCGGATATTATTCAGATCTT

>TRINITY_DN129319_c0_g1_i13 from sample A1 / Example unspliced isoform:

GTCGAGGCCATGCTGAAGGAGGTCgccgagggttttcaagatgtccagGCTATGCTGAAGGAGGTCGCCGAGGGTTTTCAGGTCACCGAGGGTTTTCAAGATGTCCAGGCCATGCTAAAGGAGGATTTGTTGCAAGCGCATTCGCCTAGATCGATGCGATTAAGAGAGTCATATGCTTGATCTCACTCAAGTCGAATCAC
GATGCAGAGCAGAGCACGGTTATCCCTGACATGCGAGGAGGCGAGAACATTCTAACGGTTTGACGAATGTAAAAATGTCGGATGCTCCCCATACCAGCAGAATTG

Intron 4 GTAAATTCAT ... GTTGCTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.22	63.4	23.4	34.3
A2	19.5	58.5	24.1	13.5
A3	36.36	65.9	13.8	35.8
A4	39.96	44.8	21.5	28.6
A5	32.28	43.9	4.5	19.5
A6	27.02	565.1	127	198.9
A7	28.69	870.9	174.6	330.5
A8	28.3	1019.6	188	373.6
A9	20.08	717.3	105.1	171
A10	29.97	620.9	139.5	255.4

>TRINITY_DN129319_c0_g1_i13 from sample A1 / Example spliced isoform

GTCGAGGCCATGCTGAAGGAGGTCGCCGAGGGTTTTCAAGATGTCCAGGCTATGCTGAAGGAGGTCGCCGAGGGTTTTCAGGTCACCGAGGGTTTTCAAGATGTCCAGGCCATGCTAAAGGAGGATTTGTTGCAAGCGCATTCGCCTAGATCGATGCGATTAAGAGAGTCATATGCTTGATCTCACTCAAGTCGAATCAC
GATGCAGAGCAGAGCACGGTTATCCCTGACATGCGAGGAGGCGAGAACATTCTAACGGTTTGACGAATGTAAAAATGTCGGATGCTCCCCATACCAGCAGAATTG

>TRINITY_DN129319_c0_g1_i3 from sample A1 / Example unspliced isoform:

GTCGAGGCCATGCTGAAGGAGGTCGCCGAGGGTTTTCAAGATGTCCAGGCTATGCTGAAGGAGGTCGCCGAGGGTTTTCAGGTCACCGAGGGTTTTCAAGATGTCCAGGCCATGCTAAAGGAGGATTTGTTGCAAGCGCATTCGCCTAGATCGATGCGATTAAGAGAGATAACGCCCTCTTACTGTGTCCTGCGCGGATA
TTATTCAGATCTTTTATTTTTGATATTATTCAGATCTTTTATTTTTGTTGATATTGGTTTGATGTTTGAATGTTAAAATGCATACAGTCATATGCTTGATCTCACTCAAGTCGAATCACGATGCAGAGCAGAGCACGGTTATCCCTGACATGCGAGGAGGCGAGAACATTCTAACgtaaattcatgcttctttttctcat
ttcgatttgtgtccagccgtcccgatgaccctattatttggtttctcaaacctcatttcaatatctgtttctatttgctttcttatctccttgacatttgtgtattgcgattgttgctttagGGTTTGACGAATGTAAAAATGTCGGATGCTCCCCATACCAGCAGAATTGGCTCCATTTCTTATAACCCCGTGACGGTA
TTTTAATTCACAATATTATTGACAGAATGTGCTCTCCTCTTGTAATTTTAACTTAGGCTTTTCGTAGAATATGGAACTTGAGGAGTTTGGTGGAGATCCCCAAAATTCAAATTTCACATATTGTTGTTTTAGGGTTCGACAATACGATGCTCCCCATACCAGCAGAATTCGATCCATGTCATATAACCCCGTGGCGGTAT
TTTAATTCACAATATTTCTGGTAGAATGTGTTCTCTGCTTGTAATTTTTACTTACGGTTTTCGTAGAATATGGAACTATGAGGAATTTGGTGAAATCGCCCAAATTCAAATTTCACATAGCTGCGGTCTATTTTAATTATTATGATCTCTTTAAATTATTATTTCGGTGTTGATAACCTTCTTATCCAACGAATGCAACG
ATATTCCTTGCTTTCTTCCATAATAAAATTTTAGTGAACTAATAAGTCATGGCTATCTTTTTTTTAGGACTCATTGGACAATGCTCGGGTGACATTCTTCTTATCCGTGCAAAACAAAGATTAATGCGTTATGAAAGTTTAAATTTAAATCATTATGTATATGTATTTTGATATATGTATTTTGAGAAATTTATAAGCCA
TTGTTTGATATATGTATTTTGTTCTTTTAGTTTTTCTACTCTAATGTGACCACCTTATGTAAAAAGGCAGTAGGATATTAAATTAGAAAGCGGTAACATATTCAGTACTGTTTATTACAGGGAAAGCAACACTTACATAATCATTGTTATCATAATGGCAAATTAATTTAAAATATATGTAATTGTAATGACATCTGAGG
TTTTTTCTTTATTTTTCTTTTTGTTCCAATTATTTTTTTTGGAAGTAAATTATCCATAACCAAAGATAAAATGAAAGGAAATGAAAACAAATTTATATTCTTATGTAAAATGAAATGAAACAAAAACAAATTTGTTTCTATATATATGGAAAAGCCATTTTTTAATTGAAAATCAAAATTTTTAATACATTTTTCTTTAG
CAGTTAGCACTTCGATTATTTAAGAGCAATGGAAAAATAATGGAAAAGGAAAATAGTTATGCGAAAGTAAAAACAAAGATGGAATCATTCATAGTTATAGTAGAAACACAATAAAACTTGTAGTAGAAACACAATAAAACTTGTAGTAGAAACACAATAAAACTTG

