sp|Q8RWS4|BYST_ARATH ----> Bystin

Intron 1 GTTTGTGGGC ... CTTGAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.8	23	80.8	155.9
A2	89.34	25	82.8	214.8
A3	91.38	20	109.8	217.5
A4	84.26	40	61.5	220.3
A5	84.17	20	42.4	109
A6	83.7	44	70.6	234.1
A7	84.06	83	179.3	450.6
A8	84.46	35	69.3	196.8
A9	85.04	76	195.5	447.1
A10	86.16	69	167.6	440.4

>TRINITY_DN132819_c1_g2_i13 from sample A1 / Example spliced isoform

GCGCCACATTGCTGGCTTGCGTCCGTAGGTTTGCCATTACTTCACTCGTTTGGGTGGTTCGGCGGGCCATCACTTCATGTGGTAAGTTACTGCATGCGGCAAGTTCCTGTGCGGATGATTTCTTTTGCTGTATAGTTGGCGGTCTAAGAGGGAGCGTGTTCGAATCGCCGCATTTTCAAGAAGAAGTGTCATTCAATATG
GCTCCAGAGCACGCTTCGAATAATGGAGGGATGTCCTCAACCAAGAAAAAACGGAAGTCTCTAGGGAGTTCAGAAAAACATCAGTTTACATTTGAACAGGAAATGGATGCACAATTTGATAGACCTAGCAAGAAACGCCATGGCAAAGTTCGGAAGCAGCAGGAGAGTGAAGAGTATGTAAGTCAAACAATCAGCAATAA
GATATTGAGAGAGGCTCACAGGCAGCAAAAGGAAATTGATAATGAGAGGGATGAAGAAGCTGGTTTAGCATCTGCCAAAAAAGCGTTTTCTTTGGTTATAGAGGAGGCCAAATCTGACAGTGATGAGGATGACTTTGATGGGTTTTCTGAAACTCAAAGCCAGTATGAAGGGTATGAAGAGGTACCTGAGGACGATGAAA
AGGTATTGGCTGCATTTATGTCTGACAATGTTGGCCCTCAGCGTACTTTAGCTGATCTTATTATAGAGAGAATCCGAGAAAAAAATGCTGCAGTTTCTTCAGAAGAACGACCCCTTCCTTCACTGGATAATGGAATTATTGATGTTTATAAAGGAGTGGGTAAGCTGCTTAGTCGATACACTGCTGGTAAACTTCCAAAG
GCTTTGAAGGTTGTGCCATCATTGTCCCTCTGGGAGGAGGTGTTATATCTCGCAGAGCCAGAGAAATGGTCACCGAATGCAATGTACCAAGTAACTCGTATTTTTGTATCTAATCTAAATGCCAAGATGGCCCAGCGCTTCTGCATCCTTGTCCTACTTCCACGACTTAGGGAAGACATCAATAAAAATAAGCGACTTCA
TTTTGCATTGTACCAAGCCTTGAAAAAGTCATTGTACAAGCCACCGGCCTTCTTCAAAGGCATTCTCTTGCCTCTCTGTCAGTCACGTACTTGCAATCTGCGGGAAGCTGTCATTATTGGAAGTGTCATTCAGAAAGTCTCGATTCCTTCACTTCATTCTAGTGCTGGTCTAATGAAAATAGCAGAGATGGAGTACTGTG
GCACAAACAGTTACTTTATAAAGCTTCTTTTGGACAAAAAGTACGCTTTACCATATCGAGTGTTGGATGCTGTTTTTGAACACTTCAAGAGATTTTTTAATGAAACACGTATCATGCCGGTGATATGGCACCAATCCCTTCTGACTTTTGTCCAGAGATACAAGAATGAATTGACGAAGCAGCAGAAGGATGATTTGGAA
AACTTGGTTCAACATCAGAAGCATTACTTGGTGACCCCTGAAATCCTGAGAGAATTGAGGAACAGTCGAAATCGAGGCGAGAAAGAAAGCGACATTCTCATGTCTACTTTTTCTCCAGTTTCTGTTATTAACAAAACAATACAAGAAGATAGGTGGGATGTTCCAGAAGTTCCAATGGATGAAGACTAGTGGCCAAATAT
GATCATAGCTCGTTTTCAGGAGTACCTGTGGAGGCTACATTTTTTACTATTATATGCGATTTGTTTGCCTTGTAGACAAGCAAGTTCTAAAATATGGGCATATGATATTGATTACCAGTGTTATTTAATTCCGAAAAGGAGGGTCCTTTGACTAGTGATGTATCCTTGTGTGCGCATTGCCATTGTATCATAAAGTTTTG
ACGGGAAGAGTTGGGGCTGTTCAGTCTGATTTCAAAATAACTAGCATCATATGCAGATTTAATCGAGTATAATAAAAGAGCAATCAAATTTTATATTTTATATGCATGGAATGTGTTTTTGGAATTGGAATGGTCTTGGAAATTTAAATCTAGCCTTCTTCTATTATGGAGCATTATTCGATGTTTCCCCAGCCAATTTC
ACTGTCATATTACTCTCTTAATGTCAGATCAGTAGATTCTGAACATTTTTATTATTCTTGAATAGATTAATAAGTTTTGATCTTGTTATCACTTTACGATTGTAAATATATAAAGATGTAGAATTACCTCAG

