sp|Q56XY2|QQT1_ARATH ----> GPN-loop GTPase QQT1

Intron 1 ATATCCTTCT ... CTGAGATTAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.9	300	61	0
A2	14.61	228	39	0
A3	19.94	253	63	0
A4	18.72	178	41	0
A5	15.63	135	25	0
A6	14.49	1410	239	2
A7	14.58	1588	271	0
A8	16.3	1751.2	341	3
A9	15.61	1648.7	305	1
A10	16.74	1268.5	255	0

>TRINITY_DN104748_c0_g1_i4 from sample A4 / Example spliced isoform

AAAACATCCCGGCAATCTCTCTCTCTCTATATTCTAAAGCCAAATACTTAACGAGACCGTGGTTGACGGACGCAAAGAATACTGGAGCTTGTATTTCGTTCGGTGGCACCATTTTCTTACATTGTCTAAGTTTGCAGTCGCAGGAACAACAGTGGAAAAAATATACCAAAGAAAATGGTATTTGGACAAGTGGTGATAGG
ACCCCCAGGATCGGGGAAAACTACTTACTGCAATGGCATGTCTCAGTTTCTTACCCTTATTGGCAGGAAAGTAGCAGTTATCAACTTGGATCCTGCAAATGATGCTTTGCCCTATGAATGTGCGGTCAACATTGAGGATCTAATCAAGCTTCAAGATGTAATGGCTGAGCATGGACTTGGCCCCAATGGAGGCCTGGTGT
ATTGCATGGATTACTTGGAGAAGAACATAGATTGGTTGCAGTCTAAGCTGGAACCTCTTGCAAAAGATCATTACTTTCTCTTTGACTTTCCGGGGCAGGTTGAGCTCTTCACTCTTCATTCAAATGCCAAGAATATCATTCTTGAAATGACCAGAAAGATGGACTACAGTGTATGTGTGTAGTTAAGACCCTAAGAATGG
GCAATTGCTTCTATAGACCGAGGATTTTAAGCTGAACATCTTCATGATCAACCTAGAAATGTTTAGTTGCAGAAATACTCAAACCACATGAAAAATAAATAGTAATGTGGTGGCTTTTAATTTATATGTTTCCATGTATCATGTTTTATACAAATTCTTTCTATGCAGCTTGCAGCAGTACACTTAGTTGATGCCCATCT
TTGTAGTGATCCTGGGAAATATATCAGTGCTCTGCTGCTGTCTTTGACAACAATGTTGCATCTTGAACTGCCACATATTAATGTATTATCCAAGATGGATCTTATTGAAAGTTATGGAAGGCTCGATTTCAATTTAGAATTCTACACGGACGTGCAGGACTTGTCATATTTACAACAATATATAGATCAGGATCCTCGTT
CTGCTAAATACAGAAAACTTACCAAGGCATTGTGTGAGGTGATTGAAGACTTTTCCTTGGTTAGTTTTGCAACCTTGGACATCCAGGACAAGGAGAGTGTTGGGAATCTTGTTAAGCTTATCGATAAAACCAATGGATATGTCTTTGCTGGTATTGAAGGAGATATTGTTGAGTTTAGCAAAATTGCGGCTGGGCCCTTG
GACTGGGATTATTATAGAACTGCAGCTGTACAGGAGAAGTATATGAAGGATGAGGATGACTGATCACAAATCAGGTCTGCTTGAAGATACCCCTTTGAGATGTCTTACCATTAACTATTCTAAATCGGCTCAAAATGCATTCGTGACATGAACTGTTGGTGTTCTTGTGGAAATCCAACTCGTGGATGCTCAGGTCATAA
GGCATCTTACACATCTCTTCTAAATTTCCATTTTGAATTGCTATCAAGGTGGTATGTAACATGTTTGAGTTATGAAACAGAGCTTATTTTGTTAAATTTGTTACCTTATTCTCCATGACAATTAGGCTCTTGTTGAATTGATGTCAACTGAGCAAAGGCACTGATCTTCAAGGTATTGAGAACTTTCTTTTATAAACTTC
AGATTTTAAACCTGCAAGTTGTCTAGCTTGCTGCTAGTGGTTTTAGAAATTTATTTCATGGTTAATGCAAAGTTCACAATGATGAAG