Intron 5 GTATTTTAAT ... GTTGTTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.23	26	65	52
A2	70.09	16	34	41
A3	63.89	26	57	35
A4	57.5	17	29	17
A5	61.54	15	32	16
A6	70.42	181	454	408
A7	69.32	281	733	537
A8	69.6	418	978	936
A9	69	228	498	517
A10	74.04	156	483	407

>TRINITY_DN43012_c1_g1_i7 from sample A9 / Example spliced isoform

TTAAAATGCATACAGTCATATGCTTGATCTCACTCAAGTCGAATCACGATGCAGAGCAGAGCACGGTTATCCCTGACATGCGAGGAGGCGAGAACATTCTAACGGTTTGACGAATGTAAAAATGTCGGATGCTCCCCATACCAGCAGAATTGGCTCCATTTCTTATAACCCCGTGACGGGTTCGACAATACGATGCTCCC
CATACCAGCAGAATTCGATCCATGTCATATAACCCCGTGGCGGTATTTTAATTCACAATATTTCTGGTAGAATGTGTTCTCTGCTTGTAATTTTTACTTACGGTTTTCGTAGAATATGGAACTATGAGGAATTTGGTGAAATCGCCCAAATTCAAATTTCACATAGCTGCGGTCTATTTTAATTATTATGATCTCTTTAA
ATTATTATTTCGGTGTTGATAACCTTCTTATCCAACGAATGCAACGATATTCCTTG

>TRINITY_DN43012_c1_g1_i4 from sample A9 / Example unspliced isoform:

TTAAAATGCATACAGTCATATGCTTGATCTCACTCAAGTCGAATCACGATGCAGAGCAGAGCACGGTTATCCCTGACATGCGAGGAGGCGAGAACATTCTAACGGTTTGACGAATGTAAAAATGTCGGATGCTCCCCATACCAGCAGAATTGGCTCCATTTCTTATAACCCCGTGACGgtattttaattcacaatattat
tgacagaatgtgctctcctcttgtaattttaacttaggcttttcgtagaatatggaacttgaggagtttggtggagatccccaaaattcaaatttcacatattgttgttttagGGTTCGACAATACGATGCTCCCCATACCAGCAGAATTCGATCCATGTCATATAACCCCGTGGCGGTATTTTAATTCACAATATTTCT
GGTAGAATGTGTTCTCTGCTTGTAATTTTTACTTACGGTTTTCGTAGAATATGGAACTATGAGGAATTTGGTGAAATCGCCCAAATTCAAATTTCACATAGCTGCGGTCTATTTTAATTATTATGATCTCTTTAAATTATTATTTCGGTGTTGATAACCTTCTTATCCAACGAATGCAACGATATTCCTTG

