sp|Q9FVG7|METK2_PINCO ----> S-adenosylmethionine synthase 2

Intron 1 GTAATTCATT ... GTGGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.59	7	19	29.7
A2	-1	0	0	0
A3	100	0	11	3
A4	100	0	2	10
A5	81.82	1	1	8
A6	66.67	1	2	2
A7	86.86	3	19	20.7
A8	76.19	5	10	22
A9	33.33	6	4	2
A10	79.31	3	8	15

>TRINITY_DN123399_c0_g1_i2 from sample A1 / Example spliced isoform

CCTTATCTCTCAACCTGAGACCTGAGAGGGAGGCCAAGGGTGGTTTCAGTAGAGTTGCTATTATTGATGATTTGTTTTAGGGTTTGGAGTGTTTGCTATGTCATCGTTAATTGTGATCTAGATTATAAGGGTCCGTCACCAACATCATGCCAAGGTGGCGGACTCTCAAGCAATCCATTGGCCAACGTATTAAGCCAGGA
ACATCTCCTATGAGGAGTTCAGCTCAAAGCTATTTGACTTGACTTGCACTGTTCCTCTTCCTCTTCCTCCTCCGCTTCGTACTCGCTCGTTCCAGGGAAACTCTGATCGAGATGGAGACACCCAGAC

>TRINITY_DN123399_c0_g1_i5 from sample A1 / Example unspliced isoform:

CCTTATCTCTCAACCTGAGACCTGAGAGGGAGGCCAAGGGTGGTTTCAGTAGAGTTGCTATTATTGATGATTTGTTTTAGGGTTTGGAGTGTTTGCTATGTCATCGTTAATTGTGATCTAGATTATAAGGGTCCGTCACCAACATCATGCCAAGGTGGCGGACTCTCAAGCAATCCATTGGCCAACGTATTAAGCCAGGA
ACATCTCCTATGAGGAGTTCAGCTCAAAGCTATTTGACTTGACTTGCACTGTTCCTCTTCCTCTTCCTCCTCCGCTTCGTACTCGCTCGTTCCAGGGAAACTCTGgtaattcatttgctctcagcccattttttaattttgttaaataaaggctgcaaatgtgttgaaaatttggggaattttttacgaggaaacatcta
cgtgacaagggaattaggggaagattgattgtggatgtggccttcttttaccaggttcaattatataaagttttgtttccatagatgtcttgttgcagggatgaccaattatgcgcagatcttctctttgtattgcattggtattggatcttatgggtttctgatctgggttttgtttgtttgttgttagatcttgattt
atatagatcttcttatggatttctgatctgggttttgtttgtttgttgtggttgcagATCGAGATGGAGACACCCAGACAAACTGTTTGACCATATTTTGATGCAGTGTTGGAGGCAATGCCAGCGTTGGTGCTTTGCTGATTTCAGGTATGTACTAGTTAGTCACCAGTGGTGCTATTTCCTTGAAGTGTTGTCAGCTT
TGGTGCTTTGCTGATTTCAGGTATGTACTAGTTAGTTATTTCCAAGACTTTCAGATTTATTTTTATTCACTCTGTTGTGTATGCTAGAATTGAAGTCGACATGGCTTAACAGTAACAATTATTGATAATATTAATGCATATTAAAGTATGAAAGAAGCATATAAGTTTCTAGTCCAACTCTTCATTCAAATGTGACACCT
TAGCACATCATGATCAACTGAATCCTTGGCACTAGGAAGGTCTACCAATATGTCGTATACATAAGATTCTTCTCATAGTAAACTTGCGTATCTTTATGCCAACACACGAAAAAAATAGAAACACTGTAAATGACTATAACTGTTCCAACCATCTTCATCAATGCTAGAGATGCTCTGATCCGTTTCCAATCGTTGAATTA
ATGGACTGACTATAAAACTATTGTCAAATTTTGTTTTTGTCTATTTCTGTAAGTTGGAGATTTTTATCATTTATTTCGGTAGTTTAGAGGAAAACAATATCAAATAATATGTTATATTCTTTAACCAGAGGCCACTTTACCAAGAGGCTGAGGAGATTGGAGCCAGTGACCAGGGTGACATGTATTTAATTTTGTTGTTA
TGAATATTATGCTTTGTCTTGATTTTTTTTATTTATTTAATTTTGTTCTTATTAATATTAAGTTTCATTAAGTTTCACTGATCCATTAATTTTTTTTATTTATTTAATTTTGTTCTTATTAATATTAAGTTTCATTAAGTTTCACTGATCCATTAATTTTTTTTATTTATTTAATTTT