>TRINITY_DN104748_c0_g1_i2 from sample A4 / Example unspliced isoform:

AAAACATCCCGGCAATCTCTCTCTCTCTATATTCTAAAGCCAAATACTTAACGAGACCGTGGTTGACGGACGCAAAGAATACTGGAGCTTGTATTTCGTTCGGTGGCACCATTTTCTTACATTGTCTAAGTTTGCAGTCGCAGGAACAACAGTGGAAAAAATATACCAAAGAAAATGGTATTTGGACAAGTGGTGATAGG
ACCCCCAGGATCGGGGAAAACTACTTACTGCAATGGCATGTCTCAGTTTCTTACCCTTATTGGCAGGAAAGTAGCAGTTATCAACTTGGATCCTGCAAATGATGCTTTGCCCTATGAATGTGCGGTCAACATTGAGGATCTAATCAAGCTTCAAGATGTAATGGCTGAGCATGGACTTGGCCCCAATGGAGGCCTGGTGT
ATTGCATGGATTACTTGGAGAAGAACATAGATTGGTTGCAGTCTAAGCTGGAACCTCTTGCAAAAGATCATTACTTTCTCTTTGACTTTCCGGGGCAGGTTGAGCTCTTCACTCTTCATTCAAATGCCAAGAATATCATTCTTGAAATGACCAGAAAGATGGACTACAGTCTTGCAGCAGTACACTTAGTTGATGCCCAT
CTTTGTAGTGATCCTGGGAAATATATCAGTGCTCTGCTGCTGTCTTTGACAACAATGTTGCATCTTGAACTGCCACATATTAATGTATTATCCAAGATGGATCTTATTGAAAGTTATGGAAGGCTCGATTTCAATTTAGAATTCTACACGGACGTGCAGGACTTGTCATATTTACAACAATATATAGATCAGGATCCTCG
TTCTGCTAAATACAGAAAACTTACCAAGGCATTGTGTGAGGTGATTGAAGACTTTTCCTTGGTTAGTTTTGCAACCTTGGACATCCAGGACAAGGAGAGTGTTGGGAATCTTGTTAAGCTTATCGATAAAACCAATGGATATGTCTTTGCTGGTATTGAAGGAGATATTGTTGAGTTTAGCAAAATTGCGGCTGGGCCCT
TGGACTGGGATTATTATAGatatccttctgacttaatgcttatgatatgcacattctgtttatactttttgttattttcaaaatcatctttttatcatgttccgtaagttgtcttcctttacagctgagattacAACTGCAGCTGTACAGGAGAAGT

Intron 2 GTATGTGTGT ... TTCTATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	13.18	191	22.7	29
A2	13.94	179	16.3	29
A3	10.25	254	26	29
A4	14.75	156	13.7	27
A5	9.68	56	4.7	6
A6	11.18	461	42.7	58
A7	14.46	621	62.3	105
A8	13.04	467	43	70
A9	12.71	584	51.3	85
A10	11.95	538	48	73

