sp|Q9LU39|GLUBP_ARATH ----> Glutamyl-tRNA reductase-binding protein, chloroplastic

Intron 1 GTAAGTCAGC ... ACTGAAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.07	22	47	25
A2	70.18	17	65	15
A3	69.23	20	61	29
A4	49.37	28.4	32	23.4
A5	88.89	2	17	15
A6	57.76	68	76	110
A7	62.72	74	118	131
A8	64.29	40	39	105
A9	62.39	63	99	110
A10	71.83	40	123	81

>TRINITY_DN95800_c0_g1_i4 from sample A4 / Example spliced isoform

CGGTCTTTGAGTAAATCTCACCGCACGAAGTACAACTATTTGCACATCTGAATACAGCGAATCCCAACACGCCATCGTCCTAGCCTTCCGCCCATTAATATTTTATTAAGGCCATCTGCTGACGGGAGACGGGAATGGCTTCTTAAGCTTACAGGGGAATGAAAGGTTGAACGAGGAGCAAACGAAGGTTCAATCAATAT
GGGCAAGGAGATTTGGTGAAGTAAATGAAGATCATTTATATATAATTGCTGTGGAAAAATTGCTTCAATCACAAGGCTTGGGCAAGGAGGGTTTTTGGGTTGATGCTGTGGAGTATTCAGGAGCAAGTGCCGATCCACTTCGTGATCGTGTAGCAAAGATAGTTAAAGATATGAATGAAAAACACTGGGAAGATATTCAT
CGCATATGCAATGTATATGCTGATTTGGATGTTGAGGTTGAGGAAGTGAGCATGATATGGATCGATAGATTAGGATTTGACTTGCACCTTCTCACCCATCCACAGAAAGAGATACTGGAGGTTCGGATTCCATTTCCAAGAGAAGTCACAGATGAAAGGGATGCCAGATCTTCGT

>TRINITY_DN95800_c0_g1_i5 from sample A4 / Example unspliced isoform:

CGGTCTTTGAGTAAATCTCACCGCACGAAGTACAACTATTTGCACATCTGAATACAGCGAATCCCAACACGCCATCGTCCTAGCCTTCCGCCCATTAATATTTTATTAAGGCCATCTGCTGACGGGAGACGGGAATGGCTTCTTAAGCTTACAGGGGAATGAAAGGTTGAACGAGGAGCAAACGgtaagtcagccatgta
ccttttgtcactttcatgacaggagatggtaacaggaatctaagctgaagaatacccataaacgaaagcattagaatgctgcctacgactatcctatccgcatttttattataaacatttttatggaaatatttctggtctagttgtagttgttgttgttgtatatatacaaatcttgccaccactcaattccagaattt
ctttgaggataagctctcggatctttatacaagagtgcccattagctaaaatccgagtaaaagggcccctcactttgagggaaaaatccagtacccattaaatcgaattgctgcaatctgccttacaaactgtccaaatctattgaaaaagagtgcccaagtgttcaaaaccattgaaaaatagtacccaagtgttcaaa
acacagaaatttctcaatggctcatccacttaggcacaattctatccccattacttacaatcttcctcctattcgtctctgcaaaaatgcaattactacacccaaagttttatcttttcgtcgcttcgccaatataccgtccatacgtatacgctggtctggatttaagacaaatgcacgttgtattactgggagttcaa
gggctccaaaatgtaatgtttcaattggtaaagttgagagcgtttcagatgggaaaatggcgttgcaaatcaggcctccaccagcagaaacttgtaggacactgatggcgctgtgctcagaaggcactctttgtacattgactcaagatgggtggccttttggcgacgctgttgagtttacagttgatgttcgagggatg
cctgtgctctgcttggaccctttgtcaatgcattctcagcatgtattgcaccatagattctgcagtctgcatgtgaagttggagcagcctggctgtagaaagtcacaatgcactctgatgggcactctttcgaagccagaagacagaatactgaagcagAAGGTTCAATCAATATGGGCAAGGAGATTTGGTGAAGTAAA
TGAAGATCATTTATATATAATTGCTGTGGAAAAATTGCTTCAATCACAAGGCTTGGGCAAGGAGGGTTTTTGGGTTGATGCTGTGGAGTATTCAGGAGCAAGTGCCGATCCACTTCGTGATCGTGTAGCAAAGATAGTTAAAGATATGAATGAAAAACACTGGGAAGATATTCATCGCATATGCAATGTATATGCTGATT
TGGATGTTGAGGTTGAGGAAGTGAGCATGATATGGATCGATAGATTAGGATTTGACTTGCACCTTCTCACCCATCCACAGAAAGAGATACTGGAGGTTCGGATTCCATTTCCAAGAGAAGTCACAGATGAAAGGGATGCCAGATCTTCGTTGACATACATGGCACAGGTAGCTTGGGAAGTTGAGAAAAACTATGCGCCT
CCAGAATTTGAAAGGGATGCCAGATCTTCGTTGACATACATGGCACAGGTAGCTTGGGAAGTTGAGAAAAACTATGCGCCTCCAGAATTTGAGAGGGTAAAGTGCATCCAGAAATAAAGGAGGTGACAGACTCTAGAATCAGCGTGTATTTGCTGAAATATAGTAGATAACCCTGCTTTCATCATTGCAAGTGTGGTAGT
TCATCTTCATTTTTTGGTGTGGTGGTGAACGAGTATTAAATGCTTATTCTTCCATTTGATAGGAACTTTTTAAATCCGTACGGCCAAGCATGCATGCTTATATGTTTACGGGAGAGTTCATTTTATACATGGATGGGAGAGGTTTTTTTGTTTATATACCTATAACATCTAAGTGAAATTTGTTTATGCTTTCTGAGCTA
AAACAATTATGCATATAAAACCTGTCTCTTGTTTGGTTGTAAACAGTACGCAGC

