NA ----> NA

Intron 1 GTAAGACTTA ... TTTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.33	4	12	10
A2	86.67	1	9	4
A3	60	2	0	6
A4	100	0	5	1
A5	42.86	2	0	3
A6	48.94	12	18	5
A7	79.59	10	59	19
A8	68	20	54	31
A9	70	6	19	9
A10	78.38	8	37	21

>TRINITY_DN43735_c0_g3_i3 from sample A6 / Example spliced isoform

AGAATATGTGATTGTTATGAATTAAATTACATAAAATCTTAAGGATCGCTACCGAGAGTAAAGCTTCCACAAGAGGATAATCTTGAGTTGTTTTGCCATTTGTGCATAACTATAATCACAGCATTTAAAAATGGTTGTTGAATGATGATATACACAGAACAGCAGAGCTGAAATCGCCAGGGATAAGGAGATGAAATAGT
GTTCAAGGACCATCGTTTGCAGGGATGCCAAGCCGTGGAGGTTACGATATCACCGAAGATAATGTGATCGAACTGTTGTTGTGCGATCATTTTGAAGTTTGACGGCGAAAGCTTTATCTGCCAGAGATATAGATCATCTATGTTATTTAAGCAGTATGAATTGGACTGTTCGAGGGGTGGCGGTCTCTGCAGACCAGAGA
AGTTGTAATCTTTCTGCTAAATTCCTTTTCTGTGGAGAAATCGATGGACTTGAGGAAACGTTCGTTTCTGTAAACTCTGTACCGTTTCTCTGCTAGGAAGCCCTCGACAGAGCTGGACAGATTCTTCTTTTAGACTGAGGAGGCGCTGTGAGGGAGATGGACTTTAGAGCTCGAATCAGCAAGGAAGCTGTTGGACACAC
CCGGTTTTTGCAGACACTGTAATTTCTTACAGTTGTGTTAATACAGTAAAGTTTCTCCATATGACTTTCTTGTTCACTCTTCAAGTTTCTTTCATCAGTTTGATGATTGCTCTGAGTCTATTAATTTGTAAACAATTAACATTCATAGATCTGAGATTTTGGTTTATCACTATTGATGATCTGAATGTGTTGAATGAGTT
TCTTTATGC

>TRINITY_DN43735_c0_g3_i8 from sample A6 / Example unspliced isoform:

AGAATATGTGATTGTTATGAATTAAATTACATAAAATCTTAAGGATCGCTACCGAGAGTAAAGCTTCCACAAGAGGATAATCTTGAGTTGTTTTGCCATTTGTGCATAACTATAATCACAGCATTTAAAAATGGTTGTTGAATGATGATATACACAGAACAGCAGAGCTGAAATCGCCAGGGATAAGGAGATGAAATAGT
GTTCAAGGACCATCgtaagacttaaccatcgagaatttaatttaatcgctcaatcagattttcgcctcaaagatcgagaagaagagttcacctactgttttacattgtggttaaagtgttttactcgaatttatataagaaggagatggtattctgcatacagaaaagggggaattttctatcttttcattatttacagt
ttacgttgctgaaattttaataacattctaccttggtaaatgaacgttgattaatattttttttttttcagGTTTGCAGGGATGCCAAGCCGTGGAGGTTACGATATCACCGAAGATAATGTGATCGAACTGTTGTTGTGCGATCATTTTGAAGTTTGACGGCGAAAGCTTTATCTGCCAGAGATATAGATCATCTATGT
TATTTAAGCAGTATGAATTGGACTGTTCGAGGGGTGGCGGTCTCTGCAGACCAGAGAAGTTGTAATCTTTCTGCTAAATTCCTTTTCTGTGGAGAAATCGATGGACTTGAGGAAACGTTCGTTTCTGTAAACTCTGTACCGTTTCTCTGCTAGGAAGCCCTCGACAGAGCTGGACAGATTCTTCTTTTAGACTGAGGAGG
CGCTGTGAGGGAGATGGACTTTAGAGCTCGAATCAGCAAGGAAGCTGTTGGACACACCCGGTTTTTGCAGACACTGTAATTTCTTACAGTTGTGTTAATACAGTAAAGTTTCTCCATATGACTTTCTTGTTCACTCTTCAAGTTTCTTTCATCAGTTTGATGATTGCTCTGAGTCTATTAATTTGTAAACAATTAACATT
CATAGATCTGAGATTTTGGTTTATCACTATTGATGATCTGAATGTGTTGAATGAGTTTCTTTATGC

