NA ----> NA

Intron 1 GTATGAAGGT ... ATTTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.69	37	44	49
A2	56.72	29	21	55
A3	55.71	31	20	58
A4	60.32	25	26	50
A5	36.23	22	8	17
A6	32.69	35	5	29
A7	49.07	55	25	81
A8	57.58	14	8	30
A9	58.14	45	33	92
A10	46.6	55	24	72

>TRINITY_DN45547_c0_g2_i3 from sample A10 / Example spliced isoform

AAAGGCTGGCCAGCAAAACAACAGGCTACAATTATTTGCGATGAATACAACACTTGCATTATGAAACTGACTATAACACTCGCAATATGAAACTGAACATACCAATTACATCGATTTGTCTATCACGAGAGCAGCTGTTGTCTGAGAATGCAACATTTTTTAATAGACCAAGCCGAACTCAAAACATGACCTATGGAACC
TAATTCTGCACCGTAAGGCAGCTTCAGTTTCTCAATTATGGTCAAGTTGGGCCTGAGACAGAGAATGGGAGCTCGAGTCATGTTAGAGCCCATGATATTCTACCAATAAACATAATATACATCCACACTCTGCTATCCAAATTCTGATACATCTATTTATTTGTTCTTTGAATTTGCACACGCTCAACTCTTTGACGAAG
GTTTATGCGCGCGAGGATAAGGTTTTTAAGGAGGGTTTCATATTAAGGGTTTTTGAAGCTTGGTGGCTGGCGTTGGTTTTGCTCCTCTGCATTCGAGGTAGAAATCCGGCAAGGGACGAGGTGCAAAGGATTTAAGAAGATGGCAGGGATACCAGAGGATGCAAGTTCAATTCTTGACACGATAAAAGCAGATCGTTCTG
TTTGGCTTATGAAGTGTCCTCAGGTTATTGGTCAAGCATGGAAAGAAATTAATGATTCAGGGCAGCCTTTAGCTAAAATAACTGTGTCAGTTGATCCATGTAAGACAGATGATCAACGCATTGAAGCAAAGGTTGAAGATCCGACAGCACCCTCGGAAATGAGGATAAAGGCGGAAAGGCCAGAGATGTCTTCGACCGTG
AGTCAAACTCAATCAACTCGCAGTATTAAATCAGAGGCAAAGTAGCTGTTGATGGGAAGGTCGAGCATAAATTTGATATGAAGCCTCACAGTCAAAATATTGATGACTACCGGAGGCTATGTCGAGAAAGGACAAACAAATCTAATTTCAGAGCGAGACAAGTGCAGGTTATAGATAATGATCGTGTAGGCTATAATCCC
ATGCAAGGAATGATGGATTTTTCATCAATATTATCAAAGGATAGAAGGGGTTCTGCCGCACCAATTAAGACTCAAGAAATGAAGAGAACCAGAATGGACCGTGGTGAATTAGAGAACATTCTTTTCAAGCTCTTTGAGCGGCAGACCAACTGGACATTAAAACAACTGTTGATGGAAACGGATCAGCCTCAGCAATTTAT
AAAGGAAATATTGAATGATCTTGGCATTTACAACAAAAGGGGTACAAATCAAGGAACATATGAGCTTAAACCTGAATATAAGAAAAGTACTGAAGAAATGGAGGACGAATAGATTGTCTGAGATGTTTTGGCTAGTAGTTTCTTTGTGGTGTACTGATACAAACTTCTTGTGTTTTGTCAGCTCTAAGATTAAGCACCTA
AGCAGAATACACAACTGTATGCTGTAAATTGTACTTGTTATCAAAGACTCCTACATGATTAAAGCAATTTTGTCAAGTTTTCAAACCCTGCATAAATATTTTGAAAATTCGAAACTCATGATGCCGTCGAAGTCTTTTTTCTTTCCATCTTGTAATTTGAGGTTTGTTAGCAAGGCAAATGATAAGTTGTTTCCTCATAC
GGTGGAAAGCGTTGGTCAATATATCCTGGAAGTATTAAGCTACAGGCATATGCCATTTTTTTCTAGTGGTGGATTACATTCTGATAGTAGCTATTGCCATTTTTTTCTATTGGTGGATTACATTCTGATAGTAGCTATTACCATTTTGTTTCTATTGGTGGATTACATTTTGATAGTAGCTATTGCTTTCTGTCTGCTAC
TGTTTCACTTCATGGTTATTCAAAACCAAATTAAAGAAAAAGCTTTCTTAAGGTGGTG

>TRINITY_DN45547_c0_g2_i7 from sample A10 / Example unspliced isoform:

