sp|O22907|RH24_ARATH ----> DEAD-box ATP-dependent RNA helicase 24

Intron 1 GTTAGAAATG ... TGAACTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.45	24	20	8
A2	38.64	27	17	10
A3	42.5	23	17	5
A4	38.46	16	10	4
A5	41.86	25	18	5
A6	48.53	35	33	7
A7	44.09	52	41	4
A8	48.08	54	50	6
A9	50	39	39	1
A10	38.94	69	44	7

>TRINITY_DN108479_c0_g1_i6 from sample A2 / Example spliced isoform

CCCGAATATCAATGCAATTATTATCAACTGCACCATCAAAAAAATCTATATATAGTTATAGGAATTTTCTTACGTATCGCCGCCGGACAAATTTCCTGGATGAAATTTGTCGTTCCTGTATATTGGATTACGGAGTTGGACTCGTTGCCATTGAAACCGTGCCAGATTTTTGGTTCAGATGGTATATATAAGCTAGGGTT
TTCTGTCTTCAGAGAAATCTGAAGAATGTACTTGAAGAATTTCAGGGCTTTGGGTTTTTAAGAGAATTTTGAAGAATTTATGGGAAGGATCCGAGGGCTTTGGTTTTTTTTCAGAGAAATCTGAATAGCCTATTGGAAGAGGTTGAGATTTTTTTGCGGATTTTGTTTTCGGAAAAAACCCTCAAAATGTCGAAGCGAAA
GTTCGGCTTCGAGGGCTTCGGCATAAACAAGCAGACCAGTTACAACTTCGAGCCCGCGAAGGCTCCCCGTCGCGGACTCTACATCCCCAGTCACGACAACTGCGAAGATCACGACCTCGATGACATCGATTACGCAGATTCAAAAGAACCCCTGGTCGAGGGTTCGAACCTCTCGGAGCCCTCTGACAACAAGAACAACG
GAGAAGAAGAGGAAATTGACCCCCTGGATGCCTTCATGGCGGGTATTCAGGAGGAAATGAACAGCGTCCCGGCTAAGCCCAAGGCTAAGATGGAGAAATTTGAGGACGATGAAGATGATCCCGTGGAGAGCTTTCTCAAGGCTCGCAAGGACGCAGGATTGGCCCTGGCGGCTGAAGCCCTTCAGGCCGGTTATGACTCT
GACCAGGAGGTTTATGCCGCTGCCAAGGCCGTGGACGCCGGGCTGCTCGAGTACGATTCCGATGATAACCCCATTGTTCTTGACAAGAAGAAGATCGAGCCTATTCCGGCGCTGGATCATTCGGAGATCGATTATGAGCCGTTTAACAAGGATTTTTACGAGGAAAACCCTTCAATATCTGGTATGAGCGATCAGGAAGT
TACGGCATACCAGCAGAGTCTTGGAATCAAGGTTTCTGGATTTGATGTAACAAGGCCAATTAAGACATTTGAAGATTGTGGCTTTTCCTCTACATTGATGAATGCCATTGTTAAACAGGGTTATGTGAACCCAACCCCTATACAGTGCCAGGCTCTGCCAATTGTACTATCTGGAGGGGATATTATAGGAATAGCCAAGA
CTGGTTCAGGAAAAACTGTTGCATTTATTCTGCCAATGATTGTGCATATAATGGATCAGCCAGAACTTGCCAAAGAAGAAGGCCCAATTGGTGTTATTTGTGCACCTACAAGAGAATTGGCTCAGCAAATCTATATTGAGGCAAAGAAATTCGCGAAACCTCATGGCATCCGTGTTTCTGGTGTTTATGGAGGCATGTCA
AAGCTAGAACAGCTAAAAGAACTGAAGGCTGGTTGTGAGCTAGTGGTTGCTACCCCAGGAAGGTTGATTGACATGCTGAAGATGAAGGCGTTAACAATGCATAGAACAACTTACCTAGTGCTTGATGAGGCAGATAGGATGTTTGATCTGGGGTTTGAGCCTCAAATCAGATCTATTGTGGGTCAGATACGGCCAGATCG
CCAGACACTGCTCTTTTCTGCAACTATGCCAAGGAGAGTGGAACGCTTGGCAAGAGAAGTTCTGTCAAATCCTGTAAGGGTAACTGTAGGGGAAATAGGAAAGGCAAATGAGGACATCACACAAATTGTCCATGTTATCCCTTCAGATGCAGAGAAAATGCCTTGGCTTCTGAGCAAACTTCCTGCAATGGTTGATAATG
GAGATGTTCTAGTTTTTGCTTCGACCAAAGCAAAGGTTGTTGAGATTGAACCTCAGCTGATCCAAGATGGGTTCAAAGTTGCAACCCTTCATGGAGATAAGGATCAGTCTTCTCGTATGGAGGTTCTACAAAAGTTTAAGTCAGGGATTTACCATGTTCTTGTTGCAACTGATGTTGCAGCTCGTGGTCTTGATATCAAG
TCAATTAAATCTGTTGTTAACTTTGATATTGCCAGAGACATAGATATGCATGTTCACCGTATTGGAAGGACAGGGCGTGCAGGGGACAAAGAAGGCATAGCTTACACCCTTGTTACACAAAAACAAGCACACTTTGCAGGGGAACTAGTGAACAGTTTGGTTGCTGCTGGTCAAAATGTCTCCATGGAGCTAATGGATCT
TGCAATGAAGATTTGTCAAAAAAGTTCACTGAGAACTTTTTGTCTGGTGACAAAAATATGCAATTCACATTTCTCACCTGGATTTGTACTGCAACATTGTAGGATAGTCGTTTCCGGGCCAAACGGGAGGCCAGTAAAGGCTCTGGTATGTGATTCTC

