NA ----> NA

Intron 1 ATATCCTAAT ... TGTATTGCAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.64	27.5	8	3
A2	32.09	15	7	0
A3	23.71	26	8	0
A4	15.42	27.5	5	2
A5	33.64	14	7	0
A6	18.59	118.5	27	0
A7	19.94	97.5	24	1
A8	7.66	97.5	8	0
A9	13.03	121	18	0
A10	22.9	64	19	0

>TRINITY_DN117301_c0_g1_i1 from sample A1 / Example spliced isoform

TATTGATGAAAATTGTACAATGAGGGCAGCCACATTTCAATGGAATGGAGAAGGTGCATTTTTGTTCACATCAAGTAGTGCCGTTTATGCTTGCAATGATAATGGACTGTGCCTTGAGGAATCCCCTACAGTGCCAATTGGGAGTAGTTTTCGGACAGATGTTCTTTTGAAAGCAGAAAACGAAGTGCTGAAAATTGGTG
GTAGTGTGATACGATTATCAGGGCTTTACAATTGGGATAGAGGTGCTCATCTATATTGCCTAGAGAAGGGGACTGTTGAAGCACGCCCAGATCATATTCTCAATCTTATCCATTATGAGGAAAGATTTAATGGATTAT

>TRINITY_DN117301_c0_g1_i3 from sample A1 / Example unspliced isoform:

ATTATAGTGTTGCTTTCACGGCGGAATTCCCTCTCGTGTAATTATCATATAAACTGTCTGCTAAAATGGCGAAGCCCGTCGGGCTGTTTTCATGGATTTCTACCGCCCAATTCCTACCACATTGCTGGGCAAATCAGTTTACAGATACCCAATAGATATTCAATGCAGGAATATCCGGCTGCTTAACCTGCCTCATCATT
CCAGCAAGTTTTACAGGAAATTGCAGGCTTGGAGCAAAAGTTTTGAGAATAATTTTATTGCATATACGAGAATGTCTTCGCCAATTTCAGCAGCTTCCACGTCTGGCATGGAAACTGTAGGAGGAGTAGAGGTTCCAGATTCACAGTCTGTTGGCGAAAAAGATTTGCTGATTGTAGGACCAGGTGTACTAGGCCGACTG
GTAGCAGAAAGCTGGCTAAAGGAAAATCCTTCTTGCAGAGTTGTTGGACAAACAAGGTCGAAGGATCATCATGAAGAGTTACAGCAACTTGGAATCAAACCATGTTTGAAGGGATCGCAAGCACATGAGCTGTTTCCTTTTGTTATTTACTGCGCTCCCCCCTCTGGAAGTGGTGAATACCCAGATGATGTCAGGGCAGC
CACATTTCAATGGAATGGAGAAGGTGCATTTTTGTTCACATCAAGTAGTGCCGTTTATGCTTGCAATGATAATGGACTGTGCCTTGAGGAATCCCCTACAGTGCCAATTGGGAGTAGTTTTCGGACAGATGTTCTTTTGAAAGCAGAAAACGAAGTGCTGAAAATTGGTGGTAGTGTGATACGATTATCAGGGCTTTACa
tatcctaataaaagaatgctgtttatccatttaaagtgtttggggaaaagtaattttctttatctatttggattttcttgactgtattgcacAATTGGGATAGAGGTGCTCATCTATATTGCCTAGAGAAGGGGACTGTTGAAGCACGCCCAGATCATATTCTCAATCTTATCCATTATGAGGA

Intron 2 GCATGGAAAC ... CTGGCTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.73	13	19	18
A2	69.23	6	9	18
A3	71.15	10.5	27	24.8
A4	70	3	8	6
A5	81.82	3	9	18
A6	65.45	33	36	89
A7	53	70.5	57	102
A8	57.15	28.5	28	48
A9	60	32	29	67
A10	69.37	17	35	42

