sp|Q9FKT0|ARP8_ARATH ----> Actin-related protein 8

Intron 1 GTAAGATTGG ... TGCTGCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.54	10	0	16
A2	72.73	6	0	16
A3	67.1	8	1	16.3
A4	72.9	7	0.3	19
A5	100	0	0	6.2
A6	72.73	3	0	8
A7	80.25	10	2	40.7
A8	81.43	3	0	13.2
A9	89.66	3	0	26
A10	80.21	6	0	24.3

>TRINITY_DN107496_c0_g1_i8 from sample A3 / Example spliced isoform

AAACCAGCGACGTGGCAACAGCATACGCATCCATACTCCAGGCCATGCTCTCCATTGGTTTGGAAATCAGTGACAATATGCCCATCTTCTTCTCAAAGTAACGCTGATTTGGACAGTGGCAGCATTGCACCTGCAGAGAAATTGCCCCTAGATGTGTTCTTGCTGATATTGAAGCGCCTCGGCCCAAGGAATTCCGCGTG
GGCTGCTTTGGTTTGCAGGGCATGGAGGTCGATGGTGGCGGACGAGAGGGTCTGGCTCTTCTTTCTTCGGGAGGAGGCCGGGGAGATGTGGGAGGCTGTGCTTTTCGCCGAGACTTACTTACGGTCCGGGTTTCCTCTCAGGTCTGTTGTTACGGATGTGTATGGCACCCTACCTTTTCAGCTAATATATAGTCAGCGGA
AACAATTGCAGGGGTCTGTCATCATAGATGGTGGATCAGGGTATTGCAAGTTTGGGCAGAGCAAATATTCAGGACCATCTGGAAGGTCATCCACTTTTCTAGAGTATGGGAATATTGAATCTCCAATGTATTCGAGGCTTAGACATTTTTTCTTAGCAATTTATAACAGGTTGCAGGTAAAGCCATCAACACAACCTATC
ATTGTTTCCACTCCTATCAGCCATTGTGACGACACAGAGTCTGCTAATGCAGCTAGGCGACAATTGCGAGAAGCAATATATACAGTGTTGTTTGATATGAATGTACCTGCTGTATGTGCAATCGATCAGGCTGTTTTAGCTCTATATGCTGCACGGCAAACATCAGGAATAGTTGTTAACATTGGTTTTCAAGTCACTTC
TGTTGTTCCAATCTTATGTGGTAAAGTCAAGCGTAATGTGGGCGTGGAAATTATGGGCCAGGGAGCATTGCGACTCACGAGTTATCTTAGTGAATTAATGCATGAAAGAGGAATTGGTTTTTCTTCTATGTACACTGTATCCACTCTTAAGGAGAATTTGTGTTATGTAGCCGAAGATTATCATGCAGAATTGTCCAAGG
ATGTAGAAGCATCCTATACGGTTGGGGATGAAGGAGTTTTTACTCTATCACAAGAGCGTTTCCAAACAGGAGAGATCCTTTTCCAACCCCAGCTTGGTGGAATACGAACTATGGGCTTGCATCGAGCAGTGGCCCTCTGTATTGAGCATTGTAATACTTCTGAAGTAGGAAAGGTTGCAGATGAAGGATGGTTCAAGACT
ATTGTTTTGGCAGGTGGAAGTGCATGTTTGCCTGGCCTAGCTGTCTTATGTGGTAAAGTCAAGCGTAATGTGGGCGTGGAAATTATGGGCCAGGGAGCATTGCGACTCACGAGTTATCTTAGTGAATTAATGCATGAAAGAGGAATTGGTTTTTCTTCTATGTACACTGTATCCACTCTTAAGGAGAATTTGTGTT

>TRINITY_DN107496_c0_g1_i4 from sample A3 / Example unspliced isoform:

CCAGGCCATGCTCTCCATTgtaagattggttaaaatccgagggatcatcgttcgttttcttgaattgccattgccagacaaaatgtgaatttcaccagaacatgctagagatcattactatctctgttttcggggaaaattttaatggccaagctgttgcgcagGGTTTGGAAATCAGTGACAATATGCCCATCTTCTTC
TCAAAGTAACGCTGATTTGGACAGTGGCAGCATTGCACCTGCAGAGAAATTGCCCCTAGATGTGTTCTTGCTGATATTGAAGCGCCTCGGCCCAAGGAATTCCGCGTGGGCTGCTTTGGTTTGCAGGGCATGGAGGTCGATGGTGGCGGACGAGAGGGTCTGGCTCTTCTTTCTTCGGGAGGAGGCCGGGGAGATGTGGG
AGGCTGTGCTTTTCGCCGAGACTTACTTACGGTCCGGGTTTCCTCTCAGGTCTGTTGTTACGGATGTGTATGGCACCCTACCTTTTCAGCTAATATATAGTCAGCGGAAACAATTGCAGGGGTCTGTCATCATAGATGGTGGATCAGGGTATTGCAAGTTTGGGCAGAGCAAATATTCAGGACCATCTGGAAGGTCATCC
ACTTTTCTAGAGTATGGGAATATTGAATCTCCAATGTATTCGAGGCTTAGACATTTTTTCTTAGCAATTTATAACAGGTTGCAGGTAAAGCCATCAACACAACCTATCATTGTTTCCACTCCTATCAGCCATTGTGACGGTTAGTATTTCTTATTCTTTCATTTATCAGTTTATTGTTGCCCTATCTCCAATCAGGACTT
TTTACTTCCCATTATCTTTTCATTGTTTAATCCC

Intron 2 GTATGTCATT ... GGTGGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.18	25.9	5.8	5
A2	20.49	34.9	12.4	9
A3	18.75	13	5.1	3
A4	28.4	10.1	2.2	4
A5	42.86	4	3.7	3
A6	21.74	54	10.9	15
A7	21.87	60.7	15.5	17
A8	21.28	99.9	22.6	27
A9	19.2	58.9	16.5	14
A10	20.65	53.8	10.9	14

>TRINITY_DN44654_c0_g2_i5 from sample A7 / Example spliced isoform

CCAGGCCATGCTCTCCATTGTAAGATTGGTTAAAATCCGAGGGATCATCGTTCGTTTTCTTGAATTGCCATTGCCAGACAAAATGTGAATTTCACCAGAACATGTTAGAGATCATTACTATCTCTGTTTTCGGCGAAAATTTTAATGGCCAAGCTGCTGCGCAGGGTTTGGAAATCAGTGACAATATGCCCATCTTCTTC
TCAAAGTAACGCTGATTTGGACAGTGGCAGCATTGCACCTGCAGAGAAATTGCCCCTAGATGTGTTCTTGCTGATATTGAAGCGCCTCGGCCCAAGGAATTCCGCGTGGGCTGCTTTGGTTTGCAGGGCATGGAGGTCGATGGTGGCGGACGAGAGGGTCTGGCTCTTCTTTCTTCGGGAGGAGGCCGGGGAGATGTGGG
AGGCTGTGCTTTTCGCCGAGACTTACTTACGGTCCGGGTTTCCTCTCAGGTCTGTTGTTACGGATGTGTATGGCACCCTACCTTTTCAGCTAATATATAGTCAGCGGAAACAATTGCAGGGGTCTGTCATCATAGATGGTGGATCAGGGTATTGCAAGTTTGGGCAGAGCAAATATTCAGGACCATCTGGAAGGAGTATG
GGAATATTGAATCTCCAATGTATTCGAGGCTTAGACATTTTTTCTTAGCAATTTATAACAGGTTGCAGGTAAAGCCATCAACACAACCTATCATTGTTTCCACTCCTATCAGCCATTGTGACGACACAGAGTCTGCTAATGCAGCTAGGCGACAATTGCGAGAAGCAATATATACAGTGTTGTTTGATATGAATGTACCT
GCTGTATGTGCAATCGATCAGGCTGTTTTAGCTCTATATGCTGCACGGCAAACATCAGGAATAGTTGTTAACATTGGTTTTCAAGTCACTTCTGTTGTTCCAATCTTATGTGGTAAAGTCAAGCGTAATGTGGGCGTGGAAATTATGGGCCAGGGAGCATTGCGACTCACGAGTTATCTTAGTGAATTAATGCATGAAAG
AGGAATTGGTTTTTCTTCTATGTACACTGTATCCACTCTTAAGGAGAATTTGTGTTATGTAGCCGAAGATTATCATGCAGAATTGTCCAAGGATGTAGAAGCATCCTATACGGTTGGGGATGAAGGAGTTTTTACTCTATCACAAGAGCGTTTCCAAACAGGAGAGATCCTTTTCCAACCCCAGCTTGGTGGAATACGAA
CTATGGGCTTGCATCGAGCAGTGGCCCTCTGTATTGAGCATTGTAATACTTCTGAAGTAGGAAAGGTTGCAGATGAAGGATGGTTCAAGACTATTGTTTTGGCAGGTGGAAGTGCATGTTTGCCTGGCCTAGCTGGAAGGTTAGAGAAAGAACTGCATCAAGCATTGCCAAGTACAGTAGCAAAAGGACTTAAAGTGCTT
TCCCCTCCTTATGGATCTGAGTCTACTTGGTTTGGAGCAAAGATTATCAGTAATATGAGTACTTATCCTCAGGTATGGTGCATACGAAAAAAACAGTTTCGACGTTATGGTGTGAATGCTATCCATGCCATGACTCTTGATGACAACTGAACTAGTCAGCTGATCAGGGAGAATATACGACAGTTTCATGGCAGTGAATA
TATTTGTGAGTGTGGCATCGAGAAGAAACGTCCAAGCCAATTACCCTATTGGAATGGTTTCTACAATTTTAAAATTTTCTCTCTTATTGCATAGTGTCTAGCACTTGTGCTTGTATTTTAGCAAACTGGCATTGTCTATTTGTAAATATGTATTATAAATTTCAAAAGAAAATACAGAGCTTTCAAAAGATGAAAATACC
AGAGCTGAACAAGGGAGATGCTGTTCTTGTAGCACATTGAATGAATGCACAAGTTAACCATGTCTATGTGATGTAGCTGCAAGAATAAAGGCTTCTATTGATAAATATGTATTATAAATTTCAAAAGATGAAAACGCCAGAGCTTGAAAGAGAGCAAAGGAGATGATCTATTGTAGCACATCGAGTCAATGCATAAATTA
ACAATGTATATGTGATGTAGCTGCAAGAATAAAGACTTCATTAGAAGCATAGAAATATAACGGTCTCCTTCGGCTTGTAATGAAGCGTACCGTCTTCATTAGAGTACGAGTTGTTAAAAGTTGTTTCCTTTTGTTCGAGTCTATTTCATTTGAGAAGGCATACAAAAACAAATATAAGCTTTCATTATCTTTAATTTCAT
TCACTAGATAGTGCTCTATTTTCTTTGGTATTAATCACTTTTGCC

