sp|Q93VG8|PPDEX_ARATH ----> DeSI-like protein At4g17486

Intron 1 GTTCTTTCTG ... TTGTTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.78	42	100	94
A2	74.38	24	67.2	72.2
A3	72.83	25	67	67
A4	68.21	26	55.8	55.8
A5	73.33	12	35	31
A6	68.05	131	274	284
A7	71.59	113	285.2	284.4
A8	72.34	101	264.2	264.2
A9	72.06	105	261.8	279.8
A10	71.34	75	183.2	190.2

>TRINITY_DN41654_c0_g1_i1 from sample A8 / Example spliced isoform

ATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTATTGGTTTGGCGTGGGCATCTACCATTCTG
GTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCAT
CTCATTGCAAAAAATTGTAATCATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGAAAGTATTCAGGTTACAGCTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGATGGGTTAGAAGATGGGTCAGAATCCAGATCAACTGAAGACCAAATAGA
GAGTGATGGGGAGCAAGATCATCATCTACTAATTTCTTCCAATGGGGAGGCTGAGTCAATCGAAAGGGAAAATATATGGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCTCAAGCATGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGCTGGTTCATGATCTTTTTGCTGCTCAATTTC
TTCCATACTGAAGTTAGTGCATATAAACTACTTGGTATAGCCCTATTTCATAATAGAGCTATTACTTGGGTCAAGTTTCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTTCTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGGATTTCCAATAGGAAGTCTACCTTCACATTT
TGTGGCTGTAAACAATTAGTTCCGTTAGGTCAAATTCATCTGCAGTGTGTTTACTTTCTATGGGTTATGGAAGAAGAAATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTCTTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTGCATCATTTTGGAAATATCAATATTGTATGT
CTTTTATGGGGATAATAATTTTTCTTGTGCAGCT

>TRINITY_DN41654_c0_g1_i4 from sample A8 / Example unspliced isoform:

ATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTATTGGTTTGGCGTGGGCATCTACCATTCTG
GTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCAT
CTCATTGCAAAAAATTGTAATCATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGgttctttctgcaactgtttgttgccagAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGAAGATGGGTTAGAAGATGGGTC
AGAATCCAGATCAACTGAAGACCAAATAGAGAGTGATGGGGAGCAAGATCATCATCTACTAATTTCTTCCAATGGGGAGGCTGAGTCAATCGAAAGGGAAAATATATGGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCTCAAGCATGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGC
TGGTTCATGATCTTTTTGCTGCTCAATTTCTTCCATACTGAAGTTAGTGCATATAAACTACTTGGTATAGCCCTATTTCATAATAGAGCTATTACTTGGGTCAAGTTTCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTTCTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGG
ATTTCCAATAGGAAGTCTACCTTCACATTTTGTGGCTGTAAACAATTAGTTCCGTTAGGTCAAATTCATCTGCAGTGTGTTTACTTTCTATGGGTTATGGAAGAAGAAATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTCTTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTG
CATCATTTTGGAAATATCAATATTGTATGTCTTTTATGGGGATAATAATTTTTCTTGTGCAGCT

Intron 2 GTTACTATTT ... GATTGAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.44	14.3	16	21
A2	63.64	12	6	21
A3	58.11	35.3	11	49
A4	56.84	27.3	9	36
A5	62.96	10	3	17
A6	54.62	55.7	21	67
A7	53.39	87.3	13	100
A8	56.34	38	9	49
A9	52.01	75.7	16	82
A10	49.87	66.3	6	66