Intron 2 GTAATCAATT ... TGAATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76.27	7	30	15
A2	66.67	7	14	14
A3	58.9	15	32	11
A4	51.85	13	21	7
A5	45.45	3	3	2
A6	71.88	18	53	39
A7	63.31	122	215	206
A8	67.56	103.7	199	233
A9	65.06	69	132	125
A10	62.37	105	196	152

>TRINITY_DN107787_c0_g1_i2 from sample A1 / Example spliced isoform

TGCCCACAGAGGAAGTATCGCGGGAGATTACCACGTTGCCAAATACGCTTTGGTTTATTATTTTGTTTTCTCATTACTGTTCTGTTTTGCAGATTCCACATTATCCCGCCAGCATGAAAGAAGGATTATAGCATGTAGATCCGAAGCGAAGACGCCGAGCCAGAAAACGAAACAACAAGAAGCACATTGTTTTGCTCTAT
CTGTCACAAGATCATCATTTCAGTTCGCAGAGATTCTCGGTGGCGACTGGCGATATTATTTGCGGACTACCTGTTGAGGTGATTTGAGGGCGATATTGTGTAGCAAAAGGAATTCGAGATTACTGCACCACTTATGGGATATTCATTGCCATAACGTTAAAAGAAGTGTATCGCGTTGAGAAAAGTTCGCACCAAATATT
TAAATCTGTTGCTACGAATCAAATGGAAGAAGTTACGCGGGAGGTCTTTTGTTATTATGCCATTTGTCGTCAACAAATACCAGGAAGATATTACATGCTATCGAGAATTTCAAGCAGAAAATCAAGAAGAGCACGATCATGCTTGTCAAGAAGATCGAAGATGGTTCAAAACACCAAAAAGAAAAGAGGCATTCAAGATC
GATGATTCTTTCCAAGCAAAAGTTGTACAGGAGAACCAAGACTGGGTCAGGAAAATGAGCAAGTTTTCCAAAATCAACAGTGGACGAAGATTGTAAATCCGATCTCATGCTGATTGGGGGCCAGCTGGAGGATTAAGATTAGATCGACGGGGGTTATGTGTGCTTATCTCCATATGACGAGGAAGGCTATCCCATGATTC
TAGTTAAGTCGACCACTGTCAATTACATGGAAAACTGAGAGTCTTGCAGCTCTTATAAAAGCAAGCAGAAGTCGACAGCTGAAGGGGG

>TRINITY_DN107787_c0_g1_i1 from sample A1 / Example unspliced isoform:

TGCCCACAGAGGAAGTATCGCGGGAGATTACCACGTTGCCAAATACGCTTTGGTTTATTATTTTGTTTTCTCATTACTGTTCTGTTTTGCAGATTCCACATTATCCCGCCAGCATGAAAGAAGGATTATAGCATGTAGATCCGAAGCGAAGACGCCGAGCCAGAAAACGAAACAACAAGAAGCACATTGTTTTGCTCTAT
CTGTCACAAGATCATCATTTCAGTTCGCAGAGATTCTCGGTGGCGACTGGCGATATTATTTGCGGACTACCTGTTGAGGTGATTTGAGGGCGATATTGTGTAGCAAAAGGAATTCGAGATTACTGCACCACTTATGGGATATTCATTGCCATAACGTTAAAAGAAGTGTATCGCGTTGAGAAAAGTTCGCACCAAATATT
TAAATCTGTTGCTACGAATCAAATGGAAGAAGTTACGCGGGAGGTCTTTTGTTATTATGCCATTTGTCGTCAACAAATACCAGGAAGATATTACATGCTATCGgtaatcaattgaccatgtccatattccactcctggagagagttactctaccaaacttggagaaggttatcagaacaactagaggagactatcgttgc
gacccgatggaggagaaaatgggatgccacccgcaaaaagggcttttgattgttgaattttggtttgaattttgattttgattgaatttctcttatttttattttgtgaattgcagAGAATTTCAAGCAGAAAATCAAGAAGAGCACGATCATGCTTGTCAAGAAGATCGAAGATGGTTCAAAACACCAAAAAGAAAAGA
GGCATTCAAGATCGATGATTCTTTCCAAGCAAAAGTTGTACAGGAGAACCAAGACTGGGTCAGGAAAATGAGCAAGTTTTCCAAAATCAACAGTGGACGAAGATTGTAAATCCGATCTCATGCTGATTGGGGGCCAGCTGGAGGATTAAGATTAGATCGACGGGGGTTATGTGTGCTTATCTCCATATGACGAGGAAGGC
TATCCCATGATTCTAGTTAAGTCGACCACTGTCAATTACATGGAAAACTGAGAGTCTTGCAGCTCTTATAAAAGCAAGCAGAAGTCGACAGCTGAAGGGGG