>TRINITY_DN44654_c0_g2_i3 from sample A7 / Example unspliced isoform:

TTGGAGCAAAGATTATCAGTAATgtatgtcattagtactgatgtattatgtgcatttttaaccttgatagtttcaggcaacttgctctatactatttggatgtttttcatatgatcttattcaagtgcaatcggtggcacagATGAGTACTTATCCTCAGGTATGGTGCATACGAAAAAAACAGTTTCGACGTTATGGTG
TGAATGCTATCCATGCCATGACTCTTGATGACAACTGAACTAGTCAGCTGATCAGGGAGAATATACGACAGTTTCATGGCAGTGAATATATTTTGTGAGTGTGGCATCGAGAAGAAACGTCCAAGCCAATTACCCTATTGGAATGGTTTCTACAATTTTAAAATTTTCTCTCTTATTGCATAGTGTCTAGCACTTGTGCT
TGTATTTTAGCAAACTGGCATTGTCTATTTGTAAATATGTATTATAAATTTCAAAAGAAAATACAGAGCTTTCAAAAGATGAAAATACCAGAGCTGAACAAGGGAGATGCTGTTCTTGTAGCACATTGAATGAATGCACAAGTTAACCATGTCTATGTGATGTAGCTGCAAGAATAAAGGCTTCTATTGATAAATATGTA
TTATAAATTTCAAAAGATGAAAACGCCAGAGCTTGAAAGAGAGCAAAGGAGATGATCTATTGTAGCACATCGAGTCAATGCATAAATTAACAATGTATATGTGATGTAGCTGCAAGAATAAAGACTTCATTAGAAGCATAGAAATATAACGGTCTCCTTCGGCTTGTAATGAAGCGTACCGTCTTCATTAGAGTACGAGT
TGTTAAAAGTTGTTTCCTTTTGTTCGAGTCTATTTCATTTGAGAAGGCATACAAAAACAAATATAAGCTTTCATTATCTTTAATTTCATTCACTAGATAGTGCTCTATTTTCTTTGGTATTAATCACTTTTGCC