>TRINITY_DN39606_c0_g1_i5 from sample A9 / Example spliced isoform

GTGGTCCGTCACTACCCTTTTTGGTATCTTCTAATTCTCAAATTTAATTATTTTGGTTTGGTTTTCCAGTGATTTATGGCTGTTTTTTTAGAAAAATAAGAATCTACATTAAGTTTGTACTGTATTGTTTAACCTCCTGTGGATGGGATCAGAGATGCATACCGTCAATATCATCGATCTGATTTATCTCAGTTGAATTT
TTATTGCTGACTGATTGGTGTGCTACTGCTACTGGTGACCTGTTCGATTTCTCTCATTGCGTCTCTGTCTTCGTTTCTGTGATAGGCAGAATAGTTTCAGGTCTCTGTCAATCTCTGGTATCTGTTAATCTGATGTACACGCATTTGCCTGATTATGCCGCTGTTTGGTTTAGGAAAATGAAGAATTTGGTTTTAATATG
TCAACAGTAACTAAATTTGGGATCGGTTAGATCACCAGTTTAGCCTTTGCAGATTTGTGTGGGTTACGGGGAACCCTGAAGGATGGATACACAGATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGC
AGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTACTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCG
CTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAATCATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTTCTTTCT
GCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAG

>TRINITY_DN39606_c0_g1_i2 from sample A9 / Example unspliced isoform:

GTGGTCCGTCACTACCCTTTTTGGTATCTTCTAATTCTCAAATTTAATTATTTTGGTTTGGTTTTCCAGTGATTTATGGCTGTTTTTTTAGAAAAATAAGAATCTACATTAAGTTTGTACTGTATTGTTTAACCTCCTGTGGATGGGATCAGAGATGCATACCGTCAATATCATCGATCTGATTTATCTCAGTTGAATTT
TTATTGCTGACTGATTGGTGTGCTACTGCTACTGGTGACCTGTTCGATTTCTCTCATTGCGTCTCTGTCTTCGTTTCTGTGATAGGCAGAATAGTTTCAGGTCTCTGTCAATCTCTGGTATCTGTTAATCTGATGTACACGCATTTGCCTGATTATGCCGCTGTTTGGTTTAGGAAAATGAAGAATTTGGTTTTAATATG
TCAACAGTAACTAAATTTGGGATCGGTTAGATCACCAGTTTAGCCTTTGCAGgttactatttactgttatttgggttatttctctctgccagtgttgttaaggatttctctctgtggactccgattgttgtctttcttatgcttttgatttggggttgtagtgtatctgccaccagtttagctaagaatttcatattgtg
ggttcttcattttattccacggtttcacccgacaaattttttgtctgggattgagcagATTTGTGTGGGTTACGGGGAACCCTGAAGGATGGATAGACAAATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGAT
TCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTACTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATT
CAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAATCATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTT
CTTTCTGCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGTTTCTGCTGTAGAAGATGGGTTAGAAGATGGGTCAGAATCCAGATCAACTGAAGACCAAATAGAGAGTGATGGGGAGCAAGATCATCATCTACTAATTTCTTCCAATGGGGAGGCTGAGTCA
ATCGAAAGGGAAAATATATGGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCCCAAGCATGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGCTGGTTCATGATCTTTTTGCTGCTCAATTTCTTCCATACTGAAGTTAGTGCATATAAACTACTTGGTATAGCCCTATTTCATAATAGAG
CTATTACTTGGGTCAAGTTTCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTTCTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGGATTTCCAATAGGAAGTCTACCTTCACATTTTGTGGCTGTAAACAATTAGTTCCGTTAGGTCAAATTCATCTGCAGTGTGTTTACTTTC
TATGGGTTATGGAAGAAGAAATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTCTTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTGCATCATTTTGGAAATATCAATATTGTATGTCTTTTATGGGGCCAATAATTTTTCTTGTGCAGCTATTTCCTTTTTTCCATGTGAGCGT
TCATTTTTATCTAATCATTTATGAACTCTATGACTTGCTCTCTGCCTGAAACAGGCTTTGCTTTGAATTTTTATGGGCATGCCTTTGCATATCTGTGTGACAC

Intron 3 GTCTCTGTCA ... GCCTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.14	32	7	14.5
A2	29.73	52	32	12
A3	26.47	75	19	35
A4	32.2	50	20	27.5
A5	27.45	37	18	10
A6	29.7	142	64	56
A7	27.04	172	41	86.5
A8	26.18	98	31	38.5
A9	28.92	172	65	75
A10	31.3	135	57	66