Intron 3 GTAAGACTTA ... TTTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	4	12	4
A2	81.82	1	9	0
A3	60	2	0	6
A4	100	0	5	0
A5	33.33	2	0	2
A6	51.02	12	18	7
A7	80.2	10	59	22
A8	66.39	20	54	25
A9	70	6	19	9
A10	76.47	8	37	15

>TRINITY_DN43735_c0_g3_i7 from sample A6 / Example spliced isoform

AGAATATGTGATTGTTATGAATTAAATTACATAAAATCTTAAGGATCGCTACCGAGAGTAAAGCTTCCACAAGAGGATAATCTTGAGTTGTTTTGCCATTTGTGCATAACTATAATCACAGCATTTAAAAATGGTTGTTGAATGATGATATACACAGAACAGCAGAGCTGAAATCGCCAGGGATAAGGAGATGAAATAGT
GTTCAAGGACCATCGTTTGCAGGGATGCCAAGCCGTGGAGGTTACGATATCACCGAAGATAATGTGATCGAACTGTTGTTGTGCGATCATTTTGAAGTTTGACGGCGAAAGCTTTATCTGCCAGAGATATAGATCATCTATGTTATTTAAGCAGTATGAATTGGACTGTTCAGAGGGGGGTGGGCGGTCTCTGCAGACCA
GAGAAATTGTAATTTTTGCTAATTCCTTTTTCTGTACATTTATGCTTGGAAAAGGGAGACTGAGGAGACGCTGCTGCCAAGGGGGAAAATCGATGGAGTTGAGGAAACTTTCGTTTCTGTAAACTCTGTACCATTTCTTTGCTAGGGGAGCGCTCAACAGAGCTGGATAGATTTTTCTTTCAGGACTGAGGAGGCGCTGC
CAAGGAGGAATCGAGGCAAGGGAATCAACATAGAAGAACTAAGGAAGCGCTGTTTCAATCCATGCATTGTAATTTTTTGCAACTTGATGAATACAAGCCG

>TRINITY_DN43735_c0_g3_i10 from sample A6 / Example unspliced isoform:

AGAATATGTGATTGTTATGAATTAAATTACATAAAATCTTAAGGATCGCTACCGAGAGTAAAGCTTCCACAAGAGGATAATCTTGAGTTGTTTTGCCATTTGTGCATAACTATAATCACAACATTTAAAAACGGTTGTTGAATGATGATATACACAGAACAGCAGAGCTGAAATCGCCAGGGATAAGGAGATGAAATAGT
GTTCAAGGACCATCgtaagacttaaccatcggggatttcatttaatcgctcaatcagattttcgcctcaaagatcgagaagaagagttcacctcctgttttacatcgtggttaaagtgttttactcgaatttatataagaaggagatggtattctacatacaaaaaaggggaattttctatcttttcattatttacagtt
tacgttgctggtaaatgaacgttgattaatatattttttttgcagGTTTGCAGGGATGCCAAGCCGTGGAGGTTACGATATCACCGAAGATAATGTGATCGAACTGTTGTTGTGCGATCATTTTGAAGTTTGACGGCGAAAGCTTTATCTGCCAGAGATATAGATCATCTATGTTATTTAAGCAGTATGAATTGGACTGT
TCAGAGGGGGGTGGGCGGTCTCTGCAGACCAGAGAAATTGTAATTTTTGCTAATTCCTTTTTCTGTACATTTATGCTTGGAAAAGGGAGACTGAGGAGACGCTGCTGCCAAGGGGGAAAATCGATGGAGTTGAGGAAACTTTCGTTTCTGTAAACTCTGTACCATTTCTTTGCTAGGGGAGCGCTCAACAGAGCTGGATA
GATTTTTCTTTCAGGACTGAGGAGGCGCTGCCAAGGAGGAATCGAGGCAAGGGAATCAACATAGAAGAACTAAGGAAGCGCTGTTTCAATCCATGCATTGTATAAGGTGACTTCAGAGAAAGGGTCTTCAAGAATTGCATTTAAAGGAACCTATCAAAGTCAAATTGATTAATAATGTGCACAACAGACCAAGGTGAAGG
TGTAAACCCATGAAGTCTCTAATCTAGGGATGAAGTGGTCAACTAGGCTTTAGGAGACTTCGACATGTAAAAGAAAATTCAACGTTGATAAATGTTAAAGCATGACTCCGTGATCCTTGCGAGGCATTCTCCATGGAGGTGATTAATGTTGGAAAGTGATAAATTAAGCTTTGGTAAGATATGTGTAAAGTTGTCCTACC
TGAACTATAAGGATAGAGGGTTATTTGTAAACATCCGACGTTGTATTAATATGTGTCCAAGATTATGTATGAAACTATGCAGAAGTGG