>TRINITY_DN108080_c0_g1_i5 from sample A2 / Example spliced isoform

ATTATAGTGTTGCTTTCACGGCGGAATTCCCTCTCGTGTAATTATCATATAAACTGTCTGCTAAAATGGCGAAGCCCGTCGGGCTGTTTTCATGGATTTCTACCGCCCAATTCCTACCACATTGCTGGGCAAATCAGTTTACAGATACCCAATAGATATTCAATGCAGGAATATCCGGCTGCTTAACCTGCCTCATCATT
CCAGCAAGTTTTACAGGAAATTGCAGGCTTGGAGCAAAAGTTTTGAGAATAATTTTATTGCATATACGAGAATGTCTTCGCCAATTTCAGCAGCTTCCACGTCTGGAAAATCCTTCTTGCAGAGTTGTTGGACAAACAAGGTCGAAGGATCATCATGAAGAGTTACAGCAACTTGGAATCAAACCATGTTTGAAGGGATC
GCAAGCACATGAGCTGTTTCCTTTTGTTATTTACTGCGCTCCCCCCTCTGGAAGTGGTGAATACCCAGATGATGTCAGGGCAGCCACATTTCAATGGAATGGAGAAGGTGCATTTTTGTTCACATCAAGTAGTGCCGTTTATGCTTGCATTTTTGTTCACATCAAGTAGTGCCGTTTATGCTTGCAATGATAATGGACTG
TGCCTTGAGGAATCCCCTACAGTGCCAATTGGGAGTAGTTTTCGGACAGATGTTCTTTTGAAAGCAGAAAACGAAGTGCTGAAAATTGGTGGTAGTGTGATACGATTATCAGGGCTTTACAATTGGGATAGAGGTGCTCATCTATATTGCCTAGAGAAGGGAACTGTTGAAGCACGCCCAGATCATATTCTCAATCTTAT
CCATTATGAGGATGCAGCTTCTTTATGCATATCTATATTGAAAGGAAAGTTCAGACGGCGTGTCTTTATGGGTTGTGACAATCATCCTTTATCCAGGAAAGATTTAATGGATTATGTGAACAAAAGTGGCAAGTTTGATAAAAAGTTTCAGGGTTTCACAGGTGTTGATGGGCCCTTGGGAAAACGGATGGATAACACAA
AAACTTGTAAGGAAATTGGTTGGCAACCAAAGTATCCAAGCTTTGCCCAATTTCTTGGAATTACAGAGTGAATGCTAAACACATTGTTATGAGCATTTGCAATTTGTTGGTGCGTTTCAAGAGGGCAGTATATCTGCAACTTTCACTTTACCTAATAATATCAATGTTATTCCCCCACGGGGTCCCATTCTTAATCAATG
TAACATAAATACCTCACCAAAGACCCATTTCGGTCTTTTGGTGTGTACTTACAAGTACAAAGATTAAGGCTATATTTTTGTGCCTATAACATCCCTTGCAATGCATGGGACTGCTTTTGTTGGCACCTAAACAATTTCTTTACATTACTTATTGAATGCAGATAGATATCTCATGTCTCAATCTCGTCTGATAACACTGT
TTTTAGGAGTGAGCTGCCAAGTAATCTCGAGGTTCCCCAAGCC

>TRINITY_DN108080_c0_g1_i3 from sample A2 / Example unspliced isoform:

ATTATAGTGTTGCTTTCACGGCGGAATTCCCTCTCGTGTAATTATCATATAAACTGTCTGCTAAAATGGCGAAGCCCGTCGGGCTGTTTTCATGGATTTCTACCGCCCAATTCCTACCACATTGCTGGGCAAATCAGTTTACAGATACCCAATAGATATTCAATGCAGGAATATCCGGCTGCTTAACCTGCCTCATCATT
CCAGCAAGTTTTACAGGAAATTGCAGGCTTGGAGCAAAAGTTTTGAGAATAATTTTATTGCATATACGAGAATGTCTTCGCCAATTTCAGCAGCTTCCACGTCTGgcatggaaactgtaggaggagtagaggttccagattcacagtctgttggcgaaaaagatttgctgattgtaggaccaggtgtactaggccgactg
gtagcagaaagctggctaaagGAAAATCCTTCTTGCAGAGTTGTTGGACAAACAAGGTCGAAGGATCATCATGAAGAGTTACAGCAACTTGGAATCAAACCATGTTTGAAGGGATCGCAAGCACATGAGCTGTTTCCTTTTGTTATTTACTGCGCTCCCCCCTCTGGAAGTGGTGAATACCCAGATGATGTCAGGGCAGC
CACATTTCAATGGAATGGAGAAGGTGCATTTTTGTTCACATCAAGTAGTGCCGTTTATGCTTGCATTTTTGTTCACATCAAGTAGTGCCGTTTATGCTTGCAATGATAATGGACTGTGCCTTGAGGAATCCCCTACAGTGCCAATTGGGAGTAGTTTTCGGACAGATGTTCTTTTGAAAGCAGAAAACGAAGTGCTGAAA
ATTGGTGGTAGTGTGATACGATTATCAGGGCTTTACAATTGGGATAGAGGTGCTCATCTATATTGCCTAGAGAAGGGAACTGTTGAAGCACGCCCAGATCATATTCTCAATCTTATCCATTATGAGGATGCAGCTTCTTTATGCATATCTATATTGAAAGGAAAGTTCAGACGGCGTGTCTTTATGGGTTGTGACAATCA
TCCTTTATCCAGGAAAGATTTAATGGATTATGTGAACAAAAGTGGCAAGTTTGATAAAAAGTTTCAGGGTTTCACAGGTGTTGATGGGCCCTTGGGAAAACGGATGGATAACACAAAAACTTGTAAGGAAATTGGTTGGCAACCAAAGTATCCAAGCTTTGCCCAATTTCTTGGAATTACAGAGTGAATGCTAAACACAT
TGTTATGAGCATTTGCAATTTGTTGGTGCGTTTCAAGAGGGCAGTATATCTGCAACTTTCACTTTACCTAATAATATCAATGTTATTCCCCCACGGGGTCCCATTCTTAATCAATGTAACATAAATACCTCACCAAAGACCCATTTCGGTCTTTTGGTGTGTACTTACAAGTACAAAGATTAAGGCTATATTTTTGTGCC
TATAACATCCCTTGCAATGCATGGGACTGCTTTTGTTGGCACCTAAACAATTTCTTTACATTACTTATTGAATGCAGATAGATATCTCATGTCTCAATCTCGTCTGATAACACTGTTTTTAGGAGTGAGCTGCCAAGTAATCTCGAGGTTCCCCAAGCC