AAAGGCTGGCCAGCAAAACAACAGGCTACAATTATTTGCGATGAATACAACACTTGCATTATGAAACTGACTATAACACTCGCAATATGAAACTGAACATACCAATTACATCGATTTGTCTATCACGAGAGCAGCTGTTGTCTGAGAATGCAACATTTTTTTATAGACCAAGCTGAACTCAAAACATGACCTATGGAACC
TAATTCTGCACCGTAAGGCAGCTTCAGTTTCTCAATTATGGTCAAGTTGGGCCTGAGACAGAGAATGGGAGCTCGAGTCATGTTAGAGCCCATGATATTCTACCAATAAACATAATATACATCCACACTCTGCTATCCAAATTCTGATACATCTATTTATTTGTTCTTTGAATTTGCACACGCTCAACTCTTTGACGAAG
GTTTATGCGCGCGAGGATAAGGTTTTTAAGGAGGGTTTCATgtatgaaggttttaggtgtcaagggtttttcagtatttttttccaagggtttcatgcatgcattgagtgtgtcaagggtttccgaaatttgtacagATTAAGGGTTTTTGAAGCTTGGTGGCTGGCGTTGGTTTTGCTCCTCTGCATTCGAGGTAGAAA
TCCGGCAAGGGACGAGGTGCAAAGGATTTAAGAAGATGGCAGGGATACCAGAGGATGCAAGTTCAATTCTTGACACGATAAAAGCAGATCGTTCTGTTTGGCTTATGAAGTGTCCTCAGGTTATTGGTCAAGCATGGAAAGAAATTAATGATTCAGGGCAGCCTTTAGCTAAAATAACTGTGTCAGTTGATCCATGTAAG
ACAGATGATCAACGCATTGAAGCAAAGGTTGAAGATCCGACAGCACCCTCGGAAATGAGGATAAAGGCGGAAAGGCCAGAGATGTCTTCGACCGTGAGTCAAACTCAATCAACTCGCAGTATTAAATCAGAGTTTATGATGGAGGTCGGTGCAAATGGATCTGTAACTGTACCTAAGAGCTACACCTTGAACATGTCTAA
TGATGTTATCCCCATGTATATCTTTTCGGAGACGAGTGAAGGCAAAGTAGCTGTTGATGGGAAGGTCGAGCATAAATTTGATATGAAGCCTCACAGTCAAAATATTGATGACTACCGGAGGCTATGTCGAGAAAGGACAAACAAATCTAATTTCAGAGCGAGACAAGTGCAGGTTATAGATAATGATCGTGTAGGCTATA
ATCCCATGCAAGGAATGATGGATTTTTCATCAATATTATCAAAGGATAGAAGGGGTTCTGCCGCACCAATTAAGACTCAAGAAATGAAGAGAACCAGAATGGACCGTGGTGAATTAGAGAACATTCTTTTCAAGCTCTTTGAGCGGCAGACCAACTGGACATTAAAACAACTGTTGATGGAAACGGATCAGCCTCAGGGC
CTTGAGTTTGTGTTGCTGACATTGCCCCCTTGTGCCTGCATATTTCTCTCAGAAGCTATTTTACTGGGAACACACCCCCTGTGGCTGGAAGCACATCTCCTGTAGAAATTTCATGAAATGCCACTTCCAAATTTTCACCTCAATCTTTGTAATGGCTAGTCTCTGTTTATTACAATTAACAGAAGATTTCTGCAAGTATG
TAAGAGTATATATTCAATATTCATTGATTGATAAGAATCTAAGTTGAACGAAACTTGGAAACTTTCTAAGTTTCTGAAACGGGAGCATCTGGAAACATGATTCCCCAAAATGGGTATGGCCGAGGATAT

Intron 2 GTAATGAGCT ... CGGATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	54.55	25	30	4
A2	52.22	43	47	2
A3	57.63	25	34	2
A4	59.32	24	35	6
A5	47.06	18	16	10
A6	54.74	62	75	2
A7	51.04	188	196	3
A8	53.27	229	261	2
A9	52.65	170	189	1
A10	50.79	155	160	2