Intron 4 GTGAGTGATC ... TGTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.5	6	0	10
A2	63.64	4	7	7
A3	66.67	2	0	4
A4	50	2	0	2
A5	50	2	0	2
A6	85.71	2	0	12
A7	67.47	27	9	56
A8	55.29	38	7	47
A9	58.33	40	11	56
A10	69.33	46	5	104

>TRINITY_DN43320_c1_g2_i5 from sample A9 / Example spliced isoform

AAGGCCCTGAACGTTGGCATAGATGGACGGCGTAGATCACAATGAAAGGTTATTGTACTTCAATCAAATTCCATACAGAGATGCTCCTAAATCAGCGAAGGAAATCATCATGCAATGGTGTCATCAATTGAATGAAGAGAATAGATCACCAATAATGGAGTATGTTTTTCTCTCACCTTGTTCCATGAAAGCATGTCCGA
AGAAAGAACTCAAGATCATCACTAAGATGGAAGACGATTGTTCACATTAATCGCAAAACTACTATCACGCCAACGAATGAGGAAAATTGCACCATATTTTCGACAGTGGGTAGCTGAAGCACCGTATTTTTATTAGATTCCACATTATCCCGCCAGCATGAAAGAAGGATTATAGCATGTAGATCCGAAGCGAAGACGCC
GAGCCAGAAAACGAAACAACAAGAAGCACATTGTTTTGCTCTATCTGTCACAAGATCATCATTTCAGTTCGCAGAGATTCTCGGTGGCGACTGGCGATATTATTTGCGGACTACCTGTTGAGGTGATTTGAGGGCGATATTGTGTAGCAAAAGGAATTCGAGATTACTGCACCACTTATGGGATATTCATTGCCATAACG
TTAAAAGAAGTGTATCGCGTTGAGAAAAGTTCGCACCAAATATTTAAATCTGTTGCTACGAATCAAATGGAAGAAGTTACGCGGGAGGTCTTTTGTTATTATGCCATTTGTCGTCAACAAATACCAGGAAGATATTACATGCTATCGAGAATTTCAAGCAGAAAATCAAGAAGAGCACGATCATGCTTATCAAGAAGATC
GAAGATGGTTCAAAACACCAAAAAGAAAAGAGGCATTCAAGATCGATGATTCTTTCCAAGCAAAAGTTGTACAGGAGAACCAAGACTGGGTCAGGAAAATGAGCAAGTTTTCCAAAATCAACAGTGGACGAAGATTGTAAATCCGATCTCATGCTGATTGGGGGCCAGCTGGAGGATTAAGATTAGATCGACGGGGGTTA
TGTGTGCTTATCTCCATATGACGAGGAAGGCTATCCCATGATTCTAGTTAAGTCGACCACTGTCAATTACATGGAAAACTGAGAGTCTTGCAGCTCTTATAAAAGCAAGCAGAAGTCGACAGCTGAAGGGGGGGGGGCATTGGATGGAGCTGAGGAAACTTTAGTTTCTGTAAACTCTGTACCGTTTCTTTGCTGGGGGG
CGGTCTCTGCAGACCAGAGAAATTGTAATTTTTCTGTTAATTCCTTTTTCTGTACATTTATGCTTGGAAAAGGGAGACTGATGAGACGCTGCTGCCATGGAGATAGAATTTGGAGTCTGAAGTCAGCAGGAGAAGAGACTGGGGATACTCTGTTGCCAGAGTCATTGTAATTTCAGTACAAGACATCATAATACAGTAAT
ATTATCTTAGTATCTATCTTTTGGTTGTA