>TRINITY_DN104748_c0_g1_i1 from sample A4 / Example spliced isoform

GTTGACGGACGTAAAGAATACTGTGGTTGACGGACGTAAAGAATACTGGAGCTTGTATTTCGTTCGGTGGCACCATTTTCTTACATTGTCTAAGTTTGCAGTCGCAGGAACAACAGTGGAAAAAATATACCAAAGAAAATGGTATTTGGACAAGTGGTGATAGGACCCCCAGGATCGGGGAAAACTACTTACTGCAATGG
CATGTCTCAGTTTCTTACCCTTATTGGCAGGAAAGTAGCAGTTATCAACTTGGATCCTGCAAATGATGCTTTGCCCTATGAATGTGCGGTCAACATTGAGGATCTAATCAAGCTTCAAGATGTAATGGCTGAGCATGGACTTGGCCCCAATGGAGGCCTGGTGTATTGCATGGATTACTTGGAGAAGAACATAGATTGGT
TGCAGTCTAAGCTGGAACCTCTTGCAAAAGATCATTACTTTCTCTTTGACTTTCCGGGGCAGGTTGAGCTCTTCACTCTTCATTCAAATGCCAAGAATATCATTCTTGAAATGACCAGAAAGATGGACTACAGTCTTGCAGCAGTACACTTAGTTGATGCCCATCTTTGTAGTGATCCTGGGAAATATATCAGTGCTCTG
CTGCTGTCTTTGACAACAATGTTGCATCTTGAACTGCCACATATTAATGTATTATCCAAGATGGATCTTATTGAAAGTTATGGAAGGCTCGATTTCAATTTAGAATTCTACACGGACGTGCAGGACTTGTCATATTTACAACAATATATAGATCAGGATCCTCGTTCTGCTAAATACAGAAAACTTACCAAGGCATTGTG
TGAGGTGATTGAAGACTTTTCCTTGGTTAGTTTTGCAACCTTGGACATCCAGGACAAGGAGAGTGTTGGGAATCTTGTTAAGCTTATCGATAAAACCAATGGATATGTCTTTGCTGGTATTGAAGGAGATATTGTTGAGTTTAGCAAAATTGCGGCTGGGCCCTTGGACTGGGATTATTATAGAACTGCAGCTGTACAGG
AGAAGTATATGAAGGATGAGGATGACTGATCACAAATCAGGTCTGCTTGAAGATACCCCTTTGAGATGTCTTACCATTAACTATTCTAAATCGGCTCAAAATGCATTCGTGACATGAACTGTTGGTGTTCTTGTGGAAATCCAACTCGTGGATGCTCAGGTCATAAGGCATCTTACACATCTCTTCTAAATTTCCATTTT
GAATTGCTATCAAGGTGGTATGTAACATGTTTGAGTTATGAAACAGAGCTTATTTTGTTAAATTTGTTACCTTATTCTCCATGACAATTAGGCTCTTGTTGAATTGATGTCAACTGAGCAAAGGCACTGATCTTCAAGGTATTGAGAACTTTCTTTTATAAACTTCAGATTTTAAACCTGCAAGTTGTCTAGCTTGCTGC
TAGTGGTTTTAGAAATTTATTTCATGGTTAATGCAAAGTTCACAATGATGAAG

>TRINITY_DN104748_c0_g1_i4 from sample A4 / Example unspliced isoform:

AAAACATCCCGGCAATCTCTCTCTCTCTATATTCTAAAGCCAAATACTTAACGAGACCGTGGTTGACGGACGCAAAGAATACTGGAGCTTGTATTTCGTTCGGTGGCACCATTTTCTTACATTGTCTAAGTTTGCAGTCGCAGGAACAACAGTGGAAAAAATATACCAAAGAAAATGGTATTTGGACAAGTGGTGATAGG
ACCCCCAGGATCGGGGAAAACTACTTACTGCAATGGCATGTCTCAGTTTCTTACCCTTATTGGCAGGAAAGTAGCAGTTATCAACTTGGATCCTGCAAATGATGCTTTGCCCTATGAATGTGCGGTCAACATTGAGGATCTAATCAAGCTTCAAGATGTAATGGCTGAGCATGGACTTGGCCCCAATGGAGGCCTGGTGT
ATTGCATGGATTACTTGGAGAAGAACATAGATTGGTTGCAGTCTAAGCTGGAACCTCTTGCAAAAGATCATTACTTTCTCTTTGACTTTCCGGGGCAGGTTGAGCTCTTCACTCTTCATTCAAATGCCAAGAATATCATTCTTGAAATGACCAGAAAGATGGACTACAGTgtatgtgtgtagttaagaccctaagaatgg
gcaattgcttctatagaccgaggattttaagctgaacatcttcatgatcaacctagaaatgtttagttgcagaaatactcaaaccacatgaaaaataaatagtaatgtggtggcttttaatttatatgtttccatgtatcatgttttatacaaattctttctatgcagCTTGCAGCAGTACACTTAGTTGATGCCCATCT
TTGTAGTGATCCTGGGAAATATATCAGTGCTCTGCTGCTGTCTTTGACAACAATGTTGCATCTTGAACTGCCACATATTAATGTATTATCCAAGATGGATCTTATTGAAAGTTATGGAAGGCTCGATTTCAATTTAGAATTCTACACGGACGTGCAGGACTTGTCATATTTACAACAATATATAGATCAGGATCCTCGTT
CTGCTAAATACAGAAAACTTACCAAGGCATTGTGTGAGGTGATTGAAGACTTTTCCTTGGTTAGTTTTGCAACCTTGGACATCCAGGACAAGGAGAGTGTTGGGAATCTTGTTAAGCTTATCGATAAAACCAATGGATATGTCTTTGCTGGTATTGAAGGAGATATTGTTGAGTTTAGCAAAATTGCGGCTGGGCCCTTG
GACTGGGATTATTATAGAACTGCAGCTGTACAGGAGAAGTATATGAAGGATGAGGATGACTGATCACAAATCAGGTCTGCTTGAAGATACCCCTTTGAGATGTCTTACCATTAACTATTCTAAATCGGCTCAAAATGCATTCGTGACATGAACTGTTGGTGTTCTTGTGGAAATCCAACTCGTGGATGCTCAGGTCATAA
GGCATCTTACACATCTCTTCTAAATTTCCATTTTGAATTGCTATCAAGGTGGTATGTAACATGTTTGAGTTATGAAACAGAGCTTATTTTGTTAAATTTGTTACCTTATTCTCCATGACAATTAGGCTCTTGTTGAATTGATGTCAACTGAGCAAAGGCACTGATCTTCAAGGTATTGAGAACTTTCTTTTATAAACTTC
AGATTTTAAACCTGCAAGTTGTCTAGCTTGCTGCTAGTGGTTTTAGAAATTTATTTCATGGTTAATGCAAAGTTCACAATGATGAAG

Intron 3 GTTAGTTTTG ... GGACATCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.46	59	163	181
A2	77.57	36	127	122
A3	77.46	56	195	190
A4	74.09	49	135	145.3
A5	74.04	27	72	82
A6	73.89	232	625	688
A7	72.13	300	736	817.3
A8	72.27	315	792	849.7
A9	72.25	319	785	876
A10	73.57	237	623	696.7

>TRINITY_DN104748_c0_g1_i6 from sample A4 / Example spliced isoform

TGAAGACTTTTCCTTGGACAAGGAGAGTGTTGGGAATCTTGTTAAGCTTATCGATAAAACCAATGGATATGTCTTTGCTGGTATTGAAGGAGATATTGTTGAGTTTAGCAAAATTGCGGCTGGGCCCTTGGACTGGGATTATTATAGAACTGCAGCTGTACAGGAGAAGTATATGAAGGATGAGGATGACTGATCACAAA
TCAGGTCTGCTTGAAGATACCCCTTTGAGATGTCTTACCATTAACTATTCTAAATCGGCTCAAAATGCATTCGTGACATGAACTGTTGGTGTTCTTGTGGAAATCCAACTCGTGGATGCTCAGGTCATAAGGCATCTTACACATCTCTTCTAAATTTCCATTTTGAATTGCTATCAAGGTGGTATGTAACATGTTTGAGT
TATGAAACAGAGCTTATTTTGTTAAATTTGTTACCTTATTCTCCATGACAATTAGGCTCTTGTTGAATTGATGTCAACTGAGCAAAGGCACTGATCTTCAAGGTATTGAGAACTTTCTTTTATAAACTTCAGATTTTAAACCTGCAAGTTGTCTAGCTTGCTGCTAGTGGTTTTAGAAATTTATTTCATGGTTAATGCAA
AGTTCACAATGATGAAG

