sp|Q9C5W0|DGAT3_ARATH ----> Diacylglycerol O-acyltransferase 3

Intron 1 GTGCGATATA ... TGTGAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.47	13	24.5	44
A2	70.58	8	14.5	24
A3	66.38	20	39	40
A4	71.14	11	19.5	35
A5	72.28	4	9	12
A6	75.52	17	27	78
A7	61.78	50	43	119
A8	72.4	40	63	147
A9	84.14	13	39	99
A10	62.91	46	39.5	117

>TRINITY_DN116005_c0_g1_i9 from sample A3 / Example spliced isoform

GTATGAGATCCAGGTGGTGGATATGGCTCTGCTCCACCCCACACAACAAGTCGGACGGATTTTAGAGGCCTCTGAGCAAAACCCCTCGGTGCTGCTAGAAAGTCCTCCAAATATAGCTGAGGTTAATGGAGATGAAAAGGCGCATGTGGAATTTGCAGAGGCTAGTGATGGATTGACGTTGGATTCAACGGTGGGCATTC
CAATGCAGGAGGCGAACAGTGTGATAAAAGTATGTATGGGTGGCAAGTGCAAGAAGGCGGGGTCTGAGATGTTGTTGGAAGCGTTTGAAAAGAGAATAAGGAAAGCGGGGATGGAAGCAATGGGGTGCAAGTGCATGGGCAAGTGCCGAAATGCTCTTGCAATAACAGAGCAAGATGGCTTTCATGGCGGCGAGGGAGTA
GTGCATAAGGCTTCGACATTAGGGATGTCGATCTGATGTGCAATAACCCCAACTACCAAAGAAGGCCAAAGGGAGCTGGCCAGCTCCTCCGCACCTAACGGTTGAATATATTGGAGATCATAGAAGCCCGAAGATTCGGGGACTCAGTATCAGTAATTCGCTCACACCGCGCTCAGGCTCTAACTCGTGAGCACGCTGAT
TGCCCCAGTTTTAAGCTGGTCATCGTAGGCAGTGGAGGAAATGGGAAACATACTTTGTGAAGCGTCATCTTACCTGGGAGTTTGAGAAGAAATAAGAATGTGAGTATAAGATTTAAATTTGTTTTTATTATATTAGAACCTCCCTATACGATGGGTATTTTGTTCAACGGGATATTTTGTTACTGCATGCTCAGATGCTA
TT

>TRINITY_DN116005_c0_g1_i6 from sample A3 / Example unspliced isoform:

GTATGAGATCCAGGTGGTGGATATGGCTCTGCTCCACCCCACACAACAAGTCGGACGGATTTTAGAGGCCTCTGAGCAAAACCCCTCGGTGCTGCTAGAAAGTCCTCCAAATATAGCTGAGGTTAATGGAGATGAAAAGGCGCATGTGGAATTTGCAGAGGCTAGTGATGGATTGACGTTGGATTCAACGGTGGGCATTC
CAATGCAGGAGGCGAACAGTGTGATAAAAGTATGTATGGGTGGCAAGTGCAAGAAGGCGGGGTCTGAGATGTTGTTGGAAGCGTTTGAAAAGAGAATAAGGAAAGCGGGGATGGAAGCAATGGGGTGCAAGTGCATGGGCAAGTGCCGAAATGCTCTTGCAATAACAGAGCAAGATGGCTTTCATGGCGGCGAGGGAGTA
GTGCATAAGGCTTCGACATTAGGGATGTCGATCTGATGTGCAATAACCCCAACTACCAAAGAAGGCCAAAGGGAGCTGGCCAGCTCCTCCGCACCTAACGGTTGAATATATTGGAGATCATAGAAGCCCGAAGATTCGGGGACTCAGTATCAGTAATTCGCTCACACCGCGCTCAGGCTCTACCTCGTGAGCACGCTGAT
TGCCCCAGTTTTAAGCTGGTCATCGTAGGCAGTGGAGGAAATGgtgcgatataaatagggtttatgttcagttttgtctgttttaactttgaatcgcgattgactgttcaaatttttgtgaatcagGGAAACATACTTTGTGAAGCGTCATCTTACCTGGGAGTTTGAGAAGAAATAAGAATGTGAGTATAAGATTTAAA
TTTGTTTTTATTATATTAGAACCTCCCTATACGATGGGTATTTTGTTCAACGGGATATTTTGTTACTGCATGCTCAGATGCTATT