>TRINITY_DN108479_c0_g1_i3 from sample A2 / Example unspliced isoform:

CCCGAATATCAATGCAATTATTATCAACTGCACCATCAAAAAAATCTATATATAGTTATAGGAATTTTCTTACGTATCGCCGCCGGACAAATTTCCTGGATGAAATTTGTCGTTCCTGTATATTGGATTACGGAGTTGGACTCGTTGCCATTGAAACCGTGCCAGATTTTTGGTTCAGATGGTATATATAAGCTAGGGTT
TTCTGTCTTCAGAGAAATCTGAAGAATGTACTTGAAGAATTTCAGGGCTTTGGGTTTTTAAGAGAATTTTGAAGAATTTATGGGAAGGATCCGAGGGCTTTGGTTTTTTTTCAGAGAAATCTGAATAGCCTATTGGAAGAGGTTGAGATTTTTTTGCGGATTTTGTTTTCGGAAAAAACCCTCAAAATGTCGAAGCGAAA
GTTCGGCTTCGAGGGCTTCGGCATAAACAAGCAGACCAGTTACAACTTCGAGCCCGCGAAGGCTCCCCGTCGCGGACTCTACATCCCCAGTCACGACAACTGCGAAGATCACGACCTCGATGACATCGATTACGCAGATTCAAAAGAACCCCTGGTCGAGGGTTCGAACCTCTCGGAGCCCTCTGACAACAAGAACAACG
GAGAAGAAGAGGAAATTGACCCCCTGGATGCCTTCATGGCGGGTATTCAGGAGGAAATGAACAGCGTCCCGGCTAAGCCCAAGGCTAAGATGGAGAAATTTGAGGACGATGAAGATGATCCCGTGGAGAGCTTTCTCAAGGCTCGCAAGGACGCAGGATTGGCCCTGGCGGCTGAAGCCCTTCAGGCCGGTTATGACTCT
GACCAGGAGGTTTATGCCGCTGCCAAGGCCGTGGACGCCGGGCTGCTCGAGTACGATTCCGATGATAACCCCATTGTTCTTGACAAGAAGAAGATCGAGCCTATTCCGGCGCTGGATCATTCGGAGATCGATTATGAGCCGTTTAACAAGGATTTTTACGAGGAAAACCCTTCAATATCTGGTATGAGCGATCAGGAAGT
TACGGCATACCAGCAGAGTCTTGGAATCAAGGTTTCTGGATTTGATGTAACAAGGCCAATTAAGACATTTGAAGATTGTGGCTTTTCCTCTACATTGATGAATGCCATTGTTAAACAGGGTTATGTGAACCCAACCCCTATACAGTGCCAGGCTCTGCCAATTGTACTATCTGGAGGGGATATTATAGGAATAGCCAAGA
CTGGTTCAGGAAAAACTGTTGCATTTATTCTGCCAATGATTGTGCATATAATGGATCAGCCAGAACTTGCCAAAGAAGAAGGCCCAATTGGTGTTATTTGTGCACCTACAAGAGAATTGGCTCAGCAAATCTATATTGAGGCAAAGAAATTCGCGAAACCTCATGGCATCCGTGTTTCTGGTGTTTATGGAGGCATGTCA
