sp|P07591|TRXM_SPIOL ----> Thioredoxin M-type, chloroplastic

Intron 1 GTCTTGATCT ... TGTGTTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	63.41	30	54	50
A2	46.77	33	25	33
A3	57.08	39.8	61.8	44
A4	76.62	10.7	26.7	43
A5	75.31	10	35	26
A6	56.34	86.5	80.3	143
A7	51.39	140	102	194
A8	51.44	101	67	147
A9	47.18	152.7	107.7	165
A10	51.86	136	125	168

>TRINITY_DN121384_c7_g1_i3 from sample A2 / Example spliced isoform

GCCAGAATATAGTGGTCACCCAAACGAAAGAACACATGAAAAAAAGGAGATCATGCGGTTCTGAGTCCAAAGGCAGCGGGTCTTCCAATAGAAACAGTGGGTATCTCCTCCCCCCTAACAGCCTCCAGGCCTTCTTCCTGCACATCCAATACAGTCGCCTGTGCATCGGTCCTACGCGGCAGGAGGAGTTTCAACAGAAA
GAACTTATACCTGCTACCCTCATGCAGAGGATTGAGGATTGAGGGTCATGATAACAGGGGTCTGCCTGTTTCCTCCAATGTAAAAAGGACGCGTGGCTTCAAGAGCGTTGTCTGTGAGGCACAAGAGACAGTTGCCCAAGTTCAGGATGTCAATGATGGGACATGGAAGAATCTTGTCTTAGACAGTGATATTCCCGTGC
TTGTTGACTTTTGGGCACCATGGTGTGGACCTTGTCGAATGATAGAACCCCTGATAGATGAACTTGCAAAACAGTATTCTGGAAAGATAAAATGCCTTAAACTCAATACCGATGAAAGTCCACGTATTGCAACTGAATATGGCATCAGGAGCATTCCAACTGTCATGATATTTAAGAATGGAGAGAAGAAAGACACTGTA
ATTGGAGCTGTGCCCAAGACTACTTTAACTGCAAGCATTGAAAAATACGTGCAGTGGTAAAAAGTAAGAATTTGGAAGATGTCTTGCTTGGAATAAGCCCTATTTACTATCTTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAGTAAGAATTTGGAAGATGTCTTGCTTGGAATAAGCCCTATTTACTATC
TTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAG

>TRINITY_DN121384_c7_g1_i4 from sample A2 / Example unspliced isoform:

GCCAGAATATAGTGGTCACCCAAACGAAAGAACACATGAAAAAAAGGAGATCATGCGGTTCTGAGTCCAAAGGCAGCGGGTCTTCCAATAGgtcttgatctgaatcgaatatagtttccttgaacgttgccaattatcacagatttcgtctttgggttgttctaaatttgtttgctcagtccagatgtacgtgtggcttg
gggtttgatctgagcatttacctggtttttatcttttgggttattggctatcgtggatttaattccttgggtactacaacagtccgcaatttagcctgctcgtggcacatatcccgatttgggttctccaaaattagtctgttttaccattttgattgtaggtttccttgggtccctataaagcggagaattagcctgtt
ttttccgtttgggtgattggttattgcagatttcctcacctgggtcctagattttaggccattttgtagagattttactgtactaattgtctattgtttgatctgcaatggcttgtgttctagAAACAGTGGGTATCTCCTCCCCCCTAACAGCCTCCAGGCCTTCTTCCTGCACATCCAATACAGTCGCCTGTGCATCG
GTCCTACGCGGCAGGAGGAGTTTCAACAGAAAGAACTTATACCTGCTACCCTCATGCAGAGGATTGAGGATTGAGGGTCATGATAACAGGGGTCTGCCTGTTTCCTCCAATGTAAAAAGGACGCGTGGCTTCAAGAGCGTTGTCTGTGAGGCACAAGAGACAGTTGCCCAAGTTCAGGATGTCAATGATGGGACATGGAA
GAATCTTGTCTTAGACAGTGATATTCCCGTGCTTGTTGACTTTTGGGCACCATGGTGTGGACCTTGTCGAATGATAGAACCCCTGATAGATGAACTTGCAAAACAGTATTCTGGAAAGATAAAATGCCTTAAACTCAATACCGATGAAAGTCCACGTATTGCAACTGAATATGGCATCAGGAGCATTCCAACTGTCATGA
TATTTAAGAATGGAGAGAAGAAAGACACTGTAATTGGAGCTGTGCCCAAGACTACTTTAACTGCAAGCATTGAAAAATACGTGCAGTGGTAAAAAGTAAGAATTTGGAAGATGTCTTGCTTGGAATAAGCCCTATTTACTATCTTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAGTAAGAATTTGGAAGA
TGTCTTGCTTGGAATAAGCCCTATTTACTATCTTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAG

Intron 2 GTCTTGATCT ... CTAGAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.35	32	54	52
A2	52.63	27	25	35
A3	57.14	39	59	45
A4	79.07	9	24	44
A5	71.11	13	35	29
A6	54.9	92	74	150
A7	49.67	153	102	200
A8	49.77	109	67	149
A9	46.63	156.5	104.5	169
A10	50.68	146	125	175

>TRINITY_DN111768_c0_g1_i3 from sample A1 / Example spliced isoform

ATATAGTGGTCACCCAAACGAAAGAACACATGAAAAAAAGGAGATCATGCGGTTCTGAGTCCAAAGGCAGCGGGTCTTCCAATAGTGGGTATCTCCTCCCCCCTAACAGCCTCCAGGCCTTCTTCCTGCACATCCAATACAGTCGCCTGTGCATCGGTCCTACGCGGCAGGAGGAGTTTCAACAGAAAGAACTTATACCT
GCTACCCTCATGCAGAGGATTGAGGATTGAGGGTCATGATAACAGGGGTCTGCCTGTTTCCTCCAATGTAAAAAGGACGCGTGGCTTCAAGAGCGTTGTCTGTGAGGCACAAGAGACAGTTGCCCAAGGTAGGTATTCAGTTCAGGATGTCAATGATGGGACATGGAAGAATCTTGTCTTAGACAGTGATATTCCCGTGC
TTGTTGACTTTTGGGCACCATGGTGTGGACCTTGTCGAATGATAGAACCCCTGATAGATGAACTTGCAAAACAGTATTCTGGAAAGATAAAATGCCTTAAACTCAATACCGATGAAAGTCCACGTATTGCAACTGAATATGGCATCAGGAGCATTCCAACTGTCATGATATTTAAGAATGGAGAGAAGAAAGACACTGTA
ATTGGAGCTGTGCCCAAGACTACTTTAACTGCAAGCATTGAAAAATACGTGCAGTGGTAAAAAGTAAGAATTTGGAAGATGTCTTGCTTGGAATAAGCCCTATTTACTATCTTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAGTAAGAATTTGGAAGATGTCTTGCTTGGAATAAGCCCTATTTACTATC
TTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAGGATGCCTGAAGAGATTGGTGGTATTTTTGTATATATATGTAGGTCAATTCTGTATTGTTCTTTGCTATGAAATGAAATGTCCTGTAGAGAAATATACTTGAATTTTCTTTTTTTTCACAATATTTTTGTAGCTATGAATGCAAAATTGTGGAAACTCA
AAACTTTCACTGAGAATCTTAAAATAGGCTTGTTATATGCATATTTCATCTACAAGAAACTGAACTAATGAATGGAAAGGTGAAAAATCCGTCAGATCTTTTGATTATGTGCAGGCCATTCTTTGCATTCAGTTTGCCACTGAATCCCAGCATTTTGTCAA

>TRINITY_DN111768_c0_g1_i2 from sample A1 / Example unspliced isoform:

ATATAGTGGTCACCCAAACGAAAGAACACATGAAAAAAAGGAGATCATGCGGTTCTGAGTCCAAAGGCAGCGGGTCTTCCAATAGgtcttgatctgaatcgaatatagtttccttgaacgttgccaattatcacagatttcgtctttgggttgttctaaatttgtttgctcagtccagatgtacgtgtggcttggggttt
gatctgagcatttacctggtttttatcttttgggttattggctatcgtggatttaattccttgggtactacaacagtccgcaatttagcctgctcgtggcacatatcccgatttgggttctccaaaattagtctgttttaccattttgattgtaggtttccttgggtccctataaagcggagaattagcctgttttttcc
gtttgggtgattggttattgcagatttcctcacctgggtcctagattttaggccattttgtagagattttactgtactaattgtctattgtttgatctgcaatggcttgtgttctagaaacagTGGGTATCTCCTCCCCCCTAACAGCCTCCAGGCCTTCTTCCTGCACATCCAATACAGTCGCCTGTGCATCGGTCCTA
CGCGGCAGGAGGAGTTTCAACAGAAAGAACTTATACCTGCTACCCTCATGCAGAGGATTGAGGATTGAGGGTCATGATAACAGGGGTCTGCCTGTTTCCTCCAATGTAAAAAGGACGCGTGGCTTCAAGAGCGTTGTCTGTGAGGCACAAGAGACAGTTGCCCAAGGTAGGTATTCAGTTCAGGATGTCAATGATGGGAC
ATGGAAGAATCTTGTCTTAGACAGTGATATTCCCGTGCTTGTTGACTTTTGGGCACCATGGTGTGGACCTTGTCGAATGATAGAACCCCTGATAGATGAACTTGCAAAACAGTATTCTGGAAAGATAAAATGCCTTAAACTCAATACCGATGAAAGTCCACGTATTGCAACTGAATATGGCATCAGGAGCATTCCAACTG
TCATGATATTTAAGAATGGAGAGAAGAAAGACACTGTAATTGGAGCTGTGCCCAAGACTACTTTAACTGCAAGCATTGAAAAATACGTGCAGTGGTAAAAAGTAAGAATTTGGAAGATGTCTTGCTTGGAATAAGCCCTATTTACTATCTTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAGTAAGAATTT
GGAAGATGTCTTGCTTGGAATAAGCCCTATTTACTATCTTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAGGATGCCTGAAGAGATTGGTGGTATTTTTGTATATATATGTAGGTCAATTCTGTATTGTTCTTTGCTATGAAATGAAATGTCCTGTAGAGAAATATACTTGAATTTTCTTTTTTTTCACAA
TATTTTTGTAGCTATGAATGCAAAATTGTGGAAACTCAAAACTTTCACTGAGAATCTTAAAATAGGCTTGTTATATGCATATTTCATCTACAAGAAACTGAACTAATGAATGGAAAGGTGAAAAATCCGTCAGATCTTTTGATTATGTGCAGGCCATTCTTTGCATTCAGTTTGCCACTGAATCCCAGCATTTTGTCAA

Intron 3 GTCTTGATCT ... GTTGCCCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.67	6	54	24
A2	71.01	10	25	24
A3	78.18	12	62	24
A4	71.76	12	27	34
A5	88.06	4	35	24
A6	66.47	58	81	149
A7	69.11	59	102	162
A8	71.68	49	67	181
A9	72.45	50	108	155
A10	77.48	34	125	109

>TRINITY_DN42791_c0_g2_i8 from sample A6 / Example spliced isoform

TCGGAATTCTATCCACACACAACGAGAACCTCATCCACACTGAATTTCATATACATATCGAATATAAAACCCAAAAATCTCCGCCAGAATATAGTGGTCACCCAAACGAAAGAACACATGAAAAAAAGGAGATCATGCGGTTCTGAATCCAAAGGCAGCGGGTCTTCCAATAGTTCAGGATGTCAATGATGGGACATGGA
AGAATCTTGTCTTAGACAGTGATATTCCCGTGCTTGTTGACTTTTGGGCACCATGGTGTGGACCTTGTCGAATGATAGAACCCCTGATAGATGAACTTGCAAAACAGTATTCTGGAAAGATAAAATGCCTTAAACTCAATACCGATGAAAGTCCACGTATTGCAACTGAATATGGCATCAGGAGCATTCCAACTGTCATG
ATATTTAAGAATGGAGAGAAGAAAGACACTGTAATTGGAGCTGTGCCCAAGACTACTTTAACTGCAAGCATTGAAAAATACGTGCAGTGGTAAAAAGTAAGAATTTGGAAGATGT

