sp|Q9SXZ2|FT_ARATH ----> Protein FLOWERING LOCUS T

Intron 1 GTATCTGGTG ... GCAAGATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.02	84	41	121
A2	48.03	66	10	61
A3	57.02	101	27	134
A4	59.38	52	25	76
A5	63.93	22	16	39
A6	57.53	372	97	504
A7	59.26	508	199	739
A8	57.44	569	177	768
A9	57.12	617	184	822
A10	58.28	479	163	669

>TRINITY_DN119047_c2_g6_i7 from sample A2 / Example spliced isoform

AAAAAACCCCTTGAATTACCCCAGATTATTATGGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTAGGTACGTATTTTATTAGACAAACAAGATCCAGGTTATATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGATATTGCT
GATCCACTTCGCCGTGATATAATTGTGGGCAACTCGCGGAGGAATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCGCCGCTGTCTGCTGCTTTTTTTTACATCAAGAAGTAAGAAAATGAGGAGACTGATGATAGTGAATCATCCACATGCAGTATATGAAGTGGCCGAAAGAGATTGGATCAACCAGA
ATCAAGAAAGATGAAGTAGGCCATATACTCTGTGCCATTTGAATGGCTACACCATTTTCTTCATCAACATGTAACCGAAAAGATTCAAGAAAGATGAAGGAACAGAAAAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACATCTTTTACTGTTCTGGATATTGACATTGAGTACGCTACAGTACCATTTATCT
CTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTGCGAAATACAAGATCCATTTATCTCTTATATGAAATACAATACAACTTTGAAG

>TRINITY_DN119047_c2_g6_i5 from sample A2 / Example unspliced isoform:

AAAAAACCCCTTGAATTACCCCAGATTATTATGGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTAGGTACGTATTTTATTAGACAAACAAGATCCAGgtatctggtgtgtttttttctttgaccctgaatcgatgtatggagtacacgccgtattgtgaaccgtgatgaatgtttgtttcttt
gcgctgcaagattagGTTATATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGATATTGCTGATCCACTTCGCCGTGATATAATTGTGGGCAACTCGCGGAGGAATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCGCCGCTGTCTGCT
GCTTTTTTTTACATCAAGAAGTAAGAAAATGAGGAGACTGATGATAGTGAATCATCCACATGCAGTATATGAAGTGGCCGAAAGAGATTGGATCAACCAGAATCAAGAAAGATGAAGTAGGCCATATACTCTGTGCCATTTGAATGGCTACACCATTTTCTTCATCAACATGTAACCGAAAAGATTCAAGAAAGATGAAG
GAACAGAAAAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACATCTTTTACTGTTCTGGATATTGACATTGAGTACGCTACAGTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTGCGAAATACAAGATCCATTTATCTCTTATATGAAATACAATACAACTTTGAAG

Intron 2 GCGAGTATTT ... GCAAGATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.68	5	14	14
A2	62.5	3	5	5
A3	60	15	17	28
A4	80.65	3	11	14
A5	57.14	6	5	11
A6	55.46	51	39	88
A7	67.72	56	96	139
A8	56.52	85	72	149
A9	51.86	97	97	112
A10	61.17	60	65	124

>TRINITY_DN48661_c2_g5_i5 from sample A7 / Example spliced isoform

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGGTTATATAT
CATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACATGCTGGTTTCCACGTTGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGAAAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAACCG
GCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAGTAGGCTATATACTCTGTGCCATTTGAATGGCTATACACCATTTTCTTCATGTCATCAACATCTTTTACGGTTCTTGATATTGACATTGAGTACGCTACACTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTG
CGATAACGTTTTGTAGCTGTTATTTTGAAGGTGTTCTGTAATCACATTTTATTTGGTTTCACAGTATTATTTATGAACATGACTTCTTTCCAGGAAATAAATTATAAATAGAGAACTGAATCAAGTTAGGACCCATTATACATTCTTACTAATGGAGATATCAAAGATCAACACGGC

>TRINITY_DN48661_c2_g5_i7 from sample A7 / Example unspliced isoform:

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGgcgagtatt
tgcactgttaggtacgtattttattagacaaacaagatccaagtatctggtgtgtgtttttctttgaccctgaattgatgtatggagtacacgccctattgtgaaccgtgatgaatgtttatttctttgggttgcaagattagGTTATATATCATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACA
TGCTGGTTTCCACGTTGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGAAAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAACCGGCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAGTAGGCTA
TATACTCTGTGCCATTTGAATGGCTATACACCATTTTCTTCATGTCATCAACATCTTTTACGGTTCTTGATATTGACATTGAGAGATCCTATGGTAAAAATCTGTACGGATAATGGTGGTTTTCAAACAAAGAATGGTCATCAATTATGTATTGAGTACGTTAGTTTGGAAGAAACCGTTCGTTTCAGCTCATTTGGCAG
ATGTGATTTGATGCATACTTTGTTAAGAAATGGTTTTATTTTCAATGTTACTTTGCATGTGATTGCTTATGTCTGCATCCTGCCGATCCTAAGGCAAGGATGCAGACATGGGTGAGTTGGGTGGTCATGTGGTGGACAGAAAGGCAATGCAAGCAACGAACTTTCTTCTCTGTTGTGCTTAACCGGATTGGTCTGTTCTT
CCACCTTCTCCATGACTATTTATTCCTCGGGAGCTTAGAATGTACAAATAAATTTTGGAGGAAATGTAAAGTATGTATTATATATTGATTGATCATTCCAAAGGCTCGGATATGTGCTGTTCTTTATTGTCATTATCATTACCTAAACATTATATATGTAAATATTCA