AAGCTAGAACAGCTAAAAGAACTGAAGGCTGGTTGTGAGCTAGTGGTTGCTACCCCAGGAAGGTTGATTGACATGCTGAAGATGAAGGCGTTAACAATGCATAGAACAACTTACCTAGTGCTTGATGAGGCAGATAGGATGTTTGATCTGGGGTTTGAGCCTCAAATCAGATCTATTGTGGGTCAGATACGGCCAGATCG
CCAGACACTGCTCTTTTCTGCAACTATGCCAAGGAGAGTGGAACGCTTGGCAAGAGAAGTTCTGTCAAATCCTGTAAGGGTAACTGTAGGGGAAATAGGAAAGGCAAATGAGGACATCACACAAATTGTCCATGTTATCCCTTCAGATGCAGAGAAAATGCCTTGGCTTCTGAGCAAACTTCCTGCAATGGTTGATAATG
GAGATGTTCTAGTTTTTGCTTCGACCAAAGCAAAGGTTGTTGAGATTGAACCTCAGCTGATCCAAGATGGGTTCAAAGTTGCAACCCTTCATGGAGATAAGGATCAGTCTTCTCGTATGGAGGTTCTACAAAAGTTTAAGTCAGGGATTTACCATGTTCTTGTTGCAACTGATGTTGCAGCTCGTGGTCTTGATATCAAG
TCAATTAAATCTGTTGTTAACTTTGATATTGCCAGAGACATAGATATGCATGTTCACCGTATTGGAAGGACAGGGCGTGCAGGGGACAAAGAAGGCATAGCTTACACCCTTGTTACACAAAAACAAGCACACTTTGCAGGGGAACTAGTGAACAGTTTGGTTGCTGCTGGTCAAAATGTCTCCATGGAGCTAATGGATCT
TGCAATGAAGgttagaaatgaaaatttgaaaactctgactgtactggttaatttttgtatgattttgttgttgaagttattaagctctttggtttacatttgaactacagATTTGTCAAAAAAGTTCACTGAGAACTTTTTGTCTGGTGACAAAAATATGCAATTCACATTTCTCACCTGGATTTGTACTGCAACATTGT
AGGATAGTCGTTTCCGGGCCAAACGGGAGGCCAGTAAAGGCTCTGGTATGTGATTCTC

Intron 2 GTTAGAAATG ... AACATTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.26	42	20	7
A2	43.59	22	17	10
A3	34.69	32	17	4
A4	37.04	17	10	3
A5	45	22	18	6.5
A6	34.38	63	33	6
A7	28.28	104	41	5
A8	40.98	72	50	6
A9	30.71	88	39	1
A10	34.11	85	44	8