Intron 3 GTGAATACCC ... ATATTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.69	29	19	26
A2	43.93	30	25	22
A3	45.83	26	23	21
A4	38.46	16	11	9
A5	50	6	6	6
A6	42.44	78	60	55
A7	42.76	87	62	68
A8	44.93	57	48	45
A9	44.24	46	34	39
A10	47.15	51	49	42

>TRINITY_DN44387_c0_g1_i2 from sample A8 / Example spliced isoform

GCAGGGACAGATGAATATCTCAACGTGAAGGGGAAGAGACGGCGATATCCCGCAATTATAGTGTTGCTTTCACGGCGGAATTCCCTCTCGTGTAATTATCATATAAACTGTCTGCTAAAATGGCGAAGCCCGTCGGGCTGTTTTCATGGATTTCTACCGCCCAATTCCTACCACATTGCTGGGCAAATCAGTTTACAGAT
ACCCAATAGATATTCAATGCAGGAATATCCGGCTGCTTAACCTGCCTCATCATTCCAGCAAGTTTTACAGGAAATTGCAGGCTTGGAGCAAAAGTTTTGAGAATAATTTTATTGCATATACGAGAATGTCTTCGCCAATTTCAGCAGCTTCCACGTCTGGCATGGAAACTGTAGGAGGAGTAGAGGTTCCAGATTCACAG
TCTGTTGGCGAAAAAGATTTGCTGATTGTAGGACCAGGTGTACTAGGCCGACTGGTAGCAGAAAGCTGGCTAAAGGAAAATCCTTCTTGCAGAGTTGTTGGACAAACAAGGTCGAAGGATCATCATGAAGAGTTACAGCAACTTGGAATCAAACCATGTTTGAAGGGATCGCAAGCACATGAGCTGTTTCCTTTTGTTAT
TTACTGCGCTCCCCCCTCTGGAAGTGGTGAATACCCAGATGATGTCAGGGCAGCCACATTTCAATGGAATGGAGAAGGTGCATTTTTGTTCACATCAAGTAGTGCCGTTTATGCTTGCAATGATAATGGACTGTGCCTTGAGGAATCCCCTACAGTGCCAATTGGGAGTAGTTTTCGGACAGATGTTCTTTTGAAAGCAG
AAAATGAAGTGCTGAAAATTGGTGGTAGTGTGATACGATTATCAGGGCTTTACAATTGGGATAGAGGTGCTCATCTATATTGCCTAGAGAAGGGGACTGTTGAAGCACGCCCAGATCATATTCTCAATCTTATCCATTATGAGGATGCAGCTTCTTTATGCATATCTATATTGAAAGGAAAGTTCAGACGGCGTGTCTTT
ATGGGTTGTGACAATCATCCTTTATCCAGGAAAGATTTAATGGATTATGTGAACAAAAGTGGCAAGTTTGATAAAAAGTTTCAGGGTTTCACAGGTGTTGATGGGCCCTTGGGAAAACGGATGGATAACACAAAAACTTGTAAGGAAATTGGTTGGCAACCAAAGTATCCAAGCTTTGCCCAATTTCTTGGAATTACAGA
GTGAATGCTAAACACATTGTTATGAGCATTTGCAATTTGTTGGTGCGTTTCAAGAGGGCAGTATATCTGCAACTTTCACTTTACCTAATAATATCAATGTTATTCCCCCACGGGGTCCCATTCTTAATCAATGTAACATAAATACCTCACCAAAGACCCATTTCGGTCTTTTGGTGTGTACTTACAAGTACAAAGATTAA
GGCTATATTTTTGTGCCTATAACATCCCTTGCAATGCATGGGACTGCTTTTGTTGGCACCTAAACAATTTCTTTACATTACTTATTGAATGCAGATAGATATCTCATGTCTCAATCTCGTCTGATAACACTGTTTTTAGGAGTGAGCTGCCAAGTAATCTCGAGGTTCCCCAAGCCATTTTTTACATGAATGGTTGTTTC
TTTGAGGTTTATGTATGTGGCTGCAGTCCTATTAGCCAATTTCCTCTCACCAGCACAGGAAATCGCAAGCTTTAAATATGTATAAAACCACTAAAAACCAATGTTTTGCATTCCTTTCAACATATGATACATATCCAACAAGGTATACC