Intron 2 GTTTTTCTTT ... GTGGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.25	49.3	15.1	107
A2	62.86	47.1	9	79.4
A3	66.91	60.9	24	112.6
A4	73.42	46.9	17	103
A5	75.48	30.3	17	68.7
A6	66.69	139.4	24.6	258.3
A7	61.98	246.7	59	397.1
A8	65.17	91.4	31	166.7
A9	64.8	195.7	38	363.1
A10	60.73	246.3	47	387.4

>TRINITY_DN123399_c0_g1_i17 from sample A1 / Example spliced isoform

AGCACTTGACCTGTGCATTGCTGCTCTTGCTCTTCCGCTTCTTAGTCGTTCATTCCAAGAAAACTCTAGTCCTCTAGGTAATTGATTTGCAGCCGATCTCAGGCTGCAAATGTGTTGAAAAATTGGGGAATTTTTTGTAAGAAGCGTCTACATGCCAGGAGAATTAGGGGAAACTTGATTGTGGAAGTATGCAGGGTTGA
ATTATTTAGAGTTTACTGTTTTATCTCCATGGATGTATCGTTGCAGGGATGACCAATTGAGTGCAGAATTGAGATGGAGACCTTCCTCTTCACATCTGAGTCTGTGAACGAGGGACACCCAGACAAGCTGTGTGACCAAATCTCTGACGCAGTGTTGGATGCATGCCTCACCCAGGACCCTGACAGCAAGGTTGCCTGCG
AGACTTGCACTAAAACGAACATGGTCATGGTTTTTGGTGAAATCACCACCAAGGCCAATGTTGATTATGAGCAGATCGTTCGCAAGACCTGCAGGGAGATTGGTTTCATTTCTGACGATGTGGGTCTTGATGCTGATCACTGCAAAGT

>TRINITY_DN123399_c0_g1_i7 from sample A1 / Example unspliced isoform:

AGCACTTGACCTGTGCATTGCTGCTCTTGCTCTTCCGCTTCTTAGTCGTTCATTCCAAGAAAACTCTAGTCCTCTAGGTAATTGATTTGCAGCCGATCTCAGGCTGCAAATGTGTTGAAAAATTGGGGAATTTTTTGTAAGAAGCGTCTACATGCCAGGAGAATTAGGGGAAACTTGATTGTGGAAGTATGCAGGGTTGA
ATTATTTAGAGTTTACTGTTTTATCTCCATGGATGTATCGTTGCAGGGATGACCAATTGAGTGCAGgtttttctttataatttgcatgggtattggatcttgatttatatagatcttcttatgggtttttgatgtgggtcttgtttgtttgtttgttgtggttgcagAATTGAGATGGAGACCTTCCTCTTCACATCTGA
GTCTGTGAACGAGGGACACCCAGACAAGCTGTGTGACCAAATCTCTGACGCAGTGTTGGATGCATGCCTCACCCAGGACCCTGACAGCAAGGTTGCCTGCGAGACTTGCACTAAAACGAACATGGTCATGGTTTTTGGTGAAATCACCACCAAGGCCAATGTTGATTATGAGCAGATCGTTCGCAAGACCTGCAGGGAGA
TTGGTTTCATTTCTGACGATGTGGGTCTTGATGCTGATCACTGCAAAGT

Intron 3 GTATGCAGGG ... TTGAGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.11	87.6	7.9	49.3
A2	43.11	72.4	13	47.1
A3	37.01	104.3	17.7	60.9
A4	27.85	100.1	21.4	46.9
A5	24.4	67	7.6	30.3
A6	39.76	227.3	39.7	139.4
A7	39.34	376.1	74	246.7
A8	37.53	155.7	28.3	91.4
A9	35.85	353	38.3	195.7
A10	38.56	393.3	72.4	246.3

>TRINITY_DN123399_c0_g1_i9 from sample A1 / Example spliced isoform

AGCACTTGACCTGTGCATTGCTGCTCTTGCTCTTCCGCTTCTTAGTCGTTCATTCCAAGAAAACTCTAGTCCTCTAGGTAATTGATTTGCAGCCGATCTCAGGCTGCAAATGTGTTGAAAAATTGGGGAATTTTTTGTAAGAAGCGTCTACATGCCAGGAGAATTAGGGGAAACTTGATTGTGGAAAATTGAGATGGAGA
CCTTCCTCTTCACATCTGAGTCTGTGAACGAGGGACACCCAGACAAGCTGTGTGACCAAATCTCTGACGCAGTGTTGGATGCATGCCTCACCCAGGACCCTGACAGCAAGGTTGCCTGCGAGACTTGCACTAAAACGAACATGGTCATGGTTTTTGGTGAAATCACCACCAAGGCCAATGTTGATTATGAGCAGATCGTT
CGCAAGACCTGCAGGGAGATTGGTTTCATTTCTGACGATGTGGGTCTTGATGCTGATCACTGCAAAGT