>TRINITY_DN114829_c0_g1_i1 from sample A1 / Example spliced isoform

GAGTTTTGCTCATTTTATTCCTGATTAACTCATGTCCTCTTGCTGGTATTTGGTATGATGTTGATTCAGGTATGAGCGATCAGGAAGTTACGGCATACCAGCAGAGTCTTGGAATCAAGGTTTCTGGATTTGATGTAACAAGGCCAATTAAGACATTTGAAGATTGTGGCTTTTCCTCTACATTGATGAATGCCATTGTT
AAACAGGGTTATGTGAACCCAACCCCTATACAGTGCCAGGCTCTGCCAATTGTACTATCTGGAGGGGATATTATAGGAATAGCCAAGACTGGTTCAGGAAAAACTGTTGCATTTATTCTGCCAATGATTGTGCATATAATGGATCAGCCAGAACTTGCCAAAGAAGAAGGCCCAATTGGTGTTATTTGTGCACCTACAAG
AGAATTGGCTCAGCAAATCTATATTGAGGCAAAGAAATTCGCGAAACCTCATGGCATCCGTGTTTCTGGTGTTTATGGAGGCATGTCAAAGCTCGAACAGCTAAAAGAACTGAAGGCTGGTTGTGAGCTAGTGGTTGCTACCCCAGGAAGGTTGATTGACATGCTGAAGATGAAGGCGTTAACAATGCATAGAACAACTT
ACCTAGTGCTTGATGAGGCAGATAGGATGTTTGATCTGGGGTTTGAGCCTCAAATCAGATCTATTGTGGGTCAGATACGGCCAGATCGCCAGACACTGCTCTTTTCTGCAACTATGCCAAGGAGAGTGGAACGCTTGGCAAGAGAAGTTCTGTCAAATCCTGTAAGGGTAACTGTAGGGGAAATAGGAAAGGCAAATGAG
GACATCACACAAATTGTCCATGTTATCCCTTCAGATGCAGAGAAAATGCCTTGGCTTCTGAGCAAACTTCCTGCAATGGTTGATAATGGAGATGTTCTAGTTTTTGCTTCGACCAAAGCAAAGGTTGTTGAGATTGAACCTCAGCTGATCCAAGACGGGTTCAAAGTTGCAACCCTTCATGGAGATAAGGATCAGTCTTC
TCGTATGGAGGTTCTACAAAAGTTTAAGTCAGGGATTTACCATGTTCTTGTTGCAACTGATGTTGCAGCTCGTGGTCTTGATATCAAGTCAATTAAATCTGTTGTTAACTTTGATATTGCCAGAGACATAGATATGCATGTTCACCGTATTGGAAGGACAGGGCGTGCGGGGGACAAAGAAGGCATAGCTTACACCCTTG
TTACACAAAAACAAGCACACTTTGCAGGGGAACTAGTGAACAGTTTGGTTGCTGCTGGTCAAAATGTCTCCATGGAGCTAATGGATCTTGCAATGAAGGATAGTCGTTTCCGGGCCAAACGGGAGGCCAGTAAAGGCTCTGGTAAGAGGAAAGG

>TRINITY_DN114829_c0_g1_i3 from sample A1 / Example unspliced isoform:

GTTATAGGAATTTTCTTACGTATCGCCGCCGGACAAATTTCCTGGATGAAATTTGTCGTTCCTGTATATTGGATTACGGAGTTGGACTCGTTGCCATTGAAACCGTGCCAGATTTTTGGTTCAGATGGTATATATAAGCTAGGGTTTTCTGTCTTCAGAGAAATCTGAAGAATGTACTTGAAGAATTTCAGGGCTTTGGG
TTTTTAAGAGAATTTTGAAGAATTTATGGGAAGGATCCGAGGGCTTTGGTTTTTTTTCAGAGAAATCTGAATAGCCTATTGGAAGAGGTTGAGATTTTTTTGCGGATTTTGTTTTCGGAAAAAACCCTCAAAATGTCGAAGCGAAAGTTCGGCTTCGAGGGCTTCGGCATAAACAAGCAGACCAGTTACAACTTCGAGCC
CGCGAAGGCTCCCCGTCGCGGACTCTACATCCCCAGTCACGACAACTGCGAAGATCACGACCTCGATGACATCGATTACGCAGATTCAAAAGAACCCCTGGTCGAGGGTTCGAACCTCTCGGAGCCCTCTGACAACAAGAACAACGGAGAAGAAGAGGAAATTGACCCCCTGGATGCCTTCATGGCGGGTATTCAGGAGG
AAATGAACAGCGTCCCGGCTAAGCCCAAGGCTAAGATGGAGAAATTTGAGGACGATGAAGATGATCCCGTGGAGAGCTTTCTCAAGGCTCGCAAGGACGCAGGATTGGCCCTGGCGGCTGAAGCCCTTCAGGCCGGTTATGACTCTGACCAGGAGGTTTATGCCGCTGCCAAGGCCGTGGACGCCGGGCTGCTCGAGTAC
GATTCCGATGATAACCCCATTGTTCTTGACAAGAAGAAGATCGAGCCTATTCCGGCGCTGGATCATTCGGAGATCGATTATGAGCCGTTTAACAAGGATTTTTACGAGGAAAACCCTTCAATATCTGGTATGAGCGATCAGGAAGTTACGGCATACCAGCAGAGTCTTGGAATCAAGGTTTCTGGATTTGATGTAACAAG
GCCAATTAAGACATTTGAAGATTGTGGCTTTTCCTCTACATTGATGAATGCCATTGTTAAACAGGGTTATGTGAACCCAACCCCTATACAGTGCCAGGCTCTGCCAATTGTACTATCTGGAGGGGATATTATAGGAATAGCCAAGACTGGTTCAGGAAAAACTGTTGCATTTATTCTGCCAATGATTGTGCATATAATGG
ATCAGCCAGAACTTGCCAAAGAAGAAGGCCCAATTGGTGTTATTTGTGCACCTACAAGAGAATTGGCTCAGCAAATCTATATTGAGGCAAAGAAATTCGCGAAACCTCATGGCATCCGTGTTTCTGGTGTTTATGGAGGCATGTCAAAGCTCGAACAGCTAAAAGAACTGAAGGCTGGTTGTGAGCTAGTGGTTGCTACC
CCAGGAAGGTTGATTGACATGCTGAAGATGAAGGCGTTAACAATGCATAGAACAACTTACCTAGTGCTTGATGAGGCAGATAGGATGTTTGATCTGGGGTTTGAGCCTCAAATCAGATCTATTGTGGGTCAGATACGGCCAGATCGCCAGACACTGCTCTTTTCTGCAACTATGCCAAGGAGAGTGGAACGCTTGGCAAG
AGAAGTTCTGTCAAATCCTGTAAGGGTAACTGTAGGGGAAATAGGAAAGGCAAATGAGGACATCACACAAATTGTCCATGTTATCCCTTCAGATGCAGAGAAAATGCCTTGGCTTCTGAGCAAACTTCCTGCAATGGTTGATAATGGAGATGTTCTAGTTTTTGCTTCGACCAAAGCAAAGGTTGTTGAGATTGAACCTC
AGCTGATCCAAGACGGGTTCAAAGTTGCAACCCTTCATGGAGATAAGGATCAGTCTTCTCGTATGGAGGTTCTACAAAAGTTTAAGTCAGGGATTTACCATGTTCTTGTTGCAACTGATGTTGCAGCTCGTGGTCTTGATATCAAGTCAATTAAATCTGTTGTTAACTTTGATATTGCCAGAGACATAGATATGCATGTT
CACCGTATTGGAAGGACAGGGCGTGCGGGGGACAAAGAAGGCATAGCTTACACCCTTGTTACACAAAAACAAGCACACTTTGCAGGGGAACTAGTGAACAGTTTGGTTGCTGCTGGTCAAAATGTCTCCATGGAGCTAATGGATCTTGCAATGAAGgttagaaatgaaaatttgaaaactctgactgtactggttaattt
ttgtatgattttgttgttgaagttattaagctctttggtttacatttgaactacagatttgtcaaaaaagttcactgagaactttttgtctggtgacaaaaatatgcaattcacatttctcacctggatttgtactgcaacattgtagGATAGTCGTTTCCGGGCCAAACGGGAGGCCAGTAAAGGCTCTGGTAAGAGGA
AAGG