>TRINITY_DN39606_c0_g1_i6 from sample A9 / Example spliced isoform

GTGGTCCGTCACTACCCTTTTTGGTATCTTCTAATTCTCAAATTTAATTATTTTGGTTTGGTTTTCCAGTGATTTATGGCTGTTTTTTTAGAAAAATAAGAATCTACATTAAGTTTGTACTGTATTGTTTAACCTCCTGTGGATGGGATCAGAGATGCATACCGTCAATATCATCGATCTGATTTATCTCAGTTGAATTT
TTATTGCTGACTGATTGGTGTGCTACTGCTACTGGTGACCTGTTCGATTTCTCTCATTGCGTCTCTGTCTTCGTTTCTGTGATAGGCAGAATAGTTTCAGATTTGTGTGGGTTACGGGGAACCCTGAAGGATGGATAGACAAATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAA
GGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTACTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGG
AGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAATCATTTTACGGATGATATAAGTAAGCGGTTGACAGGAA
AGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTTCTTTCTGCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGAAGATGGGTTAGAAGATGGGTCAGAATCCAGATCAACTGAAGACCAAATAGAGAGTGATGGGGAGCAAGATCATCATCTA
CTAATTTCTTCCAATGGGGAGGCTGAGTCAATCGAAAGGGAAAATATATGGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCCCAAGCATGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGCTGGTTCATGATCTTTTTGCTGCTCAATTTCTTCCATACTGAAGTTAGTGCATATAAAC
TACTTGGTATAGCCCTATTTCATAATAGAGCTATTACTTGGGTCAAGTTTCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTTCTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGGATTTCCAATAGGAAGTCTACCTTCACATTTTGTGGCTGTAAACAATTAGTTCCGTTAG
GTCAAATTCATCTGCAGTGTGTTTACTTTCTATGGGTTATGGAAGAAGAAATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTCTTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTGCATCATTTTGGAAATATCAATATTGTATGTCTTTTATGGGGCCAATAATTTTTCTTGT
GCAGCTATTTCCTTTTTTCCATGTGAGCGTTCATTTTTATCTAATCATTTATGAACTCTATGACTTGCTCTCTGCCTGAAACAGGCTTTGCTTTGAATTTTTATGGGCATGCCTTTGCATATCTGTGTGACAC

>TRINITY_DN39606_c0_g1_i5 from sample A9 / Example unspliced isoform:

GTGGTCCGTCACTACCCTTTTTGGTATCTTCTAATTCTCAAATTTAATTATTTTGGTTTGGTTTTCCAGTGATTTATGGCTGTTTTTTTAGAAAAATAAGAATCTACATTAAGTTTGTACTGTATTGTTTAACCTCCTGTGGATGGGATCAGAGATGCATACCGTCAATATCATCGATCTGATTTATCTCAGTTGAATTT
TTATTGCTGACTGATTGGTGTGCTACTGCTACTGGTGACCTGTTCGATTTCTCTCATTGCGTCTCTGTCTTCGTTTCTGTGATAGGCAGAATAGTTTCAGgtctctgtcaatctctggtatctgttaatctgatgtacacgcatttgcctgattatgccgctgtttggtttaggaaaatgaagaatttggttttaatatg
tcaacagtaactaaatttgggatcggttagatcaccagtttagcctttgcagATTTGTGTGGGTTACGGGGAACCCTGAAGGATGGATACACAGATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGC
AGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTACTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCG
CTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAATCATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTTCTTTCT
GCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAG

Intron 4 GTCTCTGTCA ... GATTGAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.62	32	7	21.3
A2	33.66	51.8	32	20.5
A3	31.06	74.6	19	48.3
A4	36.05	50.1	20	36.5
A5	32.79	36.9	18	18
A6	31.73	141.5	63.8	67.8
A7	28.97	172.3	41	99.5
A8	28.88	97.6	31	48.3
A9	30.23	173.1	65	85
A10	31.18	135.8	57	66