>TRINITY_DN123399_c0_g1_i17 from sample A1 / Example unspliced isoform:

AGCACTTGACCTGTGCATTGCTGCTCTTGCTCTTCCGCTTCTTAGTCGTTCATTCCAAGAAAACTCTAGTCCTCTAGGTAATTGATTTGCAGCCGATCTCAGGCTGCAAATGTGTTGAAAAATTGGGGAATTTTTTGTAAGAAGCGTCTACATGCCAGGAGAATTAGGGGAAACTTGATTGTGGAAgtatgcagggttga
attatttagagtttactgttttatctccatggatgtatcgttgcagggatgaccaattgagtgcagAATTGAGATGGAGACCTTCCTCTTCACATCTGAGTCTGTGAACGAGGGACACCCAGACAAGCTGTGTGACCAAATCTCTGACGCAGTGTTGGATGCATGCCTCACCCAGGACCCTGACAGCAAGGTTGCCTGCG
AGACTTGCACTAAAACGAACATGGTCATGGTTTTTGGTGAAATCACCACCAAGGCCAATGTTGATTATGAGCAGATCGTTCGCAAGACCTGCAGGGAGATTGGTTTCATTTCTGACGATGTGGGTCTTGATGCTGATCACTGCAAAGT

Intron 4 GTATGCAGGG ... GTGGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.39	73.4	8.9	91.9
A2	59.79	59.9	9	67
A3	55.43	86.6	22.4	94.6
A4	52.09	85.3	22.7	88.9
A5	49.59	56.9	10.7	58.3
A6	59.69	187.4	27.4	217.1
A7	51.83	318.3	57.3	338.3
A8	53.94	130	27.4	141.1
A9	50.81	293.4	37.3	302.7
A10	48.98	332.6	67.9	327.7

>TRINITY_DN123399_c0_g1_i9 from sample A1 / Example spliced isoform

AGCACTTGACCTGTGCATTGCTGCTCTTGCTCTTCCGCTTCTTAGTCGTTCATTCCAAGAAAACTCTAGTCCTCTAGGTAATTGATTTGCAGCCGATCTCAGGCTGCAAATGTGTTGAAAAATTGGGGAATTTTTTGTAAGAAGCGTCTACATGCCAGGAGAATTAGGGGAAACTTGATTGTGGAAAATTGAGATGGAGA
CCTTCCTCTTCACATCTGAGTCTGTGAACGAGGGACACCCAGACAAGCTGTGTGACCAAATCTCTGACGCAGTGTTGGATGCATGCCTCACCCAGGACCCTGACAGCAAGGTTGCCTGCGAGACTTGCACTAAAACGAACATGGTCATGGTTTTTGGTGAAATCACCACCAAGGCCAATGTTGATTATGAGCAGATCGTT
CGCAAGACCTGCAGGGAGATTGGTTTCATTTCTGACGATGTGGGTCTTGATGCTGATCACTGCAAAGT

>TRINITY_DN123399_c0_g1_i7 from sample A1 / Example unspliced isoform:

AGCACTTGACCTGTGCATTGCTGCTCTTGCTCTTCCGCTTCTTAGTCGTTCATTCCAAGAAAACTCTAGTCCTCTAGGTAATTGATTTGCAGCCGATCTCAGGCTGCAAATGTGTTGAAAAATTGGGGAATTTTTTGTAAGAAGCGTCTACATGCCAGGAGAATTAGGGGAAACTTGATTGTGGAAgtatgcagggttga
attatttagagtttactgttttatctccatggatgtatcgttgcagggatgaccaattgagtgcaggtttttctttataatttgcatgggtattggatcttgatttatatagatcttcttatgggtttttgatgtgggtcttgtttgtttgtttgttgtggttgcagAATTGAGATGGAGACCTTCCTCTTCACATCTGA
GTCTGTGAACGAGGGACACCCAGACAAGCTGTGTGACCAAATCTCTGACGCAGTGTTGGATGCATGCCTCACCCAGGACCCTGACAGCAAGGTTGCCTGCGAGACTTGCACTAAAACGAACATGGTCATGGTTTTTGGTGAAATCACCACCAAGGCCAATGTTGATTATGAGCAGATCGTTCGCAAGACCTGCAGGGAGA
TTGGTTTCATTTCTGACGATGTGGGTCTTGATGCTGATCACTGCAAAGT