Intron 2 GTATCATTTC ... TTATGTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48.84	22	13	29
A2	44.62	18	13	16
A3	48.57	27	16	35
A4	66.67	7	6	22
A5	48.72	10	10	9
A6	38.75	83	28	77
A7	32.99	130	29	99
A8	33.73	165	40	128
A9	37.56	128	42	112
A10	29.15	158	30	100

>TRINITY_DN116005_c0_g1_i6 from sample A3 / Example spliced isoform

GTATGAGATCCAGGTGGTGGATATGGCTCTGCTCCACCCCACACAACAAGTCGGACGGATTTTAGAGGCCTCTGAGCAAAACCCCTCGGTGCTGCTAGAAAGTCCTCCAAATATAGCTGAGGTTAATGGAGATGAAAAGGCGCATGTGGAATTTGCAGAGGCTAGTGATGGATTGACGTTGGATTCAACGGTGGGCATTC
CAATGCAGGAGGCGAACAGTGTGATAAAAGTATGTATGGGTGGCAAGTGCAAGAAGGCGGGGTCTGAGATGTTGTTGGAAGCGTTTGAAAAGAGAATAAGGAAAGCGGGGATGGAAGCAATGGGGTGCAAGTGCATGGGCAAGTGCCGAAATGCTCTTGCAATAACAGAGCAAGATGGCTTTCATGGCGGCGAGGGAGTA
GTGCATAAGGCTTCGACATTAGGGATGTCGATCTGATGTGCAATAACCCCAACTACCAAAGAAGGCCAAAGGGAGCTGGCCAGCTCCTCCGCACCTAACGGTTGAATATATTGGAGATCATAGAAGCCCGAAGATTCGGGGACTCAGTATCAGTAATTCGCTCACACCGCGCTCAGGCTCTACCTCGTGAGCACGCTGAT
TGCCCCAGTTTTAAGCTGGTCATCGTAGGCAGTGGAGGAAATGGTGCGATATAAATAGGGTTTATGTTCAGTTTTGTCTGTTTTAACTTTGAATCGCGATTGACTGTTCAAATTTTTGTGAATCAGGGAAACATACTTTGTGAAGCGTCATCTTACCTGGGAGTTTGAGAAGAAATAAGAATGTGAGTATAAGATTTAAA
TTTGTTTTTATTATATTAGAACCTCCCTATACGATGGGTATTTTGTTCAACGGGATATTTTGTTACTGCATGCTCAGATGCTATT

>TRINITY_DN116005_c0_g1_i17 from sample A3 / Example unspliced isoform:

GTATGAGATCCAGGTGGTGGATATGGCTCTGCTCCACCCCACACAACAAGTCGGACGGATTTTAGAGGCCTCTGAGCAAAACCCCTCGGTGCTGCTAGAAAGTCCTCCAAATATAGCTGAGGTTAATGGAGATGAAAAGGCGCATGTGGAATTTGCAGAGGCTAGTGATGGATTGACGTTGGATTCAACGGTGGGCATTC
CAATGCAGGAGGCGAACAGTGTGATAAAAGTATGTATGGGTGGCAAGTGCAAGAAGGCGGGGTCTGAGATGTTGTTGGAAGCGTTTGAAAAGAGAATAAGGAAAGCGGGGATGGAAGCAATGGGGTGCAAGTGCATGGGCAAGTGCCGAAATGCTCTTGCAATAACAGAGCAAGATGGCTTTCATGGCGGCGAGGGAGTA
GTGCATAAGGCTTCGACATTAGGGATGTCGATCTGATGTGCAATAACCCCAACTACCAAAGAAGGCCAAAGGGAGCTGGCCAGCTCCTCCGCACCTAACGGTTGAATATATTGGAGATCATAGAAGCCCGAAGATTCGGGGACTCAGTATCAGTAATTCGCTCACACCGCGCTCAGgtatcatttccattgtccattgtc
tcaaactactatttccattgtcttgcggtctgaaaccattgttcaggtagttttagaatcacgattaagcattcaaactgtaattgatcagatttagggaaaatatggtcggaataaaggagtgatttaacggcccgttactccaagcccattcaacttgtttttaaaaccgtttttggctggtttgaattcctaaaaca
ttggtgtatttattttctgattatctttttgggattcttttatgtttagGCTCTACCTCGTGAGCACGCTGATTGCCCCAGTTTTAAGCTGGTCATCGTAGGCAGTGGAGGAAATGGTGCGATATAAATAGGGTTTATGTTCAGTTTTGTCTGTTTTAACTTTGAATCGCGATTGACTGTTCAAATTTTTGTGAATCAGG
GAAACATACTTTGTGAAGCGTCATCTTACCTGGGAGTTTGAGAAGAAATAAGAATGTGAGTATAAGATTTAAATTTGTTTTTATTATATTAGAACCTCCCTATACGATGGGTATTTTGTTCAACGGGATATTTTGTTACTGCATGCTCAGATGCTATT