>TRINITY_DN114870_c0_g1_i3 from sample A1 / Example spliced isoform

GTTTTCCAGTGATTTTTGGCTGTTTTTTATAAAAATAAGAATCTACATTAAGTTTGTACTGTATTGTTTAACCTCCTGTGGATGGGATCGGAGATGCATACCGTCAATATCATCGATCTGATTTATCTCAGTTGAATTTTTATTGCTGACTGATTGGTGTGCTACTGCTACTGGTGACCTGTTCGATTTCTCTCATTGCG
TCTCTGTCTTCGTTTCTGTGATAGGCAGAATAGTTTCAGATTTGTGTGGGTTACGGGGAACCCTGAAGGATGGATAGACAAATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGCAGCAGCAGCCGAG
CTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTATTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCGCTCTGTTTCATTG
GGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAATCATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTTCTTTCTGCAACTGTTTGTT
GCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGTTTCTGCTGTAGAAGATGGGTTAGAAGATGGGTCAGAATCCAGATCAACTGAAGACCAAATAGAGAGTGATGGGGAGCAAGATCATCATCTACTAATTTCTTCCAATGGGGAGGCTGAGTCAATCGAAAGGGAAAATATAT
GGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCTCAAGCATGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGCTGGTTCATGATCTTTTTGCTGCTCAATTTCTTCCATACTGAAGTTAGTGCATATAAACTACTTGGTATAGCCCTATTTCATAATAGAGCTATTACTTGGGTCAAGTT
TCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTTCTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGGATTTCCAATAGGAAGTCTACTTTCACATTTTGTGGCTGTAAACAATTAGTTCCGTTAGGTCAAATTCATCTGCAGTGTGTTTACTTTCTATGGGTTATGGAAGAAGA
AATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTCTTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTGCATCATTTTGGAAATATCAATATTGTATGTCTTTTATGGGGCCAAAAAAA

>TRINITY_DN114870_c0_g1_i2 from sample A1 / Example unspliced isoform:

GTTTTCCAGTGATTTTTGGCTGTTTTTTATAAAAATAAGAATCTACATTAAGTTTGTACTGTATTGTTTAACCTCCTGTGGATGGGATCGGAGATGCATACCGTCAATATCATCGATCTGATTTATCTCAGTTGAATTTTTATTGCTGACTGATTGGTGTGCTACTGCTACTGGTGACCTGTTCGATTTCTCTCATTGCG
TCTCTGTCTTCGTTTCTGTGATAGGCAGAATAGTTTCAGgtctctgtcaatctctggtatctgttaatctgatgtacacgcatttgcctgattatgccgctgtttggtttaggaaaatgaagaatttggttttaatatgtcaacagtaactaaatttgggatcggttagatcaccagtttagcctttgcaggttactatt
tactgttatttgggttatttctctctgccagtgttgttaaggatttctctctgtggactccgattgttgtctttcttatgcttttgatttggggttgtagtgtatctgccaccagtttagctaagaatttcatattgtgggttcttcattttattccacggtttcacccgacaaattttttgtctgggattgagcagATT
TGTGTGGGTTACGGGGAACCCTGAAGGATGGATAGACAAATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACA
ACTACATGTATTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATT
GAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAATCATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTTCTTTCTGCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGA
ATATCAAGGGTTGTCAGAAGATGGGTTAGAAGATGGGTCAGAATCCAGATCAACTGAAGACCAAATAGAGAGTGATGGGGAGCAAGATCATCATCTACTAATTTCTTCCAATGGGGAGGCTGAGTCAATCGAAAGGGAAAATATATGGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCTCAAGCA
TGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGCTGGTTCATGATCTTTTTGCTGCTCAATTTCTTCCATACTGAAGTTAGTGCATATAAACTACTTGGTATAGCCCTATTTCATAATAGAGCTATTACTTGGGTCAAGTTTCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTT
CTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGGATTTCCAATAGGAAGTCTACTTTCACATTTTGTGGCTGTAAACAATTAGTTCCGTTAGGTCAAATTCATCTGCAGTGTGTTTACTTTCTATGGGTTATGGAAGAAGAAATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTC
TTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTGCATCATTTTGGAAATATCAATATTGTATGTCTTTTATGGGGCCAAAAAAA