Intron 6 GTAGGGTCTT ... ATTGTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	56.52	30	4	39
A2	54.88	37	2	45
A3	64.02	34.3	2	61
A4	57.63	25	3	34
A5	67.98	7.6	4	16
A6	57.33	311.9	18	419
A7	56.79	337	28	443
A8	55.83	377.4	18	477
A9	53.56	324.3	6	374
A10	56.21	288.3	21	370

>TRINITY_DN129319_c0_g1_i11 from sample A1 / Example spliced isoform

TTTCTATATTCACTTGGTTTTGCGCTTCGTTTTCTCTGCGGCATGAGGCATCTTGGAGTAATTCCCGTGGATGATAAGCTTGAGAATTCAATCCTTCCCCCGAAAGATCGCTGACGTAGACGCGGAGGTCAATCATTTATTCTGACTCCTCTCCTCCTCTGCGACTATGAAACCCTGTTCGCCTGTATGCCTCCGCTCTC
CCCCTATGCGACTATGAAACCCCGTTAGCGAGCAGTTATGGGCAGCCTAACTAGTTTTAACAATGCGTACGTATGTAACCAGTGTTTCAAATCCCAGCGGAGTAGAGCGATCTGGTGAAGAAGGTTCGACGCATGTAAAGATGCCGGATGCTCCCCATACCAGCAGAATTGCCTCCATTTCATATAACCCCGTGGCGGTA
TT

>TRINITY_DN129319_c0_g1_i4 from sample A1 / Example unspliced isoform:

TTTCTATATTCACTTGGTTTTGCGCTTCGTTTTCTCTGCGGCATGAGgtagggtctttgctcttcaatggaggacgcacagtatcattctgcaataaactttttggtttattaaaatctcttatttgttttttactcggctattacttgttattgttacagGCATCTTGGAGTAATTCCCGTGGATGATAAGCTTGAGAA
TTCAATCCTTCCCCCGAAAGATCGCTGACGTAGACGCGGAGGTCAATCATTTATTCTGACTCCTCTCCTCCTCTGCGACTATGAAACCCTGTTCGCCTGTATGCCTCCGCTCTCCCCCTATGCGACTATGAAACCCCGTTAGCGAGCAGTTATGGGCAGCCTAACTAGTTTTAACAATGCGTACGTATGTAACCAGTGTT
TCAAATCCCAGCGGAGTAGAGCGATCTGGTGAAGAAGGTTCGACGCATGTAAAGATGCCGGATGCTCCCCATACCAGCAGAATTGCCTCCATTTCATATAACCCCGTGGCGGTATT

Intron 7 GTAAATTCAT ... TTGCTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.8	37.9	29	8
A2	61	17.7	27	3
A3	49.68	12.3	12	4
A4	76.52	9.6	31	19
A5	29.56	14.4	6	4.8
A6	51.58	120.1	127	38.8
A7	43.74	238.9	183	37.4
A8	46.57	218.8	188	50.3
A9	45.28	140.4	114.7	31.6
A10	47.79	149.6	136	79.3