Intron 3 GTTATGTATA ... CGTTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.56	14	9	26
A2	52	6	5	8
A3	48.72	10	7	12
A4	27.27	12	5	4
A5	33.33	5	2	3
A6	53.85	36	36	48
A7	42.37	102	68	82
A8	51.52	64	61	75
A9	42.15	94	49	88
A10	39.64	67	48	40

>TRINITY_DN48661_c2_g5_i2 from sample A7 / Example spliced isoform

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGGCGAGTATT

TGCACTGTTAGGTACGTATTTTATTAGACAAACAAGATCCAAGTATCTGGTGTGTGTTTTTCTTTGACCCTGAATTGATGTATGGAGTACACGCCCTATTGTGAACCGTGATGAATGTTTATTTCTTTGGGTTGCAAGATTAGGTTATATATCATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACA
TGCTGGTTTCCACGTTGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGAAAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAACCGGCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAGTAGGCTA
TATACTCTGTGCCATTTGAATGGCTATACACCATTTTCTTCATGTCATCAACATCTTTTACGGTTCTTGATATTGACATTGAGTACGCTACACTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTGCGATAACGTTTTGTAGCTGTTATTTTGAAGGTGTTCTGTAATCACATT
TTATTTGGTTTCACAGTATTATTTATGAACATGACTTCTTTCCAGGAAATAAATTATAAATAGAGAACTGAATCAAGTTAGGACCCATTATACATTCTTACTAATGGAGATATCAAAGATCAACACGGC

>TRINITY_DN48661_c2_g5_i1 from sample A7 / Example unspliced isoform:

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGGCGAGTATT

TGCACTgttatgtataattctcctacatagatcaaatttctaaattatagcacatgaatccattaaaaattttcataataacagttgaacggtacgttgtacagGTTAGGTACGTATTTTATTAGACAAACAAGATCCAAGTATCTGGTGTGTGTTTTTCTTTGACCCTGAATTGATGTATGGAGTACACGCCCTATTGT
GAACCGTGATGAATGTTTATTTCTTTGGGTTGCAAGATTAGGTTATATATCATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACATGCTGGTTTCCACGTTGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGA
AAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAACCGGCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAGTAGGCTATATACTCTGTGCCATTTGAATGGCTATACACCATTTTCTTCATGTCATCAACATCTTTTACGGTTCTTGATATTGACATTGAGTACGCTACACTACCATTTA
TCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTGCGATAACGTTTTGTAGCTGTTATTTTGAAGGTGTTCTGTAATCACATTTTATTTGGTTTCACAGTATTATTTATGAACATGACTTCTTTCCAGGAAATAAATTATAAATAGAGAACTGAATCAAGTTAGGACCCATTATACATTCTTACT
AATGGAGATATCAAAGATCAACACGGC

Intron 4 GTACGTATTT ... GCAATATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.53	26	15	19
A2	33.33	19	3	16
A3	35.56	29	4	28
A4	32.5	27	2	24
A5	48.15	7	4	9
A6	39.36	104	33	102
A7	40.63	179	75	170
A8	38.58	207	73	187
A9	39.21	200	65	193
A10	40.19	160	59	156

>TRINITY_DN48732_c3_g1_i2 from sample A10 / Example spliced isoform

CCAACCTGTTGAACTACAGCCTGCTGAAGTGCCAATCCAGCCTGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGCTGAAGTGCCAATCCAGCCTGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGTTGAAGTGCAAACTCAGCCTGTTGAAGCTGTTGAAGTGCAAACCC
AACCTGTTGAACTACAGCCTGTTGAAGTGCAAACGTTGAAGTGCAAACCCAGCATGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGCTGAAGTGCCAATTCAGCCTGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGTTGAAGTGCAAACATGAAGTGCAAACCCAACTT
GGTTATGATCTTGATCCCGAGTTTACTGAATTCTTGAATAACCCCTTGAAAATCCGCAGATTCTTATTGTTTGCCGAAAGAATGGGCATCATATTTAAAGAGATAAGGGGGAAGCTGCTGGAATCAAAAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGATGAAGCTCTCTGTATTCTATGGCGATAAAGAAAT
CAGCTTAGGTGATGAACTATTGCCTGTTCAAGTGCAAAGCTATGACAGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTAGGTTTTTCTGGATCCTCGACGGAGCTTTTTGTCTATGAACCTCCGAGGCCGGGTGTGCACATGCTGGTTTTCACGTTGTTCGATTTTGGCGATCGACTTCATAGCGATATAAAT
GTGGGCAACTCGCGGAGGAATTTCAGCACTAGAGATTTTGCTCGTGATAATGCACTTGGACCGCCGCTGTCTGCTACTTTTTTTTACATCAAGTATGAAAATGAGGAGAGTGATGATACTGATTGTAGGAAAGAGACTGGATCAACCAGCGGCACTAAAAAAGAATGCAGCATCAAGAAAGATGAAGTAGCCGAAAAGAT
TCAAGAAAGATGAAGTAAAAGAAGAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACATCTTTTACTGTTCTTGAGTGAGTGCTGATGTTTGATATTGAGATAGAGCTCAATTACATTGAGTACGCTACAGTACAATTAATTTTTTATATGAAATACAAGATCTTTTCATTTATCTTTTATATGAAATACAAGA
TCTTTTCATTTATCTTTTATATGAAATACAAGATCTTTTCATTTATCTTTTATATGAAATACAAGAT

>TRINITY_DN48732_c3_g1_i22 from sample A10 / Example unspliced isoform:

CCAACCTGTTGAACTACAGCCTGCTGAAGTGCCAATCCAGCCTGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGCTGAAGTGCCAATCCAGCCTGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGTTGAAGTGCAAACTCAGCCTGTTGAAGCTGTTGAAGTGCAAACCC
AACCTGTTGAACTACAGCCTGTTGAAGTGCAAACGTTGAAGTGCAAACCCAGCATGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGCTGAAGTGCCAATTCAGCCTGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGTTGAAGTGCAAACATGAAGTGCAAACCCAACTT
GGTTATGATCTTGATCCCGAGTTTACTGAATTCTTGAATAACCCCTTGAAAATCCGCAGATTCTTATTGTTTGCCGAAAGAATGGGCATCATATTTAAAGAGATAAGGGGGAAGCTGCTGGAATCAAAAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGATGAAGCTCTCTGTATTCTATGGCGATAAAGAAAT
CAGCTTAGGTGATGAACTATTGCCTGTTCAAGTGCAAAGCTATGACAGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTAGgtacgtattttattaaacaccaaagaagatccaggtatctggtgtgtttttttctttggccctgaatcgatggatggagtacacgccgtaatgtcaaccgtgatgaatgtttc
tttctttgcgctgcaatattagGTTTTTCTGGATCCTCGACGGAGCTTTTTGTCTATGAACCTCCGAGGCCGGGTGTGCACATGCTGGTTTTCACGTTGTTCGATTTTGGCGATCGACTTCATAGCGATATAAATGTGGGCAACTCGCGGAGGAATTTCAGCACTAGAGATTTTGCTCGTGATAATGCACTTGGACCGCC
GCTGTCTGCTACTTTTTTTTACATCAAGTATGAAAATGAGGAGAGTGATGATACTGATTGTAGGAAAGAGACTGGATCAACCAGCGGCACTAAAAAAGAATGCAGCATCAAGAAAGATGAAGTAGCCGAAAAGATTCAAGAAAGATGAAGTAAAAGAAGAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACAT
CTTTTACTGTTCTTGAGTGAGTGCTGATGTTTGATATTGAGATAGAGCTCAATTACATTGAGTACGCTACAGTACAATTAATTTTTTATATGAAATACAAGATCTTTTCATTTATCTTTTATATGAAATACAAGATCTTTTCATTTATCTTTTATATGAAATACAAGATCTTTTCATTTATCTTTTATATGAAATACAAG
AT

Intron 5 GTATAATTCT ... GCAAGATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.65	62	9	14
A2	12.5	35	5	5
A3	21.69	65	8	28
A4	12.75	65	5	14
A5	20	26	2	11
A6	18.37	280	35	91
A7	21.45	379	67	140
A8	21.55	384	62	149
A9	15.05	446	46	112
A10	21.82	310	47	126

>TRINITY_DN48661_c2_g5_i8 from sample A7 / Example spliced isoform

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGGCGAGTATT
TGCACTGTTATGTTATATATCATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACATGCTGGTTTCCACGTTGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGAAAATGAGGAGACTGATGATAGTGAATCATC
CACATCCAGTATATGAACCGGCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAG

>TRINITY_DN48661_c2_g5_i4 from sample A7 / Example unspliced isoform:

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGGCGAGTATT
TGCACTGTTATgtataattctcctacatagatcaaatttctaaattatagcacatgaatccattaaaaattttcataataacagttgaacggtacgttgtacaggttaggtacgtattttattagacaaacaagatccaagtatctggtgtgtgtttttctttgaccctgaattgatgtatggagtacacgccctattgt
gaaccgtgatgaatgtttatttctttgggttgcaagattagGTTATATATCATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACATGCTGGTTTCCACGTTGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGA
AAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAACCGGCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAGAGATCCTATGGTAAAAATCTGTACGGATAATGGTGGTTTTCAAACAAAGAATGGTCATCAATTATGTATTGAGTACGTTAGTTTGGAAGAAACCGTTCGTTTCAGCTCA
TTTGGCAGATGTGATTTGATGCATACTTTGTTAAGAAATGGTTTTATTTTCAATGTTACTTTGCATGTGATTGCTTATGTCTGCATCCTGCCGATCCTAAGGCAAGGATGCAGACATGGGTGAGTTGGGTGGTCATGTGGTGGACAGAAAGGCAATGCAAGCAACGAACTTTCTTCTCTGTTGTGCTTAACCGGATTGGT
CTGTTCTTCCACCTTCTCCATGACTATTTATTCCTCGGGAGCTTAGAATGTACAAATAAATTTTGGAGGAAATGTAAAGTATGTATTATATATTGATTGATCATTCCAAAGGCTCGGATATGTGCTGTTCTTTATTGTCATTATCATTACCTAAACATTATATATGTAAATATTCA

Intron 6 GTAAGTTTAA ... ACATTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.19	298.5	119	21.5
A2	24.66	221.5	71.8	4.5
A3	21.88	243.5	67	8.5
A4	20.84	229.3	59	3.3
A5	26.41	101.5	35.3	2.8
A6	22.02	1141.8	315	28.3
A7	19.72	1596	383	29.8
A8	23.75	1535.5	468.3	54.8
A9	18.99	1795.3	409.5	21.3
A10	21.01	1356.8	351.8	12.3