>TRINITY_DN42791_c0_g2_i2 from sample A6 / Example unspliced isoform:

TCGGAATTCTATCCACACACAACGAGAACCTCATCCACACTGAATTTCATATACATATCGAATATAAAACCCAAAAATCTCCGCCAGAATATAGTGGTCACCCAAACGAAAGAACACATGAAAAAAAGGAGATCATGCGGTTCTGAATCCAAAGGCAGCGGGTCTTCCAATAGgtcttgatctgaatcgaatatagtttc
cttgaacgttgccaattatcacagatttcgtctttgggttgttctaaatttgtttgctcagtccagatgtacgtgtggcttggggtttgatctgagcatttacctggtttttatcttttgggttattggctatcgtggatttaattccttgggtactacaacagtccgcaatttagcctgctcgtggcacatatcccgat
ttgggttctccaaaattagtctgttttaccattttgattgtaggtttccttgggtccctataaagcggagaattagcctgttttttccgtttgggtgattggttattgcagatttcctcacctgggtcctagattttaggccattttgtagagattttactgtactaattgtctattgtttgatctgcaatggcttgtgt
tctagaaacagtgggtatctcctcccccctaacagcctccaggccttcttcctgcacatccaatacagtcgcctgtgcatcggtcctacgcggcaggaggagtttcaacagaaagaacttatacctgctaccctcatgcagaggattgaggattgagggtcatgataacaggggtctgcctgtttcctccaatgtaaaaa
ggacgcgtggcttcaagagcgttgtctgtgaggcacaagagacagttgcccaagTTCAGGATGTCAATGATGGGACATGGAAGAATCTTGTCTTAGACAGTGATATTCCCGTGCTTGTTGACTTTTGGGCACCATGGTGTGGACCTTGTCGAATGATAGAACCCCTGATAGATGAACTTGCAAAACAGTATTCTGGAAAG
ATAAAATGCCTTAAACTCAATACCGATGAAAGTCCACGTATTGCAACTGAATATGGCATCAGGAGCATTCCAACTGTCATGATATTTAAGAATGGAGAGAAGAAAGACACTGTAATTGGAGCTGTGCCCAAGACTACTTTAACTGCAAGCATTGAAAAATACGTGCAGTGGTAAAAAGTAAGAATTTGGAAGATGTCTTG
CTTGGAATAAGCCCTATTTACTATCTTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAGTAAGAATTTGGAAGATGTCTTGCTTGGAATAAGCCCTATTTACTATCTTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAGGATGCCTGAAGAGATTGGTGGTATTTTTGTATATATATGTAGGT
CAATTCTGTATTGTTCTTTGCTATGAAATGAAATGTCCTGTAGAGAAATATACTTGAATTTTCTTTTTTTTCACAATATTTTTGTAGCTATGAATGCAAAATTGTGGAAACTCAAAACTTTCA

Intron 4 GTCTTGATCT ... AGGTATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.05	6	54	20
A2	70.59	10	25	23
A3	77.78	12	62	22
A4	70.73	12	27	31
A5	87.3	4	35	20
A6	63.41	58	81	120
A7	68.02	59	102	149
A8	70.66	49	67	169
A9	71.51	50	108	143
A10	76.95	34	125	102