>TRINITY_DN104748_c0_g1_i1 from sample A4 / Example unspliced isoform:

GTTGACGGACGTAAAGAATACTGTGGTTGACGGACGTAAAGAATACTGGAGCTTGTATTTCGTTCGGTGGCACCATTTTCTTACATTGTCTAAGTTTGCAGTCGCAGGAACAACAGTGGAAAAAATATACCAAAGAAAATGGTATTTGGACAAGTGGTGATAGGACCCCCAGGATCGGGGAAAACTACTTACTGCAATGG
CATGTCTCAGTTTCTTACCCTTATTGGCAGGAAAGTAGCAGTTATCAACTTGGATCCTGCAAATGATGCTTTGCCCTATGAATGTGCGGTCAACATTGAGGATCTAATCAAGCTTCAAGATGTAATGGCTGAGCATGGACTTGGCCCCAATGGAGGCCTGGTGTATTGCATGGATTACTTGGAGAAGAACATAGATTGGT
TGCAGTCTAAGCTGGAACCTCTTGCAAAAGATCATTACTTTCTCTTTGACTTTCCGGGGCAGGTTGAGCTCTTCACTCTTCATTCAAATGCCAAGAATATCATTCTTGAAATGACCAGAAAGATGGACTACAGTCTTGCAGCAGTACACTTAGTTGATGCCCATCTTTGTAGTGATCCTGGGAAATATATCAGTGCTCTG
CTGCTGTCTTTGACAACAATGTTGCATCTTGAACTGCCACATATTAATGTATTATCCAAGATGGATCTTATTGAAAGTTATGGAAGGCTCGATTTCAATTTAGAATTCTACACGGACGTGCAGGACTTGTCATATTTACAACAATATATAGATCAGGATCCTCGTTCTGCTAAATACAGAAAACTTACCAAGGCATTGTG
TGAGGTGATTGAAGACTTTTCCTTGgttagttttgcaaccttggacatccagGACAAGGAGAGTGTTGGGAATCTTGTTAAGCTTATCGATAAAACCAATGGATATGTCTTTGCTGGTATTGAAGGAGATATTGTTGAGTTTAGCAAAATTGCGGCTGGGCCCTTGGACTGGGATTATTATAGAACTGCAGCTGTACAGG
AGAAGTATATGAAGGATGAGGATGACTGATCACAAATCAGGTCTGCTTGAAGATACCCCTTTGAGATGTCTTACCATTAACTATTCTAAATCGGCTCAAAATGCATTCGTGACATGAACTGTTGGTGTTCTTGTGGAAATCCAACTCGTGGATGCTCAGGTCATAAGGCATCTTACACATCTCTTCTAAATTTCCATTTT
GAATTGCTATCAAGGTGGTATGTAACATGTTTGAGTTATGAAACAGAGCTTATTTTGTTAAATTTGTTACCTTATTCTCCATGACAATTAGGCTCTTGTTGAATTGATGTCAACTGAGCAAAGGCACTGATCTTCAAGGTATTGAGAACTTTCTTTTATAAACTTCAGATTTTAAACCTGCAAGTTGTCTAGCTTGCTGC
TAGTGGTTTTAGAAATTTATTTCATGGTTAATGCAAAGTTCACAATGATGAAG