>TRINITY_DN109696_c1_g1_i4 from sample A5 / Example spliced isoform

TGTTGAACCAGTTGAAGTGCAAACCCAACTTGGTTATGATCTTGATCCCGAGCTTGCTGAATTCTTGAATAACCCCTTGAATGTCCGCAGATTATTATTGTTTGCCGAAAGAATGGGCAACATATTGGACGAGATAAAGGGGAAGCTGCTGGAATCGAGAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGCTGA
AGTTCTCTGTAAGCTATGGCGATAAGAAAATCAGCTTAGGTGATGAACTATTGCCTGTTCAAGTGCAAAGCGAACCTATCCTTTTTGGAGTTTCTGGTTATGATCTTGATCCCGAGTTAATTGAAATCTTGAATAACCCCTTGAATTACCCCAGATTATTATGGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTAT
TTGCACTGGTTATATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGATATTGCTGATCCACTTCGCCGTGATATAATTGTGGGCAACTCGCGGAGGAATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCGCCGCTGTCTGCTGCTTTTT
TTTACATCAAGAAGTAAGAAAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAAGTGGCCGAAAGAGATTGGATCAACCAGAATCAAGAAAGATGAAGTAGGCCATATACTCTGTGCCATTTGAATGGCTACACCATTTTCTTCATCAACATGTAACCGAAAAGATTCAAGAAAGATGAAGGAACAGA
AAAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACATCTTTTACTGTTCTGGATATTGACATTGAGTACGCTACAGTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTGCGAAATACAAGATCCATTTATCT

>TRINITY_DN109696_c1_g1_i9 from sample A5 / Example unspliced isoform:

TGTTGAACCAGTTGAAGTGCAAACCCAACTTGGTTATGATCTTGATCCCGAGCTTGCTGAATTCTTGAATAACCCCTTGAATGTCCGCAGATTATTATTGAATGGGCAACATATTGGACGAGATAAAGGGGAAGCTGCTGGAATCGAGAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGCTGAAGTTCTCTGTA
AGCTATGGCGATAAGAAAATCAGCTTAGGTGATGAACTATTGCCTGTTCAAGTGCAAAGCGAACCTATCCTTTTTGGAGTTTCTGGTTATGATCTTGATCCCGAGTTAATTGAAATCTTGAATAACCCCTTGAATTACCCCAGATTATTATGGgtaagtttaattcctttttcgtcaatatatgatattttgtcaaattg
aaattcatgttaaccacattttcagGTTATGACGGATCCTGCTCCCC

Intron 7 GCGAGTATTT ... CGTTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	81.82	4	10	26
A2	52	6	5	8
A3	47.37	15	15	12
A4	47.37	5	5	4
A5	37.5	5	3	3
A6	58.67	31	40	48
A7	50.52	71	63	82
A8	57.31	54	70	75
A9	51.6	68	57	88
A10	56.41	34	48	40

>TRINITY_DN48661_c2_g5_i6 from sample A7 / Example spliced isoform

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGGTTAGGTAC
GTATTTTATTAGACAAACAAGATCCAAGTATCTGGTGTGTGTTTTTCTTTGACCCTGAATTGATGTATGGAGTACACGCCCTATTGTGAACCGTGATGAATGTTTATTTCTTTGGGTTGCAAGATTAGGTTATATATCATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACATGCTGGTTTCCACGT
TGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGAAAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAACCGGCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAGTAGGCTATATACTCTGTGCCAT
TTGAATGGCTATACACCATTTTCTTCATGTCATCAACATCTTTTACGGTTCTTGATATTGACATTGAGTACGCTACACTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTGCGATAACGTTTTGTAGCTGTTATTTTGAAGGTGTTCTGTAATCACATTTTATTTGGTTTCACA
GTATTATTTATGAACATGACTTCTTTCCAGGAAATAAATTATAAATAGAGAACTGAATCAAGTTAGGACCCATTATACATTCTTACTAATGGAGATATCAAAGATCAACACGGC

>TRINITY_DN48661_c2_g5_i4 from sample A7 / Example unspliced isoform:

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGgcgagtatt
tgcactgttatgtataattctcctacatagatcaaatttctaaattatagcacatgaatccattaaaaattttcataataacagttgaacggtacgttgtacagGTTAGGTACGTATTTTATTAGACAAACAAGATCCAAGTATCTGGTGTGTGTTTTTCTTTGACCCTGAATTGATGTATGGAGTACACGCCCTATTGT
GAACCGTGATGAATGTTTATTTCTTTGGGTTGCAAGATTAGGTTATATATCATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACATGCTGGTTTCCACGTTGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGA
AAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAACCGGCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAGAGATCCTATGGTAAAAATCTGTACGGATAATGGTGGTTTTCAAACAAAGAATGGTCATCAATTATGTATTGAGTACGTTAGTTTGGAAGAAACCGTTCGTTTCAGCTCA
TTTGGCAGATGTGATTTGATGCATACTTTGTTAAGAAATGGTTTTATTTTCAATGTTACTTTGCATGTGATTGCTTATGTCTGCATCCTGCCGATCCTAAGGCAAGGATGCAGACATGGGTGAGTTGGGTGGTCATGTGGTGGACAGAAAGGCAATGCAAGCAACGAACTTTCTTCTCTGTTGTGCTTAACCGGATTGGT
CTGTTCTTCCACCTTCTCCATGACTATTTATTCCTCGGGAGCTTAGAATGTACAAATAAATTTTGGAGGAAATGTAAAGTATGTATTATATATTGATTGATCATTCCAAAGGCTCGGATATGTGCTGTTCTTTATTGTCATTATCATTACCTAAACATTATATATGTAAATATTCA

Intron 8 GTACGTATTT ... CAAGATCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.75	230	13	84
A2	36.07	117	27	66
A3	32.48	210	29	101
A4	26.4	145	4	52
A5	24.18	69	5	22
A6	30.32	855	77	372
A7	29.06	1240	96	508
A8	30.14	1319	116	569
A9	29.23	1494	102	617
A10	30.51	1091	80	479