>TRINITY_DN132819_c1_g2_i1 from sample A1 / Example unspliced isoform:

GTGGGCCACTACTGATAAAAAAAACGCTCAAGAATAGACAACCCTCCCCATATACAACCCTCCCCTAGTTGGCGGTCTAAGgtttgtgggcgccgttgtactcttcattgcatccctatagtcaaggttctcagaaaattgtaactgcgaattttctggtgtggacactgtttgtttttgtcatttcacttctctgtttt
gatcgatccatctacgctgctgcttgaatcagAGGGAGCGTGTTCGAATCGCCGCATTTTCAAGAAGAAGTGTCATTCAATATGGCTCCAGAGCACGCTTCGAATAATGGAGGGATGTCCTCAACCAAGAAAAAACGGAAGTCTCTAGGGAGTTCAGAAAAACATCAGTTTACATTTGAACAGGAAATGGATGCACAATT
TGATAGACCTAGCAAGAAACGCCATGGCAAAGTTCGGAAGCAGCAGGAGAGTGAAGAGTATGTAAGTCAAACAATCAGCAATAAGATATTGAGAGAGGCTCACAGGCAGCAAAAGGAAATTGATAATGAGAGGGATGAAGAAGCTGGTTTAGCATCTGCCAAAAAAGCGTTTTCTTTGGTTATAGAGGAGGCCAAATCTG
ACAGTGATGAGGATGACTTTGATGGGTTTTCTGAAACTCAAAGCCAGTATGAAGGGTATGAAGAGGTACCTGAGGACGATGAAAAGGTATTGGCTGCATTTATGTCTGACAATGTTGGCCCTCAGCGTACTTTAGCTGATCTTATTATAGAGAGAATCCGAGAAAAAAATGCTGCAGTTTCTTCAGAAGAACGACCCCTT
CCTTCACTGGATAATGGAATTATTGATGTTTATAAAGGAGTGGGTAAGCTGCTTAGTCGATACACTGCTGGTAAACTTCCAAAGGCTTTGAAGGTTGTGCCATCATTGTCCCTCTGGGAGGAGGTGTTATATCTCGCAGAGCCAGAGAAATGGTCACCGAATGCAATGTACCAAGTAACTCGTATTTTTGTATCTAATCT
AAATGCCAAGATGGCCCAGCGCTTCTGCATCCTTGTCCTACTTCCACGACTTAGGGAAGACATCAATAAAAATAAGCGACTTCATTTTGCATTGTACCAAGCCTTGAAAAAGTCATTGTACAAGCCACCGGCCTTCTTCAAAGGCATTCTCTTGCCTCTCTGTCAGTCACGTACTTGCAATCTGCGGGAAGCTGTCATTA
TTGGAAGTGTCATTCAGAAAGTCTCGATTCCTTCACTTCATTCTAGTGCTGGTCTAATGAAAATAGCAGAGATGGAGTACTGTGGCACAAACAGTTACTTTATAAAGCTTCTTTTGGACAAAAAGTACGCTTTACCATATCGAGTGTTGGATGCTGTTTTTGAACACTTCAAGAGATTTTTTAATGAAACACGTATCATG
CCGGTGATATGGCACCAATCCCTTCTGACTTTTGTCCAGAGATACAAGAATGAATTGACGAAGCAGCAGAAGGATGATTTGGAAAACTTGGTTCAACATCAGAAGCATTACTTGGTGACCCCTGAAATCCTGAGAGAATTGAGGAACAGTCGAAATCGAGGCGAGAAAGAAAGCGACATTCTCATGTCTACTTTTTCTCC
AGTTTCTGTTATTAACAAAACAATACAAGAAGATAGGTGGGATGTTCCAGAAGTTCCAATGGATGAAGACTAGTGGCCAAATATGATCATAGCTCGTTTTCAGGAGTACCTGTGGAGGCTACATTTTTTACTATTATATGCGATTTGTTTGCCTTGTAGACAAGCAAGTTCTAAAATATGGGCATATGATATTGATTACC
AGTGTTATTTAATTCCGAAAAGGAGGGTCCTTTGACTAGTGATGTATCCTTGTGTGCGCATTGCCATTGTATCATAAAGTTTTGACGGGAAGAGTTGGGGCTGTTCAGTCTGATTTCAAAATAACTAGCATCATATGCAGATTTAATCGAGTATAATAAAAGAGCAATCAAATTTTATATTTTATATGCATGGAATGTGT
TTTTGGAATTGGAATGGTCTTGGAAATTTAAATCTAGCCTTCTTCTATTATGGAGCATTATTCGATGTTTCCCCAGCCAATTTCACTGTCATATTACTCTCTTAATGTCAGATCAGTAGATTCTGAACATTTTTATTATTCTTGAATAGATTAATAAGTTTTGATCTTGTTATCACTTTACGATTGTAAATATATAAAGA
TGTAGAATTACCTCAG