Intron 5 GTATCTTCTA ... ATAGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	34.78	60	0	32
A2	48.15	56	1	52
A3	48.39	80	1	75
A4	43.86	64	0	50
A5	61.67	23	0	37
A6	51.64	133	7	142
A7	47.12	193	2	172
A8	42.61	132	0	98
A9	46.61	197	0	172
A10	50	135	2	135

>TRINITY_DN39606_c0_g1_i7 from sample A9 / Example spliced isoform

ATAAATATCCCTTGATCGATCTCGCAACCTCTTCATTTTATTTTAACCGCATGTGACAAAGGTAGACAGCCTGCCTTTGACATCTCGCAATCGAACTTACGTGGGAGATTGAGCGTGGTCCGTCACTACCCTTTTTGATTTGTGTGGGTTACGGGGAACCCTGAAGGATGGATACACAGATGAGCTGGAAATAACCATTC
ATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTACTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGG
AATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAAT
CATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTTCTTTCTGCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGAAGATGGGTTAGAAGATGGGTCAGAATCCAGATCAACTGAAGA
CCAAATAGAGAGTGATGGGGAGCAAGATCATCATCTACTAATTTCTTCCAATGGGGAGGCTGAGTCAATCGAAAGGGAAAATATATGGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCCCAAGCATGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGCTGGTTCATGATCTTTTTGCTG
CTCAATTTCTTCCATACTGAAGTTAGTGCATATAAACTACTTGGTATAGCCCTATTTCATAATAGAGCTATTACTTGGGTCAAGTTTCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTTCTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGGATTTCCAATAGGAAGTCTACC
TTCACATTTTGTGGCTGTAAACAATTAGTTCCGTTAGGTCAAATTCATCTGCAGTGTGTTTACTTTCTATGGGTTATGGAAGAAGAAATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTCTTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTGCATCATTTTGGAAATATCAAT
ATTGTATGTCTTTTATGGGGCCAATAATTTTTCTTGTGCAGCTATTTCCTTTTTTCCATGTGAGCGTTCATTTTTATCTAATCATTTATGAACTCTATGACTTGCTCTCTGCCTGAAACAGGCTTTGCTTTGAATTTTTATGGGCATGCCTTTGCATATCTGTGTGACAC

>TRINITY_DN39606_c0_g1_i6 from sample A9 / Example unspliced isoform:

GTGGTCCGTCACTACCCTTTTTGgtatcttctaattctcaaatttaattattttggtttggttttccagtgatttatggctgtttttttagaaaaataagaatctacattaagtttgtactgtattgtttaacctcctgtggatgggatcagagatgcataccgtcaatatcatcgatctgatttatctcagttgaattt
ttattgctgactgattggtgtgctactgctactggtgacctgttcgatttctctcattgcgtctctgtcttcgtttctgtgataggcagaatagtttcagATTTGTGTGGGTTACGGGGAACCCTGAAGGATGGATAGACAAATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAA
GGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTACTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGG
AGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAATCATTTTACGGATGATATAAGTAAGCGGTTGACAGGAA
AGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTTCTTTCTGCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGAAGATGGGTTAGAAGATGGGTCAGAATCCAGATCAACTGAAGACCAAATAGAGAGTGATGGGGAGCAAGATCATCATCTA
CTAATTTCTTCCAATGGGGAGGCTGAGTCAATCGAAAGGGAAAATATATGGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCCCAAGCATGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGCTGGTTCATGATCTTTTTGCTGCTCAATTTCTTCCATACTGAAGTTAGTGCATATAAAC
TACTTGGTATAGCCCTATTTCATAATAGAGCTATTACTTGGGTCAAGTTTCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTTCTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGGATTTCCAATAGGAAGTCTACCTTCACATTTTGTGGCTGTAAACAATTAGTTCCGTTAG
GTCAAATTCATCTGCAGTGTGTTTACTTTCTATGGGTTATGGAAGAAGAAATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTCTTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTGCATCATTTTGGAAATATCAATATTGTATGTCTTTTATGGGGCCAATAATTTTTCTTGT
GCAGCTATTTCCTTTTTTCCATGTGAGCGTTCATTTTTATCTAATCATTTATGAACTCTATGACTTGCTCTCTGCCTGAAACAGGCTTTGCTTTGAATTTTTATGGGCATGCCTTTGCATATCTGTGTGACAC