>TRINITY_DN119047_c2_g6_i3 from sample A2 / Example spliced isoform

AAAAAACCCCTTGAATTACCCCAGATTATTATGGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTAGGTTATATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGATATTGCTGATCCACTTCGCCGTGATATAATTGTGGGCA
ACTCGCGGAGGAATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCGCCGCTGTCTGCTGCTTTTTTTTACATCAAGAAGTAAGAAAATGAGGAGACTGATGATAGTGAATCATCCACATGCAGTATATGAAGTGGCCGAAAGAGATTGGATCAACCAGAATCAAGAAAGATGAAGTAGGCCATATACTCT
GTGCCATTTGAATGGCTACACCATTTTCTTCATCAACATGTAACCGAAAAGATTCAAGAAAGATGAAGGAACAGAAAAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACATCTTTTACTGTTCTGGATATTGACATTGAGTACGCTACAGTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCA
TTCTGGGTTATTTCTGCGAAATACAAGATCCATTTATCTCTTATATGAAATACAATACAACTTTGAAG

>TRINITY_DN119047_c2_g6_i7 from sample A2 / Example unspliced isoform:

AAAAAACCCCTTGAATTACCCCAGATTATTATGGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTAGgtacgtattttattagacaaacaagatccagGTTATATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGATATTGCT
GATCCACTTCGCCGTGATATAATTGTGGGCAACTCGCGGAGGAATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCGCCGCTGTCTGCTGCTTTTTTTTACATCAAGAAGTAAGAAAATGAGGAGACTGATGATAGTGAATCATCCACATGCAGTATATGAAGTGGCCGAAAGAGATTGGATCAACCAGA
ATCAAGAAAGATGAAGTAGGCCATATACTCTGTGCCATTTGAATGGCTACACCATTTTCTTCATCAACATGTAACCGAAAAGATTCAAGAAAGATGAAGGAACAGAAAAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACATCTTTTACTGTTCTGGATATTGACATTGAGTACGCTACAGTACCATTTATCT
CTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTGCGAAATACAAGATCCATTTATCTCTTATATGAAATACAATACAACTTTGAAG

Intron 9 GTAAGTTTAA ... CCATAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.49	304.7	122	20
A2	24.9	223.3	73	5
A3	23.08	249	73	9
A4	21.47	230	61	3
A5	27.86	102.7	38	7
A6	22.46	1156.3	325	24
A7	20.44	1621.3	404	40
A8	24.71	1577	504	64
A9	19.52	1825	427	25
A10	21.33	1377	361	13

>TRINITY_DN109696_c1_g1_i4 from sample A5 / Example spliced isoform

TGTTGAACCAGTTGAAGTGCAAACCCAACTTGGTTATGATCTTGATCCCGAGCTTGCTGAATTCTTGAATAACCCCTTGAATGTCCGCAGATTATTATTGTTTGCCGAAAGAATGGGCAACATATTGGACGAGATAAAGGGGAAGCTGCTGGAATCGAGAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGCTGA
AGTTCTCTGTAAGCTATGGCGATAAGAAAATCAGCTTAGGTGATGAACTATTGCCTGTTCAAGTGCAAAGCGAACCTATCCTTTTTGGAGTTTCTGGTTATGATCTTGATCCCGAGTTAATTGAAATCTTGAATAACCCCTTGAATTACCCCAGATTATTATGGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTAT
TTGCACTGGTTATATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGATATTGCTGATCCACTTCGCCGTGATATAATTGTGGGCAACTCGCGGAGGAATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCGCCGCTGTCTGCTGCTTTTT
TTTACATCAAGAAGTAAGAAAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAAGTGGCCGAAAGAGATTGGATCAACCAGAATCAAGAAAGATGAAGTAGGCCATATACTCTGTGCCATTTGAATGGCTACACCATTTTCTTCATCAACATGTAACCGAAAAGATTCAAGAAAGATGAAGGAACAGA
AAAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACATCTTTTACTGTTCTGGATATTGACATTGAGTACGCTACAGTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTGCGAAATACAAGATCCATTTATCT

>TRINITY_DN109696_c1_g1_i10 from sample A5 / Example unspliced isoform:

TGTTGAACCAGTTGAAGTGCAAACCCAACTTGGTTATGATCTTGATCCCGAGCTTGCTGAATTCTTGAATAACCCCTTGAATGTCCGCAGATTATTATTGTTTGCCGAAAGAATGGGCAACATATTGGACGAGATAAAGGGGAAGCTGCTGGAATCGAGAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGCTGA
AGTTCTCTGTAAGCTATGGCGATAAGAAAATCAGCTTAGGTGATGAACTATTGCCTGTTCAAGTGCAAAGCGAACCTATCCTTTTTGGAGTTTCTGGTTATGATCTTGATCCCGAGTTAATTGAAATCTTGAATAACCCCTTGAATTACCCCAGATTATTATGGgtaagtttaattcctttttcgtcaatatatgatatt
ttgtcaaattgaaattcatgttaacgatattttcataaacccataaacagGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTA

Intron 10 GCGAGTATTT ... GCAAGATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70.59	5	10	14
A2	62.5	3	5	5
A3	58.9	15	15	28
A4	76	3	5	14
A5	53.85	6	3	11
A6	56.22	51	40	91
A7	64.44	56	63	140
A8	56.3	85	70	149
A9	46.56	97	57	112
A10	59.18	60	48	126

>TRINITY_DN48661_c2_g5_i5 from sample A7 / Example spliced isoform

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGGTTATATAT
CATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACATGCTGGTTTCCACGTTGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGAAAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAACCG
GCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAGTAGGCTATATACTCTGTGCCATTTGAATGGCTATACACCATTTTCTTCATGTCATCAACATCTTTTACGGTTCTTGATATTGACATTGAGTACGCTACACTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTG
CGATAACGTTTTGTAGCTGTTATTTTGAAGGTGTTCTGTAATCACATTTTATTTGGTTTCACAGTATTATTTATGAACATGACTTCTTTCCAGGAAATAAATTATAAATAGAGAACTGAATCAAGTTAGGACCCATTATACATTCTTACTAATGGAGATATCAAAGATCAACACGGC