>TRINITY_DN103135_c0_g1_i4 from sample A5 / Example spliced isoform

TCTAGAGCGACTCCATAAACACAATCTTTTAAGATCGAAGGGTCATACGAAGCAGGGAAGGGGTTCGCCGCATGCCAGAAAGGTCTGCAACAAACATTTGATTTCAGGATTTGGTTGCTGCTTTCAATCTTGATACTAAGAGGAATTGAGAAGATGGCGGGGACCCCAGAGGATGCAGGTTCACCATTTGACACTCTAAG
GGCAGATCGTTCTGTTTGGCTTATGAAGTGTCCTCTGGTTATTGGTCAAGCATGGAAAGATGTTAGTGATTCAGGGCAACCTATAGCCAAAATAACCGTGTCAGTTGATCCATGCAAGGCAGATGATCAACCTGTTGAAGCAAAGGTTGAAGATCCAACAGCACCTTCTGCAACGAGGAGAAAGGTGGAAAGGAAACAAA
TGTCTTCTACCAGCAGTCAAACTCAATCGACTCGCAGAATTAAATCAGAGTTTATGATGGAAGTGGCTGCGAATGGATCTGTAACTGCACCTAAGAGCTATTCCTTGAACATGTCTAACGAGATTATACCCATGTATATATTTTCAGAGACAAGTGAAGGCAAGGTAGCTGTTGATGGGAAGGTTGAGCATAAATTTGAT
ATGAAGCCTCACACTCAAAACATTGATGATTACCGAAAGCTATGTCGAGAAAGGACAAACAAATCTAACTTTAAAGCAAGACAAGTGCAGGTTATAGATAATGACCGTGGAGGCTATAATCCCATGCAAGGCATGATGGATTTTTCGACAACATTATCAAAGGATAGAAAGGGTTCTGCTGCACCAGTTAAGGCCCAAGA
AATGAAGAGAACCAGAATGGACCGTGGTGAATTAGAGAACATTCTTTTCAAGCTCTTTGAGCGGCAGACCAACTGGACATTAAAACAACTGTTGATGGAAACGGATCAGCCTCAGGGCCTTGAGTTTGTGTTGCTGACATTGCCCCCTTGTGCCTGCATATTTCTCTCAGAAGCTATTTTACTGGGAACACACCCCCTGT
GGCTGGAAGCACATCTCCTGTAGAAATTTCATGAAATGCCACTTCCAAATTTTCACCTCAATCTTTGTAACAGCTAGTCTCTGTTTATTACAATTAACAGAAGATTTCTGCAAATATGTAAGAG

>TRINITY_DN103135_c0_g1_i5 from sample A5 / Example unspliced isoform:

TCTAGAGCGACTCCATAAACACAATCTTTTAAGATCGAAGGGTCATACGAAGCAGGGAAGGGGTTCGCCGCATGCCAGAAAGGTCTGCAACAAACATTTGATTTCAGGATTTGGTTGCTGCTTTCAATCTTGATACTAAGAGGAATTGAGAAGATGGCGGGGACCCCAGAGGATGCAGGTTCACCATTTGACACTCTAAG
GGCAGATCGTTCTGTTTGGCTTATGAAGTGTCCTCTGGTTATTGGTCAAGCATGGAAAGATGTTAGTGATTCAGGGCAACCTATAGCCAAAATAACCGTGTCAGTTGATCCATGCAAGGCAGATGATCAACCTGTTGAAGCAAAGGTTGAAGATCCAACAGCACCTTCTGCAACGAGGAGAAAGGTGGAAAGGAAACAAA
TGTCTTCTACCAGCAGTCAAACTCAATCGACTCGCAGAATTAAATCAGAGTTTATGATGGAAGTGGCTGCGAATGGATCTGTAACTGCACCTAAGAGCTATTCCTTGAACATGTCTAACGAGATTATACCCATGTATATATTTTCAGAGACAAGTGAAGGCAAGGTAGCTGTTGATGGGAAGGTTGAGCATAAATTTGAT
ATGAAGCCTCACACTCAAAACATTGATGATTACCGAAAGCTATGTCGAGAAAGGACAAACAAATCTAACTTTAAAGCAAGACAAGTGCAGGTTATAGATAATGACCGTGGAGGCTATAATCCCATGCAAGGCATGATGGATTTTTCGACAACATTATCAAAGGATAGAAAGGGTTCTGCTGCACCAGTTAAGGCCCAAGA
AATGAAGAGAACCAGAATGGACCGTGGTGAATTAGAGAACATTCTTTTCAAGCTCTTTGAGCGGCAGACCAACTGGACATTAAAACAACTGTTGATGGAAACGGATCAGCCTCAGgtaatgagctactcattcatgtatttcaaacaagtttgtggctgctgcagtttctggctttttaatttttaataaatttgcttca
atttccaaatgtgtatgacataatcagaattgaattactataagaggtctcatatttaatttcccttgaggcaaaattatgtggaaacggattgcagGGCCTTGAGTTTGTGTTGCTGACATTGCCCCCTTGTGCCTGCATATTTCTCTCAGAAGCTATTTTACTGGGAACACACCCCCTGTGGCTGGAAGCACATCTCC
TGTAGAAATTTCATGAAATGCCACTTCCAAATTTTCACCTCAATCTTTGTAACAGCTAGTCTCTGTTTATTACAATTAACAGAAGATTTCTGCAAATATGTAAGAG