Intron 2 GTAAGTCAGC ... GCATGTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76	9	47	10
A2	79.55	9	65	5
A3	73.68	15	61	23
A4	60.73	14.6	32	13
A5	84.24	3.3	17	18
A6	67.27	36	76	72
A7	69.01	45.6	118	85
A8	69.53	19.3	39	49
A9	66.54	48.3	99	93
A10	79.08	28.6	123	93

>TRINITY_DN100364_c0_g1_i1 from sample A3 / Example spliced isoform

CGAAGTACAACTATTTGCACATCTGAATACAGCGAATCCCAACACGCCATCGTCCTAGCCTTCCGCCCATTAATATTTTATTAAGGCCATCTGCTGACGGGAGACGGGAATGGCTTCTTAAGCTTACAGGGGAATGAAAGGTTGAACGAGGAGCAAACGTTGGAGCAGCCTGGCTGTAGAAAGTCACAATGCACTCTGAT
GGGCACTCTTTCGAAGCCAGAAGACAGAATACTGAAGCAGGTTCAATCAATATGGGCAAGGAGATTTGGTGAAGTAAATGAAGATCATTTATATATAATTGCTGTGGAAAAATTGCTTCAATCACAAGGCTTGGGCAAGGAGGGTTTTTGGGTTGATGCTGTGGAGTATTCAGGAGCAAGTGCCGATCCACTTCGTGATC
GTGTAGCAAAGATAGTTAAAGATATGAATGAAAAACACTGGGAAGATATTCATCGCATATGCAATGTATATGCTGATTTGGATGTTGAGGTTGAGGAAGTGAGCATGATATGGATCGATAGATTAGGATTTGACTTGCACCTTCTCACCCATCCACAGAAAGAGATACTGGAGGTTCGGATTCCATTTCCAAGAGAAGTC
ACAGATGAAAGGGATGCCAGATCTTCGTTGACATACATGGCACAGGTAGCTTGGGAAGTTGAGAAAAACTATGCGCCTCCAGAATTTGAGAGGGTAAAGTGCATCCAGAAATAAAGGAGGTGACAGACTCTAGAATCAGCGTGTATTTGCTGAAATATAGTAGATAACCCTGCTTTCATCATTGCAAGTGTGGTAGTTCA
TCTTCATTTTTTGGTGTGGTGGTGAACGAGTATTAAATGCTTATTCTTCCATTTGATAGGAACTTTTTAAATCCGTACGGCCAAGCATGCATGCTTATATGTTTACGGGAGAGTTCATTTTATACATGGATGGGAGAGGTTTTTTTGTTTATATACCTATAACATCTAAGTGAAATTTGTTTATGCTTTCTGAGCTAAAA
CAATTATGCATATAAAACCTGTCTCTTGTTTGGTTGTAAACAGTAC

>TRINITY_DN100364_c0_g1_i2 from sample A3 / Example unspliced isoform:

CGAAGTACAACTATTTGCACATCTGAATACAGCGAATCCCAACACGCCATCGTCCTAGCCTTCCGCCCATTAATATTTTATTAAGGCCATCTGCTGACGGGAGACGGGAATGGCTTCTTAAGCTTACAGGGGAATGAAAGGTTGAACGAGGAGCAAACGgtaagtcagccatgtaccttttgtcactttcatgacaggag
atggtaacaggaatctaagctgaagaatacccataaacgaaagcattagaatgctgcctacgactatcctatccgcatttttattataaacatttttatggaaatatttctggtctagttgtagttgttgttgttgtatatatacaaatcttgccaccactcaattccagaatttctttgaggataagctctcggatctt
tatacaagagtgcccattagctaaaatccgagtaaaagggcccctcactttgagggaaaaatccagtacccattaaatcgaattgctgcaatctgccttacaaactgtccaaatctattgaaaaagagtgcccaagtgttcaaaaccattgaaaaatagtacccaagtgttcaaaacacagaaatttctcaatggctcat
ccacttaggcacaattctatccccattacttacaatcttcctcctattcgtctctgcaaaaatgcaattactacacccaaagttttatcttttcgtcgcttcgccaatataccgtccatacgtatacgctggtctggatttaagacaaatgcacgttgtattactgggagttcaagggctccaaaatgtaatgtttcaat
tggtaaagttgagagcgtttcagatgggaaaatggcgttgcaaatcaggcctccaccagcagaaacttgtaggacactgatggcgctgtgctcagaaggcactctttgtacattgactcaagatgggtggccttttggcgacgctgttgagtttacagttgatgttcgagggatgcctgtgctctgcttggaccctttgt
caatgcattctcagcatgtattgcaccatagattctgcagtctgcatgtgaagTTGGAGCAGCCTGGCTGTAGAAAGTCACAATGCACTCTGATGGGCACTCTTTCGAAGCCAGAAGACAGAATACTGAAGCAGAAGGTTCAATCAATATGGGCAAGGAGATTTGGTGAAGTAAATGAAGATCATTTATATATAATTGCT
GTGGAAAAATTGCTTCAATCACAAGGCTTGGGCAAGGAGGGTTTTTGGGTTGATGCTGTGGAGTATTCAGGAGCAAGTGCCGATCCACTTCGTGATCGTGTAGCAAAGATAGTTAAAGATATGAATGAAAAACACTGGGAAGATATTCATCGCATATGCAATGTATATGCTGATTTGGATGTTGAGGTTGAGGAAGTGAG
CATGATATGGATCGATAGATTAGGATTTGACTTGCACCTTCTCACCCATCCACAGAAAGAGATACTGGAGGTTCGGATTCCATTTCCAAGAGAAGTCACAGATGAAAGGGATGCCAGATCTTCGTTGACATACATGGCACAGGTAGCTTGGGAAGTTGAGAAAAACTATGCGCCTCCAGAATTTGAGAGGGTAAAGTGCA
TCCAGAAATAAAGGAGGTGACAGACTCTAGAATCAGCGTGTATTTGCTGAAATATAGTAGATAACCCTGCTTTCATCATTGCAAGTGTGGTAGTTCATCTTCATTTTTTGGTGTGGTGGTGAACGAGTATTAAATGCTTATTCTTCCATTTGATAGGAACTTTTTAAATCCGTACGGCCAAGCATGCATGCTTATATGTT
TACGGGAGAGTTCATTTTATACATGGATGGGAGAGGTTTTTTTGTTTATATACCTATAACATCTAAGTGAAATTTGTTTATGCTTTCTGAGCTAAAACAATTATGCATATAAAACCTGTCTCTTGTTTGGTTGTAAACAGTAC

Intron 3 GTAAGTCAGC ... AGCGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.17	11	47	13
A2	80.65	9	65	10
A3	77.14	12	61	20
A4	69.77	13	32	28
A5	73.33	4	17	5
A6	58.19	37	76	27
A7	68.89	42	118	68
A8	65.52	20	39	37
A9	68.56	36	99	58
A10	75.67	32	123	76