>TRINITY_DN48661_c2_g5_i3 from sample A7 / Example unspliced isoform:

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGgcgagtatt
tgcactgttatgtataattctcctacatagatcaaatttctaaattatagcacatgaatccattaaaaattttcataataacagttgaacggtacgttgtacaggttaggtacgtattttattagacaaacaagatccaagtatctggtgtgtgtttttctttgaccctgaattgatgtatggagtacacgccctattgt
gaaccgtgatgaatgtttatttctttgggttgcaagattagGTTATATATCATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACATGCTGGTTTCCACGTTGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGA
AAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAACCGGCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAGTAGGCTATATACTCTGTGCCATTTGAATGGCTATACACCATTTTCTTCATGTCATCAACATCTTTTACGGTTCTTGATATTGACATTGAGAGATCCTATGGTAAAAATC
TGTACGGATAATGGTGGTTTTCAAACAAAGAATGGTCATCAATTATGTATTGAGTACGTTAGTTTGGAAGAAACCGTTCGTTTCAGCTCATTTGGCAGATGTGATTTGATGCATACTTTGTTAAGAAATGGTTTTATTTTCAATGTTACTTTGCATGTGATTGCTTATGTCTGCATCCTGCCGATCCTAAGGCAAGGATG
CAGACATGGGTGAGTTGGGTGGTCATGTGGTGGACAGAAAGGCAATGCAAGCAACGAACTTTCTTCTCTGTTGTGCTTAACCGGATTGGTCTGTTCTTCCACCTTCTCCATGACTATTTATTCCTCGGGAGCTTAGAATGTACAAATAAATTTTGGAGGAAATGTAAAGTATGTATTATATATTGATTGATCATTCCAAA
GGCTCGGATATGTGCTGTTCTTTATTGTCATTATCATTACCTAAACATTATATATGTAAATATTCA

Intron 11 GTGATGAACT ... ACATTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.73	157	548	32
A2	76.8	142	470	9
A3	78.06	156	555	16
A4	75.7	148	461	7
A5	80.71	49	205	5
A6	82.71	469	2244	47
A7	83.48	626	3163	59
A8	81.62	666	2958	81
A9	83.33	634	3170	31
A10	82.38	574	2683	34

>TRINITY_DN48732_c3_g1_i26 from sample A10 / Example spliced isoform

GGAGAAGCTGGTGGAATCGGGAGTTATCGGAACTGTTGTCGACGATGAACTATTGCCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGTTGAAGTGCAAACCCAACCTGTTGAAGTGCAAACCCAGCATGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGCTGAAGTGCCAATTCAGC
CTGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGTTGAAGTGCAAACCTGAAGTGCAAACCCAGCCTGTTGAACCAGATGAAGTGCAAACCCAACTTGGTTATGATCTTGATCCCGAGCTTGCTGAATTCTTGCATAACCCCTTGAATGTCCGCAGATTATTATTGAATGGGCAACATATTGGACG
AGATAAAGGGGAAGCTGCTGGAATCGAGAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGCTGAAGTTCTCTGTAAGCTATGGCGATAAGAAAATCAGCTTAGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTAGGT

>TRINITY_DN48732_c3_g1_i4 from sample A10 / Example unspliced isoform:

GGAGAAGCTGGTGGAATCGGGAGTTATCGGAACTGTTGTCGACGATGAACTATTGCCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGTTGAAGTGCAAACCCAACCTGTTGAAGTGCAAACCCAGCATGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGCTGAAGTGCCAATTCAGC
CTGTTGAAGCTGTTGAAGTGCAAACCCAACCTGTTGAACTACAGCCTGTTGAAGTGCAAACCTGAAGTGCAAACCCAGCCTGTTGAACCAGATGAAGTGCAAACCCAACTTGGTTATGATCTTGATCCCGAGCTTGCTGAATTCTTGAATAACCCCTTGAAAATCCGCAGATTCTTATTGTTTGCCGAAAGAATGGGCAA
CATATTGGACGAGATAAAGGGGAAGCTGCTGGAATCGAGAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGCTGAAGTTCTCTGTAAGCTATGGCGATAAGAAAATCAGCTTAGgtgatgaactattgcctgttcaagtgcaaagcgaacctatcctttttggagtttctggttatgatcttgatcccgagttaa
ttgaaatcttgaattaccccttgaattaccccagattattatgggtaagtttaattcctttttcgtcaatatatgatattttgtcaaattgaaattcatgttaaccacattttcagGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTATGCTATAATTCTCCTACATAGATCAAATTTCTAAATT
ATAGCACATGAATCCATTAAAAATTTTCATAATAACAGTTGAACGGTACGTTGTACAGGTTAGGTACGTATTTTATTAGACAAACAAGATCCAGGTATCTGGTGTGTTTTTTTCTTTGACCCTGAATCGATGTATGGAGTACACGCCGTATTGTGAACCGTGATGAATGTTTGTTTCTTTGCGCTGCAAGATTAGGTTAT
ATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGATATTGCTGATCCACTTCGCCGTGATATAATTGTGGGCAACTCG

Intron 12 GTACGTATTT ... GCAAGATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	34.47	230	24	121
A2	34.27	117	11	61
A3	38.95	210	14	134
A4	34.39	145	17	76
A5	36.11	69	12	39
A6	37.09	855	45	504
A7	37.34	1240	116	739
A8	36.8	1319	105	768
A9	35.49	1494	124	822
A10	38.01	1091	96	669