Intron 6 GTATCTTCTA ... GATTGAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.93	60	0	21
A2	27.27	56	1	21
A3	37.98	80	1	49
A4	36	64	0	36
A5	42.5	23	0	17
A6	33.5	133	7	67
A7	34.13	193	2	100
A8	27.07	132	0	49
A9	29.39	197	0	82
A10	32.84	135	2	66

>TRINITY_DN39606_c0_g1_i7 from sample A9 / Example spliced isoform

ATAAATATCCCTTGATCGATCTCGCAACCTCTTCATTTTATTTTAACCGCATGTGACAAAGGTAGACAGCCTGCCTTTGACATCTCGCAATCGAACTTACGTGGGAGATTGAGCGTGGTCCGTCACTACCCTTTTTGATTTGTGTGGGTTACGGGGAACCCTGAAGGATGGATACACAGATGAGCTGGAAATAACCATTC
ATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTACTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGG
AATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAAT
CATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTTCTTTCTGCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGAAGATGGGTTAGAAGATGGGTCAGAATCCAGATCAACTGAAGA
CCAAATAGAGAGTGATGGGGAGCAAGATCATCATCTACTAATTTCTTCCAATGGGGAGGCTGAGTCAATCGAAAGGGAAAATATATGGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCCCAAGCATGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGCTGGTTCATGATCTTTTTGCTG
CTCAATTTCTTCCATACTGAAGTTAGTGCATATAAACTACTTGGTATAGCCCTATTTCATAATAGAGCTATTACTTGGGTCAAGTTTCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTTCTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGGATTTCCAATAGGAAGTCTACC
TTCACATTTTGTGGCTGTAAACAATTAGTTCCGTTAGGTCAAATTCATCTGCAGTGTGTTTACTTTCTATGGGTTATGGAAGAAGAAATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTCTTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTGCATCATTTTGGAAATATCAAT
ATTGTATGTCTTTTATGGGGCCAATAATTTTTCTTGTGCAGCTATTTCCTTTTTTCCATGTGAGCGTTCATTTTTATCTAATCATTTATGAACTCTATGACTTGCTCTCTGCCTGAAACAGGCTTTGCTTTGAATTTTTATGGGCATGCCTTTGCATATCTGTGTGACAC

>TRINITY_DN39606_c0_g1_i4 from sample A9 / Example unspliced isoform:

GTGGTCCGTCACTACCCTTTTTGgtatcttctaattctcaaatttaattattttggtttggttttccagtgatttatggctgtttttttagaaaaataagaatctacattaagtttgtactgtattgtttaacctcctgtggatgggatcagagatgcataccgtcaatatcatcgatctgatttatctcagttgaattt
ttattgctgactgattggtgtgctactgctactggtgacctgttcgatttctctcattgcgtctctgtcttcgtttctgtgataggcagaatagtttcaggtctctgtcaatctctggtatctgttaatctgatgtacacgcatttgcctgattatgccgctgtttggtttaggaaaatgaagaatttggttttaatatg
tcaacagtaactaaatttgggatcggttagatcaccagtttagcctttgcaggttactatttactgttatttgggttatttctctctgccagtgttgttaaggatttctctctgtggactccgattgttgtctttcttatgcttttgatttggggttgtagtgtatctgccaccagtttagctaagaatttcatattgtg
ggttcttcattttattccacggtttcacccgacaaattttttgtctgggattgagcagATTTGTGTGGGTTACGGGGAACCCTGAAGGATGGATAGACAAATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGAT
TCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTACTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATT
CAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAATCATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTT
CTTTCTGCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGAAGATGGGTTAGAAGATGGGTCAGAATCCAGATCAACTGAAGACCAAATAGAGAGTGATGGGGAGCAAGATCATCATCTACTAATTTCTTCCAATGGGGAGGCTGAGTCAATCGAAAGGGAA
AATATATGGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCCCAAGCATGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGCTGGTTCATGATCTTTTTGCTGCTCAATTTCTTCCATACTGAAGTTAGTGCATATAAACTACTTGGTATAGCCCTATTTCATAATAGAGCTATTACTTGGG
TCAAGTTTCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTTCTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGGATTTCCAATAGGAAGTCTACCTTCACATTTTGTGGCTGTAAACAATTAGTTCCGTTAGGTCAAATTCATCTGCAGTGTGTTTACTTTCTATGGGTTATGG
AAGAAGAAATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTCTTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTGCATCATTTTGGAAATATCAATATTGTATGTCTTTTATGGGGCCAATAATTTTTCTTGTGCAGCTATTTCCTTTTTTCCATGTGAGCGTTCATTTTTATCT
AATCATTTATGAACTCTATGACTTGCTCTCTGCCTGAAACAGGCTTTGCTTTGAATTTTTATGGGCATGCCTTTGCATATCTGTGTGACAC