Intron 3 GTAATTTGAT ... AATTGAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.13	158	46	24
A2	22.85	184	72	37
A3	19.02	166	38	40
A4	20.62	102	29	24
A5	19.81	83	16	25
A6	21.46	258	67	74
A7	19.37	385	99	86
A8	20.02	185.8	50	43
A9	22.71	272.3	73	87
A10	16.75	328	76	56

>TRINITY_DN112712_c1_g1_i1 from sample A2 / Example spliced isoform

TTTGAGAGAGTTTCCTCTGTTCTGCCCTCTATAATTCATTGAGCCGAGGATTTTAGGTTCTCAGTTAATTGAATTTTGTTCATTGAATTTAGTTGGTAGAATGGAGGCCGCCATGTCTGCATGCATTGCAGCAATGGCTCCCTGTATAAGTAACGAATCTAGAGGTATCAGTACTAATTATGTTACAGTTGGTTTAGGTC
GGGTTTATTTATCGTCAATTCGACAAACTGCTTCCCGGCTTGTGTTTTTTGATCGGAATCGCGTCCAAGCTTATGGTGGAACGGCTCAGCCTTATTGTGCGATCAAAAATAAATTAGAGAAGAAGAAATCAAATCATCTCTCTGCTGTGTCTGATGGGTGTTTATCAGAAAAAAAGAGGATAAAGCTATTACAATGGTTA
TCCAAAGATCTTTCTGTGCTGTCTGAGATCCCGGCAGATGCGAATGTATCCATTACAGAGCAAATCAGAGGAGAAATTCTCTCGGATGCTGTGAACGTGCTCCTGCGGCAATTAGAACAAACCAAAGCTGAGCGCAAGGAGAGGAAAAAGCGGTTGAAAGCCCAGAAAAAGGCTTTGAAATTAGCTGAAAAGCAGAGCAA
AAACAACGGCTGTTGTCAAGGCTCGTTGTCCTCATCATGCTCGTCCGACCCTGAGAGCCGGGTGGTGGGTATGGCTCTCCTCCACCCGACACAGCAAGTCGGACGGATTTTAGAGGCCTCTGAGCAAAATCCATTGCTGCTGATAGAAAGTCCTCCAAAAATAGCGGAGGTTAATGGAGATGAACAGGCTCTGCATGTGG
AATTTGCAGAGGCTAGTGATAGATTGGGGTTGGATTCAGGGGTGGGCATTCCGATGCAGGAGGCAAACAATGTGATAAAAGTTTGTATGGGTGGCAAGTGCAAGAAGTCGGGATCTGAGATGTTGTTGGAGGCGTTTGAAGAGAGAATAAGTAAAGCGGGGATGGGTTGTGAGGTGGAGGCGGTGGGATGCAAGTGCATG
GGTAAGTGCCGAAATGCTCCCAATATTCGAGTGCTGCAAACAGAGGATCAAGATGGCTTTCATGGCGGCAAGGGAGTACTGCACACGGGCGTAGACATTGGGGATGTCGATCTGATTCTTGCTCAGCACTTCTCGCAACAACCCCAACCACCAAAAGAAGGCCAAACAAATGGTTGTATATATTAATATCCCTCATCTCT
AAATTGGAGATCAGGGAAGCCCGATGATTAGGGGCCTCGGGGATTGTACAGTGATTCCCAGAGACTGTAAATATGTTTGTTTTGTGCTCTGATTATTCTGATTCTTTCAAAAGTCAAGCAATTTGTTGGCATGGAAGATTATCAGTAATTCGCTCAGATGGTAAAACAGTTTGGTTTTTCAGAATTGCTCATATAAAATG
AAATTGTGGAGTTCAGTTATCGCAGGAAAAGCGTTCTGTAAATATGTTAATGGAATCCAGAGAAGCTTTCTGTTTGAATTCAACGCTTTTGCAGTTTTCT