>TRINITY_DN42791_c0_g2_i8 from sample A6 / Example spliced isoform

TCGGAATTCTATCCACACACAACGAGAACCTCATCCACACTGAATTTCATATACATATCGAATATAAAACCCAAAAATCTCCGCCAGAATATAGTGGTCACCCAAACGAAAGAACACATGAAAAAAAGGAGATCATGCGGTTCTGAATCCAAAGGCAGCGGGTCTTCCAATAGTTCAGGATGTCAATGATGGGACATGGA
AGAATCTTGTCTTAGACAGTGATATTCCCGTGCTTGTTGACTTTTGGGCACCATGGTGTGGACCTTGTCGAATGATAGAACCCCTGATAGATGAACTTGCAAAACAGTATTCTGGAAAGATAAAATGCCTTAAACTCAATACCGATGAAAGTCCACGTATTGCAACTGAATATGGCATCAGGAGCATTCCAACTGTCATG
ATATTTAAGAATGGAGAGAAGAAAGACACTGTAATTGGAGCTGTGCCCAAGACTACTTTAACTGCAAGCATTGAAAAATACGTGCAGTGGTAAAAAGTAAGAATTTGGAAGATGT

>TRINITY_DN42791_c0_g2_i5 from sample A6 / Example unspliced isoform:

TCGGAATTCTATCCACACACAACGAGAACCTCATCCACACTGAATTTCATATACATATCGAATATAAAACCCAAAAATCTCCGCCAGAATATAGTGGTCACCCAAACGAAAGAACACATGAAAAAAAGGAGATCATGCGGTTCTGAATCCAAAGGCAGCGGGTCTTCCAATAGgtcttgatctgaatcgaatatagtttc
cttgaacgttgccaattatcacagatttcgtctttgggttgttctaaatttgtttgctcagtccagatgtacgtgtggcttggggtttgatctgagcatttacctggtttttatcttttgggttattggctatcgtggatttaattccttgggtactacaacagtccgcaatttagcctgctcgtggcacatatcccgat
ttgggttctccaaaattagtctgttttaccattttgattgtaggtttccttgggtccctataaagcggagaattagcctgttttttccgtttgggtgattggttattgcagatttcctcacctgggtcctagattttaggccattttgtagagattttactgtactaattgtctattgtttgatctgcaatggcttgtgt
tctagaaacagtgggtatctcctcccccctaacagcctccaggccttcttcctgcacatccaatacagtcgcctgtgcatcggtcctacgcggcaggaggagtttcaacagaaagaacttatacctgctaccctcatgcagaggattgaggattgagggtcatgataacaggggtctgcctgtttcctccaatgtaaaaa
ggacgcgtggcttcaagagcgttgtctgtgaggcacaagagacagttgcccaaggtaggtattcagTTCAGGATGTCAATGATGGGACATGGAAGAATCTTGTCTTAGACAGTGATATTCCCGTGCTTGTTGACTTTTGGGCACCATGGTGTGGACCTTGTCGAATGATAGAACCCCTGATAGATGAACTTGCAAAACAG
TATTCTGGAAAGATAAAATGCCTTAAACTCAATACCGATGAAAGTCCACGTATTGCAACTGAATATGGCATCAGGAGCATTCCAACTGTCATGATATTTAAGAATGGAGAGAAGAAAGACACTGTAATTGGAGCTGTGCCCAAGACTACTTTAACTGCAAGCATTGAAAAATACGTGCAGTGGTAAAAAGTAAGAATTTG
GAAGATGTCTTGCTTGGAATAAGCCCTATTTACTATCTTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAGTAAGAATTTGGAAGATGTCTTGCTTGGAATAAGCCCTATTTACTATCTTGACACTGGAATTATCATGGCTGGATGTACTTTTGCAAAAGGATGCCTGAAGAGATTGGTGGTATTTTTGTAT
ATATATGTAGGTCAATTCTGTATTGTTCTTTGCTATGAAATGAAATGTCCTGTAGAGAAATATACTTGAATTTTCTTTTTTTTCACAATATTTTTGTAGCTATGAATGCAAAATTGTGGAAACTCAAAACTTTCA