>TRINITY_DN119047_c2_g6_i3 from sample A2 / Example spliced isoform

AAAAAACCCCTTGAATTACCCCAGATTATTATGGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTAGGTTATATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGATATTGCTGATCCACTTCGCCGTGATATAATTGTGGGCA
ACTCGCGGAGGAATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCGCCGCTGTCTGCTGCTTTTTTTTACATCAAGAAGTAAGAAAATGAGGAGACTGATGATAGTGAATCATCCACATGCAGTATATGAAGTGGCCGAAAGAGATTGGATCAACCAGAATCAAGAAAGATGAAGTAGGCCATATACTCT
GTGCCATTTGAATGGCTACACCATTTTCTTCATCAACATGTAACCGAAAAGATTCAAGAAAGATGAAGGAACAGAAAAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACATCTTTTACTGTTCTGGATATTGACATTGAGTACGCTACAGTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCA
TTCTGGGTTATTTCTGCGAAATACAAGATCCATTTATCTCTTATATGAAATACAATACAACTTTGAAG

>TRINITY_DN119047_c2_g6_i5 from sample A2 / Example unspliced isoform:

AAAAAACCCCTTGAATTACCCCAGATTATTATGGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTAGgtacgtattttattagacaaacaagatccaggtatctggtgtgtttttttctttgaccctgaatcgatgtatggagtacacgccgtattgtgaaccgtgatgaatgtttgtttcttt
gcgctgcaagattagGTTATATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGATATTGCTGATCCACTTCGCCGTGATATAATTGTGGGCAACTCGCGGAGGAATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCGCCGCTGTCTGCT
GCTTTTTTTTACATCAAGAAGTAAGAAAATGAGGAGACTGATGATAGTGAATCATCCACATGCAGTATATGAAGTGGCCGAAAGAGATTGGATCAACCAGAATCAAGAAAGATGAAGTAGGCCATATACTCTGTGCCATTTGAATGGCTACACCATTTTCTTCATCAACATGTAACCGAAAAGATTCAAGAAAGATGAAG
GAACAGAAAAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACATCTTTTACTGTTCTGGATATTGACATTGAGTACGCTACAGTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTGCGAAATACAAGATCCATTTATCTCTTATATGAAATACAATACAACTTTGAAG

Intron 13 GTTAGGTACG ... GCAAGATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.29	5	4	14
A2	64.71	3	6	5
A3	58.9	15	15	28
A4	76	3	5	14
A5	57.14	6	5	11
A6	54.05	51	31	89
A7	65.54	56	71	142
A8	54.55	85	54	150
A9	48.13	97	68	112
A10	56.83	60	34	124

>TRINITY_DN48661_c2_g5_i5 from sample A7 / Example spliced isoform

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGGTTATATAT
CATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACATGCTGGTTTCCACGTTGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGAAAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAACCG
GCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAGTAGGCTATATACTCTGTGCCATTTGAATGGCTATACACCATTTTCTTCATGTCATCAACATCTTTTACGGTTCTTGATATTGACATTGAGTACGCTACACTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTG
CGATAACGTTTTGTAGCTGTTATTTTGAAGGTGTTCTGTAATCACATTTTATTTGGTTTCACAGTATTATTTATGAACATGACTTCTTTCCAGGAAATAAATTATAAATAGAGAACTGAATCAAGTTAGGACCCATTATACATTCTTACTAATGGAGATATCAAAGATCAACACGGC

>TRINITY_DN48661_c2_g5_i6 from sample A7 / Example unspliced isoform:

CTTAAAACGGTTGCCATGCGGGGAAGGAAAGGGAAATCGTTTCAATATATGGAAGCCAAAGGGCAATGGGAGCAAGCGAAAAATGGAATGAGCGGATCATTCAGGTGGAGCAAAATAGGAGGATACATCGAAGGATCGATTACAATCTCCGGTTATCCTAAGGAGCAGGAAACTTTTGCAAGGATTTCTGGTTACTGTGA
ACAAAATGGAATCATCTCTAAACTGTAATTCACCACAGAGACGGATGAATTTGAAGTTGCGTATTCTGATGAAAGCAATGTGCAGGGCGTTAACAGATTTGTTGATGCCGAGTTTCTGGTTATGATCTTGAATAACCTCTTGAATTACCCCAGATTATTATTGGTTATGACGGATCGTGCTCCTCCTCACGgttaggtac
gtattttattagacaaacaagatccaagtatctggtgtgtgtttttctttgaccctgaattgatgtatggagtacacgccctattgtgaaccgtgatgaatgtttatttctttgggttgcaagattagGTTATATATCATTGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCTCGGTGCGCACATGCTGGTTTCCACGT
TGTTCGATATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCTCCACTGTCTGCTGCGTTTTTTTACATCAAGTATGAAAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAACCGGCCGAAAGAGATTGGATCAACCAAAATCAAGAAAGATGAAGTAGGCTATATACTCTGTGCCAT
TTGAATGGCTATACACCATTTTCTTCATGTCATCAACATCTTTTACGGTTCTTGATATTGACATTGAGTACGCTACACTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTGCGATAACGTTTTGTAGCTGTTATTTTGAAGGTGTTCTGTAATCACATTTTATTTGGTTTCACA
GTATTATTTATGAACATGACTTCTTTCCAGGAAATAAATTATAAATAGAGAACTGAATCAAGTTAGGACCCATTATACATTCTTACTAATGGAGATATCAAAGATCAACACGGC

Intron 14 GTGATGAACT ... CCATAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.13	224	552	20
A2	72.46	179	471	5
A3	71.79	220	560	9
A4	71.52	186	467	3
A5	75.27	68	207	7
A6	75.28	746	2272	24
A7	75.53	1034	3191	40
A8	74.08	1048	2995	64
A9	74.98	1070	3206	25
A10	75.08	907	2732	13