>TRINITY_DN48080_c0_g1_i4 from sample A7 / Example spliced isoform

GAGGTCGAGGCCATGCTGAAGGAAGTCGCGGGTTTTCGAGAGGTCGAGGCCATGCTGAAGGAGGTCGCCGAGGGTTTTCAAGATGTTCAGGCTATGCTGAAGGAGGTCGCCGAGGGTTTTCAGGTCACCGAGGGTTTTCAAGATGTCCAGGCCATGCTGAAGGAGGATTTGTTGCAAGCGCATTCGCCTAGATCGATGCG
ATTAAGAGAGATAACGCCCTCTTACTGTGTCCTGCGCGGATATTATTCAGATCTTCTATTTTTGTTGATATTGGTTTGATGTTTGAATGTTAAAATGGATAAAGTCATATGCAGGATCTCACTCAAGGCGAATCACGATGCAGAGCAGAGCGCGGTTATCCCTGACATGCGAGGAGGCGAGAACATTATCACATCAAGAT
TATAAACCCCTTGATGAACACAAGGGCCACGCCGACAACTTTATTTGCTCTCTCTTGCCGGGAACACCATCTTCGCAGGCGCAGTGCACATTTACACTAATTTATTTTATTTTAGTTTTGCAGGTGGACTGCTCTTCCAAATGAGTGATAGCAATTTGTAATATGTGACGTCCACCACTTTCCTGCTTTTCACTTATGCA
AAATACTTAGGCGTTTCCAAGCAAGTGTTGAAATGCGGGAACATCATAGCCACTCCAACCCGAATACGTACTCTTGCAAAAAGACAATCACTGAGGAGTGACCGTTATATAAATATGGATTTGAGGTGCAAAGGGTGCTGTTAGGCTTGCTGCTGATGATCATTTTATAAATTGTATTTCTTAACACGTGGACTACATAC
TAGGAGACAACCCACTGCGAATGTCAAACATGGTGGTTTACGGTGCCAAGTTTCCACAGCATATACATCACAGGGGCTCGTCGTTTCCTTCCATTTACAACCACCTCCAAACCATTCCCTGCAACGATGGACTTACATCTGGTAGCATGGCACATTTGTTGTTTTAAATGTTTTAAAACTATTGGCCTTATAATTAATGC
TTCAACGTTCAAAGTGACTGTTGCACGTCTCTATGGCACATGGCACAAACCCATCCATTTGGCAAAGGAAAGAGTCTAGACTCTAAACCTTTAATCAATTCAAAGAAGAGTTCAATCTTTTAACCCATTTTAAGTATTCTTTCATTAAGTGAAAGAATATGATGCGAAAACTATAATGTATGTTTAGATTAAAATATTTA
TTAAATAATATAATTTTTTATTCTATTGAAATAGTTAAA

>TRINITY_DN48080_c0_g1_i19 from sample A7 / Example unspliced isoform:

GAGGTCGAGGCCATGCTGAAGGAAGTCGCGGGTTTTCGAGAGGTCGAGGCCATGCTGAAGGAGGTCGCCGAGGGTTTTCAAGATGTCCAGGCTATGCTGAAGGAGGTCGCCGAGGGTTTTCAGGTCACCGAGGGTTTTCAAGATGTCCAGGCCATGCTGAAGGAGGATTTGTTGCAAGCGCATTCGCCTAGATCGATGCG
ATTAAGAGAGATAACGCCCTCTTACTGTGTCCTGCGCGGATATTATTCAGATCTTCTATTTTTGTTGATATTGGTTTGATGTTTGAATGTTAAAATGGATAAAGTCATATGCAGGATCTCACTCAAGGCGAATCACGATGCAGAGCAGAGCGCGGTTATCCCTGACATGCGAGGAGGCGAGAACATTATCACgtaaattc
atgcttctttttctcatttcgatttgtgtccacccgtccctattatttgatttctcaaaccttattttaatatctgtttctatttgctttcctatctctttgacatctatgtattgcgattgttgctttcagATCAAGATTATAAACCCCTTGATGAACACAAGGGCCACGCCGACAACTTTATTTGCTCTCTCTTGCCG
GGAACACCATCTTCGCAGGCGCAGTGCACATTTACACTAATTTATTTTATTTTAGTTTTGCAGGTGGACTGCTCTTCCAAATGAGTGATAGCAATTTGTAATATGTGACGTCCACCACTTTCCTGCTTTTCACTTATGCAAAATACTTAGGCGTTTCCAAGCAAGTGTTGAAATGCGGGAACATCATAGCCACTCCAACC
CGAATACGTACTCTTGCAAAAAGACAATCACTGAGGAGTGACCGTTATATAAATATGGATTTGAGGTGCAAAGGGTGCTGTTAGGCTTGCTGCTGATGATCATTTTATAAATTGTATTTCTTAACACGTGGACTACATACTAGGAGACAACCCACTGCGAATGTCAAACATGGTGGTTTACGGTGCCAAGTTTCCACAGC
ATATACATCACAGGGGCTCGTCGTTTCCTTCCATTTACAACCACCTCCAAACCATTCCCTGCAACGATGGACTTACATCTGGTAGCATGGCACATTTGTTGTTTTAAATGTTTTAAAACTATTGGCCTTATAATTAATGCTTCAACGTTCAAAGTGACTGTTGCACGTCTCTATGGCACATGGCACAAACCCATCCATTT
GGCAAAGGAAAGAGTCTAGACTCTAAACCTTTAATCAATTCAAAGAAGAGTTCAATCTTTTAACCCATTTTAAGTATTCTTTCATTAAGTGAAAGAATATGATGCGAAAACTATAATGTATGTTTAGATTAAAATATTTATTAAATAATATAATTTTTTATTCTATTGAAATAGTTAAA