>TRINITY_DN131964_c1_g3_i3 from sample A1 / Example spliced isoform

GTACAACTATTTGCACATCTGAATACAGCGAATCCCAACACGCCATCGTCCTAGCCTTCCGCCCATTAATATTTTATTAAGGCCATCTGCTGACGGGAGACGGGAATGGCTTCTTAAGCTTACAGGGGAATGAAAGGTTGAACGAGGAGCAAACGATGGGAAAATGGCGTTGCAAATCAGGCCTCCACCAGCAGAAACTT
GTAGGACACTGATGGCGCTGTGCTCAGAAGGCACTCTTTGTACATTGACTCAAGATGGGTGGCCTTTTGGCGACGCTGTTGAGTTTACAGTTGATGTTCGAGGGATGCCTGTGCTCTGCTTGGACCCTTTGTCAATGCATTCTCAGCATGTATTGCACCATAGATTCTGCAGTCTGCATGTGAAGGTATGTATACTGGGC

>TRINITY_DN131964_c1_g3_i2 from sample A1 / Example unspliced isoform:

GTACAACTATTTGCACATCTGAATACAGCGAATCCCAACACGCCATCGTCCTAGCCTTCCGCCCATTAATATTTTATTAAGGCCATCTGCTGACGGGAGACGGGAATGGCTTCTTAAGCTTACAGGGGAATGAAAGGTTGAACGAGGAGCAAACGgtaagtcagccatgtaccttttgtcactttcatgacaggagatgg
taacaggaatctaagctgaagaatacccataaacgaaagcattagaatgctgcctacgactatcctatccgcatttttattataaacatttttatggaaatatttctggtctagttgtagttgttgttgttgtatatatacaaatcttgccaccactcaattccagaatttctttgaggataagctctcggatctttata
caagagtgcccattagctaaaatccgagtaaaagggcccctcactttgagggaaaaatccagtacccattaaatcgaattgctgcaatctgccttacaaactgtccaaatctattgaaaaagagtgcccaagtgttcaaaaccattgaaaaatagtacccaagtgttcaaaacacagaaatttctcaatggctcatccac
ttaggcacaattctatccccattacttacaatcttcctcctattcgtctctgcaaaaatgcaattactacacccaaagttttatcttttcgtcgcttcgccaatataccgtccatacgtatacgctggtctggatttaagacaaatgcacgttgtattactgggagttcaagggctccaaaatgtaatgtttcaattggt
aaagttgagagcgtttcagATGGGAAAATGGCGTTGCAAATCAGGCCTCCACCAGCAGAAACTTGTAGGACACTGATGGCGCTGTGCTCAGAAGGCACTCTTTGTACATTGACTCAAGATGGGTGGCCTTTTGGCGACGCTGTTGAGTTTACAGTTGATGTTCGAGGGATGCCTGTGCTCTGCTTGGACCCTTTGTCAAT
GCATTCTCAGCATGTATTGCACCATAGATTCTGCAGTCTGCATGTGAAGTTGGAGCAGCCTGGCTGTAGAAAGTCACAATGCACTCTGATGGGCACTCTTTCGAAGCCAGAAGACAGAATACTGAAGCAGAAGGTTCAATCAATATGGGCAAGGAGATTTGGTGAAGTAAATTAAGATCATTTATATATAATTGCTGTGG
AAAAATTGCTTCAATCACAAGGCTTGGGCAAGGAGGGTTTTTGGGTTGATGCTGTGGAGTATTCAGGAGCAAGTGCCGATCCACTTCGTGATCGTGTAGCAAAGATAGTTAAAGATATGAATGAAAAACACTGGGAAGATATTCATCGCATATGCAATGTATATGCTGATTTGGATGTTGAGGTTGAGGAAGTGAGCATG
ATATGGATCGATAGATTAGGATTTGACTTGCACCTTCTCACCCATCCACAGAAAGAGATACTGGAGGTTCGGATTCCATTTCCAAGAGAAGTCACAGATGAAAGGGATGCCAGATCTTCGTTGACATACATGGCACAGGTAGCTTGGGAAGTTGAGAAAAACTATGCGCCTCCAGAATTTGAGAGGGTAAAGTGCATCCA
GAAATAAAGGAGGTGACAGACTCTAGAATCAGCGTGTATTTGCTGAAATATAGTAGATAACCCTGCTTTCATCATTGCAAGTGTGGTAGTTCATCTTCATTTTTTGGTGTGGTGGTGAACGAGTATTAAATGCTTATTCTTCCATTTGATAGGAACTTTTTAAATCCGTACGGCCAAGCATGCATGCTTATATGTTTACG
GGAGAGTTCATTTTATACATGGATGGGAGAGGTTTTTTTGTTTATATACCTATAACATCTAAGTGAAATTTGTTTATGCTTTCTGAGCTAAAACAATTATGCATATAAAACCTGTCTCTTGTTTGGTTGTAAACAGTAA