>TRINITY_DN43320_c1_g2_i4 from sample A9 / Example unspliced isoform:

AAGGCCCTGAACGTTGGCATAGATGGACGGCGTAGATCACAATGAAAGGTTATTGTACTTCAATCAAATTCCATACAGAGATGCTCCTAAATCAGCGAAGGAAATCATCATGCAATGGTGTCATCAATTGAATGAAGAGAATAGATCACCAATAATGGAGTATGTTTTTCTCTCACCTTGTTCCATGAAAGCATGTCCGA
AGAAAGAACTCAAGATCATCACTAAGATGGAAGACGATTGTTCACATTAATCGCAAAACTACTATCACGCCAACGAATGAGGAAAATTGCACCATATTTTCGACAGTGGGTAGCTGAAGCACCGTATTTTTATTAGgtgagtgatcataaaacaaaagggaattgcaatatatcacaatgtttccggccagagaatatta
ttggcatattgaacgcatagtacttctaccgaataaccaagaacatgcaaaagtcataactctctaaaaccagctgagaattgccacatggacatagtagttatgacgccatgaaagtatgggacaaatacgcaagaaaaatcagtcggttaaagacagatgcccacagaggaagtatcgcgggagattaccacgttgcc
aaatacgctttggtttattattttgttttctcattactgttctgttttgcagATTCCACATTATCCCGCCAGCATGAAAGAAGGATTATAGCATGTAGATCCGAAGCGAAGACGCCGAGCCAGAAAACGAAACAACAAGAAGCACATTGTTTTGCTCTATCTGTCACAAGATCATCATTTCAGTTCGCAGAGATTCTCGG
TGGCGACTGGCGATATTATTTGCGGACTACCTGTTGAGGTGATTTGAGGGCGATATTGTGTAGCAAAAGGAATTCGAGATTACTGCACCACTTATGGGATATTCATTGCCATAACGTTAAAAGAAGTGTATCGCGTTGAGAAAAGTTCGCACCAAATATTTAAATCTGTTGCTACGAATCAAATGGAAGAAGTTACGCGG
GAGGTCTTTTGTTATTATGCCATTTGTCGTCAACAAATACCAGGAAGATATTACATGCTATCGGTAATCAATTGACCATGTCCATATTCCACTCCTGGAGAGAGTTACTCTACCAAACTTGGAGAAGGTTATCAGAACAACTAGAGGAGACTATCGTTGCGACCCGATGGAGGAGAAAATGGGATGCCACCCGCAAAAAG
GGCTTTTGATTGTTGAATTTTGGTTTGAATTTTGATTTTGATTAAATTTCTCTTATTTTTATTTTGTGAATTGCAGAGAATTTCAAGCAGAAAATCAAGAAGAGCACGATCATGCTTATCAAGAAGATCGAAGATGGTTCAAAACACCAAAAAGAAAAGAGGCATTCAAGATCGATGATTCTTTCCAAGCAAAAGTTGTA
CAGGAGAACCAAGACTGGGTCAGGAAAATGAGCAAGTTTTCCAAAATCAACAGTGGACGAAGATTGTAAATCCGATCTCATGCTGATTGGGGGCCAGCTGGAGGATTAAGATTAGATCGACGGGGGTTATGTGTGCTTATCTCCATATGACGAGGAAGGCTATCCCATGATTCTAGTTAAGTCGACCACTGTCAATTACA
TGGAAAACTGAGAGTCTTGCAGCTCTTATAAAAGCAAGCAGAAGTCGACAGCTGAAGGGGGGGGGGCATTGGATGGAGCTGAGGAAACTTTAGTTTCTGTAAACTCTGTACCGTTTCTTTGCTGGGGGGCGGTCTCTGCAGACCAGAGAAATTGTAATTTTTCTGTTAATTCCTTTTTCTGTACATTTATGCTTGGAAAA
GGGAGACTGATGAGACGCTGCTGCCATGGAGATAGAATTTGGAGTCTGAAGTCAGCAGGAGAAGAGACTGGGGATACTCTGTTGCCAGAGTCATTGTAATTTCAGTACAAGACATCATAATACAGTAATATTATCTTAGTATCTATCTTTTGGTTGTA