>TRINITY_DN44387_c0_g1_i3 from sample A8 / Example unspliced isoform:

GCAGGGACAGATGAATATCTCAACGTGAAGGGGAAGAGACGGCGATATCCCGCAATTATAGTGTTGCTTTCACGGCGGAATTCCCTCTCGTGTAATTATCATATAAACTGTCTGCTAAAATGGCGAAGCCCGTCGGGCTGTTTTCATGGATTTCTACCGCCCAATTCCTACCACATTGCTGGGCAAATCAGTTTACAGAT
ACCCAATAGATATTCAATGCAGGAATATCCGGCTGCTTAACCTGCCTCATCATTCCAGCAAGTTTTACAGGAAATTGCAGGCTTGGAGCAAAAGTTTTGAGAATAATTTTATTGCATATACGAGAATGTCTTCGCCAATTTCAGCAGCTTCCACGTCTGGAAAATCCTTCTTGCAGAGTTGTTGGACAAACAAGGTCGAA
GGATCATCATGAAGAGTTACAGCAACTTGGAATCAAACCATGTTTGAAGGGATCGCAAGCACATGAGCTGTTTCCTTTTGTTATTTACTGCGCTCCCCCCTCTGGAAGTGGTGAATACCCAGATGATGTCAGgtgaatacccagatattgccagGGCAGCCACATTTCAATGGAATGGAGAAGGTGCATTTTTGTTCACA
TCAAGTAGTGCCGTTTATGCTTGCAATGATAATGGACTGTGCCTTGAGGAATCCCCTACAGTGCCAATTGGGAGTAGTTTTCGGACAGATGTTCTTTTGAAAGCAGAAAATGAAGTGCTGAAAATTGGTGGTAGTGTGATACGATTATCAGGGCTTTACAATTGGGATAGAGGTGCTCATCTATATTGCCTAGAGAAGGG
GACTGTTGAAGCACGCCCAGATCATATTCTCAATCTTATCCATTATGAGGATGCAGCTTCTTTATGCATATCTATATTGAAAGGAAAGTTCAGACGGCGTGTCTTTATGGGTTGTGACAATCATCCTTTATCCAGGAAAGATTTAATGGATTATGTGAACAAAAGTGGCAAGTTTGATAAAAAGTTTCAGGGTTTCACAG
GTGTTGATGGGCCCTTGGGAAAACGGATGGATAACACAAAAACTTGTAAGGAAATTGGTTGGCAACCAAAGTATCCAAGCTTTGCCCAATTTCTTGGAATTACAGAGTGAATGCTAAACACATTGTTATGAGCATTTGCAATTTGTTGGTGCGTTTCAAGAGGGCAGTATATCTGCAACTTTCACTTTACCTAATAATAT
CAATGTTATTCCCCCACGGGGTCCCATTCTTAATCAATGTAACATAAATACCTCACCAAAGACCCATTTCGGTCTTTTGGTGTGTACTTACAAGTACAAAGATTAAGGCTATATTTTTGTGCCTATAACATCCCTTGCAATGCATGGGACTGCTTTTGTTGGCACCTAAACAATTTCTTTACATTACTTATTGAATGCAG
ATAGATATCTCATGTCTCAATCTCGTCTGATAACACTGTTTTTAGGAGTGAGCTGCCAAGTAATCTCGAGGTTCCCCAAGCCATTTTTTACATGAATGGTTGTTTCTTTGAGGTTTATGTATGTGGCTGCAGTCCTATTAGCCAATTTCCTCTCACCAGCACAGGAAATCGCAAGCTTTAAATATGTATAAAACCACTAA
AAACCAATGTTTTGCATTCCTTTCAACATATGATACATATCCAACAAGGTATACC