Intron 2 GTAGGCCTCT ... TTTGCCAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.41	73	45	67
A2	39.89	55	32	41
A3	43.28	57	42	45
A4	42.5	46	29	39
A5	44.9	27	18	26
A6	33.33	11	7	4
A7	35	13	11	3
A8	27.27	8	5	1
A9	33.33	10	6	4
A10	58.14	9	8	17

>TRINITY_DN110903_c1_g1_i10 from sample A4 / Example spliced isoform

CCAAGACACTGTCCTTGATTCCACGTGGCTGGCTTGCTTTCTTCGGTTTTCTATTACTTCACACGTCTGGATGGTTCGGCCTGCCACCAAAGTTCCTGTGCGGAATTTCTCTCGGTTTGTAGTTGCCGCCGCCGTTCTAAGGTTTGTTGGCGTCCTTGTACTCTTCATTGCAGGGCCATATTCAAGGTTCTGAGAAAATT
GAGAAAATTGCAACTGCAAATTTTCTGGTGTGTGACACTGTTTTTGTCATTTCACTTCTCCGTCCTGAACGATCCATCCACGCATCTACGCTGCTGCTTCAATCAGAGGGAGCGTGTTCGAATCGCCGCATTTTCAAGAAGGTATCTTGCTTGACCGAGAAAATTGTAACTGCGAATTTTCTGGCGTGGACACTGTTTTT
GTCATTTAACTTCTCCGTCCTGAACGATCCATCCACGCATCTACGCTGCTGCTTCAATCAGAGGGAGCGTGTTCGAATCGCCGCATTTTCAAGAAG