>TRINITY_DN109696_c1_g1_i2 from sample A5 / Example spliced isoform

TGTTGAACCAGTTGAAGTGCAAACCCAACTTGGTTATGATCTTGATCCCGAGCTTGCTGAATTCTTGAATAACCCCTTGAATGTCCGCAGATTATTATTGTTTGCCGAAAGAATGGGCAACATATTGGACGAGATAAAGGGGAAGCTGCTGGAATCGAGAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGCTGA
AGTTCTCTGTAAGCTATGGCGATAAGAAAATCAGCTTAGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTATATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGATATTGCTGATCCACTTCGCCGTGATATAATTGTGGGCA
ACTCGCGGAGGAATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCGCCGCTGTCTGCTGCTTTTTTTTACATCAAGAAGTAAGAAAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAAGTGGCCGAAAGAGATTGGATCAACCAGAATCAAGAAAGATGAAGTAGGCCATATACTCT
GTGCCATTTGAATGGCTACACCATTTTCTTCATCAACATGTAACCGAAAAGATTCAAGAAAGATGAAGGAACAGAAAAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACATCTTTTACTGTTCTGGATATTGACATTGAGTACGCTACAGTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCA
TTCTGGGTTATTTCTGCGAAATACAAGATCCATTTATCT

>TRINITY_DN109696_c1_g1_i10 from sample A5 / Example unspliced isoform:

TGTTGAACCAGTTGAAGTGCAAACCCAACTTGGTTATGATCTTGATCCCGAGCTTGCTGAATTCTTGAATAACCCCTTGAATGTCCGCAGATTATTATTGTTTGCCGAAAGAATGGGCAACATATTGGACGAGATAAAGGGGAAGCTGCTGGAATCGAGAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGCTGA
AGTTCTCTGTAAGCTATGGCGATAAGAAAATCAGCTTAGgtgatgaactattgcctgttcaagtgcaaagcgaacctatcctttttggagtttctggttatgatcttgatcccgagttaattgaaatcttgaataaccccttgaattaccccagattattatgggtaagtttaattcctttttcgtcaatatatgatatt
ttgtcaaattgaaattcatgttaacgatattttcataaacccataaacagGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTATTTGCACTGGTTA

Intron 15 GTTAGGTACG ... CAAGATCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.73	277.3	73	18
A2	29.37	161.7	65	19
A3	24.04	248.7	77	28
A4	29.04	195.3	73	15
A5	21.9	77.3	20	5
A6	23.87	1032	312	74
A7	23.04	1473.7	428	83
A8	23.23	1567.3	461	97
A9	22.8	1804.3	513	110
A10	21.33	1290.3	339	74

>TRINITY_DN109696_c1_g1_i4 from sample A5 / Example spliced isoform

TGTTGAACCAGTTGAAGTGCAAACCCAACTTGGTTATGATCTTGATCCCGAGCTTGCTGAATTCTTGAATAACCCCTTGAATGTCCGCAGATTATTATTGTTTGCCGAAAGAATGGGCAACATATTGGACGAGATAAAGGGGAAGCTGCTGGAATCGAGAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGCTGA
AGTTCTCTGTAAGCTATGGCGATAAGAAAATCAGCTTAGGTGATGAACTATTGCCTGTTCAAGTGCAAAGCGAACCTATCCTTTTTGGAGTTTCTGGTTATGATCTTGATCCCGAGTTAATTGAAATCTTGAATAACCCCTTGAATTACCCCAGATTATTATGGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTAT
TTGCACTGGTTATATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGATATTGCTGATCCACTTCGCCGTGATATAATTGTGGGCAACTCGCGGAGGAATTTCAGCACCAGAGATTTTGCTCAAGCTAATGCACTTGGACCGCCGCTGTCTGCTGCTTTTT
TTTACATCAAGAAGTAAGAAAATGAGGAGACTGATGATAGTGAATCATCCACATCCAGTATATGAAGTGGCCGAAAGAGATTGGATCAACCAGAATCAAGAAAGATGAAGTAGGCCATATACTCTGTGCCATTTGAATGGCTACACCATTTTCTTCATCAACATGTAACCGAAAAGATTCAAGAAAGATGAAGGAACAGA
AAAGATTTGAATGGCTACACCATTTTCTTCATGTCATCAACATCTTTTACTGTTCTGGATATTGACATTGAGTACGCTACAGTACCATTTATCTCTTATATGAAACATAATACAATATATTTTCATTCTGGGTTATTTCTGCGAAATACAAGATCCATTTATCT

>TRINITY_DN109696_c1_g1_i7 from sample A5 / Example unspliced isoform:

TGTTGAACCAGTTGAAGTGCAAACCCAACTTGGTTATGATCTTGATCCCGAGCTTGCTGAATTCTTGAATAACCCCTTGAATGTCCGCAGATTATTATTGTTTGCCGAAAGAATGGGCAACATATTGGACGAGATAAAGGGGAAGCTGCTGGAATCGAGAGTTATCGGAACTGTTGTCGATGATTTCAATCCTCGGCTGA
AGTTCTCTGTAAGCTATGGCGATAAGAAAATCAGCTTAGGTGATGAACTATTGCCTGTTCAAGTGCAAAGCGAACCTATCCTTTTTGGAGTTTCTGGTTATGATCTTGATCCCGAGTTAATTGAAATCTTGAATAACCCCTTGAATTACCCCAGATTATTATGGGTTATGACGGATCCTGCTCCTCCTGACGGCGAGTAT
TTGCACTGgttaggtacgtattttattagacaaacaagatccagGTTATATATCCTCGCAGGAGCTTTTTGCCTATGAACCTCCGAGGCCCGGTGCGCACATGCTGGTTTTCACGTTGTTCGAT