Intron 7 GTGATTCCTA ... GCAAGAATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	83.33	20	100	100
A2	82.55	13	55	68
A3	86.99	8	40	67
A4	87.5	9	71	55
A5	88.89	4	29	35
A6	83.01	52	234	274
A7	84.39	49	248	282
A8	82.7	48	198	261
A9	78.91	66	233	261
A10	84.26	31	152	180

>TRINITY_DN41654_c0_g1_i3 from sample A8 / Example spliced isoform

ATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTATTGGTTTGGCGTGGGCATCTACCATTCTG
GTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTTCTTTCTGCAAC
TGTTTGTTGCCAGAAAGTATTCAGGTTACAGCTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGA

>TRINITY_DN41654_c0_g1_i4 from sample A8 / Example unspliced isoform:

ATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTATTGGTTTGGCGTGGGCATCTACCATTCTG
GTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGgtgattcctatcat
ctcattgcaaaaaattgtaatcattttacggatgatataagtaagcggttgacaggaaagtccataccaggttgggtgaaccgacttgcaagaatagGTTCTTTCTGCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGAAGATGGGTTAGAAGATGGGTC
AGAATCCAGATCAACTGAAGACCAAATAGAGAGTGATGGGGAGCAAGATCATCATCTACTAATTTCTTCCAATGGGGAGGCTGAGTCAATCGAAAGGGAAAATATATGGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCTCAAGCATGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGC
TGGTTCATGATCTTTTTGCTGCTCAATTTCTTCCATACTGAAGTTAGTGCATATAAACTACTTGGTATAGCCCTATTTCATAATAGAGCTATTACTTGGGTCAAGTTTCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTTCTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGG
ATTTCCAATAGGAAGTCTACCTTCACATTTTGTGGCTGTAAACAATTAGTTCCGTTAGGTCAAATTCATCTGCAGTGTGTTTACTTTCTATGGGTTATGGAAGAAGAAATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTCTTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTG
CATCATTTTGGAAATATCAATATTGTATGTCTTTTATGGGGATAATAATTTTTCTTGTGCAGCT

Intron 8 GTATCTTCTA ... GCCTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.59	59.5	0	14.5
A2	17.65	56	1	12
A3	31.11	77.5	1	35
A4	29.42	66	0	27.5
A5	30.77	22.5	0	10
A6	29.63	133	7	56
A7	30.99	192.5	2	86.5
A8	22.85	130	0	38.5
A9	27.37	199	0	75
A10	33.34	132	2	66