>TRINITY_DN110903_c1_g1_i8 from sample A4 / Example unspliced isoform:

CCAAGACACTGTCCTTGATTCCACGTGGCTGGCTTGCTTTCTTCGGTTTTCTATTACTTCACACGTCTGGATGGTTCGGCCTGCCACCAAAGTTCCTGTGCGGAATTTCTCTCGGTTTGTAGTTGCCGCCGCCGTTCTAAGGTTTGTTGGCGTCCTTGTACTCTTCATTGCAGGGCCATATTCAAGGTTCTGAGAAAATT
GAGAAAATTGCAACTGCAAATTTTCTGGTGTGTGACACTGTTTTTGTCATTTCACTTCTCCGTCCTGAACGATCCATCCACGCATCTACGCTGCTGCTTCAATCAGAGGGAGCGTGTTCGAATCGCCGCATTTTCAAGAAGgtaggcctctggaaggaaccccattattttttcaaattacaatttcaatgcattttcgc
tgtttctagggttggttttgtgcacaggtttacttagggaatatggtgctatcacctcaaatggaaaagttaaacgaccccaaacgaatgggccttcgctcctgactttcttgatcttgctatccaacttgtcactgtttgcttgctttcccattgcccacatcatacgcaagagagaaggccccatcacaggagaaggc
ccccgttcacgagaccccgttaagtataagaaccctgttgattatactctgttcatcatccattcaacagccatatccgggaggagagagaaggactgtgaaacagtgaaaacacttcaagtctgcagtgaacctctgctgatcaatcaaacttgctgtttgccaaagGTATCTTGCTTGACCGAGAAAATTGTAACTGC
GAATTTTCTGGCGTGGACACTGTTTTTGTCATTTAACTTCTCCGTCCTGAACGATCCATCCACGCATCTACGCTGCTGCTTCAATCAGAGGGAGCGTGTTCGAATCGCCGCATTTTCAAGAAG

Intron 3 GTAAGTTACT ... CTTGAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	89.67	19	26	165
A2	90.76	23	15	226
A3	93.09	17	56	229
A4	86.94	35	14	233
A5	83.33	23	13	115
A6	83.84	48	10	249
A7	85.48	81	54	477
A8	85.66	35	20	209
A9	85.13	83	46	475
A10	87.71	65	64	464