Intron 4 GCCTGGTGTA ... AAGAACATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.17	115	181	231
A2	58.35	149	165.5	252
A3	55.88	150	155	225
A4	66.73	95	182	199
A5	71.3	32	81	78
A6	61.91	275	389	505
A7	62.58	363	553	661
A8	59.97	267	348.5	451.5
A9	61.99	340	497	612
A10	61.35	301	431.5	524

>TRINITY_DN113233_c0_g2_i4 from sample A2 / Example spliced isoform

TTAAAACATCCCGGCAATCTCTCTCTCTCTATATTCTAAAGCCAAATACTTAACGAGACCGTGGTTGACGGACGCAAAGAATACTGGAGCTTGTATTTCGTTCGGTGGCACCATTTTCTTACATTGTCTAAGTTTGCAGTTGCAGGAACAACAGTGGAAAAAATATACCTAAGAAAATGGTATTTGGACAAGTGGTGATA
GGACCCCCAGGATCGGGGAAAACTACTTACTGCAATGGCATGTCTCAGTTTCTTACCCTTATTGGCAGGAAAGTAGCAGTTATCAACTTGGATCCTGCAAATGATGCTTTGCCCTATGAATGTGCGGTCAACATTGAGGATCTAATCAAGCTTCAAGATGTAATGGCTGAGCATGGACTTGGCCCCAATGGAGATTGGTT
GCAGTCTAAGCTGGAACCTCTTGCAAAAGATCATTACTTTCTCTTTGACTTTCCGGGGCAGGTTGAGCTCTTCACTCTTCATTCAAATGCCAAGAATATCATTCTTGAAATGACCAGAAAGATGGACTACAGTCTTGCAGCAGTACACTTAGTTGATGCCCATCTTTGTAGTGATCCTGGGAAATATATCAGTGCTCTGC
TGCTGTCTTTGACAACAATGTTGCATCTTGAACTGCCACATATTAATGTATTATCCAAGATGGATCTTATTGAAAGTTATGGAAGGCTCGATTTCAATTTAGAATTCTACACGGATGTGCAGGACTTGTCATATTTACAACAATATATAGATCAGGATCCTCGTTCTGCTAAATACAGAAAACTTACCAAGGCATTGTGT
GAGGTGATTGAAGACTTTTCCTTGGTTAGTTTTGCAACCTTGGACATCCAGGACAAGGAGAGTGTTGGGAATCTTGTTAAGCTTATCGATAAAACCAATGGATATGTCTTTGCTGGTATTGAAGGAGATATTGTTGAGTTTAGCAAAATTGCAGCTGGGCCCTTGGACTGGGATTATTATAGAACTGCAGCTGTACAGGA
GAAGTATATGAAGGATGAGGATGACTGATCACAAATCAGGTCTGCTTGAAGATACCCCTTTGAGATGTCTTACCATTAACTATTCTAAATCGGCTCAAAATGCATTCGTGACATGAACTGTTGGTGTTCTTGTGGAAATCCAACTCGTGGATGCTCAGGTCATAAGGCATCTTACACATCTCTTCTCAATTTCCATTTTG
AATTGCTATCAAGGTGGTATGTAACATGTTTGAGTTATGAAACAGAGCTTATTTTGTTAAATTTGTTACCTTATTCTCCGTGACAATTAGGCTCTTGTTGAATTGATGCCAACTGAGCAAAGTCACTGATCTTCAAGGTATTGAGAACTTTCTTTTATAAACTTCAGATTTTAAACCTGCAAGTTGTCTAGCTTGCTGCT
AGTGGTTTTAGAAATTTATTTCATGG

>TRINITY_DN113233_c0_g2_i3 from sample A2 / Example unspliced isoform:

CTTTTTTGGCAAAAATTCAAGTTTGAGGCCAATCCAGGGCTTCCTGGAGCTTCCTAGTTTCGTTGGGCAACTTACACTTGCATTTTTTGGAAAAAATATACCTAAGAAAATGGTATTTGGACAAGTGGTGATAGGACCCCCAGGATCGGGGAAAACTACTTACTGCAATGGCATGTCTCAGTTTCTTACCCTTATTGGCA
GGAAAGTAGCAGTTATCAACTTGGATCCTGCAAATGATGCTTTGCCCTATGAATGTGCGGTCAACATTGAGGATCTAATCAAGCTTCAAGATGTAATGGCTGAGCATGGACTTGGCCCCAATGGAGgcctggtgtattgcatggattacttggagaagaacatagATTGGTTGCAGTCTAAGCTGGAACCTCTTGCAAAA
GATCATTACTTTCTCTTTGACTTTCCGGGGCAGGTTGAGCTCTTCACTCTTCATTCAAATGCCAAGAATATCATTCTTGAAATGACCAGAAAGATGGACTACAGTCTTGCAGCAGTACACTTAGTTGATGCCCATCTTTGTAGTGATCCTGGGAAATATATCAGTGCTCTGCTGCTGTCTTTGACAACAATGTTGCATCT
TGAACTGCCACATATTAATGTATTATCCAAGATGGATCTTATTGAAAGTTATGGAAGGCTCGATTTCAATTTAGAATTCTACACGGATGTGCAGGACTTGTCATATTTACAACAATATATAGATCAGGATCCTCGTTCTGCTAAATACAGAAAACTTACCAAGGCATTGTGTGAGGTGATTGAAGACTTTTCCTTGGTTA
GTTTTGCAACCTTGGACATCCAGGACAAGGAGAGTGTTGGGAATCTTGTTAAGCTTATCGATAAAACCAATGGATATGTCTTTGCTGGTATTGAAGGAGATATTGTTGAGTTTAGCAAAATTGCAGCTGGGCCCTTGGACTGGGATTATTATAGAACTGCAGCTGTACAGGAGAAGTATATGAAGGATGAGGATGACTGA
TCACAAATCAGGTCTGCTTGAAGATACCCCTTTGAGATGTCTTACCATTAACTATTCTAAATCGGCTCAAAATGCATTCGTGACATGAACTGTTGGTGTTCTTGTGGAAATCCAACTCGTGGATGCTCAGGTCATAAGGCATCTTACACATCTCTTCTCAATTTCCATTTTGAATTGCTATCAAGGTGGTATGTAACATG
TTTGAGTTATGAAACAGAGCTTATTTTGTTAAATTTGTTACCTTATTCTCCGTGACAATTAGGCTCTTGTTGAATTGATGCCAACTGAGCAAAGTCACTGATCTTCAAGGTATTGAGAACTTTCTTTTATAAACTTCAGATTTTAAACCTGCAAGTTGTCTAGCTTGCTGCTAGTGGTTTTAGAAATTTATTTCATGG

Intron 5 ATATCCTTCT ... CTGAGATTAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.09	291.9	41.1	100.4
A2	21.41	223.8	25.6	65.6
A3	17.17	248.6	44.2	54.3
A4	20.33	172.6	26.9	46.7
A5	19.08	131	15.8	32.6
A6	22.45	1367.4	157.3	423.8
A7	22.62	1542.1	175.8	483.8
A8	20.98	1708.4	222.3	484.1
A9	21.07	1605.8	201.4	457.7
A10	21.33	1236.6	167.8	358.3