>TRINITY_DN39606_c0_g1_i7 from sample A9 / Example spliced isoform

ATAAATATCCCTTGATCGATCTCGCAACCTCTTCATTTTATTTTAACCGCATGTGACAAAGGTAGACAGCCTGCCTTTGACATCTCGCAATCGAACTTACGTGGGAGATTGAGCGTGGTCCGTCACTACCCTTTTTGATTTGTGTGGGTTACGGGGAACCCTGAAGGATGGATACACAGATGAGCTGGAAATAACCATTC
ATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGCAGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTACTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGG
AATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCGCTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAAT
CATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTTCTTTCTGCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAGAAGATGGGTTAGAAGATGGGTCAGAATCCAGATCAACTGAAGA
CCAAATAGAGAGTGATGGGGAGCAAGATCATCATCTACTAATTTCTTCCAATGGGGAGGCTGAGTCAATCGAAAGGGAAAATATATGGGAAACACCCTTGAAGCATATCAAGGAGATCTTGTAAATTTATACCCCAAGCATGTGTTTGCAGTTCGTCAGGGAGTACAGTATATTCTTGCTGGTTCATGATCTTTTTGCTG
CTCAATTTCTTCCATACTGAAGTTAGTGCATATAAACTACTTGGTATAGCCCTATTTCATAATAGAGCTATTACTTGGGTCAAGTTTCACAGCACTGCAGCTCGTTCCATTTCTTATGAGAGCCAGGGTTATTTTTTCTTCTTATTTTCAATAAGCATTGACCTTTTTCAGTCACTTGGATTTCCAATAGGAAGTCTACC
TTCACATTTTGTGGCTGTAAACAATTAGTTCCGTTAGGTCAAATTCATCTGCAGTGTGTTTACTTTCTATGGGTTATGGAAGAAGAAATGGAGGAATAATGCACGAAGAGGTAGTGCTAAACCCCTATGTTTGATCCTTCTTTTGACATTACATTGTGCTCGGTAAGTATTGCTGCTTGCATCATTTTGGAAATATCAAT
ATTGTATGTCTTTTATGGGGCCAATAATTTTTCTTGTGCAGCTATTTCCTTTTTTCCATGTGAGCGTTCATTTTTATCTAATCATTTATGAACTCTATGACTTGCTCTCTGCCTGAAACAGGCTTTGCTTTGAATTTTTATGGGCATGCCTTTGCATATCTGTGTGACAC

>TRINITY_DN39606_c0_g1_i5 from sample A9 / Example unspliced isoform:

GTGGTCCGTCACTACCCTTTTTGgtatcttctaattctcaaatttaattattttggtttggttttccagtgatttatggctgtttttttagaaaaataagaatctacattaagtttgtactgtattgtttaacctcctgtggatgggatcagagatgcataccgtcaatatcatcgatctgatttatctcagttgaattt
ttattgctgactgattggtgtgctactgctactggtgacctgttcgatttctctcattgcgtctctgtcttcgtttctgtgataggcagaatagtttcaggtctctgtcaatctctggtatctgttaatctgatgtacacgcatttgcctgattatgccgctgtttggtttaggaaaatgaagaatttggttttaatatg
tcaacagtaactaaatttgggatcggttagatcaccagtttagcctttgcagATTTGTGTGGGTTACGGGGAACCCTGAAGGATGGATACACAGATGAGCTGGAAATAACCATTCATGTTTAGAAGAGAAAAGGAACGCAGTGCATTCTAAAGGCTGAACAATGAGACCGATTAGAATCACTTCAGATCCTGATTCCAGC
AGCAGCAGCCGAGCTCAAGTGTATTTGAATGTCTATGATCTGACCCCTTTGAACAACTACATGTACTGGTTTGGCGTGGGCATCTACCATTCTGGTATTGAAGCCCATGGTGTGGAATACGCTTTTGGAGCGCATGATTACCCTAGCAGTGGAGTATTTGAAGTGGAACCCAAGAGTTGTCCAGGCTTTGTATTCAGGCG
CTCTGTTTCATTGGGCCCTGTAGATATGTCTCCAATTGAATTCCGACAGTTCATTGAACATATGGCAGGCACTTACAATGGTGATTCCTATCATCTCATTGCAAAAAATTGTAATCATTTTACGGATGATATAAGTAAGCGGTTGACAGGAAAGTCCATACCAGGTTGGGTGAACCGACTTGCAAGAATAGGTTCTTTCT
GCAACTGTTTGTTGCCAGAAAGTATTCAGGTTACAACTGTAAGGCATACACCAGAATATCAAGGGTTGTCAG