>TRINITY_DN44969_c0_g3_i3 from sample A6 / Example spliced isoform

TAGATAAGCGCCACATTGCTGGCTTGCGTCCGTAGGTTTGCCATTACTTCACTCGTTTGGGTGGTTCGGCGGGCCATCACTTCATGTGAGGGAGCGTGTTCGAATCGCCGCATTTTCAAGAAGAAGTGTCATTCAATATGGCTCCAGAGCACGCTTCGAATAATGGAGGGATGTCCTCAACCAAGAAAAAACGGAAGTCT
CTAGGGAGTTCAGAAAAACATCAGTTTACATTTGAACAGGAAATGGATGCACAATTTGATAGACCTAGCAAGAAACGCCATGGCAAAGTTCGGAAGCAGCAGGAGAGTGAAGAGTATGTAAGTCAAACAATCAGCAATAAAATATTGAGAGAGGCTCACCGGCAGCAAAAGGAAATTGATAATGAGAGGGATGAAGAAGC
TGGTTTAGCATCTGCCAAAAAAGCGTTTTCTTTGGTTATAGAGGAGGCCAAATCTGACAGTGATGAGGATGACTTTGATGGGTTTTCTGAAACTCAAAGCCAGTATGAAGGGTATGAAGAGGTACCTGAGGACGATGAAAAGGTATTGGCTGCATTTATGTCTGACAATGTTGGCCCTCAACGTACTTTAGCTGATCTTA
TTATAGAGAGAATCCGAGAAAAAAATGCTGCAGTTTCTTCAGAAGAACGACCCCTTCCTTCACTGGATAATGGAATTATTGATGTTTATAAAGGAGTGGGTAAGCTGCTTAGTCGATACACTGCTGGTAAACTTCCAAAGGCTTTGAAGGTTGTGCCATCATTGTCCCTCTGGGAGGAGGTGTTATATCTCGCAGAGCCA
GAGAAATGGTCACCGAATGCAATGTACCAAGTAACTCGTATTTTTGTATCTAATCTAAATGCCAAGATGGCCCAGCGCTTCTGCATCCTTGTCCTACTTCCACGACTTAGGGAAGACATCAATAAAAATAAGCGACTTCATTTTGCATTGTACCAAGCCTTGAAAAAGTCATTGTACAAGCCACCGGCCTTCTTCAAAGG
CATTCTCTTGCCTCTCTGTCAGTCACGCACTTGCAATCTGCGGGAAGCTGTCATTATTGGAAGTGTCATTCAGAAAGTCTCGATTCCTTCACTTCATTCTAGTGCTGGTCTAATGAAAATAGCAGAGATGGAGTACTGTGGCACAAACAGTTACTTTATAAAGCTTCTTTTGGACAAAAAGTACGCTTTACCATATCGAG
TGTTGGATGCTGTTTTTGAACACTTCAAGAGATTTTTTAATGAAACACGTATCATGCCGGTGATATGGCACCAATCCCTTCTGACTTTTGTCCAGAGATACAAGAATGAATTGACGAAGCAGCAGAAGGATGATTTGGAAAACTTGGTTCAACATCAGAAGCATTACTTGGTGACCCCTGAAATCCTGAGAGAATTGAGG
AACAGTCGAAATCGAGGCGAGAAAGAAAGCGACATTCTCATGTCTACTTTTTCTCCAGTTTCTGTTATTAACAAAACAATAGAAGAAGATAGGTGGGATGTTCCAGAAGTTCCAATGGATGAAGACTAGTGGCCAAATATGATCATAGCTCGTTTTCAGGAGTACCTGTGGAGGCTACATTTTTTACTATTATATGCGAT
TTGTTTGCCTTGTAGACAAGCAAGTTCTAAAATATGGGCATATGATATTGATTACCAGTGTTATTTAATTCCGAAAAGGAGGGTCCTTTGACTAGTGATGTATCCTTGTGTGCGCATTGCCATTGTATCATAAAGTTTTGACGGGAAGAGTTGGGGCTGTTCAGTCTGATTTCAAAATAACTAGCATCATATGCAGATTT
AATCGAGTATAATAAAAGAGCAATCAAATTTTATATTTTATATGCGTGGAATGTGTTTTTGGAATTGGAATGGTCTTGGAAATTTAAATCTAGCCTTCTTCTATTATGGAGCATTATTCGATGTTTTCCAAGCCAATTTCACTGTCATATCACTCTCTTAATGTCGGATCAGTAGATTCTGAACATTTTTATTATTCTTT
AATAAATTAATAAGTTTAGATCTTGCT

>TRINITY_DN44969_c0_g3_i5 from sample A6 / Example unspliced isoform:

TAGATAAGCGCCACATTGCTGGCTTGCGTCCGTAGGTTTGCCATTACTTCACTCGTTTGGGTGGTTCGGCGGGCCATCACTTCATGTGgtaagttactgcatgcggcaagttcctgtgcggatgatttcttttgctgtatagttggcggtctaaggtttgtgggcgccgttgtactcttcattgcatgcccatagtgaag
gttctgagaaaattgtaactgcgaattgtctggcgtggacactgtttgtttttgtcatttcacttctctgttttgatcgatccatctacgctgctgcttgaatcagAGGGAGCGTGTTCGAATCGCCGCATTTTCAAGAAGAAGTGTCATTCAATATGGCTCCAGAGCACGCTTCGAATAATGGAGGGATGTCCTCAACC
AAGAAAAAACGGAAGTCTCTAGGGAGTTCAGAAAAACATCAGTTTACATTTGAACAGGAAATGGATGCACAATTTGATAGACCTAGCAAGAAACGCCATGGCAAAGTTCGGAAGCAGCAGGAGAGTGAAGAGTATGTAAGTCAAACAATCAGCAATAAAATATTGAGAGAGGCTCACCGGCAGCAAAAGGAAATTGATAA
TGAGAGGGATGAAGAAGCTGGTTTAGCATCTGCCAAAAAAGCGTTTTCTTTGGTTATAGAGGAGGCCAAATCTGACAGTGATGAGGATGACTTTGATGGGTTTTCTGAAACTCAAAGCCAGTATGAAGGGTATGAAGAGGTACCTGAGGACGATGAAAAGGTATTGGCTGCATTTATGTCTGACAATGTTGGCCCTCAAC
GTACTTTAGCTGATCTTATTATAGAGAGAATCCGAGAAAAAAATGCTGCAGTTTCTTCAGAAGAACGACCCCTTCCTTCACTGGATAATGGAATTATTGATGTTTATAAAGGAGTGGGTAAGCTGCTTAGTCGATACACTGCTGGTAAACTTCCAAAGGCTTTGAAGGTTGTGCCATCATTGTCCCTCTGGGAGGAGGTG
TTATATCTCGCAGAGCCAGAGAAATGGTCACCGAATGCAATGTACCAAGTAACTCGTATTTTTGTATCTAATCTAAATGCCAAGATGGCCCAGCGCTTCTGCATCCTTGTCCTACTTCCACGACTTAGGGAAGACATCAATAAAAATAAGCGACTTCATTTTGCATTGTACCAAGCCTTGAAAAAGTCATTGTACAAGCC
ACCGGCCTTCTTCAAAGGCATTCTCTTGCCTCTCTGTCAGTCACGCACTTGCAATCTGCGGGAAGCTGTCATTATTGGAAGTGTCATTCAGAAAGTCTCGATTCCTTCACTTCATTCTAGTGCTGGTCTAATGAAAATAGCAGAGATGGAGTACTGTGGCACAAACAGTTACTTTATAAAGCTTCTTTTGGACAAAAAGT
ACGCTTTACCATATCGAGTGTTGGATGCTGTTTTTGAACACTTCAAGAGATTTTTTAATGAAACACGTATCATGCCGGTGATATGGCACCAATCCCTTCTGACTTTTGTCCAGAGATACAAGAATGAATTGACGAAGCAGCAGAAGGATGATTTGGAAAACTTGGTTCAACATCAGAAGCATTACTTGGTGACCCCTGAA
ATCCTGAGAGAATTGAGGAACAGTCGAAATCGAGGCGAGAAAGAAAGCGACATTCTCATGTCTACTTTTTCTCCAGTTTCTGTTATTAACAAAACAATAGAAGAAGATAGGTGGGATGTTCCAGAAGTTCCAATGGATGAAGACTAGTGGCCAAATATGATCATAGCTCGTTTTCAGGAGTACCTGTGGAGGCTACATTT
TTTACTATTATATGCGATTTGTTTGCCTTGTAGACAAGCAAGTTCTAAAATATGGGCATATGATATTGATTACCAGTGTTATTTAATTCCGAAAAGGAGGGTCCTTTGACTAGTGATGTATCCTTGTGTGCGCATTGCCATTGTATCATAAAGTTTTGACGGGAAGAGTTGGGGCTGTTCAGTCTGATTTCAAAATAACT
AGCATCATATGCAGATTTAATCGAGTATAATAAAAGAGCAATCAAATTTTATATTTTATATGCGTGGAATGTGTTTTTGGAATTGGAATGGTCTTGGAAATTTAAATCTAGCCTTCTTCTATTATGGAGCATTATTCGATGTTTTCCAAGCCAATTTCACTGTCATATCACTCTCTTAATGTCGGATCAGTAGATTCTGA
ACATTTTTATTATTCTTTAATAAATTAATAAGTTTAGATCTTGCT