>TRINITY_DN117574_c0_g1_i2 from sample A1 / Example spliced isoform

ATTGTCGAGAATATTTGTGCAAAATTTTATATATCTAGACTTTAAAATTGGCATTCACTATCATCCTTTGTTTATATGAACAAGGCCTGGTGTATTGCATGGATTACTTGGAGAAGAACATAGATTGGTTGCAGTCTAAGCTGGAACCTCTTGCAAAAGATCATTACTTTCTCTTTGACTTTCCGGGGCAGGTTGAGCTC
TTCACTCTTCATTCAAATGCCAAGAATATCATTCTTGAAATGACCAGAAAGATGGACTACAGTCTTGCAGCAGTACACTTAGTTGATGCCCATCTTTGTAGTGATCCTGGGAAATATATCAGTGCTCTGCTGCTGTCTTTGACAACAATGTTGCATCTTGAACTGCCACATATTAATGTATTATCCAAGATGGATCTTAT
TGAAAGTTATGGAAGGCTCGATTTCAATTTAGAATTCTACACGGACGTGCAGGACTTGTCATATTTACAACAATATATAGATCAGGATCCTCGTTCTGCTAAATACAGAAAACTTACCAAGGCATTGTGTGAGGTGATTGAAGACTTTTCCTTGGTTAGTTTTGCAACCTTGGACATCCAGGACAAGGAGAGTGTTGGGA
ATCTTGTTAAGCTTATCGATAAAACCAATGGATATGTCTTTGCTGGTATTGAAGGAGATATTGTTGAGTTTAGCAAAATTGCAGCTGGGCCCTTGGACTGGGATTATTATAGAACTGCAGCTGTACAGGAGAAGTATATGAAGGATGAGGATGACTGATCACAAATCAGGTCTGCTTGAAGATACTCCTTTGAGATGTCT
TACCATTAACTATTGTAAATCTGCTCAAAATGCATTCGTGACATGAACTGTTGGTGTTGTGGAAATCCAACTCGTGGATGCTCAGGTCATAAGGCATCTTACACATCTCTTCTCAATTTCCATTTTGAATTGCTATCAAGGTGGTATGTAACATGTTTGAGTTATGAAACAGAGCTTATTTTGTTAAATTTGTTACCTTA
TTCTCCATGACAATTAGGCTCTTGTTGAATTGATGCCAACTGAGCAAAGTCACTGATCTTCAAGGTATTGAGAACTTTCTTTTATAAACTTCAGATTTTAAACCTGCAAGTTGTCTAGCTTGCTGCTAGTGGTTTTAGAAATTTATTTCATGGTTAATGCAAAGTTCACAATGATGAAG

>TRINITY_DN117574_c0_g1_i1 from sample A1 / Example unspliced isoform:

AGGCCTGGTGTATTGCATGGATTACTTGGAGAAGAACATAGATTGGTTGCAGTCTAAGCTGGAACCTCTTGCAAAAGATCATTACTTTCTCTTTGACTTTCCGGGGCAGGTTGAGCTCTTCACTCTTCATTCAAATGCCAAGAATATCATTCTTGAAATGACCAGAAAGATGGACTACAGTCTTGCAGCAGTACACTTAG
TTGATGCCCATCTTTGTAGTGATCCTGGGAAATATATCAGTGCTCTGCTGCTGTCTTTGACAACAATGTTGCATCTTGAACTGCCACATATTAATGTATTATCCAAGATGGATCTTATTGAAAGTTATGGAAGGCTCGATTTCAATTTAGAATTCTACACGGACGTGCAGGACTTGTCATATTTACAACAATATATAGAT
CAGGATCCTCGTTCTGCTAAATACAGAAAACTTACCAAGGCATTGTGTGAGGTGATTGAAGACTTTTCCTTGGTTAGTTTTGCAACCTTGGACATCCAGGACAAGGAGAGTGTTGGGAATCTTGTTAAGCTTATCGATAAAACCAATGGATATGTCTTTGCTGGTATTGAAGGAGATATTGTTGAGTTTAGCAAAATTGC
AGCTGGGCCCTTGGACTGGGATTATTATAGatatccttctgacttaatgcttatgatatgcacattctgtttatactttttgttattttcaaaatcatctttttatccgtaagttgtcttcctttacagctgagattacAACTGCAGCTGTACAGGAGAAGT