>TRINITY_DN112712_c1_g1_i3 from sample A2 / Example unspliced isoform:

TTTGAGAGAGTTTCCTCTGTTCTGCCCTCTATAATTCATTGAGCCGAGGATTTTAGGTTCTCAGTTAATTGAATTTTGTTCATTGAATTTAGTTGGTAGAATGGAGGCCGCCATGTCTGCATGCATTGCAGCAATGGCTCCCTGTATAAGTAACGAATCTAGAGGTATCAGTACTAATTATGTTACAGTTGGTTTAGGTC
GGGTTTATTTATCGTCAATTCGACAAACTGCTTCCCGGCTTGTGTTTTTTGATCGGAATCGCGTCCAAGCTTATGGTGGAACGGCTCAGCCTTATTGTGCGATCAAAAATAAATTAGAGAAGAAGAAATCAAATCATCTCTCTGCTGTGTCTGATGGGTGTTTATCAGAAAAAAAGAGGATAAAGCTATTACAATGGTTA
TCCAAAGATCTTTCTGTGCTGTCTGAGATCCCGGCAGATGCGAATGTATCCATTACAGAGCAAATCAGAGGAGAAATTCTCTCGgtaatttgattctttttcatcttcttgaaatattaatcttccactaaaataattcattatgatgaatggtattagatttactaaaaaccaatatgatcgagaattgaacagGATGC
TGTGAACGTGCTCCTGCGGCAATTAGAACAAACCAAAGCTGAGCGCAAGGAGAGGAAAAAGCGGTTGAAAGCCCAGAAAAAGGCTTTGAAATTAGCTGAAAAGCAGAGCAAAAACAACGGCTGTTGTCAAGGCTCGTTGTCCTCATCATGCTCGTCCGACCCTGAGAGCCGGGTGGTGGGTATGGCTCTCCTCCACCCGA
CACAGCAAGTCGGACGGATTTTAGAGGCCTCTGAGCAAAATCCATTGCTGCTGATAGAAAGTCCTCCAAAAATAGCGGAGGTTAATGGAGATGAACAGGCTCTGCATGTGGAATTTGCAGAGGCTAGTGATAGATTGGGGTTGGATTCAGGGGTGGGCATTCCGATGCAGGAGGCAAACAATGTGATAAAAGTTTGTATG
GGTGGCAAGTGCAAGAAGTCGGGATCTGAGATGTTGTTGGAGGCGTTTGAAGAGAGAATAAGTAAAGCGGGGATGGGTTGTGAGGTGGAGGCGGTGGGATGCAAGTGCATGGGTAAGTGCCGAAATGCTCCCAATATTCGAGTGCTGCAAACAGAGGATCAAGATGGCTTTCATGGCGGCAAGGGAGTACTGCACACGGG
CGTAGACATTGGGGATGTCGATCTGATTCTTGCTCAGCACTTCTCGCAACAACCCCAACCACCAAAAGAAGGCCAAACAAATGGTTGTATATATTAATATCCCTCATCTCTAAATTGGAGATCAGGGAAGCCCGATGATTAGGGGCCTCGGGGATTGTACAGTGATTCCCAGAGACTGTAAATATGTTTGTTTTGTGCTC
TGATTATTCTGATTCTTTCAAAAGTCAAGCAATTTGTTGGCATGGAAGATTATCAGTAATTCGCTCAGATGGTAAAACAGTTTGGTTTTTCAGAATTGCTCATATAAAATGAAATTGTGGAGTTCAGTTATCGCAGGAAAAGCGTTCTGTAAATATGTTAATGGAATCCAGAGAAGCTTTCTGTTTGAATTCAACGCTTT
TGCAGTTTTCT