Intron 8 GTACAATCAC ... TTCTTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.18	229	23	72
A2	14.39	241	26	55
A3	15.81	253	29	66
A4	14.68	215	19	55
A5	9.66	159	9	25
A6	15.59	1546	228	343
A7	22.64	943	240	312
A8	23.49	1249	320	447
A9	19.14	1153	248	298
A10	25.3	797	226	314

>TRINITY_DN129319_c0_g1_i12 from sample A1 / Example spliced isoform

AGACCTCAGCCTGGTGTTTTTATCATTTCTATTCGCTGAGGGTTTTCGAGAGGTCGAGGCCATGCTGAAGGAGGTCGCCGAGGGTTTTCAAGATGTCCAGGCTATGCTGAAGGAGGTCGCCGAGGGTTTTCAGGTCGCCATGCCAGGCAAGCGCATTCGCCTAGATCGATACGATTGAGAGAGATAACGCTCTCTTACTG
TGTGATGCAATATGTGACGTCCACAACTTTCCTGCTTTTCACTAATGCAAAATACTTAAGCGTTTCCAAGCAAGTGTTGAGATGTGGGAACATCAATGCCACTCCAACCCGAATACATACTCTTGCAAAAAGACAGGTGGACTACATACTAGGAGACAACCCACTGCGAATGCCATACATGGTGGTTTACGGTGCCAAGT
TTCCACAGCATATACATCACAGGGGCTCGTCGTTGCCTTCCATTTACAACCACCTCCGAACCATTGCCTGCAACGATGGATTCACATATGGTAGCATGGCGCATCTGTTGTTGTAAATGTTGTAAGTGACTTGCACGTCTCTATGGCACATGGCCGCACGTCATGGTATTTATTTTTAAAGTTCATTAGCTAACTGTGGA
CAAATACAAACCCATCCATTTGGCAAAGGAAGGAGTCTAGACTCTAAACGTTTAATCAATTCAAAGAAGAGTTTAATTTTTTTTAACCCAAT

>TRINITY_DN129319_c0_g1_i18 from sample A1 / Example unspliced isoform:

AGACCTCAGCCTGGTGTTTTTATCATTTCTATTCGCTGAGGGTTTTCGAGAGGTCGAGGCCATGCTGAAGGAGGTCGCCGAGGGTTTTCAAGATGTCCAGGCTATGCTGAAGGAGGTCGCCGAGGGTTTTCAGGTCGCCATGCCAGGCAAGCGCATTCGCCTAGATCGATACGATTGAGAGAGATAACGCTCTCTTACTG
TGTGATGCAATATGTGACGTCCACAACTTTCCTGCTTTTCACTAATGCAAAATACTTAAGCGTTTCCAAGCAAGTGTTGAGATGTGGGAACATCAATGCCACTCCAACCCGAATACATACTCTTGCAAAAAGACAGgtacaatcactgagtagtgaccgtacgttatataaatatggatttgaggcgcaaagggtgctgt
taggctttctgctgatgatcattttataaattgtatttcttaacagGTGGACTACATACTAGGAGACAACCCACTGCGAATGCCATACATGGTGGTTTACGGTGCCAAGTTTCCACAGCATATACATCACAGGGGCTCGTCGTTGCCTTCCATTTACAACCACCTCCGAACCATTGCCTGCAACGATGGATTCACATATG
GTAGCATGGCGCATCTGTTGTTGTAAATGTTGTAAGTGACTTGCACGTCTCTATGGCACATGGCCGCACGTCATGGTATTTATTTTTAAAGTTCATTAGCTAACTGTGGACAAATACAAACCCATCCATTTGGCAAAGGAAGGAGTCTAGACTCTAAACGTTTAATCAATTCAAAGAAGAGTTTAATTTTTTTTAACCCA
AT